Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,770,281 T→A 100% D146E (GAT→GAA)  ydiF → putative acetyl‑CoA:acetoacetyl‑CoA transferase: alpha subunit/beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,770,2810TA99.6% 920.2 / ‑4.4 254D146E (GAT→GAA) ydiFputative acetyl‑CoA:acetoacetyl‑CoA transferase: alpha subunit/beta subunit
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base A (141/112);  total (142/112)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 254 total aligned reads displayed.

ACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGG  >  CP009273/1770134‑1770427
                                                                                                                                                   |                                                                                                                                                  
aCAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAAcc                                                                                                                                                  <  1:521173/150‑1 (MQ=255)
 cAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCa                                                                                                                                                 >  1:2048817/1‑150 (MQ=255)
  aaTCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCAc                                                                                                                                                >  1:703633/1‑150 (MQ=255)
  aaTCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCAc                                                                                                                                                >  2:3943119/1‑150 (MQ=255)
  aaTCGCCGCGTATGTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCAc                                                                                                                                                <  2:1559464/150‑1 (MQ=255)
       ccgcGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCGTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAg                                                                                                                                           <  2:2937985/150‑1 (MQ=255)
        cgcgTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGc                                                                                                                                          >  1:980058/1‑150 (MQ=255)
         gcgTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCa                                                                                                                                         <  1:3113915/150‑1 (MQ=255)
         gcgTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCa                                                                                                                                         <  2:154482/150‑1 (MQ=255)
         gcgTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCa                                                                                                                                         <  1:186845/150‑1 (MQ=255)
              tttCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAggcg                                                                                                                                    <  2:2373419/150‑1 (MQ=255)
                  tGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCaa                                                                                                                                >  1:1174364/1‑150 (MQ=255)
                           aGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACtgaatga                                                                                                                       >  2:690286/1‑150 (MQ=255)
                            gAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACtgaatgaa                                                                                                                      >  2:2208768/1‑150 (MQ=255)
                             aaCAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAg                                                                                                                     >  2:2054780/1‑150 (MQ=255)
                              aCAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGt                                                                                                                    <  1:3197198/150‑1 (MQ=255)
                                 aaataaaatTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCAc                                                                                                                 >  2:1215401/1‑150 (MQ=255)
                                  aataaaatTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACt                                                                                                                >  1:2524900/1‑150 (MQ=255)
                                    taaaatTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTaa                                                                                                              >  1:2203151/1‑150 (MQ=255)
                                     aaaatTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTaaa                                                                                                             <  1:1205499/150‑1 (MQ=255)
                                        attattGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAaagaa                                                                                                          <  1:907855/150‑1 (MQ=255)
                                         ttattGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAaagaag                                                                                                         >  2:2460299/1‑150 (MQ=255)
                                          tattGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAagaaga                                                                                                        >  2:483070/1‑150 (MQ=255)
                                              gCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTg                                                                                                    >  1:4113241/1‑150 (MQ=255)
                                               cTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGa                                                                                                   <  2:1087719/150‑1 (MQ=255)
                                               cTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGa                                                                                                   >  1:1363690/1‑150 (MQ=255)
                                                   taACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTaa                                                                                               <  1:2614020/150‑1 (MQ=255)
                                                    aaCTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTaaa                                                                                              >  1:2147770/1‑150 (MQ=255)
                                                     aCTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAAc                                                                                             >  2:217535/1‑150 (MQ=255)
                                                     aCTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAAc                                                                                             >  2:1402873/1‑150 (MQ=255)
                                                     aCTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAAc                                                                                             >  2:1451054/1‑150 (MQ=255)
                                                     aCTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAAc                                                                                             >  1:100926/1‑150 (MQ=255)
                                                       tACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTg                                                                                           >  2:1079121/1‑150 (MQ=255)
                                                       tACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTg                                                                                           >  1:3215913/1‑150 (MQ=255)
                                                       tACCCACAAGGTGGAATTACACAAACCTTACGCGCCGCCGCATCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTg                                                                                           >  1:1003307/1‑150 (MQ=255)
                                                        aCCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTgg                                                                                          >  2:4070921/1‑150 (MQ=255)
                                                         cccACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGt                                                                                         >  2:2844656/1‑150 (MQ=255)
                                                          ccACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCACGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTc                                                                                        <  2:2998879/150‑1 (MQ=255)
                                                          ccACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTc                                                                                        <  2:78891/150‑1 (MQ=255)
                                                           cacaAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCg                                                                                       <  2:1878245/150‑1 (MQ=255)
                                                            acaAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTATAGAAGACCTGATTAAACTGGTCGa                                                                                      >  2:272627/1‑150 (MQ=255)
                                                            acaAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGa                                                                                      <  1:333597/150‑1 (MQ=255)
                                                            acaAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGa                                                                                      >  2:3232107/1‑150 (MQ=255)
                                                             caAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAg                                                                                     <  1:3583824/150‑1 (MQ=255)
                                                             caAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAg                                                                                     <  2:2591569/150‑1 (MQ=255)
                                                              aaGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGt                                                                                    >  2:2385881/1‑150 (MQ=255)
                                                               aGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGtt                                                                                   <  1:2217622/150‑1 (MQ=255)
                                                               aGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGtt                                                                                   >  2:604906/1‑150 (MQ=255)
                                                               aGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGtt                                                                                   <  2:3322455/150‑1 (MQ=255)
                                                               aGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGtt                                                                                   <  1:3688661/150‑1 (MQ=255)
                                                                ggTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGttt                                                                                  <  1:1762013/150‑1 (MQ=255)
                                                                   gtACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGAt                                                                               <  2:3089956/150‑1 (MQ=255)
                                                                   gtACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGAt                                                                               <  1:2940770/150‑1 (MQ=255)
                                                                   gtACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGAGATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGAt                                                                               <  2:831846/150‑1 (MQ=255)
                                                                      cTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAAc                                                                            <  2:2087726/150‑1 (MQ=255)
                                                                         acacAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACAGAAAGAAGTCACTAAAGAAGACCTGATTAAACAGGTCGAGTTTGATAACaaa                                                                         >  1:2366269/1‑150 (MQ=255)
                                                                             aaaCCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGAGTAAACTGGTCGAGTTTGATAACAAAGAat                                                                     >  2:3218385/1‑150 (MQ=255)
                                                                               aCCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAatat                                                                   <  2:3363761/150‑1 (MQ=255)
                                                                                  ttACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATAtctc                                                                >  1:3866708/1‑150 (MQ=255)
                                                                                  ttACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATAtctc                                                                <  1:1155679/150‑1 (MQ=255)
                                                                                   tACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATctct                                                               >  2:4124403/1‑150 (MQ=255)
                                                                                    aCGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTa                                                              >  2:1752614/1‑150 (MQ=255)
                                                                                     cgcgCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCTAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTAt                                                             <  2:289251/150‑1 (MQ=255)
                                                                                     cgcgCAGCCGCAGCCCAACAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGAGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTAGATAACCAAGAATATCTCTAt                                                             >  2:1985275/1‑150 (MQ=255)
                                                                                       cgccgccgcAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTa                                                           <  1:3776661/150‑1 (MQ=255)
                                                                                       cgccgccgcAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTa                                                           <  1:4129456/150‑1 (MQ=255)
                                                                                        gccgccgcAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTAc                                                          <  1:3833914/150‑1 (MQ=255)
                                                                                         ccgccgcAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACa                                                         >  2:3756618/1‑150 (MQ=255)
                                                                                             cgcAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGc                                                     >  2:1595778/1‑150 (MQ=255)
                                                                                              gcAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCg                                                    >  2:245392/1‑150 (MQ=255)
                                                                                                aGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGAt                                                  >  2:2125832/1‑150 (MQ=255)
                                                                                                    caccaGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTgct                                              >  1:2416496/1‑149 (MQ=255)
                                                                                                       caGCCTGGTATTATTAGAGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAATACATGATTAAACTGGTCGAGTTTGATAACAAATAATATCTCTATTAAAAAGCGATTGCGCCa                                           >  1:4026617/1‑150 (MQ=255)
                                                                                                        aGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAg                                          <  2:3957401/150‑1 (MQ=255)
                                                                                                        aGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAg                                          >  1:2634471/1‑150 (MQ=255)
                                                                                                         gCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGa                                         >  1:161662/1‑150 (MQ=255)
                                                                                                             ggTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATAtt                                     <  2:733704/150‑1 (MQ=255)
                                                                                                             ggTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATAtt                                     >  1:431949/1‑150 (MQ=255)
                                                                                                               tattatTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGc                                   >  2:2728826/1‑150 (MQ=255)
                                                                                                                 ttattaGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCt                                 >  1:2358154/1‑150 (MQ=255)
                                                                                                                  tattaGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCtt                                <  2:284620/150‑1 (MQ=255)
                                                                                                                   attaGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTc                               >  1:599027/1‑150 (MQ=255)
                                                                                                                    ttaGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCa                              <  2:632737/150‑1 (MQ=255)
                                                                                                                      aGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCAtt                            >  1:293457/1‑150 (MQ=255)
                                                                                                                      aGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCAtt                            >  1:32659/1‑150 (MQ=255)
                                                                                                                        tGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTcg                          >  1:1466486/1‑150 (MQ=255)
                                                                                                                         gATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTcgc                         >  2:1358774/1‑150 (MQ=255)
                                                                                                                         gATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTcgc                         >  2:3452286/1‑150 (MQ=255)
                                                                                                                         gATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTcgc                         >  1:1267283/1‑150 (MQ=255)
                                                                                                                          atatTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTcgcg                        <  1:2250190/150‑1 (MQ=255)
                                                                                                                          atatTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTcgcg                        <  1:2250079/150‑1 (MQ=255)
                                                                                                                            atTGGCATCGGTACATTTGTCGAACCACGCCATCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTTGTCGAGTTTTATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTTCCTTCATACGCGCt                      >  1:1377203/1‑150 (MQ=255)
                                                                                                                            atTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCt                      >  2:1840540/1‑150 (MQ=255)
                                                                                                                              tGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTac                    >  2:3567477/1‑150 (MQ=255)
                                                                                                                                    cGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTg              <  1:1713055/150‑1 (MQ=255)
                                                                                                                                     gggACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGc             >  1:2683706/1‑150 (MQ=255)
                                                                                                                                     gggACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGc             >  1:908086/1‑150 (MQ=255)
                                                                                                                                           tttGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGt       <  1:3120623/150‑1 (MQ=255)
                                                                                                                                           tttGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGt       <  2:2414731/150‑1 (MQ=255)
                                                                                                                                                cGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAgg  <  1:3250457/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGG  >  CP009273/1770134‑1770427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCT  >  CP009273/1770132‑1770429
                                                                                                                                                     |                                                                                                                                                    
GGACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAA                                                                                                                                                      <  SRR19973074.3717541/150‑1 (MQ=60)
GGACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAA                                                                                                                                                      <  SRR19973074.3717547/150‑1 (MQ=60)
  ACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACC                                                                                                                                                    <  SRR19973074.522212/150‑1 (MQ=60)
   CAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCA                                                                                                                                                   >  SRR19973074.2052672/1‑150 (MQ=60)
   CAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCA                                                                                                                                                   >  SRR19973074.2052652/1‑150 (MQ=60)
    AATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCAC                                                                                                                                                  >  SRR19973074.704935/1‑150 (MQ=60)
          CGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGC                                                                                                                                            >  SRR19973074.981894/1‑150 (MQ=60)
           GCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCA                                                                                                                                           <  SRR19973074.187246/150‑1 (MQ=60)
           GCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCA                                                                                                                                           <  SRR19973074.3119628/150‑1 (MQ=60)
           GCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCA                                                                                                                                           <  SRR19973074.3119724/150‑1 (MQ=60)
                TTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCG                                                                                                                                      <  SRR19973074.593028/150‑1 (MQ=60)
                TTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCG                                                                                                                                      >  SRR19973074.418627/1‑150 (MQ=60)
                    TGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAA                                                                                                                                  <  SRR19973074.3623852/150‑1 (MQ=60)
                    TGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAA                                                                                                                                  >  SRR19973074.1176598/1‑150 (MQ=60)
                     GAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAA                                                                                                                                 <  SRR19973074.3175201/150‑1 (MQ=60)
                       ACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACT                                                                                                                               <  SRR19973074.1226059/150‑1 (MQ=60)
                       ACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACT                                                                                                                               <  SRR19973074.2197194/150‑1 (MQ=60)
                         TCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGA                                                                                                                             <  SRR19973074.372243/150‑1 (MQ=60)
                             AGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGA                                                                                                                         >  SRR19973074.1387168/1‑150 (MQ=60)
                              GAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAA                                                                                                                        >  SRR19973074.1760580/1‑150 (MQ=60)
                               AACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACAAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAG                                                                                                                       >  SRR19973074.3208206/1‑150 (MQ=60)
                                ACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGT                                                                                                                      <  SRR19973074.3203092/150‑1 (MQ=60)
                                  AAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCA                                                                                                                    >  SRR19973074.221745/1‑150 (MQ=60)
                                  AAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCA                                                                                                                    >  SRR19973074.2942068/1‑150 (MQ=60)
                                    AATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACT                                                                                                                  >  SRR19973074.2529607/1‑150 (MQ=60)
                                     ATAAAATTAGTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTA                                                                                                                 <  SRR19973074.2246365/150‑1 (MQ=60)
                                      TAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAA                                                                                                                >  SRR19973074.2207296/1‑150 (MQ=60)
                                       AAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCGAACTGAATGAAGTCACTAAA                                                                                                               <  SRR19973074.1207700/150‑1 (MQ=60)
                                       AAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAA                                                                                                               >  SRR19973074.2956810/1‑150 (MQ=60)
                                       AAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAA                                                                                                               >  SRR19973074.2956780/1‑150 (MQ=60)
                                       AAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAA                                                                                                               <  SRR19973074.1207808/150‑1 (MQ=60)
                                         AATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGA                                                                                                             >  SRR19973074.3816099/1‑150 (MQ=60)
                                         AATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGA                                                                                                             >  SRR19973074.4075794/1‑150 (MQ=60)
                                          ATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAA                                                                                                            <  SRR19973074.909582/150‑1 (MQ=60)
                                          ATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAA                                                                                                            <  SRR19973074.909720/150‑1 (MQ=60)
                                          ATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAA                                                                                                            >  SRR19973074.3263386/1‑150 (MQ=60)
                                           TTATTGCTTATAACGACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAG                                                                                                           <  SRR19973074.879902/150‑1 (MQ=60)
                                            TATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGA                                                                                                          <  SRR19973074.2568707/150‑1 (MQ=60)
                                             ATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGAC                                                                                                         <  SRR19973074.1815481/150‑1 (MQ=60)
                                                GCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTG                                                                                                      >  SRR19973074.4120980/1‑150 (MQ=60)
                                                GCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTG                                                                                                      >  SRR19973074.4120834/1‑150 (MQ=60)
                                                 CTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGA                                                                                                     >  SRR19973074.2710295/1‑150 (MQ=60)
                                                 CTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGA                                                                                                     >  SRR19973074.1366285/1‑150 (MQ=60)
                                                 CTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGA                                                                                                     >  SRR19973074.1366214/1‑150 (MQ=60)
                                                     TAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAA                                                                                                 <  SRR19973074.2618911/150‑1 (MQ=60)
                                                      AACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAA                                                                                                >  SRR19973074.2151809/1‑150 (MQ=60)
                                                      AACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAA                                                                                                >  SRR19973074.2151784/1‑150 (MQ=60)
                                                      AACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAA                                                                                                >  SRR19973074.2151810/1‑150 (MQ=60)
                                                      AACTACCCACAAGGTGTACTTACACAAACCNTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAA                                                                                                >  SRR19973074.3864408/1‑150 (MQ=60)
                                                       ACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAAC                                                                                               >  SRR19973074.101175/1‑150 (MQ=60)
                                                       ACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAAC                                                                                               <  SRR19973074.1343205/150‑1 (MQ=60)
                                                       ACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAAC                                                                                               >  SRR19973074.3339485/1‑150 (MQ=60)
                                                         TACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG                                                                                             >  SRR19973074.1005194/1‑150 (MQ=60)
                                                         TACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG                                                                                             >  SRR19973074.3221855/1‑150 (MQ=60)
                                                         TACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG                                                                                             >  SRR19973074.3221861/1‑150 (MQ=60)
                                                         TACCCACAAGGTGGAATTACACAAACCTTACGCGCCGCCGCATCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG                                                                                             >  SRR19973074.1005169/1‑150 (MQ=60)
                                                          ACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGG                                                                                            >  SRR19973074.1315384/1‑150 (MQ=60)
                                                              ACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGA                                                                                        <  SRR19973074.334205/150‑1 (MQ=60)
                                                               CAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAG                                                                                       <  SRR19973074.3590433/150‑1 (MQ=60)
                                                               CAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAG                                                                                       >  SRR19973074.1182569/1‑150 (MQ=60)
                                                               CAAGGTGTACTTACACAAACCTTACGCGCCGCCGAAGCCCACCAGCCAGGTAGAATTAGTGATATTGTCATCCGGACAATTGTCGAACAACGCCACCAAGGCGGCAAACTGAATGAAGACACTAAAGAAGACATTATTAAAATGGTCGAG                                                                                       >  SRR19973074.956234/1‑150 (MQ=60)
                                                                 AGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTT                                                                                     <  SRR19973074.2221797/150‑1 (MQ=60)
                                                                 AGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTT                                                                                     <  SRR19973074.3695481/150‑1 (MQ=60)
                                                                 AGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTT                                                                                     <  SRR19973074.3695482/150‑1 (MQ=60)
                                                                 AGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTT                                                                                     >  SRR19973074.2269326/1‑150 (MQ=60)
                                                                  GGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTT                                                                                    <  SRR19973074.1765311/150‑1 (MQ=60)
                                                                    TGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGA                                                                                  >  SRR19973074.4061857/1‑150 (MQ=60)
                                                                     GTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGAT                                                                                 <  SRR19973074.2946239/150‑1 (MQ=60)
                                                                       ACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAA                                                                               <  SRR19973074.3356286/150‑1 (MQ=60)
                                                                       ACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAA                                                                               <  SRR19973074.248119/150‑1 (MQ=60)
                                                                       ACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAA                                                                               >  SRR19973074.2248826/1‑150 (MQ=60)
                                                                          TACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAA                                                                            >  SRR19973074.1380970/1‑150 (MQ=60)
                                                                           ACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAA                                                                           >  SRR19973074.2370676/1‑150 (MQ=60)
                                                                           ACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAA                                                                           >  SRR19973074.2370649/1‑150 (MQ=60)
                                                                           ACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACAGAAAGAAGTCACTAAAGAAGACCTGATTAAACAGGTCGAGTTTGATAACAAA                                                                           >  SRR19973074.2370672/1‑150 (MQ=60)
                                                                               AAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAAT                                                                       <  SRR19973074.2564126/150‑1 (MQ=60)
                                                                                   CTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCT                                                                   <  SRR19973074.573283/150‑1 (MQ=60)
                                                                                    TTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTC                                                                  <  SRR19973074.1157881/150‑1 (MQ=60)
                                                                                    TTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTC                                                                  <  SRR19973074.1157853/150‑1 (MQ=60)
                                                                                    TTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTC                                                                  <  SRR19973074.1157768/150‑1 (MQ=60)
                                                                                    TTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTC                                                                  >  SRR19973074.3873723/1‑150 (MQ=60)
                                                                                    TTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTC                                                                  >  SRR19973074.3873776/1‑150 (MQ=60)
                                                                                    TTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTC                                                                  >  SRR19973074.3873848/1‑150 (MQ=60)
                                                                                         CGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTA                                                             <  SRR19973074.3783598/150‑1 (MQ=60)
                                                                                         CGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTA                                                             <  SRR19973074.3783593/150‑1 (MQ=60)
                                                                                         CGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTA                                                             <  SRR19973074.4137165/150‑1 (MQ=60)
                                                                                         CGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTA                                                             <  SRR19973074.4137070/150‑1 (MQ=60)
                                                                                          GCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTAC                                                            <  SRR19973074.3840899/150‑1 (MQ=60)
                                                                                              CCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAG                                                        <  SRR19973074.804770/150‑1 (MQ=60)
                                                                                               CGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGC                                                       >  SRR19973074.1160553/1‑150 (MQ=60)
                                                                                               CGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGC                                                       >  SRR19973074.2374120/1‑150 (MQ=60)
                                                                                                      CACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCT                                                >  SRR19973074.2420967/1‑150 (MQ=60)
                                                                                                         CAGCCTGGTATTATTAGAGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAATACATGATTAAACTGGTCGAGTTTGATAACAAATAATATCTCTATTAAAAAGCGATTGCGCCA                                             >  SRR19973074.4034060/1‑150 (MQ=60)
                                                                                                          AGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAG                                            >  SRR19973074.2638603/1‑150 (MQ=60)
                                                                                                          AGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAG                                            >  SRR19973074.2639397/1‑150 (MQ=60)
                                                                                                          AGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAG                                            >  SRR19973074.2639401/1‑150 (MQ=60)
                                                                                                          AGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAG                                            >  SRR19973074.2639413/1‑150 (MQ=60)
                                                                                                           GCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGA                                           <  SRR19973074.1614732/150‑1 (MQ=60)
                                                                                                           GCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGA                                           >  SRR19973074.162025/1‑150 (MQ=60)
                                                                                                           GCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGA                                           >  SRR19973074.162137/1‑150 (MQ=60)
                                                                                                               GGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATT                                       >  SRR19973074.432781/1‑150 (MQ=60)
                                                                                                               GGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATT                                       >  SRR19973074.3160989/1‑150 (MQ=60)
                                                                                                               GGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATT                                       >  SRR19973074.63091/1‑150 (MQ=60)
                                                                                                                 TATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGC                                     >  SRR19973074.1822965/1‑150 (MQ=60)
                                                                                                                   TTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCT                                   >  SRR19973074.2362545/1‑150 (MQ=60)
                                                                                                                     ATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTC                                 >  SRR19973074.600174/1‑150 (MQ=60)
                                                                                                                        AGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATT                              >  SRR19973074.32776/1‑150 (MQ=60)
                                                                                                                        AGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATT                              >  SRR19973074.32753/1‑150 (MQ=60)
                                                                                                                        AGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATT                              >  SRR19973074.294001/1‑150 (MQ=60)
                                                                                                                          TGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCG                            >  SRR19973074.1469239/1‑150 (MQ=60)
                                                                                                                           GATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGC                           >  SRR19973074.2786603/1‑150 (MQ=60)
                                                                                                                           GATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGC                           >  SRR19973074.1269710/1‑150 (MQ=60)
                                                                                                                            ATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCG                          <  SRR19973074.2254310/150‑1 (MQ=60)
                                                                                                                            ATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCG                          <  SRR19973074.2254358/150‑1 (MQ=60)
                                                                                                                            ATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCG                          <  SRR19973074.2254421/150‑1 (MQ=60)
                                                                                                                              ATTGGCATCGGTACATTTGTCGAACCACGCCATCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTTGTCGAGTTTTATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTTCCTTCATACGCGCT                        >  SRR19973074.1379747/1‑150 (MQ=60)
                                                                                                                              ATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCT                        >  SRR19973074.169727/1‑150 (MQ=60)
                                                                                                                                 GGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACC                     <  SRR19973074.2344284/150‑1 (MQ=60)
                                                                                                                                     TCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCT                 <  SRR19973074.1926350/150‑1 (MQ=60)
                                                                                                                                      CGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTG                <  SRR19973074.1716240/150‑1 (MQ=60)
                                                                                                                                       GGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGC               >  SRR19973074.2688692/1‑150 (MQ=60)
                                                                                                                                       GGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGC               >  SRR19973074.909815/1‑150 (MQ=60)
                                                                                                                                             TTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGT         <  SRR19973074.3126344/150‑1 (MQ=60)
                                                                                                                                               TGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGA       <  SRR19973074.925155/150‑1 (MQ=60)
                                                                                                                                                  CGAACCACGCCAGCAAGGCGGCAAACTGACTGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGG    <  SRR19973074.517093/150‑1 (MQ=60)
                                                                                                                                                  CGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGG    <  SRR19973074.3256469/150‑1 (MQ=60)
                                                                                                                                                  CGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGG    <  SRR19973074.3256470/150‑1 (MQ=60)
                                                                                                                                                   GAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGC   >  SRR19973074.478989/1‑150 (MQ=60)
                                                                                                                                                    AACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCT  <  SRR19973074.2911011/150‑1 (MQ=60)
                                                                                                                                                    AACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCT  <  SRR19973074.2328181/150‑1 (MQ=60)
                                                                                                                                                    AACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCT  >  SRR19973074.3287563/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
GGACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCT  >  CP009273/1770132‑1770429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑