Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,770,281 T→A 100% D146E (GAT→GAA)  ydiF → putative acetyl‑CoA:acetoacetyl‑CoA transferase: alpha subunit/beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,770,2810TA99.6% 752.3 / ‑4.6 226D146E (GAT→GAA) ydiFputative acetyl‑CoA:acetoacetyl‑CoA transferase: alpha subunit/beta subunit
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base A (121/104);  total (122/104)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 226 total aligned reads displayed.

AATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAA  >  CP009273/1770136‑1770425
                                                                                                                                                  |                                                                                                                                                
  tCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCAc                                                                                                                                              >  2:3398741/1‑148 (MQ=255)
   cgccgcGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACg                                                                                                                                             >  1:1352757/1‑148 (MQ=255)
    gccgcGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGc                                                                                                                                            <  2:728371/148‑1 (MQ=255)
    gccgaGTATTTCAGACCTCGAAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCTCCAGCCTGGTATTATTAGTTATATTGGCATCGGGACA‑TTTGTCGAACCCCGc                                                                                                                                            <  2:2910451/148‑1 (MQ=255)
         gTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCa                                                                                                                                       <  2:1582604/148‑1 (MQ=255)
         gTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCa                                                                                                                                       <  1:1249915/148‑1 (MQ=255)
            tttCTGACCTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAgg                                                                                                                                    <  2:1273505/148‑1 (MQ=255)
            tttCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAgg                                                                                                                                    <  2:1934173/148‑1 (MQ=255)
                    cTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAAc                                                                                                                            >  1:1398299/1‑148 (MQ=255)
                     tCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACt                                                                                                                           >  1:1005244/1‑148 (MQ=255)
                      cGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACtg                                                                                                                          >  1:1529436/1‑148 (MQ=255)
                         aGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACtgaat                                                                                                                       >  1:740315/1‑148 (MQ=255)
                          gAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACtgaatg                                                                                                                      >  2:3358159/1‑148 (MQ=255)
                          gAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACtgaatg                                                                                                                      >  2:3205693/1‑148 (MQ=255)
                          gAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACtgaatg                                                                                                                      >  2:3024178/1‑148 (MQ=255)
                            aCAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACtgaatgaa                                                                                                                    <  2:572657/148‑1 (MQ=255)
                             cAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAg                                                                                                                   >  1:3255990/1‑148 (MQ=255)
                             cAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAg                                                                                                                   <  2:2747096/148‑1 (MQ=255)
                              aaaataaaatTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGt                                                                                                                  >  1:2060921/1‑148 (MQ=255)
                               aaataaaatTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTc                                                                                                                 >  1:3044659/1‑148 (MQ=255)
                                  taaaatTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGCACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACt                                                                                                              >  2:226730/1‑148 (MQ=255)
                                   aaaatTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTa                                                                                                             >  1:2264521/1‑148 (MQ=255)
                                    aaatTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTaa                                                                                                            >  2:1716353/1‑148 (MQ=255)
                                    aaatTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTaa                                                                                                            <  2:2163371/148‑1 (MQ=255)
                                    aaatTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTaa                                                                                                            >  1:2613975/1‑148 (MQ=255)
                                    aaatTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTAGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTaa                                                                                                            <  1:2411111/148‑1 (MQ=255)
                                     aatTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTaaa                                                                                                           >  1:889346/1‑148 (MQ=255)
                                     aatTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTaaa                                                                                                           >  2:3253901/1‑148 (MQ=255)
                                       ttattGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAaaga                                                                                                         >  2:2278546/1‑148 (MQ=255)
                                       ttattGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAaaga                                                                                                         >  2:1595366/1‑148 (MQ=255)
                                         attGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAaagaag                                                                                                       >  2:1346786/1‑148 (MQ=255)
                                         attGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAaagaag                                                                                                       <  1:1622101/148‑1 (MQ=255)
                                          ttGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAagaaga                                                                                                      <  1:615672/148‑1 (MQ=255)
                                          ttGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAagaaga                                                                                                      >  1:890983/1‑148 (MQ=255)
                                           tGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGAc                                                                                                     <  1:1514418/148‑1 (MQ=255)
                                           tGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGAc                                                                                                     <  1:1280858/148‑1 (MQ=255)
                                               tataACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGa                                                                                                 >  1:3215332/1‑148 (MQ=255)
                                               tataACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGa                                                                                                 >  1:1727483/1‑148 (MQ=255)
                                                ataACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGAt                                                                                                >  2:1048999/1‑148 (MQ=255)
                                                ataACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGAt                                                                                                >  2:10311/1‑148 (MQ=255)
                                                 taACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGAtt                                                                                               >  2:554258/1‑148 (MQ=255)
                                                    cTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTaaa                                                                                            >  2:2680152/1‑148 (MQ=255)
                                                     tACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAAc                                                                                           <  1:1471840/148‑1 (MQ=255)
                                                      aCCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACt                                                                                          >  2:521976/1‑148 (MQ=255)
                                                       cccACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTg                                                                                         >  1:3162694/1‑148 (MQ=255)
                                                       cccACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTg                                                                                         >  1:118867/1‑148 (MQ=255)
                                                       cccACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTg                                                                                         >  2:1529973/1‑148 (MQ=255)
                                                           caAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCg                                                                                     <  2:1478610/148‑1 (MQ=255)
                                                           caAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCg                                                                                     <  2:899918/148‑1 (MQ=255)
                                                               gtgtACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGtt                                                                                 <  1:2896197/148‑1 (MQ=255)
                                                                 gtACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTg                                                                               <  1:2712448/148‑1 (MQ=255)
                                                                 gtACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTg                                                                               <  1:1494510/148‑1 (MQ=255)
                                                                 gtACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTg                                                                               >  2:522595/1‑148 (MQ=255)
                                                                 gtACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTg                                                                               >  1:1173238/1‑148 (MQ=255)
                                                                  tACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGa                                                                              <  2:1524072/148‑1 (MQ=255)
                                                                   aCTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGAt                                                                             <  1:3238145/148‑1 (MQ=255)
                                                                   aCTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGAt                                                                             <  1:3469641/148‑1 (MQ=255)
                                                                     ttACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATaa                                                                           <  2:251615/148‑1 (MQ=255)
                                                                     ttACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATaa                                                                           <  1:3513538/148‑1 (MQ=255)
                                                                       acacAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAAc                                                                          >  1:765202/1‑148 (MQ=255)
                                                                         acaAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTNAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACaaa                                                                       <  1:2837938/148‑1 (MQ=255)
                                                                          caAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAg                                                                      <  2:2372282/148‑1 (MQ=255)
                                                                            aaCCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGaa                                                                    >  1:463542/1‑148 (MQ=255)
                                                                              ccTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAata                                                                  >  1:1614396/1‑148 (MQ=255)
                                                                                 tACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATAtct                                                               <  2:2608973/148‑1 (MQ=255)
                                                                                   cgcgCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATctct                                                             <  2:1641788/148‑1 (MQ=255)
                                                                                   cgcgCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATctct                                                             <  2:1946404/148‑1 (MQ=255)
                                                                                     cgccgccgcAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTAt                                                           <  2:2262521/148‑1 (MQ=255)
                                                                                     cgccgccgcAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTAt                                                           <  2:2262549/148‑1 (MQ=255)
                                                                                        cgccgcAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTAc                                                        >  1:3357534/1‑148 (MQ=255)
                                                                                          ccgcAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACaa                                                      >  1:1471670/1‑148 (MQ=255)
                                                                                          ccgcAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACaa                                                      >  2:220506/1‑148 (MQ=255)
                                                                                            gcAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAg                                                    <  1:1425703/148‑1 (MQ=255)
                                                                                                 ccaccaGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGAtt                                               <  2:488498/148‑1 (MQ=255)
                                                                                                  caccaGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTg                                              <  2:2100539/148‑1 (MQ=255)
                                                                                                  caccaGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTg                                              <  1:1773796/148‑1 (MQ=255)
                                                                                                    ccaGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTgcg                                            >  2:1149529/1‑148 (MQ=255)
                                                                                                        ccTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAg                                        <  1:1108519/148‑1 (MQ=255)
                                                                                                           ggTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGata                                     >  2:3201766/1‑148 (MQ=255)
                                                                                                            gTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGatat                                    <  1:377385/148‑1 (MQ=255)
                                                                                                            gTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGatat                                    >  2:879135/1‑148 (MQ=255)
                                                                                                              attattAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTg                                  >  1:1058550/1‑148 (MQ=255)
                                                                                                              attattAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTg                                  >  1:1919066/1‑148 (MQ=255)
                                                                                                              attattAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTg                                  >  1:3127950/1‑148 (MQ=255)
                                                                                                                tattaGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGcc                                >  2:3181242/1‑148 (MQ=255)
                                                                                                                tattaGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGcc                                >  1:2316385/1‑148 (MQ=255)
                                                                                                                tattaGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGcc                                >  2:2128034/1‑148 (MQ=255)
                                                                                                                  ttaGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCtt                              >  1:53283/1‑148 (MQ=255)
                                                                                                                     gTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCAt                           >  2:303394/1‑148 (MQ=255)
                                                                                                                      tGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCAtt                          >  1:2962745/1‑148 (MQ=255)
                                                                                                                      tGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCAtt                          >  1:2999860/1‑148 (MQ=255)
                                                                                                                        atatTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTcg                        <  1:2278546/148‑1 (MQ=255)
                                                                                                                        atatTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTcg                        <  1:1595065/148‑1 (MQ=255)
                                                                                                                          atTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTcgcg                      >  2:3553605/1‑148 (MQ=255)
                                                                                                                          atTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTcgcg                      <  2:1529436/148‑1 (MQ=255)
                                                                                                                             ggCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTa                   <  2:2964620/148‑1 (MQ=255)
                                                                                                                              gCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTac                  <  1:1960827/148‑1 (MQ=255)
                                                                                                                                  cGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTaccacc              >  1:621502/1‑148 (MQ=255)
                                                                                                                                        a‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGAc        >  1:694424/1‑148 (MQ=255)
                                                                                                                                              tCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGa   >  2:1197066/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
AATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAA  >  CP009273/1770136‑1770425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCT  >  CP009273/1770132‑1770429
                                                                                                                                                      |                                                                                                                                                    
GGACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAA                                                                                                                                                      <  SRR19973067.956861/150‑1 (MQ=60)
GGACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAA                                                                                                                                                      <  SRR19973067.956675/150‑1 (MQ=60)
 GACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAAC                                                                                                                                                     <  SRR19973067.3339452/150‑1 (MQ=60)
  ACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACAACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACC                                                                                                                                                    >  SRR19973067.1623183/1‑150 (MQ=60)
    AATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCAC                                                                                                                                                  >  SRR19973067.3220246/1‑150 (MQ=60)
     ATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACG                                                                                                                                                 >  SRR19973067.1362002/1‑150 (MQ=60)
          CGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGC                                                                                                                                            >  SRR19973067.762339/1‑150 (MQ=60)
             GTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAG                                                                                                                                         <  SRR19973067.1258354/150‑1 (MQ=60)
              TATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGG                                                                                                                                        >  SRR19973067.62607/1‑150 (MQ=60)
                TTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCG                                                                                                                                      <  SRR19973067.2485271/150‑1 (MQ=60)
                      AACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAAC                                                                                                                                >  SRR19973067.3290950/1‑150 (MQ=60)
                      AACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAAC                                                                                                                                >  SRR19973067.1407788/1‑150 (MQ=60)
                      AACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAAC                                                                                                                                >  SRR19973067.1407791/1‑150 (MQ=60)
                       ACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACT                                                                                                                               >  SRR19973067.1012147/1‑150 (MQ=60)
                        CTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTG                                                                                                                              >  SRR19973067.1539880/1‑150 (MQ=60)
                        CTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTG                                                                                                                              >  SRR19973067.1539747/1‑150 (MQ=60)
                           GCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAAT                                                                                                                           >  SRR19973067.745450/1‑150 (MQ=60)
                            CAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATG                                                                                                                          >  SRR19973067.201391/1‑150 (MQ=60)
                               AACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAG                                                                                                                       >  SRR19973067.3276337/1‑150 (MQ=60)
                               AACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAG                                                                                                                       >  SRR19973067.3276343/1‑150 (MQ=60)
                               AACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAG                                                                                                                       >  SRR19973067.2177566/1‑150 (MQ=60)
                               AACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAG                                                                                                                       <  SRR19973067.725624/150‑1 (MQ=60)
                               AACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAG                                                                                                                       <  SRR19973067.2964208/150‑1 (MQ=60)
                               AACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAG                                                                                                                       >  SRR19973067.1420772/1‑150 (MQ=60)
                               AACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAG                                                                                                                       <  SRR19973067.1181257/150‑1 (MQ=60)
                                ACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGT                                                                                                                      >  SRR19973067.2074647/1‑150 (MQ=60)
                                 CAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTC                                                                                                                     <  SRR19973067.857408/150‑1 (MQ=60)
                                 CAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTC                                                                                                                     >  SRR19973067.3064052/1‑150 (MQ=60)
                                  AAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCA                                                                                                                    <  SRR19973067.146566/150‑1 (MQ=60)
                                   AAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCAC                                                                                                                   >  SRR19973067.486198/1‑150 (MQ=60)
                                     ATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTA                                                                                                                 >  SRR19973067.2279556/1‑150 (MQ=60)
                                     ATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTA                                                                                                                 >  SRR19973067.2279542/1‑150 (MQ=60)
                                      TAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAA                                                                                                                >  SRR19973067.2630937/1‑150 (MQ=60)
                                      TAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAA                                                                                                                <  SRR19973067.11964/150‑1 (MQ=60)
                                       AAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAA                                                                                                               >  SRR19973067.895240/1‑150 (MQ=60)
                                       AAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAA                                                                                                               >  SRR19973067.282846/1‑150 (MQ=60)
                                        AAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTAGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAG                                                                                                              <  SRR19973067.2426883/150‑1 (MQ=60)
                                            TATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGA                                                                                                          >  SRR19973067.896877/1‑150 (MQ=60)
                                            TATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGA                                                                                                          >  SRR19973067.896880/1‑150 (MQ=60)
                                             ATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGTTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGTCAAACTGAATGAAGTCACTAAAGAAGAC                                                                                                         <  SRR19973067.1633129/150‑1 (MQ=60)
                                             ATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGAC                                                                                                         >  SRR19973067.1231613/1‑150 (MQ=60)
                                             ATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGAC                                                                                                         <  SRR19973067.1633234/150‑1 (MQ=60)
                                             ATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGAC                                                                                                         >  SRR19973067.1532244/1‑150 (MQ=60)
                                              TTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACC                                                                                                        <  SRR19973067.619690/150‑1 (MQ=60)
                                               TGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCT                                                                                                       <  SRR19973067.1289585/150‑1 (MQ=60)
                                               TGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCT                                                                                                       <  SRR19973067.1289718/150‑1 (MQ=60)
                                               TGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCT                                                                                                       <  SRR19973067.1524508/150‑1 (MQ=60)
                                                 CTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGA                                                                                                     >  SRR19973067.1739318/1‑150 (MQ=60)
                                                 CTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGA                                                                                                     >  SRR19973067.1739316/1‑150 (MQ=60)
                                                 CTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGA                                                                                                     <  SRR19973067.297029/150‑1 (MQ=60)
                                                 CTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGA                                                                                                     >  SRR19973067.3235568/1‑150 (MQ=60)
                                                   TATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATT                                                                                                   >  SRR19973067.465531/1‑150 (MQ=60)
                                                        CTACCCACTAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCCGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACT                                                                                              <  SRR19973067.3077209/150‑1 (MQ=60)
                                                         TACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG                                                                                             >  SRR19973067.119993/1‑150 (MQ=60)
                                                         TACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG                                                                                             >  SRR19973067.3181610/1‑150 (MQ=60)
                                                         TACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG                                                                                             >  SRR19973067.3182726/1‑150 (MQ=60)
                                                         TACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG                                                                                             <  SRR19973067.1481642/150‑1 (MQ=60)
                                                           CCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGT                                                                                           <  SRR19973067.2692262/150‑1 (MQ=60)
                                                                   GTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTG                                                                                   >  SRR19973067.1181011/1‑150 (MQ=60)
                                                                   GTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTG                                                                                   <  SRR19973067.2914682/150‑1 (MQ=60)
                                                                    TGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGA                                                                                  >  SRR19973067.1172311/1‑150 (MQ=60)
                                                                     GTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGAT                                                                                 <  SRR19973067.1260052/150‑1 (MQ=60)
                                                                     GTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGAT                                                                                 <  SRR19973067.941916/150‑1 (MQ=60)
                                                                     GTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGAT                                                                                 <  SRR19973067.2729928/150‑1 (MQ=60)
                                                                     GTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGAT                                                                                 <  SRR19973067.1504375/150‑1 (MQ=60)
                                                                       ACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAA                                                                               <  SRR19973067.3258448/150‑1 (MQ=60)
                                                                       ACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAA                                                                               <  SRR19973067.3491686/150‑1 (MQ=60)
                                                                         TTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAAC                                                                              >  SRR19973067.770519/1‑150 (MQ=60)
                                                                         TTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACA                                                                             <  SRR19973067.3535786/150‑1 (MQ=60)
                                                                          TACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAA                                                                            <  SRR19973067.262449/150‑1 (MQ=60)
                                                                             ACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTNAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGA                                                                         <  SRR19973067.2856168/150‑1 (MQ=60)
                                                                              CAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAA                                                                        >  SRR19973067.466750/1‑150 (MQ=60)
                                                                              CAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAA                                                                        <  SRR19973067.1903012/150‑1 (MQ=60)
                                                                                AACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATA                                                                      >  SRR19973067.1625474/1‑150 (MQ=60)
                                                                                  CCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATC                                                                    <  SRR19973067.2383658/150‑1 (MQ=60)
                                                                                  CCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATC                                                                    <  SRR19973067.3150183/150‑1 (MQ=60)
                                                                                      ACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTA                                                                <  SRR19973067.3063716/150‑1 (MQ=60)
                                                                                          GCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTAC                                                            >  SRR19973067.3378846/1‑150 (MQ=60)
                                                                                            CGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAA                                                          >  SRR19973067.1486734/1‑150 (MQ=60)
                                                                                            CGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAA                                                          >  SRR19973067.1481566/1‑150 (MQ=60)
                                                                                            CGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAA                                                          >  SRR19973067.1481472/1‑150 (MQ=60)
                                                                                              CCGCAGCCCACCAGCCTTGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAG                                                        <  SRR19973067.1644461/150‑1 (MQ=60)
                                                                                              CCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAG                                                        <  SRR19973067.10468/150‑1 (MQ=60)
                                                                                               CGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGC                                                       <  SRR19973067.3395474/150‑1 (MQ=60)
                                                                                                GCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCG                                                      <  SRR19973067.1435327/150‑1 (MQ=60)
                                                                                                 CAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGA                                                     >  SRR19973067.1519968/1‑150 (MQ=60)
                                                                                                  AGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGAT                                                    >  SRR19973067.817408/1‑150 (MQ=60)
                                                                                                  AGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGAT                                                    >  SRR19973067.1486290/1‑150 (MQ=60)
                                                                                                   GCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATT                                                   <  SRR19973067.427378/150‑1 (MQ=60)
                                                                                                      CACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCG                                                <  SRR19973067.1785821/150‑1 (MQ=60)
                                                                                                            CCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGAT                                          <  SRR19973067.1116045/150‑1 (MQ=60)
                                                                                                                GTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTG                                      >  SRR19973067.1932159/1‑150 (MQ=60)
                                                                                                                GTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTG                                      >  SRR19973067.1932380/1‑150 (MQ=60)
                                                                                                                GTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTG                                      >  SRR19973067.1065807/1‑150 (MQ=60)
                                                                                                                GTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTG                                      >  SRR19973067.3147833/1‑150 (MQ=60)
                                                                                                                GTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTG                                      <  SRR19973067.2857749/150‑1 (MQ=60)
                                                                                                                GTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTG                                      <  SRR19973067.380008/150‑1 (MQ=60)
                                                                                                                GTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTG                                      <  SRR19973067.1072240/150‑1 (MQ=60)
                                                                                                                  ATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCC                                    >  SRR19973067.2331354/1‑150 (MQ=60)
                                                                                                                  ATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCC                                    >  SRR19973067.638086/1‑150 (MQ=60)
                                                                                                                  ATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCC                                    >  SRR19973067.3530783/1‑150 (MQ=60)
                                                                                                                  ATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCC                                    >  SRR19973067.2331622/1‑150 (MQ=60)
                                                                                                                    TATTAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTT                                  >  SRR19973067.53969/1‑150 (MQ=60)
                                                                                                                    TATTAGTGATATTGGCATCGGGACA‑TGTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTT                                  <  SRR19973067.3238931/150‑1 (MQ=60)
                                                                                                                       TAGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCAT                               <  SRR19973067.364006/150‑1 (MQ=60)
                                                                                                                        AGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATT                              >  SRR19973067.2981458/1‑150 (MQ=60)
                                                                                                                        AGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATT                              >  SRR19973067.2984204/1‑150 (MQ=60)
                                                                                                                        AGTGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATT                              >  SRR19973067.3018902/1‑150 (MQ=60)
                                                                                                                        AGAGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAACGCGGCAACCTGAATGAACTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATT                              <  SRR19973067.1924493/150‑1 (MQ=60)
                                                                                                                          TGATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCG                            <  SRR19973067.548654/150‑1 (MQ=60)
                                                                                                                            ATATTTGCATCGGGACA‑TTTGTCGAACCACGCCAGCTAGGCGGCAAACTGAATGAAGTCACTATAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATTTGTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCG                          <  SRR19973067.2293553/150‑1 (MQ=60)
                                                                                                                            ATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCG                          <  SRR19973067.1606310/150‑1 (MQ=60)
                                                                                                                            ATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCG                          <  SRR19973067.2293616/150‑1 (MQ=60)
                                                                                                                            ATATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCG                          <  SRR19973067.1606005/150‑1 (MQ=60)
                                                                                                                              ATTGGCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCT                        <  SRR19973067.1370762/150‑1 (MQ=60)
                                                                                                                                  GCATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCA                    <  SRR19973067.1974153/150‑1 (MQ=60)
                                                                                                                                    ATCGGGACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACC                  >  SRR19973067.625602/1‑150 (MQ=60)
                                                                                                                                          ACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGAC            >  SRR19973067.699215/1‑150 (MQ=60)
                                                                                                                                          ACA‑TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGAC            >  SRR19973067.698935/1‑150 (MQ=60)
                                                                                                                                                   CGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGG    <  SRR19973067.3310221/150‑1 (MQ=60)
                                                                                                                                                     AACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCT  >  SRR19973067.3371477/1‑150 (MQ=60)
                                                                                                                                                     AACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCT  >  SRR19973067.1451824/1‑150 (MQ=60)
                                                                                                                                                      |                                                                                                                                                    
GGACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACA‑TTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCT  >  CP009273/1770132‑1770429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑