Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,144,158 C→A 100% intergenic (+253/+19) yghA → / ← exbD putative oxidoreductase/membrane spanning protein in TonB‑ExbB‑ExbD complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,144,1580CA100.0% 630.6 / ‑6.7 192intergenic (+253/+19)yghA/exbDputative oxidoreductase/membrane spanning protein in TonB‑ExbB‑ExbD complex
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (102/89);  minor base T (1/0);  total (103/89)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 192 total aligned reads displayed.

ATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGT  >  CP009273/3144017‑3144302
                                                                                                                                             |                                                                                                                                                
aTAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCa                                                                                                                                            >  1:3510861/1‑148 (MQ=255)
 tAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATCGATGTTGTAGGCCTGATGAGCGTAGAGCATCAg                                                                                                                                           >  2:2008897/1‑148 (MQ=255)
 tAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAg                                                                                                                                           >  1:2994349/1‑148 (MQ=255)
 tAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAg                                                                                                                                           >  2:2008914/1‑148 (MQ=255)
  aGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAgg                                                                                                                                          <  2:897659/148‑1 (MQ=255)
     ttttGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCaa                                                                                                                                       >  1:3343416/1‑148 (MQ=255)
      tttGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAAt                                                                                                                                      >  2:1735386/1‑148 (MQ=255)
      tttGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAAt                                                                                                                                      >  1:3401863/1‑148 (MQ=255)
      tttGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGAGTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAAt                                                                                                                                      >  2:1179813/1‑148 (MQ=255)
        tGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTc                                                                                                                                    >  2:3354604/1‑148 (MQ=255)
          tAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCta                                                                                                                                  <  2:641669/148‑1 (MQ=255)
           aGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCtac                                                                                                                                 <  2:1513896/148‑1 (MQ=255)
            ggCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCtact                                                                                                                                >  1:551762/1‑148 (MQ=255)
            ggCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCtact                                                                                                                                <  1:2256365/148‑1 (MQ=255)
             gCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCtactt                                                                                                                               >  1:1891471/1‑148 (MQ=255)
             gCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCtactt                                                                                                                               >  2:536033/1‑148 (MQ=255)
                tGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCtactttac                                                                                                                            >  1:1020309/1‑148 (MQ=255)
                tGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCtactttac                                                                                                                            >  1:2049446/1‑148 (MQ=255)
                tGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCCGATGAGCGTAGAGCATCAGGCAATTCtactttac                                                                                                                            >  1:2048816/1‑148 (MQ=255)
                  aTAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCtactttactt                                                                                                                          >  1:1828491/1‑148 (MQ=255)
                    aaGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCg                                                                                                                        <  2:926569/148‑1 (MQ=255)
                    aaGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCg                                                                                                                        <  2:1159100/148‑1 (MQ=255)
                    aaGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCg                                                                                                                        >  1:2903132/1‑148 (MQ=255)
                     aGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGc                                                                                                                       >  1:513800/1‑148 (MQ=255)
                     aGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGc                                                                                                                       >  2:1602186/1‑148 (MQ=255)
                      gCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCt                                                                                                                      >  2:2401672/1‑148 (MQ=255)
                            cgcATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCg                                                                                                                >  1:2913430/1‑148 (MQ=255)
                             gcATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCgg                                                                                                               <  1:3127640/148‑1 (MQ=255)
                             gcATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCgg                                                                                                               <  2:2298311/148‑1 (MQ=255)
                              cATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGt                                                                                                              <  2:284590/148‑1 (MQ=255)
                              cATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGt                                                                                                              <  2:1435306/148‑1 (MQ=255)
                                tCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGttt                                                                                                            >  2:1912739/1‑148 (MQ=255)
                                 cAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTttc                                                                                                           >  2:2990658/1‑148 (MQ=255)
                                 cAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTttc                                                                                                           >  1:431802/1‑148 (MQ=255)
                                  aGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTttct                                                                                                          <  2:562564/148‑1 (MQ=255)
                                   ggCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTttctt                                                                                                         <  2:2882763/148‑1 (MQ=255)
                                     cATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCg                                                                                                       >  1:3539567/1‑148 (MQ=255)
                                       ttGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGcc                                                                                                     >  2:3021896/1‑148 (MQ=255)
                                       ttGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGcc                                                                                                     >  1:2411991/1‑148 (MQ=255)
                                        tGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTCCTTCGCTTTGGCGGTTTCTTCGCCg                                                                                                    >  1:2411826/1‑148 (MQ=255)
                                        tGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCg                                                                                                    >  1:3245190/1‑148 (MQ=255)
                                               cTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGAc                                                                                             <  1:1179813/148‑1 (MQ=255)
                                                tAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGAcc                                                                                            >  2:1881434/1‑148 (MQ=255)
                                                        gATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCa                                                                                    <  2:323978/148‑1 (MQ=255)
                                                         aTGCGTCGTTAACTCCTTATCCGGCCTACATAAGCACGAAGATTCAACATGTTGAAATATATTTTGTAGGACTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGTTTTCTTCGCCGACCAGAACTATCTTCAg                                                                                   <  2:3189239/148‑1 (MQ=255)
                                                         aTGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAg                                                                                   <  1:721812/148‑1 (MQ=255)
                                                           gCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGt                                                                                 >  2:3530622/1‑148 (MQ=255)
                                                            cttcttGAACGCCTGATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTAATTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTa                                                                                <  1:185749/143‑1 (MQ=255)
                                                               cgtGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAAcc                                                                             >  2:3393970/1‑148 (MQ=255)
                                                                  gAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGc                                                                          <  2:822603/148‑1 (MQ=255)
                                                                   aaCGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGcc                                                                         <  2:3514991/148‑1 (MQ=255)
                                                                   aaCGCCTTATCCGGCCAACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGcc                                                                         <  1:2504618/148‑1 (MQ=255)
                                                                     cGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTg                                                                       <  1:3031153/148‑1 (MQ=255)
                                                                     cGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTg                                                                       >  1:2208062/1‑148 (MQ=255)
                                                                     cGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTg                                                                       <  1:1124143/148‑1 (MQ=255)
                                                                     cGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTg                                                                       >  1:2208458/1‑148 (MQ=255)
                                                                      gCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGa                                                                      <  1:1278496/148‑1 (MQ=255)
                                                                        cTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATg                                                                    >  1:3405162/1‑148 (MQ=255)
                                                                           aTCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAg                                                                 <  2:3441945/148‑1 (MQ=255)
                                                                              cGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGt                                                              <  2:970230/148‑1 (MQ=255)
                                                                                gCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTAt                                                            <  2:1246347/148‑1 (MQ=255)
                                                                                  cTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATcc                                                          >  2:937014/1‑148 (MQ=255)
                                                                                   tACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCa                                                         >  1:949416/1‑148 (MQ=255)
                                                                                    aCAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCAt                                                        >  1:3339847/1‑148 (MQ=255)
                                                                                    aCAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCAt                                                        <  2:1299791/148‑1 (MQ=255)
                                                                                     cAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCAtt                                                       >  2:3581392/1‑148 (MQ=255)
                                                                                     cAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCAtt                                                       <  2:2603066/148‑1 (MQ=255)
                                                                                        aCGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTAcc                                                    <  2:3401863/148‑1 (MQ=255)
                                                                                        aCGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTAcc                                                    >  1:3530381/1‑148 (MQ=255)
                                                                                             cGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTtcat                                               <  1:2382509/148‑1 (MQ=255)
                                                                                               aaGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTtcatca                                             <  1:2679738/148‑1 (MQ=255)
                                                                                               aaGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTtcatca                                             <  1:1016600/148‑1 (MQ=255)
                                                                                                 gATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAAc                                           >  2:708741/1‑148 (MQ=255)
                                                                                                  aTTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACg                                          >  1:931327/1‑148 (MQ=255)
                                                                                                  aTTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACg                                          >  1:2419164/1‑148 (MQ=255)
                                                                                                  aTTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACg                                          >  1:605191/1‑148 (MQ=255)
                                                                                                  aTTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACg                                          >  1:2418848/1‑148 (MQ=255)
                                                                                                   ttCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGt                                         <  1:3478968/148‑1 (MQ=255)
                                                                                                    tCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGtc                                        <  1:3220384/148‑1 (MQ=255)
                                                                                                     cAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGtct                                       >  1:2474462/1‑148 (MQ=255)
                                                                                                       aCATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCg                                     <  2:2348669/148‑1 (MQ=255)
                                                                                                        cATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGt                                    >  2:2124515/1‑148 (MQ=255)
                                                                                                         aTGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTa                                   <  1:3354604/148‑1 (MQ=255)
                                                                                                          tGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTaa                                  >  2:3745553/1‑148 (MQ=255)
                                                                                                          tGTTGCAATAGATTTGGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTaa                                  <  2:3309602/148‑1 (MQ=255)
                                                                                                             tGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCg                               <  2:2266231/148‑1 (MQ=255)
                                                                                                              gCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGa                              <  2:3539567/148‑1 (MQ=255)
                                                                                                              gCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGa                              <  1:1701715/148‑1 (MQ=255)
                                                                                                                 aTAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACgg                           <  2:903923/148‑1 (MQ=255)
                                                                                                                 aTAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACgg                           >  1:789000/1‑148 (MQ=255)
                                                                                                                  tAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGt                          >  1:861029/1‑148 (MQ=255)
                                                                                                                    gATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGttt                        >  2:1731487/1‑148 (MQ=255)
                                                                                                                    gATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGttt                        >  2:3625177/1‑148 (MQ=255)
                                                                                                                     aTTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGtttt                       <  2:2750991/148‑1 (MQ=255)
                                                                                                                            aGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCt                <  1:2516714/148‑1 (MQ=255)
                                                                                                                                     gaGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGaagaag       <  2:551762/148‑1 (MQ=255)
                                                                                                                                       gCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGAt     <  2:2411991/148‑1 (MQ=255)
                                                                                                                                       gCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGAt     <  2:3343416/148‑1 (MQ=255)
                                                                                                                                        cGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGAtg    >  1:1964046/1‑148 (MQ=255)
                                                                                                                                          tAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGAtggt  >  1:2755791/1‑148 (MQ=255)
                                                                                                                                             |                                                                                                                                                
ATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGT  >  CP009273/3144017‑3144302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTGGT  >  CP009273/3144010‑3144305
                                                                                                                                                    |                                                                                                                                                   
TGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAG                                                                                                                                                    <  SRR19973069.3072166/150‑1 (MQ=60)
     CAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCA                                                                                                                                               >  SRR19973069.3533484/1‑150 (MQ=60)
     CAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCA                                                                                                                                               >  SRR19973069.3413425/1‑150 (MQ=60)
      AATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAG                                                                                                                                              >  SRR19973069.3014230/1‑150 (MQ=60)
         AGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCA                                                                                                                                           <  SRR19973069.503669/150‑1 (MQ=60)
          GATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAA                                                                                                                                          >  SRR19973069.3365303/1‑150 (MQ=60)
           ATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAAT                                                                                                                                         >  SRR19973069.3424050/1‑150 (MQ=60)
                GTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTAC                                                                                                                                    <  SRR19973069.3468211/150‑1 (MQ=60)
                 TAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACT                                                                                                                                   >  SRR19973069.556305/1‑150 (MQ=60)
                 TAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACT                                                                                                                                   <  SRR19973069.292279/150‑1 (MQ=60)
                 TAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACT                                                                                                                                   >  SRR19973069.555605/1‑150 (MQ=60)
                 TAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACT                                                                                                                                   >  SRR19973069.555614/1‑150 (MQ=60)
                  AGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTT                                                                                                                                  >  SRR19973069.1904587/1‑150 (MQ=60)
                  AGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTT                                                                                                                                  >  SRR19973069.1904556/1‑150 (MQ=60)
                   GGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTT                                                                                                                                 <  SRR19973069.2271614/150‑1 (MQ=60)
                     CCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCACTTCTACTTTAC                                                                                                                               >  SRR19973069.2063078/1‑150 (MQ=60)
                     CCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTAC                                                                                                                               >  SRR19973069.1027364/1‑150 (MQ=60)
                     CCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTAC                                                                                                                               >  SRR19973069.2063674/1‑150 (MQ=60)
                     CCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCCGATGAGCGTAGAGCATCAGGCAATTCTACTTTAC                                                                                                                               >  SRR19973069.2063039/1‑150 (MQ=60)
                       TGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTT                                                                                                                             >  SRR19973069.1841311/1‑150 (MQ=60)
                        GATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTC                                                                                                                            <  SRR19973069.2762352/150‑1 (MQ=60)
                         ATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCG                                                                                                                           <  SRR19973069.1421643/150‑1 (MQ=60)
                         ATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCG                                                                                                                           >  SRR19973069.2922315/1‑150 (MQ=60)
                         ATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCG                                                                                                                           <  SRR19973069.2662491/150‑1 (MQ=60)
                          TAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGC                                                                                                                          >  SRR19973069.517456/1‑150 (MQ=60)
                             GCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTT                                                                                                                       <  SRR19973069.2477246/150‑1 (MQ=60)
                                 AGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACTAAGATTCAACATGTTGCAATATATTTTGTAGGCCTGATGAGCGTATAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCG                                                                                                                   >  SRR19973069.3036708/1‑150 (MQ=60)
                                 AGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCG                                                                                                                   >  SRR19973069.2932707/1‑150 (MQ=60)
                                   CGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATGCTACTTTACTTCGCTTTGGCGGT                                                                                                                 >  SRR19973069.803753/1‑150 (MQ=60)
                                    GCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTT                                                                                                                <  SRR19973069.3148161/150‑1 (MQ=60)
                                      ATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTC                                                                                                              >  SRR19973069.434975/1‑150 (MQ=60)
                                          GGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCG                                                                                                          >  SRR19973069.3562528/1‑150 (MQ=60)
                                            CATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCNATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCC                                                                                                        >  SRR19973069.1949883/1‑150 (MQ=60)
                                            CATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCC                                                                                                        >  SRR19973069.2428261/1‑150 (MQ=60)
                                            CATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCC                                                                                                        >  SRR19973069.2428295/1‑150 (MQ=60)
                                             ATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTCCTTCGCTTTGGCGGTTTCTTCGCCG                                                                                                       >  SRR19973069.2428127/1‑150 (MQ=60)
                                             ATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCG                                                                                                       >  SRR19973069.3266431/1‑150 (MQ=60)
                                             ATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCG                                                                                                       >  SRR19973069.2357697/1‑150 (MQ=60)
                                                      CTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCT                                                                                              <  SRR19973069.1187967/150‑1 (MQ=60)
                                                        ACTGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTAT                                                                                            <  SRR19973069.321173/150‑1 (MQ=60)
                                                        AATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTAT                                                                                            <  SRR19973069.1480240/150‑1 (MQ=60)
                                                          TGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCT                                                                                          <  SRR19973069.2807724/150‑1 (MQ=60)
                                                              GGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAG                                                                                      <  SRR19973069.748994/150‑1 (MQ=60)
                                                                ATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGT                                                                                    <  SRR19973069.726902/150‑1 (MQ=60)
                                                                ATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGT                                                                                    <  SRR19973069.725960/150‑1 (MQ=60)
                                                                   cttctTGAACGCCTGATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTAATTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAAC                                                                                 <  SRR19973069.187282/145‑1 (MQ=60)
                                                                    GTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACC                                                                                <  SRR19973069.99116/150‑1 (MQ=60)
                                                                          AACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTG                                                                          <  SRR19973069.2521504/150‑1 (MQ=60)
                                                                          AACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTG                                                                          <  SRR19973069.2521315/150‑1 (MQ=60)
                                                                          AACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTG                                                                          >  SRR19973069.2223035/1‑150 (MQ=60)
                                                                          AACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTG                                                                          >  SRR19973069.2223431/1‑150 (MQ=60)
                                                                          AACGCCTTATCCGGCCAACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTG                                                                          <  SRR19973069.2521330/150‑1 (MQ=60)
                                                                            CGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGAT                                                                        <  SRR19973069.1132072/150‑1 (MQ=60)
                                                                            CGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGAT                                                                        <  SRR19973069.1131983/150‑1 (MQ=60)
                                                                            CGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGAT                                                                        <  SRR19973069.3051254/150‑1 (MQ=60)
                                                                             GCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATG                                                                       >  SRR19973069.3427368/1‑150 (MQ=60)
                                                                             GCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATG                                                                       <  SRR19973069.1287067/150‑1 (MQ=60)
                                                                              CCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGC                                                                      >  SRR19973069.1276336/1‑150 (MQ=60)
                                                                                        CCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCA                                                            >  SRR19973069.955804/1‑150 (MQ=60)
                                                                                         CTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCAT                                                           >  SRR19973069.3361736/1‑150 (MQ=60)
                                                                                         CTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCAT                                                           >  SRR19973069.3361713/1‑150 (MQ=60)
                                                                                         CTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCAT                                                           >  SRR19973069.3361701/1‑150 (MQ=60)
                                                                                            CAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTAC                                                        <  SRR19973069.2246750/150‑1 (MQ=60)
                                                                                             AAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACC                                                       >  SRR19973069.3553236/1‑150 (MQ=60)
                                                                                                    CGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCA                                                <  SRR19973069.2398666/150‑1 (MQ=60)
                                                                                                    CGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCA                                                <  SRR19973069.2398939/150‑1 (MQ=60)
                                                                                                      AAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAAC                                              <  SRR19973069.1023615/150‑1 (MQ=60)
                                                                                                      AAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAAC                                              <  SRR19973069.2697704/150‑1 (MQ=60)
                                                                                                       AGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACG                                             >  SRR19973069.2435186/1‑150 (MQ=60)
                                                                                                       AGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACG                                             >  SRR19973069.609263/1‑150 (MQ=60)
                                                                                                       AGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACG                                             >  SRR19973069.2435506/1‑150 (MQ=60)
                                                                                                       AGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACG                                             >  SRR19973069.1833935/1‑150 (MQ=60)
                                                                                                       AGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACG                                             >  SRR19973069.2435200/1‑150 (MQ=60)
                                                                                                       AGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACG                                             >  SRR19973069.2435187/1‑150 (MQ=60)
                                                                                                       AGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACG                                             >  SRR19973069.937644/1‑150 (MQ=60)
                                                                                                       AGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACG                                             >  SRR19973069.937717/1‑150 (MQ=60)
                                                                                                         ATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTC                                           >  SRR19973069.2086207/1‑150 (MQ=60)
                                                                                                          TTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCT                                          >  SRR19973069.1045982/1‑150 (MQ=60)
                                                                                                          TTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCT                                          >  SRR19973069.2966675/1‑150 (MQ=60)
                                                                                                          TTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCT                                          <  SRR19973069.3501448/150‑1 (MQ=60)
                                                                                                          TTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCT                                          >  SRR19973069.2491051/1‑150 (MQ=60)
                                                                                                           TCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTC                                         <  SRR19973069.3241415/150‑1 (MQ=60)
                                                                                                           TCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTC                                         <  SRR19973069.3241172/150‑1 (MQ=60)
                                                                                                            CAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCG                                        <  SRR19973069.3553481/150‑1 (MQ=60)
                                                                                                             AACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGT                                       >  SRR19973069.905342/1‑150 (MQ=60)
                                                                                                                ATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAAT                                    <  SRR19973069.3376556/150‑1 (MQ=60)
                                                                                                                ATGTTGCAAGAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAAT                                    <  SRR19973069.3376451/150‑1 (MQ=60)
                                                                                                                     GCAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACG                               <  SRR19973069.1713619/150‑1 (MQ=60)
                                                                                                                      CAATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGG                              >  SRR19973069.794599/1‑150 (MQ=60)
                                                                                                                       AATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGT                             >  SRR19973069.3842179/1‑150 (MQ=60)
                                                                                                                       AATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGT                             >  SRR19973069.867045/1‑150 (MQ=60)
                                                                                                                       AATAGATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGT                             >  SRR19973069.3426798/1‑150 (MQ=60)
                                                                                                                           GATTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTG                         <  SRR19973069.1864835/150‑1 (MQ=60)
                                                                                                                             TTTTGTAGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTC                       <  SRR19973069.3542646/150‑1 (MQ=60)
                                                                                                                                   AGGCCTGATGAGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCG                 <  SRR19973069.2533462/150‑1 (MQ=60)
                                                                                                                                             AGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATG       >  SRR19973069.1977669/1‑150 (MQ=60)
                                                                                                                                             AGCGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATG       >  SRR19973069.1977840/1‑150 (MQ=60)
                                                                                                                                               CGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGT     >  SRR19973069.2774182/1‑150 (MQ=60)
                                                                                                                                               CGTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGT     >  SRR19973069.3055205/1‑150 (MQ=60)
                                                                                                                                                GTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTG    <  SRR19973069.3248051/150‑1 (MQ=60)
                                                                                                                                                GTAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTG    >  SRR19973069.1222782/1‑150 (MQ=60)
                                                                                                                                                 TAGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTGG   >  SRR19973069.3001134/1‑150 (MQ=60)
                                                                                                                                                  AGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTGGT  >  SRR19973069.1367696/1‑150 (MQ=60)
                                                                                                                                                  AGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTGGT  >  SRR19973069.3283641/1‑150 (MQ=60)
                                                                                                                                                  AGAGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTGGT  >  SRR19973069.3283630/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                   
TGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAGCGCATCAGGCAATTCTACTTTACTTCGCTTTGGCGGTTTCTTCGCCGACCAGACCTATCTTCAGGTAACCCGCCTGATGCAGCGTATCCATTACCTTCATCAACGTCTCGTAATCGACGGTTTTGTCCGCTCGGAAGAAGATGGTGGT  >  CP009273/3144010‑3144305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑