Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F181 I0 R1 1262 323.1 10297870 97.5% 10040423 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,586,791 G→T 26.3% G113G (GGC→GGA)  ypfH ← palmitoyl‑CoA esterase activity, uncertain physiological substrate

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,586,7910GT26.3% 657.9 / 205.5 289G113G (GGC→GGA) ypfHpalmitoyl‑CoA esterase activity, uncertain physiological substrate
Reads supporting (aligned to +/- strand):  ref base G (103/110);  new base T (42/34);  total (145/144)
Fisher's exact test for biased strand distribution p-value = 3.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 302 total aligned reads displayed.

TCGATAACCGGATCTTCACCGCCATGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTG  >  CP009273/2586642‑2586940
                                                                                                                                                     |                                                                                                                                                     
tCGATAACCGGATCTTCACCGCCATGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATcgcg                                                                                                                                                       <  2:2978308/150‑1 (MQ=255)
        cGGATCTTCACCGCCATGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTgaga                                                                                                                                               >  1:3104286/1‑150 (MQ=255)
         ggATCTTCACCGCCATGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTgagag                                                                                                                                              >  2:4458735/1‑150 (MQ=255)
          gATCTTCACCGCCATGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTgagaga                                                                                                                                             >  1:3299490/1‑150 (MQ=255)
          gATCTTCACCGCCATGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTgagaga                                                                                                                                             <  2:2130022/150‑1 (MQ=255)
          gATCTTCACCGCCATGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTgagaga                                                                                                                                             >  1:4220889/1‑150 (MQ=255)
           aTCTTCACCGCCATGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGaa                                                                                                                                            >  2:3224320/1‑150 (MQ=255)
             cTTCACCGCCATGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAAc                                                                                                                                          <  2:3262761/150‑1 (MQ=255)
             cTTCACCGCCATGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAAc                                                                                                                                          <  1:3140252/150‑1 (MQ=255)
                cACCGCCATGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGa                                                                                                                                       >  1:373820/1‑150 (MQ=255)
                 aCCGCCATGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGAt                                                                                                                                      >  2:4884399/1‑150 (MQ=255)
                  ccGCCATGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATg                                                                                                                                     >  2:3357207/1‑150 (MQ=255)
                    gCCATGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAg                                                                                                                                   >  2:3166782/1‑150 (MQ=255)
                     ccATGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAgc                                                                                                                                  >  2:766419/1‑150 (MQ=255)
                       ttGATTCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAgcgc                                                                                                                                <  2:3405128/149‑1 (MQ=255)
                        tGAATCAGGTTAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGcc                                                                                                                               <  1:2733536/150‑1 (MQ=255)
                          aaTCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGt                                                                                                                             >  1:3008248/1‑150 (MQ=255)
                           aTCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTg                                                                                                                            >  2:1236634/1‑150 (MQ=255)
                                gTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGtt                                                                                                                       >  2:3910958/1‑150 (MQ=255)
                                   aaTTGTCGTGGCTGTAGACGCAGTTTCCGGCATGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCTCGCCTTGAGAGAAACCGATGAGCGCCGTGTCGTTAga                                                                                                                    >  1:4937492/1‑149 (MQ=255)
                                   aaTTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGc                                                                                                                    <  2:2718266/150‑1 (MQ=255)
                                    aTTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGcc                                                                                                                   <  2:4277561/150‑1 (MQ=255)
                                       gTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCt                                                                                                                >  1:4598768/1‑150 (MQ=255)
                                       gTCGTGGCTGTAGACGCAGTGTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCt                                                                                                                <  2:3308054/150‑1 (MQ=255)
                                         cGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTAc                                                                                                              >  1:870400/1‑150 (MQ=255)
                                           tGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTc                                                                                                            <  1:4140232/150‑1 (MQ=255)
                                            ggCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTcc                                                                                                           >  2:2343074/1‑150 (MQ=255)
                                            ggCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTcc                                                                                                           <  2:1569391/150‑1 (MQ=255)
                                            ggCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTcc                                                                                                           <  2:4130644/150‑1 (MQ=255)
                                               tGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGct                                                                                                        >  1:57328/1‑150 (MQ=255)
                                               tGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGct                                                                                                        <  1:629634/150‑1 (MQ=255)
                                               tGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGct                                                                                                        <  1:1335233/150‑1 (MQ=255)
                                                 tAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGctct                                                                                                      <  2:1787934/150‑1 (MQ=255)
                                                        aGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTg                                                                                               <  2:1164589/150‑1 (MQ=255)
                                                        aGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACAATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTg                                                                                               >  1:1497365/1‑150 (MQ=255)
                                                         gTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGc                                                                                              <  1:521615/150‑1 (MQ=255)
                                                            tCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAg                                                                                           <  1:2128697/150‑1 (MQ=255)
                                                             ccGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGt                                                                                          >  2:140023/1‑150 (MQ=255)
                                                              cGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTa                                                                                         <  1:719794/150‑1 (MQ=255)
                                                                gCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAgc                                                                                       <  1:79168/150‑1 (MQ=255)
                                                                  aGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAgcgc                                                                                     >  2:3530807/1‑150 (MQ=255)
                                                                     cTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCAcc                                                                                  <  2:2855661/150‑1 (MQ=255)
                                                                      tCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCg                                                                                 <  2:2740001/150‑1 (MQ=255)
                                                                      tCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCg                                                                                 <  1:365737/150‑1 (MQ=255)
                                                                         cATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGttt                                                                              >  1:5051705/1‑150 (MQ=255)
                                                                           tAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCa                                                                            <  2:1157800/150‑1 (MQ=255)
                                                                           tAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCa                                                                            <  1:3651426/150‑1 (MQ=255)
                                                                              cGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATa                                                                         >  2:2989051/1‑150 (MQ=255)
                                                                              cGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATa                                                                         >  1:4293868/1‑150 (MQ=255)
                                                                                  cGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACg                                                                     <  2:1859723/150‑1 (MQ=255)
                                                                                     tAAAAGCAATGACGCGTGATGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCg                                                                  <  2:3822706/150‑1 (MQ=255)
                                                                                      aaaaGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCgg                                                                 <  2:4616727/150‑1 (MQ=255)
                                                                                      aaaaGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCgg                                                                 >  1:3437973/1‑150 (MQ=255)
                                                                                       aaaGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGc                                                                >  1:4468552/1‑150 (MQ=255)
                                                                                           cAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGa                                                            <  2:5056465/150‑1 (MQ=255)
                                                                                           cAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGa                                                            <  2:4193794/150‑1 (MQ=255)
                                                                                            aaTGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGAt                                                           >  2:2761343/1‑150 (MQ=255)
                                                                                             aTGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATc                                                          <  2:1715130/150‑1 (MQ=255)
                                                                                               gACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGc                                                        >  1:2220334/1‑150 (MQ=255)
                                                                                                   cgTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATcc                                                    >  1:3505757/1‑150 (MQ=255)
                                                                                                   cgTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATcc                                                    >  2:3989243/1‑150 (MQ=255)
                                                                                                   cgTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGGTTCAATCACCGTCGGCATGATCGCATcc                                                    >  2:3989280/1‑150 (MQ=255)
                                                                                                    gTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCca                                                   >  2:919340/1‑150 (MQ=255)
                                                                                                    gTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCca                                                   >  2:3705623/1‑150 (MQ=255)
                                                                                                      gAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCcaca                                                 >  1:4000880/1‑150 (MQ=255)
                                                                                                      gAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCcaca                                                 >  2:4715296/1‑150 (MQ=255)
                                                                                                        aGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACAcg                                               <  1:1721743/150‑1 (MQ=255)
                                                                                                        aGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACAcg                                               <  1:4884399/150‑1 (MQ=255)
                                                                                                          cAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACAcgcg                                             <  2:40491/150‑1 (MQ=255)
                                                                                                          cAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACAcgcg                                             >  2:723379/1‑150 (MQ=255)
                                                                                                           aaGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACgcgc                                            <  1:4792434/150‑1 (MQ=255)
                                                                                                           aaGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACgcgc                                            >  2:3279494/1‑150 (MQ=255)
                                                                                                                ccGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGc                                       <  2:3944061/150‑1 (MQ=255)
                                                                                                                  ggTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGa                                     >  2:1094539/1‑150 (MQ=255)
                                                                                                                  ggTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGa                                     <  1:1059633/150‑1 (MQ=255)
                                                                                                                  ggTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGa                                     <  2:3815155/150‑1 (MQ=255)
                                                                                                                  ggTTCGGCTTTAATGATCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCACCTAATCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGa                                     >  1:2920544/1‑150 (MQ=255)
                                                                                                                    ttCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGAtt                                   >  1:4661872/1‑150 (MQ=255)
                                                                                                                     tCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTa                                  >  1:4561696/1‑150 (MQ=255)
                                                                                                                       ggCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATc                                >  1:2924567/1‑150 (MQ=255)
                                                                                                                       ggCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATc                                >  2:2920527/1‑150 (MQ=255)
                                                                                                                       ggCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCCTCCACACGCGCCTGGCGATtctc                                >  2:2674861/1‑150 (MQ=255)
                                                                                                                       ggCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATc                                <  1:2006271/150‑1 (MQ=255)
                                                                                                                        gCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCt                               <  2:57328/150‑1 (MQ=255)
                                                                                                                         cTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCtt                              <  2:1756294/150‑1 (MQ=255)
                                                                                                                          tttAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTc                             >  2:255990/1‑150 (MQ=255)
                                                                                                                             aaTGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGt                          >  2:4675280/1‑150 (MQ=255)
                                                                                                                              aTGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTa                         <  2:1480989/150‑1 (MQ=255)
                                                                                                                                gCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCCCACGCGCCTGGCGAGTATCTTCCGTaag                       >  2:658375/1‑149 (MQ=255)
                                                                                                                                 ctctCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATa                      >  2:3060065/1‑150 (MQ=255)
                                                                                                                                    tcCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATAccc                   <  1:3241890/150‑1 (MQ=255)
                                                                                                                                          aCCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCAcc             <  1:3394715/150‑1 (MQ=255)
                                                                                                                                          aCCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCAcc             >  1:3593860/1‑150 (MQ=255)
                                                                                                                                           ccATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCg            <  1:1632730/150‑1 (MQ=255)
                                                                                                                                            cATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGa           >  1:4680572/1‑150 (MQ=255)
                                                                                                                                             aTAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGac          >  1:3890684/1‑149 (MQ=255)
                                                                                                                                                aTCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAAc       >  1:59128/1‑150 (MQ=255)
                                                                                                                                                aTCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAAc       <  1:2678540/150‑1 (MQ=255)
                                                                                                                                                    cgcCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACt   <  2:3446153/150‑1 (MQ=255)
                                                                                                                                                     gcCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTg  <  2:827994/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCGATAACCGGATCTTCACCGCCATGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTG  >  CP009273/2586642‑2586940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A