Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F121 I0 R1	856	273.6	9081716	94.1%	8545894	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,770,281	T→A	83.8%	D146E (GAT→GAA)	ydiF →	putative acetyl‑CoA:acetoacetyl‑CoA transferase: alpha subunit/beta subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,770,281	0	T	A	83.8%	712.3 / 121.7	252	D146E (GAT→GAA)	ydiF	putative acetyl‑CoA:acetoacetyl‑CoA transferase: alpha subunit/beta subunit
Reads supporting (aligned to +/- strand): ref base T (25/16); new base A (107/104); total (132/120)
Fisher's exact test for biased strand distribution p-value = 2.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 252 total aligned reads displayed.

ACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGG  >  CP009273/1770134‑1770427

aCAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATcc                                                                                                                                                  <  1:1624202/150‑1 (MQ=255)

   aTCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACg                                                                                                                                               <  1:4401802/150‑1 (MQ=255)

        cgcgTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGc                                                                                                                                          >  2:1875460/1‑150 (MQ=255)

           gTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAg                                                                                                                                       <  2:1788108/150‑1 (MQ=255)

                    aaCTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAAc                                                                                                                              >  1:3366275/1‑150 (MQ=255)

                        cGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACtgaa                                                                                                                          >  2:3211918/1‑150 (MQ=255)

                          cAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACtgaatg                                                                                                                        >  1:3937354/1‑150 (MQ=255)

                           aGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACtgaatga                                                                                                                       >  1:244121/1‑150 (MQ=255)

                             aaCAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAg                                                                                                                     >  1:2850656/1‑150 (MQ=255)

                             aaCAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAg                                                                                                                     >  2:1580285/1‑150 (MQ=255)

                               cAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTc                                                                                                                   <  2:4025601/150‑1 (MQ=255)

                                aaaataaaatTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCa                                                                                                                  <  2:3752790/150‑1 (MQ=255)

                                aaaataaaatTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCa                                                                                                                  <  2:3142196/150‑1 (MQ=255)

                                 aaataaaatTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCAc                                                                                                                 >  2:719360/1‑150 (MQ=255)

                                 aaataaaatTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCAc                                                                                                                 >  1:388469/1‑150 (MQ=255)

                                   ataaaatTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTa                                                                                                               <  2:1393993/150‑1 (MQ=255)

                                     aaaatTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTaaa                                                                                                             >  2:877834/1‑150 (MQ=255)

                                     aaaatTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTaaa                                                                                                             >  2:3034702/1‑150 (MQ=255)

                                      aaatTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAaag                                                                                                            >  1:4079342/1‑150 (MQ=255)

                                      aaatTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAaag                                                                                                            <  1:1099420/150‑1 (MQ=255)

                                      aaatTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAaag                                                                                                            <  1:2960203/150‑1 (MQ=255)

                                       aatTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAaaga                                                                                                           >  1:1286009/1‑150 (MQ=255)

                                       aatTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAaaga                                                                                                           <  2:2706749/150‑1 (MQ=255)

                                        attattGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAaagaa                                                                                                          >  1:434788/1‑150 (MQ=255)

                                        attattGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAaagaa                                                                                                          <  2:398938/150‑1 (MQ=255)

                                          tattGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAagaaga                                                                                                        <  1:4460517/150‑1 (MQ=255)

                                           attGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGAc                                                                                                       >  1:377666/1‑150 (MQ=255)

                                            ttGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGAcc                                                                                                      >  1:1709762/1‑150 (MQ=255)

                                             tGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCt                                                                                                     <  2:921122/150‑1 (MQ=255)

                                             tGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCt                                                                                                     <  1:3360967/150‑1 (MQ=255)

                                             tGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCt                                                                                                     <  1:3675668/150‑1 (MQ=255)

                                               cTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGa                                                                                                   <  2:1720894/150‑1 (MQ=255)

                                               cTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGa                                                                                                   >  2:2419401/1‑150 (MQ=255)

                                               cTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGa                                                                                                   >  2:2837011/1‑150 (MQ=255)

                                                   taACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTaa                                                                                               >  2:4453711/1‑150 (MQ=255)

                                                     aCTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAAc                                                                                             >  1:1488546/1‑150 (MQ=255)

                                                     aCTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAAc                                                                                             >  2:2106794/1‑150 (MQ=255)

                                                      cTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACt                                                                                            >  1:2119532/1‑150 (MQ=255)

                                                      cTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACt                                                                                            >  1:8722/1‑150 (MQ=255)

                                                      cTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACt                                                                                            >  1:32720/1‑150 (MQ=255)

                                                       tACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTg                                                                                           >  1:2519106/1‑150 (MQ=255)

                                                       tACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTg                                                                                           >  2:4380193/1‑150 (MQ=255)

                                                       tACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTg                                                                                           >  2:3016842/1‑150 (MQ=255)

                                                        aCCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTgg                                                                                          >  1:1671124/1‑150 (MQ=255)

                                                         cccACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGt                                                                                         >  2:308039/1‑150 (MQ=255)

                                                          ccACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTc                                                                                        >  2:1248622/1‑150 (MQ=255)

                                                           cacaAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCg                                                                                       >  2:2519811/1‑150 (MQ=255)

                                                           cacaAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCg                                                                                       <  2:3403655/150‑1 (MQ=255)

                                                             caAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAg                                                                                     >  2:807373/1‑150 (MQ=255)

                                                                ggTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGttt                                                                                  >  2:3731897/1‑150 (MQ=255)

                                                                ggTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGttt                                                                                  <  1:518120/150‑1 (MQ=255)

                                                                 gtgtACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTg                                                                                 <  2:149863/150‑1 (MQ=255)

                                                                   gtACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGAt                                                                               <  1:1174701/150‑1 (MQ=255)

                                                                   gtACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGAt                                                                               <  1:3876306/150‑1 (MQ=255)

                                                                   gtACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGAt                                                                               <  1:1825/150‑1 (MQ=255)

                                                                     aCTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATaa                                                                             >  1:3527710/1‑150 (MQ=255)

                                                                     aCTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATaa                                                                             <  1:2061281/150‑1 (MQ=255)

                                                                     aCTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATaa                                                                             <  2:3316269/150‑1 (MQ=255)

                                                                        tACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACaa                                                                          >  2:1324326/1‑150 (MQ=255)

                                                                         acacAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACaaa                                                                         <  1:4519682/150‑1 (MQ=255)

                                                                          cacaAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACTTTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAg                                                                        <  1:2537643/150‑1 (MQ=255)

                                                                           acaAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGa                                                                       <  2:194015/150‑1 (MQ=255)

                                                                             aaaCCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAat                                                                     <  2:1349455/150‑1 (MQ=255)

                                                                               aCCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAatat                                                                   <  1:4366805/150‑1 (MQ=255)

                                                                                   tACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATctct                                                               >  2:3877392/1‑150 (MQ=255)

                                                                                   tACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATctct                                                               >  2:222041/1‑150 (MQ=255)

                                                                                   tACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATctct                                                               <  1:4022293/150‑1 (MQ=255)

                                                                                     cgcgCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTAt                                                             <  2:2360291/150‑1 (MQ=255)

                                                                                             cgcAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGc                                                     >  2:1861270/1‑150 (MQ=255)

                                                                                             cgcAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGc                                                     >  2:3841863/1‑150 (MQ=255)

                                                                                             cgcAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGc                                                     >  2:2304525/1‑150 (MQ=255)

                                                                                              gcAGCCCACCAGCCTGTTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCg                                                    <  2:4246864/150‑1 (MQ=255)

                                                                                                aGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGAt                                                  >  1:1604260/1‑150 (MQ=255)

                                                                                                aGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGAt                                                  <  1:1891342/150‑1 (MQ=255)

                                                                                                       caGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCa                                           <  1:235586/150‑1 (MQ=255)

                                                                                                        aGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAg                                          >  2:1897361/1‑150 (MQ=255)

                                                                                                          ccTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGat                                        >  2:2603446/1‑150 (MQ=255)

                                                                                                          ccTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGat                                        <  1:504118/150‑1 (MQ=255)

                                                                                                          ccTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGat                                        >  2:1142973/1‑150 (MQ=255)

                                                                                                           cTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGata                                       >  1:2115200/1‑150 (MQ=255)

                                                                                                            tGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGatat                                      <  1:815887/150‑1 (MQ=255)

                                                                                                                 ttattaGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCt                                 >  2:2114110/1‑150 (MQ=255)

                                                                                                                   attaGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTc                               <  1:2700501/150‑1 (MQ=255)

                                                                                                                     taGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCAt                             <  1:4022933/150‑1 (MQ=255)

                                                                                                                      aGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCAtt                            >  1:4068006/1‑150 (MQ=255)

                                                                                                                      aGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCAtt                            >  1:4251639/1‑150 (MQ=255)

                                                                                                                      aGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCAtt                            >  1:1521229/1‑150 (MQ=255)

                                                                                                                       gTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTc                           <  1:3011066/150‑1 (MQ=255)

                                                                                                                          atatTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTcgcg                        >  1:4418947/1‑150 (MQ=255)

                                                                                                                            atTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCt                      <  2:1158928/150‑1 (MQ=255)

                                                                                                                            atTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCt                      <  1:1256019/150‑1 (MQ=255)

                                                                                                                            atTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCt                      <  2:2919000/150‑1 (MQ=255)

                                                                                                                                   tCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCt               <  2:618179/150‑1 (MQ=255)

                                                                                                                                   tCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCt               <  1:3863299/150‑1 (MQ=255)

                                                                                                                                    cGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTg              >  1:705082/1‑150 (MQ=255)

                                                                                                                                    cGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTg              >  1:3466903/1‑150 (MQ=255)

                                                                                                                                      ggACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCg            <  1:310468/150‑1 (MQ=255)

                                                                                                                                      ggACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCg            >  1:1835838/1‑150 (MQ=255)

                                                                                                                                         cATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACa         <  1:4259922/150‑1 (MQ=255)

                                                                                                                                                cGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAgg  >  1:3177318/1‑150 (MQ=255)

ACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGG  >  CP009273/1770134‑1770427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGG  >  CP009273/1770134‑1770427

ACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCC                                                                                                                                                  <  SRR19973080.1633713/150‑1 (MQ=60)

ACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACC                                                                                                                                                  <  SRR19973080.1633718/150‑1 (MQ=60)

ACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACC                                                                                                                                                  <  SRR19973080.1633617/150‑1 (MQ=60)

   ATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACG                                                                                                                                               <  SRR19973080.4425826/150‑1 (MQ=60)

   ATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACG                                                                                                                                               <  SRR19973080.4425757/150‑1 (MQ=60)

      GCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCA                                                                                                                                            <  SRR19973080.3007956/150‑1 (MQ=60)

       CCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAG                                                                                                                                           <  SRR19973080.188349/150‑1 (MQ=60)

            TATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGG                                                                                                                                      >  SRR19973080.151082/1‑150 (MQ=60)

               TTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGG                                                                                                                                   >  SRR19973080.3976327/1‑150 (MQ=60)

                 CTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCA                                                                                                                                 <  SRR19973080.209029/150‑1 (MQ=60)

                   GAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAA                                                                                                                               >  SRR19973080.520565/1‑150 (MQ=60)

                    AACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAAC                                                                                                                              >  SRR19973080.3384530/1‑150 (MQ=60)

                      CTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTG                                                                                                                            >  SRR19973080.2582723/1‑150 (MQ=60)

                      CTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTG                                                                                                                            <  SRR19973080.434119/150‑1 (MQ=60)

                          CAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATG                                                                                                                        >  SRR19973080.3959072/1‑150 (MQ=60)

                          CAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATG                                                                                                                        >  SRR19973080.4073239/1‑150 (MQ=60)

                           AGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGA                                                                                                                       >  SRR19973080.245806/1‑150 (MQ=60)

                             AACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCTATCCACGCCAGCAAGGCGGCAAACTGAATGAAG                                                                                                                     >  SRR19973080.4519913/1‑150 (MQ=60)

                             AACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAG                                                                                                                     >  SRR19973080.2866296/1‑150 (MQ=60)

                              ACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGT                                                                                                                    >  SRR19973080.998309/1‑150 (MQ=60)

                               CAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTC                                                                                                                   >  SRR19973080.3086398/1‑150 (MQ=60)

                                 AAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCAC                                                                                                                 >  SRR19973080.390710/1‑150 (MQ=60)

                                 AAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCAC                                                                                                                 >  SRR19973080.3967268/1‑150 (MQ=60)

                                   ATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTA                                                                                                               >  SRR19973080.1770773/1‑150 (MQ=60)

                                    TAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAA                                                                                                              >  SRR19973080.3355359/1‑150 (MQ=60)

                                      AAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAG                                                                                                            <  SRR19973080.3791330/150‑1 (MQ=60)

                                      AAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAG                                                                                                            <  SRR19973080.2976242/150‑1 (MQ=60)

                                      AAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAG                                                                                                            >  SRR19973080.3909652/1‑150 (MQ=60)

                                      AAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAG                                                                                                            >  SRR19973080.4101587/1‑150 (MQ=60)

                                      AAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAG                                                                                                            <  SRR19973080.1105752/150‑1 (MQ=60)

                                      AAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAG                                                                                                            <  SRR19973080.1105749/150‑1 (MQ=60)

                                       AATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGA                                                                                                           >  SRR19973080.1293783/1‑150 (MQ=60)

                                       AATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGA                                                                                                           >  SRR19973080.1292439/1‑150 (MQ=60)

                                        ATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAA                                                                                                          >  SRR19973080.1553546/1‑150 (MQ=60)

                                        ATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAA                                                                                                          <  SRR19973080.3185188/150‑1 (MQ=60)

                                        ATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAA                                                                                                          >  SRR19973080.437484/1‑150 (MQ=60)

                                          TATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGA                                                                                                        <  SRR19973080.4484690/150‑1 (MQ=60)

                                          TATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGA                                                                                                        <  SRR19973080.4484615/150‑1 (MQ=60)

                                           ATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGACTGAAGTCACTAAAGAAGAC                                                                                                       >  SRR19973080.4324203/1‑150 (MQ=60)

                                           ATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGAC                                                                                                       >  SRR19973080.379803/1‑150 (MQ=60)

                                            TTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACC                                                                                                      >  SRR19973080.1719269/1‑150 (MQ=60)

                                            TTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACC                                                                                                      >  SRR19973080.1719692/1‑150 (MQ=60)

                                             TGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCT                                                                                                     <  SRR19973080.3695901/150‑1 (MQ=60)

                                             TGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCT                                                                                                     <  SRR19973080.3379232/150‑1 (MQ=60)

                                             TGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCT                                                                                                     <  SRR19973080.3379205/150‑1 (MQ=60)

                                                   TAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAA                                                                                               >  SRR19973080.2906323/1‑150 (MQ=60)

                                                     ACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAAC                                                                                             <  SRR19973080.3899841/150‑1 (MQ=60)

                                                     ACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAAC                                                                                             <  SRR19973080.1843189/150‑1 (MQ=60)

                                                     ACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAAC                                                                                             <  SRR19973080.3899875/150‑1 (MQ=60)

                                                     ACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAAC                                                                                             >  SRR19973080.1497046/1‑150 (MQ=60)

                                                      CTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACT                                                                                            >  SRR19973080.8820/1‑150 (MQ=60)

                                                      CTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACT                                                                                            >  SRR19973080.2131489/1‑150 (MQ=60)

                                                      CTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACT                                                                                            >  SRR19973080.33070/1‑150 (MQ=60)

                                                      CTACACACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATAATGGCATCGGGACATTTGTCTATCCACGCAAGCAAGGAGGCAAACTGAATGAAGTCACTAAAGAATACCTGATTAAACT                                                                                            >  SRR19973080.2130175/1‑150 (MQ=60)

                                                       TACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG                                                                                           >  SRR19973080.2532974/1‑150 (MQ=60)

                                                        ACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGG                                                                                          >  SRR19973080.1680885/1‑150 (MQ=60)

                                                           CACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCG                                                                                       <  SRR19973080.563668/150‑1 (MQ=60)

                                                           CACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCG                                                                                       <  SRR19973080.4083394/150‑1 (MQ=60)

                                                            ACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGA                                                                                      >  SRR19973080.51784/1‑150 (MQ=60)

                                                               AGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTT                                                                                   >  SRR19973080.2535245/1‑150 (MQ=60)

                                                                GGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTT                                                                                  <  SRR19973080.521424/150‑1 (MQ=60)

                                                                   GTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGAT                                                                               <  SRR19973080.1848/150‑1 (MQ=60)

                                                                   GTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGAT                                                                               <  SRR19973080.1181701/150‑1 (MQ=60)

                                                                   GTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGAT                                                                               <  SRR19973080.1680163/150‑1 (MQ=60)

                                                                   GTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGAT                                                                               <  SRR19973080.3897660/150‑1 (MQ=60)

                                                                   GTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGAT                                                                               <  SRR19973080.3480690/150‑1 (MQ=60)

                                                                   GTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGAT                                                                               <  SRR19973080.3896466/150‑1 (MQ=60)

                                                                     ACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAA                                                                             >  SRR19973080.1249069/1‑150 (MQ=60)

                                                                     ACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAA                                                                             >  SRR19973080.3547220/1‑150 (MQ=60)

                                                                     ACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAA                                                                             <  SRR19973080.2072971/150‑1 (MQ=60)

                                                                       TTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACA                                                                           >  SRR19973080.1749142/1‑150 (MQ=60)

                                                                         ACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAA                                                                         <  SRR19973080.4544046/150‑1 (MQ=60)

                                                                          CACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACTTTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAG                                                                        <  SRR19973080.2551565/150‑1 (MQ=60)

                                                                           ACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGA                                                                       <  SRR19973080.328923/150‑1 (MQ=60)

                                                                               ACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATAT                                                                   <  SRR19973080.4390627/150‑1 (MQ=60)

                                                                               ACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATAT                                                                   <  SRR19973080.4390621/150‑1 (MQ=60)

                                                                               ACCGTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATAT                                                                   <  SRR19973080.1916101/150‑1 (MQ=60)

                                                                                   TACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCT                                                               <  SRR19973080.4044377/150‑1 (MQ=60)

                                                                                   TACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCT                                                               <  SRR19973080.4044340/150‑1 (MQ=60)

                                                                                         CCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACA                                                         <  SRR19973080.3808971/150‑1 (MQ=60)

                                                                                          CGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAA                                                        >  SRR19973080.2229104/1‑150 (MQ=60)

                                                                                           GCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAA                                                       <  SRR19973080.858004/150‑1 (MQ=60)

                                                                                            CCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAG                                                      <  SRR19973080.1400824/150‑1 (MQ=60)

                                                                                             CGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGC                                                     <  SRR19973080.2649406/150‑1 (MQ=60)

                                                                                              GCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCG                                                    >  SRR19973080.2842691/1‑150 (MQ=60)

                                                                                                AGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGAT                                                  <  SRR19973080.1901905/150‑1 (MQ=60)

                                                                                                AGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGAT                                                  >  SRR19973080.1613461/1‑150 (MQ=60)

                                                                                                AGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGAT                                                  >  SRR19973080.1613649/1‑150 (MQ=60)

                                                                                                 GCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATT                                                 <  SRR19973080.3414896/150‑1 (MQ=60)

                                                                                                 GCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGAAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGTATATCTCTATTACAAAGCGATT                                                 <  SRR19973080.2493797/150‑1 (MQ=60)

                                                                                                  CCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTG                                                <  SRR19973080.3301730/150‑1 (MQ=60)

                                                                                                  CCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACGGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTG                                                <  SRR19973080.3814766/150‑1 (MQ=60)

                                                                                                   CCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGC                                               <  SRR19973080.723107/150‑1 (MQ=60)

                                                                                                    CACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCG                                              <  SRR19973080.2520954/150‑1 (MQ=60)

                                                                                                     ACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGC                                             >  SRR19973080.2105278/1‑150 (MQ=60)

                                                                                                     ACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGC                                             >  SRR19973080.4387916/1‑150 (MQ=60)

                                                                                                       CAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCA                                           <  SRR19973080.237238/150‑1 (MQ=60)

                                                                                                        AGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAG                                          >  SRR19973080.4448039/1‑150 (MQ=60)

                                                                                                          CCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGAT                                        <  SRR19973080.507239/150‑1 (MQ=60)

                                                                                                          CCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGAT                                        <  SRR19973080.507331/150‑1 (MQ=60)

                                                                                                          ACTGGTATTATTAGTTATATTGGCATCTGGACATTTGTCTAACCACGACAGCAATGCGGAAAAATGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGAT                                        <  SRR19973080.2429060/150‑1 (MQ=60)

                                                                                                           CTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATA                                       >  SRR19973080.2127137/1‑150 (MQ=60)

                                                                                                            TGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATAT                                      <  SRR19973080.820730/150‑1 (MQ=60)

                                                                                                            TGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATAT                                      <  SRR19973080.3556774/150‑1 (MQ=60)

                                                                                                            TGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATAT                                      <  SRR19973080.1886493/150‑1 (MQ=60)

                                                                                                              GTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTG                                    >  SRR19973080.2253788/1‑150 (MQ=60)

                                                                                                                   ATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTC                               <  SRR19973080.2715283/150‑1 (MQ=60)

                                                                                                                   ATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTC                               >  SRR19973080.3499781/1‑150 (MQ=60)

                                                                                                                   ATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTC                               >  SRR19973080.1199674/1‑150 (MQ=60)

                                                                                                                     TAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCAT                             <  SRR19973080.4044984/150‑1 (MQ=60)

                                                                                                                      AGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATT                            >  SRR19973080.4090211/1‑150 (MQ=60)

                                                                                                                      AGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATT                            >  SRR19973080.4090427/1‑150 (MQ=60)

                                                                                                                      AGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATT                            >  SRR19973080.4274891/1‑150 (MQ=60)

                                                                                                                      AGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATT                            >  SRR19973080.1529953/1‑150 (MQ=60)

                                                                                                                      AGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATT                            >  SRR19973080.4274849/1‑150 (MQ=60)

                                                                                                                       GTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTC                           <  SRR19973080.3027253/150‑1 (MQ=60)

                                                                                                                          ATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCG                        >  SRR19973080.4442977/1‑150 (MQ=60)

                                                                                                                            ATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCT                      >  SRR19973080.1469860/1‑150 (MQ=60)

                                                                                                                            ATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCT                      <  SRR19973080.1263634/150‑1 (MQ=60)

                                                                                                                                   TCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCT               <  SRR19973080.3884568/150‑1 (MQ=60)

                                                                                                                                    CGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTCCCACCTG              >  SRR19973080.4145294/1‑150 (MQ=60)

                                                                                                                                    CGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTG              >  SRR19973080.809253/1‑150 (MQ=60)

                                                                                                                                    CGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTG              >  SRR19973080.3485918/1‑150 (MQ=60)

                                                                                                                                    CGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTG              >  SRR19973080.709188/1‑150 (MQ=60)

                                                                                                                                     GGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTCCCACCTGC             >  SRR19973080.488774/1‑150 (MQ=60)

                                                                                                                                      GGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCG            <  SRR19973080.3633664/150‑1 (MQ=60)

                                                                                                                                      GGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCG            <  SRR19973080.312423/150‑1 (MQ=60)

                                                                                                                                      GGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCG            >  SRR19973080.1846184/1‑150 (MQ=60)

                                                                                                                                      GGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCG            >  SRR19973080.4135223/1‑150 (MQ=60)

                                                                                                                                         CATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACA         <  SRR19973080.4283163/150‑1 (MQ=60)

                                                                                                                                          ATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAG        >  SRR19973080.2919356/1‑150 (MQ=60)

                                                                                                                                          ATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAG        >  SRR19973080.965245/1‑150 (MQ=60)

                                                                                                                                             TGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGA     <  SRR19973080.4415006/150‑1 (MQ=60)

                                                                                                                                                CGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGG  >  SRR19973080.3194840/1‑150 (MQ=60)

                                                                                                                                                CGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGG  >  SRR19973080.3194844/1‑150 (MQ=60)

                                                                                                                                                CGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGG  <  SRR19973080.3053379/150‑1 (MQ=60)

ACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGG  >  CP009273/1770134‑1770427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`