Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,144,158 C→A 100% intergenic (+253/+19) yghA → / ← exbD putative oxidoreductase/membrane spanning protein in TonB‑ExbB‑ExbD complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,144,1580CA100.0% 539.5 / NA 162intergenic (+253/+19)yghA/exbDputative oxidoreductase/membrane spanning protein in TonB‑ExbB‑ExbD complex
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (96/66);  total (96/66)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 164 total aligned reads displayed.

TGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGT  >  CP009273/3144013‑3144302
                                                                                                                                                 |                                                                                                                                                
tGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAgagc                                                                                                                                                <  2:119550/148‑1 (MQ=255)
 gCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCa                                                                                                                                               <  1:900001/148‑1 (MQ=255)
  cAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCAt                                                                                                                                              <  1:334878/148‑1 (MQ=255)
  cAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCAt                                                                                                                                              <  2:855968/148‑1 (MQ=255)
   aaTAGAATTGGGAGGCCTGATAAGCGTAGCGCATCAGGCATAGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATc                                                                                                                                             <  1:1050035/148‑1 (MQ=37)
     tAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAg                                                                                                                                           <  1:1115461/148‑1 (MQ=255)
     tAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAg                                                                                                                                           >  1:1602173/1‑148 (MQ=255)
      aGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAgg                                                                                                                                          <  2:3011327/148‑1 (MQ=255)
      aGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAgg                                                                                                                                          <  2:3011312/148‑1 (MQ=255)
      aGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAgg                                                                                                                                          >  2:2735671/1‑148 (MQ=255)
        aTTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCa                                                                                                                                        >  2:2237431/1‑148 (MQ=255)
          tttGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCacg                                                                                                                                      >  1:865075/1‑146 (MQ=255)
          tttGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAAt                                                                                                                                      >  1:1741856/1‑148 (MQ=255)
             gTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAAGGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCt                                                                                                                                   <  2:1915881/148‑1 (MQ=255)
                ggCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCtact                                                                                                                                >  1:2850591/1‑148 (MQ=255)
                ggCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGAGTAGAGCATCAGGCAATTCtact                                                                                                                                >  1:2336425/1‑148 (MQ=255)
                     gATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCtactttact                                                                                                                           >  1:411872/1‑148 (MQ=255)
                      aTAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCtactttactt                                                                                                                          <  2:289392/148‑1 (MQ=255)
                      aTAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCtactttactt                                                                                                                          <  1:2734121/148‑1 (MQ=255)
                        aaGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCg                                                                                                                        <  2:1903408/148‑1 (MQ=255)
                           cGTAGCGCATCAGGCATTTAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCtt                                                                                                                     <  2:3034264/148‑1 (MQ=255)
                           cGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCtt                                                                                                                     >  2:1271763/1‑148 (MQ=255)
                           cGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCtt                                                                                                                     >  2:1957900/1‑148 (MQ=255)
                           cGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCtt                                                                                                                     >  2:1958088/1‑148 (MQ=255)
                           cGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCtt                                                                                                                     >  2:506531/1‑148 (MQ=255)
                           cGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCtt                                                                                                                     <  1:2520840/148‑1 (MQ=255)
                           cGTAGCGCATCAGGCATTGAGCACCTAAGGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCtt                                                                                                                     <  2:2611282/148‑1 (MQ=255)
                              aGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTgg                                                                                                                  <  1:2606668/148‑1 (MQ=255)
                                cgcATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCGTTGGCg                                                                                                                >  2:1828162/1‑148 (MQ=255)
                                  cATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGt                                                                                                              >  2:282790/1‑148 (MQ=255)
                                    tCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGttt                                                                                                            >  1:2436234/1‑148 (MQ=255)
                                     cAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTttc                                                                                                           <  1:1236853/148‑1 (MQ=255)
                                     cAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTttc                                                                                                           >  2:764332/1‑148 (MQ=255)
                                      aGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTttct                                                                                                          <  1:1727616/148‑1 (MQ=255)
                                               gCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGAcc                                                                                                 <  1:2432810/148‑1 (MQ=255)
                                               gCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGAcc                                                                                                 <  1:2432186/148‑1 (MQ=255)
                                                cACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCa                                                                                                >  2:474455/1‑148 (MQ=255)
                                                  ccTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGa                                                                                              >  2:1025709/1‑148 (MQ=255)
                                                  ccTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGa                                                                                              >  2:1460951/1‑148 (MQ=255)
                                                   cTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGAc                                                                                             <  1:2006535/148‑1 (MQ=255)
                                                    tAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGAcc                                                                                            >  1:2290464/1‑148 (MQ=255)
                                                     aaTGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCt                                                                                           <  2:3254683/148‑1 (MQ=255)
                                                        gCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATc                                                                                        <  2:1183431/148‑1 (MQ=255)
                                                               gCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGt                                                                                 >  1:3272102/1‑148 (MQ=255)
                                                                  tcgtGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAAc                                                                              <  2:746678/148‑1 (MQ=255)
                                                                      gAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGc                                                                          >  1:193565/1‑148 (MQ=255)
                                                                       aaCGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGcc                                                                         <  1:868006/148‑1 (MQ=255)
                                                                        aCGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCt                                                                        >  1:1798243/1‑148 (MQ=255)
                                                                         cGCCTGATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGNATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTg                                                                       <  1:1681536/148‑1 (MQ=255)
                                                                          gCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGa                                                                      <  2:46175/148‑1 (MQ=255)
                                                                           ccTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGCTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGAt                                                                     >  2:3069736/1‑148 (MQ=255)
                                                                             ttATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGc                                                                   <  2:80009/148‑1 (MQ=255)
                                                                             ttATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGc                                                                   >  1:1794013/1‑148 (MQ=255)
                                                                             ttATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGc                                                                   <  1:154747/148‑1 (MQ=255)
                                                                             ttATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCGATCTTCAGGTAACCCGCCTGATGc                                                                   <  1:154819/148‑1 (MQ=255)
                                                                                tCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGc                                                                >  1:775810/1‑148 (MQ=255)
                                                                                tCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCATGATGAGCGAAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGc                                                                >  1:775802/1‑148 (MQ=255)
                                                                                 ccGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCg                                                               >  1:2339406/1‑148 (MQ=255)
                                                                                  cGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGt                                                              >  1:585337/1‑148 (MQ=255)
                                                                                    gCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTAt                                                            >  1:2016497/1‑148 (MQ=255)
                                                                                       tACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCa                                                         >  2:2883824/1‑148 (MQ=255)
                                                                                       tACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCa                                                         >  2:1338162/1‑148 (MQ=255)
                                                                                       tACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCa                                                         <  2:2047981/148‑1 (MQ=255)
                                                                                        aCAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCAt                                                        <  2:665486/148‑1 (MQ=255)
                                                                                         cAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCAtt                                                       >  1:1934383/1‑148 (MQ=255)
                                                                                           aaCGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTAc                                                     >  2:1208568/1‑148 (MQ=255)
                                                                                             cGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCt                                                   >  1:1487833/1‑148 (MQ=255)
                                                                                                   aaGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCGGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTtcatca                                             >  1:291479/1‑148 (MQ=255)
                                                                                                   aaGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTtcatca                                             >  1:1946793/1‑148 (MQ=255)
                                                                                                     gATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAAc                                           >  1:507675/1‑148 (MQ=255)
                                                                                                        tCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGtc                                        >  2:2689399/1‑148 (MQ=255)
                                                                                                         cAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGtct                                       >  2:1164932/1‑148 (MQ=255)
                                                                                                           aCATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCg                                     >  2:1830700/1‑148 (MQ=255)
                                                                                                            cATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGt                                    >  2:944133/1‑148 (MQ=255)
                                                                                                               gTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAAt                                 >  1:1973995/1‑148 (MQ=255)
                                                                                                               gTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAAt                                 >  2:1303946/1‑148 (MQ=255)
                                                                                                               gTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAAt                                 >  2:1685980/1‑148 (MQ=255)
                                                                                                                 tGCAATAGATTTTGTAGGCCTGATGAGCGTATAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCg                               >  2:2385881/1‑148 (MQ=255)
                                                                                                                 tGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCg                               >  2:2385986/1‑148 (MQ=255)
                                                                                                                 tGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCg                               >  2:1741335/1‑148 (MQ=255)
                                                                                                                  gCAATAGATTTGGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACGTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGa                              <  2:1647106/148‑1 (MQ=255)
                                                                                                                     aTAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACgg                           >  1:3089329/1‑148 (MQ=255)
                                                                                                                      tAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGt                          >  1:3122064/1‑148 (MQ=255)
                                                                                                                        gATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGttt                        <  1:1271763/148‑1 (MQ=255)
                                                                                                                         aTTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGtttt                       >  1:1823798/1‑148 (MQ=255)
                                                                                                                            ttGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTc                    <  2:2775766/148‑1 (MQ=255)
                                                                                                                                 ggCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTc               >  1:2971300/1‑148 (MQ=255)
                                                                                                                                  gCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCg              >  1:3164455/1‑148 (MQ=255)
                                                                                                                                    cTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGga            <  1:2103832/148‑1 (MQ=255)
                                                                                                                                     tgatgaGCGTAGAGCATCAGGCAATTCTACTTTACGTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGgaa           <  2:865075/148‑1 (MQ=255)
                                                                                                                                       atgaGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGgaaga         <  2:3156512/148‑1 (MQ=255)
                                                                                                                                       atgaGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGgaaga         >  1:268142/1‑148 (MQ=255)
                                                                                                                                         gaGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGaagaag       >  2:2328104/1‑148 (MQ=255)
                                                                                                                                         gaGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGaagaag       >  1:1782727/1‑148 (MQ=255)
                                                                                                                                         gaGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGaagaag       <  1:764332/148‑1 (MQ=255)
                                                                                                                                            cGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGAtg    <  2:2229019/148‑1 (MQ=255)
                                                                                                                                            cGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGAtg    >  2:1513815/1‑148 (MQ=255)
                                                                                                                                            cGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGAtg    <  2:2850591/148‑1 (MQ=255)
                                                                                                                                              tAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGAtggt  >  2:340573/1‑148 (MQ=255)
                                                                                                                                              tAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGAtggt  >  2:340326/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGT  >  CP009273/3144013‑3144302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTG  >  CP009273/3144009‑3144303
                                                                                                                                                     |                                                                                                                                                 
ATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGA                                                                                                                                                   >  SRR19973070.3219411/1‑150 (MQ=60)
ATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGA                                                                                                                                                   >  SRR19973070.3219261/1‑150 (MQ=60)
 TGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAG                                                                                                                                                  <  SRR19973070.40091/150‑1 (MQ=60)
     GCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATC                                                                                                                                              <  SRR19973070.906377/150‑1 (MQ=60)
      CAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCA                                                                                                                                             <  SRR19973070.337319/150‑1 (MQ=60)
       AATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAG                                                                                                                                            <  SRR19973070.3242498/150‑1 (MQ=60)
       AATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAG                                                                                                                                            <  SRR19973070.2223699/150‑1 (MQ=60)
       AATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAG                                                                                                                                            >  SRR19973070.1613356/1‑150 (MQ=60)
       AATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAG                                                                                                                                            >  SRR19973070.1613348/1‑150 (MQ=60)
       AATAGAATTGGGAGGCCTGATAAGCGTAGCGCATCAGGCATAGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAG                                                                                                                                            <  SRR19973070.1057425/150‑1 (MQ=60)
        ATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGG                                                                                                                                           >  SRR19973070.2789078/1‑150 (MQ=60)
         TAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGC                                                                                                                                          <  SRR19973070.1123082/150‑1 (MQ=60)
           GATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAA                                                                                                                                        >  SRR19973070.132812/1‑150 (MQ=60)
            ATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAcg                                                                                                                                       >  SRR19973070.871110/1‑148 (MQ=60)
            ATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAAT                                                                                                                                       >  SRR19973070.872285/1‑150 (MQ=60)
            ATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAAT                                                                                                                                       >  SRR19973070.1753935/1‑150 (MQ=60)
            ATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGAGTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAAT                                                                                                                                       >  SRR19973070.1753932/1‑150 (MQ=60)
              TTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTC                                                                                                                                     <  SRR19973070.1765817/150‑1 (MQ=60)
               TTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCT                                                                                                                                    >  SRR19973070.2012383/1‑150 (MQ=60)
                  TAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACT                                                                                                                                 >  SRR19973070.2869052/1‑150 (MQ=60)
                  TAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACT                                                                                                                                 >  SRR19973070.776761/1‑150 (MQ=60)
                  TAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACT                                                                                                                                 >  SRR19973070.678923/1‑150 (MQ=60)
                  TAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGAGTAGAGCATCAGGCAATTCTACT                                                                                                                                 >  SRR19973070.2351931/1‑150 (MQ=60)
                       CTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACT                                                                                                                            >  SRR19973070.414879/1‑150 (MQ=60)
                          ATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCG                                                                                                                         <  SRR19973070.2751739/150‑1 (MQ=60)
                               CGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTG                                                                                                                    <  SRR19973070.2537867/150‑1 (MQ=60)
                               CGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTG                                                                                                                    <  SRR19973070.2537278/150‑1 (MQ=60)
                               CGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTG                                                                                                                    <  SRR19973070.776023/150‑1 (MQ=60)
                                  AGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCG                                                                                                                 <  SRR19973070.2623684/150‑1 (MQ=60)
                                  AGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCG                                                                                                                 <  SRR19973070.1001051/150‑1 (MQ=60)
                                      CATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCACCATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTGTGGCGGTTT                                                                                                             >  SRR19973070.409714/1‑150 (MQ=60)
                                      CATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTT                                                                                                             >  SRR19973070.2452209/1‑150 (MQ=60)
                                      CATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTT                                                                                                             >  SRR19973070.2452160/1‑150 (MQ=60)
                                        TCAGGCATTGAGCACCTACTGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCT                                                                                                           <  SRR19973070.432778/150‑1 (MQ=60)
                                         CAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTT                                                                                                          <  SRR19973070.1245514/150‑1 (MQ=60)
                                         CAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTT                                                                                                          <  SRR19973070.364961/150‑1 (MQ=60)
                                         CAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTT                                                                                                          <  SRR19973070.1245343/150‑1 (MQ=60)
                                          AGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTC                                                                                                         <  SRR19973070.1739583/150‑1 (MQ=60)
                                                  AGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCA                                                                                                 >  SRR19973070.1970013/1‑150 (MQ=60)
                                                   GCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAG                                                                                                <  SRR19973070.2448148/150‑1 (MQ=60)
                                                   GCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAG                                                                                                <  SRR19973070.2448773/150‑1 (MQ=60)
                                                      CCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACC                                                                                             >  SRR19973070.2305691/1‑150 (MQ=60)
                                                       CTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCT                                                                                            <  SRR19973070.2020125/150‑1 (MQ=60)
                                                       CTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCT                                                                                            <  SRR19973070.2020068/150‑1 (MQ=60)
                                                        TAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTA                                                                                           >  SRR19973070.3057999/1‑150 (MQ=60)
                                                               GGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAG                                                                                    <  SRR19973070.2442255/150‑1 (MQ=60)
                                                                 ATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGT                                                                                  >  SRR19973070.3293138/1‑150 (MQ=60)
                                                                     GTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACC                                                                              >  SRR19973070.3259811/1‑150 (MQ=60)
                                                                        GTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGC                                                                           >  SRR19973070.195056/1‑150 (MQ=60)
                                                                          GAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCT                                                                         >  SRR19973070.1810660/1‑150 (MQ=60)
                                                                          GAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCT                                                                         >  SRR19973070.1809439/1‑150 (MQ=60)
                                                                           AACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTG                                                                        <  SRR19973070.874067/150‑1 (MQ=60)
                                                                             CGCCTGATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGNATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGAT                                                                      <  SRR19973070.1693059/150‑1 (MQ=60)
                                                                               CCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGC                                                                    >  SRR19973070.1806405/1‑150 (MQ=60)
                                                                                 TTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAG                                                                  <  SRR19973070.156039/150‑1 (MQ=60)
                                                                                 TTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCGATCTTCAGGTAACCCGCCTGATGCAG                                                                  <  SRR19973070.156112/150‑1 (MQ=60)
                                                                                 GTATCCGGCCTACAATCGCACGAAGTTTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAG                                                                  <  SRR19973070.1539685/150‑1 (MQ=60)
                                                                                  TATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGC                                                                 >  SRR19973070.781241/1‑150 (MQ=60)
                                                                                  TATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCATGATGAGCGAAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGC                                                                 >  SRR19973070.781233/1‑150 (MQ=60)
                                                                                   ATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCG                                                                >  SRR19973070.2354930/1‑150 (MQ=60)
                                                                                    TCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGT                                                               >  SRR19973070.589371/1‑150 (MQ=60)
                                                                                      CGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTAT                                                             >  SRR19973070.2030138/1‑150 (MQ=60)
                                                                                           TACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATT                                                        >  SRR19973070.1947394/1‑150 (MQ=60)
                                                                                           TACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATT                                                        >  SRR19973070.1948625/1‑150 (MQ=60)
                                                                                               AACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCT                                                    >  SRR19973070.1498245/1‑150 (MQ=60)
                                                                                                     CGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCGGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCA                                              >  SRR19973070.293470/1‑150 (MQ=60)
                                                                                                     CGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCA                                              >  SRR19973070.1959867/1‑150 (MQ=60)
                                                                                                       AAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAAC                                            >  SRR19973070.511181/1‑150 (MQ=60)
                                                                                                              AACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGT                                     >  SRR19973070.2172446/1‑150 (MQ=60)
                                                                                                                 ATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAAT                                  >  SRR19973070.1987342/1‑150 (MQ=60)
                                                                                                                  TGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATC                                 <  SRR19973070.477808/150‑1 (MQ=60)
                                                                                                                       CAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGG                            >  SRR19973070.3109205/1‑150 (MQ=60)
                                                                                                                        AATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGT                           >  SRR19973070.3142217/1‑150 (MQ=60)
                                                                                                                         ATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTT                          >  SRR19973070.3121975/1‑150 (MQ=60)
                                                                                                                           AGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTT                        >  SRR19973070.1682252/1‑150 (MQ=60)
                                                                                                                           AGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTT                        >  SRR19973070.1836345/1‑150 (MQ=60)
                                                                                                                            GATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTG                       <  SRR19973070.1280662/150‑1 (MQ=60)
                                                                                                                             ATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGT                      >  SRR19973070.2352466/1‑150 (MQ=60)
                                                                                                                                  GTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCT                 <  SRR19973070.1878907/150‑1 (MQ=60)
                                                                                                                                   TAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTC                >  SRR19973070.2990386/1‑150 (MQ=60)
                                                                                                                                    AGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCG               >  SRR19973070.3184929/1‑150 (MQ=60)
                                                                                                                                       CCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAA            >  SRR19973070.2901802/1‑150 (MQ=60)
                                                                                                                                        CTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAG           <  SRR19973070.2117947/150‑1 (MQ=60)
                                                                                                                                        CTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAG           <  SRR19973070.2117948/150‑1 (MQ=60)
                                                                                                                                         TGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGA          >  SRR19973070.269763/1‑150 (MQ=60)
                                                                                                                                         TGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGA          >  SRR19973070.269908/1‑150 (MQ=60)
                                                                                                                                           ATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAG        >  SRR19973070.844429/1‑150 (MQ=60)
                                                                                                                                           ATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAG        >  SRR19973070.1795061/1‑150 (MQ=60)
                                                                                                                                             GAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGAT      <  SRR19973070.769701/150‑1 (MQ=60)
                                                                                                                                                 GTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTG  >  SRR19973070.778249/1‑150 (MQ=60)
                                                                                                                                                 GTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTG  >  SRR19973070.778300/1‑150 (MQ=60)
                                                                                                                                                 GTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTG  >  SRR19973070.3188791/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                 
ATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTG  >  CP009273/3144009‑3144303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑