Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F98 I0 R1 799 294.1 9752082 92.2% 8991419 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,614,168 C→A 71.5% D22Y (GAT→TAT)  upp ← uracil phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,614,1680CA71.5% 314.6 / 167.0 210D22Y (GAT→TAT) uppuracil phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (34/26);  new base A (74/69);  total (108/102)
Fisher's exact test for biased strand distribution p-value = 5.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 215 total aligned reads displayed.

AATTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGAC  >  CP009273/2614019‑2614317
                                                                                                                                                     |                                                                                                                                                     
aaTTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATc                                                                                                                                                       <  2:3033518/150‑1 (MQ=255)
   tttCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTg                                                                                                                                                    >  2:3877280/1‑150 (MQ=255)
    ttCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGc                                                                                                                                                   <  2:3949627/150‑1 (MQ=255)
      cTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTc                                                                                                                                                 <  2:2216901/150‑1 (MQ=255)
       ttACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCa                                                                                                                                                <  1:1953378/150‑1 (MQ=255)
       ttACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAACTCAGCACGCTACCCACATCGGAAGCGAGTTAGCGAAAGCGCTTGGTGCTGATATATTGCTCa                                                                                                                                                >  1:4769060/1‑150 (MQ=255)
       ttACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCGTAGATAGTTAATTTTTACGTTTCGTGGTCGGCGGTCGCTTAGTAAGTAAGCAGGCTAACCACATAGGAAGCGAGTTAGCGAAATCGCTTTGTGGTGATATATTTCtaa                                                                                                                                                >  1:1354300/1‑148 (MQ=255)
              tGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCa                                                                                                                                         <  1:737284/150‑1 (MQ=255)
              tGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACATCGGAAGCGAGTTCGCGAAAGCGCTTTGTGCTGATATCTTGCTCACGCATCa                                                                                                                                         >  2:75096/1‑150 (MQ=255)
                aTCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGt                                                                                                                                       >  2:4689511/1‑150 (MQ=255)
                aTCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTAATTTTTACGTTTAGAGGTCGGCGGTCGCTTAGTAAGTAAGCAGGCTAACCACATAGGAAGCGAGTTAGCGAAAGCGCTTTGTGCTGATATATTTCTAACCCAACAGt                                                                                                                                       >  1:3577504/1‑150 (MQ=255)
                  cTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTAGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTcc                                                                                                                                     >  2:4298221/1‑150 (MQ=255)
                   tGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTccc                                                                                                                                    >  2:3401644/1‑150 (MQ=255)
                     gTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACATCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAg                                                                                                                                  >  1:2262623/1‑150 (MQ=255)
                            tCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTgtgt                                                                                                                           <  1:2941770/150‑1 (MQ=255)
                              tACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGttt                                                                                                                         <  2:425779/150‑1 (MQ=255)
                              tACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGttt                                                                                                                         <  1:4358263/150‑1 (MQ=255)
                                 cGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGAc                                                                                                                      >  2:694164/1‑150 (MQ=255)
                                 cGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTAGTAATTCAGCAGGCTAACCACATCGGAAGCGAGTTAGCGAAATCGCTTGGTGCTTATATATTGATCAACCATCAGTACCACCtttttttttaa                                                                                                                      >  1:4582120/1‑147 (MQ=255)
                                  gggCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACg                                                                                                                     >  2:3980005/1‑150 (MQ=255)
                                  gggCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACg                                                                                                                     >  1:3227815/1‑150 (MQ=255)
                                    gCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAg                                                                                                                   <  2:3122073/150‑1 (MQ=255)
                                     ccGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGt                                                                                                                  <  1:3865984/150‑1 (MQ=255)
                                      cGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTg                                                                                                                 >  2:4641799/1‑150 (MQ=255)
                                      cGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTg                                                                                                                 >  2:4643472/1‑150 (MQ=255)
                                          ccAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGgtg                                                                                                             <  2:4249223/150‑1 (MQ=255)
                                            aGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGtt                                                                                                           >  2:2868681/1‑150 (MQ=255)
                                             gCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGttt                                                                                                          <  1:1135865/150‑1 (MQ=255)
                                             gCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGttt                                                                                                          >  1:3855166/1‑150 (MQ=255)
                                             gCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGttt                                                                                                          <  2:2404468/150‑1 (MQ=255)
                                             gCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGttt                                                                                                          <  1:2046957/150‑1 (MQ=255)
                                              ccTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTg                                                                                                         <  1:4167074/150‑1 (MQ=255)
                                              ccTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTg                                                                                                         >  2:4538986/1‑150 (MQ=255)
                                                  cGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACtt                                                                                                     <  2:1537755/150‑1 (MQ=255)
                                                   gATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTg                                                                                                         >  2:569600/1‑145 (MQ=255)
                                                   gATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTg                                                                                                         <  1:569671/145‑1 (MQ=255)
                                                    aTAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTcc                                                                                                   >  1:3591235/1‑150 (MQ=255)
                                                      aGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGTCGAGTGGGTGTTTGACTTCCAc                                                                                                 >  1:3265260/1‑150 (MQ=255)
                                                      aGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCAc                                                                                                 >  1:314494/1‑150 (MQ=255)
                                                       gTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACg                                                                                                <  1:1607448/150‑1 (MQ=255)
                                                       gTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACg                                                                                                <  1:1607966/150‑1 (MQ=255)
                                                        ttACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGa                                                                                               >  1:425462/1‑150 (MQ=255)
                                                         tACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGAt                                                                                              <  2:1724241/150‑1 (MQ=255)
                                                           cTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCt                                                                                            <  2:1520449/150‑1 (MQ=255)
                                                           cTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCt                                                                                            <  2:3687372/150‑1 (MQ=255)
                                                           cTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCt                                                                                            <  2:3590904/150‑1 (MQ=255)
                                                              tttCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCa                                                                                         <  1:2601642/150‑1 (MQ=255)
                                                               ttCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCAt                                                                                        <  1:2173698/150‑1 (MQ=255)
                                                               ttCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCAt                                                                                        >  2:4555384/1‑150 (MQ=255)
                                                                tCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATa                                                                                       <  2:3434987/150‑1 (MQ=255)
                                                                tCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACATCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATa                                                                                       >  1:3872557/1‑150 (MQ=255)
                                                                  cGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATAct                                                                                     <  1:75730/150‑1 (MQ=255)
                                                                   gTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGACTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATActc                                                                                    >  1:4619421/1‑150 (MQ=255)
                                                                       cGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTtc                                                                                <  1:3279520/150‑1 (MQ=255)
                                                                        gAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTtct                                                                               >  2:620700/1‑150 (MQ=255)
                                                                           gTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCt                                                                            <  2:1792067/150‑1 (MQ=255)
                                                                           gTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCt                                                                            <  2:4769866/150‑1 (MQ=255)
                                                                               gcggTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGa                                                                        <  1:689744/150‑1 (MQ=255)
                                                                                 ggTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAgg                                                                      >  2:4035837/1‑150 (MQ=255)
                                                                                 ggTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAgg                                                                      >  2:1138484/1‑150 (MQ=255)
                                                                                  gTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAggg                                                                     <  2:4743601/150‑1 (MQ=255)
                                                                                   tCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAgggg                                                                    <  1:1572478/150‑1 (MQ=255)
                                                                                   tCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAgggg                                                                    <  1:38465/150‑1 (MQ=255)
                                                                                   tCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAgggg                                                                    <  1:2463749/150‑1 (MQ=255)
                                                                                        tCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGcc                                                               <  2:1696662/150‑1 (MQ=255)
                                                                                        tCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGcc                                                               <  1:4645483/150‑1 (MQ=255)
                                                                                        tCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGcc                                                               >  1:2961592/1‑150 (MQ=255)
                                                                                          gTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCcac                                                             >  2:770910/1‑150 (MQ=255)
                                                                                           tAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCcaca                                                            >  1:815230/1‑150 (MQ=255)
                                                                                             aGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACaaa                                                          >  2:3126719/1‑150 (MQ=255)
                                                                                              gTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACaaaa                                                         <  2:2460485/150‑1 (MQ=255)
                                                                                                cagcagGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACaaaaaa                                                       >  1:3744158/1‑150 (MQ=255)
                                                                                                cagcagGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACaaaaaa                                                       >  1:704525/1‑150 (MQ=255)
                                                                                                   cagGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAAt                                                    >  2:3487741/1‑150 (MQ=255)
                                                                                                   cagGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAAt                                                    >  2:4184147/1‑150 (MQ=255)
                                                                                                   cagGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAAt                                                    <  1:2903645/150‑1 (MQ=255)
                                                                                                    agGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATc                                                   <  1:3937725/150‑1 (MQ=255)
                                                                                                     ggCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCg                                                  <  1:3411121/150‑1 (MQ=255)
                                                                                                        tACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACg                                               >  1:1027801/1‑150 (MQ=255)
                                                                                                         aCCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACgg                                              >  1:3694915/1‑150 (MQ=255)
                                                                                                          cccACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGa                                             <  2:132657/150‑1 (MQ=255)
                                                                                                            cACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTTATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGTGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGAtt                                           <  2:2578092/150‑1 (MQ=255)
                                                                                                            cACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGAtt                                           >  1:196714/1‑150 (MQ=255)
                                                                                                             aCTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGAATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTTACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATta                                          <  1:3169707/150‑1 (MQ=255)
                                                                                                                    aaGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATAcctcc                                   >  2:297856/1‑150 (MQ=255)
                                                                                                                    aaGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATAcctcc                                   >  1:2484623/1‑150 (MQ=255)
                                                                                                                    aaGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATAcctcc                                   >  1:52277/1‑150 (MQ=255)
                                                                                                                     aGCGAGTTCGCTAACGCGATTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGATTGTTTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATAcctcct                                  <  2:4582120/150‑1 (MQ=255)
                                                                                                                       ctaGTTCGCTAACGGGTTTGGTGCTTATATTTTGCTCACGCATCTGTCCCTGTTTTTTTTTGACGCGTGGTTTTTTTACTTCCACGATATTCATACTATTTCTCATTTGTGGGGCACCAAAAAAAAAAATCGACGGATTATACCTCCttt                                <  1:2954448/148‑1 (MQ=255)
                                                                                                                        gAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTttc                               >  2:2748792/1‑150 (MQ=255)
                                                                                                                         aGTTCGCGAAAGCGGTTGGTGCTGATATATTGCACACGCATCAGTCCCAGCTTGTGTTTGACGAGGGGGTTTTTGACTTCCACTATCTTCATACTCTTACTCCTTTGTGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTttct                              <  1:3099060/150‑1 (MQ=255)
                                                                                                                          gTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTttctt                             <  2:4047310/150‑1 (MQ=255)
                                                                                                                           ttCGCGAACGCGCTGGGTGCTGATATATTGCTCCCGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTAATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTttcttc                            <  2:1232790/150‑1 (MQ=255)
                                                                                                                             cgcgAAAGCGCTTGGTGCTGTTATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCaa                          <  2:2312784/150‑1 (MQ=255)
                                                                                                                             cgcgAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCaa                          <  2:4857874/150‑1 (MQ=255)
                                                                                                                             cgcgAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCaa                          >  2:4199178/1‑150 (MQ=255)
                                                                                                                                     cgcTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCac                  >  2:1900180/1‑149 (MQ=255)
                                                                                                                                           ttgattatatatTGCTCACGAATAAGTCCCTGTTTGTGTTTGACGTGTGGGTTTTTTACTACCACTATCTTCATACTCTTTCTCCTTTGAGGGGCAGCAAAAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCt            <  1:4555173/144‑1 (MQ=255)
                                                                                                                                               tGATATATTGCTCCCGCAACAGTCCCAGCTTGTTTTTGACGAGTGGGTGTTTGACTTCCACTATCTTCATAATCTTTCACCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTc        <  2:890958/150‑1 (MQ=255)
                                                                                                                                                 atatatTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGt      >  2:3156710/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AATTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGAC  >  CP009273/2614019‑2614317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AATTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGAC  >  CP009273/2614019‑2614317
                                                                                                                                                     |                                                                                                                                                     
AATTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATA                                                                                                                                                       <  SRR19973081.2317365/150‑1 (MQ=60)
 ATTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATAT                                                                                                                                                      <  SRR19973081.3850974/150‑1 (MQ=60)
  TTTTCTTACCTTTGTTCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATT                                                                                                                                                     <  SRR19973081.4155940/150‑1 (MQ=60)
  TTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATATTTACTTTTTAAGTTTAGAGGTAGGCGGTAGCTTCGTAATTAAGCAAGCAAACCACATCAGAAGCCAGTTCTCGAAAGCGCATGGTTCTGATATATT                                                                                                                                                     >  SRR19973081.1094661/1‑150 (MQ=56)
       TTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCA                                                                                                                                                <  SRR19973081.1965726/150‑1 (MQ=60)
       TTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAACTCAGCACGCTACCCACATCGGAAGCGAGTTAGCGAAAGCGCTTGGTGCTGATATATTGCTCA                                                                                                                                                >  SRR19973081.4797881/1‑150 (MQ=60)
              TGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCA                                                                                                                                         <  SRR19973081.742116/150‑1 (MQ=60)
                 TCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACATCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTC                                                                                                                                      >  SRR19973081.320586/1‑150 (MQ=60)
                     GTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACATCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAG                                                                                                                                  >  SRR19973081.2276917/1‑150 (MQ=60)
                        GATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTT                                                                                                                               >  SRR19973081.2369463/1‑150 (MQ=60)
                            TCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGT                                                                                                                           <  SRR19973081.2959946/150‑1 (MQ=60)
                            TCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGT                                                                                                                           <  SRR19973081.2959908/150‑1 (MQ=60)
                             CTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTT                                                                                                                          <  SRR19973081.3282312/150‑1 (MQ=60)
                              TACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTT                                                                                                                         <  SRR19973081.4384668/150‑1 (MQ=60)
                              TACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTT                                                                                                                         <  SRR19973081.4384660/150‑1 (MQ=60)
                                CCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGA                                                                                                                       <  SRR19973081.714580/150‑1 (MQ=60)
                                 CGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACATCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGAC                                                                                                                      >  SRR19973081.4388809/1‑150 (MQ=60)
                                 CGGGCCGTTCCAGCCTTCGATAGTTACTTTTTACGTTTCGAGGTCGGCGGTCGCTTCGTAATTCAGCAGGCTACCCACATAGGAACCCAGTTAGCGAAAGCGCTTTGTTCTGATATATTTCTCAAGCAACAGTCCCAccatgttttttaa                                                                                                                      >  SRR19973081.2598903/1‑137 (MQ=60)
                                  GGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACG                                                                                                                     >  SRR19973081.3247284/1‑150 (MQ=60)
                                     CCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGT                                                                                                                  <  SRR19973081.3889424/150‑1 (MQ=60)
                                     CCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGT                                                                                                                  <  SRR19973081.3889757/150‑1 (MQ=60)
                                             GCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTT                                                                                                          <  SRR19973081.3910674/150‑1 (MQ=60)
                                             GCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTT                                                                                                          >  SRR19973081.3878857/1‑150 (MQ=60)
                                             GCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTT                                                                                                          >  SRR19973081.3878676/1‑150 (MQ=60)
                                             GCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTT                                                                                                          <  SRR19973081.1143258/150‑1 (MQ=60)
                                             GCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTT                                                                                                          <  SRR19973081.77936/150‑1 (MQ=60)
                                             GCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTT                                                                                                          <  SRR19973081.2059846/150‑1 (MQ=60)
                                              gatctGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTG                                                                                                         <  SRR19973081.573725/145‑1 (MQ=60)
                                              gatctGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTG                                                                                                         <  SRR19973081.573654/145‑1 (MQ=60)
                                              CCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTG                                                                                                         <  SRR19973081.1143777/150‑1 (MQ=60)
                                              CCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTG                                                                                                         <  SRR19973081.4192551/150‑1 (MQ=60)
                                              CCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTG                                                                                                         <  SRR19973081.1583299/150‑1 (MQ=60)
                                                  CGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTT                                                                                                     <  SRR19973081.4873059/150‑1 (MQ=60)
                                                    ATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCC                                                                                                   >  SRR19973081.3709777/1‑150 (MQ=60)
                                                    ATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCC                                                                                                   >  SRR19973081.1974856/1‑150 (MQ=60)
                                                    ATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCC                                                                                                   >  SRR19973081.3612704/1‑150 (MQ=60)
                                                    ATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCC                                                                                                   >  SRR19973081.3613035/1‑150 (MQ=60)
                                                    ATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCAACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTTCTCACGCAGCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCC                                                                                                   >  SRR19973081.1974311/1‑150 (MQ=60)
                                                      AGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGTCGAGTGGGTGTTTGACTTCCAC                                                                                                 >  SRR19973081.3285000/1‑150 (MQ=60)
                                                      AGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCAC                                                                                                 >  SRR19973081.316105/1‑150 (MQ=60)
                                                      AGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCAC                                                                                                 >  SRR19973081.316794/1‑150 (MQ=60)
                                                       GTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACG                                                                                                <  SRR19973081.1617782/150‑1 (MQ=60)
                                                       GTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACG                                                                                                <  SRR19973081.1618306/150‑1 (MQ=60)
                                                       GTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACG                                                                                                <  SRR19973081.1617817/150‑1 (MQ=60)
                                                       GTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACG                                                                                                <  SRR19973081.1618304/150‑1 (MQ=60)
                                                        TTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGA                                                                                               >  SRR19973081.428291/1‑150 (MQ=60)
                                                           CTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCT                                                                                            >  SRR19973081.3445724/1‑150 (MQ=60)
                                                            TTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTT                                                                                           <  SRR19973081.2625038/150‑1 (MQ=60)
                                                              TTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCA                                                                                         <  SRR19973081.2617850/150‑1 (MQ=60)
                                                              TTTCCGTTTAGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCA                                                                                         <  SRR19973081.3179626/150‑1 (MQ=60)
                                                               GTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCAT                                                                                        <  SRR19973081.2187531/150‑1 (MQ=60)
                                                                TCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATA                                                                                       >  SRR19973081.3896366/1‑150 (MQ=60)
                                                                TCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATA                                                                                       >  SRR19973081.1421025/1‑150 (MQ=60)
                                                                TCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACATCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATA                                                                                       >  SRR19973081.3896369/1‑150 (MQ=60)
                                                                  CGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACT                                                                                     <  SRR19973081.76575/150‑1 (MQ=60)
                                                                  CGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACT                                                                                     <  SRR19973081.76512/150‑1 (MQ=60)
                                                                  CGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACT                                                                                     <  SRR19973081.76447/150‑1 (MQ=60)
                                                                   GTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGACTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTC                                                                                    >  SRR19973081.4647491/1‑150 (MQ=60)
                                                                   GTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGACTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTC                                                                                    >  SRR19973081.4647465/1‑150 (MQ=60)
                                                                       CGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTC                                                                                <  SRR19973081.3299449/150‑1 (MQ=60)
                                                                             CGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTT                                                                          >  SRR19973081.2704726/1‑150 (MQ=60)
                                                                               GCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGA                                                                        <  SRR19973081.694378/150‑1 (MQ=60)
                                                                               GCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGA                                                                        <  SRR19973081.4660818/150‑1 (MQ=60)
                                                                                   TCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGG                                                                    <  SRR19973081.4055790/150‑1 (MQ=60)
                                                                                   TCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGG                                                                    <  SRR19973081.1582634/150‑1 (MQ=60)
                                                                                   TCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGG                                                                    <  SRR19973081.2479022/150‑1 (MQ=60)
                                                                                   TCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGG                                                                    <  SRR19973081.38922/150‑1 (MQ=60)
                                                                                        TCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCC                                                               >  SRR19973081.2979850/1‑150 (MQ=60)
                                                                                        TCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCC                                                               <  SRR19973081.4673687/150‑1 (MQ=60)
                                                                                           TAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACA                                                            >  SRR19973081.820311/1‑150 (MQ=60)
                                                                                           TAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACA                                                            <  SRR19973081.3110354/150‑1 (MQ=60)
                                                                                                CAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAA                                                       >  SRR19973081.3767083/1‑150 (MQ=60)
                                                                                                CAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAA                                                       >  SRR19973081.709221/1‑150 (MQ=60)
                                                                                                CAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAA                                                       >  SRR19973081.709201/1‑150 (MQ=60)
                                                                                                   CAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAAT                                                    <  SRR19973081.2921520/150‑1 (MQ=60)
                                                                                                    AGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATC                                                   <  SRR19973081.3961861/150‑1 (MQ=60)
                                                                                                     GGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCG                                                  <  SRR19973081.3432095/150‑1 (MQ=60)
                                                                                                     GGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCG                                                  <  SRR19973081.3183810/150‑1 (MQ=60)
                                                                                                        TACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACG                                               >  SRR19973081.1034731/1‑150 (MQ=60)
                                                                                                         ACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGG                                              >  SRR19973081.3717402/1‑150 (MQ=60)
                                                                                                            CACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATT                                           >  SRR19973081.198420/1‑150 (MQ=60)
                                                                                                            CACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATT                                           >  SRR19973081.2073603/1‑150 (MQ=60)
                                                                                                             ACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGAATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTTACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTA                                          <  SRR19973081.3188995/150‑1 (MQ=60)
                                                                                                                   GAAGCGAGTTGGCGAAAGCGATTGGTGCTTATATTTTGCTCACGCATCTGTCCCAGCTTGTGTTTGACGTGTGGGTGTTTGACTTCCACGATCTTAATACTCTTTCTCATTTGAGGGGCAGCCAAAAAAAAAATCGACGGATTATACCTC                                    <  SRR19973081.340373/150‑1 (MQ=60)
                                                                                                                    AAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCC                                   >  SRR19973081.199918/1‑150 (MQ=60)
                                                                                                                    AAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCC                                   >  SRR19973081.2500051/1‑150 (MQ=60)
                                                                                                                    AAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCC                                   >  SRR19973081.52889/1‑150 (MQ=60)
                                                                                                                    AAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCC                                   >  SRR19973081.2500076/1‑150 (MQ=60)
                                                                                                                       ctagttcgctaacgggtTTGGTGCTTATATTTTGCTCACGCATCTGTCCCTGTTTTTTTTTGACGCGTGGTTTTTTTACTTCCACGATATTCATACTATTTCTCATTTGTGGGGCACCAAAAAAAAAAATCGACGGATTATACCTCCTTT                                <  SRR19973081.2972667/133‑1 (MQ=30)
                                                                                                                       CGTGTTCGCTAAAGCGCTTGGTGCTTATATTTGGCCCACCCATCAGTCCCTGCTTGTTTTTGAGGTGGGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGCCACCAAAAAAAAAAATCGACGGATTATACCTCCTTT                                <  SRR19973081.2938619/150‑1 (MQ=60)
                                                                                                                         AGTTCGCGAAAGCGGTTGGTGCTGATATATTGCACACGCATCAGTCCCAGCTTGTGTTTGACGAGGGGGTTTTTGACTTCCACTATCTTCATACTCTTACTCCTTTGTGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCT                              <  SRR19973081.3118068/150‑1 (MQ=60)
                                                                                                                                   AGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGC                    >  SRR19973081.3793373/1‑150 (MQ=60)
                                                                                                                                      GCTTGGTGATGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGTGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAAT                 <  SRR19973081.3626614/150‑1 (MQ=60)
                                                                                                                                        TTGGTGCTGATATCTTGCTCACGCATCAGTCCCTGCTTGTGTTTGACGTGTGGGTGTTTGACTTCCACGATCTTAATACTCTTTCTCCTTTGTGGGGCAGCCAAAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATAT               <  SRR19973081.682107/150‑1 (MQ=60)
                                                                                                                                           GTGCTTATATATTGCTCACGAATCTGTCCCTGCTTTTGTTTGACTTGTGGTTTTTTTACTTCCACTATCTTCATACTCTTTCTCCTTTGTGGGGCAGCCAAAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCT            <  SRR19973081.4583003/150‑1 (MQ=60)
                                                                                                                                             GCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTT          >  SRR19973081.3744916/1‑150 (MQ=60)
                                                                                                                                                GATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCG       >  SRR19973081.3318395/1‑150 (MQ=60)
                                                                                                                                                 ATATTTTGCTCACGCATCAGTCCCAGCTTGTGTTTGCCGTGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGCGGGGCAGCCAAAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGT      <  SRR19973081.4233530/150‑1 (MQ=60)
                                                                                                                                                   ATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGTGTGGGTGTTTGACTTCCACTATCTTCATACTCTTTCTCCTTTGTGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTG    <  SRR19973081.2204816/150‑1 (MQ=60)
                                                                                                                                                    TATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGTGTGGGTTTTTTACTTCCACTTTCTTCATAATATTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGA   <  SRR19973081.120287/150‑1 (MQ=60)
                                                                                                                                                     GTTGCTCACGCTTCAGTCCCTGTTTGTTTTTGACGTGTGGGTGTTTGACTTCCAGTATATTCATAATTTTTCTCATTTGTGGGGCATCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGAC  <  SRR19973081.4684405/150‑1 (MQ=60)
                                                                                                                                                     CTTGCTCACGAATCAGTCCCAGCTTGTGTTTTACGAGTGGGTTTTTGACTTCCACGATATTCATACTCTTACCCCTTTGTGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGAC  <  SRR19973081.4685031/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
AATTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGAC  >  CP009273/2614019‑2614317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑