Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F202 I0 R1 1397 275.7 9089454 95.2% 8653160 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,586,791 G→T 14.0% G113G (GGC→GGA)  ypfH ← palmitoyl‑CoA esterase activity, uncertain physiological substrate

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,586,7910GT14.0% 882.3 / 82.1 270G113G (GGC→GGA) ypfHpalmitoyl‑CoA esterase activity, uncertain physiological substrate
Reads supporting (aligned to +/- strand):  ref base G (123/109);  new base T (13/25);  total (136/134)
Fisher's exact test for biased strand distribution p-value = 3.62e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 274 total aligned reads displayed.

TCGATAACCGGATCTTCACCGCCATGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCA  >  CP009273/2586642‑2586937
                                                                                                                                                     |                                                                                                                                                  
tCGATAACCGGATCTTCACCGCCATGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAACACCATAATcgcg                                                                                                                                                    <  1:1230739/150‑1 (MQ=255)
     aaCCGGATCTTCACCGCCATGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTg                                                                                                                                               >  1:2937896/1‑150 (MQ=255)
     aaCCGGATCTTCACCGCCATGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTg                                                                                                                                               >  2:178436/1‑150 (MQ=255)
       ccGGATCTTCACCGCCATGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTgag                                                                                                                                             <  2:634218/150‑1 (MQ=255)
        cGGATCTTCACCGCCATGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTgaga                                                                                                                                            <  1:3582668/150‑1 (MQ=255)
        cGGATCTTCACCGCCATGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTgaga                                                                                                                                            >  1:2559801/1‑150 (MQ=255)
         ggATCTTCACCGCCATGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTgagag                                                                                                                                           <  2:283379/150‑1 (MQ=255)
              ttCACCGCCATGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAAcc                                                                                                                                      <  2:2736272/150‑1 (MQ=255)
              ttCACCGCCATGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAAcc                                                                                                                                      >  2:1170191/1‑150 (MQ=255)
              ttCACCGCCATGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAAcc                                                                                                                                      <  2:2735870/150‑1 (MQ=255)
                cACCGCCATGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGa                                                                                                                                    >  1:1251666/1‑150 (MQ=255)
                cACCGCCATGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGa                                                                                                                                    >  2:2658505/1‑150 (MQ=255)
                  ccGCCATGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATg                                                                                                                                  <  1:3049569/150‑1 (MQ=255)
                  ccGCCATGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGAGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATg                                                                                                                                  >  1:4436736/1‑150 (MQ=255)
                   cGCCATGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGa                                                                                                                                 >  1:1979183/1‑150 (MQ=255)
                    gCCATGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAg                                                                                                                                >  1:2346378/1‑150 (MQ=255)
                        tGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGcc                                                                                                                            >  2:2481428/1‑150 (MQ=255)
                          aaTCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGt                                                                                                                          <  2:1650884/150‑1 (MQ=255)
                          aaTCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGt                                                                                                                          <  1:1602661/150‑1 (MQ=255)
                           aTCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTg                                                                                                                         >  1:4276433/1‑150 (MQ=255)
                           aTCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTg                                                                                                                         >  1:3976111/1‑150 (MQ=255)
                             cAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGc                                                                                                                       >  1:2852361/1‑150 (MQ=255)
                              aGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCg                                                                                                                      <  1:3361889/150‑1 (MQ=255)
                                gTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGtt                                                                                                                    <  2:3603644/150‑1 (MQ=255)
                                gTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGtt                                                                                                                    <  2:840023/150‑1 (MQ=255)
                                    aTTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGcc                                                                                                                <  1:2029468/150‑1 (MQ=255)
                                          gTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACt                                                                                                          >  2:3293953/1‑150 (MQ=255)
                                            ggCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGGTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTcc                                                                                                        <  2:418540/150‑1 (MQ=255)
                                                  aGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTg                                                                                                  <  2:1446604/150‑1 (MQ=255)
                                                    aCGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGtt                                                                                                >  1:539693/1‑150 (MQ=255)
                                                     cGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGttt                                                                                               >  2:1815735/1‑150 (MQ=255)
                                                       cAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCt                                                                                             <  2:2957656/150‑1 (MQ=255)
                                                            tCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAg                                                                                        <  2:1115617/150‑1 (MQ=255)
                                                             ccGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGt                                                                                       <  2:3773714/150‑1 (MQ=255)
                                                                 cAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTACTGCCAGTAgcg                                                                                   >  2:1544741/1‑150 (MQ=255)
                                                                   ggCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCa                                                                                 <  1:4470941/150‑1 (MQ=255)
                                                                       cGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGt                                                                             >  1:3423524/1‑150 (MQ=255)
                                                                       cGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGt                                                                             >  2:376422/1‑150 (MQ=255)
                                                                       cGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGt                                                                             >  1:2455030/1‑150 (MQ=255)
                                                                         cATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTGTCTGCCAGTAGCGCACCGttt                                                                           >  2:3008332/1‑150 (MQ=255)
                                                                         cATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGttt                                                                           >  1:2808958/1‑150 (MQ=255)
                                                                         cATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGttt                                                                           <  1:3748830/150‑1 (MQ=255)
                                                                          aTAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTc                                                                          >  2:4493529/1‑150 (MQ=255)
                                                                           tAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTCGCGCACCGTTTCa                                                                         >  1:2124868/1‑150 (MQ=255)
                                                                                   gTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGt                                                                 <  2:2083298/150‑1 (MQ=255)
                                                                                   gTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGt                                                                 >  2:1722120/1‑150 (MQ=255)
                                                                                    ttAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTc                                                                <  2:3369356/150‑1 (MQ=255)
                                                                                     tAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCg                                                               >  1:2785296/1‑150 (MQ=255)
                                                                                        aaGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCa                                                            <  2:2002472/150‑1 (MQ=255)
                                                                                        aaGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCa                                                            >  1:1389465/1‑150 (MQ=255)
                                                                                        aaGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCa                                                            <  1:3605125/150‑1 (MQ=255)
                                                                                        aaGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCa                                                            >  1:4157911/1‑150 (MQ=255)
                                                                                         aGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCAt                                                           <  1:206713/150‑1 (MQ=255)
                                                                                         aGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCAt                                                           >  1:3556022/1‑150 (MQ=255)
                                                                                              tGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCg                                                      <  2:2229116/150‑1 (MQ=255)
                                                                                                aCGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCa                                                    <  2:1639738/150‑1 (MQ=255)
                                                                                                 cgcgTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCAt                                                   >  1:287015/1‑150 (MQ=255)
                                                                                                  gcgTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATc                                                  <  1:1330034/150‑1 (MQ=255)
                                                                                                    gTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCca                                                <  1:3811957/150‑1 (MQ=255)
                                                                                                    gTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCca                                                <  2:1900787/150‑1 (MQ=255)
                                                                                                      gAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCcaca                                              >  1:331554/1‑150 (MQ=255)
                                                                                                           aaGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACgcgc                                         >  1:1820351/1‑150 (MQ=255)
                                                                                                           aaGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACgcgc                                         >  2:2174922/1‑150 (MQ=255)
                                                                                                             ggCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCt                                       <  2:2402963/150‑1 (MQ=255)
                                                                                                                ccGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGCTCGCATCCACACGCGCCTGGc                                    <  2:4246812/150‑1 (MQ=255)
                                                                                                                ccGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGc                                    <  1:2481428/150‑1 (MQ=255)
                                                                                                                   gTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGAt                                 <  2:4013844/150‑1 (MQ=255)
                                                                                                                   gTTCGGCTTTAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGAt                                 <  2:156171/150‑1 (MQ=255)
                                                                                                                   gTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGAt                                 <  2:455389/150‑1 (MQ=255)
                                                                                                                     tCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTa                               <  2:4309338/150‑1 (MQ=255)
                                                                                                                      cGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTAt                              >  2:706359/1‑150 (MQ=255)
                                                                                                                      cGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCAACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTAt                              <  2:727891/150‑1 (MQ=255)
                                                                                                                       ggCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATc                             >  2:554214/1‑150 (MQ=255)
                                                                                                                        gCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCt                            <  1:3868560/150‑1 (MQ=255)
                                                                                                                         cTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCtt                           >  1:2702482/1‑150 (MQ=255)
                                                                                                                         cTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCtt                           <  1:2767235/150‑1 (MQ=255)
                                                                                                                          tttAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTc                          >  1:1052384/1‑150 (MQ=255)
                                                                                                                           ttAATGCTCTCCAGCCCCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTcc                         >  1:3685862/1‑150 (MQ=255)
                                                                                                                           ttAATGCTCTCCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTcc                         <  2:1497757/150‑1 (MQ=255)
                                                                                                                             aaTGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGt                       >  2:1130200/1‑150 (MQ=255)
                                                                                                                               tGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTaa                     <  2:3337953/150‑1 (MQ=255)
                                                                                                                               tGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTaa                     >  1:767072/1‑150 (MQ=255)
                                                                                                                                gCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAAt                    <  1:2574518/150‑1 (MQ=255)
                                                                                                                                gCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAAt                    >  2:2440848/1‑150 (MQ=255)
                                                                                                                                  tctcCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATAc                  >  1:1542253/1‑150 (MQ=255)
                                                                                                                                  tctcCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATAc                  <  1:2598092/150‑1 (MQ=255)
                                                                                                                                  tctcCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATAc                  <  1:302169/150‑1 (MQ=255)
                                                                                                                                    tcCAGCACCATAATCGCTCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATAccc                >  2:1982303/1‑150 (MQ=255)
                                                                                                                                     ccAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCt               <  1:3963474/150‑1 (MQ=255)
                                                                                                                                      cAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTg              >  2:345314/1‑150 (MQ=255)
                                                                                                                                        gCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCa            <  2:4131607/150‑1 (MQ=255)
                                                                                                                                         cACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCAc           >  2:966040/1‑150 (MQ=255)
                                                                                                                                          aCCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCAcc          <  2:3531315/150‑1 (MQ=255)
                                                                                                                                          aCCATAATCGCGCCTTGAGACAAAGCGATGAGCGCCGTCGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCAcc          <  2:3672968/150‑1 (MQ=255)
                                                                                                                                           ccATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCg         <  2:4361555/150‑1 (MQ=255)
                                                                                                                                           ccATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCg         >  2:3969705/1‑150 (MQ=255)
                                                                                                                                             aTAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGaa       <  2:2564136/150‑1 (MQ=255)
                                                                                                                                               aaTCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGaaaa     <  2:20658/150‑1 (MQ=255)
                                                                                                                                                aTCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAAc    <  2:356760/150‑1 (MQ=255)
                                                                                                                                                  cgcgCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCa  >  1:1657702/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
TCGATAACCGGATCTTCACCGCCATGAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCCTTGAGAGAAACCGATGAGCGCCGTGGCGTTAGCCCCTACTCCGCTCTGTTTCTGCCAGTAGCGCACCGTTTCAATAAACGTCGGCATGATCGCATCCACACGCGCCTGGCGATTATCTTCCGTAATACCCTGCACCGAAAACCA  >  CP009273/2586642‑2586937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A