Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F181 I0 R1 1262 323.1 10297870 97.5% 10040423 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 474,138 G→T 93.9% intergenic (‑65/+99) ylaB ← / ← ylaC putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase/DUF1449 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273474,1380GT93.9% 764.4 / 34.6 255intergenic (‑65/+99)ylaB/ylaCputative membrane‑anchored cyclic‑di‑GMP phosphodiesterase/DUF1449 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (11/5);  new base T (123/116);  total (134/121)
Fisher's exact test for biased strand distribution p-value = 2.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 259 total aligned reads displayed.

CAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGA  >  CP009273/473990‑474284
                                                                                                                                                    |                                                                                                                                                  
cagccagATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGgaga                                                                                                                                                   >  2:1529641/1‑150 (MQ=255)
       aTGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAaggag                                                                                                                                            <  2:4360404/150‑1 (MQ=255)
       aTGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAaggag                                                                                                                                            <  2:457717/150‑1 (MQ=255)
       aTGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAaggag                                                                                                                                            >  1:4986360/1‑150 (MQ=255)
          cTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTa                                                                                                                                         <  2:4052677/150‑1 (MQ=255)
          cTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTa                                                                                                                                         <  1:3016482/150‑1 (MQ=255)
          cTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTa                                                                                                                                         >  1:2644992/1‑150 (MQ=255)
           ttAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTag                                                                                                                                        >  2:122830/1‑150 (MQ=255)
              aGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGc                                                                                                                                     >  2:2933782/1‑150 (MQ=255)
                ccGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTg                                                                                                                                   <  1:2954216/150‑1 (MQ=255)
                   aCAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTa                                                                                                                                <  1:4738308/150‑1 (MQ=255)
                    cAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGTATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTAt                                                                                                                               >  2:3951091/1‑150 (MQ=255)
                     aGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTAtt                                                                                                                              >  1:1496181/1‑150 (MQ=255)
                     aGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTAtt                                                                                                                              <  1:339583/150‑1 (MQ=255)
                         agcaATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCAt                                                                                                                          <  1:4370434/150‑1 (MQ=255)
                             aTACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAg                                                                                                                      <  1:174778/150‑1 (MQ=255)
                               aCTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTg                                                                                                                    <  2:4683834/150‑1 (MQ=255)
                                 tGAAAGAATCAGTACGCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGAt                                                                                                                  <  2:4782159/150‑1 (MQ=255)
                                   aaaGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATgg                                                                                                                >  2:4347209/1‑150 (MQ=255)
                                    aaGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATggg                                                                                                               >  2:3160673/1‑150 (MQ=255)
                                     aGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGt                                                                                                              >  1:2272330/1‑150 (MQ=255)
                                       aaTCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTgg                                                                                                            >  2:693587/1‑150 (MQ=255)
                                       aaTCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTgg                                                                                                            <  1:1080884/150‑1 (MQ=255)
                                       aaTCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGGGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTgg                                                                                                            >  1:2051211/1‑150 (MQ=255)
                                          cAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGaaa                                                                                                         <  1:2343400/150‑1 (MQ=255)
                                           aGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAAc                                                                                                        <  2:368999/150‑1 (MQ=255)
                                             tACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGa                                                                                                      >  2:14294/1‑150 (MQ=255)
                                              aCTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGAt                                                                                                     <  2:1003119/150‑1 (MQ=255)
                                                tCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTCTATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCa                                                                                                   >  2:3717821/1‑150 (MQ=255)
                                                 cccGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCAt                                                                                                  <  2:3840381/150‑1 (MQ=255)
                                                  ccGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCAtt                                                                                                 <  2:4986360/150‑1 (MQ=255)
                                                    gAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGt                                                                                               >  2:2444573/1‑150 (MQ=255)
                                                      aaTAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCa                                                                                             <  1:1036093/150‑1 (MQ=255)
                                                      aaTAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCa                                                                                             <  2:3280834/150‑1 (MQ=255)
                                                        tAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCAtt                                                                                           >  1:2329327/1‑150 (MQ=255)
                                                         aaGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCAttt                                                                                          <  1:5023836/150‑1 (MQ=255)
                                                              cGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCa                                                                                     >  2:4235629/1‑150 (MQ=255)
                                                              cGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCa                                                                                     >  2:1884900/1‑150 (MQ=255)
                                                                 ccAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGgc                                                                                  >  1:3680084/1‑150 (MQ=255)
                                                                  cAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGgcg                                                                                 <  2:2562032/150‑1 (MQ=255)
                                                                      tGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCaga                                                                             >  2:381573/1‑150 (MQ=255)
                                                                       gTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCagag                                                                            <  1:2249375/150‑1 (MQ=255)
                                                                        tCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGc                                                                           <  2:575855/150‑1 (MQ=255)
                                                                         cGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCt                                                                          <  2:3248004/150‑1 (MQ=255)
                                                                          gtgtTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCtt                                                                         >  2:2139778/1‑150 (MQ=255)
                                                                          gtgtTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCtt                                                                         <  1:1344878/150‑1 (MQ=255)
                                                                          gtgtTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCtt                                                                         <  1:1587817/150‑1 (MQ=255)
                                                                           tgtTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCttt                                                                        <  2:275567/150‑1 (MQ=255)
                                                                           tgtTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCttt                                                                        >  1:3425045/1‑150 (MQ=255)
                                                                             ttCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTAc                                                                      <  1:654478/150‑1 (MQ=255)
                                                                             ttCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTAc                                                                      <  1:3409145/150‑1 (MQ=255)
                                                                             ttCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTAc                                                                      <  1:4082104/150‑1 (MQ=255)
                                                                             ttCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTAc                                                                      <  2:2712688/150‑1 (MQ=255)
                                                                              tctcACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACg                                                                     >  2:545463/1‑150 (MQ=255)
                                                                              tctcACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACg                                                                     >  2:3512153/1‑150 (MQ=255)
                                                                              tctcACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACg                                                                     >  2:3201991/1‑150 (MQ=255)
                                                                              tctcACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGATGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACg                                                                     >  2:4993019/1‑150 (MQ=255)
                                                                                 cACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCt                                                                  <  1:2391159/150‑1 (MQ=255)
                                                                                  aCCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTg                                                                 >  1:3819718/1‑150 (MQ=255)
                                                                                  aCCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTg                                                                 >  2:376808/1‑150 (MQ=255)
                                                                                   cgtgCATATACTTATTAACAGTGTTTAGGGATTGTATATATGATTGTGTAAATAGGCTGGCTGGATATTAGGAGTTTGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCTTTGTCATTTGACCTGGCGCAGAGCTTTACGCCttg                                                                <  1:3545802/147‑3 (MQ=255)
                                                                                    cAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGt                                                               >  2:2673859/1‑150 (MQ=255)
                                                                                    cAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGt                                                               >  1:932700/1‑150 (MQ=255)
                                                                                      gCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTAt                                                             >  2:1702076/1‑150 (MQ=255)
                                                                                      gCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTAt                                                             <  1:1211033/150‑1 (MQ=255)
                                                                                         tatCCTTATTAACACAGTGTAGGGAAAGTAAATCTGATTGAGGAAAAAGGCTGGCGGGAGATAAGGAGGTAGAGCTGGTATTTCATTAAGTGATGGGTGGAAACCATCATAGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTa                                                          >  1:1094967/1‑150 (MQ=255)
                                                                                             cTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAAccc                                                      >  1:2574810/1‑150 (MQ=255)
                                                                                                 ttaACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTAATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAAc                                                  >  1:5061703/1‑150 (MQ=255)
                                                                                                  taACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTCTACGCCTGGTATGTAACCCTAACt                                                 <  2:743248/150‑1 (MQ=255)
                                                                                                           gtAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGtt                                        <  1:1816450/150‑1 (MQ=255)
                                                                                                           gtAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGtt                                        >  2:1024706/1‑150 (MQ=255)
                                                                                                            tAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTg                                       >  2:2334166/1‑150 (MQ=255)
                                                                                                             aGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGa                                      <  2:2912098/150‑1 (MQ=255)
                                                                                                              gggAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGAt                                     <  2:1356011/150‑1 (MQ=255)
                                                                                                                  aaGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCgg                                 >  2:824870/1‑150 (MQ=255)
                                                                                                                  aaGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCgg                                 <  1:1031947/150‑1 (MQ=255)
                                                                                                                     tataTCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGgcgc                              >  1:19814/1‑150 (MQ=255)
                                                                                                                       tatCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCgcga                            <  1:1505491/150‑1 (MQ=255)
                                                                                                                         tCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCgcgagc                          <  2:3373011/150‑1 (MQ=255)
                                                                                                                         tCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCgcgagc                          <  2:1980609/150‑1 (MQ=255)
                                                                                                                         tCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCgcgagc                          <  2:5104460/150‑1 (MQ=255)
                                                                                                                          cTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCgcgagcg                         >  1:1560675/1‑150 (MQ=255)
                                                                                                                           tGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCgcgagcga                        >  1:4443808/1‑150 (MQ=255)
                                                                                                                           tGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCgcgagcga                        >  1:2436867/1‑150 (MQ=255)
                                                                                                                             aTTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAgg                      >  2:2818401/1‑150 (MQ=255)
                                                                                                                               tgtgGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGt                    <  1:3150995/150‑1 (MQ=255)
                                                                                                                               tgtgGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGt                    <  1:1846291/150‑1 (MQ=255)
                                                                                                                                 tgGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTaa                  <  1:3961959/150‑1 (MQ=255)
                                                                                                                                 tgGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTaa                  <  1:2933686/150‑1 (MQ=255)
                                                                                                                                  ggAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTaaa                 <  1:3643461/150‑1 (MQ=255)
                                                                                                                                    aaaaaGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTaaaaa               >  2:5130713/1‑150 (MQ=255)
                                                                                                                                    aaaaaGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTaaaaa               <  2:3666675/150‑1 (MQ=255)
                                                                                                                                       aaGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAatat            >  2:762663/1‑150 (MQ=255)
                                                                                                                                        aGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATc           <  2:1496181/150‑1 (MQ=255)
                                                                                                                                          gctggctgGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGt         >  1:4159994/1‑150 (MQ=255)
                                                                                                                                            tggctggATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTaa       >  2:2500619/1‑150 (MQ=255)
                                                                                                                                             ggctggATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTaaa      >  1:1876906/1‑150 (MQ=255)
                                                                                                                                             ggctggATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTaaa      >  2:2673328/1‑150 (MQ=255)
                                                                                                                                               ctggATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTaaaaa    >  1:1122740/1‑150 (MQ=255)
                                                                                                                                                tggATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAg   >  2:1436475/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGA  >  CP009273/473990‑474284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAG  >  CP009273/473991‑474287
                                                                                                                                                   |                                                                                                                                                     
AGCCAGTTGCTTAAGCCGACAGGCAGCTTTACTGAAAGAATCAGTACTACCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATAT                                                                                                                                                     <  SRR19973077.2308460/150‑1 (MQ=60)
AGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATAT                                                                                                                                                     >  SRR19973077.4264911/1‑150 (MQ=60)
  CCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAA                                                                                                                                                   >  SRR19973077.5060567/1‑150 (MQ=60)
  CCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAA                                                                                                                                                   >  SRR19973077.2717525/1‑150 (MQ=60)
    AGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGG                                                                                                                                                 <  SRR19973077.1178703/150‑1 (MQ=60)
      ATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCGGGATATAAGGAG                                                                                                                                               >  SRR19973077.4995224/1‑150 (MQ=60)
       TGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGG                                                                                                                                              >  SRR19973077.4577908/1‑150 (MQ=60)
        GCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGT                                                                                                                                             >  SRR19973077.235850/1‑150 (MQ=60)
         CTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTA                                                                                                                                            <  SRR19973077.4380036/150‑1 (MQ=60)
         CTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTA                                                                                                                                            >  SRR19973077.2649693/1‑150 (MQ=60)
         CTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTA                                                                                                                                            <  SRR19973077.3021795/150‑1 (MQ=60)
          TTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAG                                                                                                                                           <  SRR19973077.1044306/150‑1 (MQ=60)
               CCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTG                                                                                                                                      <  SRR19973077.626065/150‑1 (MQ=60)
               CCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTG                                                                                                                                      <  SRR19973077.4597943/150‑1 (MQ=60)
               CCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTG                                                                                                                                      <  SRR19973077.2593466/150‑1 (MQ=60)
               CCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTG                                                                                                                                      <  SRR19973077.3061160/150‑1 (MQ=60)
               CCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTG                                                                                                                                      <  SRR19973077.2959427/150‑1 (MQ=60)
               CCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTG                                                                                                                                      <  SRR19973077.2959445/150‑1 (MQ=60)
                  ACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTA                                                                                                                                   <  SRR19973077.4746654/150‑1 (MQ=60)
                    AGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATT                                                                                                                                 >  SRR19973077.1498792/1‑150 (MQ=60)
                    AGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATT                                                                                                                                 <  SRR19973077.340183/150‑1 (MQ=60)
                     GGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTT                                                                                                                                >  SRR19973077.1504150/1‑150 (MQ=60)
                        AGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCAT                                                                                                                             <  SRR19973077.4378160/150‑1 (MQ=60)
                            ATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAG                                                                                                                         <  SRR19973077.175111/150‑1 (MQ=60)
                                  AAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGG                                                                                                                   >  SRR19973077.328731/1‑150 (MQ=60)
                                    AGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGT                                                                                                                 >  SRR19973077.2276230/1‑150 (MQ=60)
                                    AGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGT                                                                                                                 >  SRR19973077.2276441/1‑150 (MQ=60)
                                    AGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGT                                                                                                                 >  SRR19973077.2276326/1‑150 (MQ=60)
                                     GAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTG                                                                                                                >  SRR19973077.1811763/1‑150 (MQ=60)
                                      AATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGG                                                                                                               <  SRR19973077.1082789/150‑1 (MQ=60)
                                      AATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGG                                                                                                               >  SRR19973077.2054701/1‑150 (MQ=60)
                                      AATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGGGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGG                                                                                                               >  SRR19973077.2054774/1‑150 (MQ=60)
                                         CAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAA                                                                                                            <  SRR19973077.2347540/150‑1 (MQ=60)
                                           GTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACG                                                                                                          <  SRR19973077.4226427/150‑1 (MQ=60)
                                                     AATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCA                                                                                                <  SRR19973077.1037925/150‑1 (MQ=60)
                                                       TAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATT                                                                                              >  SRR19973077.2333437/1‑150 (MQ=60)
                                                       TAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATT                                                                                              <  SRR19973077.3695092/150‑1 (MQ=60)
                                                        AAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTT                                                                                             <  SRR19973077.5032765/150‑1 (MQ=60)
                                                        AAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTT                                                                                             >  SRR19973077.3684450/1‑150 (MQ=60)
                                                           GCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGAC                                                                                          <  SRR19973077.3416761/150‑1 (MQ=60)
                                                           GCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGAC                                                                                          <  SRR19973077.3416867/150‑1 (MQ=60)
                                                              GACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAG                                                                                       >  SRR19973077.26286/1‑150 (MQ=60)
                                                              GACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAG                                                                                       <  SRR19973077.2380002/150‑1 (MQ=60)
                                                               ACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGG                                                                                      <  SRR19973077.1446187/150‑1 (MQ=60)
                                                               ACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGG                                                                                      <  SRR19973077.1446980/150‑1 (MQ=60)
                                                                CCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGC                                                                                     >  SRR19973077.3686571/1‑150 (MQ=60)
                                                                 CAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCG                                                                                    <  SRR19973077.3539810/150‑1 (MQ=60)
                                                                      GTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAG                                                                               <  SRR19973077.2253322/150‑1 (MQ=60)
                                                                      GTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAG                                                                               <  SRR19973077.1881401/150‑1 (MQ=60)
                                                                         GTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTT                                                                            <  SRR19973077.4156803/150‑1 (MQ=60)
                                                                         GTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTT                                                                            <  SRR19973077.1590579/150‑1 (MQ=60)
                                                                         GTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTT                                                                            <  SRR19973077.1347246/150‑1 (MQ=60)
                                                                          TGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTT                                                                           >  SRR19973077.3431058/1‑150 (MQ=60)
                                                                            TTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTAC                                                                         <  SRR19973077.655646/150‑1 (MQ=60)
                                                                            TTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTAC                                                                         <  SRR19973077.3415138/150‑1 (MQ=60)
                                                                            TTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTAC                                                                         <  SRR19973077.4089316/150‑1 (MQ=60)
                                                                             TCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACG                                                                        <  SRR19973077.3974665/150‑1 (MQ=60)
                                                                             TCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACG                                                                        >  SRR19973077.5009270/1‑150 (MQ=60)
                                                                                CACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCT                                                                     <  SRR19973077.2395386/150‑1 (MQ=60)
                                                                                CACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCGGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCT                                                                     >  SRR19973077.3413535/1‑150 (MQ=60)
                                                                                 ACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTG                                                                    >  SRR19973077.3826427/1‑150 (MQ=60)
                                                                                  cgtgcatatacttattaacagtgtttagggattGTATATATGATTGTGTAAATAGGCTGGCTGGATATTAGGAGTTTGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCTTTGTCATTTGACCTGGCGCAGAGCTTTACGCCTTG                                                                   <  SRR19973077.3552091/117‑1 (MQ=60)
                                                                                   CAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGT                                                                  >  SRR19973077.934360/1‑150 (MQ=60)
                                                                                     GCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTAT                                                                <  SRR19973077.3856130/150‑1 (MQ=60)
                                                                                     GCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTAT                                                                <  SRR19973077.49101/150‑1 (MQ=60)
                                                                                     GCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTAT                                                                <  SRR19973077.1213137/150‑1 (MQ=60)
                                                                                     GCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTAT                                                                <  SRR19973077.1213153/150‑1 (MQ=60)
                                                                                        TATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTA                                                             >  SRR19973077.2475504/1‑150 (MQ=60)
                                                                                        TATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTA                                                             >  SRR19973077.1096974/1‑150 (MQ=60)
                                                                                        TATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTA                                                             >  SRR19973077.1096999/1‑150 (MQ=60)
                                                                                        TATCCTTATTAACACAGTGTAGGGAAAGTAAATCTGATTGAGGAAAAAGGCTGGCGGGAGATAAGGAGGTAGAGCTGGTATTTCATTAAGTGATGGGTGGAAACCATCATAGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTA                                                             >  SRR19973077.1096891/1‑150 (MQ=60)
                                                                                           CCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACC                                                          <  SRR19973077.796784/150‑1 (MQ=60)
                                                                                            CTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCC                                                         >  SRR19973077.2579362/1‑150 (MQ=60)
                                                                                               ATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAA                                                      <  SRR19973077.1556855/150‑1 (MQ=60)
                                                                                               ATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAA                                                      <  SRR19973077.1556802/150‑1 (MQ=60)
                                                                                                TTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAAC                                                     <  SRR19973077.595911/150‑1 (MQ=60)
                                                                                                TTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTAATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAAC                                                     >  SRR19973077.5070679/1‑150 (MQ=60)
                                                                                                 TAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACT                                                    <  SRR19973077.1853431/150‑1 (MQ=60)
                                                                                                    CACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAA                                                 <  SRR19973077.2107509/150‑1 (MQ=60)
                                                                                                    CACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAA                                                 <  SRR19973077.2107503/150‑1 (MQ=60)
                                                                                                     ACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAG                                                <  SRR19973077.1088542/150‑1 (MQ=60)
                                                                                                          GTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTT                                           <  SRR19973077.1819615/150‑1 (MQ=60)
                                                                                                          GTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTT                                           <  SRR19973077.123072/150‑1 (MQ=60)
                                                                                                          GTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTT                                           <  SRR19973077.1819637/150‑1 (MQ=60)
                                                                                                            AGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGA                                         <  SRR19973077.3565552/150‑1 (MQ=60)
                                                                                                               GAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTC                                      <  SRR19973077.1824799/150‑1 (MQ=60)
                                                                                                                 AAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGG                                    <  SRR19973077.1033772/150‑1 (MQ=60)
                                                                                                                  AGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGC                                   >  SRR19973077.3919978/1‑150 (MQ=60)
                                                                                                                    TATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGC                                 >  SRR19973077.1076062/1‑150 (MQ=60)
                                                                                                                    TATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGC                                 >  SRR19973077.19862/1‑150 (MQ=60)
                                                                                                                    TATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGC                                 >  SRR19973077.20158/1‑150 (MQ=60)
                                                                                                                     ATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGATGATTCGGCGCG                                >  SRR19973077.3654698/1‑150 (MQ=60)
                                                                                                                      TATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGA                               <  SRR19973077.1508117/150‑1 (MQ=60)
                                                                                                                        TCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGC                             >  SRR19973077.2431059/1‑150 (MQ=60)
                                                                                                                        TCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGC                             <  SRR19973077.769303/150‑1 (MQ=60)
                                                                                                                         CTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCG                            >  SRR19973077.1034845/1‑150 (MQ=60)
                                                                                                                         CTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCG                            >  SRR19973077.1563394/1‑150 (MQ=60)
                                                                                                                         CTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCG                            >  SRR19973077.1563405/1‑150 (MQ=60)
                                                                                                                          TGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGA                           >  SRR19973077.4451681/1‑150 (MQ=60)
                                                                                                                          TGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGA                           >  SRR19973077.2441182/1‑150 (MQ=60)
                                                                                                                          TGATTGTGGAAAAAGGATGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGTCGCGAGAGA                           >  SRR19973077.1709016/1‑150 (MQ=60)
                                                                                                                            ATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGG                         <  SRR19973077.3126624/150‑1 (MQ=60)
                                                                                                                             TTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGG                        >  SRR19973077.1605968/1‑150 (MQ=60)
                                                                                                                              TGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGT                       <  SRR19973077.3156583/150‑1 (MQ=60)
                                                                                                                              TGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGT                       <  SRR19973077.1849537/150‑1 (MQ=60)
                                                                                                                               GTGGAAATAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTA                      <  SRR19973077.3304833/150‑1 (MQ=60)
                                                                                                                                TGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAA                     <  SRR19973077.3968962/150‑1 (MQ=60)
                                                                                                                                TGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAA                     <  SRR19973077.2939002/150‑1 (MQ=60)
                                                                                                                                TGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAA                     <  SRR19973077.2938964/150‑1 (MQ=60)
                                                                                                                                TGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAA                     <  SRR19973077.2938868/150‑1 (MQ=60)
                                                                                                                                 GGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAA                    <  SRR19973077.3649899/150‑1 (MQ=60)
                                                                                                                                  GAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAA                   >  SRR19973077.4999471/1‑150 (MQ=60)
                                                                                                                                        GGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCG             <  SRR19973077.3644664/150‑1 (MQ=60)
                                                                                                                                         GCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGT            >  SRR19973077.4167311/1‑150 (MQ=60)
                                                                                                                                         GCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGT            >  SRR19973077.4167150/1‑150 (MQ=60)
                                                                                                                                           TGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAA          >  SRR19973077.2344365/1‑150 (MQ=60)
                                                                                                                                            GGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAA         >  SRR19973077.1880192/1‑150 (MQ=60)
                                                                                                                                            GGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAA         >  SRR19973077.1880185/1‑150 (MQ=60)
                                                                                                                                             GCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAA        <  SRR19973077.2542345/150‑1 (MQ=60)
                                                                                                                                              CTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAA       >  SRR19973077.1124696/1‑150 (MQ=60)
                                                                                                                                                GGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGA     <  SRR19973077.944174/150‑1 (MQ=60)
                                                                                                                                                  ATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACA   <  SRR19973077.1217756/150‑1 (MQ=60)
                                                                                                                                                   TATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAG  >  SRR19973077.54662/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
AGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAG  >  CP009273/473991‑474287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑