Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,009,187 G→T 100% intergenic (+90/‑232) mocA → / → ygfK CTP:molybdopterin cytidylyltransferase/putative Fe‑S subunit oxidoreductase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,009,1870GT98.8% 803.7 / 0.2 248intergenic (+90/‑232)mocA/ygfKCTP:molybdopterin cytidylyltransferase/putative Fe‑S subunit oxidoreductase subunit
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base T (120/125);  total (121/127)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 270 total aligned reads displayed.

ATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAAGAGT  >  CP009273/3009042‑3009331
                                                                                                                                                 |                                                                                                                                                
  atTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTNCGTTCAAGTATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTgagaaaagataaaa                                                                                                                                              >  1:1786190/1‑148 (MQ=255)
  atTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTgagaaaagataaaa                                                                                                                                              >  2:565147/1‑148 (MQ=255)
  atTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTgagaaaagataaaa                                                                                                                                              >  2:3070888/1‑148 (MQ=255)
  atTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTgagaaaagataaaa                                                                                                                                              >  2:2099015/1‑148 (MQ=255)
   ttGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATaaaaa                                                                                                                                             <  2:2220754/148‑1 (MQ=255)
    tGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAAt                                                                                                                                            <  1:458705/148‑1 (MQ=255)
      aTACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTg                                                                                                                                          >  2:216009/1‑148 (MQ=255)
       tACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGa                                                                                                                                         >  2:1186999/1‑148 (MQ=255)
       tACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGa                                                                                                                                         <  1:2155993/148‑1 (MQ=255)
         cTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATa                                                                                                                                       <  2:388727/148‑1 (MQ=255)
         cTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATa                                                                                                                                       >  2:150921/1‑148 (MQ=255)
           ccGGACGACTTTAGTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATaaa                                                                                                                                     <  1:1290527/148‑1 (MQ=255)
              gacgacTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATgg                                                                                                                                  >  2:2746629/1‑148 (MQ=255)
               acgacTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATggg                                                                                                                                 <  1:1380328/148‑1 (MQ=255)
                  acTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTg                                                                                                                              >  2:1904941/1‑148 (MQ=255)
                   cTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATTGGCTgg                                                                                                                             >  2:2855704/1‑148 (MQ=255)
                   cTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTgg                                                                                                                             >  2:2940492/1‑148 (MQ=255)
                   cTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTgg                                                                                                                             >  2:608279/1‑148 (MQ=255)
                     ttattaCAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCAATGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCa                                                                                                                           >  2:852969/1‑148 (MQ=255)
                         taCAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCaataa                                                                                                                       >  2:1054889/1‑148 (MQ=255)
                         taCAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCaataa                                                                                                                       >  1:686179/1‑148 (MQ=255)
                         taCAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCaataa                                                                                                                       >  1:1118600/1‑148 (MQ=255)
                             gCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCaataataat                                                                                                                   <  2:3277432/148‑1 (MQ=255)
                              cGAAGGAAAGTTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAAtt                                                                                                                  <  2:2993462/148‑1 (MQ=255)
                              cGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAAtt                                                                                                                  <  1:724593/148‑1 (MQ=255)
                              cGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAAtt                                                                                                                  <  2:1103033/148‑1 (MQ=255)
                              cGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAAtt                                                                                                                  <  1:3003975/148‑1 (MQ=255)
                                aaGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAAtttt                                                                                                                >  1:3401973/1‑148 (MQ=255)
                                aaGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGAGTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAAtttt                                                                                                                <  2:2310687/148‑1 (MQ=255)
                                 aGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTc                                                                                                               <  2:2435380/148‑1 (MQ=255)
                                  ggAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCa                                                                                                              >  2:1544053/1‑148 (MQ=255)
                                     aaGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGa                                                                                                           <  2:1877412/148‑1 (MQ=255)
                                      aGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAg                                                                                                          <  1:1595559/148‑1 (MQ=255)
                                       ggTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGt                                                                                                         <  1:105869/148‑1 (MQ=255)
                                         tataCTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGTGCTGGCAATAATAATTTTCAGGAGTGa                                                                                                       >  2:3503235/1‑148 (MQ=255)
                                         tataCTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGa                                                                                                       >  1:3210438/1‑148 (MQ=255)
                                              tGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTa                                                                                                  >  1:987986/1‑148 (MQ=255)
                                               gAAATTTAGAACACGTAGTTAAACGAGTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTaa                                                                                                 <  1:2075025/148‑1 (MQ=255)
                                               gAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTaa                                                                                                 >  2:3174458/1‑148 (MQ=255)
                                                     tAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCa                                                                                           <  2:2028756/148‑1 (MQ=255)
                                                       gAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCAtt                                                                                         >  2:1975544/1‑148 (MQ=255)
                                                       gAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCAtt                                                                                         >  2:2044658/1‑148 (MQ=255)
                                                        aaaaCGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCAttt                                                                                        >  2:3243811/1‑148 (MQ=255)
                                                         aaaCGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTa                                                                                       >  1:2218055/1‑148 (MQ=255)
                                                         aaaCGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTa                                                                                       <  1:1055799/148‑1 (MQ=255)
                                                          aaCGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAg                                                                                      >  1:1599836/1‑148 (MQ=255)
                                                          aaCGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAg                                                                                      >  2:2914017/1‑148 (MQ=255)
                                                            cGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCa                                                                                    <  2:3380673/148‑1 (MQ=255)
                                                             gTAGTTAAACCATTGCGATCAAATATATAATCCTTCCGGCAAACATTATCATCATGATAAGGAATCACTGATTTTGAGAAAAGATAAACATTGATACATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCAc                                                                                   <  1:1919502/148‑1 (MQ=255)
                                                               aGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCt                                                                                 >  1:3470163/1‑148 (MQ=255)
                                                                gTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCtg                                                                                <  1:2550706/148‑1 (MQ=255)
                                                                 ttAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCtgt                                                                               >  1:2672069/1‑148 (MQ=255)
                                                                 ttAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCtgt                                                                               >  2:1678952/1‑148 (MQ=255)
                                                                    aaCGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAg                                                                            <  1:2434920/148‑1 (MQ=255)
                                                                     aCGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGt                                                                           <  2:1146343/148‑1 (MQ=255)
                                                                     aCGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGt                                                                           <  1:2561165/148‑1 (MQ=255)
                                                                      cGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGtt                                                                          >  2:1765356/1‑148 (MQ=255)
                                                                        aTTGCGTTCAAATATTTAATCCTTCCGGCACTAATTATCATAATGATAAGGTATCACTGATTTTGAGAAAAGATAAAATTTGATAAATGGGCTGGCAATAATAATTTTCATGAGTGATTTTAATTCTCATTTAGCTCGTTTGAGttca                                                                        <  2:1720325/148‑1 (MQ=255)
                                                                                      tttAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGccc                                                          <  2:713362/148‑1 (MQ=255)
                                                                                      tttAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGccc                                                          <  1:544744/148‑1 (MQ=255)
                                                                                          aTCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTaa                                                      <  1:184804/148‑1 (MQ=255)
                                                                                          aTCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTaa                                                      >  1:3272829/1‑148 (MQ=255)
                                                                                              ttCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGttt                                                  <  2:1276949/148‑1 (MQ=255)
                                                                                              ttCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGttt                                                  <  1:3356347/148‑1 (MQ=255)
                                                                                                      cTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAgccgc                                          <  1:1937898/148‑1 (MQ=255)
                                                                                                       tCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAgccgcc                                         <  2:2533554/148‑1 (MQ=255)
                                                                                                        cATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCt                                        >  2:3446624/1‑148 (MQ=255)
                                                                                                             tCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACAt                                   <  1:1904941/148‑1 (MQ=255)
                                                                                                             tCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACAt                                   >  1:1186682/1‑148 (MQ=255)
                                                                                                              cATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACAtt                                  >  1:203348/1‑148 (MQ=255)
                                                                                                                   tGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACAttttttt                             >  2:963176/1‑148 (MQ=255)
                                                                                                                    gATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTta                            >  1:342786/1‑148 (MQ=255)
                                                                                                                     aTAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTtat                           >  1:3260313/1‑148 (MQ=255)
                                                                                                                      tAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATTAATGGGCTGGCAATTATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTtata                          <  1:2099015/148‑1 (MQ=255)
                                                                                                                      tAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTtata                          >  2:1675439/1‑148 (MQ=255)
                                                                                                                      tAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTtata                          <  2:686179/148‑1 (MQ=255)
                                                                                                                      tAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTtata                          <  2:3323026/148‑1 (MQ=255)
                                                                                                                      tAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTAAAGTTTCGAGCCGCCTCACATTTTTTtata                          >  2:3043315/1‑148 (MQ=255)
                                                                                                                        aGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATAtt                        >  2:3301503/1‑148 (MQ=255)
                                                                                                                         ggAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATAttt                       <  2:75149/148‑1 (MQ=255)
                                                                                                                         ggAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATAttt                       <  2:1851977/148‑1 (MQ=255)
                                                                                                                         ggAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATAttt                       <  1:3512122/148‑1 (MQ=255)
                                                                                                                          gAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTc                      <  1:2299440/148‑1 (MQ=255)
                                                                                                                          gAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTc                      >  1:848190/1‑148 (MQ=255)
                                                                                                                           aaTCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTcc                     <  1:3094079/148‑1 (MQ=255)
                                                                                                                             tCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCg                   >  2:2415302/1‑148 (MQ=255)
                                                                                                                              cACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGc                  >  1:2611091/1‑148 (MQ=255)
                                                                                                                               aCTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGcc                 >  1:1585721/1‑148 (MQ=255)
                                                                                                                                cTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCa                >  1:888460/1‑148 (MQ=255)
                                                                                                                                  gATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCaaa              <  2:3268138/148‑1 (MQ=255)
                                                                                                                                  gATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCaaa              <  2:1275481/148‑1 (MQ=255)
                                                                                                                                  gATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCaaa              <  2:1375213/148‑1 (MQ=255)
                                                                                                                                    ttttGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAAcc            <  1:2245872/148‑1 (MQ=255)
                                                                                                                                    ttttGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAAcc            >  1:1667255/1‑148 (MQ=255)
                                                                                                                                    ttttGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAAcc            >  2:2441019/1‑148 (MQ=255)
                                                                                                                                     tttGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCt           >  2:447799/1‑148 (MQ=255)
                                                                                                                                     tttGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCt           <  2:527861/148‑1 (MQ=255)
                                                                                                                                           aaaagataaaaATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAag     >  1:658200/1‑148 (MQ=255)
                                                                                                                                             aagataaaaATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAagag   <  1:150921/148‑1 (MQ=255)
                                                                                                                                              agataaaaATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAAGAgt  >  2:654848/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
ATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAAGAGT  >  CP009273/3009042‑3009331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAAGAGTGGTG  >  CP009273/3009038‑3009335
                                                                                                                                                     |                                                                                                                                                    
TTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAG                                                                                                                                                      <  SRR19973065.1702285/150‑1 (MQ=60)
 TAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATA                                                                                                                                                     >  SRR19973065.1602973/1‑150 (MQ=60)
  AGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAA                                                                                                                                                    <  SRR19973065.2947823/150‑1 (MQ=60)
    ATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTNCGTTCAAGTATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAA                                                                                                                                                  >  SRR19973065.1797879/1‑150 (MQ=60)
     TATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAA                                                                                                                                                 >  SRR19973065.2040484/1‑150 (MQ=60)
        TGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTG                                                                                                                                              >  SRR19973065.1114274/1‑150 (MQ=60)
        TGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTG                                                                                                                                              <  SRR19973065.461871/150‑1 (MQ=60)
         GATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGA                                                                                                                                             >  SRR19973065.629802/1‑150 (MQ=60)
         GATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGA                                                                                                                                             >  SRR19973065.628312/1‑150 (MQ=60)
          ATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGAT                                                                                                                                            <  SRR19973065.2566789/150‑1 (MQ=60)
          ATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGAT                                                                                                                                            >  SRR19973065.503265/1‑150 (MQ=60)
           TACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATA                                                                                                                                           <  SRR19973065.2169857/150‑1 (MQ=60)
            ACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAA                                                                                                                                          <  SRR19973065.1462870/150‑1 (MQ=60)
            ACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAA                                                                                                                                          <  SRR19973065.412083/150‑1 (MQ=60)
             CTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAA                                                                                                                                         <  SRR19973065.2703452/150‑1 (MQ=60)
               CCGGACGACTTTAGTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATG                                                                                                                                       <  SRR19973065.1299034/150‑1 (MQ=60)
                CGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGG                                                                                                                                      <  SRR19973065.689187/150‑1 (MQ=60)
                 GGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGG                                                                                                                                     >  SRR19973065.1802243/1‑150 (MQ=60)
                   ACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCT                                                                                                                                   <  SRR19973065.1389417/150‑1 (MQ=60)
                    CGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTG                                                                                                                                  >  SRR19973065.2019182/1‑150 (MQ=60)
                       ATTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCA                                                                                                                               <  SRR19973065.3037838/150‑1 (MQ=60)
                           ATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAA                                                                                                                           >  SRR19973065.690736/1‑150 (MQ=60)
                           ATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAA                                                                                                                           >  SRR19973065.3343303/1‑150 (MQ=60)
                           ATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAA                                                                                                                           >  SRR19973065.1125899/1‑150 (MQ=60)
                           ATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAA                                                                                                                           >  SRR19973065.1125838/1‑150 (MQ=60)
                           ATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTAGCATAATGTTAAGGACTCACTGATTTTGAGAAACGATAATAATTGATGAAGGGGCTGGCggtga                                                                                                                           >  SRR19973065.3343238/1‑145 (MQ=60)
                             TACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATA                                                                                                                         >  SRR19973065.3289052/1‑150 (MQ=60)
                               CAGCGAATGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAAT                                                                                                                       >  SRR19973065.2050834/1‑150 (MQ=60)
                               CAGCGAAGGAAAGGTATACTGATATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAAT                                                                                                                       <  SRR19973065.3456788/150‑1 (MQ=60)
                               CAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAAT                                                                                                                       <  SRR19973065.3457029/150‑1 (MQ=60)
                                AGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCACTAATAATT                                                                                                                      >  SRR19973065.2272887/1‑150 (MQ=60)
                                AGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATT                                                                                                                      >  SRR19973065.2272808/1‑150 (MQ=60)
                                AGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATT                                                                                                                      <  SRR19973065.3246084/150‑1 (MQ=60)
                                  CGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTT                                                                                                                    <  SRR19973065.959690/150‑1 (MQ=60)
                                  CGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTT                                                                                                                    <  SRR19973065.3022380/150‑1 (MQ=60)
                                  CGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTT                                                                                                                    >  SRR19973065.3422766/1‑150 (MQ=60)
                                  CGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTT                                                                                                                    >  SRR19973065.3494874/1‑150 (MQ=60)
                                  CGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTT                                                                                                                    <  SRR19973065.729443/150‑1 (MQ=60)
                                   GAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTC                                                                                                                   <  SRR19973065.3430782/150‑1 (MQ=60)
                                   GAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTC                                                                                                                   <  SRR19973065.758650/150‑1 (MQ=60)
                                     AGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAG                                                                                                                 <  SRR19973065.2351440/150‑1 (MQ=60)
                                       GAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGA                                                                                                               <  SRR19973065.1684262/150‑1 (MQ=60)
                                          AGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTG                                                                                                            <  SRR19973065.1606217/150‑1 (MQ=60)
                                          AGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTG                                                                                                            <  SRR19973065.1606135/150‑1 (MQ=60)
                                           GGTATACTTAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGA                                                                                                           <  SRR19973065.106820/150‑1 (MQ=60)
                                           GGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGA                                                                                                           >  SRR19973065.3229751/1‑150 (MQ=60)
                                           GGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGA                                                                                                           <  SRR19973065.2624539/150‑1 (MQ=60)
                                           GGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGA                                                                                                           <  SRR19973065.106840/150‑1 (MQ=60)
                                              ATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTT                                                                                                        >  SRR19973065.2327272/1‑150 (MQ=60)
                                                ACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCACTAATAATTTTCAGGAGTGATTTTA                                                                                                      >  SRR19973065.994547/1‑150 (MQ=60)
                                                ACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTA                                                                                                      >  SRR19973065.1600947/1‑150 (MQ=60)
                                                   GAAATTTAGAACACGTAGTTAAACGAGTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATT                                                                                                   <  SRR19973065.2088285/150‑1 (MQ=60)
                                                   GAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATT                                                                                                   <  SRR19973065.2095135/150‑1 (MQ=60)
                                                   GAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATT                                                                                                   <  SRR19973065.2095619/150‑1 (MQ=60)
                                                    AAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTC                                                                                                  >  SRR19973065.216347/1‑150 (MQ=60)
                                                     AATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCT                                                                                                 <  SRR19973065.69991/150‑1 (MQ=60)
                                                     AATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCT                                                                                                 <  SRR19973065.69456/150‑1 (MQ=60)
                                                           GAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTA                                                                                           >  SRR19973065.2232216/1‑150 (MQ=60)
                                                           GAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTATCATTTA                                                                                           >  SRR19973065.2233239/1‑150 (MQ=60)
                                                            AAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAG                                                                                          >  SRR19973065.1610446/1‑150 (MQ=60)
                                                            AAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAG                                                                                          >  SRR19973065.1610424/1‑150 (MQ=60)
                                                            AAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAG                                                                                          >  SRR19973065.1610528/1‑150 (MQ=60)
                                                            AAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAG                                                                                          >  SRR19973065.3201342/1‑150 (MQ=60)
                                                             AAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGC                                                                                         >  SRR19973065.437098/1‑150 (MQ=60)
                                                             AAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGC                                                                                         <  SRR19973065.1062802/150‑1 (MQ=60)
                                                                 GTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCT                                                                                     <  SRR19973065.1932111/150‑1 (MQ=60)
                                                                 GTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCT                                                                                     <  SRR19973065.1930982/150‑1 (MQ=60)
                                                                 GTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCT                                                                                     >  SRR19973065.3491286/1‑150 (MQ=60)
                                                                 GTAGTTAAACCATTGCGATCAAATATATAATCCTTCCGGCAAACATTATCATCATGATAAGGAATCACTGATTTTGAGAAAAGATAAACATTGATACATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCT                                                                                     <  SRR19973065.1932090/150‑1 (MQ=60)
                                                                   AGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGT                                                                                   >  SRR19973065.1644764/1‑150 (MQ=60)
                                                                   AGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGT                                                                                   >  SRR19973065.1644024/1‑150 (MQ=60)
                                                                   AGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGT                                                                                   >  SRR19973065.2688626/1‑150 (MQ=60)
                                                                    GTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTG                                                                                  <  SRR19973065.2566672/150‑1 (MQ=60)
                                                                        AACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTT                                                                              <  SRR19973065.2450274/150‑1 (MQ=60)
                                                                         ACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTC                                                                             <  SRR19973065.2577177/150‑1 (MQ=60)
                                                                          CGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCA                                                                            >  SRR19973065.2586180/1‑150 (MQ=60)
                                                                          CGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCA                                                                            <  SRR19973065.490679/150‑1 (MQ=60)
                                                                                     AAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATCAATGGGCTGGCAATAATAATTTTCTGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACACAAAG                                                                 >  SRR19973065.2507558/1‑150 (MQ=60)
                                                                                       ATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCC                                                               <  SRR19973065.1865485/150‑1 (MQ=60)
                                                                                       ATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCC                                                               <  SRR19973065.1865712/150‑1 (MQ=60)
                                                                                        TATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCC                                                              <  SRR19973065.1145309/150‑1 (MQ=60)
                                                                                          TTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTT                                                            >  SRR19973065.1327174/1‑150 (MQ=60)
                                                                                          TTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTT                                                            <  SRR19973065.548268/150‑1 (MQ=60)
                                                                                            TAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCACTAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAA                                                          >  SRR19973065.3292691/1‑150 (MQ=60)
                                                                                            TAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAA                                                          >  SRR19973065.3292586/1‑150 (MQ=60)
                                                                                             AATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAG                                                         <  SRR19973065.1933677/150‑1 (MQ=60)
                                                                                              ATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGT                                                        <  SRR19973065.186258/150‑1 (MQ=60)
                                                                                                CCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTT                                                      <  SRR19973065.982816/150‑1 (MQ=60)
                                                                                                 CTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTC                                                     >  SRR19973065.3445160/1‑150 (MQ=60)
                                                                                                  TTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCG                                                    <  SRR19973065.3376866/150‑1 (MQ=60)
                                                                                                     CGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGC                                                 <  SRR19973065.217632/150‑1 (MQ=60)
                                                                                                          CTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCT                                            <  SRR19973065.1950580/150‑1 (MQ=60)
                                                                                                           TCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTC                                           >  SRR19973065.2567603/1‑150 (MQ=60)
                                                                                                             ATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCAC                                         >  SRR19973065.1208442/1‑150 (MQ=60)
                                                                                                               TATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACAT                                       >  SRR19973065.1194186/1‑150 (MQ=60)
                                                                                                                ATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAACTGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATT                                      <  SRR19973065.2958335/150‑1 (MQ=60)
                                                                                                                ATCATAATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATT                                      >  SRR19973065.204908/1‑150 (MQ=60)
                                                                                                                 TCATAATTATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTT                                     <  SRR19973065.1917456/150‑1 (MQ=60)
                                                                                                                      ATGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTA                                >  SRR19973065.345178/1‑150 (MQ=60)
                                                                                                                       TGATAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTAT                               >  SRR19973065.3280052/1‑150 (MQ=60)
                                                                                                                          TAAGGAATCACTGATTTTGAGAAAAGATAAAAATTGATTAATGGGCTGGCAATTATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATT                            <  SRR19973065.2112515/150‑1 (MQ=60)
                                                                                                                            AGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTC                          >  SRR19973065.853768/1‑150 (MQ=60)
                                                                                                                            AGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTC                          >  SRR19973065.853687/1‑150 (MQ=60)
                                                                                                                            AGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTC                          >  SRR19973065.853632/1‑150 (MQ=60)
                                                                                                                            AGGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTC                          >  SRR19973065.1144886/1‑150 (MQ=60)
                                                                                                                             GGAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCC                         <  SRR19973065.3533419/150‑1 (MQ=60)
                                                                                                                              GAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCC                        <  SRR19973065.3409561/150‑1 (MQ=60)
                                                                                                                              GAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCC                        <  SRR19973065.2313913/150‑1 (MQ=60)
                                                                                                                              GAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCC                        <  SRR19973065.2137784/150‑1 (MQ=60)
                                                                                                                              GAATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCC                        <  SRR19973065.1810980/150‑1 (MQ=60)
                                                                                                                               AATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCG                       <  SRR19973065.3112970/150‑1 (MQ=60)
                                                                                                                                ATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTCAGTTTCGAGACGCCTCACATTTTTTTATATTTCCCGC                      >  SRR19973065.1396620/1‑150 (MQ=60)
                                                                                                                                ATCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGC                      >  SRR19973065.2627278/1‑150 (MQ=60)
                                                                                                                                 TCACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCC                     >  SRR19973065.1596296/1‑150 (MQ=60)
                                                                                                                                  CACTGATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCA                    >  SRR19973065.894098/1‑150 (MQ=60)
                                                                                                                                      GATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACC                >  SRR19973065.1678411/1‑150 (MQ=60)
                                                                                                                                      GATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACC                >  SRR19973065.1321/1‑150 (MQ=60)
                                                                                                                                       ATTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCT               <  SRR19973065.3182942/150‑1 (MQ=60)
                                                                                                                                        TTTTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTG              <  SRR19973065.2260128/150‑1 (MQ=60)
                                                                                                                                          TTGAGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTCAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGC            <  SRR19973065.2192898/150‑1 (MQ=60)
                                                                                                                                             AGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAAG         >  SRR19973065.2669731/1‑150 (MQ=60)
                                                                                                                                             AGAAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAAG         >  SRR19973065.662548/1‑150 (MQ=60)
                                                                                                                                               AAAAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAAGAG       <  SRR19973065.3043620/150‑1 (MQ=60)
                                                                                                                                                 AAGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAAGAGTG     <  SRR19973065.152191/150‑1 (MQ=60)
                                                                                                                                                  AGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAAGAGTGG    <  SRR19973065.1571737/150‑1 (MQ=60)
                                                                                                                                                  AGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAAGAGTGG    >  SRR19973065.1755984/1‑150 (MQ=60)
                                                                                                                                                  AGATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAAGAGTGG    >  SRR19973065.1753275/1‑150 (MQ=60)
                                                                                                                                                   GATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAAGAGTGGT   <  SRR19973065.702613/150‑1 (MQ=60)
                                                                                                                                                    ATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAAGAGTGGTG  >  SRR19973065.423736/1‑150 (MQ=60)
                                                                                                                                                    ATAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAAGAGTGGTG  <  SRR19973065.858487/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
TTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTCAGGAGTGATTTTAATTCTCATTTAGCACCTGTGAGTTCATTCACAAAAAGCCCTTAAGTTTCGAGCCGCCTCACATTTTTTTATATTTCCCGCCAAACCTGGCAAGAGTGGTG  >  CP009273/3009038‑3009335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑