Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F434 I0 R1	1130	215.9	7031662	97.1%	6827743	147.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,770,281	T→A	100%	D146E (GAT→GAA)	ydiF →	putative acetyl‑CoA:acetoacetyl‑CoA transferase: alpha subunit/beta subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,770,281	0	T	A	99.2%	834.4 / ‑2.7	251	D146E (GAT→GAA)	ydiF	putative acetyl‑CoA:acetoacetyl‑CoA transferase: alpha subunit/beta subunit
Reads supporting (aligned to +/- strand): ref base T (1/1); new base A (132/117); total (133/118)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 251 total aligned reads displayed.

AATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTG  >  CP009273/1770136‑1770423

aaTCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAAcc                                                                                                                                              <  1:1089437/148‑1 (MQ=255)

aaTCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAAcc                                                                                                                                              >  1:1951875/1‑148 (MQ=255)

  tCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCAc                                                                                                                                            >  2:2911014/1‑148 (MQ=255)

    gccgcGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGc                                                                                                                                          <  2:41841/148‑1 (MQ=255)

          tATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCaa                                                                                                                                    >  2:1653606/1‑148 (MQ=255)

           aTTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAg                                                                                                                                   >  1:1148303/1‑148 (MQ=255)

            tttCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAgg                                                                                                                                  >  1:193356/1‑148 (MQ=255)

             ttCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAggc                                                                                                                                 >  1:807416/1‑148 (MQ=255)

                   aCTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCaaa                                                                                                                           >  2:2074348/1‑148 (MQ=255)

                   aCTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCaaa                                                                                                                           <  2:3221224/148‑1 (MQ=255)

                   aCTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCaaa                                                                                                                           <  1:3360854/148‑1 (MQ=255)

                      cGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACtg                                                                                                                        >  1:758272/1‑148 (MQ=255)

                        cAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACtgaa                                                                                                                      <  2:1940031/148‑1 (MQ=255)

                           aaCAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACtgaatga                                                                                                                   <  2:469566/148‑1 (MQ=255)

                           aaCAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACtgaatga                                                                                                                   >  1:1480117/1‑148 (MQ=255)

                           aaCAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACtgaatga                                                                                                                   >  1:780533/1‑148 (MQ=255)

                           aaCAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACtgaatga                                                                                                                   <  2:3005858/148‑1 (MQ=255)

                                aataaaatTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCa                                                                                                              <  1:2819314/148‑1 (MQ=255)

                                aataaaatTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCa                                                                                                              <  1:3095501/148‑1 (MQ=255)

                                   aaaatTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTa                                                                                                           >  2:259605/1‑148 (MQ=255)

                                    aaatTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTaa                                                                                                          >  2:974872/1‑148 (MQ=255)

                                    aaatTAGTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTaa                                                                                                          <  2:2274186/148‑1 (MQ=255)

                                     aatTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTaaa                                                                                                         <  2:793793/148‑1 (MQ=255)

                                     aatTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTaaa                                                                                                         <  1:1125106/148‑1 (MQ=255)

                                      attattGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAaag                                                                                                        >  2:1338957/1‑148 (MQ=255)

                                       ttattGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAaaga                                                                                                       >  1:811216/1‑148 (MQ=255)

                                           tGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCTCGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGAc                                                                                                   >  2:1162277/1‑148 (MQ=255)

                                           tGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGAc                                                                                                   >  2:560709/1‑148 (MQ=255)

                                            gCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGAcc                                                                                                  <  1:2906782/148‑1 (MQ=255)

                                               tataACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGa                                                                                               <  1:3423372/148‑1 (MQ=255)

                                               tataACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGa                                                                                               >  1:3408920/1‑148 (MQ=255)

                                                  aaCTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTa                                                                                            <  2:1959389/148‑1 (MQ=255)

                                                   aCTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTaa                                                                                           <  1:2117845/148‑1 (MQ=255)

                                                   aCTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTaa                                                                                           >  2:772354/1‑148 (MQ=255)

                                                    cTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTaaa                                                                                          <  2:3227698/148‑1 (MQ=255)

                                                    cTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTaaa                                                                                          >  1:1085632/1‑148 (MQ=255)

                                                     tACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAAc                                                                                         >  1:2839595/1‑148 (MQ=255)

                                                       cccACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTg                                                                                       >  2:2399486/1‑148 (MQ=255)

                                                         cacaAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGt                                                                                     >  2:301254/1‑148 (MQ=255)

                                                          acaAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTc                                                                                    >  1:3382746/1‑148 (MQ=255)

                                                           caAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCg                                                                                   <  2:2457050/148‑1 (MQ=255)

                                                             aGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACTTGATTAAACTGGTCGAg                                                                                 <  1:18180/148‑1 (MQ=255)

                                                             aGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAg                                                                                 <  1:3187580/148‑1 (MQ=255)

                                                             aGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAg                                                                                 <  1:19485/148‑1 (MQ=255)

                                                              ggTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGt                                                                                >  2:1661788/1‑148 (MQ=255)

                                                                          caAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAg                                                                    >  1:496012/1‑148 (MQ=255)

                                                                           aaaCCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGa                                                                   <  2:1042221/148‑1 (MQ=255)

                                                                           aaaCCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGa                                                                   <  2:326411/148‑1 (MQ=255)

                                                                           aaaCCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGa                                                                   <  2:2796701/148‑1 (MQ=255)

                                                                             aCCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAat                                                                 <  2:1729630/148‑1 (MQ=255)

                                                                             aCCTTACGCGCCGCCGCAGCCCACCAGCATTGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTTAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAat                                                                 <  1:3255047/148‑1 (MQ=255)

                                                                              ccTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGAATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAata                                                                <  2:895450/148‑1 (MQ=255)

                                                                                 tACGCGCCGCCGCATCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATAtct                                                             <  2:491537/148‑1 (MQ=255)

                                                                                 tACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATAtct                                                             <  2:1846649/148‑1 (MQ=255)

                                                                                    gcgcCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTa                                                          <  1:1879479/148‑1 (MQ=255)

                                                                                    gcgcCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTa                                                          <  1:68312/148‑1 (MQ=255)

                                                                                    gcgcCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTa                                                          <  1:1172226/148‑1 (MQ=255)

                                                                                    gcgcCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTa                                                          <  2:1553988/148‑1 (MQ=255)

                                                                                     cgccgccgcAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTAt                                                         >  1:2487286/1‑148 (MQ=255)

                                                                                     cgccgccgcAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTAt                                                         <  2:1783352/148‑1 (MQ=255)

                                                                                     cgccgccgcAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTAt                                                         >  1:10034/1‑148 (MQ=255)

                                                                                      gccgccgcAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTAtt                                                        <  1:459982/148‑1 (MQ=255)

                                                                                         gccgcAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCTAGTTTGATAACAAAGAATATCTCTATTACa                                                     <  1:2154717/148‑1 (MQ=255)

                                                                                         gccgcAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACa                                                     <  2:1269251/148‑1 (MQ=255)

                                                                                               gCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGa                                               <  2:340514/148‑1 (MQ=255)

                                                                                               gCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGa                                               >  1:2989121/1‑148 (MQ=255)

                                                                                                cccACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGAt                                              >  1:3309099/1‑148 (MQ=255)

                                                                                                cccACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGAt                                              >  2:2892077/1‑148 (MQ=255)

                                                                                                 ccaccaGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGAtt                                             <  2:3452541/148‑1 (MQ=255)

                                                                                                  caccaGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTg                                            >  1:2927823/1‑148 (MQ=255)

                                                                                                   accaGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTgc                                           >  1:3061973/1‑148 (MQ=255)

                                                                                                    ccaGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGa                                               <  1:1524058/143‑1 (MQ=255)

                                                                                                     caGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTgcgc                                         <  1:1958021/148‑1 (MQ=255)

                                                                                                       gCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCa                                       >  2:954197/1‑148 (MQ=255)

                                                                                                       gCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCa                                       >  2:2856318/1‑148 (MQ=255)

                                                                                                        ccTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAg                                      <  2:2384171/148‑1 (MQ=255)

                                                                                                          tGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGat                                    <  1:2316969/148‑1 (MQ=255)

                                                                                                          tGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGat                                    >  1:2779332/1‑148 (MQ=255)

                                                                                                          tGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGat                                    <  1:2673426/148‑1 (MQ=255)

                                                                                                           ggTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGata                                   <  1:2085608/148‑1 (MQ=255)

                                                                                                              attattAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTg                                >  1:1717369/1‑148 (MQ=255)

                                                                                                                tattaGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGcc                              <  2:723210/148‑1 (MQ=255)

                                                                                                                  ttaGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCtt                            <  2:1951875/148‑1 (MQ=255)

                                                                                                                   taGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTc                           >  1:2375962/1‑148 (MQ=255)

                                                                                                                    aGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCa                          >  1:2951612/1‑148 (MQ=255)

                                                                                                                    aGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCa                          <  2:2374528/148‑1 (MQ=255)

                                                                                                                    aGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCa                          <  2:1513138/148‑1 (MQ=255)

                                                                                                                        atatTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTcg                      >  1:3469813/1‑148 (MQ=255)

                                                                                                                        atatTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTcg                      <  2:2540453/148‑1 (MQ=255)

                                                                                                                                 tCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTaccac             >  2:3308394/1‑148 (MQ=255)

                                                                                                                                  cGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTaccacc            >  2:2833292/1‑148 (MQ=255)

                                                                                                                                  cGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTaccacc            >  2:1649851/1‑148 (MQ=255)

                                                                                                                                    ggACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTg          >  2:224559/1‑148 (MQ=255)

                                                                                                                                    ggACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTg          >  1:1370345/1‑148 (MQ=255)

                                                                                                                                    ggACATGTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTg          <  2:2899313/148‑1 (MQ=255)

                                                                                                                                        aTTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGAc      >  1:949979/1‑148 (MQ=255)

                                                                                                                                          ttGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAg    >  1:2867483/1‑148 (MQ=255)

                                                                                                                                          ttGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAg    >  1:1419623/1‑148 (MQ=255)

                                                                                                                                            gTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTg  <  2:715858/148‑1 (MQ=255)

                                                                                                                                            gTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTg  <  1:974872/148‑1 (MQ=255)

AATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTG  >  CP009273/1770136‑1770423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTA  >  CP009273/1770134‑1770430

ACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACC                                                                                                                                                     <  SRR19973065.1447504/150‑1 (MQ=60)

ACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACC                                                                                                                                                     >  SRR19973065.1964633/1‑150 (MQ=60)

  AATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCAC                                                                                                                                                   <  SRR19973065.1096317/150‑1 (MQ=60)

  AATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCAC                                                                                                                                                   <  SRR19973065.1096508/150‑1 (MQ=60)

  AATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCAC                                                                                                                                                   <  SRR19973065.1096615/150‑1 (MQ=60)

        CGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGC                                                                                                                                             >  SRR19973065.2309285/1‑150 (MQ=60)

        CGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGC                                                                                                                                             >  SRR19973065.3351251/1‑150 (MQ=60)

        CGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGC                                                                                                                                             >  SRR19973065.2113629/1‑150 (MQ=60)

           GTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAG                                                                                                                                          >  SRR19973065.1155705/1‑150 (MQ=60)

            TATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGG                                                                                                                                         >  SRR19973065.194862/1‑150 (MQ=60)

            TAGTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGG                                                                                                                                         >  SRR19973065.194904/1‑150 (MQ=60)

             ATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGC                                                                                                                                        >  SRR19973065.812948/1‑150 (MQ=60)

             ATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGC                                                                                                                                        >  SRR19973065.812748/1‑150 (MQ=60)

              TTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCG                                                                                                                                       >  SRR19973065.4754/1‑150 (MQ=60)

              TTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCG                                                                                                                                       <  SRR19973065.1923841/150‑1 (MQ=60)

              TTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCG                                                                                                                                       <  SRR19973065.3235073/150‑1 (MQ=60)

                  TGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAA                                                                                                                                   >  SRR19973065.1966305/1‑150 (MQ=60)

                   GAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAA                                                                                                                                  >  SRR19973065.1523013/1‑150 (MQ=60)

                     ACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACT                                                                                                                                <  SRR19973065.3381495/150‑1 (MQ=60)

                     ACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACT                                                                                                                                <  SRR19973065.548288/150‑1 (MQ=60)

                     ACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACT                                                                                                                                <  SRR19973065.3381401/150‑1 (MQ=60)

                      CTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTG                                                                                                                               >  SRR19973065.763325/1‑150 (MQ=60)

                      CTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTG                                                                                                                               >  SRR19973065.763550/1‑150 (MQ=60)

                      CTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTG                                                                                                                               <  SRR19973065.2840452/150‑1 (MQ=60)

                           AGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGA                                                                                                                          >  SRR19973065.785715/1‑150 (MQ=60)

                           AGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGA                                                                                                                          >  SRR19973065.1489574/1‑150 (MQ=60)

                                  AATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACT                                                                                                                   <  SRR19973065.3114404/150‑1 (MQ=60)

                                  AATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACT                                                                                                                   <  SRR19973065.2836115/150‑1 (MQ=60)

                                  AATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACT                                                                                                                   <  SRR19973065.2836730/150‑1 (MQ=60)

                                   ATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTA                                                                                                                  <  SRR19973065.37552/150‑1 (MQ=60)

                                       AATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGA                                                                                                              <  SRR19973065.1132667/150‑1 (MQ=60)

                                       AATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGA                                                                                                              <  SRR19973065.1132429/150‑1 (MQ=60)

                                       AATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGA                                                                                                              >  SRR19973065.816569/1‑150 (MQ=60)

                                          TATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGA                                                                                                           >  SRR19973065.2444588/1‑150 (MQ=60)

                                          TATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGA                                                                                                           >  SRR19973065.2103599/1‑150 (MQ=60)

                                           ATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGAC                                                                                                          >  SRR19973065.81600/1‑150 (MQ=60)

                                             TGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCT                                                                                                        >  SRR19973065.1004202/1‑150 (MQ=60)

                                              GCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTG                                                                                                       <  SRR19973065.2924493/150‑1 (MQ=60)

                                               CTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGA                                                                                                      >  SRR19973065.623554/1‑150 (MQ=60)

                                               CTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGA                                                                                                      >  SRR19973065.3430191/1‑150 (MQ=60)

                                               CTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGA                                                                                                      >  SRR19973065.3429750/1‑150 (MQ=60)

                                               CTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGA                                                                                                      >  SRR19973065.3473591/1‑150 (MQ=60)

                                               CTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGA                                                                                                      >  SRR19973065.2706127/1‑150 (MQ=60)

                                                TTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGAT                                                                                                     >  SRR19973065.2957233/1‑150 (MQ=60)

                                                TTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGAT                                                                                                     >  SRR19973065.2957490/1‑150 (MQ=60)

                                                TTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGAT                                                                                                     <  SRR19973065.2155545/150‑1 (MQ=60)

                                                TTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGAT                                                                                                     >  SRR19973065.1125106/1‑150 (MQ=60)

                                                 TATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATT                                                                                                    <  SRR19973065.3444273/150‑1 (MQ=60)

                                                  ATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTA                                                                                                   <  SRR19973065.831064/150‑1 (MQ=60)

                                                    AACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAA                                                                                                 >  SRR19973065.1092788/1‑150 (MQ=60)

                                                     ACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAAC                                                                                                >  SRR19973065.2857062/1‑150 (MQ=60)

                                                     ACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAAC                                                                                                <  SRR19973065.2131474/150‑1 (MQ=60)

                                                     ACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAAC                                                                                                >  SRR19973065.2857078/1‑150 (MQ=60)

                                                       TACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG                                                                                              <  SRR19973065.1957045/150‑1 (MQ=60)

                                                        ACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGG                                                                                             >  SRR19973065.1734129/1‑150 (MQ=60)

                                                         CCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGT                                                                                            >  SRR19973065.1476391/1‑150 (MQ=60)

                                                          CCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTC                                                                                           >  SRR19973065.3403428/1‑150 (MQ=60)

                                                              AAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGT                                                                                       >  SRR19973065.1441704/1‑150 (MQ=60)

                                                              AAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGT                                                                                       >  SRR19973065.3198675/1‑150 (MQ=60)

                                                              AAGGTGTACTTACACAAACCTTACGCGCCGACGCAGCCCACCAGCCTGGTATTATTCGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGT                                                                                       >  SRR19973065.3279484/1‑150 (MQ=60)

                                                               AGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTGTTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTT                                                                                      <  SRR19973065.2543331/150‑1 (MQ=60)

                                                               AGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACTTGATTAAACTGGTCGAGTT                                                                                      <  SRR19973065.18435/150‑1 (MQ=60)

                                                               AGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTT                                                                                      <  SRR19973065.3206817/150‑1 (MQ=60)

                                                               AGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTT                                                                                      >  SRR19973065.311730/1‑150 (MQ=60)

                                                               AGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTT                                                                                      <  SRR19973065.19754/150‑1 (MQ=60)

                                                                GGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTT                                                                                     >  SRR19973065.328683/1‑150 (MQ=60)

                                                                 GTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTG                                                                                    <  SRR19973065.3100094/150‑1 (MQ=60)

                                                                 GTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTG                                                                                    <  SRR19973065.188396/150‑1 (MQ=60)

                                                                  TGTACTTACACAAATCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGA                                                                                   <  SRR19973065.286761/150‑1 (MQ=60)

                                                                   GTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGAT                                                                                  >  SRR19973065.2064969/1‑150 (MQ=60)

                                                                          CACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAG                                                                           >  SRR19973065.750198/1‑150 (MQ=60)

                                                                          CACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAG                                                                           >  SRR19973065.499343/1‑150 (MQ=60)

                                                                              AACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATA                                                                       >  SRR19973065.1148867/1‑150 (MQ=60)

                                                                               ACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATAT                                                                      >  SRR19973065.155103/1‑150 (MQ=60)

                                                                               ACCTTACGCGCCGCCGCAGCCCACCAGCATTGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTTAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATAT                                                                      <  SRR19973065.3274731/150‑1 (MQ=60)

                                                                                CCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATC                                                                     <  SRR19973065.793964/150‑1 (MQ=60)

                                                                                CCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATC                                                                     <  SRR19973065.3405345/150‑1 (MQ=60)

                                                                                     CGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTAT                                                                >  SRR19973065.9842/1‑150 (MQ=60)

                                                                                     CGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTAT                                                                >  SRR19973065.10191/1‑150 (MQ=60)

                                                                                     CGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTAT                                                                >  SRR19973065.1591539/1‑150 (MQ=60)

                                                                                     CGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTAT                                                                >  SRR19973065.2502935/1‑150 (MQ=60)

                                                                                      GCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATT                                                               <  SRR19973065.2907285/150‑1 (MQ=60)

                                                                                      GCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATT                                                               <  SRR19973065.1179696/150‑1 (MQ=60)

                                                                                      GCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATT                                                               <  SRR19973065.69044/150‑1 (MQ=60)

                                                                                      GCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATT                                                               <  SRR19973065.69033/150‑1 (MQ=60)

                                                                                      GCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATT                                                               <  SRR19973065.1891851/150‑1 (MQ=60)

                                                                                      GCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATT                                                               <  SRR19973065.222887/150‑1 (MQ=60)

                                                                                        GCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTAC                                                             <  SRR19973065.463155/150‑1 (MQ=60)

                                                                                           GCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCTAGTTTGATAACAAAGAATATCTCTATTACAAA                                                          <  SRR19973065.2168575/150‑1 (MQ=60)

                                                                                            CCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAG                                                         <  SRR19973065.3077369/150‑1 (MQ=60)

                                                                                            CCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAG                                                         <  SRR19973065.2928733/150‑1 (MQ=60)

                                                                                            CCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAG                                                         <  SRR19973065.343428/150‑1 (MQ=60)

                                                                                              GCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCG                                                       <  SRR19973065.3400970/150‑1 (MQ=60)

                                                                                               CAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGA                                                      >  SRR19973065.3007419/1‑150 (MQ=60)

                                                                                                AGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGAT                                                     >  SRR19973065.1829982/1‑150 (MQ=60)

                                                                                                AGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGAT                                                     >  SRR19973065.3329266/1‑150 (MQ=60)

                                                                                                 tctCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATT                                                    <  SRR19973065.1534055/147‑1 (MQ=60)

                                                                                                  CCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTG                                                   >  SRR19973065.2945599/1‑150 (MQ=60)

                                                                                                   CCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGC                                                  >  SRR19973065.3080693/1‑150 (MQ=60)

                                                                                                       CAGCCTTGTATTATTAGTGATATTTGCATCGGGACATTTGTCGAACCAGGCCAGCAATGCGTCAAACTGAATGAAGTCACTATAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCA                                              <  SRR19973065.1964121/150‑1 (MQ=60)

                                                                                                       CAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCA                                              <  SRR19973065.1970811/150‑1 (MQ=60)

                                                                                                       CAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCA                                              <  SRR19973065.1970764/150‑1 (MQ=60)

                                                                                                       CAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCA                                              <  SRR19973065.1964418/150‑1 (MQ=60)

                                                                                                       CAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCA                                              <  SRR19973065.241422/150‑1 (MQ=60)

                                                                                                          CCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGAT                                           >  SRR19973065.2795099/1‑150 (MQ=60)

                                                                                                          CCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGAT                                           >  SRR19973065.2796580/1‑150 (MQ=60)

                                                                                                          CCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGAT                                           >  SRR19973065.1291430/1‑150 (MQ=60)

                                                                                                           CTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATA                                          <  SRR19973065.1032541/150‑1 (MQ=60)

                                                                                                            TGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATAT                                         >  SRR19973065.971665/1‑150 (MQ=60)

                                                                                                            TGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATAT                                         <  SRR19973065.2331495/150‑1 (MQ=60)

                                                                                                            TGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATAT                                         <  SRR19973065.2689991/150‑1 (MQ=60)

                                                                                                             GGTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATT                                        <  SRR19973065.2098979/150‑1 (MQ=60)

                                                                                                              GTATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTG                                       >  SRR19973065.1728785/1‑150 (MQ=60)

                                                                                                               TATTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGC                                      >  SRR19973065.3076830/1‑150 (MQ=60)

                                                                                                                 TTATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCT                                    <  SRR19973065.3001602/150‑1 (MQ=60)

                                                                                                                   ATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTC                                  >  SRR19973065.2390802/1‑150 (MQ=60)

                                                                                                                   ATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTC                                  >  SRR19973065.2390695/1‑150 (MQ=60)

                                                                                                                   ATTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTC                                  <  SRR19973065.981703/150‑1 (MQ=60)

                                                                                                                    TTAGTGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCA                                 >  SRR19973065.2969592/1‑150 (MQ=60)

                                                                                                                        TGATATTGGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCG                             >  SRR19973065.3490934/1‑150 (MQ=60)

                                                                                                                               GGCATCGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACC                      >  SRR19973065.457041/1‑150 (MQ=60)

                                                                                                                                    CGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTG                 >  SRR19973065.1379396/1‑150 (MQ=60)

                                                                                                                                    CGGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTG                 >  SRR19973065.770356/1‑150 (MQ=60)

                                                                                                                                     GGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGC                >  SRR19973065.1214155/1‑150 (MQ=60)

                                                                                                                                     GGGACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGC                >  SRR19973065.1169111/1‑150 (MQ=60)

                                                                                                                                        ACATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGAC             >  SRR19973065.956255/1‑150 (MQ=60)

                                                                                                                                          ATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAG           >  SRR19973065.2885074/1‑150 (MQ=60)

                                                                                                                                          ATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAG           >  SRR19973065.1428863/1‑150 (MQ=60)

                                                                                                                                          ATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAG           >  SRR19973065.1428890/1‑150 (MQ=60)

                                                                                                                                          ATTTGTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAG           >  SRR19973065.2887173/1‑150 (MQ=60)

                                                                                                                                              GTCGAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAA       <  SRR19973065.981347/150‑1 (MQ=60)

                                                                                                                                                 GAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGC    >  SRR19973065.1041829/1‑150 (MQ=60)

                                                                                                                                                 GAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGC    >  SRR19973065.1034899/1‑150 (MQ=60)

                                                                                                                                                 GAACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGC    >  SRR19973065.1034559/1‑150 (MQ=60)

                                                                                                                                                  AACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCT   <  SRR19973065.478499/150‑1 (MQ=60)

                                                                                                                                                  AACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCT   <  SRR19973065.2764646/150‑1 (MQ=60)

                                                                                                                                                   ACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTA  >  SRR19973065.96214/1‑150 (MQ=60)

                                                                                                                                                   ACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTA  <  SRR19973065.507862/150‑1 (MQ=60)

                                                                                                                                                   ACCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATATCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTA  <  SRR19973065.206818/150‑1 (MQ=60)

ACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTA  >  CP009273/1770134‑1770430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`