Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,009,187 G→T 100% intergenic (+90/‑232) mocA → / → ygfK CTP:molybdopterin cytidylyltransferase/putative Fe‑S subunit oxidoreductase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,009,1870GT98.4% 599.0 / 0.0 185intergenic (+90/‑232)mocA/ygfKCTP:molybdopterin cytidylyltransferase/putative Fe‑S subunit oxidoreductase subunit
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base T (103/79);  total (105/80)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 205 total aligned reads displayed.

AGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAAGAGTG  >  CP009273/3009040‑3009332
                                                                                                                                                   |                                                                                                                                                 
aGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTgagaaaagataa                                                                                                                                                 >  2:3094073/1‑150 (MQ=255)
aGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGAGTTTgagaaaagataa                                                                                                                                                 >  1:4820328/1‑150 (MQ=255)
 gATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTgagaaaagataaa                                                                                                                                                <  1:4235708/150‑1 (MQ=255)
  atatTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTgagaaaagataaaa                                                                                                                                               <  1:1621730/150‑1 (MQ=255)
  atatTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGAGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTgagaaaagataaaa                                                                                                                                               <  1:2122559/150‑1 (MQ=255)
   tatTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTAAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATaaaaa                                                                                                                                              >  1:5014992/1‑150 (MQ=255)
    atTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAAt                                                                                                                                             >  1:978504/1‑150 (MQ=255)
      ggATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTg                                                                                                                                           >  2:1256519/2‑150 (MQ=255)
         tACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATa                                                                                                                                        >  2:3280817/1‑150 (MQ=255)
          aCTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATaa                                                                                                                                       >  1:1085081/1‑150 (MQ=255)
          aCTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATaa                                                                                                                                       >  2:1635643/1‑150 (MQ=255)
              cGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATgg                                                                                                                                   >  2:4287855/1‑150 (MQ=255)
              cGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATgg                                                                                                                                   <  2:885137/150‑1 (MQ=255)
                 acgacTTTATTACAGCGAAGGAAAGTTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCt                                                                                                                                <  2:3898493/150‑1 (MQ=255)
                 acgacTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCt                                                                                                                                <  2:2193944/150‑1 (MQ=255)
                   gagttttttACAGCGAAGTAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCTTCACTCATTATCATAATGATTATGAATCACTGATTTTGAGAATAGATAAAAATTGATAAATGGGCTgg                                                                                                                              <  2:1390789/147‑1 (MQ=255)
                   gacTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTgg                                                                                                                              >  1:2772833/1‑150 (MQ=255)
                   gacTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTgg                                                                                                                              >  1:3611450/1‑150 (MQ=255)
                   gacTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTgg                                                                                                                              <  2:4065086/150‑1 (MQ=255)
                    acTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGc                                                                                                                             <  1:2307319/150‑1 (MQ=255)
                    acTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGc                                                                                                                             <  1:1705752/150‑1 (MQ=255)
                     cTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCa                                                                                                                            >  1:4258056/1‑150 (MQ=255)
                         attaCAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCaataa                                                                                                                        >  1:841975/1‑150 (MQ=255)
                          ttaCAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCaataat                                                                                                                       >  1:2452021/1‑150 (MQ=255)
                          ttaCAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCaataat                                                                                                                       <  1:574196/150‑1 (MQ=255)
                          ttaCAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCaataat                                                                                                                       >  2:2476228/1‑150 (MQ=255)
                          ttaCAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCaataat                                                                                                                       <  2:4271049/150‑1 (MQ=255)
                            aCAGCGAAGGAAAGGAATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCaataataa                                                                                                                     >  2:4995732/1‑150 (MQ=255)
                              aGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAAtt                                                                                                                   >  2:608416/1‑150 (MQ=255)
                              aGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAAtt                                                                                                                   >  1:4117278/1‑150 (MQ=255)
                                cGAAGGAAAGGTATACTGACATTTAGAAAGCGTAGTTAAACGATTGCGTTCGCATATTTAGTCCTTCCGGCACTCATTATCATAATCAGATGGACTCACTGAGGTTGAGACACGATACATCTGGATAAATGGGCTCGCCAGAATAAttag                                                                                                                 >  2:2679053/1‑148 (MQ=255)
                                 gAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTc                                                                                                                >  2:681474/1‑150 (MQ=255)
                                 gAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTc                                                                                                                >  2:4816459/1‑150 (MQ=255)
                                  aaGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCa                                                                                                               >  2:1718014/1‑150 (MQ=255)
                                   aGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTctg                                                                                                              >  1:2948974/1‑148 (MQ=255)
                                   aGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAg                                                                                                              >  2:2579750/1‑150 (MQ=255)
                                   aGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAg                                                                                                              <  2:2831023/150‑1 (MQ=255)
                                       aaGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGt                                                                                                          <  1:4370488/150‑1 (MQ=255)
                                          gTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGAt                                                                                                       >  2:4080624/1‑150 (MQ=255)
                                            ataCTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGAttt                                                                                                     <  2:4927339/150‑1 (MQ=255)
                                            ataCTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGAttt                                                                                                     <  2:4927172/150‑1 (MQ=255)
                                            ataCTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGAttt                                                                                                     <  1:1830264/150‑1 (MQ=255)
                                            ataCTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGAttt                                                                                                     >  2:2050556/1‑150 (MQ=255)
                                               cTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTaa                                                                                                  >  2:4229702/1‑150 (MQ=255)
                                                tGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAAt                                                                                                 >  2:4858974/1‑150 (MQ=255)
                                                 gAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAAtt                                                                                                >  1:2122211/1‑150 (MQ=255)
                                                 gAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAAtt                                                                                                >  1:3115427/1‑150 (MQ=255)
                                                           aaaCGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGc                                                                                      >  2:3905190/1‑150 (MQ=255)
                                                           aaaCGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGc                                                                                      >  1:415540/1‑150 (MQ=255)
                                                               gTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCt                                                                                  <  2:3629461/150‑1 (MQ=255)
                                                                tAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATCATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCtg                                                                                 >  2:4947730/1‑150 (MQ=255)
                                                                     aaaCGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAga                                                                            <  2:4105290/150‑2 (MQ=255)
                                                                       aCGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGttc                                                                          <  1:3964404/150‑1 (MQ=255)
                                                                         gATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGttcat                                                                        >  1:3073874/1‑150 (MQ=255)
                                                                         gATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGttcat                                                                        >  2:4465496/1‑150 (MQ=255)
                                                                         gATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGttcat                                                                        <  1:422093/150‑1 (MQ=255)
                                                                          aTTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGTCTGTCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGttcatt                                                                       >  1:471398/1‑150 (MQ=255)
                                                                          aTTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGttcatt                                                                       >  1:3471370/1‑150 (MQ=255)
                                                                          aTTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGttcatt                                                                       >  1:138429/1‑150 (MQ=255)
                                                                           ttGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGttcattc                                                                      <  2:841975/150‑1 (MQ=255)
                                                                           ttGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGttcattc                                                                      >  1:1269826/1‑150 (MQ=255)
                                                                            tGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGttcattca                                                                     <  1:3323344/150‑1 (MQ=255)
                                                                               gTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACaa                                                                  >  2:5129648/1‑150 (MQ=255)
                                                                               gTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACaa                                                                  >  1:1120241/1‑150 (MQ=255)
                                                                               gTTCAAATATTTAATCATTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACaa                                                                  <  2:2760748/150‑1 (MQ=255)
                                                                                   aaaTATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAg                                                              >  1:1916160/1‑150 (MQ=255)
                                                                                   aaaTATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAg                                                              <  2:159579/150‑1 (MQ=255)
                                                                                    aaTATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGc                                                             >  1:3808015/1‑150 (MQ=255)
                                                                                        tttAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCtt                                                         <  1:2163821/150‑1 (MQ=255)
                                                                                        tttAATCCTTCCGGATCTCATTATCATAATGTTAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCtt                                                         <  2:1128404/150‑1 (MQ=255)
                                                                                           aaTCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAg                                                      <  1:1982312/150‑1 (MQ=255)
                                                                                            aTCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGt                                                     <  1:4484123/150‑1 (MQ=255)
                                                                                            aTCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGt                                                     <  2:957432/150‑1 (MQ=255)
                                                                                              ccTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGttt                                                   <  1:1162777/150‑1 (MQ=255)
                                                                                              ccTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGttt                                                   <  2:4295380/150‑1 (MQ=255)
                                                                                                 tCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGa                                                <  2:4087105/150‑1 (MQ=255)
                                                                                                 tCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGa                                                >  2:2852889/1‑150 (MQ=255)
                                                                                                  ccGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAg                                               >  2:368310/1‑150 (MQ=255)
                                                                                                  ccGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAg                                               >  1:1562666/1‑150 (MQ=255)
                                                                                                    ggCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAgcc                                             >  2:3759079/1‑150 (MQ=255)
                                                                                                          cATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTca                                       <  2:3803872/150‑1 (MQ=255)
                                                                                                                cATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACAtttt                                 >  2:862107/1‑150 (MQ=255)
                                                                                                                 aTAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACAttttt                                >  1:4682311/1‑150 (MQ=255)
                                                                                                                          aGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTc                       >  2:5095494/1‑150 (MQ=255)
                                                                                                                          aGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTc                       >  1:2094767/1‑150 (MQ=255)
                                                                                                                           ggAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTcc                      <  1:3535415/150‑1 (MQ=255)
                                                                                                                            gAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTccc                     >  2:231314/1‑150 (MQ=255)
                                                                                                                             aaTCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCg                    <  2:1915131/150‑1 (MQ=255)
                                                                                                                                cACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCa                 <  1:987091/150‑1 (MQ=255)
                                                                                                                                  cTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCaaa               >  1:2420591/1‑150 (MQ=255)
                                                                                                                                  cTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCaaa               >  1:4408025/1‑150 (MQ=255)
                                                                                                                                   tGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAAc              >  1:1766276/1‑150 (MQ=255)
                                                                                                                                    gATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAAcc             >  2:3165160/1‑150 (MQ=255)
                                                                                                                                     aTTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCt            <  2:2413337/150‑1 (MQ=255)
                                                                                                                                        ttGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGc         <  2:2866783/150‑1 (MQ=255)
                                                                                                                                        ttGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGc         <  2:2118698/150‑1 (MQ=255)
                                                                                                                                         tGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCa        <  1:1718032/150‑1 (MQ=255)
                                                                                                                                          gagaaaagataaaaATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCaa       <  1:4627598/150‑1 (MQ=255)
                                                                                                                                              aaagatacaaATTGATAAATGGGCTGGCAATAATAATTTTCATGATTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAagaga   <  1:3275243/150‑2 (MQ=255)
                                                                                                                                              aaagataaaaATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAagaga   <  1:690324/150‑2 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
AGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAAGAGTG  >  CP009273/3009040‑3009332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAAGAGTGGT  >  CP009273/3009038‑3009334
                                                                                                                                                     |                                                                                                                                                   
TTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAT                                                                                                                                                     <  SRR19973063.2083574/150‑1 (MQ=60)
  AGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATCATGATAAGGACTCACTGATTTTGAGAAAAGATAA                                                                                                                                                   >  SRR19973063.4835762/1‑150 (MQ=60)
  AGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGAGTTTGAGAAAAGATAA                                                                                                                                                   >  SRR19973063.4835761/1‑150 (MQ=60)
   GATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAA                                                                                                                                                  <  SRR19973063.4249211/150‑1 (MQ=60)
   GATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAA                                                                                                                                                  <  SRR19973063.4249491/150‑1 (MQ=60)
    ATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAA                                                                                                                                                 <  SRR19973063.2129364/150‑1 (MQ=60)
    ATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAA                                                                                                                                                 <  SRR19973063.1626688/150‑1 (MQ=60)
    ATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGAGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAA                                                                                                                                                 <  SRR19973063.2129193/150‑1 (MQ=60)
     TATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTAAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAA                                                                                                                                                >  SRR19973063.5031181/1‑150 (MQ=60)
      ATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAAT                                                                                                                                               >  SRR19973063.960314/1‑150 (MQ=60)
      ATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAAT                                                                                                                                               >  SRR19973063.981455/1‑150 (MQ=60)
      ATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAAT                                                                                                                                               >  SRR19973063.981592/1‑150 (MQ=60)
        TGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTG                                                                                                                                             >  SRR19973063.2550463/1‑150 (MQ=60)
            ACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAA                                                                                                                                         >  SRR19973063.1088381/1‑150 (MQ=60)
                  GACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGC                                                                                                                                   >  SRR19973063.4345471/1‑150 (MQ=60)
                    CGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTG                                                                                                                                 <  SRR19973063.1660317/150‑1 (MQ=60)
                    CGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTG                                                                                                                                 <  SRR19973063.1983997/150‑1 (MQ=60)
                    CGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTG                                                                                                                                 >  SRR19973063.13074/1‑150 (MQ=60)
                     GACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGG                                                                                                                                >  SRR19973063.3622933/1‑150 (MQ=60)
                     GACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGG                                                                                                                                >  SRR19973063.3623046/1‑150 (MQ=60)
                     GACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGG                                                                                                                                >  SRR19973063.2421058/1‑150 (MQ=60)
                     GACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGG                                                                                                                                >  SRR19973063.2781770/1‑150 (MQ=60)
                      ACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGC                                                                                                                               <  SRR19973063.1710949/150‑1 (MQ=60)
                      ACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGC                                                                                                                               <  SRR19973063.2314695/150‑1 (MQ=60)
                       CTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCA                                                                                                                              >  SRR19973063.4271607/1‑150 (MQ=60)
                        TTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCTGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAAATTATAAATGGGCTGGCAA                                                                                                                             >  SRR19973063.7008/1‑150 (MQ=60)
                           ATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAA                                                                                                                          >  SRR19973063.844408/1‑150 (MQ=60)
                            TTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAAT                                                                                                                         >  SRR19973063.2459853/1‑150 (MQ=60)
                            TTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAAT                                                                                                                         <  SRR19973063.575891/150‑1 (MQ=60)
                            TTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAAT                                                                                                                         >  SRR19973063.2459769/1‑150 (MQ=60)
                            TTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAAT                                                                                                                         <  SRR19973063.469239/150‑1 (MQ=60)
                                AGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATT                                                                                                                     >  SRR19973063.4130462/1‑150 (MQ=60)
                                    AAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCA                                                                                                                 <  SRR19973063.1181861/150‑1 (MQ=60)
                                     AGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCTG                                                                                                                >  SRR19973063.2958400/1‑150 (MQ=60)
                                        AAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAG                                                                                                             <  SRR19973063.3525112/150‑1 (MQ=60)
                                         AAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGT                                                                                                            <  SRR19973063.4384471/150‑1 (MQ=60)
                                              ATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTT                                                                                                       <  SRR19973063.1835959/150‑1 (MQ=60)
                                               TACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTT                                                                                                      >  SRR19973063.4734213/1‑150 (MQ=60)
                                               TACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTT                                                                                                      >  SRR19973063.176962/1‑150 (MQ=60)
                                                 CTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAA                                                                                                    >  SRR19973063.3937344/1‑150 (MQ=60)
                                                   GAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATT                                                                                                  >  SRR19973063.2128845/1‑150 (MQ=60)
                                                   GAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATT                                                                                                  >  SRR19973063.3125455/1‑150 (MQ=60)
                                                   GAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATT                                                                                                  >  SRR19973063.2128993/1‑150 (MQ=60)
                                                            AAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAG                                                                                         >  SRR19973063.4558907/1‑150 (MQ=60)
                                                             AAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGC                                                                                        >  SRR19973063.416708/1‑150 (MQ=60)
                                                               ACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCAC                                                                                      >  SRR19973063.160074/1‑150 (MQ=60)
                                                                  TAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTG                                                                                   <  SRR19973063.2582518/150‑1 (MQ=60)
                                                                         ACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTC                                                                            <  SRR19973063.3977089/150‑1 (MQ=60)
                                                                           GATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCAT                                                                          >  SRR19973063.3083749/1‑150 (MQ=60)
                                                                           GATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCAT                                                                          >  SRR19973063.3084040/1‑150 (MQ=60)
                                                                           GATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCAT                                                                          <  SRR19973063.423273/150‑1 (MQ=60)
                                                                            ATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGTCTGTCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATT                                                                         >  SRR19973063.472727/1‑150 (MQ=60)
                                                                            ATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATT                                                                         >  SRR19973063.138864/1‑150 (MQ=60)
                                                                            ATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATT                                                                         >  SRR19973063.3482427/1‑150 (MQ=60)
                                                                            ATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATT                                                                         >  SRR19973063.3482431/1‑150 (MQ=60)
                                                                             TTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTC                                                                        >  SRR19973063.1273599/1‑150 (MQ=60)
                                                                              TGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCA                                                                       <  SRR19973063.3333954/150‑1 (MQ=60)
                                                                              TGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCA                                                                       <  SRR19973063.3333955/150‑1 (MQ=60)
                                                                                 GTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAA                                                                    >  SRR19973063.1123368/1‑150 (MQ=60)
                                                                                 GTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAA                                                                    >  SRR19973063.1123643/1‑150 (MQ=60)
                                                                                  TTCAAATATTTAATCCCTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAA                                                                   >  SRR19973063.4719780/1‑150 (MQ=60)
                                                                                   TCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAA                                                                  <  SRR19973063.4139859/150‑1 (MQ=60)
                                                                                   TCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAA                                                                  >  SRR19973063.1532185/1‑150 (MQ=60)
                                                                                     AAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAG                                                                >  SRR19973063.1922156/1‑150 (MQ=60)
                                                                                      AATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCTTTCACAAAAAGC                                                               >  SRR19973063.4422991/1‑150 (MQ=60)
                                                                                      AATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGC                                                               >  SRR19973063.3820139/1‑150 (MQ=60)
                                                                                       ATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCC                                                              >  SRR19973063.2112265/1‑150 (MQ=60)
                                                                                          TTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTT                                                           <  SRR19973063.2170598/150‑1 (MQ=60)
                                                                                             AATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAG                                                        <  SRR19973063.1988508/150‑1 (MQ=60)
                                                                                              ATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGT                                                       <  SRR19973063.4498501/150‑1 (MQ=60)
                                                                                                CCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTT                                                     <  SRR19973063.1166286/150‑1 (MQ=60)
                                                                                                CCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTT                                                     >  SRR19973063.3052049/1‑150 (MQ=60)
                                                                                                    CCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAG                                                 >  SRR19973063.1567467/1‑150 (MQ=60)
                                                                                                      GGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCC                                               <  SRR19973063.5090844/150‑1 (MQ=60)
                                                                                                      GGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCC                                               >  SRR19973063.1811697/1‑150 (MQ=60)
                                                                                                        CACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGC                                             >  SRR19973063.1893790/1‑150 (MQ=60)
                                                                                                                   ATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTT                                  >  SRR19973063.4697208/1‑150 (MQ=60)
                                                                                                                   ATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTT                                  >  SRR19973063.4697240/1‑150 (MQ=60)
                                                                                                                     AATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTT                                >  SRR19973063.1161737/1‑150 (MQ=60)
                                                                                                                      ATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTA                               <  SRR19973063.4395760/150‑1 (MQ=60)
                                                                                                                          TAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATT                           >  SRR19973063.659291/1‑150 (MQ=60)
                                                                                                                            AGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTC                         >  SRR19973063.2101314/1‑150 (MQ=60)
                                                                                                                             GGAATCACTTATTTTTAGAAAAGATAAAAATTGATAAATTGGCTTTCAATAATAATTTTCATGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCC                        <  SRR19973063.4196283/150‑1 (MQ=60)
                                                                                                                             GGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCC                        <  SRR19973063.3546744/150‑1 (MQ=60)
                                                                                                                              GAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCC                       >  SRR19973063.2304427/1‑150 (MQ=60)
                                                                                                                                  CACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCA                   <  SRR19973063.990067/150‑1 (MQ=60)
                                                                                                                                  CACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCA                   <  SRR19973063.4243180/150‑1 (MQ=60)
                                                                                                                                  CACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCA                   <  SRR19973063.209167/150‑1 (MQ=60)
                                                                                                                                    CTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAA                 >  SRR19973063.4422162/1‑150 (MQ=60)
                                                                                                                                    CTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAA                 >  SRR19973063.2428329/1‑150 (MQ=60)
                                                                                                                                     TGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAAC                >  SRR19973063.1771715/1‑150 (MQ=60)
                                                                                                                                        TTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAACAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTG             <  SRR19973063.2931159/150‑1 (MQ=60)
                                                                                                                                           TGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCA          <  SRR19973063.1723276/150‑1 (MQ=60)
                                                                                                                                           TGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCA          <  SRR19973063.1723258/150‑1 (MQ=60)
                                                                                                                                            GAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAA         <  SRR19973063.4642384/150‑1 (MQ=60)
                                                                                                                                             AGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAAG        <  SRR19973063.3277763/150‑1 (MQ=60)
                                                                                                                                               AAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAAGAG      <  SRR19973063.3654134/150‑1 (MQ=60)
                                                                                                                                                AAAGATACAAATTGATAAATGGGCTGGCAATAATAATTTTCATGATTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAAGAGA     <  SRR19973063.3285762/150‑1 (MQ=60)
                                                                                                                                                AAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAAGAGA     <  SRR19973063.692354/150‑1 (MQ=60)
                                                                                                                                                  AGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCACTTAAGTTTCGAGCCGCCTCACATTATTTTATATTTCACGCAAAACCTGTCAAGAGTTG   >  SRR19973063.4113771/1‑150 (MQ=60)
                                                                                                                                                   GATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAAGAGTGGT  >  SRR19973063.626404/1‑150 (MQ=60)
                                                                                                                                                   GATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAAGAGTGGT  >  SRR19973063.268985/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
TTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAAGAGTGGT  >  CP009273/3009038‑3009334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑