Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,164,230 C→A 100% R130S (CGT→AGT)  qseC → quorum sensing sensory histidine kinase in two‑component regulatory system with QseB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,164,2300CA99.5% 611.2 / ‑4.0 185R130S (CGT→AGT) qseCquorum sensing sensory histidine kinase in two‑component regulatory system with QseB
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (85/99);  total (86/99)
Fisher's exact test for biased strand distribution p-value = 4.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 185 total aligned reads displayed.

TGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGTTAATCATCA  >  CP009273/3164085‑3164374
                                                                                                                                                 |                                                                                                                                                
 gatgaCGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGtt                                                                                                                                               <  1:3271349/148‑1 (MQ=255)
  atgtCGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGAGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGTAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGttt                                                                                                                                              <  1:1756000/148‑1 (MQ=255)
    gaCGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTg                                                                                                                                            >  2:328632/1‑148 (MQ=255)
     aCGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGt                                                                                                                                           <  2:852146/148‑1 (MQ=255)
     aCGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGt                                                                                                                                           <  2:127453/148‑1 (MQ=255)
         tGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTgg                                                                                                                                       >  1:918873/1‑148 (MQ=255)
          gcgcTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGa                                                                                                                                      <  1:166636/148‑1 (MQ=255)
           cgcTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGAt                                                                                                                                     <  1:2202917/148‑1 (MQ=255)
            tgtGACCTTTGCGATCTTTACCCTCGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATTTTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCATCTGGTCGGTGAAGACGATCCTTGGAGTTTTGTGTGGATg                                                                                                                                    <  2:1474398/146‑1 (MQ=255)
              tGACCTGTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGAc                                                                                                                                  <  2:1398209/148‑1 (MQ=255)
               gACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGAcc                                                                                                                                 <  1:481033/148‑1 (MQ=255)
                aCCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCt                                                                                                                                >  1:3684499/1‑148 (MQ=255)
                aCCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCt                                                                                                                                >  2:206073/1‑148 (MQ=255)
                aCCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCt                                                                                                                                <  1:2996444/148‑1 (MQ=255)
                 ccTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTc                                                                                                                               <  2:545793/148‑1 (MQ=255)
                 ccTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTc                                                                                                                               <  2:546613/148‑1 (MQ=255)
                   tttGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCAc                                                                                                                             >  1:659299/1‑148 (MQ=255)
                        cATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGAt                                                                                                                        >  2:598582/1‑148 (MQ=255)
                        cATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGAt                                                                                                                        <  2:752018/148‑1 (MQ=255)
                        cATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGAt                                                                                                                        <  1:1371229/148‑1 (MQ=255)
                           cTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGc                                                                                                                     >  2:1103634/1‑148 (MQ=255)
                           cTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGc                                                                                                                     >  2:2516624/1‑148 (MQ=255)
                           cTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGc                                                                                                                     <  2:3814455/148‑1 (MQ=255)
                             ttACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCaa                                                                                                                   <  1:1999656/148‑1 (MQ=255)
                               aCCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAat                                                                                                                 <  1:1412652/148‑1 (MQ=255)
                                 ccACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAatat                                                                                                               >  2:20410/1‑148 (MQ=255)
                                 ccACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAatat                                                                                                               >  1:2616143/1‑148 (MQ=255)
                                  cACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCaaa                                                                                                                  <  1:621747/144‑1 (MQ=255)
                                  cACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCaaa                                                                                                                  >  2:3088805/1‑144 (MQ=255)
                                  cACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATc                                                                                                              >  1:1726819/1‑148 (MQ=255)
                                     gacgGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCa                                                                                                           <  2:334860/148‑1 (MQ=255)
                                     gacgGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCa                                                                                                           <  2:334879/148‑1 (MQ=255)
                                        ggCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCg                                                                                                        <  1:2663204/148‑1 (MQ=255)
                                        ggCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCg                                                                                                        <  1:2662541/148‑1 (MQ=255)
                                        ggCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCg                                                                                                        <  1:1021470/148‑1 (MQ=255)
                                         gCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCgt                                                                                                       <  1:1034358/148‑1 (MQ=255)
                                          cAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCgtt                                                                                                      >  1:3403059/1‑148 (MQ=255)
                                           aGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCgttg                                                                                                     <  2:38122/148‑1 (MQ=255)
                                             aaTGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCgttgtt                                                                                                   >  2:3675381/1‑148 (MQ=255)
                                                 gTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGcc                                                                                               <  1:1585782/148‑1 (MQ=255)
                                                 gTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGcc                                                                                               >  1:2771804/1‑148 (MQ=255)
                                                     ttAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCTCATCGTTGTTGGCCAGGa                                                                                           >  2:3450751/1‑148 (MQ=255)
                                                      tAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGac                                                                                          >  1:3436799/1‑147 (MQ=255)
                                                         tgatgGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATgg                                                                                       <  1:2268986/148‑1 (MQ=255)
                                                         tgatgGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATgg                                                                                       >  2:3801302/1‑148 (MQ=255)
                                                             ggCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAAt                                                                                   >  1:3803454/1‑148 (MQ=255)
                                                              gCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATa                                                                                  <  1:414512/148‑1 (MQ=255)
                                                                 aTAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCg                                                                               >  1:823274/1‑148 (MQ=255)
                                                                   aaCGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTg                                                                             >  2:2349374/1‑148 (MQ=255)
                                                                   aaCGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTg                                                                             >  2:1674177/1‑148 (MQ=255)
                                                                   aaCGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTg                                                                             >  2:427687/1‑148 (MQ=255)
                                                                    aCGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGa                                                                            <  1:1131289/148‑1 (MQ=255)
                                                                        agaagaTATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGAc                                                                        <  2:2116665/148‑1 (MQ=255)
                                                                         gaagaTATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACa                                                                       <  2:2825729/148‑1 (MQ=255)
                                                                         gaagaTATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACa                                                                       <  2:2545606/148‑1 (MQ=255)
                                                                          aagaTATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACAt                                                                      <  1:377191/148‑1 (MQ=255)
                                                                            gaTATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATgg                                                                    >  2:749860/1‑148 (MQ=255)
                                                                            gaTATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATgg                                                                    <  2:195275/148‑1 (MQ=255)
                                                                            gaTATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATgg                                                                    >  1:238170/1‑148 (MQ=255)
                                                                            gaTATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATgg                                                                    <  2:2125399/148‑1 (MQ=255)
                                                                             atatTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGgc                                                                   >  1:2334472/1‑148 (MQ=255)
                                                                               atTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGgcgc                                                                 >  1:1907709/1‑148 (MQ=255)
                                                                               atTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGgcgc                                                                 >  2:3692006/1‑148 (MQ=255)
                                                                                    cTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCg                                                            >  1:2499704/1‑148 (MQ=255)
                                                                                     tataGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGa                                                           <  1:2053023/148‑1 (MQ=255)
                                                                                       taGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGAtt                                                         >  2:2594895/1‑148 (MQ=255)
                                                                                        aGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGAttg                                                        >  1:658012/1‑148 (MQ=255)
                                                                                         gCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGAttgt                                                       >  2:2250167/1‑148 (MQ=255)
                                                                                         gCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGAttgt                                                       >  2:3719499/1‑148 (MQ=255)
                                                                                            aTCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGc                                                    >  2:626438/1‑148 (MQ=255)
                                                                                             tCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGcc                                                   >  1:223806/1‑148 (MQ=255)
                                                                                             tCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGcc                                                   <  2:640962/148‑1 (MQ=255)
                                                                                                 cGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGc                                               >  2:2957918/1‑148 (MQ=255)
                                                                                                   ggAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCaa                                             >  1:665581/1‑148 (MQ=255)
                                                                                                    gAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAAt                                            <  1:211055/148‑1 (MQ=255)
                                                                                                     aaGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAAtt                                           <  2:2705004/148‑1 (MQ=255)
                                                                                                     aaGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAAtt                                           <  1:2682221/148‑1 (MQ=255)
                                                                                                       ggTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGa                                         <  2:1524928/148‑1 (MQ=255)
                                                                                                       ggTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGa                                         <  1:3714013/148‑1 (MQ=255)
                                                                                                       ggTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGa                                         <  2:1489471/148‑1 (MQ=255)
                                                                                                       ggTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGa                                         >  1:2029577/1‑148 (MQ=255)
                                                                                                               tGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTgg                                 <  2:1531159/148‑1 (MQ=255)
                                                                                                                gACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGc                                <  1:612240/148‑1 (MQ=255)
                                                                                                                gACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGc                                <  1:20410/148‑1 (MQ=255)
                                                                                                                    ggCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGt                            <  2:823274/148‑1 (MQ=255)
                                                                                                                        aCTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCa                        <  2:783444/148‑1 (MQ=255)
                                                                                                                         cTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCAc                       >  1:2314635/1‑148 (MQ=255)
                                                                                                                         cTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCAc                       <  1:750260/148‑1 (MQ=255)
                                                                                                                         cTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCAc                       >  1:3590821/1‑148 (MQ=255)
                                                                                                                           ggTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTg                     <  1:1613977/148‑1 (MQ=255)
                                                                                                                              cGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCg                  <  1:2456393/148‑1 (MQ=255)
                                                                                                                              cGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCg                  <  1:2456058/148‑1 (MQ=255)
                                                                                                                                gTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGAt                >  1:3402127/1‑148 (MQ=255)
                                                                                                                                 tGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGAtt               >  1:3312905/1‑148 (MQ=255)
                                                                                                                                  gAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTa              <  1:3675381/148‑1 (MQ=255)
                                                                                                                                    aGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATg            >  2:1530841/1‑148 (MQ=255)
                                                                                                                                         aTCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGTTAat       >  1:904183/1‑148 (MQ=255)
                                                                                                                                         aTCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGTTAat       >  2:3178654/1‑148 (MQ=255)
                                                                                                                                         aTCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGTTAat       <  2:284164/148‑1 (MQ=255)
                                                                                                                                             ttGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGTTAatcatc   <  2:1212573/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGTTAATCATCA  >  CP009273/3164085‑3164374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGTTAATCATCATGATG  >  CP009273/3164081‑3164379
                                                                                                                                                     |                                                                                                                                                     
ATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGA                                                                                                                                                       >  SRR19973069.3742752/1‑150 (MQ=60)
 TGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAG                                                                                                                                                      >  SRR19973069.2245744/1‑150 (MQ=60)
 TGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAG                                                                                                                                                      >  SRR19973069.15079/1‑150 (MQ=60)
  GTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGT                                                                                                                                                     >  SRR19973069.2573608/1‑150 (MQ=60)
     GATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTT                                                                                                                                                  <  SRR19973069.3292772/150‑1 (MQ=60)
      ATGTCGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGAGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGTAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTG                                                                                                                                                 <  SRR19973069.1768367/150‑1 (MQ=60)
      ATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTG                                                                                                                                                 <  SRR19973069.2431455/150‑1 (MQ=60)
        GACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTC                                                                                                                                               <  SRR19973069.2098747/150‑1 (MQ=60)
          CGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGATGAAGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTG                                                                                                                                             >  SRR19973069.2251239/1‑150 (MQ=60)
           GATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGG                                                                                                                                            >  SRR19973069.925139/1‑150 (MQ=60)
           GATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGG                                                                                                                                            >  SRR19973069.1864574/1‑150 (MQ=60)
             TGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGAT                                                                                                                                          <  SRR19973069.616485/150‑1 (MQ=60)
             TGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGAT                                                                                                                                          <  SRR19973069.1545359/150‑1 (MQ=60)
              GCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATG                                                                                                                                         <  SRR19973069.168070/150‑1 (MQ=60)
               CGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGA                                                                                                                                        <  SRR19973069.2217866/150‑1 (MQ=60)
                 CTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACC                                                                                                                                      >  SRR19973069.1494876/1‑150 (MQ=60)
                  TGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCT                                                                                                                                     >  SRR19973069.3708598/1‑150 (MQ=60)
                  TGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCT                                                                                                                                     >  SRR19973069.3708582/1‑150 (MQ=60)
                   GACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTC                                                                                                                                    <  SRR19973069.484520/150‑1 (MQ=60)
                    ACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCA                                                                                                                                   <  SRR19973069.3016336/150‑1 (MQ=60)
                     CCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCAC                                                                                                                                  >  SRR19973069.663743/1‑150 (MQ=60)
                     CCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCAC                                                                                                                                  >  SRR19973069.663905/1‑150 (MQ=60)
                           CCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATG                                                                                                                            >  SRR19973069.275848/1‑150 (MQ=60)
                            CATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGG                                                                                                                           <  SRR19973069.1380772/150‑1 (MQ=60)
                                 TTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAAT                                                                                                                      <  SRR19973069.2013589/150‑1 (MQ=60)
                                  ctCCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATA                                                                                                                     <  SRR19973069.625881/148‑1 (MQ=60)
                                  ctCCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATA                                                                                                                     <  SRR19973069.3109170/148‑1 (MQ=60)
                                   ACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATAT                                                                                                                    <  SRR19973069.1422500/150‑1 (MQ=60)
                                   ACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATAT                                                                                                                    >  SRR19973069.2633635/1‑150 (MQ=60)
                                    CCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATC                                                                                                                   >  SRR19973069.1739069/1‑150 (MQ=60)
                                    CCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATC                                                                                                                   >  SRR19973069.1738917/1‑150 (MQ=60)
                                            GGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTT                                                                                                           >  SRR19973069.3425257/1‑150 (MQ=60)
                                            GGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTT                                                                                                           <  SRR19973069.2680390/150‑1 (MQ=60)
                                            GGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTT                                                                                                           <  SRR19973069.2681059/150‑1 (MQ=60)
                                            GGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTT                                                                                                           <  SRR19973069.333853/150‑1 (MQ=60)
                                            GGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTT                                                                                                           <  SRR19973069.1028540/150‑1 (MQ=60)
                                             GCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTG                                                                                                          <  SRR19973069.1041532/150‑1 (MQ=60)
                                                  ATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGC                                                                                                     >  SRR19973069.2501045/1‑150 (MQ=60)
                                                   TGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCC                                                                                                    >  SRR19973069.2790272/1‑150 (MQ=60)
                                                   TGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCC                                                                                                    >  SRR19973069.2790271/1‑150 (MQ=60)
                                                     GTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAG                                                                                                  <  SRR19973069.1596525/150‑1 (MQ=60)
                                                       CCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGA                                                                                                <  SRR19973069.2254914/150‑1 (MQ=60)
                                                        CTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAC                                                                                               >  SRR19973069.3459123/1‑150 (MQ=60)
                                                        CTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAA                                                                                               >  SRR19973069.3458995/1‑150 (MQ=60)
                                                             TGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGA                                                                                          <  SRR19973069.2284406/150‑1 (MQ=60)
                                                             TGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGA                                                                                          <  SRR19973069.2284363/150‑1 (MQ=60)
                                                               ATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAAT                                                                                        >  SRR19973069.3828084/1‑150 (MQ=60)
                                                                 GGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATAC                                                                                      <  SRR19973069.2025308/150‑1 (MQ=60)
                                                                  GCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACC                                                                                     <  SRR19973069.417553/150‑1 (MQ=60)
                                                                   CGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCG                                                                                    >  SRR19973069.829080/1‑150 (MQ=60)
                                                                   CGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCG                                                                                    >  SRR19973069.829106/1‑150 (MQ=60)
                                                                    GATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGT                                                                                   >  SRR19973069.2130027/1‑150 (MQ=60)
                                                                        ACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAG                                                                               <  SRR19973069.1139177/150‑1 (MQ=60)
                                                                            AGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACAT                                                                           >  SRR19973069.3766/1‑150 (MQ=60)
                                                                              AAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGG                                                                         <  SRR19973069.379946/150‑1 (MQ=60)
                                                                              AAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGG                                                                         >  SRR19973069.239962/1‑150 (MQ=60)
                                                                               AGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGC                                                                        >  SRR19973069.2350360/1‑150 (MQ=60)
                                                                               AGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGC                                                                        >  SRR19973069.2350356/1‑150 (MQ=60)
                                                                                GATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCG                                                                       >  SRR19973069.168260/1‑150 (MQ=60)
                                                                                 ATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGC                                                                      >  SRR19973069.1920884/1‑150 (MQ=60)
                                                                                 ATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGC                                                                      >  SRR19973069.82371/1‑150 (MQ=60)
                                                                                      CCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCG                                                                 >  SRR19973069.2516401/1‑150 (MQ=60)
                                                                                        CTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGAT                                                               >  SRR19973069.912973/1‑150 (MQ=60)
                                                                                         TATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATT                                                              <  SRR19973069.2067268/150‑1 (MQ=60)
                                                                                          ATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGNCCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGGATACCGTGAAGACATGGCGCTGGCGATTG                                                             <  SRR19973069.1945170/150‑1 (MQ=60)
                                                                                          ATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTG                                                             >  SRR19973069.662443/1‑150 (MQ=60)
                                                                                          ATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTG                                                             <  SRR19973069.1071401/150‑1 (MQ=60)
                                                                                          ATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTG                                                             <  SRR19973069.2652643/150‑1 (MQ=60)
                                                                                            AGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTT                                                           <  SRR19973069.660668/150‑1 (MQ=60)
                                                                                            AGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTT                                                           >  SRR19973069.384007/1‑150 (MQ=60)
                                                                                            AGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTT                                                           <  SRR19973069.20610/150‑1 (MQ=60)
                                                                                               TATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCC                                                        >  SRR19973069.225535/1‑150 (MQ=60)
                                                                                               TATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCC                                                        <  SRR19973069.3323549/150‑1 (MQ=60)
                                                                                                     CGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAA                                                  >  SRR19973069.670109/1‑150 (MQ=60)
                                                                                                        GAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTG                                               <  SRR19973069.212721/150‑1 (MQ=60)
                                                                                                         AAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGA                                              <  SRR19973069.2699458/150‑1 (MQ=60)
                                                                                                         AAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGA                                              <  SRR19973069.2700200/150‑1 (MQ=60)
                                                                                                         AAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGA                                              >  SRR19973069.2043675/1‑150 (MQ=60)
                                                                                                           GGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATC                                            <  SRR19973069.3738314/150‑1 (MQ=60)
                                                                                                           GGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATC                                            <  SRR19973069.3738269/150‑1 (MQ=60)
                                                                                                           GGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATC                                            >  SRR19973069.350825/1‑150 (MQ=60)
                                                                                                                    GACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTG                                   <  SRR19973069.616332/150‑1 (MQ=60)
                                                                                                                    GACGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTG                                   <  SRR19973069.20717/150‑1 (MQ=60)
                                                                                                                      CGGGCAACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGT                                 <  SRR19973069.439535/150‑1 (MQ=60)
                                                                                                                           AACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCAC                            >  SRR19973069.2330388/1‑150 (MQ=60)
                                                                                                                           AACTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCAC                            >  SRR19973069.3614249/1‑150 (MQ=60)
                                                                                                                             CTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTG                          <  SRR19973069.755570/150‑1 (MQ=60)
                                                                                                                             CTGGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTG                          <  SRR19973069.755167/150‑1 (MQ=60)
                                                                                                                               GGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCC                        <  SRR19973069.1624840/150‑1 (MQ=60)
                                                                                                                               GGTCGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCC                        <  SRR19973069.1624845/150‑1 (MQ=60)
                                                                                                                                  CGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGAT                     <  SRR19973069.2472902/150‑1 (MQ=60)
                                                                                                                                  CGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGAT                     <  SRR19973069.2472730/150‑1 (MQ=60)
                                                                                                                                  CGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGAT                     <  SRR19973069.2472565/150‑1 (MQ=60)
                                                                                                                                  CGGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGAG                     >  SRR19973069.3424318/1‑150 (MQ=60)
                                                                                                                                   GGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATT                    >  SRR19973069.1422037/1‑150 (MQ=60)
                                                                                                                                   GGTGAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATT                    >  SRR19973069.3334618/1‑150 (MQ=60)
                                                                                                                                      GAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATG                 <  SRR19973069.3699424/150‑1 (MQ=60)
                                                                                                                                      GAAGACGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATG                 <  SRR19973069.3699413/150‑1 (MQ=60)
                                                                                                                                           CGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGTTAAT            <  SRR19973069.528552/150‑1 (MQ=60)
                                                                                                                                           CGATCCTTGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGTTAAT            >  SRR19973069.910387/1‑150 (MQ=60)
                                                                                                                                                  TGGAGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGTTAATCATCATG     <  SRR19973069.1263515/150‑1 (MQ=60)
                                                                                                                                                     AGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGTTAATCATCATGATG  >  SRR19973069.1225686/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
ATGTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAATGATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGGCAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAATATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTTGCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGTTAATCATCATGATG  >  CP009273/3164081‑3164379

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑