Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,614,168 C→A 100% D22Y (GAT→TAT)  upp ← uracil phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,614,1680CA100.0% 506.8 / ‑6.7 162D22Y (GAT→TAT) uppuracil phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (84/75);  minor base T (0/3);  total (84/78)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 166 total aligned reads displayed.

TTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTC  >  CP009273/2614023‑2614311
                                                                                                                                                 |                                                                                                                                               
ttCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTAATTTTTACGTTTCGATGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACATCGGAAGCCAGTTAGCGAAAGCGCTTGGTTCTGATATAtt                                                                                                                                               >  1:730496/1‑148 (MQ=255)
  cTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGc                                                                                                                                             <  1:3214678/148‑1 (MQ=255)
         ttGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTTCTAACGCa                                                                                                                                      >  2:2589112/1‑148 (MQ=255)
         ttGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCa                                                                                                                                      <  1:2593145/148‑1 (MQ=255)
          tGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCAt                                                                                                                                     <  2:2053563/148‑1 (MQ=255)
            aTCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCa                                                                                                                                   >  2:177070/1‑148 (MQ=255)
             tCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAg                                                                                                                                  >  2:1337822/1‑148 (MQ=255)
             tCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTAATTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCATGGTGCTGATATATTTCTCAAGCAACAg                                                                                                                                  >  2:3081179/1‑148 (MQ=255)
              cTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGt                                                                                                                                 <  1:1222530/148‑1 (MQ=255)
                ggTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTcc                                                                                                                               >  2:18955/1‑148 (MQ=255)
                 gTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTccc                                                                                                                              <  1:2290210/148‑1 (MQ=255)
                 gTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTccc                                                                                                                              >  2:376256/1‑148 (MQ=255)
                      tttCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCtt                                                                                                                         >  2:2981440/1‑148 (MQ=255)
                      tttCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCtt                                                                                                                         >  2:2980868/1‑148 (MQ=255)
                      tttCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCtt                                                                                                                         >  2:1151151/1‑148 (MQ=255)
                        tCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCCCTTCCGAAGCGAGTTCGCGAAAGCGCTTGGTGGTGATATATTGCTCACGCATCAGTCCCAGCTtgg                                                                                                                       >  1:3190441/1‑147 (MQ=255)
                        tCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTtgt                                                                                                                       >  2:184018/1‑148 (MQ=255)
                        tCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCGACCCACATCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCCGCTtgt                                                                                                                       >  1:3190477/1‑148 (MQ=255)
                         cTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTtgtg                                                                                                                      <  1:1343862/148‑1 (MQ=255)
                           aCCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGtt                                                                                                                    <  2:87827/148‑1 (MQ=255)
                           aCCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGtt                                                                                                                    <  2:1181251/148‑1 (MQ=255)
                             cGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTg                                                                                                                  >  2:2645471/1‑148 (MQ=255)
                             cGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACATCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTg                                                                                                                  >  2:168454/1‑148 (MQ=255)
                               ggCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGAc                                                                                                                >  2:1409648/1‑148 (MQ=255)
                                gCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACg                                                                                                               <  2:2505215/148‑1 (MQ=255)
                                gCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACg                                                                                                               >  2:2611100/1‑148 (MQ=255)
                                gCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACg                                                                                                               >  2:1543855/1‑148 (MQ=255)
                                      ccAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTggg                                                                                                         >  2:1817268/1‑148 (MQ=255)
                                         gCCTTCGAGAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGgtgt                                                                                                      <  2:2286745/148‑1 (MQ=255)
                                          ccTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGtt                                                                                                     >  2:3053696/1‑148 (MQ=255)
                                           cTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCGTGGTGCTGATATATTGCTCACGCATCAGTCCCCGCTTGTGTTTGACGAGTGGGTGttt                                                                                                    >  1:1477850/1‑148 (MQ=255)
                                               gATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACt                                                                                                <  2:1440800/148‑1 (MQ=255)
                                                 tAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTTCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTc                                                                                              >  1:2828131/1‑148 (MQ=255)
                                                 tAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTc                                                                                              >  2:289048/1‑148 (MQ=255)
                                                    ttACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCAc                                                                                           >  2:1717890/1‑148 (MQ=255)
                                                       cTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGAt                                                                                        <  1:935393/148‑1 (MQ=255)
                                                       cTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGAt                                                                                        <  1:2205193/148‑1 (MQ=255)
                                                        tttttCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATc                                                                                       >  2:1428049/1‑148 (MQ=255)
                                                           ttCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTc                                                                                    <  2:421308/148‑1 (MQ=255)
                                                           ttCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTc                                                                                    <  2:421163/148‑1 (MQ=255)
                                                                 ttCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATActc                                                                              >  1:3008769/1‑148 (MQ=255)
                                                                 ttCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATActc                                                                              >  1:1010520/1‑148 (MQ=255)
                                                                 ttCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATActc                                                                              >  2:1238270/1‑148 (MQ=255)
                                                                 ttCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGGGGGTGTTTGACTTCCACGATCTTCATActc                                                                              >  2:280415/1‑148 (MQ=255)
                                                                  tCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATActct                                                                             <  1:524217/148‑1 (MQ=255)
                                                                   cGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCtt                                                                            >  1:1263464/1‑148 (MQ=255)
                                                                   cGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCtt                                                                            <  2:3100910/148‑1 (MQ=255)
                                                                   cGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCGTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCtt                                                                            >  1:1263484/1‑148 (MQ=255)
                                                                      ggTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTtct                                                                         >  1:2017768/1‑148 (MQ=255)
                                                                      ggTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTtct                                                                         <  1:2028663/148‑1 (MQ=255)
                                                                        tCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTcc                                                                       <  1:3297012/148‑1 (MQ=255)
                                                                         cggcggTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCt                                                                      <  2:3021752/148‑1 (MQ=255)
                                                                            cggTCGCTTCGTAAGTCAGCAGGCGACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATGGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTg                                                                   <  2:560474/148‑1 (MQ=255)
                                                                              gTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAg                                                                 <  2:213449/148‑1 (MQ=255)
                                                                               tCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAgg                                                                >  2:729957/1‑148 (MQ=255)
                                                                                  cTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGc                                                             >  1:1137112/1‑148 (MQ=255)
                                                                                  cTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGc                                                             >  1:1137882/1‑148 (MQ=255)
                                                                                    tCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAg                                                           >  2:2482672/1‑148 (MQ=255)
                                                                                    tCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAg                                                           >  2:1676599/1‑148 (MQ=255)
                                                                                     cGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGc                                                          <  2:2352850/148‑1 (MQ=255)
                                                                                           tCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACaaa                                                    >  2:1055104/1‑148 (MQ=255)
                                                                                               cagGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACaaaaaaa                                                <  1:1355639/148‑1 (MQ=255)
                                                                                               cagGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACaaaaaaa                                                >  2:12842/1‑148 (MQ=255)
                                                                                                 ggCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAAt                                              <  2:1570240/148‑1 (MQ=255)
                                                                                                   cTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCg                                            >  2:728601/1‑148 (MQ=255)
                                                                                                   cTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCg                                            <  1:2741994/148‑1 (MQ=255)
                                                                                                     aCCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTGGAGGGGCAGCCACAAAAAAAATCGAc                                          <  2:725498/148‑1 (MQ=255)
                                                                                                       ccACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACgg                                        >  1:1717918/1‑148 (MQ=255)
                                                                                                        cACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGa                                       <  2:1933829/148‑1 (MQ=255)
                                                                                                        cACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGa                                       <  2:263793/148‑1 (MQ=255)
                                                                                                          cTTCGGACGCGAGTTCGCGAACGCGCTGGGGGCTGATATATTGCACACGCAGCCGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTGGAGGGGCAGCCACAAAAAAAATCGACGGAtt                                     <  2:2266465/148‑1 (MQ=255)
                                                                                                           ttCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTTATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATta                                    <  2:1036398/148‑1 (MQ=255)
                                                                                                           ttCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATta                                    >  2:3306021/1‑148 (MQ=255)
                                                                                                                aaGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATAcct                               >  1:127542/1‑148 (MQ=255)
                                                                                                                  gCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATAcctcc                             >  1:3155985/1‑148 (MQ=255)
                                                                                                                   cGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATAcctcct                            <  2:1027777/148‑1 (MQ=255)
                                                                                                                     aGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCttt                          >  2:2504407/1‑148 (MQ=255)
                                                                                                                      gTTCGCGAAAGCGCTTGGTGCTTATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGTCACAAAAAAAATCGACGGATTATACCTCCTttc                         <  2:2113770/148‑1 (MQ=255)
                                                                                                                      gTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTttc                         >  1:1612029/1‑148 (MQ=255)
                                                                                                                      gTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTttc                         >  1:1611977/1‑148 (MQ=255)
                                                                                                                      gTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTttc                         <  2:1325567/148‑1 (MQ=255)
                                                                                                                          gcgAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCa                     <  2:782683/148‑1 (MQ=255)
                                                                                                                          gcgAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCa                     <  2:2395301/148‑1 (MQ=255)
                                                                                                                             aaaGCGCTTGGTGCTGATATATTGCTCACGCATCCGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAgg                  <  2:619257/148‑1 (MQ=255)
                                                                                                                               tgcgaTTGGTGCTTATATATTGCTCACGCATCAGTCCAAGCTTGTGTTTGACTTGTGGGTTTTTGACTTCCACTATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAAAAAAAAACTCGACGGATTATACCTCCTTTCTTCAAggcg                <  1:2589112/147‑1 (MQ=255)
                                                                                                                                gcgcTTGGTGCTTATATATTGCTCACGAATCAGTCCCAGCTTGTGTTTGACGAGTGGTTTTTTGACTTCCACGATATTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAggcgg               <  1:2184898/148‑1 (MQ=255)
                                                                                                                                gcgcTTGGTGCTGATATTTTGCTCACGCATCAGTCCCAGCTTGTGTTGGACGAGTGGGTGTTTGACTTCAACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAggcgg               <  1:1894377/148‑1 (MQ=255)
                                                                                                                                gcgcTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAggcgg               >  2:2930342/1‑148 (MQ=255)
                                                                                                                                gcgcTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAggcgg               >  1:2514869/1‑148 (MQ=255)
                                                                                                                                gcgcTTGGTGCTGATATATTGCTCACGAATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAAAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAggcgg               <  1:1894119/148‑1 (MQ=255)
                                                                                                                                   cTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCaa            <  2:2413062/148‑1 (MQ=255)
                                                                                                                                   cTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCaa            <  2:2412024/148‑1 (MQ=255)
                                                                                                                                   cTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCaa            <  2:197312/148‑1 (MQ=255)
                                                                                                                                   cTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCaa            >  1:804804/1‑148 (MQ=255)
                                                                                                                                    ttGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTAATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAat           <  2:339254/148‑1 (MQ=255)
                                                                                                                                      ggTGCTGATATTTTGCTCACCAATCAGTCCCAGTTTGTTTTTGCCGTGTGGGTTTTTGACTTCCACTATCTTCATACTCTTTCTCCTTTGTGGGGCAGCAAAAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAatat         <  1:959096/148‑1 (MQ=255)
                                                                                                                                           tGATATATTGCTCACGCATCTGTCCCAGTTTTTGTTTTACGAGTGGGTGTTTGAATTCCTATATCTTCATACTCTTTCTCCTTTGTGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCttt    <  1:1036604/148‑1 (MQ=255)
                                                                                                                                            gATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCtttt   <  1:3306021/148‑1 (MQ=255)
                                                                                                                                             atatatTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTc  >  1:2395566/1‑148 (MQ=255)
                                                                                                                                             atatatTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTc  >  1:590680/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
TTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTC  >  CP009273/2614023‑2614311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AATTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTG  >  CP009273/2614019‑2614315
                                                                                                                                                     |                                                                                                                                                   
AATTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTAATTTTTACGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACATCGGAAGCCAGTTAGCGAAAGCGCTTTGTGCTGATATA                                                                                                                                                     >  SRR19973070.1043720/1‑150 (MQ=60)
  TTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATT                                                                                                                                                   <  SRR19973070.2150295/150‑1 (MQ=60)
  TTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATT                                                                                                                                                   <  SRR19973070.833392/150‑1 (MQ=60)
  TTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTAATTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTAATCAGGATACCCACTTCGGAATCGAGTTCGCGAAAGCGCTTGGTGCTGATATATT                                                                                                                                                   <  SRR19973070.834075/150‑1 (MQ=60)
  TTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTAATTTTTACGTTTCGATGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACATCGGAAGCCAGTTAGCGAAAGCGCTTGGTTCTGATATATT                                                                                                                                                   >  SRR19973070.735676/1‑150 (MQ=60)
     TCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCT                                                                                                                                                <  SRR19973070.1187453/150‑1 (MQ=60)
      CTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTC                                                                                                                                               <  SRR19973070.3235467/150‑1 (MQ=60)
      CTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTC                                                                                                                                               <  SRR19973070.1587980/150‑1 (MQ=60)
          CCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCGTCGATAGTTAATTTTTCCGTTTAGAGGTCGGCGGTCGCGTCGTAAATAAGCAGGCTACCCCCATCGGAAACGAGTTCGCGAAACCGCTTGGTGCTGATATAATTCTCACGC                                                                                                                                           >  SRR19973070.1251927/1‑150 (MQ=60)
            TTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCAT                                                                                                                                         <  SRR19973070.3233953/150‑1 (MQ=60)
             TTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATC                                                                                                                                        <  SRR19973070.2610092/150‑1 (MQ=60)
               GATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAG                                                                                                                                      >  SRR19973070.1918233/1‑150 (MQ=60)
                ATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGT                                                                                                                                     <  SRR19973070.318866/150‑1 (MQ=60)
                  CTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCC                                                                                                                                   <  SRR19973070.1231089/150‑1 (MQ=60)
                     GTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAG                                                                                                                                <  SRR19973070.2305436/150‑1 (MQ=60)
                        GATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTAGCGAAAGCGCTTGGTGCTGATATATTTCTCACGCATCAGTCCCAGCTT                                                                                                                             >  SRR19973070.163553/1‑150 (MQ=60)
                          TTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCCCTTCCGAAGCGAGTTCGCGAAAGCGCTTGGTGGTGATATATTGCTCACGCATCAGTCCCAGCTTGG                                                                                                                           >  SRR19973070.3211084/1‑150 (MQ=60)
                          TTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCGACCCACATCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCCGCTTGT                                                                                                                           >  SRR19973070.3211120/1‑150 (MQ=60)
                             CTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTT                                                                                                                        <  SRR19973070.1353085/150‑1 (MQ=60)
                              TACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTT                                                                                                                       <  SRR19973070.2519647/150‑1 (MQ=60)
                                    GCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAG                                                                                                                 <  SRR19973070.88517/150‑1 (MQ=60)
                                        TTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGG                                                                                                             >  SRR19973070.583441/1‑150 (MQ=60)
                                             GCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCGTGGTGCTGATATATTGCTCACGCATCAGTCCCCGCTTGTGTTTGACGAGTGGGTGTTT                                                                                                        >  SRR19973070.1488193/1‑150 (MQ=60)
                                                TTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGAC                                                                                                     <  SRR19973070.1879643/150‑1 (MQ=60)
                                                   GATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTTCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTC                                                                                                  >  SRR19973070.2846430/1‑150 (MQ=60)
                                                     TAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCA                                                                                                <  SRR19973070.1336428/150‑1 (MQ=60)
                                                          ACTATTTCCGTTTCGAGGTCGGCGGTCGCTTCTTAATTCAGAAGCCTAACAACTTCGCAAGCGAGTTCCCCAAAGCGCATGGTGCTCATAAATTGCTCACGCATCAGacccaccgtgagatagacgagtgggtgtatgaaaacaacgctc                                                                                           >  SRR19973070.1759572/1‑107 (MQ=27)
                                                           CTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCT                                                                                          <  SRR19973070.942063/150‑1 (MQ=60)
                                                           CTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCT                                                                                          <  SRR19973070.2219716/150‑1 (MQ=60)
                                                            TTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTT                                                                                         <  SRR19973070.3080633/150‑1 (MQ=60)
                                                                   GTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTC                                                                                  >  SRR19973070.3027982/1‑150 (MQ=60)
                                                                   GTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTC                                                                                  >  SRR19973070.1017708/1‑150 (MQ=60)
                                                                     TTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTT                                                                                >  SRR19973070.1272344/1‑150 (MQ=60)
                                                                     TTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTT                                                                                >  SRR19973070.1272314/1‑150 (MQ=60)
                                                                     TTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCGTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTT                                                                                >  SRR19973070.1272334/1‑150 (MQ=60)
                                                                      TCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTT                                                                               <  SRR19973070.527772/150‑1 (MQ=60)
                                                                       CGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTC                                                                              >  SRR19973070.746767/1‑150 (MQ=60)
                                                                       CGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTC                                                                              <  SRR19973070.2563314/150‑1 (MQ=60)
                                                                        GAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCT                                                                             >  SRR19973070.2031422/1‑150 (MQ=60)
                                                                         AGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTC                                                                            >  SRR19973070.3236917/1‑150 (MQ=60)
                                                                          GGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCC                                                                           <  SRR19973070.2042374/150‑1 (MQ=60)
                                                                          GGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCC                                                                           <  SRR19973070.2042381/150‑1 (MQ=60)
                                                                            TCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTT                                                                         <  SRR19973070.3318142/150‑1 (MQ=60)
                                                                                   TCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGG                                                                  <  SRR19973070.487806/150‑1 (MQ=60)
                                                                                    CGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGC                                                                 >  SRR19973070.1144994/1‑150 (MQ=60)
                                                                                    CGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGC                                                                 >  SRR19973070.1145771/1‑150 (MQ=60)
                                                                                                   CAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAAT                                                  <  SRR19973070.1364906/150‑1 (MQ=60)
                                                                                                       CTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGAC                                              <  SRR19973070.2759631/150‑1 (MQ=60)
                                                                                                        TACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACG                                             >  SRR19973070.1480537/1‑150 (MQ=60)
                                                                                                        TACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTAACGCATCAGTCCCAGCTTGTGTTTGACGAGGGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCATTGGAGGGGCAGCCACAAAAAAAATCGACG                                             >  SRR19973070.2282468/1‑150 (MQ=60)
                                                                                                         ACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGG                                            >  SRR19973070.1729795/1‑150 (MQ=60)
                                                                                                              CTTCGGATGCGATTTCGCGAAAGCGCTTGGTGCTTATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGTGTGGGTTTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTAT                                       <  SRR19973070.2218762/150‑1 (MQ=60)
                                                                                                                  GGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCT                                   >  SRR19973070.128662/1‑150 (MQ=60)
                                                                                                                    AAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCC                                 >  SRR19973070.3176384/1‑150 (MQ=60)
                                                                                                                        GAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTC                             >  SRR19973070.1623208/1‑150 (MQ=60)
                                                                                                                        GAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTC                             >  SRR19973070.1623260/1‑150 (MQ=60)
                                                                                                                                  AAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGG                   >  SRR19973070.2531253/1‑150 (MQ=60)
                                                                                                                                   tgcgaTTGGTGCTTATATATTGCTCACGCATCAGTCCAAGCTTGTGTTTGACTTGTGGGTTTTTGACTTCCACTATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAAAAAAAAACTCGACGGATTATACCTCCTTTCTTCAAGGCGGC                  <  SRR19973070.2606030/145‑1 (MQ=60)
                                                                                                                                    GCGCTTGGTGCTTATATATTGCTCACGAATCAGTCCCAGCTTGTGTTTGACGAGTGGTTTTTTGACTTCCACGATATTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCA                 <  SRR19973070.2199346/150‑1 (MQ=60)
                                                                                                                                    GCGCTTGGTGCTGATATTTTGCTCACGCATCAGTCCCAGCTTGTGTTGGACGAGTGGGTGTTTGACTTCAACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCA                 <  SRR19973070.1907218/150‑1 (MQ=60)
                                                                                                                                    GCGCTTGGTGCTGATATATTGCTCACGAATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAAAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCA                 <  SRR19973070.1906958/150‑1 (MQ=60)
                                                                                                                                     CGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAA                >  SRR19973070.810339/1‑150 (MQ=60)
                                                                                                                                         TGGTGTTTATATTTTGCTCACGAATCAGTCCCTGTTTGTTTTTGCCGTGTGGGTTTTTGACTTCCACTATGTTCATACTCTTTCTCGTTTGTGGGGCACCAAAAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATT            <  SRR19973070.2798558/150‑1 (MQ=60)
                                                                                                                                          GGTGCTGATATTTTGCTCACCAATCAGTCCCAGTTTGTTTTTGCCGTGTGGGTTTTTGACTTCCACTATCTTCATACTCTTTCTCCTTTGTGGGGCAGCAAAAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTC           <  SRR19973070.965939/150‑1 (MQ=60)
                                                                                                                                               TGATATATTGCTCACGCATCTGTCCCAGTTTTTGTTTTACGAGTGGGTGTTTGAATTCCTATATCTTCATACTCTTTCTCCTTTGTGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTC      <  SRR19973070.1043927/150‑1 (MQ=60)
                                                                                                                                               TGATATATTGCTCACGCATCAGTCCCAGGTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTC      <  SRR19973070.2707603/150‑1 (MQ=60)
                                                                                                                                               TGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTC      >  SRR19973070.594763/1‑150 (MQ=60)
                                                                                                                                               TGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTC      >  SRR19973070.2411379/1‑150 (MQ=60)
                                                                                                                                               TGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTC      >  SRR19973070.1075184/1‑150 (MQ=60)
                                                                                                                                                GATATATTGCTCCCGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCG     <  SRR19973070.3327183/150‑1 (MQ=60)
                                                                                                                                                GATATATTGCTCACGCATCAGTCCAAGCTTGTTTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCATTTGAGGGGCAGCAACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCG     <  SRR19973070.3327608/150‑1 (MQ=60)
                                                                                                                                                 ATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGT    >  SRR19973070.2669071/1‑150 (MQ=60)
                                                                                                                                                  TATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTT   >  SRR19973070.2426255/1‑150 (MQ=60)
                                                                                                                                                  TATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTT   >  SRR19973070.2426241/1‑150 (MQ=60)
                                                                                                                                                   ATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTG  >  SRR19973070.86041/1‑150 (MQ=60)
                                                                                                                                                   ATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTG  >  SRR19973070.1773402/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
AATTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTG  >  CP009273/2614019‑2614315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑