Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F121 I0 R1 856 273.6 9081716 94.1% 8545894 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 474,138 G→T 89.3% intergenic (‑65/+99) ylaB ← / ← ylaC putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase/DUF1449 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273474,1380GT89.3% 705.9 / 82.1 264intergenic (‑65/+99)ylaB/ylaCputative membrane‑anchored cyclic‑di‑GMP phosphodiesterase/DUF1449 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (11/17);  new base T (116/120);  total (127/137)
Fisher's exact test for biased strand distribution p-value = 4.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.37e-01

Only 100 of 268 total aligned reads displayed.

AGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAG  >  CP009273/473991‑474283
                                                                                                                                                   |                                                                                                                                                 
agccagATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGatat                                                                                                                                                 >  1:949722/1‑150 (MQ=255)
 gccagATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAtata                                                                                                                                                <  1:3071593/150‑1 (MQ=255)
   cagATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAag                                                                                                                                              >  1:2050372/1‑150 (MQ=255)
    agATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAagg                                                                                                                                             >  2:4097105/1‑150 (MQ=255)
     gATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAagga                                                                                                                                            >  1:261779/1‑150 (MQ=255)
     gATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAagga                                                                                                                                            >  2:3680590/1‑150 (MQ=255)
     gATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAagga                                                                                                                                            >  2:1396937/1‑150 (MQ=255)
      aTGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAaggag                                                                                                                                           <  2:3251347/150‑1 (MQ=255)
       tGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAaggagg                                                                                                                                          <  2:1507059/150‑1 (MQ=255)
        gCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATACGGAGGt                                                                                                                                         >  2:1036637/1‑150 (MQ=255)
         cTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTa                                                                                                                                        <  1:972939/150‑1 (MQ=255)
         cTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTa                                                                                                                                        >  2:609859/1‑150 (MQ=255)
           tAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTaga                                                                                                                                      <  2:2801035/150‑1 (MQ=255)
           tAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTaga                                                                                                                                      <  1:4431383/150‑1 (MQ=255)
            aaGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTagag                                                                                                                                     >  1:4018920/1‑150 (MQ=255)
            aaGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTagag                                                                                                                                     <  1:3476040/150‑1 (MQ=255)
            aaGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTagag                                                                                                                                     >  1:1393010/1‑150 (MQ=255)
             aGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGc                                                                                                                                    <  1:115063/150‑1 (MQ=255)
              gCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCt                                                                                                                                   <  2:105185/150‑1 (MQ=255)
               ccGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTg                                                                                                                                  <  2:297098/150‑1 (MQ=255)
                 gACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGt                                                                                                                                >  2:1181266/1‑150 (MQ=255)
                   cAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTAt                                                                                                                              >  1:3295069/1‑150 (MQ=255)
                   cAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGAGTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTAt                                                                                                                              >  1:1015511/1‑150 (MQ=255)
                    aGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTAtt                                                                                                                             <  2:1170629/150‑1 (MQ=255)
                          caATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGa                                                                                                                       <  1:3685035/150‑1 (MQ=255)
                            aTACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAg                                                                                                                     <  2:3807856/150‑1 (MQ=255)
                             tACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGt                                                                                                                    >  1:4519558/1‑150 (MQ=255)
                              aCTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTg                                                                                                                   >  1:713070/1‑150 (MQ=255)
                              aCTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCGTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTg                                                                                                                   >  2:1847558/1‑150 (MQ=255)
                               cTGAAAGAATCAGTACTCCCGAAATAAGGTCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGa                                                                                                                  <  2:4309347/150‑1 (MQ=255)
                                 gAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATg                                                                                                                <  1:2411772/150‑1 (MQ=255)
                                      aaTCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTgg                                                                                                           <  2:75818/150‑1 (MQ=255)
                                      aaTCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTgg                                                                                                           >  2:3869920/1‑150 (MQ=255)
                                       aTCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGa                                                                                                          >  2:972470/1‑150 (MQ=255)
                                       aTCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGa                                                                                                          >  1:3928083/1‑150 (MQ=255)
                                                  cGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTg                                                                                               <  1:2489876/150‑1 (MQ=255)
                                                  cGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTg                                                                                               <  2:85592/150‑1 (MQ=255)
                                                   gAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGt                                                                                              >  2:2714094/1‑150 (MQ=255)
                                                     aaTAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCa                                                                                            >  1:3776039/1‑150 (MQ=255)
                                                        aaGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCAttt                                                                                         <  2:657559/150‑1 (MQ=255)
                                                         aGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTg                                                                                        >  2:361112/1‑150 (MQ=255)
                                                            ccGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGAcc                                                                                     <  1:2733708/150‑1 (MQ=255)
                                                             cGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCa                                                                                    >  2:3797465/1‑150 (MQ=255)
                                                             cGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCa                                                                                    >  1:3014760/1‑150 (MQ=255)
                                                                  aGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGgcgc                                                                               >  2:3439189/1‑150 (MQ=255)
                                                                   gATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCa                                                                              >  2:1647665/1‑150 (MQ=255)
                                                                     tGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCaga                                                                            >  2:3771428/1‑150 (MQ=255)
                                                                      gTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCagag                                                                           >  2:2025522/1‑150 (MQ=255)
                                                                          tgtTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCttt                                                                       <  2:344729/150‑1 (MQ=255)
                                                                          tgtTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCttt                                                                       >  1:634920/1‑150 (MQ=255)
                                                                          tgtTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCttt                                                                       >  1:4137828/1‑150 (MQ=255)
                                                                          tgtTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGACTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCttt                                                                       <  2:2524890/150‑1 (MQ=255)
                                                                           gtTCTCACCAGCCGAGCCTTAGTAACACAGTGTAGGGACAGTATATCTGATGGTGGAACAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTa                                                                      <  2:1515258/150‑1 (MQ=255)
                                                                            ttCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTAc                                                                     >  1:4526201/1‑150 (MQ=255)
                                                                                 aCCAGCATATCCTTATTAACACAGTGTAGGGATAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTg                                                                <  2:396095/150‑1 (MQ=255)
                                                                                 aCCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTg                                                                <  1:2884010/150‑1 (MQ=255)
                                                                                 aCCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTg                                                                >  2:1161131/1‑150 (MQ=255)
                                                                                 aCCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTg                                                                >  1:4271279/1‑150 (MQ=255)
                                                                                 aCCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTg                                                                <  1:2000567/150‑1 (MQ=255)
                                                                                   cAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGTTGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGt                                                              >  2:2811942/1‑150 (MQ=255)
                                                                                   cAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGt                                                              <  1:8459/150‑1 (MQ=255)
                                                                                   cAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGt                                                              <  1:878463/150‑1 (MQ=255)
                                                                                     gCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTAt                                                            >  2:3858912/1‑150 (MQ=255)
                                                                                     gCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTAt                                                            <  1:2352229/150‑1 (MQ=255)
                                                                                       atatCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGt                                                          >  1:4160387/1‑150 (MQ=255)
                                                                                         atCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTaa                                                        >  1:1675248/1‑150 (MQ=255)
                                                                                             ttattaACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAAccca                                                    <  1:2312974/150‑2 (MQ=255)
                                                                                             ttattaACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCt                                                    >  2:3185072/1‑150 (MQ=255)
                                                                                               attaACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTaa                                                  >  2:4476748/1‑150 (MQ=255)
                                                                                               attaACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTaa                                                  >  2:491269/1‑150 (MQ=255)
                                                                                                 taACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACt                                                <  1:2230763/150‑1 (MQ=255)
                                                                                                 taACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACt                                                <  2:1582909/150‑1 (MQ=255)
                                                                                                     acaGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAg                                            <  2:3998100/150‑1 (MQ=255)
                                                                                                      caGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGa                                           <  2:2405200/150‑1 (MQ=255)
                                                                                                       aGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGATGGCTGGATATAATGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGAAACCATAACTTAAGAt                                          >  2:2138103/1‑150 (MQ=255)
                                                                                                          gtAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGtt                                       <  1:2002976/150‑1 (MQ=255)
                                                                                                          gtAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGtt                                       <  2:4019850/150‑1 (MQ=255)
                                                                                                          gtAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGtt                                       <  2:3396497/150‑1 (MQ=255)
                                                                                                           tAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTg                                      >  2:1383584/1‑150 (MQ=255)
                                                                                                             gggAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGAt                                    <  1:998850/150‑1 (MQ=255)
                                                                                                               gAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTc                                  <  1:4385330/150‑1 (MQ=255)
                                                                                                                aaaGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCg                                 <  1:1847559/150‑1 (MQ=255)
                                                                                                                  aGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGgc                               >  2:2943700/1‑150 (MQ=255)
                                                                                                                  aGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTAGgc                               >  2:4254218/1‑150 (MQ=255)
                                                                                                                   gTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGgcg                              >  2:2302029/1‑150 (MQ=255)
                                                                                                                     atatCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGcgcg                            >  2:3157935/1‑150 (MQ=255)
                                                                                                                         cTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCgcgagcg                        >  1:1847117/1‑150 (MQ=255)
                                                                                                                           gATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGcgagcgag                      <  2:887502/150‑1 (MQ=255)
                                                                                                                            aTTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAgg                     <  1:288367/150‑1 (MQ=255)
                                                                                                                             ttGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAggg                    <  1:4327232/150‑1 (MQ=255)
                                                                                                                              tgtgGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGt                   <  2:4018920/150‑1 (MQ=255)
                                                                                                                              tgtgGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGt                   <  1:1750082/150‑1 (MQ=255)
                                                                                                                                tgGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTaa                 <  1:165792/150‑1 (MQ=255)
                                                                                                                                    aaaaGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAat             >  2:1673909/1‑150 (MQ=255)
                                                                                                                                     aaaGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAata            >  2:2231365/1‑150 (MQ=255)
                                                                                                                                      aaGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAatat           >  2:3044780/1‑150 (MQ=255)
                                                                                                                                      aaGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAatat           >  2:2187393/1‑150 (MQ=255)
                                                                                                                                       aGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATc          >  2:369593/1‑150 (MQ=255)
                                                                                                                                         gctggctgGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGt        <  2:3527448/150‑1 (MQ=255)
                                                                                                                                               tggATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAg  >  2:1785265/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
AGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAG  >  CP009273/473991‑474283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAG  >  CP009273/473989‑474287
                                                                                                                                                     |                                                                                                                                                     
CCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAT                                                                                                                                                       >  SRR19973080.3741213/1‑150 (MQ=60)
CCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAT                                                                                                                                                       <  SRR19973080.3087897/150‑1 (MQ=60)
CCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAT                                                                                                                                                       >  SRR19973080.666411/1‑150 (MQ=60)
  AGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATAT                                                                                                                                                     >  SRR19973080.955501/1‑150 (MQ=60)
  AGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGAT                                                                                                                                                     <  SRR19973080.948799/150‑1 (MQ=60)
   GCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATA                                                                                                                                                    <  SRR19973080.3088334/150‑1 (MQ=60)
   GCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATA                                                                                                                                                    <  SRR19973080.3088088/150‑1 (MQ=60)
     CAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAG                                                                                                                                                  >  SRR19973080.2062014/1‑150 (MQ=60)
       GATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGA                                                                                                                                                >  SRR19973080.263540/1‑150 (MQ=60)
           CTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTA                                                                                                                                            <  SRR19973080.978824/150‑1 (MQ=60)
             TAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGA                                                                                                                                          <  SRR19973080.4455453/150‑1 (MQ=60)
             TAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGA                                                                                                                                          <  SRR19973080.4454355/150‑1 (MQ=60)
              AAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAG                                                                                                                                         >  SRR19973080.1401285/1‑150 (MQ=60)
              AAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAG                                                                                                                                         <  SRR19973080.3495145/150‑1 (MQ=60)
              AAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAG                                                                                                                                         <  SRR19973080.3495382/150‑1 (MQ=60)
              AAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAG                                                                                                                                         >  SRR19973080.4040959/1‑150 (MQ=60)
               AGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGC                                                                                                                                        <  SRR19973080.116055/150‑1 (MQ=60)
                  CGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGG                                                                                                                                     <  SRR19973080.4193934/150‑1 (MQ=60)
                     CAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTAT                                                                                                                                  >  SRR19973080.3313124/1‑150 (MQ=60)
                     CAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTAT                                                                                                                                  >  SRR19973080.1000993/1‑150 (MQ=60)
                     CAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGAGTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTAT                                                                                                                                  >  SRR19973080.1021573/1‑150 (MQ=60)
                       GGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTT                                                                                                                                <  SRR19973080.740111/150‑1 (MQ=60)
                         CAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCA                                                                                                                              <  SRR19973080.2204395/150‑1 (MQ=60)
                            CAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGA                                                                                                                           <  SRR19973080.3705298/150‑1 (MQ=60)
                               TACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGT                                                                                                                        >  SRR19973080.1819373/1‑150 (MQ=60)
                               TACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGT                                                                                                                        >  SRR19973080.4543921/1‑150 (MQ=60)
                               TACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGT                                                                                                                        >  SRR19973080.4543937/1‑150 (MQ=60)
                                ACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTG                                                                                                                       >  SRR19973080.2989903/1‑150 (MQ=60)
                                ACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTG                                                                                                                       >  SRR19973080.717036/1‑150 (MQ=60)
                                ACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTG                                                                                                                       >  SRR19973080.717199/1‑150 (MQ=60)
                                   GAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATG                                                                                                                    <  SRR19973080.2425161/150‑1 (MQ=60)
                                   GAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATG                                                                                                                    <  SRR19973080.3882123/150‑1 (MQ=60)
                                    AAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGG                                                                                                                   <  SRR19973080.2699094/150‑1 (MQ=60)
                                     AAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGG                                                                                                                  >  SRR19973080.4174092/1‑150 (MQ=60)
                                        AATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGG                                                                                                               >  SRR19973080.4368556/1‑150 (MQ=60)
                                         ATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGA                                                                                                              >  SRR19973080.3949769/1‑150 (MQ=60)
                                            AGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAAC                                                                                                           <  SRR19973080.2288058/150‑1 (MQ=60)
                                              TACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGA                                                                                                         <  SRR19973080.1476981/150‑1 (MQ=60)
                                                CTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATC                                                                                                       >  SRR19973080.989365/1‑150 (MQ=60)
                                                    CGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTG                                                                                                   <  SRR19973080.3132392/150‑1 (MQ=60)
                                                    CGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTG                                                                                                   >  SRR19973080.1126465/1‑150 (MQ=60)
                                                    CGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTG                                                                                                   <  SRR19973080.2503624/150‑1 (MQ=60)
                                                    CGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTG                                                                                                   <  SRR19973080.856086/150‑1 (MQ=60)
                                                    CGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTCATGGGTGGAAACGATCATTG                                                                                                   >  SRR19973080.3424587/1‑150 (MQ=60)
                                                       AATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCA                                                                                                >  SRR19973080.3796567/1‑150 (MQ=60)
                                                       AATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCA                                                                                                >  SRR19973080.501094/1‑150 (MQ=60)
                                                        ATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCAT                                                                                               <  SRR19973080.2372575/150‑1 (MQ=60)
                                                         TAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATT                                                                                              >  SRR19973080.300384/1‑150 (MQ=60)
                                                             GCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGAC                                                                                          <  SRR19973080.2277707/150‑1 (MQ=60)
                                                              CCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACC                                                                                         <  SRR19973080.2748738/150‑1 (MQ=60)
                                                               CGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATTAAGTGATGGGTGGAAACGATCATTGTCATTTGACCA                                                                                        >  SRR19973080.959203/1‑150 (MQ=60)
                                                               CGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCA                                                                                        >  SRR19973080.3030959/1‑150 (MQ=60)
                                                               CGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCA                                                                                        >  SRR19973080.3032780/1‑150 (MQ=60)
                                                                 ACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGG                                                                                      >  SRR19973080.2418539/1‑150 (MQ=60)
                                                                            TGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTT                                                                           >  SRR19973080.638788/1‑150 (MQ=60)
                                                                            TGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTT                                                                           >  SRR19973080.4160216/1‑150 (MQ=60)
                                                                            TGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTT                                                                           <  SRR19973080.2847941/150‑1 (MQ=60)
                                                                              TTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTAC                                                                         <  SRR19973080.926125/150‑1 (MQ=60)
                                                                              TTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTAC                                                                         >  SRR19973080.4550581/1‑150 (MQ=60)
                                                                                  CACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCT                                                                     <  SRR19973080.4211421/150‑1 (MQ=60)
                                                                                  CACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCT                                                                     <  SRR19973080.4042277/150‑1 (MQ=60)
                                                                                   ACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTG                                                                    <  SRR19973080.2086306/150‑1 (MQ=60)
                                                                                   ACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTG                                                                    >  SRR19973080.4294596/1‑150 (MQ=60)
                                                                                   ACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTG                                                                    <  SRR19973080.2899786/150‑1 (MQ=60)
                                                                                   ACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTG                                                                    <  SRR19973080.2011948/150‑1 (MQ=60)
                                                                                   ACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTG                                                                    <  SRR19973080.2011945/150‑1 (MQ=60)
                                                                                   ACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTG                                                                    <  SRR19973080.2011940/150‑1 (MQ=60)
                                                                                   ACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTG                                                                    >  SRR19973080.2004454/1‑150 (MQ=60)
                                                                                   ACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTG                                                                    >  SRR19973080.2004419/1‑150 (MQ=60)
                                                                                     CAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGT                                                                  <  SRR19973080.8555/150‑1 (MQ=60)
                                                                                     CAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGT                                                                  >  SRR19973080.1736209/1‑150 (MQ=60)
                                                                                     CAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGT                                                                  >  SRR19973080.4026815/1‑150 (MQ=60)
                                                                                     CAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGT                                                                  <  SRR19973080.883829/150‑1 (MQ=60)
                                                                                     CAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGT                                                                  <  SRR19973080.883838/150‑1 (MQ=60)
                                                                                     CAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGT                                                                  <  SRR19973080.3660918/150‑1 (MQ=60)
                                                                                       GCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTAT                                                                <  SRR19973080.2365302/150‑1 (MQ=60)
                                                                                       GCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTAT                                                                <  SRR19973080.2365304/150‑1 (MQ=60)
                                                                                         ATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGT                                                              >  SRR19973080.4182832/1‑150 (MQ=60)
                                                                                           ATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAA                                                            >  SRR19973080.1685035/1‑150 (MQ=60)
                                                                                               TTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCA                                                        <  SRR19973080.2325799/150‑1 (MQ=60)
                                                                                                TATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTA                                                       >  SRR19973080.138225/1‑150 (MQ=60)
                                                                                                 ATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAA                                                      >  SRR19973080.3773863/1‑150 (MQ=60)
                                                                                                   TAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACT                                                    <  SRR19973080.4527043/150‑1 (MQ=60)
                                                                                                   TAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACT                                                    <  SRR19973080.2243047/150‑1 (MQ=60)
                                                                                                    AACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTT                                                   <  SRR19973080.4030937/150‑1 (MQ=60)
                                                                                                        CAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGA                                               <  SRR19973080.3414003/150‑1 (MQ=60)
                                                                                                         AGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGAT                                              <  SRR19973080.1245142/150‑1 (MQ=60)
                                                                                                         AGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGAT                                              <  SRR19973080.2475064/150‑1 (MQ=60)
                                                                                                         AGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGAT                                              <  SRR19973080.2474061/150‑1 (MQ=60)
                                                                                                            GTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTT                                           <  SRR19973080.2014365/150‑1 (MQ=60)
                                                                                                             TAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTG                                          >  SRR19973080.4448823/1‑150 (MQ=60)
                                                                                                               GGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGAT                                        <  SRR19973080.1004841/150‑1 (MQ=60)
                                                                                                                 GAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTC                                      <  SRR19973080.4409226/150‑1 (MQ=60)
                                                                                                                  AAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCG                                     <  SRR19973080.1857943/150‑1 (MQ=60)
                                                                                                                  AAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCG                                     <  SRR19973080.1857944/150‑1 (MQ=60)
                                                                                                                   AAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGG                                    <  SRR19973080.2743673/150‑1 (MQ=60)
                                                                                                                           CTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCG                            >  SRR19973080.1857429/1‑150 (MQ=60)
                                                                                                                           CTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCG                            >  SRR19973080.1857502/1‑150 (MQ=60)
                                                                                                                              ATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGG                         <  SRR19973080.290230/150‑1 (MQ=60)
                                                                                                                              ATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGG                         <  SRR19973080.290260/150‑1 (MQ=60)
                                                                                                                               TTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGG                        <  SRR19973080.4350867/150‑1 (MQ=60)
                                                                                                                                TGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGT                       <  SRR19973080.1760183/150‑1 (MQ=60)
                                                                                                                                TGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGT                       <  SRR19973080.4500976/150‑1 (MQ=60)
                                                                                                                                  TGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAA                     <  SRR19973080.167108/150‑1 (MQ=60)
                                                                                                                                    GAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAA                   <  SRR19973080.606426/150‑1 (MQ=60)
                                                                                                                                    GAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAA                   <  SRR19973080.1670697/150‑1 (MQ=60)
                                                                                                                                      AAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAAT                 >  SRR19973080.1467706/1‑150 (MQ=60)
                                                                                                                                      AAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAAT                 >  SRR19973080.3761618/1‑150 (MQ=60)
                                                                                                                                        AAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATAT               <  SRR19973080.2786167/150‑1 (MQ=60)
                                                                                                                                         AGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATC              <  SRR19973080.640722/150‑1 (MQ=60)
                                                                                                                                         AGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATC              <  SRR19973080.2527123/150‑1 (MQ=60)
                                                                                                                                         AGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATC              <  SRR19973080.4092770/150‑1 (MQ=60)
                                                                                                                                           GCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGT            <  SRR19973080.1745424/150‑1 (MQ=60)
                                                                                                                                             TGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAA          <  SRR19973080.4317763/150‑1 (MQ=60)
                                                                                                                                             TGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAA          <  SRR19973080.4317575/150‑1 (MQ=60)
                                                                                                                                               GCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAA        <  SRR19973080.2037037/150‑1 (MQ=60)
                                                                                                                                                    ATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACA   >  SRR19973080.3303200/1‑150 (MQ=60)
                                                                                                                                                     TATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAG  <  SRR19973080.1703987/150‑1 (MQ=60)
                                                                                                                                                     TATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAG  >  SRR19973080.4073551/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
CCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAG  >  CP009273/473989‑474287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑