Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 474,138 G→T 100% intergenic (‑65/+99) ylaB ← / ← ylaC putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase/DUF1449 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273474,1380GT100.0% 713.9 / NA 219intergenic (‑65/+99)ylaB/ylaCputative membrane‑anchored cyclic‑di‑GMP phosphodiesterase/DUF1449 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (114/105);  total (114/105)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 223 total aligned reads displayed.

CCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAA  >  CP009273/473993‑474282
                                                                                                                                                 |                                                                                                                                                
 cagATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAtata                                                                                                                                               <  1:1564042/148‑1 (MQ=255)
     tGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAagga                                                                                                                                           <  1:842455/148‑1 (MQ=255)
       cTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATCTAaggagg                                                                                                                                         >  1:1963129/1‑148 (MQ=255)
         tAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTa                                                                                                                                       >  2:2291497/1‑148 (MQ=255)
         tAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTa                                                                                                                                       <  1:654509/148‑1 (MQ=255)
          aaGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTag                                                                                                                                      <  2:723071/148‑1 (MQ=255)
           aGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGCGGTaga                                                                                                                                     >  2:3074522/1‑148 (MQ=255)
           aGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTaga                                                                                                                                     <  2:2014972/148‑1 (MQ=255)
            gCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTagag                                                                                                                                    >  2:2315250/1‑148 (MQ=255)
            gCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTagag                                                                                                                                    <  2:791528/148‑1 (MQ=255)
             ccGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGc                                                                                                                                   <  2:1958049/148‑1 (MQ=255)
               gACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTg                                                                                                                                 <  1:1298390/148‑1 (MQ=255)
                aCAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTgg                                                                                                                                >  2:579145/1‑148 (MQ=255)
                   ggCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTAt                                                                                                                             >  1:747496/1‑148 (MQ=255)
                      agcaATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTc                                                                                                                          <  1:2902675/148‑1 (MQ=255)
                        caATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCAt                                                                                                                        >  1:947075/1‑148 (MQ=255)
                          aTACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGa                                                                                                                      >  1:2451914/1‑148 (MQ=255)
                              tGATAGTATCTGTATTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTg                                                                                                                  <  2:1111799/148‑1 (MQ=255)
                              tGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTg                                                                                                                  <  1:3409171/148‑1 (MQ=255)
                               gAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGa                                                                                                                 >  2:3347940/1‑148 (MQ=255)
                                aaaGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGAt                                                                                                                >  1:3446407/1‑148 (MQ=255)
                                aaaGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGAATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAATTGAt                                                                                                                >  2:1587096/1‑148 (MQ=255)
                                   gAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATggg                                                                                                             >  1:812612/1‑148 (MQ=255)
                                   gAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATggg                                                                                                             >  1:812172/1‑148 (MQ=255)
                                   gAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATggg                                                                                                             >  1:1800471/1‑148 (MQ=255)
                                      tCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTgg                                                                                                          >  1:2842484/1‑148 (MQ=255)
                                        aGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGaa                                                                                                        <  2:1835442/148‑1 (MQ=255)
                                         gTACTCCCGAAATAAGGCCGACCATATGTCGTGTTCTCACCATCATATCCTTATTAACACAGTGTAGGGTAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGaaa                                                                                                       <  2:3435136/148‑1 (MQ=255)
                                          tACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAAc                                                                                                      >  1:3040679/1‑148 (MQ=255)
                                          tACTCCCGAAATAAGGCCGACCAGATGTCGTCTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAAc                                                                                                      >  2:3437118/1‑148 (MQ=255)
                                           aCTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACg                                                                                                     <  2:2034995/148‑1 (MQ=255)
                                            cTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGa                                                                                                    <  2:382995/148‑1 (MQ=255)
                                              cccGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATc                                                                                                  <  2:1592707/148‑1 (MQ=255)
                                               ccGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCa                                                                                                 <  1:633480/148‑1 (MQ=255)
                                                cGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCAt                                                                                                <  1:1041381/148‑1 (MQ=255)
                                                    aTAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTc                                                                                            <  2:3125754/148‑1 (MQ=255)
                                                    aTAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTc                                                                                            >  2:1265645/1‑148 (MQ=255)
                                                      aaGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGTAAGTATATCTGTTTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCAt                                                                                          <  1:1055643/148‑1 (MQ=255)
                                                      aaGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCAt                                                                                          >  2:3226762/1‑148 (MQ=255)
                                                      aaGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCAt                                                                                          <  2:582147/148‑1 (MQ=255)
                                                       aGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCAtt                                                                                         >  2:1121211/1‑148 (MQ=255)
                                                       aGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCAtt                                                                                         <  1:1752661/148‑1 (MQ=255)
                                                       aGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCAtt                                                                                         >  2:652637/1‑148 (MQ=255)
                                                         gCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTg                                                                                       <  1:1451567/148‑1 (MQ=255)
                                                         gCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTg                                                                                       <  2:2859889/148‑1 (MQ=255)
                                                           cGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGAc                                                                                     <  2:1699616/148‑1 (MQ=255)
                                                             aCCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCa                                                                                   >  1:1704207/1‑148 (MQ=255)
                                                                    gTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCag                                                                            <  1:3224843/148‑1 (MQ=255)
                                                                      cGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCagag                                                                          >  2:1758200/1‑148 (MQ=255)
                                                                      cGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCagag                                                                          >  1:1623875/1‑148 (MQ=255)
                                                                      cGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCagag                                                                          >  1:1623934/1‑148 (MQ=255)
                                                                      cGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCagag                                                                          <  2:3176473/148‑1 (MQ=255)
                                                                         gtTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCtt                                                                       >  2:2420040/1‑148 (MQ=255)
                                                                           tctcACCAGTATATCCTTATTAACACCGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTa                                                                     <  2:1908177/148‑1 (MQ=255)
                                                                               aCCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGcc                                                                 <  2:1061962/148‑1 (MQ=255)
                                                                                 cAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTg                                                               >  1:400915/1‑148 (MQ=255)
                                                                                    cATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTa                                                            <  1:1736778/148‑1 (MQ=255)
                                                                                    cATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTa                                                            >  1:3553269/1‑148 (MQ=255)
                                                                                       atCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGt                                                         >  1:2515952/1‑148 (MQ=255)
                                                                                        tCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTa                                                        >  1:288586/1‑148 (MQ=255)
                                                                                          cTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAAc                                                      <  2:254427/148‑1 (MQ=255)
                                                                                            tattaACACAGTTTAGGGAATGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAAccc                                                    <  1:1824228/148‑1 (MQ=255)
                                                                                            tattaACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAAccc                                                    <  2:1394466/148‑1 (MQ=255)
                                                                                               taACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTaa                                                 <  1:260054/148‑1 (MQ=255)
                                                                                                 acacAGTGTAGGGAAAGTATATCTTATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACt                                               >  2:1932071/1‑148 (MQ=255)
                                                                                                 acacAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACt                                               >  2:886327/1‑148 (MQ=255)
                                                                                                   acaGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTa                                             >  1:933968/1‑148 (MQ=255)
                                                                                                   acaGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTa                                             <  2:448936/148‑1 (MQ=255)
                                                                                                   acaGTGTAGGGAAAGTATATCTGAGTGTGGAAAAAGGCGGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTa                                             <  2:2741492/148‑1 (MQ=255)
                                                                                                    caGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTaa                                            >  1:1725876/1‑148 (MQ=255)
                                                                                                      gtgtAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGa                                          <  2:2777128/148‑1 (MQ=255)
                                                                                                      gtgtAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGa                                          <  2:2383108/148‑1 (MQ=255)
                                                                                                       tgtAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGAt                                         >  2:1677038/1‑148 (MQ=255)
                                                                                                           gggAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTg                                     >  1:580658/1‑148 (MQ=255)
                                                                                                           gggAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTg                                     >  1:2489090/1‑148 (MQ=255)
                                                                                                           gggAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTg                                     <  2:2347197/148‑1 (MQ=255)
                                                                                                            ggAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGa                                    >  1:2141615/1‑148 (MQ=255)
                                                                                                            ggAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGa                                    >  1:2141617/1‑148 (MQ=255)
                                                                                                            ggAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGa                                    <  2:1290217/148‑1 (MQ=255)
                                                                                                             gAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGAt                                   >  2:3348152/1‑148 (MQ=255)
                                                                                                             gAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGAt                                   <  1:2401631/148‑1 (MQ=255)
                                                                                                              aaaGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGAtt                                  <  1:680205/148‑1 (MQ=255)
                                                                                                               aaGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTc                                 <  2:1135218/148‑1 (MQ=255)
                                                                                                                  tataTCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGgc                              >  1:1993312/1‑148 (MQ=255)
                                                                                                                   atatCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGgcg                             <  1:2105675/148‑1 (MQ=255)
                                                                                                                       cTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCgcgag                         >  1:922541/1‑148 (MQ=255)
                                                                                                                       cTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCgcgag                         >  1:2699221/1‑148 (MQ=255)
                                                                                                                        tGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCgcgagc                        >  1:1269248/1‑148 (MQ=255)
                                                                                                                         gATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCgcgagcg                       >  2:1290629/1‑148 (MQ=255)
                                                                                                                         gATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCgcgagcg                       >  1:664527/1‑148 (MQ=255)
                                                                                                                            tgtgGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAgg                    >  2:2593412/1‑148 (MQ=255)
                                                                                                                               ggAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTa                 >  1:2973198/1‑148 (MQ=255)
                                                                                                                                 aaaaaGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTaaa               <  2:3129271/148‑1 (MQ=255)
                                                                                                                                 aaaaaGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTaaa               <  1:1582927/148‑1 (MQ=255)
                                                                                                                                      ggctggctGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAatat          >  1:860938/1‑148 (MQ=255)
                                                                                                                                       gctggctgGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATc         >  1:3317199/1‑148 (MQ=255)
                                                                                                                                       gctggctgGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATc         >  1:1991752/1‑148 (MQ=255)
                                                                                                                                           gctggATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTaa     >  2:2769135/1‑148 (MQ=255)
                                                                                                                                             tggATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTaaaa   <  1:1885924/148‑1 (MQ=255)
                                                                                                                                              ggATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTaaaaa  >  1:2957494/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAA  >  CP009273/473993‑474282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGAC  >  CP009273/473990‑474285
                                                                                                                                                    |                                                                                                                                                   
CAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATA                                                                                                                                                    >  SRR19973067.286127/1‑150 (MQ=60)
CAGCCAGATGCTTAAGCCGACAGGCAGCAAAACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATA                                                                                                                                                    >  SRR19973067.700149/1‑150 (MQ=60)
    CAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAG                                                                                                                                                >  SRR19973067.1763152/1‑150 (MQ=60)
    CAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAG                                                                                                                                                <  SRR19973067.1574738/150‑1 (MQ=60)
    CAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAG                                                                                                                                                <  SRR19973067.1573740/150‑1 (MQ=60)
    CAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAG                                                                                                                                                <  SRR19973067.1573853/150‑1 (MQ=60)
     AGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCGTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGG                                                                                                                                               <  SRR19973067.1069770/150‑1 (MQ=60)
        TGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATCTAAGGAGG                                                                                                                                            >  SRR19973067.1976465/1‑150 (MQ=60)
        TGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGG                                                                                                                                            <  SRR19973067.848170/150‑1 (MQ=60)
            TAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGA                                                                                                                                        <  SRR19973067.658957/150‑1 (MQ=60)
            TAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGA                                                                                                                                        <  SRR19973067.659334/150‑1 (MQ=60)
            TAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGA                                                                                                                                        <  SRR19973067.2065796/150‑1 (MQ=60)
             AAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAG                                                                                                                                       <  SRR19973067.2894970/150‑1 (MQ=60)
              AGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGC                                                                                                                                      >  SRR19973067.494568/1‑150 (MQ=60)
                  GACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGT                                                                                                                                  <  SRR19973067.1307255/150‑1 (MQ=60)
                    CAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTAT                                                                                                                                >  SRR19973067.752683/1‑150 (MQ=60)
                         AGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCAT                                                                                                                           >  SRR19973067.3461715/1‑150 (MQ=60)
                         AGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCAT                                                                                                                           <  SRR19973067.2921183/150‑1 (MQ=60)
                         AGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCAT                                                                                                                           <  SRR19973067.2921172/150‑1 (MQ=60)
                         AGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCAT                                                                                                                           >  SRR19973067.953546/1‑150 (MQ=60)
                          GCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATG                                                                                                                          <  SRR19973067.235307/150‑1 (MQ=60)
                           CAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGA                                                                                                                         >  SRR19973067.2468032/1‑150 (MQ=60)
                                 TGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGAT                                                                                                                   <  SRR19973067.3430871/150‑1 (MQ=60)
                                 TGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGAT                                                                                                                   >  SRR19973067.3468338/1‑150 (MQ=60)
                                 TGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGAT                                                                                                                   >  SRR19973067.243450/1‑150 (MQ=60)
                                 TGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGAT                                                                                                                   <  SRR19973067.3266341/150‑1 (MQ=60)
                                    AAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGG                                                                                                                >  SRR19973067.817745/1‑150 (MQ=60)
                                    AAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGG                                                                                                                >  SRR19973067.818188/1‑150 (MQ=60)
                                    AAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGG                                                                                                                >  SRR19973067.818186/1‑150 (MQ=60)
                                    AAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGG                                                                                                                >  SRR19973067.1812565/1‑150 (MQ=60)
                                    AAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGG                                                                                                                >  SRR19973067.1810517/1‑150 (MQ=60)
                                    AAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGG                                                                                                                >  SRR19973067.3142255/1‑150 (MQ=60)
                                       AATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGG                                                                                                             >  SRR19973067.2860733/1‑150 (MQ=60)
                                         TCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAA                                                                                                           >  SRR19973067.1942552/1‑150 (MQ=60)
                                           AGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAAC                                                                                                         <  SRR19973067.160071/150‑1 (MQ=60)
                                           AGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAAC                                                                                                         <  SRR19973067.158017/150‑1 (MQ=60)
                                           AGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAAC                                                                                                         >  SRR19973067.3059605/1‑150 (MQ=60)
                                           AGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAAC                                                                                                         >  SRR19973067.3060047/1‑150 (MQ=60)
                                            GTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACG                                                                                                        <  SRR19973067.861407/150‑1 (MQ=60)
                                                  CCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATT                                                                                                  <  SRR19973067.638029/150‑1 (MQ=60)
                                                  CCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATT                                                                                                  <  SRR19973067.637727/150‑1 (MQ=60)
                                                   CGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTG                                                                                                 <  SRR19973067.1048541/150‑1 (MQ=60)
                                                     AAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTC                                                                                               <  SRR19973067.770808/150‑1 (MQ=60)
                                                         AAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGTAAGTATATCTGTTTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTT                                                                                           <  SRR19973067.1062881/150‑1 (MQ=60)
                                                         AAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTT                                                                                           <  SRR19973067.3428426/150‑1 (MQ=60)
                                                         AAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTT                                                                                           <  SRR19973067.1062426/150‑1 (MQ=60)
                                                          AGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGANAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTG                                                                                          >  SRR19973067.1813496/1‑150 (MQ=60)
                                                          AGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTG                                                                                          <  SRR19973067.1764604/150‑1 (MQ=60)
                                                            GCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGAC                                                                                        <  SRR19973067.1461291/150‑1 (MQ=60)
                                                             CCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACC                                                                                       >  SRR19973067.894990/1‑150 (MQ=60)
                                                             CCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACC                                                                                       <  SRR19973067.836526/150‑1 (MQ=60)
                                                              CGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCA                                                                                      >  SRR19973067.1745601/1‑150 (MQ=60)
                                                              CGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCA                                                                                      >  SRR19973067.1715903/1‑150 (MQ=60)
                                                                  CAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGACACGATCATTGTCATTTGACCAGGCG                                                                                  >  SRR19973067.171997/1‑150 (MQ=60)
                                                                  CAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCG                                                                                  >  SRR19973067.171201/1‑150 (MQ=60)
                                                                  CAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCG                                                                                  >  SRR19973067.171297/1‑150 (MQ=60)
                                                                  CAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCG                                                                                  <  SRR19973067.2330485/150‑1 (MQ=60)
                                                                    GATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCA                                                                                >  SRR19973067.2474963/1‑150 (MQ=60)
                                                                    GATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCA                                                                                <  SRR19973067.2671416/150‑1 (MQ=60)
                                                                       GTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAG                                                                             >  SRR19973067.1635024/1‑150 (MQ=60)
                                                                       GTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAG                                                                             >  SRR19973067.1635017/1‑150 (MQ=60)
                                                                       GTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAG                                                                             >  SRR19973067.1635076/1‑150 (MQ=60)
                                                                       GTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAG                                                                             <  SRR19973067.3245102/150‑1 (MQ=60)
                                                                                  ACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTG                                                                  >  SRR19973067.403713/1‑150 (MQ=60)
                                                                                    CAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGT                                                                <  SRR19973067.841803/150‑1 (MQ=60)
                                                                                     AGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTA                                                               >  SRR19973067.3575670/1‑150 (MQ=60)
                                                                                       CATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATG                                                             <  SRR19973067.1748659/150‑1 (MQ=60)
                                                                                        ATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGT                                                            >  SRR19973067.2534346/1‑150 (MQ=60)
                                                                                        ATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGT                                                            >  SRR19973067.2532446/1‑150 (MQ=60)
                                                                                        ATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGT                                                            >  SRR19973067.1431467/1‑150 (MQ=60)
                                                                                         TATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTA                                                           <  SRR19973067.582867/150‑1 (MQ=60)
                                                                                         TATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTA                                                           >  SRR19973067.290459/1‑150 (MQ=60)
                                                                                               TATTAACACAGTTTAGGGAATGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTA                                                     <  SRR19973067.1836607/150‑1 (MQ=60)
                                                                                               TATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTA                                                     <  SRR19973067.1836361/150‑1 (MQ=60)
                                                                                               TATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTA                                                     <  SRR19973067.1836625/150‑1 (MQ=60)
                                                                                               TATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTA                                                     >  SRR19973067.1289171/1‑150 (MQ=60)
                                                                                                  TAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACT                                                  <  SRR19973067.261830/150‑1 (MQ=60)
                                                                                                  TAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACT                                                  <  SRR19973067.699564/150‑1 (MQ=60)
                                                                                                    ACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTA                                                >  SRR19973067.940302/1‑150 (MQ=60)
                                                                                                    ACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTA                                                >  SRR19973067.940286/1‑150 (MQ=60)
                                                                                                     CACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAA                                               >  SRR19973067.1737704/1‑150 (MQ=60)
                                                                                                      ACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAG                                              >  SRR19973067.982714/1‑150 (MQ=60)
                                                                                                            TAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTG                                        >  SRR19973067.2505450/1‑150 (MQ=60)
                                                                                                            TAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTG                                        >  SRR19973067.584383/1‑150 (MQ=60)
                                                                                                             AGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGA                                       >  SRR19973067.2155927/1‑150 (MQ=60)
                                                                                                             AGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGA                                       >  SRR19973067.2155931/1‑150 (MQ=60)
                                                                                                             AGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGA                                       >  SRR19973067.2155932/1‑150 (MQ=60)
                                                                                                             AGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGA                                       >  SRR19973067.2155933/1‑150 (MQ=60)
                                                                                                              GGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGAT                                      <  SRR19973067.3331046/150‑1 (MQ=60)
                                                                                                                GAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTC                                    >  SRR19973067.949442/1‑150 (MQ=60)
                                                                                                                GAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTC                                    <  SRR19973067.295944/150‑1 (MQ=60)
                                                                                                                GAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTC                                    <  SRR19973067.2417305/150‑1 (MQ=60)
                                                                                                                 AAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCG                                   <  SRR19973067.684878/150‑1 (MQ=60)
                                                                                                                 AAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCG                                   <  SRR19973067.684785/150‑1 (MQ=60)
                                                                                                                   AGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGC                                 >  SRR19973067.198617/1‑150 (MQ=60)
                                                                                                                   AGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGC                                 >  SRR19973067.2006779/1‑150 (MQ=60)
                                                                                                                      ATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCG                              <  SRR19973067.2119652/150‑1 (MQ=60)
                                                                                                                        ATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAG                            >  SRR19973067.2716586/1‑150 (MQ=60)
                                                                                                                        ATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAG                            >  SRR19973067.928742/1‑150 (MQ=60)
                                                                                                                         TCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGC                           >  SRR19973067.1277881/1‑150 (MQ=60)
                                                                                                                          CTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCG                          >  SRR19973067.2682617/1‑150 (MQ=60)
                                                                                                                          CTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCG                          >  SRR19973067.669091/1‑150 (MQ=60)
                                                                                                                          CTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCG                          >  SRR19973067.669064/1‑150 (MQ=60)
                                                                                                                            GATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAG                        <  SRR19973067.390524/150‑1 (MQ=60)
                                                                                                                             ATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGG                       <  SRR19973067.606846/150‑1 (MQ=60)
                                                                                                                               TGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGT                     >  SRR19973067.2723224/1‑150 (MQ=60)
                                                                                                                                GTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTA                    >  SRR19973067.2991945/1‑150 (MQ=60)
                                                                                                                                    AAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAA                <  SRR19973067.1593767/150‑1 (MQ=60)
                                                                                                                                     AAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAAT               >  SRR19973067.253312/1‑150 (MQ=60)
                                                                                                                                       AAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATAT             >  SRR19973067.866717/1‑150 (MQ=60)
                                                                                                                                        AGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATC            >  SRR19973067.2005390/1‑150 (MQ=60)
                                                                                                                                        AGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATC            >  SRR19973067.2005314/1‑150 (MQ=60)
                                                                                                                                        AGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATC            >  SRR19973067.2005209/1‑150 (MQ=60)
                                                                                                                                        AGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATC            >  SRR19973067.3338174/1‑150 (MQ=60)
                                                                                                                                               CTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAA     >  SRR19973067.2976186/1‑150 (MQ=60)
                                                                                                                                               CTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAA     >  SRR19973067.2976286/1‑150 (MQ=60)
                                                                                                                                                TGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAG    <  SRR19973067.2932976/150‑1 (MQ=60)
                                                                                                                                                TGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAG    <  SRR19973067.1898799/150‑1 (MQ=60)
                                                                                                                                                 GGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGA   <  SRR19973067.3533297/150‑1 (MQ=60)
                                                                                                                                                  GATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGAC  <  SRR19973067.2272173/150‑1 (MQ=60)
                                                                                                                                                  GATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGAC  <  SRR19973067.2272364/150‑1 (MQ=60)
                                                                                                                                                  GATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGAC  <  SRR19973067.2789632/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                   
CAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGAC  >  CP009273/473990‑474285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑