Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F181 I0 R1 1262 323.1 10297870 97.5% 10040423 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,164,230 C→A 43.4% R130S (CGT→AGT)  qseC → quorum sensing sensory histidine kinase in two‑component regulatory system with QseB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,164,2300CA43.4% 296.5 / inf 341R130S (CGT→AGT) qseCquorum sensing sensory histidine kinase in two‑component regulatory system with QseB
Reads supporting (aligned to +/- strand):  ref base C (86/107);  new base A (72/76);  total (158/183)
Fisher's exact test for biased strand distribution p-value = 5.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

Only 100 of 341 total aligned reads displayed.

TGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGTTAATCATCATG  >  CP009273/3164082‑3164376
                                                                                                                                                    |                                                                                                                                                  
tGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCg                                                                                                                                                   >  1:1708390/1‑150 (MQ=255)
  ttGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGtt                                                                                                                                                 <  1:598467/150‑1 (MQ=255)
   tgatgaCGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGttt                                                                                                                                                >  1:3867252/1‑150 (MQ=255)
    gatgaCGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGtttt                                                                                                                                               <  2:1683697/150‑1 (MQ=255)
    gatgaCGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGtttt                                                                                                                                               <  1:771082/150‑1 (MQ=255)
    gatgaCGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGtttt                                                                                                                                               <  1:1350670/150‑1 (MQ=255)
     atgaCGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTg                                                                                                                                              <  1:3297373/150‑1 (MQ=255)
      tgaCGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGt                                                                                                                                             >  1:2665480/1‑150 (MQ=255)
        aCGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCt                                                                                                                                           <  2:2790316/150‑1 (MQ=255)
        aCGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCt                                                                                                                                           >  1:1275978/1‑150 (MQ=255)
         cGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTg                                                                                                                                          <  1:1402315/150‑1 (MQ=255)
          gATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTgg                                                                                                                                         >  2:3405002/1‑150 (MQ=255)
          gATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTgg                                                                                                                                         >  1:3218106/1‑150 (MQ=255)
            tGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGAt                                                                                                                                       <  1:3401981/150‑1 (MQ=255)
            tGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACGGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGAt                                                                                                                                       <  1:2205559/150‑1 (MQ=255)
               gcTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGAc                                                                                                                                    >  1:1924/1‑150 (MQ=255)
               gcTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGAc                                                                                                                                    >  1:3797085/1‑150 (MQ=255)
                cTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGAcc                                                                                                                                   <  1:483675/150‑1 (MQ=255)
                 tGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCt                                                                                                                                  <  1:1488531/150‑1 (MQ=255)
                     cTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCAcc                                                                                                                              >  2:124423/1‑150 (MQ=255)
                       ttGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTg                                                                                                                            <  1:528294/150‑1 (MQ=255)
                          ccATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATg                                                                                                                         >  1:4488527/1‑150 (MQ=255)
                             tCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCa                                                                                                                      <  2:4240688/150‑1 (MQ=255)
                                      acgacgGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCa                                                                                                             <  2:1549180/150‑1 (MQ=255)
                                       cgacgGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCAt                                                                                                            <  1:434256/150‑1 (MQ=255)
                                         acgGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCg                                                                                                          <  2:473532/150‑1 (MQ=255)
                                           ggCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCgtt                                                                                                        <  2:2535572/150‑1 (MQ=255)
                                           ggCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCgtt                                                                                                        <  1:831666/150‑1 (MQ=255)
                                           ggCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCgtt                                                                                                        <  2:35510/150‑1 (MQ=255)
                                               gAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGttgttg                                                                                                    >  1:2842979/1‑150 (MQ=255)
                                               gAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGttgttg                                                                                                    <  2:3223780/150‑1 (MQ=255)
                                                aaTGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTgg                                                                                                   >  1:4280905/1‑150 (MQ=255)
                                                 aTGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGc                                                                                                  <  2:4330630/150‑1 (MQ=255)
                                                  tGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGcc                                                                                                 >  2:4094545/1‑150 (MQ=255)
                                                  tGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGcc                                                                                                 >  1:4023358/1‑150 (MQ=255)
                                                   ggTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCa                                                                                                <  2:3200016/150‑1 (MQ=255)
                                                   ggTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCa                                                                                                >  2:900281/1‑150 (MQ=255)
                                                    gTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAg                                                                                               <  1:2030820/150‑1 (MQ=255)
                                                        ttAATGATGGCGATAACGGAGAAGATATTCCCTATCGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAAt                                                                                           <  2:760960/150‑1 (MQ=255)
                                                           atgatgGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATggg                                                                                        >  1:4516673/1‑150 (MQ=255)
                                                             gatgGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGaa                                                                                      >  1:2511142/1‑150 (MQ=255)
                                                               tgGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATa                                                                                    <  1:3120982/150‑1 (MQ=255)
                                                               tgGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATa                                                                                    >  1:4929749/1‑150 (MQ=255)
                                                                 gCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATAcc                                                                                  <  2:3875369/150‑1 (MQ=255)
                                                                   gATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGt                                                                                >  1:3656607/1‑150 (MQ=255)
                                                                   gATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGt                                                                                <  1:2280218/150‑1 (MQ=255)
                                                                     tAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGa                                                                              <  1:4217090/150‑1 (MQ=255)
                                                                      aaCGGAGAAGATAGTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGaa                                                                             <  2:1153809/150‑1 (MQ=255)
                                                                       aCGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAg                                                                            <  2:2342461/150‑1 (MQ=255)
                                                                       aCGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAg                                                                            <  2:4054727/150‑1 (MQ=255)
                                                                         ggAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGAc                                                                          >  1:3490087/1‑150 (MQ=255)
                                                                           agaagaTATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACAt                                                                        <  2:3308103/150‑1 (MQ=255)
                                                                           agaagaTATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACAt                                                                        >  1:3957709/1‑150 (MQ=255)
                                                                                 tatTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCt                                                                  >  2:2073777/1‑150 (MQ=255)
                                                                                  atTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTg                                                                 >  1:1398817/1‑150 (MQ=255)
                                                                                   ttCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTgg                                                                <  1:3922033/150‑1 (MQ=255)
                                                                                    tCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGc                                                               <  1:1534577/150‑1 (MQ=255)
                                                                                     cccTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCTTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCg                                                              >  2:583657/1‑150 (MQ=255)
                                                                                      ccTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGa                                                             >  1:3432144/1‑150 (MQ=255)
                                                                                      ccTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGa                                                             >  2:1286709/1‑150 (MQ=255)
                                                                                           aGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGAttgtt                                                        <  2:1123499/150‑1 (MQ=255)
                                                                                              tATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGcc                                                     >  1:4624374/1‑150 (MQ=255)
                                                                                                 cAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCggg                                                  >  1:74044/1‑150 (MQ=255)
                                                                                                     gggAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAAt                                              >  2:4163544/1‑150 (MQ=255)
                                                                                                     gggAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAAt                                              <  1:3080124/150‑1 (MQ=255)
                                                                                                     gggAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAAt                                              <  2:3738266/150‑1 (MQ=255)
                                                                                                      ggAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAAtt                                             >  2:1806519/1‑150 (MQ=255)
                                                                                                      ggAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAAtt                                             <  2:3797085/150‑1 (MQ=255)
                                                                                                      ggAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAAtt                                             >  1:4440084/1‑150 (MQ=255)
                                                                                                       gAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTg                                            >  1:99297/1‑150 (MQ=255)
                                                                                                         aGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGAt                                          <  2:3983131/150‑1 (MQ=255)
                                                                                                          ggTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATc                                         <  1:1241650/150‑1 (MQ=255)
                                                                                                          ggTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATc                                         <  2:2665480/150‑1 (MQ=255)
                                                                                                            ttttGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATccc                                       <  1:1808595/150‑1 (MQ=255)
                                                                                                             tttGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGGTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCg                                      >  2:5128576/1‑150 (MQ=255)
                                                                                                              ttGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGt                                     <  2:1168148/150‑1 (MQ=255)
                                                                                                               tGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTg                                    >  1:3679615/1‑150 (MQ=255)
                                                                                                                 cTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGc                                  >  2:240034/1‑150 (MQ=255)
                                                                                                                 cTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGc                                  >  2:3122091/1‑150 (MQ=255)
                                                                                                                  tGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCt                                 <  1:3102692/150‑1 (MQ=255)
                                                                                                                   gACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTg                                >  2:3025927/1‑150 (MQ=255)
                                                                                                                   gACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTg                                <  2:847534/150‑1 (MQ=255)
                                                                                                                     cGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGt                              >  2:2462573/1‑150 (MQ=255)
                                                                                                                          aaCTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCAc                         >  1:2692257/1‑150 (MQ=255)
                                                                                                                            cNGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTg                       >  1:4118056/1‑150 (MQ=255)
                                                                                                                               gTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCg                    <  1:1050197/150‑1 (MQ=255)
                                                                                                                               gTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCg                    <  1:1148763/150‑1 (MQ=255)
                                                                                                                                tCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGa                   <  1:1095327/150‑1 (MQ=255)
                                                                                                                                tCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGa                   <  2:2603749/150‑1 (MQ=255)
                                                                                                                                  ggTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGAtt                 <  2:6046/150‑1 (MQ=255)
                                                                                                                                     gAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATg              >  2:1912845/1‑150 (MQ=255)
                                                                                                                                      aaGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGt             <  2:4698921/150‑1 (MQ=255)
                                                                                                                                         aCGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGTTaa          <  1:2647187/150‑1 (MQ=255)
                                                                                                                                           gATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGTTAatc        <  1:235110/150‑1 (MQ=255)
                                                                                                                                             tCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGTTAatcat      >  2:2223171/1‑150 (MQ=255)
                                                                                                                                              ccTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGTTAatcatc     >  2:724130/1‑150 (MQ=255)
                                                                                                                                               cTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGTTAAtcatca    <  2:1479196/150‑1 (MQ=255)
                                                                                                                                               cTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGTTAAtcatca    <  2:2359134/150‑1 (MQ=255)
                                                                                                                                               cTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGTTAAtcatca    <  1:679157/150‑1 (MQ=255)
                                                                                                                                                 tGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGTTAATCATCatg  <  2:4488527/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGTTAATCATCATG  >  CP009273/3164082‑3164376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A