Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F78 I0 R1 626 241.3 10389380 77.7% 8072548 149.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 474,138 G→T 88.1% intergenic (‑65/+99) ylaB ← / ← ylaC putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase/DUF1449 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273474,1380GT88.1% 624.9 / 74.3 226intergenic (‑65/+99)ylaB/ylaCputative membrane‑anchored cyclic‑di‑GMP phosphodiesterase/DUF1449 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (9/18);  new base T (93/106);  total (102/124)
Fisher's exact test for biased strand distribution p-value = 2.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01

Only 100 of 232 total aligned reads displayed.

CAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGA  >  CP009273/473990‑474284
                                                                                                                                                    |                                                                                                                                                  
 agccagATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGatat                                                                                                                                                  <  1:519178/150‑1 (MQ=255)
 agccagATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGatat                                                                                                                                                  <  1:3449241/150‑1 (MQ=255)
   ccagATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATaa                                                                                                                                                <  2:3892898/150‑1 (MQ=255)
     agATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAcgg                                                                                                                                              >  2:4930350/1‑150 (MQ=255)
        tGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAaggagg                                                                                                                                           <  1:5054701/150‑1 (MQ=255)
        tGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAaggagg                                                                                                                                           >  1:1514143/1‑150 (MQ=255)
        tGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAaggagg                                                                                                                                           >  2:2840711/1‑150 (MQ=255)
        tGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAaggagg                                                                                                                                           >  1:2946497/1‑150 (MQ=255)
         gCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGt                                                                                                                                          >  2:3749119/1‑150 (MQ=255)
          cTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTa                                                                                                                                         <  2:1103855/150‑1 (MQ=255)
          cTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTa                                                                                                                                         >  2:4864948/1‑150 (MQ=255)
          cTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTa                                                                                                                                         >  2:4242068/1‑150 (MQ=255)
          cTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTa                                                                                                                                         >  2:2808818/1‑150 (MQ=255)
           ttAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTag                                                                                                                                        <  2:4858996/150‑1 (MQ=255)
            tAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTaga                                                                                                                                       >  1:866430/1‑150 (MQ=255)
                ccGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTg                                                                                                                                   <  2:1977842/150‑1 (MQ=255)
                 cGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTgg                                                                                                                                  >  2:4825027/1‑150 (MQ=255)
                 cGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTgg                                                                                                                                  <  2:2082906/150‑1 (MQ=255)
                         agcaATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCAt                                                                                                                          <  1:4922310/150‑1 (MQ=255)
                            aaTACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGaa                                                                                                                       >  1:985313/1‑150 (MQ=255)
                             aTACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATCAGGAGGTAGAGCTGGTATTTCATGAAg                                                                                                                      >  2:4284263/1‑150 (MQ=255)
                               aCTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTg                                                                                                                    <  2:4974967/150‑1 (MQ=255)
                                cTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGa                                                                                                                   >  2:2482240/1‑150 (MQ=255)
                                 tGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGAt                                                                                                                  >  2:4727486/1‑150 (MQ=255)
                                    aaGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATggg                                                                                                               <  2:2297756/150‑1 (MQ=255)
                                      gAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTg                                                                                                             >  2:885125/1‑150 (MQ=255)
                                       aaTCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTgg                                                                                                            <  2:2601182/150‑1 (MQ=255)
                                            gTACTCCCGAAATAAGGCCGACCAGATGTCGGGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGGTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACg                                                                                                       >  2:2103041/1‑150 (MQ=255)
                                             tACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGa                                                                                                      >  2:4485990/1‑150 (MQ=255)
                                               cTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATc                                                                                                    <  2:1341181/150‑1 (MQ=255)
                                                 cccGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCAt                                                                                                  <  1:1602924/150‑1 (MQ=255)
                                                  ccGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCAtt                                                                                                 <  1:2202238/150‑1 (MQ=255)
                                                    gAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAACCGATCATTGt                                                                                               >  2:2704523/1‑150 (MQ=255)
                                                    gAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGt                                                                                               >  2:1151844/1‑150 (MQ=255)
                                                    gAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGt                                                                                               >  2:4418252/1‑150 (MQ=255)
                                                    gAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGt                                                                                               <  1:4261255/150‑1 (MQ=255)
                                                      aaTAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCATTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGTTCATTGTCa                                                                                             <  2:4447261/150‑1 (MQ=255)
                                                       aTAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCATTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGAATTTCATGAAGTGATGGGTGGAAACGATCAATGTCAt                                                                                            >  2:3377541/1‑150 (MQ=255)
                                                          aGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTg                                                                                         <  2:1132434/150‑1 (MQ=255)
                                                            gCCGACCAGATGTCGCGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGAc                                                                                       <  1:3088004/150‑1 (MQ=255)
                                                             ccGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGAcc                                                                                      <  2:1307206/150‑1 (MQ=255)
                                                              cGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCa                                                                                     >  1:4557403/1‑150 (MQ=255)
                                                               gACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGGGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAg                                                                                    <  1:2260880/150‑1 (MQ=255)
                                                               gACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAg                                                                                    <  2:1271693/150‑1 (MQ=255)
                                                               gACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAg                                                                                    <  2:2000886/150‑1 (MQ=255)
                                                                aCCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACCGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAgg                                                                                   <  2:1750164/150‑1 (MQ=255)
                                                                aCCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGACAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAgg                                                                                   >  1:2668137/1‑150 (MQ=255)
                                                                 ccAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATACGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGgc                                                                                  >  1:2039068/1‑150 (MQ=255)
                                                                 ccAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGgc                                                                                  <  1:863464/150‑1 (MQ=255)
                                                                   aGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGgcgc                                                                                >  2:400243/1‑150 (MQ=255)
                                                                     aTGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCag                                                                              <  1:4849839/150‑1 (MQ=255)
                                                                       gTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGACAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCAGTGTCATTTGACCAGGCGCagag                                                                            >  1:3927933/1‑150 (MQ=255)
                                                                        tCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGc                                                                           <  2:4012373/150‑1 (MQ=255)
                                                                        tCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGc                                                                           <  1:5095013/150‑1 (MQ=255)
                                                                          gtgtTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCtt                                                                         <  2:865574/150‑1 (MQ=255)
                                                                          gtgtTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCtt                                                                         >  1:331033/1‑150 (MQ=255)
                                                                            gtTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTa                                                                       <  1:948601/150‑1 (MQ=255)
                                                                            gtTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTa                                                                       >  2:3104936/1‑150 (MQ=255)
                                                                            gtTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTa                                                                       >  1:1107802/1‑150 (MQ=255)
                                                                                  aCCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTg                                                                 >  1:4878930/1‑150 (MQ=255)
                                                                                   ccAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTgg                                                                <  2:2946497/150‑1 (MQ=255)
                                                                                    cAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGt                                                               <  2:658318/150‑1 (MQ=255)
                                                                                     aGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTa                                                              >  1:3533092/1‑150 (MQ=255)
                                                                                     aGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTa                                                              >  2:1051792/1‑150 (MQ=255)
                                                                                      gCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTAt                                                             <  2:1715642/150‑1 (MQ=255)
                                                                                        atatCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGATGGGTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGt                                                           >  2:1539511/1‑150 (MQ=255)
                                                                                          atCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTaa                                                         <  2:3033184/150‑1 (MQ=255)
                                                                                          atCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGAGGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTaa                                                         <  2:4782608/150‑1 (MQ=255)
                                                                                            ccTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAAcc                                                       <  1:1808420/150‑1 (MQ=255)
                                                                                              ttattaACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATCTAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCt                                                     >  2:4940448/1‑150 (MQ=255)
                                                                                                        aGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGAt                                           >  1:3294660/1‑150 (MQ=255)
                                                                                                        aGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGAt                                           >  2:3007150/1‑150 (MQ=255)
                                                                                                        aGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGAt                                           <  1:3569773/150‑1 (MQ=255)
                                                                                                         gtgtAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGTTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATg                                          <  2:1371633/150‑1 (MQ=255)
                                                                                                          tgtAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGt                                         >  1:366352/1‑150 (MQ=255)
                                                                                                           gtAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGtt                                        >  2:93271/1‑150 (MQ=255)
                                                                                                           gtAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGtt                                        >  2:350317/1‑150 (MQ=255)
                                                                                                           gtAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGtt                                        >  1:1221838/1‑150 (MQ=255)
                                                                                                            tAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTg                                       >  2:4088255/1‑150 (MQ=255)
                                                                                                            tAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTg                                       <  2:808805/150‑1 (MQ=255)
                                                                                                             aGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGa                                      >  1:154340/1‑150 (MQ=255)
                                                                                                              gggAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGAt                                     >  1:4022251/1‑150 (MQ=255)
                                                                                                               ggAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGAtt                                    >  2:1791992/1‑150 (MQ=255)
                                                                                                               ggAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATAATTGTCATTTGACCAGGCTCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGAtg                                    <  2:4771114/150‑2 (MQ=255)
                                                                                                                  aaGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATATCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATCTAACCCTAACTTAAGATGTTGATTCgg                                 >  1:3070687/1‑150 (MQ=255)
                                                                                                                   aGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGgc                                <  1:4004205/150‑1 (MQ=255)
                                                                                                                      atatCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGcgcg                             >  1:1060225/1‑150 (MQ=255)
                                                                                                                       tatCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCgcga                            >  1:3803409/1‑150 (MQ=255)
                                                                                                                         tCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCCTTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCGAACTTAAGATGTGGATTCGGCgcgagc                          <  2:3785356/150‑1 (MQ=255)
                                                                                                                         tCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCgcgagc                          >  1:1828972/1‑150 (MQ=255)
                                                                                                                            gATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGcgagcgag                       >  1:4279221/1‑150 (MQ=255)
                                                                                                                            gATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGcgagcgag                       <  2:3615211/150‑1 (MQ=255)
                                                                                                                                 tgGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTaa                  >  2:584646/1‑150 (MQ=255)
                                                                                                                                         ggctggctGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCg          <  1:258593/150‑1 (MQ=255)
                                                                                                                                          gctggctgGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGt         >  2:4075146/1‑150 (MQ=255)
                                                                                                                                            tggctggATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTaa       <  1:5110376/150‑1 (MQ=255)
                                                                                                                                            tggctggATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTaa       <  1:1348299/150‑1 (MQ=255)
                                                                                                                                                tggAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAg   >  1:4724798/1‑150 (MQ=255)
                                                                                                                                                 ggATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGa  >  2:4147584/1‑150 (MQ=255)
                                                                                                                                                 ggAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGa  <  1:2808818/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGA  >  CP009273/473990‑474284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACA  >  CP009273/473990‑474286
                                                                                                                                                    |                                                                                                                                                    
CAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCTCCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATA                                                                                                                                                     >  SRR19973083.872639/1‑150 (MQ=60)
 AGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATAT                                                                                                                                                    <  SRR19973083.3465072/150‑1 (MQ=60)
 AGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATAT                                                                                                                                                    <  SRR19973083.521722/150‑1 (MQ=60)
    CAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAG                                                                                                                                                 <  SRR19973083.2163833/150‑1 (MQ=60)
    CAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAG                                                                                                                                                 >  SRR19973083.400246/1‑150 (MQ=60)
        TGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGACAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGG                                                                                                                                             >  SRR19973083.2960857/1‑150 (MQ=60)
        TGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGG                                                                                                                                             <  SRR19973083.5076351/150‑1 (MQ=60)
        TGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGG                                                                                                                                             >  SRR19973083.1521768/1‑150 (MQ=60)
        TGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGG                                                                                                                                             <  SRR19973083.5076347/150‑1 (MQ=60)
            TAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGA                                                                                                                                         >  SRR19973083.870480/1‑150 (MQ=60)
                 CGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGG                                                                                                                                    >  SRR19973083.2610743/1‑150 (MQ=60)
                   ACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTA                                                                                                                                  <  SRR19973083.1212314/150‑1 (MQ=60)
                     AGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATT                                                                                                                                <  SRR19973083.3439208/150‑1 (MQ=60)
                         AGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCAT                                                                                                                            <  SRR19973083.4943471/150‑1 (MQ=60)
                           CAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTTACACAGTGTAGGGATAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGA                                                                                                                          <  SRR19973083.5186797/150‑1 (MQ=60)
                           CAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGA                                                                                                                          >  SRR19973083.3505766/1‑150 (MQ=60)
                            AATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAA                                                                                                                         >  SRR19973083.1525860/1‑150 (MQ=60)
                            AATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAA                                                                                                                         >  SRR19973083.990213/1‑150 (MQ=60)
                             ATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAG                                                                                                                        <  SRR19973083.3627741/150‑1 (MQ=60)
                                      GAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTG                                                                                                               <  SRR19973083.4468518/150‑1 (MQ=60)
                                           AGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAAC                                                                                                          <  SRR19973083.4022948/150‑1 (MQ=60)
                                           AGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAAC                                                                                                          <  SRR19973083.1391611/150‑1 (MQ=60)
                                                 CCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCAT                                                                                                    <  SRR19973083.1610948/150‑1 (MQ=60)
                                                  CCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATT                                                                                                   <  SRR19973083.2213309/150‑1 (MQ=60)
                                                  CCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTGATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATT                                                                                                   <  SRR19973083.4969133/150‑1 (MQ=60)
                                                    GAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGT                                                                                                 <  SRR19973083.4280118/150‑1 (MQ=60)
                                                    GAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGT                                                                                                 <  SRR19973083.4279871/150‑1 (MQ=60)
                                                    GAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGT                                                                                                 <  SRR19973083.4280012/150‑1 (MQ=60)
                                                        TAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATT                                                                                             <  SRR19973083.2930424/150‑1 (MQ=60)
                                                          AGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTG                                                                                           <  SRR19973083.3321129/150‑1 (MQ=60)
                                                            GCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGAC                                                                                         <  SRR19973083.3909703/150‑1 (MQ=60)
                                                            GCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGAC                                                                                         <  SRR19973083.3102746/150‑1 (MQ=60)
                                                            GCCGACCAGATGTCGCGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGAC                                                                                         <  SRR19973083.3102801/150‑1 (MQ=60)
                                                              CGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTNATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCA                                                                                       <  SRR19973083.4809110/150‑1 (MQ=60)
                                                              CGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCA                                                                                       <  SRR19973083.1077037/150‑1 (MQ=60)
                                                              CGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCA                                                                                       >  SRR19973083.4577144/1‑150 (MQ=60)
                                                               GACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGGGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAG                                                                                      <  SRR19973083.2272414/150‑1 (MQ=60)
                                                                ACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGACAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGG                                                                                     >  SRR19973083.2681528/1‑150 (MQ=60)
                                                                ACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGG                                                                                     >  SRR19973083.5126234/1‑150 (MQ=60)
                                                                 CCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATACGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGC                                                                                    >  SRR19973083.2049483/1‑150 (MQ=60)
                                                                 CCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGC                                                                                    <  SRR19973083.867517/150‑1 (MQ=60)
                                                                 CCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGC                                                                                    <  SRR19973083.867506/150‑1 (MQ=60)
                                                                 CCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGC                                                                                    >  SRR19973083.3556553/1‑150 (MQ=60)
                                                                    GATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCA                                                                                 >  SRR19973083.2322976/1‑150 (MQ=60)
                                                                     ATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAG                                                                                <  SRR19973083.4870661/150‑1 (MQ=60)
                                                                       GTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGACAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCAGTGTCATTTGACCAGGCGCAGAG                                                                              >  SRR19973083.3945417/1‑150 (MQ=60)
                                                                       GTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAG                                                                              >  SRR19973083.4935026/1‑150 (MQ=60)
                                                                        TCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGC                                                                             <  SRR19973083.5116767/150‑1 (MQ=60)
                                                                          GTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTT                                                                           >  SRR19973083.332662/1‑150 (MQ=60)
                                                                            GTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTA                                                                         <  SRR19973083.953260/150‑1 (MQ=60)
                                                                            GTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTA                                                                         <  SRR19973083.953264/150‑1 (MQ=60)
                                                                            GTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTA                                                                         >  SRR19973083.1113366/1‑150 (MQ=60)
                                                                                TCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCC                                                                     >  SRR19973083.5065308/1‑150 (MQ=60)
                                                                                  ACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTG                                                                   >  SRR19973083.4899879/1‑150 (MQ=60)
                                                                                   CCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGG                                                                  <  SRR19973083.3055544/150‑1 (MQ=60)
                                                                                     AGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTA                                                                >  SRR19973083.3549309/1‑150 (MQ=60)
                                                                                       CATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATG                                                              <  SRR19973083.3917507/150‑1 (MQ=60)
                                                                                            CCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACC                                                         <  SRR19973083.1817501/150‑1 (MQ=60)
                                                                                                  TAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACT                                                   <  SRR19973083.2091366/150‑1 (MQ=60)
                                                                                                       CAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGA                                              <  SRR19973083.1684403/150‑1 (MQ=60)
                                                                                                        AGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTGACCCTAACTTAAGAT                                             >  SRR19973083.3310103/1‑150 (MQ=60)
                                                                                                        AGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGAT                                             <  SRR19973083.1569947/150‑1 (MQ=60)
                                                                                                        AGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGAT                                             <  SRR19973083.3586144/150‑1 (MQ=60)
                                                                                                        AGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGAT                                             >  SRR19973083.3310158/1‑150 (MQ=60)
                                                                                                        AGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGAT                                             >  SRR19973083.3310159/1‑150 (MQ=60)
                                                                                                          TGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGT                                           >  SRR19973083.368079/1‑150 (MQ=60)
                                                                                                           GTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTT                                          >  SRR19973083.1227847/1‑150 (MQ=60)
                                                                                                            TAGGGAAAGTATATCTGATTGTGGAAATAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAAAGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTG                                         <  SRR19973083.1944417/150‑1 (MQ=60)
                                                                                                             AGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGA                                        >  SRR19973083.1820612/1‑150 (MQ=60)
                                                                                                             AGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGA                                        >  SRR19973083.155249/1‑150 (MQ=60)
                                                                                                              GGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGAT                                       >  SRR19973083.4040129/1‑150 (MQ=60)
                                                                                                               GGAAAGTATCTCTGATTGTGGAAAAAGGCTTGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGTTCATTGTCATTTGACCAGGCGCAGAGCTTTTCGCCTGGTTTGTAAGCCTAACTTAAGATGTTGATT                                      <  SRR19973083.918554/150‑1 (MQ=60)
                                                                                                                GAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTC                                     <  SRR19973083.1436665/150‑1 (MQ=60)
                                                                                                                GAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTC                                     <  SRR19973083.4692357/150‑1 (MQ=60)
                                                                                                                GAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTC                                     <  SRR19973083.959445/150‑1 (MQ=60)
                                                                                                                GAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTC                                     <  SRR19973083.3077435/150‑1 (MQ=60)
                                                                                                                 AAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCG                                    <  SRR19973083.4180142/150‑1 (MQ=60)
                                                                                                                  AAGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATATCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATCTAACCCTAACTTAAGATGTTGATTCGG                                   >  SRR19973083.3085416/1‑150 (MQ=60)
                                                                                                                   AGTATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGC                                  <  SRR19973083.4022016/150‑1 (MQ=60)
                                                                                                                      ATATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCG                               >  SRR19973083.1065565/1‑150 (MQ=60)
                                                                                                                       TATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGA                              >  SRR19973083.3584900/1‑150 (MQ=60)
                                                                                                                       TATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGA                              >  SRR19973083.3820525/1‑150 (MQ=60)
                                                                                                                       TATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGA                              <  SRR19973083.1791438/150‑1 (MQ=60)
                                                                                                                        ATCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAG                             >  SRR19973083.2241125/1‑150 (MQ=60)
                                                                                                                         TCTGATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGC                            >  SRR19973083.1838175/1‑150 (MQ=60)
                                                                                                                            GATTGTGGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAG                         >  SRR19973083.4297874/1‑150 (MQ=60)
                                                                                                                                 TGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAA                    <  SRR19973083.4747693/150‑1 (MQ=60)
                                                                                                                                  GGAAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAA                   >  SRR19973083.2600874/1‑150 (MQ=60)
                                                                                                                                    AAAAAGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAA                 <  SRR19973083.3766112/150‑1 (MQ=60)
                                                                                                                                        AGGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATC             <  SRR19973083.5189505/150‑1 (MQ=60)
                                                                                                                                         GGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCG            >  SRR19973083.2777327/1‑150 (MQ=60)
                                                                                                                                         GGCTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCG            <  SRR19973083.259966/150‑1 (MQ=60)
                                                                                                                                           CTGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTA          >  SRR19973083.52846/1‑150 (MQ=60)
                                                                                                                                            TGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAA         <  SRR19973083.1354927/150‑1 (MQ=60)
                                                                                                                                            TGGCTGGATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAA         <  SRR19973083.5132160/150‑1 (MQ=60)
                                                                                                                                                TGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAG     >  SRR19973083.4744995/1‑150 (MQ=60)
                                                                                                                                                 GGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGA    <  SRR19973083.1302191/150‑1 (MQ=60)
                                                                                                                                                 GGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGA    <  SRR19973083.2822741/150‑1 (MQ=60)
                                                                                                                                                   ATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACA  <  SRR19973083.4345484/150‑1 (MQ=60)
                                                                                                                                                   ATATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACA  >  SRR19973083.4365756/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                    
CAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACA  >  CP009273/473990‑474286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑