Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F121 I0 R1 856 273.6 9081716 94.1% 8545894 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,614,168 C→A 86.2% D22Y (GAT→TAT)  upp ← uracil phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,614,1680CA86.2% 614.6 / 90.4 260D22Y (GAT→TAT) uppuracil phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (21/15);  new base A (111/105);  total (132/128)
Fisher's exact test for biased strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 262 total aligned reads displayed.

AATTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCG  >  CP009273/2614019‑2614312
                                                                                                                                                     |                                                                                                                                                
  ttttCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATAtt                                                                                                                                                <  2:211975/150‑1 (MQ=255)
     tCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCt                                                                                                                                             >  2:4151752/1‑150 (MQ=255)
     tCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCt                                                                                                                                             <  2:2956719/150‑1 (MQ=255)
      cTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCATCGATAGTTAATTTTTACGTTTAGAGGTCGGCGGTCGCTTCGTAAGTAAGCAGGCAACCCACATCCGAACCGAGTTCGCGCAATCGCTTTGTGCTGTTATATTtctc                                                                                                                                            >  1:3643987/1‑150 (MQ=255)
       ttACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCAGATATCTTGCTCa                                                                                                                                           >  2:1700198/1‑150 (MQ=255)
       ttACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCACCCTTCGATAGTTACTTTTTACGTTTCGAGGTCGGCGGTCGCTTAGTAAGTCAGCACGCTACCCACATCGGAAGCCAGTTCGCGAAAGCGCTTGGTGCTGATATATTTCTCa                                                                                                                                           >  1:2123400/1‑150 (MQ=255)
        tACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCAc                                                                                                                                          <  2:4524817/150‑1 (MQ=255)
         aCCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACg                                                                                                                                         <  2:1537648/150‑1 (MQ=255)
         aCCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACg                                                                                                                                         <  1:2229279/150‑1 (MQ=255)
          ccTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGc                                                                                                                                        <  2:2908253/150‑1 (MQ=255)
          ccTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCATGGTGCTGATATATTTCTCACGc                                                                                                                                        >  2:4338036/1‑150 (MQ=255)
          ccTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACATCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGc                                                                                                                                        >  2:3131709/1‑150 (MQ=255)
           cTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCGTCGATAGTTAATTTTTCCGTTTAGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACATCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTTCTCACGCa                                                                                                                                       >  1:1789239/1‑150 (MQ=255)
             ttGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCGTCGATAGTTAATTTTTCCGTTTCGAGGTCGGCGGTCGCGTCGTAAGTAAGCAGGCTACCCACATAGGAAGCGAGTTAGCGAAACCGCATTGTGGTGATATATTTCTCACGCaac                                                                                                                                     >  1:4384500/1‑148 (MQ=255)
              tGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCa                                                                                                                                    <  1:1801587/150‑1 (MQ=255)
              tGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCa                                                                                                                                    <  1:4086784/150‑1 (MQ=255)
               gATCTTGTCGTTTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTAGGCTGTCGCTTCGTAAGTCTGCTGGATTCACAATTCTGATGCGAGTTCGAGTAAGCGCTTGGTGCTGATATATTGCTCTCGcttgag                                                                                                                                   <  1:3747887/150‑6 (MQ=255)
                aTCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGt                                                                                                                                  >  2:3002240/1‑150 (MQ=255)
                 tCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTc                                                                                                                                 <  2:3403809/150‑1 (MQ=255)
                 tCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTAATTTTTACGTTTCGATGTCGGCGGTCGCTTAGTAAGTCAGCAGGCTACCCACGACGGAAGCGAGTTAGCGAAAACGCATGGTGGTGATATATTTCTCACCCAACAATc                                                                                                                                 >  1:4350403/1‑150 (MQ=255)
                  cTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTcc                                                                                                                                <  1:2439313/150‑1 (MQ=255)
                  cTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTcc                                                                                                                                >  2:3874988/1‑150 (MQ=255)
                  cTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTcc                                                                                                                                >  2:2632217/1‑150 (MQ=255)
                  cTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTAGTAAGTCAGCAGGCTACCCACATCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTcc                                                                                                                                >  1:1075070/1‑150 (MQ=255)
                  cTGGTCGATTTCTACCGGGCCGTTCCAGCCGTCGATAGTTAATTTTTACGTTTAGAGGTCGGCGGGCGCGTAGTAAGTAAACAGGCAAACCACATCGGAACCGAGTTCGCGAAAGCCCATTGTGCTGATAAATTTCTCACCCaaaactcc                                                                                                                                >  1:4286236/1‑143 (MQ=255)
                     gTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTTCTCACGCATAAGTCCCAg                                                                                                                             >  2:4243749/1‑150 (MQ=255)
                       cGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCt                                                                                                                           >  2:3335395/1‑150 (MQ=255)
                        gATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGCGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCtt                                                                                                                          >  2:2408543/1‑150 (MQ=255)
                         aTTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTtg                                                                                                                         <  1:4151752/150‑1 (MQ=255)
                              tACCGGGCCGTTCCAGCCTTCGATAGTTACTTTGTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGttt                                                                                                                    <  1:4469007/150‑1 (MQ=255)
                                       gTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTgg                                                                                                           >  1:3854897/1‑150 (MQ=255)
                                         tCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGgt                                                                                                         <  2:822706/150‑1 (MQ=255)
                                              ccTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTg                                                                                                    >  2:2602183/1‑150 (MQ=255)
                                              ccTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTg                                                                                                    <  2:4479212/150‑1 (MQ=255)
                                               cTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGa                                                                                                   <  1:2839107/150‑1 (MQ=255)
                                               cTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGa                                                                                                   >  1:934390/1‑150 (MQ=255)
                                                  cGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACtt                                                                                                >  2:96020/1‑150 (MQ=255)
                                                  cGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACtt                                                                                                >  1:3150831/1‑150 (MQ=255)
                                                      aGTTCCTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCAc                                                                                            <  1:3393452/150‑1 (MQ=255)
                                                      aGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCAc                                                                                            <  2:3488284/150‑1 (MQ=255)
                                                       gTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACg                                                                                           <  1:4220998/150‑1 (MQ=255)
                                                       gTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACg                                                                                           <  2:1240187/150‑1 (MQ=255)
                                                         tACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCAACAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGAt                                                                                         >  1:3859909/1‑150 (MQ=255)
                                                                tCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATa                                                                                  >  2:2075093/1‑150 (MQ=255)
                                                                    tttCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATActct                                                                              >  1:4510817/1‑150 (MQ=255)
                                                                     ttCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCtt                                                                             >  1:4437022/1‑150 (MQ=255)
                                                                     ttCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCtt                                                                             >  1:2197396/1‑150 (MQ=255)
                                                                     ttCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCtt                                                                             <  2:3547408/150‑1 (MQ=255)
                                                                     ttCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTAGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCtt                                                                             >  2:389154/1‑150 (MQ=255)
                                                                      tCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCttt                                                                            <  2:1344927/150‑1 (MQ=255)
                                                                      tCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCttt                                                                            >  2:4299692/1‑150 (MQ=255)
                                                                      tCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCttt                                                                            <  1:4454882/150‑1 (MQ=255)
                                                                        gAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTtct                                                                          >  1:4392515/1‑150 (MQ=255)
                                                                        gAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTtct                                                                          <  2:1952860/150‑1 (MQ=255)
                                                                        gAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTtct                                                                          >  2:3065718/1‑150 (MQ=255)
                                                                        gAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTAACCACATCGGAAGCGATTTCGCGAAAGCGCATGGTGCTGATATATTTCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTtct                                                                          >  1:1198733/1‑150 (MQ=255)
                                                                         aGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTtctc                                                                         >  1:4433486/1‑150 (MQ=255)
                                                                           gTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCt                                                                       <  2:177739/150‑1 (MQ=255)
                                                                           gTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCt                                                                       <  1:496466/150‑1 (MQ=255)
                                                                                 ggTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAgg                                                                 <  1:2392575/150‑1 (MQ=255)
                                                                                    cGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGc                                                              >  2:875034/1‑150 (MQ=255)
                                                                                     gCTTCGTAAGTCAGCATGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGATTGGTGCTGATATATTGCTCACGCATCAGTCCAAGATTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCa                                                             <  1:316008/150‑1 (MQ=255)
                                                                                         cGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCca                                                         <  2:4025414/150‑1 (MQ=255)
                                                                                          gTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGcccc                                                        >  2:4410642/1‑148 (MQ=255)
                                                                                             aGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACaaa                                                     >  1:2130258/1‑150 (MQ=255)
                                                                                               tCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACaaaaa                                                   >  2:3704047/1‑150 (MQ=255)
                                                                                                 agcagGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACaaaaaaa                                                 >  2:1882708/1‑150 (MQ=255)
                                                                                                   cagGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAAt                                               >  2:2222609/1‑150 (MQ=255)
                                                                                                   cagGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAAt                                               >  2:3296255/1‑150 (MQ=255)
                                                                                                   cagGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAAt                                               <  1:2650847/150‑1 (MQ=255)
                                                                                                   cagGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAAt                                               >  2:1016541/1‑150 (MQ=255)
                                                                                                        tACCCACTTCGGATGCGTGTGCGCTAAAGCGCTTGTTGCTGATATTTTGCTCACGCATCAGTCCCAGTTTGTTTTTGACGTGTGGGTTTTTGACTTCCACTATATTAATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACg                                          <  1:4027191/150‑1 (MQ=255)
                                                                                                             aCTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACACAAAAAATCGACGGATta                                     >  1:167466/1‑150 (MQ=255)
                                                                                                                tCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATAc                                  >  1:2903283/1‑150 (MQ=255)
                                                                                                                tCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATAc                                  >  1:59971/1‑150 (MQ=255)
                                                                                                                 cGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATAcc                                 >  2:3448370/1‑150 (MQ=255)
                                                                                                                  ggAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATAcct                                <  2:1075070/150‑1 (MQ=255)
                                                                                                                    aaGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATAcctcc                              >  1:2153158/1‑150 (MQ=255)
                                                                                                                    aaGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATAcctcc                              >  1:2544229/1‑150 (MQ=255)
                                                                                                                     aGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTTTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTTAGGGGCAGCCACAAAAAAAATCGACGGATTATAcctcct                             <  2:1338799/150‑1 (MQ=255)
                                                                                                                      gCGTGTTCGCTAAAGCGCTTGGTGCTTATTTATTGCCCACGCATAATTCCCAGCTTTTGTTTTACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCCCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCtt                            <  2:787254/150‑1 (MQ=255)
                                                                                                                      gCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCtt                            <  2:712352/150‑1 (MQ=255)
                                                                                                                       cGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCttt                           >  2:1203896/1‑150 (MQ=255)
                                                                                                                       cGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCttt                           <  2:2153158/150‑1 (MQ=255)
                                                                                                                        gAGTTCGCGAACGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCAACAAAAAAAATCGACGGATTATACCTCCTttc                          <  2:119812/150‑1 (MQ=255)
                                                                                                                        gAGTGCGCGAAAGCGCTTGGTGCTTATATATGGCGCACGCATCAGTCCCAGCTTGTGTTTGACGAGGGGGTGTTTGCCTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTttc                          <  2:304894/150‑1 (MQ=255)
                                                                                                                          gTTCGCTAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTttctt                        <  2:1456943/150‑1 (MQ=255)
                                                                                                                           ttCGCGAACGCGCTTGGTGCTGATATTTTGCACCCGCACCCGTCCCAGCTTGTTTTTGACGAGGGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGTGGGGCCCCCACAAAAAAAATCGACGGATTATACCTCCTttcttc                       <  1:1584711/150‑1 (MQ=255)
                                                                                                                            tCGCTAAAGCGCTTGGTGATTATATATTGCACACGCATCAGTCCCTGATTGTTTTTGAGGTGTGGGTGTTTTACTTCCACTATCTTCATACTCTTTCTCCTTTGTGGGGCAGCCAAAAAAAAAATCGACGGATTATACCTCCTTTCTTCa                      <  1:462582/150‑1 (MQ=255)
                                                                                                                            tCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCa                      >  2:582139/1‑150 (MQ=255)
                                                                                                                              gcgAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGTGTGGGTTTTTGACTTCCACTATCTTCATACTCTTTCTCCTTTGTGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAg                    <  1:1344918/150‑1 (MQ=255)
                                                                                                                                   aGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAggcggc               >  1:4160388/1‑150 (MQ=255)
                                                                                                                                    gcgcTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCa              >  1:1073303/1‑150 (MQ=255)
                                                                                                                                      gtttGGTGCTTATATTTTGCTCACGAATCTGTCCCTGTTGTTTTTTGAGGTGGGGTTTTTTGATTTCCACTATCTTAATACTCTTTCTCGTTGGTGGGGCAGCAAAAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAat            <  1:3457714/148‑1 (MQ=255)
                                                                                                                                       cTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAata           <  2:1669016/150‑1 (MQ=255)
                                                                                                                                         tGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATAtt         >  2:1446912/1‑150 (MQ=255)
                                                                                                                                          ggTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTc        >  1:3067023/1‑150 (MQ=255)
                                                                                                                                             gCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCttt     <  2:1676955/150‑1 (MQ=255)
                                                                                                                                              cTGATATATTGCACACGCATCAGTCCCAGTTTGTGTTTGACGCGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCGCCTTTGAGGGGCAGCCAAAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCtttt    <  1:1683598/150‑1 (MQ=255)
                                                                                                                                               tGATATATTGCTCACGCATCTGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCATTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTc   <  2:3564899/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
AATTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCG  >  CP009273/2614019‑2614312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGAC  >  CP009273/2614021‑2614317
                                                                                                                                                   |                                                                                                                                                     
TTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATT                                                                                                                                                     <  SRR19973080.3201655/150‑1 (MQ=60)
TTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATT                                                                                                                                                     <  SRR19973080.2422557/150‑1 (MQ=60)
    CTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTC                                                                                                                                                 <  SRR19973080.4453276/150‑1 (MQ=60)
    CTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCATCGATAGTTAATTTTTACGTTTAGAGGTCGGCGGTCGCTTCGTAAGTAAGCAGGCAACCCACATCCGAACCGAGTTCGCGCAATCGCTTTGTGCTGTTATATTTCTC                                                                                                                                                 >  SRR19973080.3664101/1‑150 (MQ=60)
     TTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCATCGTAAGTCAGCAGGCAACCCCCATAGGAAGCGAGTTCGCGAAAACGCTTTGTGCGGATATATTTCTCA                                                                                                                                                >  SRR19973080.2135559/1‑150 (MQ=60)
     TTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCACCCTTCGATAGTTACTTTTTACGTTTCGAGGTCGGCGGTCGCTTAGTAAGTCAGCACGCTACCCACATCGGAAGCCAGTTCGCGAAAGCGCTTGGTGCTGATATATTTCTCA                                                                                                                                                >  SRR19973080.2135371/1‑150 (MQ=60)
       ACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACG                                                                                                                                              <  SRR19973080.3671141/150‑1 (MQ=60)
       ACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACG                                                                                                                                              <  SRR19973080.2242244/150‑1 (MQ=60)
       ACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACG                                                                                                                                              <  SRR19973080.2241555/150‑1 (MQ=60)
       ACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACATAGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACG                                                                                                                                              >  SRR19973080.2992841/1‑150 (MQ=60)
         CTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCA                                                                                                                                            <  SRR19973080.3890298/150‑1 (MQ=60)
         CTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCGTCGATAGTTAATTTTTCCGTTTAGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACATCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTTCTCACGCA                                                                                                                                            >  SRR19973080.1799447/1‑150 (MQ=60)
           TTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCGTCGATAGTTAATTTTTCCGTTTCGAGGTCGGCGGTCGCGTCGTAAGTAAGCAGGCTACCCACATAGGAAGCGAGTTAGCGAAACCGCATTGTGGTGATATATTTCTCACGCAAC                                                                                                                                          >  SRR19973080.4408391/1‑150 (MQ=60)
            TGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCA                                                                                                                                         <  SRR19973080.4109042/150‑1 (MQ=60)
            TGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCA                                                                                                                                         <  SRR19973080.4108854/150‑1 (MQ=60)
            TGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCA                                                                                                                                         <  SRR19973080.1811839/150‑1 (MQ=60)
            TGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCA                                                                                                                                         <  SRR19973080.1811826/150‑1 (MQ=60)
             GATCTTGTCGTTTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTAGGCTGTCGCTTCGTAAGTCTGCTGGATTCACAATTCTGATGCGAGTTCGAGTAAGCGCTTGGTGCTGATATATTGCTCtcgcttgag                                                                                                                                        <  SRR19973080.3768360/150‑10 (MQ=60)
               TCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTC                                                                                                                                      <  SRR19973080.3878058/150‑1 (MQ=60)
               TCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTAGTAAGTAAGCAGGCTACCCACATCGGAAGCGATTTCGCGAAAGCGCTTGGTGGTGATATATTTCTCAAGCAACAGTC                                                                                                                                      >  SRR19973080.4374160/1‑150 (MQ=60)
                CTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCC                                                                                                                                     <  SRR19973080.2452830/150‑1 (MQ=60)
                CTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTAGTAAGTCAGCAGGCTACCCACATCGGAAGCGAGTTCGCGAAAGCGCTTTGTGCTGATATATTGCTCACGCATCAGTCC                                                                                                                                     >  SRR19973080.1081324/1‑150 (MQ=60)
                       ATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTG                                                                                                                              <  SRR19973080.4174180/150‑1 (MQ=60)
                            TACCGGGCCGTTCCAGCCTTCGATAGTTACTTTGTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTT                                                                                                                         <  SRR19973080.4493213/150‑1 (MQ=60)
                               CGGGCCGTTCCAGCCGTCGATAGTTACTTTTTACGTTTCGAGGTCGGCGGTCGCGTCGTAAGTCAACAGGCTACCCACAACGGAAGCGACTTCGCGAAAGCGCTTGGTGCGGATATATTGCTCACGCATCAGTCCCACCATGTGTTTGAA                                                                                                                      >  SRR19973080.4195025/1‑150 (MQ=60)
                                  GCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAG                                                                                                                   <  SRR19973080.4430700/150‑1 (MQ=60)
                                   CCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGT                                                                                                                  <  SRR19973080.1713531/150‑1 (MQ=60)
                                     GTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGG                                                                                                                >  SRR19973080.3876104/1‑150 (MQ=60)
                                     GTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGG                                                                                                                >  SRR19973080.3876107/1‑150 (MQ=60)
                                         CAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGT                                                                                                            <  SRR19973080.1954982/150‑1 (MQ=60)
                                            CCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTG                                                                                                         >  SRR19973080.2775713/1‑150 (MQ=60)
                                            CCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGACGCTTCGTAAGTCACCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCATTGTGCTGATATCTTTCTCACGCATCAGTACCAGCTTGTGTTTGACGAGTGGGTGTTTG                                                                                                         >  SRR19973080.2773824/1‑150 (MQ=60)
                                             CTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGA                                                                                                        >  SRR19973080.940101/1‑150 (MQ=60)
                                             CTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGA                                                                                                        <  SRR19973080.2854702/150‑1 (MQ=60)
                                                CGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTT                                                                                                     >  SRR19973080.3168188/1‑150 (MQ=60)
                                                CGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTT                                                                                                     <  SRR19973080.1321758/150‑1 (MQ=60)
                                                  ATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCC                                                                                                   >  SRR19973080.4471667/1‑150 (MQ=60)
                                                  ATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCC                                                                                                   >  SRR19973080.2188609/1‑150 (MQ=60)
                                                    AGTTCCTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCAC                                                                                                 <  SRR19973080.3411791/150‑1 (MQ=60)
                                                     GTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACG                                                                                                <  SRR19973080.4243964/150‑1 (MQ=60)
                                                     GTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACG                                                                                                <  SRR19973080.307562/150‑1 (MQ=60)
                                                       TACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCAACAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGAT                                                                                              >  SRR19973080.3881156/1‑150 (MQ=60)
                                                            TTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTTCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCA                                                                                         >  SRR19973080.1168390/1‑150 (MQ=60)
                                                               CCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCAATTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATAC                                                                                      <  SRR19973080.4561113/150‑1 (MQ=60)
                                                                  TTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCT                                                                                   >  SRR19973080.4535161/1‑150 (MQ=60)
                                                                   TTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTT                                                                                  >  SRR19973080.2209587/1‑150 (MQ=60)
                                                                   TTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTT                                                                                  >  SRR19973080.4461116/1‑150 (MQ=60)
                                                                    TCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTT                                                                                 <  SRR19973080.4479037/150‑1 (MQ=60)
                                                                    TCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTT                                                                                 <  SRR19973080.4479163/150‑1 (MQ=60)
                                                                    TCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTT                                                                                 <  SRR19973080.4479310/150‑1 (MQ=60)
                                                                      GAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCT                                                                               >  SRR19973080.4416435/1‑150 (MQ=60)
                                                                      GAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCT                                                                               >  SRR19973080.2152328/1‑150 (MQ=60)
                                                                      GAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCT                                                                               >  SRR19973080.494449/1‑150 (MQ=60)
                                                                      GAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTAGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCT                                                                               >  SRR19973080.497193/1‑150 (MQ=60)
                                                                      GAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTAACCACATCGGAAGCGATTTCGCGAAAGCGCATGGTGCTGATATATTTCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATAATCTTTAT                                                                               >  SRR19973080.1205958/1‑150 (MQ=60)
                                                                       AGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTC                                                                              <  SRR19973080.1690392/150‑1 (MQ=60)
                                                                       AGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTC                                                                              >  SRR19973080.4457567/1‑150 (MQ=60)
                                                                       AGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTC                                                                              >  SRR19973080.4457768/1‑150 (MQ=60)
                                                                        GGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCC                                                                             >  SRR19973080.2141894/1‑150 (MQ=60)
                                                                         GTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCT                                                                            <  SRR19973080.4376954/150‑1 (MQ=60)
                                                                         GTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCT                                                                            <  SRR19973080.499621/150‑1 (MQ=60)
                                                                         GTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCT                                                                            <  SRR19973080.499676/150‑1 (MQ=60)
                                                                               GGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGG                                                                      <  SRR19973080.2405912/150‑1 (MQ=60)
                                                                               GGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGG                                                                      <  SRR19973080.2405865/150‑1 (MQ=60)
                                                                               GGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGG                                                                      >  SRR19973080.787131/1‑150 (MQ=60)
                                                                                   GCTTCGTAAGTCAGCATGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGATTGGTGCTGATATATTGCTCACGCATCAGTCCAAGATTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCA                                                                  <  SRR19973080.317977/150‑1 (MQ=60)
                                                                                   GCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCA                                                                  <  SRR19973080.948756/150‑1 (MQ=60)
                                                                                   GCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCA                                                                  <  SRR19973080.948783/150‑1 (MQ=60)
                                                                                   GCTTCGTAAGTCAGCAGGCGACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCA                                                                  <  SRR19973080.317468/150‑1 (MQ=60)
                                                                                        GTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC                                                             <  SRR19973080.198882/150‑1 (MQ=60)
                                                                                         TAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACA                                                            <  SRR19973080.3659740/150‑1 (MQ=60)
                                                                                         TAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACA                                                            >  SRR19973080.2517228/1‑150 (MQ=60)
                                                                                           AGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAA                                                          >  SRR19973080.2142254/1‑150 (MQ=60)
                                                                                                 CAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAAT                                                    <  SRR19973080.2560253/150‑1 (MQ=60)
                                                                                                 CAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAAT                                                    <  SRR19973080.2665155/150‑1 (MQ=60)
                                                                                                  AGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATC                                                   >  SRR19973080.157027/1‑150 (MQ=60)
                                                                                                   GGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCG                                                  <  SRR19973080.3438288/150‑1 (MQ=60)
                                                                                                      TACCCACTTCGGATGCGTGTGCGCTAAAGCGCTTGTTGCTGATATTTTGCTCACGCATCAGTCCCAGTTTGTTTTTGACGTGTGGGTTTTTGACTTCCACTATATTAATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACG                                               <  SRR19973080.4049259/150‑1 (MQ=60)
                                                                                                         ccatttgggaagggtGTTCGCGAAAGCGCTTGGTGCTTATTTTTTGCTCACGCATCAGTCCCAGCTTGTTTTTGACGTGTGGGTGTTTGACTTCCACTATCTTCATACTGTTTCTCCTTTGCGGGGCAGCCACAAAAAAAATCGACGGAT                                            <  SRR19973080.4130219/135‑1 (MQ=60)
                                                                                                         CCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGAT                                            >  SRR19973080.321309/1‑150 (MQ=60)
                                                                                                          CACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATT                                           >  SRR19973080.4069716/1‑150 (MQ=60)
                                                                                                           ACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACACAAAAAATCGACGGATTA                                          >  SRR19973080.168795/1‑150 (MQ=60)
                                                                                                             ttcggacgcttgttcgctaatgcggTTGGTGCTGATATATTGCTCACGCATCAGTCCCTGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACTATCTTAATACTCTTTCTCCTTTGTGGGGCAGCCAAAAAAAAAATCGACGGATTATA                                        <  SRR19973080.4411735/125‑1 (MQ=60)
                                                                                                              TCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATAC                                       >  SRR19973080.2919128/1‑150 (MQ=60)
                                                                                                              TCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATAC                                       >  SRR19973080.4185979/1‑150 (MQ=60)
                                                                                                              TCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATAC                                       >  SRR19973080.60573/1‑150 (MQ=60)
                                                                                                              TCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATAC                                       >  SRR19973080.3841792/1‑150 (MQ=60)
                                                                                                              TCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATAC                                       >  SRR19973080.644083/1‑150 (MQ=60)
                                                                                                                GTAAGCGTGTTCGCGAAAGCGGTTGGTGCTTATATTTTGCTCACGCATCAGTCCCAGCTTGTTTTTGACGTGTGGGTTTTTGACTTCCACGATCTTCATACTCTTTCTCATTTGAGGGGCAGCCAAAAAAAAAATCGACGGATTATACCT                                     <  SRR19973080.4460442/150‑1 (MQ=60)
                                                                                                                GTAAGCGAGTTCGCGAAAGGGATTGGTGCTTATATATTGCTCACGCATCTGTCCCTGGTTGTGTTTGAGGAGTGGGTTTTTGACTTCCACTATCTTCATACTATTTCTCCTTTGTGGGGCAGCCAAAAAAAAAATCGACGGATTATACCT                                     <  SRR19973080.4513639/150‑1 (MQ=60)
                                                                                                                GGAAGCGTGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCTGTCCCTGCTTGTGTTTGACGTGTGGGTGTTTTACTTCCACTATATTCATACTCTTTCTCCTTTGAGGGGCTGCCACAAAAAAAATCGACGGATTATACCT                                     <  SRR19973080.4495906/150‑1 (MQ=60)
                                                                                                                GGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCT                                     >  SRR19973080.3593362/1‑150 (MQ=60)
                                                                                                                GGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCT                                     >  SRR19973080.2202379/1‑150 (MQ=60)
                                                                                                                GGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCT                                     >  SRR19973080.2909707/1‑150 (MQ=60)
                                                                                                                  AAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCC                                   >  SRR19973080.2558167/1‑150 (MQ=60)
                                                                                                                  AAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCC                                   >  SRR19973080.2165209/1‑150 (MQ=60)
                                                                                                                     ggtGTTCGCGAATGCGCTTGGTGATTATATCTTGCTCACGCATCGGTCCCTGCTTGTGTTTGAGGTGTGGGTTTTTGACTTCCACGATCTTCATCCTCTTTCTCCTTTGAGGGGCCGCCAAAAAAAAAATCGACGGATTATACCTCCTTT                                <  SRR19973080.3919948/147‑1 (MQ=60)
                                                                                                                       AGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCCCGCATCAGTCCCTGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCT                              <  SRR19973080.4132132/150‑1 (MQ=60)
                                                                                                                         ttggctaacGCGCTTGTTGCTTATATTTTGCTCACGCATCAGTCCCAGCTTGTTTTTGACGAGTGGTTTTTTGACTTCCACTATCTACATACTGTTTCTCCTTTGCGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTC                            <  SRR19973080.1593254/141‑1 (MQ=60)
                                                                                                                         TTCGCGAACGCGCTTGGTGCTGATATTTTGCACCCGCACCCGTCCCAGCTTGTTTTTGACGAGGGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGTGGGGCCCCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTC                            <  SRR19973080.1593975/150‑1 (MQ=60)
                                                                                                                          TCGCTAAAGCGCTTGGTGATTATATATTGCACACGCATCAGTCCCTGATTGTTTTTGAGGTGTGGGTGTTTTACTTCCACTATCTTCATACTCTTTCTCCTTTGTGGGGCAGCCAAAAAAAAAATCGACGGATTATACCTCCTTTCTTCA                           <  SRR19973080.465501/150‑1 (MQ=60)
                                                                                                                            GCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGTGTGGGTTTTTGACTTCCACTATCTTCATACTCTTTCTCCTTTGTGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAG                         <  SRR19973080.1353000/150‑1 (MQ=60)
                                                                                                                             CGAAAGCGCTTGGTGCTGATATTTTGCTCACGCATCTGTCCCTGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGTGGGGCAGCCAAAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGG                        <  SRR19973080.1431017/150‑1 (MQ=60)
                                                                                                                              GAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGC                       >  SRR19973080.1700831/1‑150 (MQ=60)
                                                                                                                              GAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGC                       >  SRR19973080.1613741/1‑150 (MQ=60)
                                                                                                                               AAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCG                      >  SRR19973080.3139440/1‑150 (MQ=60)
                                                                                                                                 cgcgaTTGGTTCTGATATCTTGCTCACGCATCTGTCCCAGTTTGTGTTTGACGTGTGGGTTTTTGACTTCCACGATATTCATAATCTTTCTCCTTTGAGGGGCAGCCAAAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGC                    <  SRR19973080.223636/145‑1 (MQ=60)
                                                                                                                                 TGCGCTTGGTGCTTATATGTTGCTCCCGCATCAGTCCCTGCTGGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTCCTCCTTTGTGGGGCAGCAAAAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGC                    <  SRR19973080.223591/150‑1 (MQ=60)
                                                                                                                                 AGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGC                    >  SRR19973080.4182833/1‑150 (MQ=60)
                                                                                                                                 AGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGC                    >  SRR19973080.4182597/1‑150 (MQ=60)
                                                                                                                                  GCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCA                   >  SRR19973080.1079550/1‑150 (MQ=60)
                                                                                                                                  GCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCA                   >  SRR19973080.1080074/1‑150 (MQ=60)
                                                                                                                                   CGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAA                  >  SRR19973080.4285244/1‑150 (MQ=60)
                                                                                                                                      TTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATAT               >  SRR19973080.1599038/1‑150 (MQ=60)
                                                                                                                                        GGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTC             >  SRR19973080.3083718/1‑150 (MQ=60)
                                                                                                                                          TGCTGATATATTGCTCACGAATCAGTCCCAGCTTGTTTTTGACGTGTGGGTGTTTGACTTCCACTATCTTAATACTCTTTCGCCTTTGAGGGGCACCCAAAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTT           <  SRR19973080.3148945/150‑1 (MQ=60)
                                                                                                                                           GCTGATATATTGCTCACGCATCAGTCCCAGCTTGTTTTTGACTTGTGGGTGTTTGACTTCCACGATCTTCATACTATTTCTCCTTTGCGGGGCAGCAACAAAAAAACTCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTT          <  SRR19973080.420577/150‑1 (MQ=60)
                                                                                                                                           GCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTT          >  SRR19973080.1087521/1‑150 (MQ=60)
                                                                                                                                            CTTATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGAATTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTT         <  SRR19973080.2615606/150‑1 (MQ=60)
                                                                                                                                            CTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGTGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTT         <  SRR19973080.371397/150‑1 (MQ=60)
                                                                                                                                            CTGATATATTGCACACGCATCAGTCCCAGTTTGTGTTTGACGCGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCGCCTTTGAGGGGCAGCCAAAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTT         <  SRR19973080.1693412/150‑1 (MQ=60)
                                                                                                                                                 ATATTGCTCACGCATCAGTCCCAGCTTGTGTTGGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCAACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTG    <  SRR19973080.2152016/150‑1 (MQ=60)
                                                                                                                                                   ATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGAC  >  SRR19973080.3594692/1‑150 (MQ=60)
                                                                                                                                                   ATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGAC  >  SRR19973080.3594731/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
TTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGAC  >  CP009273/2614021‑2614317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑