Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,144,158 C→A 100% intergenic (+253/+19) yghA → / ← exbD putative oxidoreductase/membrane spanning protein in TonB‑ExbB‑ExbD complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,144,1580CA99.5% 648.4 / ‑4.3 197intergenic (+253/+19)yghA/exbDputative oxidoreductase/membrane spanning protein in TonB‑ExbB‑ExbD complex
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (94/102);  total (95/102)
Fisher's exact test for biased strand distribution p-value = 4.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 198 total aligned reads displayed.

TGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGT  >  CP009273/3144013‑3144302
                                                                                                                                                 |                                                                                                                                                
   aaTAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATc                                                                                                                                             >  2:1947056/1‑148 (MQ=255)
   aaTAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATc                                                                                                                                             <  2:2267616/148‑1 (MQ=255)
   aaTAGATTTGGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTACTGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATc                                                                                                                                             <  1:557948/148‑1 (MQ=255)
    aTAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCa                                                                                                                                            >  1:2425982/1‑148 (MQ=255)
       gATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGc                                                                                                                                         >  1:722577/1‑148 (MQ=255)
        aTTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCa                                                                                                                                        <  1:1309997/148‑1 (MQ=255)
        aTTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCa                                                                                                                                        <  2:2057703/148‑1 (MQ=255)
        aTTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCa                                                                                                                                        >  2:2167407/1‑148 (MQ=255)
         ttttGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCaa                                                                                                                                       >  1:2650454/1‑148 (MQ=255)
           ttGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAAtt                                                                                                                                     >  1:1766916/1‑148 (MQ=255)
            tGTAGGCCTGATTAGCGTAGAGCATCAGTCATTGAGCAACTAATGACTGATTCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATTTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTc                                                                                                                                    <  1:540767/148‑1 (MQ=21)
              tAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCta                                                                                                                                  <  2:341298/148‑1 (MQ=255)
                ggCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCtact                                                                                                                                >  1:2254163/1‑148 (MQ=255)
                  ccTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCtacttt                                                                                                                              >  2:2385448/1‑148 (MQ=255)
                  ccTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCtacttt                                                                                                                              >  2:232436/1‑148 (MQ=255)
                   cTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCtacttta                                                                                                                             <  1:2017614/148‑1 (MQ=255)
                      aTAAGCGTAGCGCATCAGGCATCGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCtactttactt                                                                                                                          <  1:821643/148‑1 (MQ=255)
                        aaGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCg                                                                                                                        >  1:3340381/1‑148 (MQ=255)
                         aGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGc                                                                                                                       <  2:2750327/148‑1 (MQ=255)
                         aGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGc                                                                                                                       >  1:1755937/1‑148 (MQ=255)
                          gCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCt                                                                                                                      <  1:663233/148‑1 (MQ=255)
                          gCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCt                                                                                                                      <  2:1805912/148‑1 (MQ=255)
                           cGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCtt                                                                                                                     <  1:251257/148‑1 (MQ=255)
                             tAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTg                                                                                                                   <  2:1571181/148‑1 (MQ=255)
                              aGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTgg                                                                                                                  <  2:1896507/148‑1 (MQ=255)
                                cgcATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCg                                                                                                                >  2:1010899/1‑148 (MQ=255)
                                  cATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGt                                                                                                              <  1:282147/148‑1 (MQ=255)
                                     cAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTttc                                                                                                           >  2:1443964/1‑148 (MQ=255)
                                     cAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTttc                                                                                                           <  2:307015/148‑1 (MQ=255)
                                       ggCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTttctt                                                                                                         >  1:1325516/1‑148 (MQ=255)
                                        gCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTttcttc                                                                                                        >  2:2783263/1‑148 (MQ=255)
                                        gCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTttcttc                                                                                                        >  2:940486/1‑148 (MQ=255)
                                        gCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTttcttc                                                                                                        >  2:290551/1‑148 (MQ=255)
                                           ttGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGcc                                                                                                     >  2:2298367/1‑148 (MQ=255)
                                                cACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCa                                                                                                >  1:434437/1‑148 (MQ=255)
                                                   cTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGAc                                                                                             <  1:3148276/148‑1 (MQ=255)
                                                    tAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGAcc                                                                                            >  1:2114786/1‑148 (MQ=255)
                                                        gCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATc                                                                                        >  1:2316846/1‑148 (MQ=255)
                                                          cGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCtt                                                                                      <  1:2877810/148‑1 (MQ=255)
                                                           ggATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTc                                                                                     <  1:3010905/148‑1 (MQ=255)
                                                             aTGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAg                                                                                   <  1:3503043/148‑1 (MQ=255)
                                                                  tcgtGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAAc                                                                              >  2:2442323/1‑148 (MQ=255)
                                                                  tcgtGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAAc                                                                              <  1:664186/148‑1 (MQ=255)
                                                                   cgtGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAAcc                                                                             <  1:2070994/148‑1 (MQ=255)
                                                                   cgtGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAAcc                                                                             <  1:298231/148‑1 (MQ=255)
                                                                           ccTTATCCGGCCTACACACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCCGGTAACCCGCCTGAt                                                                     <  1:3365722/148‑1 (MQ=255)
                                                                           ccTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGAt                                                                     <  1:1625634/148‑1 (MQ=255)
                                                                             ttATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGc                                                                   >  2:311227/1‑148 (MQ=255)
                                                                               aTCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAg                                                                 <  2:1765194/148‑1 (MQ=255)
                                                                                 ccGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCg                                                               >  1:270184/1‑148 (MQ=255)
                                                                                 ccGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCg                                                               >  1:1442645/1‑148 (MQ=255)
                                                                                 ccGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATATTCAGGTAACCCGCCTGATGCAGCg                                                               <  1:2494392/148‑1 (MQ=255)
                                                                                      cTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATcc                                                          <  2:2496721/148‑1 (MQ=255)
                                                                                        aCAAACGCACGAAGATTCAACATGTTTCAATAGATTTTGTAGGCCTGATGAGCTTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCAt                                                        <  1:2421869/148‑1 (MQ=255)
                                                                                        aCAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCAt                                                        <  1:3248735/148‑1 (MQ=255)
                                                                                         cAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCAtt                                                       <  2:1815340/148‑1 (MQ=255)
                                                                                            aCGCACGAAGATTCATCATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTGTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTAcc                                                    <  2:1023730/148‑1 (MQ=255)
                                                                                            aCGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTAcc                                                    >  1:126600/1‑148 (MQ=255)
                                                                                                 cGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTtcat                                               <  2:530557/148‑1 (MQ=255)
                                                                                                      aTTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACg                                          >  2:758580/1‑148 (MQ=255)
                                                                                                       ttCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGt                                         <  1:3127301/148‑1 (MQ=255)
                                                                                                       ttCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGt                                         <  2:1630165/148‑1 (MQ=255)
                                                                                                       ttCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGt                                         >  2:1529967/1‑148 (MQ=255)
                                                                                                       ttCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGt                                         >  2:1529958/1‑148 (MQ=255)
                                                                                                       ttCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGt                                         >  1:1540482/1‑148 (MQ=255)
                                                                                                       ttCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGt                                         >  1:2838934/1‑148 (MQ=255)
                                                                                                        tCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGtc                                        >  2:943186/1‑148 (MQ=255)
                                                                                                         cAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGtct                                       >  1:3297674/1‑148 (MQ=255)
                                                                                                              tGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTaa                                  <  2:1256658/148‑1 (MQ=255)
                                                                                                               gTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAAt                                 >  2:3057784/1‑148 (MQ=255)
                                                                                                                ttGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATc                                >  2:243475/1‑148 (MQ=255)
                                                                                                                 tGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCg                               >  2:2088324/1‑148 (MQ=255)
                                                                                                                     aTAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACgg                           <  2:2889969/148‑1 (MQ=255)
                                                                                                                     aTAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACgg                           <  2:1096785/148‑1 (MQ=255)
                                                                                                                      tAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGt                          >  2:883917/1‑148 (MQ=255)
                                                                                                                       aGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGtt                         <  1:1399645/148‑1 (MQ=255)
                                                                                                                       aGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGtt                         <  1:3146021/148‑1 (MQ=255)
                                                                                                                         aTTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGtttt                       <  2:1658792/148‑1 (MQ=255)
                                                                                                                         aTTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGtttt                       >  1:3287152/1‑148 (MQ=255)
                                                                                                                          ttttGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTg                      <  2:3358204/148‑1 (MQ=255)
                                                                                                                             tGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTcc                   <  2:2650454/148‑1 (MQ=255)
                                                                                                                                aGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCt                >  2:2798860/1‑148 (MQ=255)
                                                                                                                                      gatgaGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGgaag          >  1:585461/1‑148 (MQ=255)
                                                                                                                                      gatgaGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTGTTGTCCGCTCGgaag          >  2:3222628/1‑148 (MQ=255)
                                                                                                                                       atgaGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGgaaga         <  1:158669/148‑1 (MQ=255)
                                                                                                                                       atgaGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGgaaga         <  1:1634043/148‑1 (MQ=255)
                                                                                                                                       atgaGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGgaaga         >  2:363140/1‑148 (MQ=255)
                                                                                                                                          aGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAagaaga      <  2:1375979/148‑1 (MQ=255)
                                                                                                                                          aGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAagaaga      <  2:817113/148‑1 (MQ=255)
                                                                                                                                           gCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGAt     >  1:3027443/1‑148 (MQ=255)
                                                                                                                                            cGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTCATCGACGGTTTTGTCCGCTCGGAAGAAGAtg    >  2:2919069/1‑148 (MQ=255)
                                                                                                                                            cGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTAACTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGACTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGAtg    <  2:1344024/148‑1 (MQ=255)
                                                                                                                                            cGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCGATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGAtg    <  2:1523413/148‑1 (MQ=255)
                                                                                                                                             gTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGAtgg   <  1:2794702/148‑1 (MQ=255)
                                                                                                                                             gTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGAtgg   <  1:920893/148‑1 (MQ=255)
                                                                                                                                             gTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGAtgg   <  1:1045629/148‑1 (MQ=255)
                                                                                                                                             gTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGAtgg   <  1:1010899/148‑1 (MQ=255)
                                                                                                                                              tAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGAtggt  >  2:3317026/1‑148 (MQ=255)
                                                                                                                                              tAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGAtggt  >  2:66519/1‑148 (MQ=255)
                                                                                                                                              tAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGAtggt  >  1:2327259/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGT  >  CP009273/3144013‑3144302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTGG  >  CP009273/3144010‑3144304
                                                                                                                                                    |                                                                                                                                                  
TGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAG                                                                                                                                                   >  SRR19973065.2367708/1‑150 (MQ=60)
TGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAG                                                                                                                                                   >  SRR19973065.4509/1‑150 (MQ=60)
TGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGACGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAG                                                                                                                                                   >  SRR19973065.3613/1‑150 (MQ=60)
 GTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGC                                                                                                                                                  >  SRR19973065.1641096/1‑150 (MQ=60)
  TTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCA                                                                                                                                                 <  SRR19973065.3201701/150‑1 (MQ=60)
  TTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCA                                                                                                                                                 <  SRR19973065.3263519/150‑1 (MQ=60)
   TGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCAT                                                                                                                                                >  SRR19973065.3092744/1‑150 (MQ=60)
    GCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATC                                                                                                                                               <  SRR19973065.2744462/150‑1 (MQ=60)
     CAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCA                                                                                                                                              >  SRR19973065.2441290/1‑150 (MQ=60)
      AATAGATTTGGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTACTGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAG                                                                                                                                             <  SRR19973065.561506/150‑1 (MQ=60)
        TAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGC                                                                                                                                           >  SRR19973065.727414/1‑150 (MQ=60)
        TAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGC                                                                                                                                           >  SRR19973065.2499397/1‑150 (MQ=60)
         AGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCA                                                                                                                                          >  SRR19973065.3535523/1‑150 (MQ=60)
          GATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAA                                                                                                                                         >  SRR19973065.2666799/1‑150 (MQ=60)
           ATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAAT                                                                                                                                        <  SRR19973065.1318720/150‑1 (MQ=60)
           ATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAAT                                                                                                                                        <  SRR19973065.1318644/150‑1 (MQ=60)
            TTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATT                                                                                                                                       >  SRR19973065.1778502/1‑150 (MQ=60)
            TTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATT                                                                                                                                       >  SRR19973065.1778291/1‑150 (MQ=60)
             TTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTC                                                                                                                                      <  SRR19973065.1580912/150‑1 (MQ=60)
               TGTAGGCCTGATTAGCGTAGAGCATCAGTCATTGAGCAACTAATGACTGATTCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATTTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTA                                                                                                                                    <  SRR19973065.544277/150‑1 (MQ=60)
               TGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTA                                                                                                                                    <  SRR19973065.2320927/150‑1 (MQ=60)
                 TAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACT                                                                                                                                  >  SRR19973065.835620/1‑150 (MQ=60)
                 TAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACT                                                                                                                                  >  SRR19973065.2268445/1‑150 (MQ=60)
                   GGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTT                                                                                                                                >  SRR19973065.3196800/1‑150 (MQ=60)
                      CTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCACTAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACT                                                                                                                             >  SRR19973065.2981251/1‑150 (MQ=60)
                      CTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACT                                                                                                                             <  SRR19973065.2030607/150‑1 (MQ=60)
                       TGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTT                                                                                                                            >  SRR19973065.1385054/1‑150 (MQ=60)
                        GATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTC                                                                                                                           <  SRR19973065.2046773/150‑1 (MQ=60)
                         ATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCG                                                                                                                          >  SRR19973065.3360864/1‑150 (MQ=60)
                         ATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCG                                                                                                                          >  SRR19973065.3360789/1‑150 (MQ=60)
                         ATAAGCGTAGCGCATCAGGCATCGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCG                                                                                                                          <  SRR19973065.827037/150‑1 (MQ=60)
                          TAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGC                                                                                                                         >  SRR19973065.1767482/1‑150 (MQ=60)
                             GCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTT                                                                                                                      <  SRR19973065.667632/150‑1 (MQ=60)
                             GCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTT                                                                                                                      <  SRR19973065.2438956/150‑1 (MQ=60)
                              CGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTG                                                                                                                     <  SRR19973065.1819127/150‑1 (MQ=60)
                              CGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTG                                                                                                                     <  SRR19973065.253014/150‑1 (MQ=60)
                                     CATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTT                                                                                                              <  SRR19973065.284015/150‑1 (MQ=60)
                                        CAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTT                                                                                                           >  SRR19973065.1334277/1‑150 (MQ=60)
                                         AGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTC                                                                                                          <  SRR19973065.2181330/150‑1 (MQ=60)
                                            CATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCC                                                                                                       >  SRR19973065.554398/1‑150 (MQ=60)
                                             ATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCG                                                                                                      >  SRR19973065.3499023/1‑150 (MQ=60)
                                                 AGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCA                                                                                                  <  SRR19973065.2896023/150‑1 (MQ=60)
                                                 AGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCA                                                                                                  >  SRR19973065.437459/1‑150 (MQ=60)
                                                 AGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCA                                                                                                  >  SRR19973065.534026/1‑150 (MQ=60)
                                                     CCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACC                                                                                              >  SRR19973065.2128396/1‑150 (MQ=60)
                                                      CTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCT                                                                                             <  SRR19973065.3167390/150‑1 (MQ=60)
                                                       TAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTA                                                                                            >  SRR19973065.2095519/1‑150 (MQ=60)
                                                         ATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATC                                                                                          >  SRR19973065.1536826/1‑150 (MQ=60)
                                                         ATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATC                                                                                          >  SRR19973065.2331371/1‑150 (MQ=60)
                                                           GCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTT                                                                                        <  SRR19973065.3355896/150‑1 (MQ=60)
                                                           GCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTT                                                                                        <  SRR19973065.1393427/150‑1 (MQ=60)
                                                           GCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTT                                                                                        <  SRR19973065.1600395/150‑1 (MQ=60)
                                                             CGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCA                                                                                      <  SRR19973065.2895434/150‑1 (MQ=60)
                                                              GGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAG                                                                                     <  SRR19973065.3029344/150‑1 (MQ=60)
                                                               GATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGG                                                                                    <  SRR19973065.1704592/150‑1 (MQ=60)
                                                                ATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGT                                                                                   <  SRR19973065.3524313/150‑1 (MQ=60)
                                                                     TCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCC                                                                              <  SRR19973065.668590/150‑1 (MQ=60)
                                                                     TCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCC                                                                              <  SRR19973065.668592/150‑1 (MQ=60)
                                                                     TCGTGAACGCCTTAACCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCC                                                                              <  SRR19973065.668572/150‑1 (MQ=60)
                                                                      CGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCG                                                                             <  SRR19973065.2084205/150‑1 (MQ=60)
                                                                      CGTGAACGCCTTATCCGGCCAACAACCGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCG                                                                             <  SRR19973065.300157/150‑1 (MQ=60)
                                                                              CCTTATCCGGCCTACACACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCCGGTAACCCGCCTGATGC                                                                     <  SRR19973065.3386302/150‑1 (MQ=60)
                                                                              CCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGC                                                                     <  SRR19973065.1636528/150‑1 (MQ=60)
                                                                                  ATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCG                                                                 >  SRR19973065.272010/1‑150 (MQ=60)
                                                                                  ATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCG                                                                 >  SRR19973065.1451984/1‑150 (MQ=60)
                                                                                    CCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATATTCAGGTAACCCGCCTGATGCAGCGTA                                                               <  SRR19973065.2510086/150‑1 (MQ=60)
                                                                                          TACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATT                                                         >  SRR19973065.2863776/1‑150 (MQ=60)
                                                                                           ACAAACGCACGAAGATTCAACATGTTTCAATAGATTTTGTAGGCCTGATGAGCTTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTA                                                        <  SRR19973065.2437146/150‑1 (MQ=60)
                                                                                           ACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTA                                                        <  SRR19973065.3268423/150‑1 (MQ=60)
                                                                                           ACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTA                                                        <  SRR19973065.3268361/150‑1 (MQ=60)
                                                                                            CAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTAC                                                       <  SRR19973065.2595683/150‑1 (MQ=60)
                                                                                             AAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACC                                                      >  SRR19973065.127691/1‑150 (MQ=60)
                                                                                                CGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTC                                                   <  SRR19973065.1443188/150‑1 (MQ=60)
                                                                                                    CGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCA                                               <  SRR19973065.803048/150‑1 (MQ=60)
                                                                                                     GAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAA                                              >  SRR19973065.284602/1‑150 (MQ=60)
                                                                                                      AAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAAC                                             >  SRR19973065.3166254/1‑150 (MQ=60)
                                                                                                      AAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAAC                                             <  SRR19973065.2525234/150‑1 (MQ=60)
                                                                                                        GATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGT                                           >  SRR19973065.1550664/1‑150 (MQ=60)
                                                                                                        GATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGT                                           >  SRR19973065.2856410/1‑150 (MQ=60)
                                                                                                          TTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCT                                         <  SRR19973065.3146398/150‑1 (MQ=60)
                                                                                                          TTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCT                                         <  SRR19973065.3146339/150‑1 (MQ=60)
                                                                                                          TTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCT                                         >  SRR19973065.3317756/1‑150 (MQ=60)
                                                                                                          TTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCT                                         <  SRR19973065.302643/150‑1 (MQ=60)
                                                                                                          TTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCC                                         >  SRR19973065.3317543/1‑150 (MQ=60)
                                                                                                            CAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCG                                       >  SRR19973065.664937/1‑150 (MQ=60)
                                                                                                            CAACATGGTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCCTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCG                                       <  SRR19973065.3033693/150‑1 (MQ=60)
                                                                                                                ATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAAT                                   <  SRR19973065.1001384/150‑1 (MQ=60)
                                                                                                                 TGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATC                                  <  SRR19973065.234125/150‑1 (MQ=60)
                                                                                                                 TGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATC                                  <  SRR19973065.2400408/150‑1 (MQ=60)
                                                                                                                          AGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTT                         <  SRR19973065.3165129/150‑1 (MQ=60)
                                                                                                                          AGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTT                         <  SRR19973065.1408825/150‑1 (MQ=60)
                                                                                                                          AGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTT                         >  SRR19973065.3307150/1‑150 (MQ=60)
                                                                                                                          AGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCACCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTT                         >  SRR19973065.1086513/1‑150 (MQ=60)
                                                                                                                            ATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGT                       >  SRR19973065.2715964/1‑150 (MQ=60)
                                                                                                                                   AGGCCTGATGAGCGTAGAGCATCAGGCAATTCGACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCG                <  SRR19973065.1284603/150‑1 (MQ=60)
                                                                                                                                       CTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAG            >  SRR19973065.588112/1‑150 (MQ=60)
                                                                                                                                       CTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAG            >  SRR19973065.589104/1‑150 (MQ=60)
                                                                                                                                         GATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAA          >  SRR19973065.282365/1‑150 (MQ=60)
                                                                                                                                          ATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAG         <  SRR19973065.1645012/150‑1 (MQ=60)
                                                                                                                                          ATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAG         <  SRR19973065.159982/150‑1 (MQ=60)
                                                                                                                                            GAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGAT       >  SRR19973065.3045993/1‑150 (MQ=60)
                                                                                                                                               CGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGT    >  SRR19973065.2341820/1‑150 (MQ=60)
                                                                                                                                                GTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTG   <  SRR19973065.926927/150‑1 (MQ=60)
                                                                                                                                                GTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTG   <  SRR19973065.2812018/150‑1 (MQ=60)
                                                                                                                                                GTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTG   <  SRR19973065.1052545/150‑1 (MQ=60)
                                                                                                                                                GTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTG   <  SRR19973065.1017602/150‑1 (MQ=60)
                                                                                                                                                 TAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGACGAAGATGGTGG  >  SRR19973065.2602297/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                  
TGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTGG  >  CP009273/3144010‑3144304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑