Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F78 I0 R1 626 241.3 10389380 77.7% 8072548 149.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,770,281 T→A 89.4% D146E (GAT→GAA)  ydiF → putative acetyl‑CoA:acetoacetyl‑CoA transferase: alpha subunit/beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,770,2810TA89.4% 840.0 / 71.4 254D146E (GAT→GAA) ydiFputative acetyl‑CoA:acetoacetyl‑CoA transferase: alpha subunit/beta subunit
Reads supporting (aligned to +/- strand):  ref base T (13/14);  new base A (116/111);  total (129/125)
Fisher's exact test for biased strand distribution p-value = 8.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 255 total aligned reads displayed.

ACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTA  >  CP009273/1770134‑1770430
                                                                                                                                                   |                                                                                                                                                     
aCAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAAcc                                                                                                                                                     >  1:2740714/1‑150 (MQ=255)
 cAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTCTTAGTGATATTGGCATCGGGACAGTTGTCGATCCa                                                                                                                                                    >  2:4526011/1‑150 (MQ=255)
 cAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCa                                                                                                                                                    >  2:1244888/1‑150 (MQ=255)
 cAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCa                                                                                                                                                    >  2:3532171/1‑150 (MQ=255)
 cAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCa                                                                                                                                                    <  1:2887889/150‑1 (MQ=255)
 cAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCa                                                                                                                                                    >  1:2834050/1‑150 (MQ=255)
   aTCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACg                                                                                                                                                  <  1:1151318/150‑1 (MQ=255)
         gcgTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCa                                                                                                                                            >  1:1934400/1‑150 (MQ=255)
             aTTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAggc                                                                                                                                        <  1:2928177/150‑1 (MQ=255)
             aTTTCTGAACTCGCAGAACAAAATAAAATGATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAggc                                                                                                                                        >  2:1973031/1‑150 (MQ=255)
                tCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAggcggc                                                                                                                                     <  1:4120630/150‑1 (MQ=255)
                 cTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCa                                                                                                                                    >  2:57710/1‑150 (MQ=255)
                     aCTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACt                                                                                                                                >  2:674282/1‑150 (MQ=255)
                       tCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACtga                                                                                                                              <  1:2103098/150‑1 (MQ=255)
                        cGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACtgaa                                                                                                                             <  2:4857987/150‑1 (MQ=255)
                         gCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACtgaat                                                                                                                            <  1:1451741/150‑1 (MQ=255)
                         gCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACtgaat                                                                                                                            <  1:931344/150‑1 (MQ=255)
                         gCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACtgaat                                                                                                                            >  1:2979363/1‑150 (MQ=255)
                          cAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACtgaatg                                                                                                                           >  2:3117480/1‑150 (MQ=255)
                           aGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACtgaatga                                                                                                                          >  1:2811738/1‑150 (MQ=255)
                           aGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACtgaatga                                                                                                                          >  1:1694402/1‑150 (MQ=255)
                           aGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTAGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACtgaatga                                                                                                                          >  1:1476525/1‑150 (MQ=255)
                             aaCAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAg                                                                                                                        >  2:2196368/1‑150 (MQ=255)
                              aCAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGt                                                                                                                       >  2:859093/1‑150 (MQ=255)
                               cAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTc                                                                                                                      <  1:1429949/150‑1 (MQ=255)
                               cAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTc                                                                                                                      >  1:1042123/1‑150 (MQ=255)
                               cAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTc                                                                                                                      <  1:2550771/150‑1 (MQ=255)
                               cAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTc                                                                                                                      <  2:1893574/150‑1 (MQ=255)
                                 aaataaaatTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCAc                                                                                                                    >  2:2400035/1‑150 (MQ=255)
                                 aaataaaatTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCAc                                                                                                                    <  2:4122501/150‑1 (MQ=255)
                                    taaaatTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTaa                                                                                                                 >  1:3431000/1‑150 (MQ=255)
                                    taaaatTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTaa                                                                                                                 >  2:623844/1‑150 (MQ=255)
                                    taaaatTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTaa                                                                                                                 >  2:3788873/1‑150 (MQ=255)
                                      aaatTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAaag                                                                                                               <  1:4589354/150‑1 (MQ=255)
                                       aatTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAaaga                                                                                                              <  2:1454355/150‑1 (MQ=255)
                                           attGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGAc                                                                                                          >  2:2128235/1‑150 (MQ=255)
                                             tGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCt                                                                                                        <  2:2990147/150‑1 (MQ=255)
                                             tGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCt                                                                                                        >  1:333677/1‑150 (MQ=255)
                                                   taACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTaa                                                                                                  <  1:1439188/150‑1 (MQ=255)
                                                    aaCTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTaaa                                                                                                 >  2:4265531/1‑150 (MQ=255)
                                                    aaCTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTaaa                                                                                                 <  2:4980236/150‑1 (MQ=255)
                                                     aCTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAAc                                                                                                >  1:3141939/1‑150 (MQ=255)
                                                        aCCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTgg                                                                                             <  2:2184928/150‑1 (MQ=255)
                                                         cccACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGt                                                                                            <  2:1877316/150‑1 (MQ=255)
                                                         cccACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGt                                                                                            <  2:1877408/150‑1 (MQ=255)
                                                          ccACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTc                                                                                           <  2:3810286/150‑1 (MQ=255)
                                                          ccACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTc                                                                                           <  2:2311872/150‑1 (MQ=255)
                                                            acaAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGa                                                                                         >  2:146114/1‑150 (MQ=255)
                                                              aaGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGt                                                                                       <  2:980920/150‑1 (MQ=255)
                                                              aaGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGt                                                                                       >  1:4368669/1‑150 (MQ=255)
                                                                ggTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGttt                                                                                     >  2:276558/1‑150 (MQ=255)
                                                                   gtACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATAATGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGAt                                                                                  >  2:3933398/1‑150 (MQ=255)
                                                                        tccacaAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACGTTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTCAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACaa                                                                             >  2:1794412/3‑150 (MQ=255)
                                                                           acaAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTCAACTGGTCGAGTTTGATAACACAGa                                                                          >  2:4491446/1‑150 (MQ=255)
                                                                           acaAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGa                                                                          >  1:586390/1‑150 (MQ=255)
                                                                              aaCCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAata                                                                       <  2:385243/150‑1 (MQ=255)
                                                                              aaCCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAata                                                                       >  1:3175198/1‑150 (MQ=255)
                                                                                ccTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATAtc                                                                     <  1:3130327/150‑1 (MQ=255)
                                                                                ccTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATAtc                                                                     >  1:2387045/1‑150 (MQ=255)
                                                                                 cTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATAtct                                                                    >  2:5003281/1‑150 (MQ=255)
                                                                                 cTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATAtct                                                                    >  2:5017845/1‑150 (MQ=255)
                                                                                     cgcgCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTAt                                                                <  2:4756182/150‑1 (MQ=255)
                                                                                             cgcAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGc                                                        <  2:1167854/150‑1 (MQ=255)
                                                                                                aGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGAt                                                     >  1:3736143/1‑150 (MQ=255)
                                                                                                aGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGAt                                                     <  2:1045154/150‑1 (MQ=255)
                                                                                                 gCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGAtt                                                    <  2:3744727/150‑1 (MQ=255)
                                                                                                  cccACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTg                                                   <  1:4718655/150‑1 (MQ=255)
                                                                                                          ccTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGat                                           <  1:3343364/150‑1 (MQ=255)
                                                                                                          ccTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGat                                           <  2:3077440/150‑1 (MQ=255)
                                                                                                           cTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGata                                          <  1:477703/150‑1 (MQ=255)
                                                                                                            tGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGatat                                         >  1:4165557/1‑150 (MQ=255)
                                                                                                            tGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGatat                                         >  2:5149350/1‑150 (MQ=255)
                                                                                                            tGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGatat                                         >  1:1570413/1‑150 (MQ=255)
                                                                                                             ggTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACTCCATCAAGGCGTCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATAtt                                        <  2:463689/150‑1 (MQ=255)
                                                                                                             ggTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATAtt                                        <  1:4688529/150‑1 (MQ=255)
                                                                                                               tattatTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGc                                      >  1:733917/1‑150 (MQ=255)
                                                                                                               tattatTAGTGAGATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGc                                      <  2:2738134/150‑1 (MQ=255)
                                                                                                                   attaGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTc                                  <  1:4883676/150‑1 (MQ=255)
                                                                                                                    ttaGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCa                                 <  1:1384671/150‑1 (MQ=255)
                                                                                                                      aGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCAtt                               >  1:131401/1‑150 (MQ=255)
                                                                                                                       gTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTc                              <  1:1573275/150‑1 (MQ=255)
                                                                                                                          atatTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTcgcg                           <  1:1763133/150‑1 (MQ=255)
                                                                                                                            atTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCt                         <  1:2226480/150‑1 (MQ=255)
                                                                                                                            atTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCt                         >  2:3557128/1‑150 (MQ=255)
                                                                                                                            atTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCt                         <  1:3876118/150‑1 (MQ=255)
                                                                                                                            atTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCt                         <  2:297376/150‑1 (MQ=255)
                                                                                                                            atTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCt                         >  2:2878618/1‑150 (MQ=255)
                                                                                                                            atTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCt                         >  1:4334154/1‑150 (MQ=255)
                                                                                                                             ttGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTa                        >  2:1815067/1‑150 (MQ=255)
                                                                                                                              tGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTac                       >  2:3121699/1‑150 (MQ=255)
                                                                                                                                 cATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTaccac                    >  1:2293209/1‑150 (MQ=255)
                                                                                                                                  aTCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTaccacc                   <  1:3834646/150‑1 (MQ=255)
                                                                                                                                    cGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTg                 <  2:4472078/150‑1 (MQ=255)
                                                                                                                                          aTTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAg           >  1:3757015/1‑150 (MQ=255)
                                                                                                                                              gTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGaa       >  1:2621015/1‑150 (MQ=255)
                                                                                                                                              gTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGaa       >  2:4365084/1‑150 (MQ=255)
                                                                                                                                              gTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGaa       <  2:4234835/150‑1 (MQ=255)
                                                                                                                                               tCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAg      >  1:5132425/1‑150 (MQ=255)
                                                                                                                                               tCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAg      >  2:759883/1‑150 (MQ=255)
                                                                                                                                                cGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAgg     >  2:2931060/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
ACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTA  >  CP009273/1770134‑1770430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTA  >  CP009273/1770132‑1770430
                                                                                                                                                     |                                                                                                                                                     
GGACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAA                                                                                                                                                       <  SRR19973083.1061637/150‑1 (MQ=60)
GGACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAA                                                                                                                                                       >  SRR19973083.583535/1‑150 (MQ=60)
GGACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAA                                                                                                                                                       <  SRR19973083.2705869/150‑1 (MQ=60)
GGACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAA                                                                                                                                                       <  SRR19973083.2705926/150‑1 (MQ=60)
  ACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACC                                                                                                                                                     >  SRR19973083.2754380/1‑150 (MQ=60)
   CAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCA                                                                                                                                                    >  SRR19973083.2848071/1‑150 (MQ=60)
   CAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCA                                                                                                                                                    >  SRR19973083.1710662/1‑150 (MQ=60)
   CAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCA                                                                                                                                                    <  SRR19973083.2902054/150‑1 (MQ=60)
   CAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCA                                                                                                                                                    <  SRR19973083.2902043/150‑1 (MQ=60)
     ATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACG                                                                                                                                                  <  SRR19973083.1157058/150‑1 (MQ=60)
     ATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACG                                                                                                                                                  <  SRR19973083.1453492/150‑1 (MQ=60)
          CGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGC                                                                                                                                             >  SRR19973083.1754734/1‑150 (MQ=60)
           GCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCA                                                                                                                                            <  SRR19973083.4314900/150‑1 (MQ=60)
           GCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCA                                                                                                                                            >  SRR19973083.1944267/1‑150 (MQ=60)
           GCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCA                                                                                                                                            >  SRR19973083.3792663/1‑150 (MQ=60)
           GCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCA                                                                                                                                            >  SRR19973083.4648273/1‑150 (MQ=60)
           GCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCA                                                                                                                                            >  SRR19973083.3792929/1‑150 (MQ=60)
            CGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAAACACGCCAGCAA                                                                                                                                           <  SRR19973083.2731091/150‑1 (MQ=60)
               ATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGC                                                                                                                                        <  SRR19973083.2941916/150‑1 (MQ=60)
               ATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGC                                                                                                                                        <  SRR19973083.2942489/150‑1 (MQ=60)
                  TCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGC                                                                                                                                     <  SRR19973083.4138864/150‑1 (MQ=60)
                        CTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTG                                                                                                                               <  SRR19973083.2541078/150‑1 (MQ=60)
                        CTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTG                                                                                                                               >  SRR19973083.5018555/1‑150 (MQ=60)
                         TCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGA                                                                                                                              <  SRR19973083.2113745/150‑1 (MQ=60)
                           GCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAAT                                                                                                                            <  SRR19973083.1459053/150‑1 (MQ=60)
                           GCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAAT                                                                                                                            <  SRR19973083.2449027/150‑1 (MQ=60)
                           GCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAAT                                                                                                                            <  SRR19973083.935867/150‑1 (MQ=60)
                           GCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAAT                                                                                                                            >  SRR19973083.2993807/1‑150 (MQ=60)
                           GCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAAT                                                                                                                            <  SRR19973083.4156107/150‑1 (MQ=60)
                             AGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGA                                                                                                                          >  SRR19973083.2825673/1‑150 (MQ=60)
                             AGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGA                                                                                                                          >  SRR19973083.1483963/1‑150 (MQ=60)
                             AGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGA                                                                                                                          >  SRR19973083.1702761/1‑150 (MQ=60)
                             AGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTAGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGA                                                                                                                          >  SRR19973083.1483970/1‑150 (MQ=60)
                                 CAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTC                                                                                                                      <  SRR19973083.1437115/150‑1 (MQ=60)
                                 CAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTC                                                                                                                      >  SRR19973083.1050408/1‑150 (MQ=60)
                                 CAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTC                                                                                                                      >  SRR19973083.1047360/1‑150 (MQ=60)
                                 CAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTC                                                                                                                      <  SRR19973083.2563678/150‑1 (MQ=60)
                                   AAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCAC                                                                                                                    >  SRR19973083.2345973/1‑150 (MQ=60)
                                      TAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAA                                                                                                                 >  SRR19973083.3446786/1‑150 (MQ=60)
                                        AAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAG                                                                                                               >  SRR19973083.4377156/1‑150 (MQ=60)
                                        AAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAG                                                                                                               <  SRR19973083.4609182/150‑1 (MQ=60)
                                           TTATTGCTTATAACTACCCACATGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAG                                                                                                            <  SRR19973083.28403/150‑1 (MQ=60)
                                           TTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAG                                                                                                            <  SRR19973083.28325/150‑1 (MQ=60)
                                            TATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGA                                                                                                           <  SRR19973083.5052429/150‑1 (MQ=60)
                                               TGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCT                                                                                                        >  SRR19973083.335317/1‑150 (MQ=60)
                                               TGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCT                                                                                                        >  SRR19973083.335352/1‑150 (MQ=60)
                                               TGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCT                                                                                                        >  SRR19973083.335306/1‑150 (MQ=60)
                                                   TATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATT                                                                                                    >  SRR19973083.4481471/1‑150 (MQ=60)
                                                     TAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAA                                                                                                  <  SRR19973083.1446419/150‑1 (MQ=60)
                                                     TAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAA                                                                                                  <  SRR19973083.1446323/150‑1 (MQ=60)
                                                       ACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAAC                                                                                                >  SRR19973083.3156961/1‑150 (MQ=60)
                                                       ACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAAC                                                                                                <  SRR19973083.65119/150‑1 (MQ=60)
                                                           CCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGT                                                                                            >  SRR19973083.2894389/1‑150 (MQ=60)
                                                           CCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGT                                                                                            >  SRR19973083.394612/1‑150 (MQ=60)
                                                            CCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTC                                                                                           <  SRR19973083.3880700/150‑1 (MQ=60)
                                                                AAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGT                                                                                       >  SRR19973083.4573260/1‑150 (MQ=60)
                                                                AAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGT                                                                                       >  SRR19973083.4387642/1‑150 (MQ=60)
                                                                     GTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGAT                                                                                  >  SRR19973083.3156255/1‑150 (MQ=60)
                                                                     GTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGAT                                                                                  >  SRR19973083.3156219/1‑150 (MQ=60)
                                                                     GTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGAT                                                                                  >  SRR19973083.2961717/1‑150 (MQ=60)
                                                                        CTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAAC                                                                               >  SRR19973083.1409274/1‑150 (MQ=60)
                                                                            CACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAG                                                                           >  SRR19973083.3400676/1‑150 (MQ=60)
                                                                            CACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAG                                                                           >  SRR19973083.3068744/1‑150 (MQ=60)
                                                                             ACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGA                                                                          >  SRR19973083.589330/1‑150 (MQ=60)
                                                                              CAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAA                                                                         <  SRR19973083.4672715/150‑1 (MQ=60)
                                                                                AACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATA                                                                       <  SRR19973083.860224/150‑1 (MQ=60)
                                                                                AACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATA                                                                       >  SRR19973083.3190330/1‑150 (MQ=60)
                                                                                AACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATA                                                                       >  SRR19973083.3190612/1‑150 (MQ=60)
                                                                                  CCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATC                                                                     <  SRR19973083.3145293/150‑1 (MQ=60)
                                                                                  CCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATC                                                                     >  SRR19973083.2399325/1‑150 (MQ=60)
                                                                                    TTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTC                                                                   <  SRR19973083.1229196/150‑1 (MQ=60)
                                                                                      ACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTA                                                                 <  SRR19973083.556374/150‑1 (MQ=60)
                                                                                        GCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATT                                                               <  SRR19973083.3767029/150‑1 (MQ=60)
                                                                                        GCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATT                                                               >  SRR19973083.2666478/1‑150 (MQ=60)
                                                                                            CGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAA                                                           >  SRR19973083.2164026/1‑150 (MQ=60)
                                                                                                  AGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGAT                                                     >  SRR19973083.3753097/1‑150 (MQ=60)
                                                                                                    CCCACCAGCCTGGTGTTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTG                                                   <  SRR19973083.4738892/150‑1 (MQ=60)
                                                                                                    CCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTG                                                   <  SRR19973083.4738836/150‑1 (MQ=60)
                                                                                                    CCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTG                                                   <  SRR19973083.4738834/150‑1 (MQ=60)
                                                                                                    CCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGAGATTG                                                   >  SRR19973083.3580397/1‑150 (MQ=60)
                                                                                                            CCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGAT                                           <  SRR19973083.3358968/150‑1 (MQ=60)
                                                                                                             CTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATA                                          <  SRR19973083.479970/150‑1 (MQ=60)
                                                                                                              TGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATAT                                         >  SRR19973083.4183924/1‑150 (MQ=60)
                                                                                                              TGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATAT                                         >  SRR19973083.1578299/1‑150 (MQ=60)
                                                                                                               GGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATT                                        >  SRR19973083.1429079/1‑150 (MQ=60)
                                                                                                               GGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATT                                        <  SRR19973083.476816/150‑1 (MQ=60)
                                                                                                               GGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATT                                        <  SRR19973083.2113323/150‑1 (MQ=60)
                                                                                                               GGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATT                                        <  SRR19973083.4708645/150‑1 (MQ=60)
                                                                                                                GTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTG                                       >  SRR19973083.391043/1‑150 (MQ=60)
                                                                                                                 TATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGC                                      <  SRR19973083.3548383/150‑1 (MQ=60)
                                                                                                                 TATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGC                                      >  SRR19973083.737524/1‑150 (MQ=60)
                                                                                                                  ATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCC                                     >  SRR19973083.991027/1‑150 (MQ=60)
                                                                                                                  ATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCC                                     >  SRR19973083.3364305/1‑150 (MQ=60)
                                                                                                                   TTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCT                                    <  SRR19973083.1569931/150‑1 (MQ=60)
                                                                                                                   TTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCT                                    <  SRR19973083.1364545/150‑1 (MQ=60)
                                                                                                                     ATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTC                                  <  SRR19973083.4904652/150‑1 (MQ=60)
                                                                                                                     ATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTC                                  <  SRR19973083.2969551/150‑1 (MQ=60)
                                                                                                                      TTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCA                                 <  SRR19973083.1391567/150‑1 (MQ=60)
                                                                                                                      TTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCA                                 <  SRR19973083.201595/150‑1 (MQ=60)
                                                                                                                        AGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATT                               >  SRR19973083.132232/1‑150 (MQ=60)
                                                                                                                        AGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGAGATTGCCTTCATT                               >  SRR19973083.2760803/1‑150 (MQ=60)
                                                                                                                         GTGATATTGTCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTC                              <  SRR19973083.1874702/150‑1 (MQ=60)
                                                                                                                         GTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTC                              <  SRR19973083.679488/150‑1 (MQ=60)
                                                                                                                         GTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTC                              <  SRR19973083.1581112/150‑1 (MQ=60)
                                                                                                                         GTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTC                              <  SRR19973083.1581173/150‑1 (MQ=60)
                                                                                                                         GTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTC                              <  SRR19973083.1581238/150‑1 (MQ=60)
                                                                                                                            ATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCG                           <  SRR19973083.1771843/150‑1 (MQ=60)
                                                                                                                              ATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCT                         >  SRR19973083.4352952/1‑150 (MQ=60)
                                                                                                                              ATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCT                         <  SRR19973083.3893424/150‑1 (MQ=60)
                                                                                                                              ATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCT                         <  SRR19973083.2237753/150‑1 (MQ=60)
                                                                                                                                   CATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCAC                    >  SRR19973083.2304957/1‑150 (MQ=60)
                                                                                                                                   CATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCAC                    >  SRR19973083.4896604/1‑150 (MQ=60)
                                                                                                                                    ATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACC                   <  SRR19973083.1244373/150‑1 (MQ=60)
                                                                                                                                    ATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACC                   >  SRR19973083.1622208/1‑150 (MQ=60)
                                                                                                                                    ATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACC                   <  SRR19973083.3851856/150‑1 (MQ=60)
                                                                                                                                          ACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGAC             <  SRR19973083.3179418/150‑1 (MQ=60)
                                                                                                                                            ATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAG           >  SRR19973083.3774034/1‑150 (MQ=60)
                                                                                                                                              TTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTG         >  SRR19973083.1524461/1‑150 (MQ=60)
                                                                                                                                               TGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGA        >  SRR19973083.2595106/1‑150 (MQ=60)
                                                                                                                                                GTCGAACCACGCCAGCAAGGCGTCAAACTGAATTAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAA       <  SRR19973083.1456272/150‑1 (MQ=60)
                                                                                                                                                GTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAA       <  SRR19973083.2254288/150‑1 (MQ=60)
                                                                                                                                                GTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAA       >  SRR19973083.2634175/1‑150 (MQ=60)
                                                                                                                                                 TCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAG      >  SRR19973083.5154262/1‑150 (MQ=60)
                                                                                                                                                  CGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGG     >  SRR19973083.4211822/1‑150 (MQ=60)
                                                                                                                                                   GAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGC    >  SRR19973083.570194/1‑150 (MQ=60)
                                                                                                                                                     TCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTA  <  SRR19973083.2291944/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
GGACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTA  >  CP009273/1770132‑1770430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑