Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F159 I0 R1 879 305.9 9588012 96.6% 9262019 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,586,791 G→T 31.6% G113G (GGC→GGA)  ypfH ← palmitoyl‑CoA esterase activity, uncertain physiological substrate

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,586,7910GT31.6% 618.6 / 312.9 342G113G (GGC→GGA) ypfHpalmitoyl‑CoA esterase activity, uncertain physiological substrate
Reads supporting (aligned to +/- strand):  ref base G (122/112);  new base T (47/61);  total (169/173)
Fisher's exact test for biased strand distribution p-value = 1.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.63e-01

Only 100 of 351 total aligned reads displayed.

TCGATAACCGGATCTTCACCGCCATGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTG  >  CP009273/2586642‑2586940
                                                                                                                                                     |                                                                                                                                                     
tCGATAACCGGATCTTCACCGCCATGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATcgcg                                                                                                                                                       <  1:3896317/150‑1 (MQ=255)
   aTAACCGGATCTTCACCGCCATGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCt                                                                                                                                                    <  2:651959/150‑1 (MQ=255)
    gcaCCGGATCTTCACCGCCATGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCta                                                                                                                                                   >  1:994692/3‑149 (MQ=255)
     aaCCGGATCTTCACCGCCATGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTg                                                                                                                                                  >  2:162481/1‑150 (MQ=255)
      aCCGGATCTTCACCGCCATGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTga                                                                                                                                                 >  1:55962/1‑150 (MQ=255)
         ggATCTTCACCGCCATGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTgagag                                                                                                                                              <  2:3803468/150‑1 (MQ=255)
          gATCTTCACCGCCATGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTgagaga                                                                                                                                             <  1:2814552/150‑1 (MQ=255)
           aTCTTCACCGCCATGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGaa                                                                                                                                            >  1:775365/1‑150 (MQ=255)
           aTCTTCACCGCCATGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGaa                                                                                                                                            >  2:2305307/1‑150 (MQ=255)
             cTTCACCGCCATGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAAc                                                                                                                                          >  1:1308451/1‑150 (MQ=255)
              ttCACCGCCATGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAAcc                                                                                                                                         <  2:1405286/150‑1 (MQ=255)
               tCACCGCCATGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCg                                                                                                                                        <  1:3174288/150‑1 (MQ=255)
                cACCGCCATGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGa                                                                                                                                       <  1:4581344/150‑1 (MQ=255)
                  ccGCCATGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATg                                                                                                                                     <  1:286729/150‑1 (MQ=255)
                    gCCATGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAg                                                                                                                                   <  1:4117463/150‑1 (MQ=255)
                     ccATGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAgc                                                                                                                                  <  1:1120012/150‑1 (MQ=255)
                      cATGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAgcg                                                                                                                                 <  2:2623457/150‑1 (MQ=255)
                         gAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCg                                                                                                                              >  1:2601962/1‑150 (MQ=255)
                            tCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTgg                                                                                                                           >  2:4174632/1‑150 (MQ=255)
                             cAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGc                                                                                                                          >  1:2375177/1‑150 (MQ=255)
                             cAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGc                                                                                                                          <  2:4745273/150‑1 (MQ=255)
                                  gAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGATTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAg                                                                                                                     <  1:865083/150‑1 (MQ=255)
                                        tCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTa                                                                                                               <  1:708702/150‑1 (MQ=255)
                                        tCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTa                                                                                                               <  1:3422995/150‑1 (MQ=255)
                                        tCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTa                                                                                                               <  2:3677752/150‑1 (MQ=255)
                                         cGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTAc                                                                                                              >  2:2239671/1‑150 (MQ=255)
                                         cGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTAc                                                                                                              <  1:4107886/150‑1 (MQ=255)
                                            ggCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTcc                                                                                                           >  1:3231795/1‑150 (MQ=255)
                                             gCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCg                                                                                                          >  1:1494122/1‑150 (MQ=255)
                                                gTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGctc                                                                                                       >  2:4413166/1‑150 (MQ=255)
                                                 tAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGctct                                                                                                      <  2:2090351/150‑1 (MQ=255)
                                                 tAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGctct                                                                                                      <  2:1164017/150‑1 (MQ=255)
                                                     cGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGttt                                                                                                  <  2:1450402/150‑1 (MQ=255)
                                                           ttCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCa                                                                                            >  1:3666144/1‑150 (MQ=255)
                                                           ttCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCa                                                                                            <  2:3744757/150‑1 (MQ=255)
                                                           ttCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCa                                                                                            <  2:1888321/150‑1 (MQ=255)
                                                              cGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAgaa                                                                                         >  2:4020727/1‑148 (MQ=255)
                                                              cGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTa                                                                                         <  1:2913775/150‑1 (MQ=255)
                                                              cGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTa                                                                                         <  2:1315626/150‑1 (MQ=255)
                                                               ggCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACTATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAg                                                                                        <  2:2543122/150‑1 (MQ=255)
                                                                gCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAgc                                                                                       <  1:4608273/150‑1 (MQ=255)
                                                                 cAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAgcg                                                                                      >  1:404726/1‑150 (MQ=255)
                                                                 cAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAgcg                                                                                      <  2:2227777/150‑1 (MQ=255)
                                                                     cTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCAcc                                                                                  >  2:834849/1‑150 (MQ=255)
                                                                      tCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCg                                                                                 >  2:2795410/1‑150 (MQ=255)
                                                                       cGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGt                                                                                <  1:2493214/150‑1 (MQ=255)
                                                                       cGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCATACTCCGCTCTGTTTCTGCAAGTAGCGCACCGt                                                                                >  1:515784/1‑150 (MQ=255)
                                                                        gCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGtt                                                                               <  2:1535351/150‑1 (MQ=255)
                                                                           tAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCa                                                                            >  2:1761435/1‑150 (MQ=255)
                                                                             gCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAAt                                                                          <  1:887690/150‑1 (MQ=255)
                                                                             gCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAAt                                                                          <  2:2473100/150‑1 (MQ=255)
                                                                               gTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATaa                                                                        >  2:2993250/1‑150 (MQ=255)
                                                                               gTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATaa                                                                        <  1:2333713/150‑1 (MQ=255)
                                                                               gTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATaa                                                                        >  1:3536853/1‑150 (MQ=255)
                                                                                 ccGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAAc                                                                      >  2:3940116/1‑150 (MQ=255)
                                                                                 ccGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAAc                                                                      >  1:1814385/1‑150 (MQ=255)
                                                                                    ttAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTc                                                                   <  2:3200452/150‑1 (MQ=255)
                                                                                      aaaaGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCgg                                                                 >  2:4291320/1‑150 (MQ=255)
                                                                                         aGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCAt                                                              <  2:743057/150‑1 (MQ=255)
                                                                                         aGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCAt                                                              >  2:104818/1‑150 (MQ=255)
                                                                                           cAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGa                                                            >  1:639919/1‑150 (MQ=255)
                                                                                              tGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCg                                                         <  1:1964288/150‑1 (MQ=255)
                                                                                              tGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCg                                                         >  1:4196520/1‑150 (MQ=255)
                                                                                              tGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCg                                                         >  1:4727371/1‑150 (MQ=255)
                                                                                                   cgTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATcc                                                    >  2:554490/1‑150 (MQ=255)
                                                                                                    gTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCca                                                   >  2:348886/1‑150 (MQ=255)
                                                                                                    gTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCca                                                   <  1:2522194/150‑1 (MQ=255)
                                                                                                     tGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCcac                                                  >  1:4243955/1‑150 (MQ=255)
                                                                                                     tGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCcac                                                  >  1:1005793/1‑150 (MQ=255)
                                                                                                       aaGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCacac                                                <  2:3019692/150‑1 (MQ=255)
                                                                                                       aaGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCacac                                                <  1:105600/150‑1 (MQ=255)
                                                                                                       aaGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCacac                                                >  1:1294106/1‑150 (MQ=255)
                                                                                                       aaGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCacac                                                >  1:161699/1‑150 (MQ=255)
                                                                                                           aaGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACgcgc                                            >  1:4480974/1‑150 (MQ=255)
                                                                                                            aGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGcc                                           <  2:1979507/150‑1 (MQ=255)
                                                                                                             ggCCCGGTTCGGCTTTAATGCTCTCCAGCACCATACTCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCt                                          <  1:1575932/150‑1 (MQ=255)
                                                                                                             ggCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCt                                          >  2:943369/1‑150 (MQ=255)
                                                                                                             ggCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCt                                          <  1:4374106/150‑1 (MQ=255)
                                                                                                              gCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTg                                         <  2:1721100/150‑1 (MQ=255)
                                                                                                              gCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTg                                         <  1:4284735/150‑1 (MQ=255)
                                                                                                                 cGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCg                                      <  2:4525114/150‑1 (MQ=255)
                                                                                                                  ggTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGa                                     >  1:1917278/1‑150 (MQ=255)
                                                                                                                           ttAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTcc                            <  1:2155546/150‑1 (MQ=255)
                                                                                                                            tAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCg                           >  1:3059072/1‑150 (MQ=255)
                                                                                                                              gtGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTa                         >  1:2630888/2‑150 (MQ=255)
                                                                                                                              aTGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTa                         >  1:1409679/1‑150 (MQ=255)
                                                                                                                               tGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTaa                        >  1:4357180/1‑150 (MQ=255)
                                                                                                                                gCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAAt                       <  1:1690258/150‑1 (MQ=255)
                                                                                                                                gCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAAt                       <  2:3391203/150‑1 (MQ=255)
                                                                                                                                      cAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTg                 >  1:909049/1‑150 (MQ=255)
                                                                                                                                       aGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGc                <  1:208306/150‑1 (MQ=255)
                                                                                                                                          aCCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCAcc             >  2:3941821/1‑150 (MQ=255)
                                                                                                                                             aTAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGaa          >  2:1514151/1‑150 (MQ=255)
                                                                                                                                             aTAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGaa          >  1:873746/1‑150 (MQ=255)
                                                                                                                                              tAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGaaa         >  1:2190415/1‑150 (MQ=255)
                                                                                                                                              tAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGaaa         >  1:946919/1‑150 (MQ=255)
                                                                                                                                                 tCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAAcc      >  2:4609551/1‑150 (MQ=255)
                                                                                                                                                  cgcgCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAATCGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGACAACCa     >  2:4343976/1‑150 (MQ=255)
                                                                                                                                                     gcCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTg  >  1:1145728/1‑150 (MQ=255)
                                                                                                                                                     gcCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTg  >  1:2683353/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCGATAACCGGATCTTCACCGCCATGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCACTG  >  CP009273/2586642‑2586940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A