Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F204 I0 R1	1155	255.8	8356456	95.7%	7997128	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,009,187	G→T	52.3%	intergenic (+90/‑232)	mocA → / → ygfK	CTP:molybdopterin cytidylyltransferase/putative Fe‑S subunit oxidoreductase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,009,187	0	G	T	52.3%	29.2 / inf	243	intergenic (+90/‑232)	mocA/ygfK	CTP:molybdopterin cytidylyltransferase/putative Fe‑S subunit oxidoreductase subunit
Reads supporting (aligned to +/- strand): ref base G (57/59); new base T (60/67); total (117/126)
Fisher's exact test for biased strand distribution p-value = 7.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 262 total aligned reads displayed.

AGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAAGAGTGGTG  >  CP009273/3009040‑3009335

  atatTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTgagaaaagagaaaa                                                                                                                                                  >  1:3614584/1‑150 (MQ=255)

    atTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAAt                                                                                                                                                >  1:1023439/1‑150 (MQ=255)

    atTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAAt                                                                                                                                                >  1:883511/1‑150 (MQ=255)

    atTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAAt                                                                                                                                                >  2:2341269/1‑150 (MQ=255)

    atTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAAt                                                                                                                                                <  1:3343024/150‑1 (MQ=255)

    atTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAAt                                                                                                                                                >  2:19049/1‑150 (MQ=255)

      tGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTg                                                                                                                                              >  2:3914266/1‑150 (MQ=255)

      tGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTg                                                                                                                                              >  2:256328/1‑150 (MQ=255)

      tGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTg                                                                                                                                              >  2:3148053/1‑150 (MQ=255)

         tACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATa                                                                                                                                           <  1:794832/150‑1 (MQ=255)

         tACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATa                                                                                                                                           >  1:3153211/1‑150 (MQ=255)

           cTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATaaa                                                                                                                                         <  2:1818498/150‑1 (MQ=255)

              cGGACGACTTTATTACAGCGAAGGAAAGTTATACTGAAATTAAGAAAAAGAAGTAAAACGATTGCGGTCAAATATTTAATCATTCCGGCACTCATAATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATgt                                                                                                                                      >  2:1842105/1‑149 (MQ=255)

              cGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATgg                                                                                                                                      >  1:1338238/1‑150 (MQ=255)

              cGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATgg                                                                                                                                      <  1:119680/150‑1 (MQ=255)

              cGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATgg                                                                                                                                      >  1:3303826/1‑150 (MQ=255)

               ggACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATggg                                                                                                                                     <  2:52324/150‑1 (MQ=255)

                 acgacTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCt                                                                                                                                   <  1:2909309/150‑1 (MQ=255)

                  cgacTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTg                                                                                                                                  <  1:3478926/150‑1 (MQ=255)

                  cgacTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTg                                                                                                                                  <  1:3190672/150‑1 (MQ=255)

                   gacTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTgg                                                                                                                                 >  2:2701323/1‑150 (MQ=255)

                    acTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGc                                                                                                                                <  2:2907107/150‑1 (MQ=255)

                     cTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCa                                                                                                                               <  2:3763023/150‑1 (MQ=255)

                        tattaCAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCaata                                                                                                                            >  2:1761349/1‑150 (MQ=255)

                        tattaCAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCaata                                                                                                                            <  1:2500148/150‑1 (MQ=255)

                          ttaCAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATACTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCaataat                                                                                                                          >  2:4128338/1‑150 (MQ=255)

                           taCAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCaataata                                                                                                                         <  1:1862793/150‑1 (MQ=255)

                           taCAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCaataata                                                                                                                         <  2:3864838/150‑1 (MQ=255)

                              aGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAAtt                                                                                                                      <  1:815874/150‑1 (MQ=255)

                                cGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAAtttt                                                                                                                    <  1:3951858/150‑1 (MQ=255)

                                 gAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTc                                                                                                                   >  1:4111395/1‑150 (MQ=255)

                                   aGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAg                                                                                                                 >  2:684034/1‑150 (MQ=255)

                                    ggAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAgg                                                                                                                >  2:1617462/1‑150 (MQ=255)

                                     gAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGa                                                                                                               >  2:794334/1‑150 (MQ=255)

                                           tataCTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGAtt                                                                                                         <  2:3428074/150‑1 (MQ=255)

                                            ataCTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGAttt                                                                                                        <  2:3626371/150‑1 (MQ=255)

                                             taCTTAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGAtttt                                                                                                       <  2:3302213/150‑1 (MQ=255)

                                               cTGAAATTTATAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTaa                                                                                                     <  1:2816923/150‑1 (MQ=255)

                                                        aGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATTGGCTGGCAATAATAATTTACAGGAGTGATTTTAATTCTCAttt                                                                                            >  1:1045026/1‑150 (MQ=255)

                                                          aaaaCGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAg                                                                                          >  2:1252377/1‑150 (MQ=255)

                                                          aaaaCGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAg                                                                                          <  2:3446626/150‑1 (MQ=255)

                                                            aaCGTAGTTAAACGATTGCGTTCAAATATTAAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCa                                                                                        >  1:3862434/1‑150 (MQ=255)

                                                               gTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCt                                                                                     <  1:2840734/150‑1 (MQ=255)

                                                               gTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCt                                                                                     <  1:2060847/150‑1 (MQ=255)

                                                               gTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCt                                                                                     <  2:3192002/150‑1 (MQ=255)

                                                                   ttAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGa                                                                                 <  1:1782359/150‑1 (MQ=255)

                                                                   ttAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGa                                                                                 <  1:2319580/150‑1 (MQ=255)

                                                                    tAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAg                                                                                <  2:2001807/150‑1 (MQ=255)

                                                                    tAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAg                                                                                <  1:1830514/150‑1 (MQ=255)

                                                                      aaCGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGtt                                                                              >  2:2318653/1‑150 (MQ=255)

                                                                        cGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGttca                                                                            >  2:582904/1‑150 (MQ=255)

                                                                         gATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTATCACCTGTGAGttcat                                                                           >  1:3787140/1‑150 (MQ=255)

                                                                           ttGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGttcattc                                                                         <  2:3668186/150‑1 (MQ=255)

                                                                           ttGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGttcattc                                                                         >  2:2748697/1‑150 (MQ=255)

                                                                             gCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTcac                                                                       <  2:870561/150‑1 (MQ=255)

                                                                              cGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTcaca                                                                      <  2:104947/150‑1 (MQ=255)

                                                                               gTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACATGTGAGTTCATTCACaa                                                                     >  1:2087359/1‑150 (MQ=255)

                                                                                  cAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACaaaaa                                                                  >  1:3686713/1‑150 (MQ=255)

                                                                                    aaTATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGc                                                                <  2:2307054/150‑1 (MQ=255)

                                                                                    aaTATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGc                                                                <  2:2610461/150‑1 (MQ=255)

                                                                                     atatTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGcc                                                               >  1:286658/1‑150 (MQ=255)

                                                                                          tAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTaa                                                          >  1:213913/1‑150 (MQ=255)

                                                                                             tCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGtt                                                       <  1:3504933/150‑1 (MQ=255)

                                                                                             tCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGtt                                                       <  2:956154/150‑1 (MQ=255)

                                                                                              ccTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGttt                                                      <  1:910888/150‑1 (MQ=255)

                                                                                               cTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTc                                                     <  2:3291772/150‑1 (MQ=255)

                                                                                               cTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTc                                                     <  2:2953776/150‑1 (MQ=255)

                                                                                               cTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTc                                                     <  1:936570/150‑1 (MQ=255)

                                                                                                ttCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCg                                                    >  1:1465862/1‑150 (MQ=255)

                                                                                                   cGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAgc                                                 >  1:4021751/1‑150 (MQ=255)

                                                                                                     gCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAgccg                                               >  1:1698521/1‑150 (MQ=255)

                                                                                                      cACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAgccgc                                              >  2:1978591/1‑150 (MQ=255)

                                                                                                          cATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTca                                          >  2:1033747/1‑150 (MQ=255)

                                                                                                            ttATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTcaca                                        >  1:681951/1‑150 (MQ=255)

                                                                                                             tATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACAt                                       <  2:2369043/150‑1 (MQ=255)

                                                                                                               tCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACAttt                                     <  2:2513815/150‑1 (MQ=255)

                                                                                                                  tAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACAtttttt                                  <  2:3980553/150‑1 (MQ=255)

                                                                                                                    aTGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTta                                <  1:4170813/150‑1 (MQ=255)

                                                                                                                     tGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTtat                               <  1:1231131/150‑1 (MQ=255)

                                                                                                                     tGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTtat                               <  1:1881935/150‑1 (MQ=255)

                                                                                                                     tGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGAACCTGTGAGTTCATTCACAAAAAGCCATTAAGTTTCGAGCCGCCTCACATTTTTTtat                               >  2:1791793/1‑150 (MQ=255)

                                                                                                                      gATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTtata                              >  1:2543138/1‑150 (MQ=255)

                                                                                                                         aaGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATAttt                           <  2:4053179/150‑1 (MQ=255)

                                                                                                                            gAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTccc                        >  1:3061363/1‑150 (MQ=255)

                                                                                                                             aaTCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCg                       <  2:151974/150‑1 (MQ=255)

                                                                                                                                    gATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGACCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAAcc                >  2:3359201/1‑150 (MQ=255)

                                                                                                                                    gATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAAcc                <  2:383755/150‑1 (MQ=255)

                                                                                                                                    gATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAAcc                <  2:3477653/150‑1 (MQ=255)

                                                                                                                                    gATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAAcc                >  1:2995201/1‑150 (MQ=255)

                                                                                                                                    gATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAAcc                >  1:3754359/1‑150 (MQ=255)

                                                                                                                                    gATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAAGTTTCAGGAGTGATTTTAATTCTCATTTATCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCTAGCCGCCTCACATTTTTTTATATTTCCCGCCAAAcc                >  2:2821730/1‑150 (MQ=255)

                                                                                                                                      ttttGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTg              <  1:2322946/150‑1 (MQ=255)

                                                                                                                                       tttGAGAAAATATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGATCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTgg             <  2:3157168/150‑1 (MQ=255)

                                                                                                                                         tGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCa           <  2:2089827/150‑1 (MQ=255)

                                                                                                                                         tGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCa           >  1:1179073/1‑150 (MQ=255)

                                                                                                                                          gagaaaagataaaaATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCaa          >  1:501156/1‑150 (MQ=255)

                                                                                                                                            gaaaagataaaaATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAaga        >  1:2696982/1‑150 (MQ=255)

                                                                                                                                             aaaagataaaaATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAagag       >  2:3591234/1‑150 (MQ=255)

                                                                                                                                               aagataaaaATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAAGAgtg     <  1:256222/150‑1 (MQ=255)

                                                                                                                                               aagataaaaATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACATTTGAGTTCATTCAAAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAAGAgtg     <  2:2129016/150‑1 (MQ=255)

AGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAAGAGTGGTG  >  CP009273/3009040‑3009335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAAGAGTGGTG  >  CP009273/3009040‑3009335

AGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAA                                                                                                                                                    >  SRR19973074.999273/1‑150 (MQ=60)

  ATATTGATACTCCGGACGACTTTATTACAGCGAAGTAAAGGTATACTGAAATTTATAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAA                                                                                                                                                  >  SRR19973074.3575357/1‑150 (MQ=60)

  ATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAA                                                                                                                                                  >  SRR19973074.3621259/1‑150 (MQ=60)

  ATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAA                                                                                                                                                  >  SRR19973074.448933/1‑150 (MQ=60)

    ATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAAT                                                                                                                                                >  SRR19973074.1025334/1‑150 (MQ=60)

    ATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAAT                                                                                                                                                >  SRR19973074.885209/1‑150 (MQ=60)

    ATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAAT                                                                                                                                                <  SRR19973074.3349230/150‑1 (MQ=60)

    ATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAAT                                                                                                                                                <  SRR19973074.3349188/150‑1 (MQ=60)

      TGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTG                                                                                                                                              >  SRR19973074.2785281/1‑150 (MQ=60)

         TACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATA                                                                                                                                           <  SRR19973074.796359/150‑1 (MQ=60)

         TACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATA                                                                                                                                           >  SRR19973074.3159009/1‑150 (MQ=60)

             CCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATG                                                                                                                                       <  SRR19973074.1329898/150‑1 (MQ=60)

              CGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGG                                                                                                                                      <  SRR19973074.119964/150‑1 (MQ=60)

              CGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGG                                                                                                                                      <  SRR19973074.120224/150‑1 (MQ=60)

              CGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGG                                                                                                                                      >  SRR19973074.1340739/1‑150 (MQ=60)

              CGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGG                                                                                                                                      <  SRR19973074.1443755/150‑1 (MQ=60)

              CGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGG                                                                                                                                      >  SRR19973074.3309950/1‑150 (MQ=60)

                 ACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCT                                                                                                                                   <  SRR19973074.2914199/150‑1 (MQ=60)

                 ACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCT                                                                                                                                   <  SRR19973074.2914713/150‑1 (MQ=60)

                  CGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTG                                                                                                                                  <  SRR19973074.3485413/150‑1 (MQ=60)

                  CGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTG                                                                                                                                  <  SRR19973074.3196549/150‑1 (MQ=60)

                  CGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTG                                                                                                                                  <  SRR19973074.3485313/150‑1 (MQ=60)

                  CGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTG                                                                                                                                  <  SRR19973074.77074/150‑1 (MQ=60)

                     CTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCA                                                                                                                               <  SRR19973074.3688644/150‑1 (MQ=60)

                     CTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCA                                                                                                                               <  SRR19973074.3770612/150‑1 (MQ=60)

                     CTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCA                                                                                                                               >  SRR19973074.2310585/1‑150 (MQ=60)

                      TTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAA                                                                                                                              >  SRR19973074.3714711/1‑150 (MQ=60)

                       TTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAAT                                                                                                                             >  SRR19973074.333813/1‑150 (MQ=60)

                        TATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATA                                                                                                                            <  SRR19973074.2504801/150‑1 (MQ=60)

                        TATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATA                                                                                                                            <  SRR19973074.2504761/150‑1 (MQ=60)

                         ATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAA                                                                                                                           <  SRR19973074.3267196/150‑1 (MQ=60)

                           TACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATA                                                                                                                         >  SRR19973074.2920299/1‑150 (MQ=60)

                           TACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATA                                                                                                                         >  SRR19973074.2919943/1‑150 (MQ=60)

                           TACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATA                                                                                                                         <  SRR19973074.1866353/150‑1 (MQ=60)

                           TACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATA                                                                                                                         >  SRR19973074.574069/1‑150 (MQ=60)

                              AGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATT                                                                                                                      <  SRR19973074.817445/150‑1 (MQ=60)

                              AGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATT                                                                                                                      <  SRR19973074.817370/150‑1 (MQ=60)

                                CGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTT                                                                                                                    <  SRR19973074.3959138/150‑1 (MQ=60)

                                 GAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTC                                                                                                                   >  SRR19973074.4118988/1‑150 (MQ=60)

                                       AAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGT                                                                                                             >  SRR19973074.1734248/1‑150 (MQ=60)

                                           TATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATT                                                                                                         >  SRR19973074.364284/1‑150 (MQ=60)

                                           TATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATT                                                                                                         >  SRR19973074.444885/1‑150 (MQ=60)

                                               CTGAAATTTATAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAA                                                                                                     <  SRR19973074.2822132/150‑1 (MQ=60)

                                               CTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAA                                                                                                     <  SRR19973074.2823166/150‑1 (MQ=60)

                                                TGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAAT                                                                                                    <  SRR19973074.2240450/150‑1 (MQ=60)

                                                  AAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTC                                                                                                  >  SRR19973074.1873209/1‑150 (MQ=60)

                                                     TTTAGAATACGTTGTGAAACGATTGCTTTCTAATATTTACTCCTTCCGGCACTCATTATCATAATGATTAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCA                                                                                               <  SRR19973074.4028902/150‑1 (MQ=60)

                                                      TTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCAT                                                                                              >  SRR19973074.2565237/1‑150 (MQ=60)

                                                       TAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAAGTCTCATT                                                                                             >  SRR19973074.1822238/1‑150 (MQ=60)

                                                        AGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTT                                                                                            >  SRR19973074.2528868/1‑150 (MQ=60)

                                                        AGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATTGGCTGGCAATAATAATTTACAGGAGTGATTTTAATTCTCATTT                                                                                            >  SRR19973074.1046948/1‑150 (MQ=60)

                                                          AAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAG                                                                                          >  SRR19973074.3913168/1‑150 (MQ=60)

                                                            AACGTAGTTAAACGATTGCGTTCAAATATTAAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCA                                                                                        >  SRR19973074.3869485/1‑150 (MQ=60)

                                                               GTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCT                                                                                     <  SRR19973074.2064717/150‑1 (MQ=60)

                                                               GTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCT                                                                                     <  SRR19973074.2845994/150‑1 (MQ=60)

                                                               GTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCT                                                                                     <  SRR19973074.2846013/150‑1 (MQ=60)

                                                                  GTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTG                                                                                  >  SRR19973074.2331130/1‑150 (MQ=60)

                                                                   TTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGA                                                                                 <  SRR19973074.1785727/150‑1 (MQ=60)

                                                                   TTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGA                                                                                 <  SRR19973074.1785728/150‑1 (MQ=60)

                                                                   TTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGA                                                                                 <  SRR19973074.970962/150‑1 (MQ=60)

                                                                   TTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGA                                                                                 <  SRR19973074.2323914/150‑1 (MQ=60)

                                                                    TAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAG                                                                                <  SRR19973074.1834079/150‑1 (MQ=60)

                                                                    TAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAG                                                                                <  SRR19973074.1834007/150‑1 (MQ=60)

                                                                     AAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGT                                                                               <  SRR19973074.2864741/150‑1 (MQ=60)

                                                                      AACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTT                                                                              >  SRR19973074.1068055/1‑150 (MQ=60)

                                                                      AACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTT                                                                              >  SRR19973074.873237/1‑150 (MQ=60)

                                                                       ACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTC                                                                             <  SRR19973074.1067860/150‑1 (MQ=60)

                                                                         GATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTATCACCTGTGAGTTCAT                                                                           >  SRR19973074.3794081/1‑150 (MQ=60)

                                                                         GATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCAT                                                                           >  SRR19973074.3392952/1‑150 (MQ=60)

                                                                          ATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATT                                                                          >  SRR19973074.4148496/1‑150 (MQ=60)

                                                                             GCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCAC                                                                       <  SRR19973074.1248771/150‑1 (MQ=60)

                                                                               GTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAA                                                                     >  SRR19973074.2091120/1‑150 (MQ=60)

                                                                               GTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAA                                                                     >  SRR19973074.2090977/1‑150 (MQ=60)

                                                                               GTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACATGTGAGTTCATTCACAA                                                                     >  SRR19973074.2091267/1‑150 (MQ=60)

                                                                                 TCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAAGAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAA                                                                   >  SRR19973074.2098694/1‑150 (MQ=60)

                                                                                  CAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAA                                                                  >  SRR19973074.3693529/1‑150 (MQ=60)

                                                                                     ATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCC                                                               >  SRR19973074.287198/1‑150 (MQ=60)

                                                                                      TATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCC                                                              <  SRR19973074.3793273/150‑1 (MQ=60)

                                                                                       ATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAATTTGATAAATGGGCTGGCAATAATAATTTTCTTGAGTGATGTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCT                                                             >  SRR19973074.3600915/1‑150 (MQ=60)

                                                                                          TAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAA                                                          >  SRR19973074.214354/1‑150 (MQ=60)

                                                                                           AATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAG                                                         >  SRR19973074.3046132/1‑150 (MQ=60)

                                                                                            ATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGT                                                        <  SRR19973074.1610491/150‑1 (MQ=60)

                                                                                             TCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTT                                                       >  SRR19973074.3374952/1‑150 (MQ=60)

                                                                                             TCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTT                                                       <  SRR19973074.3511369/150‑1 (MQ=60)

                                                                                              CCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTT                                                      <  SRR19973074.912623/150‑1 (MQ=60)

                                                                                              CCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTT                                                      <  SRR19973074.912753/150‑1 (MQ=60)

                                                                                               CTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTC                                                     <  SRR19973074.938353/150‑1 (MQ=60)

                                                                                               CTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTC                                                     <  SRR19973074.3173981/150‑1 (MQ=60)

                                                                                                TTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCG                                                    <  SRR19973074.851949/150‑1 (MQ=60)

                                                                                                TTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCG                                                    >  SRR19973074.1468613/1‑150 (MQ=60)

                                                                                                   CGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGC                                                 >  SRR19973074.4029184/1‑150 (MQ=60)

                                                                                                    GGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCC                                                >  SRR19973074.1838913/1‑150 (MQ=60)

                                                                                                    GGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCC                                                >  SRR19973074.1028257/1‑150 (MQ=60)

                                                                                                     GCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCG                                               >  SRR19973074.3178593/1‑150 (MQ=60)

                                                                                                     GCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCG                                               >  SRR19973074.73115/1‑150 (MQ=60)

                                                                                                     GCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCG                                               >  SRR19973074.1701692/1‑150 (MQ=60)

                                                                                                     GCACTCATTAACATAATGATAAGGAATCACAGATTATGAGAAAATATAAAAATTGATAAATGGGCTGGAAATAATAATTTTCATTAGTGATTTAAAGTATAATTTAGCACATGTGAGTAAATTAAAAAAAAGCCCTTAAGTTacaaccca                                               >  SRR19973074.1701630/1‑142 (MQ=50)

                                                                                                          CATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCA                                          <  SRR19973074.1659639/150‑1 (MQ=60)

                                                                                                            TTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACA                                        >  SRR19973074.683205/1‑150 (MQ=60)

                                                                                                               TCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTT                                     >  SRR19973074.4080684/1‑150 (MQ=60)

                                                                                                                    ATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTA                                <  SRR19973074.4178477/150‑1 (MQ=60)

                                                                                                                     TGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTAT                               <  SRR19973074.1885536/150‑1 (MQ=60)

                                                                                                                     TGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTAT                               <  SRR19973074.1233489/150‑1 (MQ=60)

                                                                                                                     TGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTAT                               <  SRR19973074.1233468/150‑1 (MQ=60)

                                                                                                                      GATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATA                              >  SRR19973074.2547884/1‑150 (MQ=60)

                                                                                                                      GATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATA                              <  SRR19973074.4156126/150‑1 (MQ=60)

                                                                                                                       ATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATAT                             >  SRR19973074.2040239/1‑150 (MQ=60)

                                                                                                                           GGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCC                         >  SRR19973074.88812/1‑150 (MQ=60)

                                                                                                                            GAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCC                        >  SRR19973074.3067009/1‑150 (MQ=60)

                                                                                                                            GAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCC                        >  SRR19973074.3066754/1‑150 (MQ=60)

                                                                                                                                    GATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACC                <  SRR19973074.890659/150‑1 (MQ=60)

                                                                                                                                    GATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACC                >  SRR19973074.3000763/1‑150 (MQ=60)

                                                                                                                                    GATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACC                >  SRR19973074.3846130/1‑150 (MQ=60)

                                                                                                                                    GATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACC                >  SRR19973074.3761258/1‑150 (MQ=60)

                                                                                                                                     ATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCT               <  SRR19973074.2516565/150‑1 (MQ=60)

                                                                                                                                     ATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCT               >  SRR19973074.1940666/1‑150 (MQ=60)

                                                                                                                                     ATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCT               >  SRR19973074.3940406/1‑150 (MQ=60)

                                                                                                                                      TTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTG              <  SRR19973074.2327285/150‑1 (MQ=60)

                                                                                                                                      TTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTG              <  SRR19973074.2327283/150‑1 (MQ=60)

                                                                                                                                         TGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCA           >  SRR19973074.1181320/1‑150 (MQ=60)

                                                                                                                                         TGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCA           >  SRR19973074.1181318/1‑150 (MQ=60)

                                                                                                                                          GAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAA          >  SRR19973074.502159/1‑150 (MQ=60)

                                                                                                                                            GAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAAGA        >  SRR19973074.2701929/1‑150 (MQ=60)

                                                                                                                                            GAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAAGA        >  SRR19973074.2701990/1‑150 (MQ=60)

                                                                                                                                               AAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCTCCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAAGAGTG     <  SRR19973074.256837/150‑1 (MQ=60)

                                                                                                                                               AAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAAGAGTG     <  SRR19973074.256729/150‑1 (MQ=60)

                                                                                                                                                AGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAAGAGTGG    >  SRR19973074.3113531/1‑150 (MQ=60)

                                                                                                                                                 GATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAAGAGTGGT   <  SRR19973074.4156346/150‑1 (MQ=60)

                                                                                                                                                 GAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAAGAGTGGT   <  SRR19973074.3979415/150‑1 (MQ=60)

                                                                                                                                                  AGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAAGAGTGGTG  >  SRR19973074.2774271/1‑150 (MQ=60)

AGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAAGAGTGGTG  >  CP009273/3009040‑3009335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`