Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F142 I0 R1 859 274.6 8723680 96.4% 8409627 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,164,230 C→A 44.7% R130S (CGT→AGT)  qseC → quorum sensing sensory histidine kinase in two‑component regulatory system with QseB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,164,2300CA44.7% 169.4 / inf 244R130S (CGT→AGT) qseCquorum sensing sensory histidine kinase in two‑component regulatory system with QseB
Reads supporting (aligned to +/- strand):  ref base C (65/70);  new base A (50/59);  total (115/129)
Fisher's exact test for biased strand distribution p-value = 7.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

Only 100 of 244 total aligned reads displayed.

GTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGTTAATCATCATG  >  CP009273/3164083‑3164376
                                                                                                                                                   |                                                                                                                                                  
gTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGt                                                                                                                                                  >  1:3174858/1‑150 (MQ=255)
   gatgaCGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGtttt                                                                                                                                               <  2:3093476/150‑1 (MQ=255)
      gaCGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTc                                                                                                                                            <  1:3135953/150‑1 (MQ=255)
       aCGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCt                                                                                                                                           <  2:1707602/150‑1 (MQ=255)
        cGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTg                                                                                                                                          <  1:1304855/150‑1 (MQ=255)
          aTGCGCCGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGa                                                                                                                                        >  1:1670452/1‑150 (MQ=255)
           tGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGAt                                                                                                                                       >  2:2153655/1‑150 (MQ=255)
              gcTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGAc                                                                                                                                    <  1:4941/150‑1 (MQ=255)
               cTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGAcc                                                                                                                                   <  1:3650468/150‑1 (MQ=255)
                tGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCt                                                                                                                                  <  2:2843930/150‑1 (MQ=255)
                 gACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTc                                                                                                                                 <  1:731101/150‑1 (MQ=255)
                  aCCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCa                                                                                                                                <  1:1406405/150‑1 (MQ=255)
                  aCCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCa                                                                                                                                <  1:1100640/150‑1 (MQ=255)
                     tttGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCt                                                                                                                             <  1:1129044/150‑1 (MQ=255)
                       tGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGa                                                                                                                           <  1:3995443/150‑1 (MQ=255)
                       tGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGa                                                                                                                           <  1:1919597/150‑1 (MQ=255)
                         ccATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATg                                                                                                                         >  2:2857819/1‑150 (MQ=255)
                                tACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAata                                                                                                                  >  2:2449089/1‑150 (MQ=255)
                                  cccACGACGGCAGACTGGTCCTTAATGATGGCGATAACGGAGAAGATAGTCCCTAGAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATc                                                                                                                <  1:2557012/150‑1 (MQ=255)
                                   ccACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCg                                                                                                               <  2:1005836/150‑1 (MQ=255)
                                   ccACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCg                                                                                                               >  2:2720649/1‑150 (MQ=255)
                                    cACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGc                                                                                                              >  1:3077457/1‑150 (MQ=255)
                                      cgacgGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCAt                                                                                                            <  2:1566717/150‑1 (MQ=255)
                                       gacgGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATc                                                                                                           <  2:3324369/150‑1 (MQ=255)
                                        acgGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCg                                                                                                          <  1:2582115/150‑1 (MQ=255)
                                         cgGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCgt                                                                                                         >  2:773701/1‑150 (MQ=255)
                                          ggCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCgtt                                                                                                        <  1:4279373/150‑1 (MQ=255)
                                          ggCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCgtt                                                                                                        <  1:1552747/150‑1 (MQ=255)
                                             aGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCgttgtt                                                                                                     <  2:3599441/150‑1 (MQ=255)
                                             aGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCgttgtt                                                                                                     >  2:3461794/1‑150 (MQ=255)
                                             aGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCgttgtt                                                                                                     <  1:2806216/150‑1 (MQ=255)
                                                aTGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGc                                                                                                  <  2:3789034/150‑1 (MQ=255)
                                                 tGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGcc                                                                                                 >  2:604147/1‑150 (MQ=255)
                                                  ggTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCa                                                                                                <  2:14380/150‑1 (MQ=255)
                                                   gTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAg                                                                                               >  1:402209/1‑150 (MQ=255)
                                                   gTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAg                                                                                               <  2:2373203/150‑1 (MQ=255)
                                                    tCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAgg                                                                                              >  1:1801677/1‑150 (MQ=255)
                                                      cTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGaa                                                                                            <  2:3983259/150‑1 (MQ=255)
                                                      cTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGaa                                                                                            >  2:2946715/1‑150 (MQ=255)
                                                          atgatgGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATggg                                                                                        >  2:3555361/1‑150 (MQ=255)
                                                           tgatgGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGa                                                                                       >  2:2226668/1‑150 (MQ=255)
                                                           tgatgGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGa                                                                                       <  1:4164829/150‑1 (MQ=255)
                                                           tgatgGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGa                                                                                       <  1:1979211/150‑1 (MQ=255)
                                                             atgGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAAt                                                                                     <  1:223501/150‑1 (MQ=255)
                                                              tgGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATa                                                                                    >  2:2661638/1‑150 (MQ=255)
                                                                  gATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGt                                                                                >  2:957602/1‑150 (MQ=255)
                                                                  gATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGt                                                                                >  1:38764/1‑150 (MQ=255)
                                                                       cGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGa                                                                           >  1:4356510/1‑150 (MQ=255)
                                                                          agaagaTATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACAt                                                                        <  2:2383250/150‑1 (MQ=255)
                                                                          agaagaTATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACAt                                                                        >  2:1459002/1‑150 (MQ=255)
                                                                           gaagaTATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATg                                                                       >  2:1306096/1‑150 (MQ=255)
                                                                              gaTATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGgcg                                                                    <  2:37358/150‑1 (MQ=255)
                                                                              gaTAGTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGgcg                                                                    <  2:2212855/150‑1 (MQ=255)
                                                                                      cTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGAt                                                            <  2:35285/150‑1 (MQ=255)
                                                                                      cTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGAt                                                            <  2:2000458/150‑1 (MQ=255)
                                                                                         taGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGAttgt                                                         <  1:13623/150‑1 (MQ=255)
                                                                                         taGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGAttgt                                                         <  2:4317330/150‑1 (MQ=255)
                                                                                          aGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGAttgtt                                                        <  2:2653369/150‑1 (MQ=255)
                                                                                          aGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGAttgtt                                                        >  1:1870516/1‑150 (MQ=255)
                                                                                           gCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGAttgttg                                                       <  1:2584223/150‑1 (MQ=255)
                                                                                              aTCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCg                                                    >  2:3822363/1‑150 (MQ=255)
                                                                                              aTCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCg                                                    >  1:3330451/1‑150 (MQ=255)
                                                                                               tCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCgg                                                   >  2:3244669/1‑150 (MQ=255)
                                                                                                cAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCggg                                                  >  2:2846109/1‑150 (MQ=255)
                                                                                                cAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCggg                                                  >  2:2929097/1‑150 (MQ=255)
                                                                                                 aaCGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGc                                                 >  1:921882/1‑150 (MQ=255)
                                                                                                    gggAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAAt                                              >  2:2382063/1‑150 (MQ=255)
                                                                                                     ggAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAAtt                                             <  2:2586296/150‑1 (MQ=255)
                                                                                                     ggAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAAtt                                             >  1:2921267/1‑150 (MQ=255)
                                                                                                     ggAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCAACTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAAtt                                             <  1:214028/150‑1 (MQ=255)
                                                                                                      gAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTg                                            >  1:347012/1‑150 (MQ=255)
                                                                                                      gAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTg                                            >  1:2520541/1‑150 (MQ=255)
                                                                                                          gTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATcc                                        <  1:2197878/150‑1 (MQ=255)
                                                                                                          gTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATcc                                        >  2:2304074/1‑150 (MQ=255)
                                                                                                           ttttGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATccc                                       <  1:2449089/150‑1 (MQ=255)
                                                                                                              tGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTg                                    <  1:1629135/150‑1 (MQ=255)
                                                                                                                cTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGc                                  <  2:3980065/150‑1 (MQ=255)
                                                                                                                cTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGc                                  <  2:408697/150‑1 (MQ=255)
                                                                                                                   aCGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTgg                               <  2:162589/150‑1 (MQ=255)
                                                                                                                          aCTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACt                        <  2:2836507/150‑1 (MQ=255)
                                                                                                                          aCTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACt                        <  2:4205276/150‑1 (MQ=255)
                                                                                                                          aCTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACt                        <  2:589194/150‑1 (MQ=255)
                                                                                                                           cTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTg                       <  2:1381391/150‑1 (MQ=255)
                                                                                                                            tGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGc                      >  2:2130325/1‑150 (MQ=255)
                                                                                                                             ggTCGGTGAAGACGATCCTTGGCATTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGcc                     >  2:2166107/1‑150 (MQ=255)
                                                                                                                                 ggTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGAtt                 >  1:3434519/1‑150 (MQ=255)
                                                                                                                                 ggTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGAtt                 >  1:3836869/1‑150 (MQ=255)
                                                                                                                                  gTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTa                <  2:1635385/150‑1 (MQ=255)
                                                                                                                                   tGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTAt               <  1:2350488/150‑1 (MQ=255)
                                                                                                                                   tGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTAt               <  1:3568535/150‑1 (MQ=255)
                                                                                                                                    gAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGAGTATg              >  2:3691747/1‑150 (MQ=255)
                                                                                                                                        aCGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGTTaa          <  1:2917132/150‑1 (MQ=255)
                                                                                                                                         cGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTTCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGTTAat         >  1:407259/1‑150 (MQ=255)
                                                                                                                                         cGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGTTAat         <  1:1173357/150‑1 (MQ=255)
                                                                                                                                         cGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGTTAat         <  1:1005714/150‑1 (MQ=255)
                                                                                                                                         cGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGTTAat         <  1:3368449/150‑1 (MQ=255)
                                                                                                                                         cGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGTTAat         >  2:2990081/1‑150 (MQ=255)
                                                                                                                                             ccTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGTTAatcatc     <  2:1295273/150‑1 (MQ=255)
                                                                                                                                              cTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGTTAAtcatca    <  2:1031159/150‑1 (MQ=255)
                                                                                                                                                tGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGTTAATCATCatg  >  2:1952599/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGTTAATCATCATG  >  CP009273/3164083‑3164376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A