Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F202 I0 R1 1397 275.7 9089454 95.2% 8653160 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,164,230 C→A 67.8% R130S (CGT→AGT)  qseC → quorum sensing sensory histidine kinase in two‑component regulatory system with QseB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,164,2300CA67.8% 254.1 / 298.7 267R130S (CGT→AGT) qseCquorum sensing sensory histidine kinase in two‑component regulatory system with QseB
Reads supporting (aligned to +/- strand):  ref base C (34/52);  new base A (112/69);  total (146/121)
Fisher's exact test for biased strand distribution p-value = 9.48e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01

Only 100 of 268 total aligned reads displayed.

ATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGTTAATCATCATG  >  CP009273/3164081‑3164376
                                                                                                                                                     |                                                                                                                                                  
aTGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGc                                                                                                                                                    <  2:2775430/150‑1 (MQ=255)
  gTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGt                                                                                                                                                  >  1:1296961/1‑150 (MQ=255)
   ttGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGtt                                                                                                                                                 <  2:179311/150‑1 (MQ=255)
    tgatgaCGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGACTGGACCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCTGTGAAGACGATCATTGGAGttt                                                                                                                                                >  2:2856402/1‑150 (MQ=255)
    tgatgaCGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGttt                                                                                                                                                >  1:1525239/1‑150 (MQ=255)
      atgaCGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTg                                                                                                                                              <  1:1546977/150‑1 (MQ=255)
       tgaCGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGt                                                                                                                                             <  2:1914322/150‑1 (MQ=255)
       tgaCGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGt                                                                                                                                             >  2:513914/1‑150 (MQ=255)
       tgaCGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGt                                                                                                                                             <  1:3543861/150‑1 (MQ=255)
          cGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTg                                                                                                                                          >  1:4316649/1‑150 (MQ=255)
                gcTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGAc                                                                                                                                    >  2:1982742/1‑150 (MQ=255)
                gcTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGAc                                                                                                                                    >  2:3158505/1‑150 (MQ=255)
                 cTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGAcc                                                                                                                                   <  2:4423499/150‑1 (MQ=255)
                   gACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTc                                                                                                                                 >  1:1130024/1‑150 (MQ=255)
                   gACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTc                                                                                                                                 <  2:3669703/150‑1 (MQ=255)
                        ttGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTg                                                                                                                            <  2:1170703/150‑1 (MQ=255)
                         tGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGa                                                                                                                           <  2:1679733/150‑1 (MQ=255)
                          gCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGAt                                                                                                                          >  1:284723/1‑150 (MQ=255)
                            cATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATgg                                                                                                                        <  1:1640336/150‑1 (MQ=255)
                             aTCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGc                                                                                                                       <  2:3527299/150‑1 (MQ=255)
                                   aCCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAatat                                                                                                                 >  2:4056048/1‑150 (MQ=255)
                                            ggCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCgtt                                                                                                        >  1:3998646/1‑150 (MQ=255)
                                            ggCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCgtt                                                                                                        <  2:4325318/150‑1 (MQ=255)
                                             gCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCgttg                                                                                                       <  2:1969508/150‑1 (MQ=255)
                                              cAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCgttgt                                                                                                      >  2:4516005/1‑150 (MQ=255)
                                               aGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCgttgtt                                                                                                     <  2:3309048/150‑1 (MQ=255)
                                                gAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGttgttg                                                                                                    >  2:2241874/1‑150 (MQ=255)
                                                    ggTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCa                                                                                                >  2:683725/1‑150 (MQ=255)
                                                    ggTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGAAGGCAAATATCGCATCGTTGTTGGCCa                                                                                                >  2:2957917/1‑150 (MQ=255)
                                                    ggTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCa                                                                                                <  1:3751626/150‑1 (MQ=255)
                                                      tCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAgg                                                                                              <  2:4221770/150‑1 (MQ=255)
                                                             tgatgGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGa                                                                                       <  1:3339095/150‑1 (MQ=255)
                                                              gatgGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGaa                                                                                      >  2:162924/1‑150 (MQ=255)
                                                               atgGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAAt                                                                                     <  2:113245/150‑1 (MQ=255)
                                                                  gCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATAcc                                                                                  <  1:2323014/150‑1 (MQ=255)
                                                                  gCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATAcc                                                                                  <  1:4339255/150‑1 (MQ=255)
                                                                  gCGATAACGGAGAAGATAGTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATAcc                                                                                  <  2:917144/150‑1 (MQ=255)
                                                                    gATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGt                                                                                <  2:3215044/150‑1 (MQ=255)
                                                                     aTAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTg                                                                               >  1:2526052/1‑150 (MQ=255)
                                                                      tAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGa                                                                              <  2:2320854/150‑1 (MQ=255)
                                                                      tAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGa                                                                              <  2:17029/150‑1 (MQ=255)
                                                                      tAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGa                                                                              <  2:398577/150‑1 (MQ=255)
                                                                      tAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGa                                                                              >  1:4337607/1‑150 (MQ=255)
                                                                        aCGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCGTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAg                                                                            >  2:3703774/1‑150 (MQ=255)
                                                                          ggAGAAGATATTCCCTATATCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGATGGCCAGGAATGGGAATACCGTGAAGAc                                                                          <  2:2675259/150‑1 (MQ=255)
                                                                          ggAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGAc                                                                          >  2:4407667/1‑150 (MQ=255)
                                                                          ggAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGAc                                                                          <  2:140600/150‑1 (MQ=255)
                                                                           gatatgttatTCCCTATTGCTATCAACGTGAAGGTTTTGCTGTATGGCATCTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATTGCAAATTTCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGTCa                                                                         <  2:1106950/143‑1 (MQ=255)
                                                                           gagaAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACa                                                                         <  1:3599911/150‑1 (MQ=255)
                                                                           gagaAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACa                                                                         >  1:734396/1‑150 (MQ=255)
                                                                           gagaAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACa                                                                         >  1:3015659/1‑150 (MQ=255)
                                                                                gaTATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGgcg                                                                    >  1:2175618/1‑150 (MQ=255)
                                                                                  tatTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGACTACCGTGAAGACATGGCGCt                                                                  >  2:2211789/1‑150 (MQ=255)
                                                                                  tatTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCt                                                                  >  2:3766894/1‑150 (MQ=255)
                                                                                     tCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGc                                                               <  1:718369/150‑1 (MQ=255)
                                                                                     tCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGc                                                               >  1:3401835/1‑150 (MQ=255)
                                                                                       ccTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGa                                                             >  2:1158792/1‑150 (MQ=255)
                                                                                       ccTATAGCTATAAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCATTGGAGTGTTGTCTGGATTTCCTCACCTCATGGCAAATAGCGCATCGTTGGTGGCCAGCAATGGGACTACCGTTAAGACATGTCGATggagt                                                             >  2:2435665/1‑147 (MQ=255)
                                                                                         tataGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGAtt                                                           >  1:901454/1‑150 (MQ=255)
                                                                                         tataGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGAtt                                                           >  1:902595/1‑150 (MQ=255)
                                                                                            aGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGAttgtt                                                        <  2:3067269/150‑1 (MQ=255)
                                                                                            aGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGAttgtt                                                        <  1:3850174/150‑1 (MQ=255)
                                                                                            aGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGAttgtt                                                        <  1:3850595/150‑1 (MQ=255)
                                                                                               tATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCATTGGAGTTTTGTCAGGATGACCTCACCTGATGGCAAATATCGCATCGTTGGTGGCCAGGAATGGGAATACCGTGAAGACAAGGCGCTGGCGATGGTTGcc                                                     >  1:2074477/1‑150 (MQ=255)
                                                                                                 tCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTATTCTCTGGATGACCTCTTCTGATGGCAAATATCGCATCGTTGTTGGACAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCgg                                                   <  1:4441333/150‑1 (MQ=255)
                                                                                                   aaCGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGc                                                 >  1:569145/1‑150 (MQ=255)
                                                                                                     cGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCac                                               >  2:3155280/1‑149 (MQ=255)
                                                                                                      gggAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATACTTGACGGTCaca                                              >  1:2674198/1‑148 (MQ=255)
                                                                                                       ggAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAAtt                                             >  1:367216/1‑150 (MQ=255)
                                                                                                       ggAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAAtt                                             <  1:4516005/150‑1 (MQ=255)
                                                                                                         aaGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGa                                           >  2:3016366/1‑150 (MQ=255)
                                                                                                          aGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGAt                                          <  1:1392496/150‑1 (MQ=255)
                                                                                                           ggTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATc                                         <  2:2424732/150‑1 (MQ=255)
                                                                                                           ggTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATc                                         >  2:2890182/1‑150 (MQ=255)
                                                                                                             ttttGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATccc                                       <  1:1422539/150‑1 (MQ=255)
                                                                                                             ttttGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATccc                                       <  2:3351913/150‑1 (MQ=255)
                                                                                                             ttttGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATccc                                       <  2:4097537/150‑1 (MQ=255)
                                                                                                               ttGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGt                                     >  1:312060/1‑150 (MQ=255)
                                                                                                                tGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTg                                    <  2:759820/150‑1 (MQ=255)
                                                                                                                  cTGACGTGCAACTGGTCGGATAAGAAGATCCTTGGCGTTTTGTCTGTATTACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGTAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGTCAATTGATACCGTgtc                                  <  2:2023316/150‑3 (MQ=255)
                                                                                                                  cTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGc                                  <  2:2022790/150‑1 (MQ=255)
                                                                                                                   tGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTGGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCt                                 <  2:2873976/150‑1 (MQ=255)
                                                                                                                    gACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTg                                >  1:2267228/1‑150 (MQ=255)
                                                                                                                          cAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCa                          >  1:3623149/1‑150 (MQ=255)
                                                                                                                           aaCTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCAc                         <  1:4391683/150‑1 (MQ=255)
                                                                                                                           aaCTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCAc                         >  1:3853283/1‑150 (MQ=255)
                                                                                                                            aCTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACt                        <  1:822210/150‑1 (MQ=255)
                                                                                                                            aCTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACt                        <  2:2479133/150‑1 (MQ=255)
                                                                                                                             cTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTg                       <  2:4118270/150‑1 (MQ=255)
                                                                                                                             cTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTg                       <  2:2576654/150‑1 (MQ=255)
                                                                                                                               ggTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGcc                     <  2:1525239/150‑1 (MQ=255)
                                                                                                                               ggTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGcc                     <  2:4155192/150‑1 (MQ=255)
                                                                                                                                    gTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTa                >  1:4080863/1‑150 (MQ=255)
                                                                                                                                    gTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTa                >  1:1915864/1‑150 (MQ=255)
                                                                                                                                        aGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGtt            >  2:740095/1‑150 (MQ=255)
                                                                                                                                         gACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGTTa           <  1:1764071/150‑1 (MQ=255)
                                                                                                                                           cGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGTTAat         <  1:1001355/150‑1 (MQ=255)
                                                                                                                                               ccTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGTTAatcatc     >  1:4232653/1‑150 (MQ=255)
                                                                                                                                                  tGGCGTTTTGTCTGGGTGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGTTAATCATCatg  >  1:2662343/1‑150 (MQ=255)
                                                                                                                                                  tGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGTTAATCATCatg  >  1:3177713/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
ATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGTTAATCATCATG  >  CP009273/3164081‑3164376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGTTAATCATCATGAT  >  CP009273/3164081‑3164378
                                                                                                                                                     |                                                                                                                                                    
ATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGA                                                                                                                                                      >  SRR19973076.140847/1‑150 (MQ=60)
  GTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGT                                                                                                                                                    >  SRR19973076.1298873/1‑150 (MQ=60)
  GTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGT                                                                                                                                                    >  SRR19973076.1298962/1‑150 (MQ=60)
    TGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTT                                                                                                                                                  >  SRR19973076.1527475/1‑150 (MQ=60)
      ATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTG                                                                                                                                                <  SRR19973076.1549245/150‑1 (MQ=60)
      ATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTG                                                                                                                                                <  SRR19973076.1549213/150‑1 (MQ=60)
      ATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTG                                                                                                                                                <  SRR19973076.1548472/150‑1 (MQ=60)
       TGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGT                                                                                                                                               <  SRR19973076.1276003/150‑1 (MQ=60)
       TGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGT                                                                                                                                               <  SRR19973076.3549011/150‑1 (MQ=60)
          CGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTG                                                                                                                                            >  SRR19973076.4323000/1‑150 (MQ=60)
          CGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTG                                                                                                                                            >  SRR19973076.4323019/1‑150 (MQ=60)
          CGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTG                                                                                                                                            >  SRR19973076.4323024/1‑150 (MQ=60)
          CGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTG                                                                                                                                            >  SRR19973076.4323044/1‑150 (MQ=60)
                   GACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTC                                                                                                                                   >  SRR19973076.1131689/1‑150 (MQ=60)
                          GCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGAT                                                                                                                            >  SRR19973076.285188/1‑150 (MQ=60)
                          GCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGAT                                                                                                                            >  SRR19973076.285165/1‑150 (MQ=60)
                          GCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGAT                                                                                                                            >  SRR19973076.285183/1‑150 (MQ=60)
                            CATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGG                                                                                                                          <  SRR19973076.1642742/150‑1 (MQ=60)
                                 TTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAAT                                                                                                                     <  SRR19973076.2842792/150‑1 (MQ=60)
                                 TTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAAT                                                                                                                     <  SRR19973076.1550196/150‑1 (MQ=60)
                                        CGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCAT                                                                                                              >  SRR19973076.1448926/1‑150 (MQ=60)
                                           CGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGT                                                                                                           >  SRR19973076.3615032/1‑150 (MQ=60)
                                            GGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTT                                                                                                          >  SRR19973076.4395487/1‑150 (MQ=60)
                                            GGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTT                                                                                                          >  SRR19973076.4395665/1‑150 (MQ=60)
                                            GGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTT                                                                                                          >  SRR19973076.4004544/1‑150 (MQ=60)
                                               AGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTT                                                                                                       >  SRR19973076.1905564/1‑150 (MQ=60)
                                                GAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTG                                                                                                      >  SRR19973076.2229869/1‑150 (MQ=60)
                                                 AATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGG                                                                                                     >  SRR19973076.4167954/1‑150 (MQ=60)
                                                 AATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGG                                                                                                     >  SRR19973076.4167938/1‑150 (MQ=60)
                                                    GGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCA                                                                                                  <  SRR19973076.3757121/150‑1 (MQ=60)
                                                       CCTTAATGATGGCTATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGA                                                                                               >  SRR19973076.3143897/1‑150 (MQ=60)
                                                       CCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCTACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGA                                                                                               <  SRR19973076.3706612/150‑1 (MQ=60)
                                                           AATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGG                                                                                           >  SRR19973076.2580404/1‑150 (MQ=60)
                                                             TGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGA                                                                                         <  SRR19973076.3343932/150‑1 (MQ=60)
                                                               ATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAAT                                                                                       >  SRR19973076.2418044/1‑150 (MQ=60)
                                                               ATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAAT                                                                                       <  SRR19973076.3432069/150‑1 (MQ=60)
                                                                  GCGATTACGGAGTAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGTTGACCTCAGCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACC                                                                                    <  SRR19973076.4345589/150‑1 (MQ=60)
                                                                  GCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACC                                                                                    <  SRR19973076.17449/150‑1 (MQ=60)
                                                                  GCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACC                                                                                    <  SRR19973076.2326350/150‑1 (MQ=60)
                                                                  GCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACC                                                                                    <  SRR19973076.4345653/150‑1 (MQ=60)
                                                                  GCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACC                                                                                    <  SRR19973076.4345663/150‑1 (MQ=60)
                                                                  GCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACC                                                                                    <  SRR19973076.1167851/150‑1 (MQ=60)
                                                                     ATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTG                                                                                 >  SRR19973076.2529727/1‑150 (MQ=60)
                                                                     ATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTG                                                                                 >  SRR19973076.2531421/1‑150 (MQ=60)
                                                                      TAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGA                                                                                >  SRR19973076.4344013/1‑150 (MQ=60)
                                                                      TAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGA                                                                                >  SRR19973076.1968464/1‑150 (MQ=60)
                                                                           GAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACA                                                                           <  SRR19973076.3604977/150‑1 (MQ=60)
                                                                           GAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACA                                                                           <  SRR19973076.3604976/150‑1 (MQ=60)
                                                                           GAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACA                                                                           <  SRR19973076.3605143/150‑1 (MQ=60)
                                                                           GAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACA                                                                           >  SRR19973076.735489/1‑150 (MQ=60)
                                                                           GAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACA                                                                           >  SRR19973076.735488/1‑150 (MQ=60)
                                                                           GAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACA                                                                           >  SRR19973076.3020048/1‑150 (MQ=60)
                                                                            AGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACAT                                                                          >  SRR19973076.376389/1‑150 (MQ=60)
                                                                               AGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGC                                                                       <  SRR19973076.633277/150‑1 (MQ=60)
                                                                                GATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCG                                                                      >  SRR19973076.2178759/1‑150 (MQ=60)
                                                                                GATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCG                                                                      >  SRR19973076.2178540/1‑150 (MQ=60)
                                                                                  TATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCT                                                                    >  SRR19973076.2175120/1‑150 (MQ=60)
                                                                                     TCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGC                                                                 <  SRR19973076.719441/150‑1 (MQ=60)
                                                                                     TCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGC                                                                 >  SRR19973076.7228/1‑150 (MQ=60)
                                                                                     TCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGC                                                                 >  SRR19973076.3406595/1‑150 (MQ=60)
                                                                                     TCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGC                                                                 >  SRR19973076.3406763/1‑150 (MQ=60)
                                                                                     TCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGC                                                                 >  SRR19973076.7609/1‑150 (MQ=60)
                                                                                         TATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATT                                                             >  SRR19973076.902811/1‑150 (MQ=60)
                                                                                         TATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATT                                                             >  SRR19973076.902792/1‑150 (MQ=60)
                                                                                         TATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATT                                                             >  SRR19973076.903933/1‑150 (MQ=60)
                                                                                         TATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCACATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATT                                                             >  SRR19973076.903931/1‑150 (MQ=60)
                                                                                         TATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATT                                                             >  SRR19973076.4494419/1‑150 (MQ=60)
                                                                                            AGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTT                                                          <  SRR19973076.3856297/150‑1 (MQ=60)
                                                                                            AGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTT                                                          <  SRR19973076.3856265/150‑1 (MQ=60)
                                                                                            AGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTT                                                          <  SRR19973076.3856267/150‑1 (MQ=60)
                                                                                            AGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTT                                                          <  SRR19973076.3855694/150‑1 (MQ=60)
                                                                                            AGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTT                                                          <  SRR19973076.3855846/150‑1 (MQ=60)
                                                                                            AGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTT                                                          <  SRR19973076.3856300/150‑1 (MQ=60)
                                                                                               TATCACCGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCC                                                       <  SRR19973076.3455607/150‑1 (MQ=60)
                                                                                               TATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCATTGGAGTTTTGTCAGGATGACCTCACCTGATGGCAAATATCGCATCGTTGGTGGCCAGGAATGGGAATACCGTGAAGACAAGGCGCTGGCGATGGTTGCC                                                       >  SRR19973076.2077498/1‑150 (MQ=60)
                                                                                                 TCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGCCCACTGCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGG                                                     <  SRR19973076.4447854/150‑1 (MQ=60)
                                                                                                 TCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGG                                                     <  SRR19973076.4450640/150‑1 (MQ=60)
                                                                                                 TCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTATTCTCTGGATGACCTCTTCTGATGGCAAATATCGCATCGTTGTTGGACAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGG                                                     <  SRR19973076.4447944/150‑1 (MQ=60)
                                                                                                  CAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGG                                                    <  SRR19973076.3615329/150‑1 (MQ=60)
                                                                                                   AACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGC                                                   >  SRR19973076.1305141/1‑150 (MQ=60)
                                                                                                   AACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGC                                                   >  SRR19973076.2860557/1‑150 (MQ=60)
                                                                                                   AACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGC                                                   >  SRR19973076.570034/1‑150 (MQ=60)
                                                                                                     CGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAA                                                 <  SRR19973076.2245097/150‑1 (MQ=60)
                                                                                                      GGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATacttgacggtcaca                                                >  SRR19973076.2678080/1‑136 (MQ=60)
                                                                                                       GGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATT                                               >  SRR19973076.367793/1‑150 (MQ=60)
                                                                                                       GGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATT                                               <  SRR19973076.4522702/150‑1 (MQ=60)
                                                                                                          AGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGAT                                            <  SRR19973076.1394553/150‑1 (MQ=60)
                                                                                                          AGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGAT                                            >  SRR19973076.900566/1‑150 (MQ=60)
                                                                                                           GGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATC                                           >  SRR19973076.3175106/1‑150 (MQ=60)
                                                                                                             TTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCC                                         <  SRR19973076.3717943/150‑1 (MQ=60)
                                                                                                             TTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCC                                         <  SRR19973076.1424630/150‑1 (MQ=60)
                                                                                                             TTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCC                                         <  SRR19973076.1423425/150‑1 (MQ=60)
                                                                                                               TTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGT                                       >  SRR19973076.312559/1‑150 (MQ=60)
                                                                                                                 GCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGG                                     >  SRR19973076.3336505/1‑150 (MQ=60)
                                                                                                                 GCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGG                                     >  SRR19973076.3336398/1‑150 (MQ=60)
                                                                                                                   TGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCT                                   >  SRR19973076.2671149/1‑150 (MQ=60)
                                                                                                                    GACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTG                                  >  SRR19973076.2270478/1‑150 (MQ=60)
                                                                                                                          CAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCA                            >  SRR19973076.3628419/1‑150 (MQ=60)
                                                                                                                          CAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCA                            >  SRR19973076.3628754/1‑150 (MQ=60)
                                                                                                                          CAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCA                            <  SRR19973076.3047152/150‑1 (MQ=60)
                                                                                                                           AACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCAC                           >  SRR19973076.4546322/1‑150 (MQ=60)
                                                                                                                           AACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCAC                           >  SRR19973076.4546339/1‑150 (MQ=60)
                                                                                                                           AACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCAC                           >  SRR19973076.3859050/1‑150 (MQ=60)
                                                                                                                           AACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCAC                           <  SRR19973076.4398163/150‑1 (MQ=60)
                                                                                                                           AACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCAC                           >  SRR19973076.1669462/1‑150 (MQ=60)
                                                                                                                           AACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCAC                           >  SRR19973076.3859110/1‑150 (MQ=60)
                                                                                                                           AACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCAC                           >  SRR19973076.3858963/1‑150 (MQ=60)
                                                                                                                           AACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCAC                           >  SRR19973076.2305314/1‑150 (MQ=60)
                                                                                                                            ACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACT                          <  SRR19973076.823421/150‑1 (MQ=60)
                                                                                                                                    GTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTA                  >  SRR19973076.1918609/1‑150 (MQ=60)
                                                                                                                                    GTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTA                  >  SRR19973076.1918632/1‑150 (MQ=60)
                                                                                                                                    GTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTA                  >  SRR19973076.4086887/1‑150 (MQ=60)
                                                                                                                                    GTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTA                  >  SRR19973076.4086859/1‑150 (MQ=60)
                                                                                                                                     TGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTAT                 >  SRR19973076.1036369/1‑150 (MQ=60)
                                                                                                                                         GACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGTTA             <  SRR19973076.1766623/150‑1 (MQ=60)
                                                                                                                                           CGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGTTAAT           <  SRR19973076.1002841/150‑1 (MQ=60)
                                                                                                                                            GATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGTTAATC          >  SRR19973076.2348199/1‑150 (MQ=60)
                                                                                                                                            GATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGTTAATC          >  SRR19973076.1524143/1‑150 (MQ=60)
                                                                                                                                               CCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGTTAATCATC       >  SRR19973076.726819/1‑150 (MQ=60)
                                                                                                                                               CCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGTTAATCATC       >  SRR19973076.4238905/1‑150 (MQ=60)
                                                                                                                                                  TGGCGTTTTGTCTGGGTGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGTTAATCATCATG    >  SRR19973076.2666212/1‑150 (MQ=60)
                                                                                                                                                  TGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGTTAATCATCATG    >  SRR19973076.3182317/1‑150 (MQ=60)
                                                                                                                                                    GAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGTTAATCATCATGAT  >  SRR19973076.984931/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
ATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGTTAATCATCATGAT  >  CP009273/3164081‑3164378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑