Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F181 I0 R1 1262 323.1 10297870 97.5% 10040423 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,009,187 G→T 39.4% intergenic (+90/‑232) mocA → / → ygfK CTP:molybdopterin cytidylyltransferase/putative Fe‑S subunit oxidoreductase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,009,1870GT39.4% 320.9 / inf 284intergenic (+90/‑232)mocA/ygfKCTP:molybdopterin cytidylyltransferase/putative Fe‑S subunit oxidoreductase subunit
Reads supporting (aligned to +/- strand):  ref base G (88/84);  new base T (50/62);  total (138/146)
Fisher's exact test for biased strand distribution p-value = 3.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01

Only 100 of 306 total aligned reads displayed.

TTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAAGAGTGGTGC  >  CP009273/3009038‑3009336
                                                                                                                                                     |                                                                                                                                                     
ttAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTgagaaaagag                                                                                                                                                       >  1:967942/1‑150 (MQ=255)
  aGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTgagaaaagataa                                                                                                                                                     <  2:1845280/150‑1 (MQ=255)
  aGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTgagaaaagagaa                                                                                                                                                     >  2:394617/1‑150 (MQ=255)
  aGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTgagaaaagagaa                                                                                                                                                     <  1:1642332/150‑1 (MQ=255)
       ttGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGACAAGAGAAAAAtt                                                                                                                                                >  2:1310299/1‑150 (MQ=255)
       ttGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAAtt                                                                                                                                                <  2:4760408/150‑1 (MQ=255)
            aCTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATaa                                                                                                                                           <  2:5046832/150‑1 (MQ=255)
              tCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAAt                                                                                                                                         >  2:3895140/1‑150 (MQ=255)
                cGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATgg                                                                                                                                       <  2:1947537/150‑1 (MQ=255)
                cGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATgg                                                                                                                                       >  2:5114956/1‑150 (MQ=255)
                 ggACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATggg                                                                                                                                      >  2:651152/1‑150 (MQ=255)
                  gacgacTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGc                                                                                                                                     <  1:4210836/150‑1 (MQ=255)
                  gacgacTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGc                                                                                                                                     >  1:4793534/1‑150 (MQ=255)
                  gacgacTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGc                                                                                                                                     >  1:5083321/1‑150 (MQ=255)
                  gacgacTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGc                                                                                                                                     <  1:2517935/150‑1 (MQ=255)
                    cgacTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTg                                                                                                                                   >  2:3533430/1‑150 (MQ=255)
                      acTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGc                                                                                                                                 <  1:2296978/150‑1 (MQ=255)
                       cTTTATTACCGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCa                                                                                                                                <  1:5141371/150‑1 (MQ=255)
                         ttattaCAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCaat                                                                                                                              <  2:4093661/150‑1 (MQ=255)
                         ttattaCAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCaat                                                                                                                              <  1:404299/150‑1 (MQ=255)
                          tattaCAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCaata                                                                                                                             >  1:447520/1‑150 (MQ=255)
                            ttaCAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCaataat                                                                                                                           >  2:4218143/1‑150 (MQ=255)
                               cAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCaataataat                                                                                                                        >  1:3897466/1‑150 (MQ=255)
                               cAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCaataataat                                                                                                                        <  2:2222402/150‑1 (MQ=255)
                                 gCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAAttt                                                                                                                      >  2:1740931/1‑150 (MQ=255)
                                  cGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAAtttt                                                                                                                     >  2:228479/1‑150 (MQ=255)
                                   gAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTc                                                                                                                    >  2:4410483/1‑150 (MQ=255)
                                    aaGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCa                                                                                                                   <  1:3115444/150‑1 (MQ=255)
                                    aaGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCa                                                                                                                   <  2:696006/150‑1 (MQ=255)
                                    aaGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCa                                                                                                                   >  1:2245573/1‑150 (MQ=255)
                                         aaGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGt                                                                                                              <  2:4654361/150‑1 (MQ=255)
                                         aaGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGt                                                                                                              >  2:4893253/1‑150 (MQ=255)
                                         aaGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGt                                                                                                              >  2:2090230/1‑150 (MQ=255)
                                            gTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGAt                                                                                                           <  1:1391031/150‑1 (MQ=255)
                                              ataCTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGAttt                                                                                                         >  2:216908/1‑150 (MQ=255)
                                               taCTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGAtttt                                                                                                        <  2:3364672/150‑1 (MQ=255)
                                               taCTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGAtttt                                                                                                        <  1:781098/150‑1 (MQ=255)
                                                    aaaTTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATtc                                                                                                   >  1:814800/1‑150 (MQ=255)
                                                    aaaTTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATtc                                                                                                   <  2:4120832/150‑1 (MQ=255)
                                                     aaTTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATtct                                                                                                  <  2:1112226/150‑1 (MQ=255)
                                                      aTTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATtctc                                                                                                 >  1:4913415/1‑150 (MQ=255)
                                                       tttAGAAAAGGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCa                                                                                                <  1:3394331/150‑1 (MQ=255)
                                                       tttAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCa                                                                                                >  1:3832626/1‑150 (MQ=255)
                                                           gAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTa                                                                                            >  2:4823579/1‑150 (MQ=255)
                                                              aaCGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCa                                                                                         <  2:4560658/150‑1 (MQ=255)
                                                                  tAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCtg                                                                                     <  1:4284821/150‑1 (MQ=255)
                                                                   aGTTAAACTATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCtgt                                                                                    <  2:14454/150‑1 (MQ=255)
                                                                   aGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCtgt                                                                                    <  1:2673894/150‑1 (MQ=255)
                                                                     ttAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGTTTTTAATTCTCATTTAGCACCTGTGa                                                                                  >  1:1031975/1‑150 (MQ=255)
                                                                      tAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAg                                                                                 <  1:4075789/150‑1 (MQ=255)
                                                                      tAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAg                                                                                 <  2:4677121/150‑1 (MQ=255)
                                                                          cGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGttca                                                                             <  2:2385054/150‑1 (MQ=255)
                                                                           gATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGttcat                                                                            <  1:682486/150‑1 (MQ=255)
                                                                            aTTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGttcatt                                                                           >  2:3754715/1‑150 (MQ=255)
                                                                            aTTGCGTTCAAATATTTAAGCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGttcatt                                                                           <  2:3623813/150‑1 (MQ=255)
                                                                             ttGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGttcattc                                                                          >  1:27614/1‑150 (MQ=255)
                                                                               gCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTcac                                                                        <  2:1822222/150‑1 (MQ=255)
                                                                                    cAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACaaaaa                                                                   <  1:2008420/150‑1 (MQ=255)
                                                                                    cAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACaaaaa                                                                   >  1:1575947/1‑150 (MQ=255)
                                                                                    cAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAAGTCTCATTTAGCACCTGTGAGTTCATTCACaaaaa                                                                   >  2:4944382/1‑150 (MQ=255)
                                                                                            tAATCCTTCCGTCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAATATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTaa                                                           <  2:5059580/150‑1 (MQ=255)
                                                                                            tAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTaa                                                           <  2:2464111/150‑1 (MQ=255)
                                                                                               tCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGtt                                                        >  2:267199/1‑150 (MQ=255)
                                                                                                ccTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGttt                                                       <  2:287653/150‑1 (MQ=255)
                                                                                                  ttCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCg                                                     <  2:967942/150‑1 (MQ=255)
                                                                                                   tCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAACCCTTAAGTTTCGa                                                    <  1:1215432/150‑1 (MQ=255)
                                                                                                    ccGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAg                                                   <  2:1654545/150‑1 (MQ=255)
                                                                                                    ccGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAg                                                   <  1:1472264/150‑1 (MQ=255)
                                                                                                         aCTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAgccgcc                                              <  1:4629095/150‑1 (MQ=255)
                                                                                                           tCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTc                                            <  1:2072057/150‑1 (MQ=255)
                                                                                                             aTTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTcac                                          <  1:3656923/150‑1 (MQ=255)
                                                                                                             aTTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTcac                                          >  1:3324632/1‑150 (MQ=255)
                                                                                                             aTTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTcac                                          >  2:63625/1‑150 (MQ=255)
                                                                                                              ttATCATAATTATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTcaca                                         <  2:1544210/150‑1 (MQ=255)
                                                                                                                 tCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACAttt                                      >  1:1787669/1‑150 (MQ=255)
                                                                                                                 tCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACAttt                                      <  1:394617/150‑1 (MQ=255)
                                                                                                                    tAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACAtttttt                                   <  1:853639/150‑1 (MQ=255)
                                                                                                                      aTGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTta                                 <  1:1406096/150‑1 (MQ=255)
                                                                                                                        gATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTtata                               <  2:3098729/150‑1 (MQ=255)
                                                                                                                          tAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATAtt                             <  2:1001909/150‑1 (MQ=255)
                                                                                                                           aaGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATAttt                            >  1:1885170/1‑150 (MQ=255)
                                                                                                                              gAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTccc                         >  1:4476614/1‑150 (MQ=255)
                                                                                                                              gAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTccc                         <  1:3591771/150‑1 (MQ=255)
                                                                                                                               aaTCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAACCGCCTCACATTTTTTTATATTTCCCg                        >  2:3569815/1‑150 (MQ=255)
                                                                                                                                 tCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGcc                      >  1:1005209/1‑150 (MQ=255)
                                                                                                                                 tCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGcc                      >  2:3850566/1‑150 (MQ=255)
                                                                                                                                  cACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCa                     <  1:3717008/150‑1 (MQ=255)
                                                                                                                                    cTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCaaa                   >  2:3175113/1‑150 (MQ=255)
                                                                                                                                    cTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCaaa                   >  2:2565041/1‑150 (MQ=255)
                                                                                                                                    cTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTGTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCaaa                   >  2:4442619/1‑150 (MQ=255)
                                                                                                                                     tGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAAc                  >  1:1626245/1‑150 (MQ=255)
                                                                                                                                       aTTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCt                <  1:4598899/150‑1 (MQ=255)
                                                                                                                                         tttGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTgg              >  1:1744549/1‑150 (MQ=255)
                                                                                                                                          ttGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGc             >  2:2892492/1‑150 (MQ=255)
                                                                                                                                          ttGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGc             >  1:1641245/1‑150 (MQ=255)
                                                                                                                                            gagaaaagataaaaATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCac           >  2:1421052/1‑149 (MQ=255)
                                                                                                                                            gagaaaagagaaaaATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCaa           >  2:3463046/1‑150 (MQ=255)
                                                                                                                                              gaaaagagaaaaATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAaga         <  1:3067894/150‑1 (MQ=255)
                                                                                                                                               aaaagagaaaaATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAagag        >  2:4055659/1‑150 (MQ=255)
                                                                                                                                                     gaaaaATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAAGAGTGGTGc  >  2:2365210/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAAGAGTGGTGC  >  CP009273/3009038‑3009336

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A