Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 474,138 G→T 100% intergenic (‑65/+99) ylaB ← / ← ylaC putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase/DUF1449 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273474,1380GT100.0% 605.4 / ‑6.7 190intergenic (‑65/+99)ylaB/ylaCputative membrane‑anchored cyclic‑di‑GMP phosphodiesterase/DUF1449 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (84/105);  total (85/105)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 194 total aligned reads displayed.

CCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAA  >  CP009273/473993‑474281
                                                                                                                                                 |                                                                                                                                               
ccagATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGatat                                                                                                                                               <  2:596446/148‑1 (MQ=255)
 cagATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAtata                                                                                                                                              <  2:890590/148‑1 (MQ=255)
   gATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAag                                                                                                                                            >  2:3375097/1‑148 (MQ=255)
   gATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCGGGATGTAag                                                                                                                                            >  1:544900/1‑148 (MQ=255)
    aTGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAagg                                                                                                                                           <  1:28391/148‑1 (MQ=255)
     tGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAagga                                                                                                                                          >  1:2538384/1‑148 (MQ=255)
      gCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAaggag                                                                                                                                         >  1:743285/1‑148 (MQ=255)
      gCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAaggag                                                                                                                                         <  1:3308189/148‑1 (MQ=255)
      gCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAaggag                                                                                                                                         >  1:310779/1‑148 (MQ=255)
      gCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAaggag                                                                                                                                         >  1:2749978/1‑148 (MQ=255)
       cTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAaggagg                                                                                                                                        <  2:3295048/148‑1 (MQ=255)
         tAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTa                                                                                                                                      >  1:1053615/1‑148 (MQ=255)
         tAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTa                                                                                                                                      >  1:2806777/1‑148 (MQ=255)
          aaGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTag                                                                                                                                     <  1:277316/148‑1 (MQ=255)
               gACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCATCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTg                                                                                                                                <  2:125301/148‑1 (MQ=255)
                 cAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGt                                                                                                                              <  2:2116473/148‑1 (MQ=255)
                      agcaATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTc                                                                                                                         <  1:1968966/148‑1 (MQ=255)
                        caATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCAt                                                                                                                       >  1:2201381/1‑148 (MQ=255)
                          aTACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGa                                                                                                                     >  1:659464/1‑148 (MQ=255)
                                aaaGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGAt                                                                                                               <  1:430244/148‑1 (MQ=255)
                                aaaGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGAt                                                                                                               >  2:442097/1‑148 (MQ=255)
                                aaaGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGAt                                                                                                               >  2:3422678/1‑148 (MQ=255)
                                 aaGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATg                                                                                                              <  2:2015116/148‑1 (MQ=255)
                                 aaGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATg                                                                                                              <  2:3144676/148‑1 (MQ=255)
                                  aGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATgg                                                                                                             >  2:260403/1‑148 (MQ=255)
                                   gAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATggg                                                                                                            <  1:2965452/148‑1 (MQ=255)
                                   gAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCGTCTTAACACAGTGGTGGGAAAGTATATCGGATGGTGGAAAAAGGCTGGGGGGAGCTCAGGAGGGCGAGGTGGTCTTTCCTGACGTGATggg                                                                                                            >  2:1957178/1‑148 (MQ=255)
                                    aaTCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGt                                                                                                           >  2:2497559/1‑148 (MQ=255)
                                     aTCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTg                                                                                                          >  2:1005107/1‑148 (MQ=255)
                                        aGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGaa                                                                                                       >  1:180452/1‑148 (MQ=255)
                                         gTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGaaa                                                                                                      <  1:715558/148‑1 (MQ=255)
                                         gTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGaaa                                                                                                      <  2:592665/148‑1 (MQ=255)
                                          tACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAAc                                                                                                     >  1:1311179/1‑148 (MQ=255)
                                           aCTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACg                                                                                                    <  2:3315390/148‑1 (MQ=255)
                                            cTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGa                                                                                                   <  1:462868/148‑1 (MQ=255)
                                              cccGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGACACGATc                                                                                                 >  1:1458500/1‑148 (MQ=255)
                                              cccGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATc                                                                                                 >  2:2752967/1‑148 (MQ=255)
                                               ccGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCa                                                                                                <  1:3011215/148‑1 (MQ=255)
                                                 gAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCAtt                                                                                              <  2:953571/148‑1 (MQ=255)
                                                   aaTAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGt                                                                                            >  1:1586358/1‑148 (MQ=255)
                                                   aaTAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGt                                                                                            >  2:2105824/1‑148 (MQ=255)
                                                    aTAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTc                                                                                           >  2:2404358/1‑148 (MQ=255)
                                                     tacggCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCa                                                                                          <  2:1639920/145‑1 (MQ=255)
                                                       aGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCAtt                                                                                        <  2:2013011/148‑1 (MQ=255)
                                                       aGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCAtt                                                                                        <  2:2081782/148‑1 (MQ=255)
                                                           cGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGAc                                                                                    <  2:3308948/148‑1 (MQ=255)
                                                             aCCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCa                                                                                  <  1:3375097/148‑1 (MQ=255)
                                                                    gTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCag                                                                           <  1:756171/148‑1 (MQ=255)
                                                                     tCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCaga                                                                          >  2:2415506/1‑148 (MQ=255)
                                                                      cGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCagag                                                                         <  2:3096234/148‑1 (MQ=255)
                                                                       gtgtTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGc                                                                        <  2:3209672/148‑1 (MQ=255)
                                                                        tgtTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTTGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCt                                                                       <  2:135223/148‑1 (MQ=255)
                                                                         gtTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATCTCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCtt                                                                      <  2:1517614/148‑1 (MQ=255)
                                                                              cACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGc                                                                 <  1:2578138/148‑1 (MQ=255)
                                                                               aCCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGcc                                                                <  1:2873764/148‑1 (MQ=255)
                                                                                ccAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCt                                                               <  2:1912884/148‑1 (MQ=255)
                                                                                    cATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGTAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTa                                                           <  2:2260077/148‑1 (MQ=255)
                                                                                    cATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTa                                                           >  2:293334/1‑148 (MQ=255)
                                                                                    cATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTa                                                           >  1:1050400/1‑148 (MQ=255)
                                                                                        tCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTa                                                       >  1:2068452/1‑148 (MQ=255)
                                                                                        tCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGATGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTa                                                       >  2:2197536/1‑148 (MQ=255)
                                                                                          cTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAAc                                                     <  2:2690412/148‑1 (MQ=255)
                                                                                          cTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAAc                                                     >  2:2627051/1‑148 (MQ=255)
                                                                                          cTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAAc                                                     <  2:400807/148‑1 (MQ=255)
                                                                                           ttattaACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAAcc                                                    >  2:575423/1‑148 (MQ=255)
                                                                                                aaCACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAAc                                               <  1:116139/148‑1 (MQ=255)
                                                                                                  cacaGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACtt                                             >  2:1023982/1‑148 (MQ=255)
                                                                                                  cacaGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACtt                                             >  1:2285716/1‑148 (MQ=255)
                                                                                                  cacaGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACtt                                             >  1:2285712/1‑148 (MQ=255)
                                                                                                    caGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTaa                                           <  2:17684/148‑1 (MQ=255)
                                                                                                    caGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTaa                                           >  1:2547363/1‑148 (MQ=255)
                                                                                                    caGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTaa                                           >  1:737207/1‑148 (MQ=255)
                                                                                                    caGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTaa                                           <  2:1897952/148‑1 (MQ=255)
                                                                                                    caGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTaa                                           >  1:788947/1‑148 (MQ=255)
                                                                                                    caGTGTAGGGAAAGTAAATCTGATTGTGGAAAAAGGCTGGCTGGATATAATGAGGAAGAGCTGGTAATTCATGAAGTGATGGGTGGAAACGATAATTGTCATTTGACCAGGCGAAGAGCTATACGACTGTTATGTAAACCTAACTTaa                                           >  1:1193558/1‑148 (MQ=255)
                                                                                                     cgtgtAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAg                                          <  1:3351511/147‑1 (MQ=255)
                                                                                                     aGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAg                                          >  1:2262278/1‑148 (MQ=255)
                                                                                                      gtgtAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGa                                         <  2:3489701/148‑1 (MQ=255)
                                                                                                       tgtAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGAt                                        >  1:426312/1‑148 (MQ=255)
                                                                                                       tgtAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGAt                                        <  2:1422773/148‑1 (MQ=255)
                                                                                                           gggAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTg                                    <  2:2457132/148‑1 (MQ=255)
                                                                                                           gggAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTg                                    <  1:1419638/148‑1 (MQ=255)
                                                                                                             gAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGAt                                  <  1:838714/148‑1 (MQ=255)
                                                                                                               aaGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTc                                <  1:3502262/148‑1 (MQ=255)
                                                                                                                 gTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCgg                              >  2:2279813/1‑148 (MQ=255)
                                                                                                                 gTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCgg                              >  1:2226796/1‑148 (MQ=255)
                                                                                                                   atatCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGgcg                            >  2:1252642/1‑148 (MQ=255)
                                                                                                                   atatCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGgcg                            >  2:446873/1‑148 (MQ=255)
                                                                                                                     atCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGcgcg                          <  1:2075490/148‑1 (MQ=255)
                                                                                                                      tCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCgcga                         <  2:368069/148‑1 (MQ=255)
                                                                                                                           ttGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGcgagcgag                    <  1:1316079/148‑1 (MQ=255)
                                                                                                                             gtgGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTCAGATGTTGATTCGGCGCGAGCGAggg                  >  2:1812850/1‑148 (MQ=255)
                                                                                                                                 aaaaaGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCTTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTaaa              >  1:2894835/1‑148 (MQ=255)
                                                                                                                                 aaaaaGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTaaa              >  1:397657/1‑148 (MQ=255)
                                                                                                                                  aaaaGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTaaaa             <  2:341390/148‑1 (MQ=255)
                                                                                                                                  aaaaGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTaaaa             <  1:288599/148‑1 (MQ=255)
                                                                                                                                      ggctggctGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAatat         <  1:2627236/148‑1 (MQ=255)
                                                                                                                                            ctggATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTaaa   <  2:2862016/148‑1 (MQ=255)
                                                                                                                                            ctggATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTaaa   <  2:2380653/148‑1 (MQ=255)
                                                                                                                                             tggATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTaaaa  <  2:659464/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAA  >  CP009273/473993‑474281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAG  >  CP009273/473991‑474287
                                                                                                                                                   |                                                                                                                                                     
AGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATAT                                                                                                                                                     <  SRR19973065.3083418/150‑1 (MQ=60)
AGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATAT                                                                                                                                                     <  SRR19973065.2579939/150‑1 (MQ=60)
AGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATAT                                                                                                                                                     <  SRR19973065.2109959/150‑1 (MQ=60)
   CAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATANG                                                                                                                                                  <  SRR19973065.698065/150‑1 (MQ=60)
     GATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGA                                                                                                                                                >  SRR19973065.2554313/1‑150 (MQ=60)
     GATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGA                                                                                                                                                >  SRR19973065.2554297/1‑150 (MQ=60)
      ATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAG                                                                                                                                               >  SRR19973065.748264/1‑150 (MQ=60)
      ATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAG                                                                                                                                               >  SRR19973065.312862/1‑150 (MQ=60)
      ATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAG                                                                                                                                               <  SRR19973065.28852/150‑1 (MQ=60)
      ATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAG                                                                                                                                               <  SRR19973065.28758/150‑1 (MQ=60)
      ATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAG                                                                                                                                               >  SRR19973065.2767092/1‑150 (MQ=60)
      ATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAG                                                                                                                                               <  SRR19973065.2139509/150‑1 (MQ=60)
        GCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGT                                                                                                                                             <  SRR19973065.3328352/150‑1 (MQ=60)
        GCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGT                                                                                                                                             <  SRR19973065.2016039/150‑1 (MQ=60)
         CTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTA                                                                                                                                            >  SRR19973065.1060601/1‑150 (MQ=60)
         CTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTA                                                                                                                                            >  SRR19973065.2824146/1‑150 (MQ=60)
            AAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAG                                                                                                                                         <  SRR19973065.769234/150‑1 (MQ=60)
            AAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAG                                                                                                                                         <  SRR19973065.279169/150‑1 (MQ=60)
                    AGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATT                                                                                                                                 <  SRR19973065.1615812/150‑1 (MQ=60)
                     GGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTT                                                                                                                                <  SRR19973065.562114/150‑1 (MQ=60)
                        AGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCAT                                                                                                                             <  SRR19973065.1981806/150‑1 (MQ=60)
                        AGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCAT                                                                                                                             >  SRR19973065.1316190/1‑150 (MQ=60)
                        AGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCAT                                                                                                                             >  SRR19973065.2215477/1‑150 (MQ=60)
                        AGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCAT                                                                                                                             >  SRR19973065.2215519/1‑150 (MQ=60)
                          CAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGA                                                                                                                           >  SRR19973065.663824/1‑150 (MQ=60)
                          CAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGA                                                                                                                           <  SRR19973065.48054/150‑1 (MQ=60)
                             TACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGT                                                                                                                        <  SRR19973065.926683/150‑1 (MQ=60)
                                  AAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGG                                                                                                                   <  SRR19973065.433230/150‑1 (MQ=60)
                                  AAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGG                                                                                                                   <  SRR19973065.432781/150‑1 (MQ=60)
                                  AAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGG                                                                                                                   <  SRR19973065.433630/150‑1 (MQ=60)
                                   AAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGG                                                                                                                  >  SRR19973065.2852809/1‑150 (MQ=60)
                                    AGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGT                                                                                                                 <  SRR19973065.2425867/150‑1 (MQ=60)
                                     GAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTG                                                                                                                <  SRR19973065.2983580/150‑1 (MQ=60)
                                     GAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTG                                                                                                                >  SRR19973065.2820553/1‑150 (MQ=60)
                                        TCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAA                                                                                                             >  SRR19973065.181880/1‑150 (MQ=60)
                                          AGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAAC                                                                                                           >  SRR19973065.1319838/1‑150 (MQ=60)
                                           GTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACG                                                                                                          <  SRR19973065.720354/150‑1 (MQ=60)
                                           GTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACG                                                                                                          <  SRR19973065.720342/150‑1 (MQ=60)
                                              CTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGACACGATC                                                                                                       >  SRR19973065.1467882/1‑150 (MQ=60)
                                              CTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATC                                                                                                       <  SRR19973065.466065/150‑1 (MQ=60)
                                                 CCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATT                                                                                                    <  SRR19973065.1363392/150‑1 (MQ=60)
                                                 CCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATT                                                                                                    <  SRR19973065.3029656/150‑1 (MQ=60)
                                                   GAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGT                                                                                                  >  SRR19973065.1596942/1‑150 (MQ=60)
                                                    AAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTC                                                                                                 >  SRR19973065.1594020/1‑150 (MQ=60)
                                                         AGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGAGTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTG                                                                                            >  SRR19973065.1122067/1‑150 (MQ=60)
                                                              GACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAG                                                                                       <  SRR19973065.2259401/150‑1 (MQ=60)
                                                               ACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGG                                                                                      <  SRR19973065.3395739/150‑1 (MQ=60)
                                                                      GTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAG                                                                               <  SRR19973065.761212/150‑1 (MQ=60)
                                                                           GTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTA                                                                          >  SRR19973065.1324411/1‑150 (MQ=60)
                                                                                CACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCT                                                                     <  SRR19973065.2594216/150‑1 (MQ=60)
                                                                                 ACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTG                                                                    <  SRR19973065.2891373/150‑1 (MQ=60)
                                                                                    AGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTA                                                                 <  SRR19973065.421698/150‑1 (MQ=60)
                                                                                    AGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTA                                                                 >  SRR19973065.1057356/1‑150 (MQ=60)
                                                                                        TATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTA                                                             >  SRR19973065.2081630/1‑150 (MQ=60)
                                                                                            CTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCC                                                         <  SRR19973065.2895918/150‑1 (MQ=60)
                                                                                                 TAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACT                                                    >  SRR19973065.750546/1‑150 (MQ=60)
                                                                                                  AACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTT                                                   >  SRR19973065.2300120/1‑150 (MQ=60)
                                                                                                  AACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTT                                                   >  SRR19973065.2300124/1‑150 (MQ=60)
                                                                                                  AACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTT                                                   <  SRR19973065.117162/150‑1 (MQ=60)
                                                                                                    CACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAA                                                 >  SRR19973065.742136/1‑150 (MQ=60)
                                                                                                    CACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAA                                                 >  SRR19973065.744209/1‑150 (MQ=60)
                                                                                                    CACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAA                                                 >  SRR19973065.2563314/1‑150 (MQ=60)
                                                                                                    CACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAA                                                 >  SRR19973065.794176/1‑150 (MQ=60)
                                                                                                    CACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAA                                                 >  SRR19973065.2563344/1‑150 (MQ=60)
                                                                                                    CACAGTGTAGGGAAAGTAAATCTGATTGTGGAAAAAGGCTGGCTGGATATAATGAGGAAGAGCTGGTAATTCATGAAGTGATGGGTGGAAACGATAATTGTCATTTGACCAGGCGAAGAGCTATACGACTGTTATGTAAACCTAACTTAA                                                 >  SRR19973065.1201141/1‑150 (MQ=60)
                                                                                                     ACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAG                                                >  SRR19973065.2276581/1‑150 (MQ=60)
                                                                                                     ACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAG                                                >  SRR19973065.2277183/1‑150 (MQ=60)
                                                                                                      CAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGA                                               >  SRR19973065.1453577/1‑150 (MQ=60)
                                                                                                       CGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGAT                                              <  SRR19973065.3371991/150‑1 (MQ=60)
                                                                                                       AGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGAT                                              >  SRR19973065.3490779/1‑150 (MQ=60)
                                                                                                       AGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGAT                                              >  SRR19973065.429277/1‑150 (MQ=60)
                                                                                                       AGTGTAGGGAAAGTATAGCGGAGTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGAT                                              <  SRR19973065.2716523/150‑1 (MQ=60)
                                                                                                             GGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGAT                                        <  SRR19973065.1428905/150‑1 (MQ=60)
                                                                                                              GGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATT                                       <  SRR19973065.2770092/150‑1 (MQ=60)
                                                                                                               GAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTC                                      <  SRR19973065.844176/150‑1 (MQ=60)
                                                                                                               GAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTC                                      <  SRR19973065.844137/150‑1 (MQ=60)
                                                                                                                AAAGTATATATGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCG                                     <  SRR19973065.1922823/150‑1 (MQ=60)
                                                                                                                 AAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGG                                    <  SRR19973065.3523529/150‑1 (MQ=60)
                                                                                                                 AAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGG                                    >  SRR19973065.2240982/1‑150 (MQ=60)
                                                                                                                    TATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGC                                 >  SRR19973065.1190430/1‑150 (MQ=60)
                                                                                                                       ATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAG                              <  SRR19973065.2088753/150‑1 (MQ=60)
                                                                                                                       ATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAG                              <  SRR19973065.2087308/150‑1 (MQ=60)
                                                                                                                         CTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCG                            >  SRR19973065.3079681/1‑150 (MQ=60)
                                                                                                                            ATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGG                         >  SRR19973065.3026618/1‑150 (MQ=60)
                                                                                                                             TTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGG                        <  SRR19973065.1324785/150‑1 (MQ=60)
                                                                                                                                 GGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCTTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAA                    >  SRR19973065.2912528/1‑150 (MQ=60)
                                                                                                                                 GGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCTTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAA                    >  SRR19973065.2912511/1‑150 (MQ=60)
                                                                                                                                 GGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAA                    >  SRR19973065.400430/1‑150 (MQ=60)
                                                                                                                                 GGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAA                    >  SRR19973065.400406/1‑150 (MQ=60)
                                                                                                                                    AAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAAT                 <  SRR19973065.290706/150‑1 (MQ=60)
                                                                                                                                    AAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAAT                 <  SRR19973065.290483/150‑1 (MQ=60)
                                                                                                                                     AAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATA                >  SRR19973065.3246898/1‑150 (MQ=60)
                                                                                                                                        GGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCG             <  SRR19973065.2643487/150‑1 (MQ=60)
                                                                                                                                        GGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCG             <  SRR19973065.2643302/150‑1 (MQ=60)
                                                                                                                                          CTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTA           <  SRR19973065.3369349/150‑1 (MQ=60)
                                                                                                                                                 GATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGAC    <  SRR19973065.2843701/150‑1 (MQ=60)
                                                                                                                                                   TATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAG  >  SRR19973065.433687/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
AGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAG  >  CP009273/473991‑474287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑