Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,614,168 C→A 100% D22Y (GAT→TAT)  upp ← uracil phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,614,1680CA99.2% 642.7 / ‑5.6 211D22Y (GAT→TAT) uppuracil phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/2);  major base A (105/97);  minor base T (0/7);  total (105/106)
Fisher's exact test for biased strand distribution p-value = 6.80e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 213 total aligned reads displayed.

TTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTT  >  CP009273/2614023‑2614309
                                                                                                                                                 |                                                                                                                                              
ttCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATAtt                                                                                                                                              >  2:3219062/1‑148 (MQ=255)
 tCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTCAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTg                                                                                                                                             >  2:1803109/1‑148 (MQ=255)
  cTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCGTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTAAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGc                                                                                                                                            >  2:78032/1‑148 (MQ=255)
   ttACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCt                                                                                                                                           >  1:2917419/1‑148 (MQ=255)
   ttACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCt                                                                                                                                           <  2:3156164/148‑1 (MQ=255)
   ttACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCt                                                                                                                                           <  2:2997035/148‑1 (MQ=255)
    tACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTAGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTc                                                                                                                                          >  2:363802/1‑148 (MQ=255)
     aCCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTGCGTTTAGAGGTCGGCGGTCGCTTCGTAAGTCAGCACGCTAACCACATCGGAAGCCACTTTGCGAAACCCCATTGTGGTGTTATATTGCTCa                                                                                                                                         >  1:980368/1‑148 (MQ=255)
      ccTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCAc                                                                                                                                        >  2:851742/1‑148 (MQ=255)
        tttGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGc                                                                                                                                      <  1:2440603/148‑1 (MQ=255)
        tttGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGc                                                                                                                                      <  1:678734/148‑1 (MQ=255)
           gATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTACGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACATCGGAAGCGAGTTAGCGAAAGCGCATTGTGCTGATATATTGCTCaaccaac                                                                                                                                   >  1:1658101/1‑142 (MQ=255)
            aTCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCa                                                                                                                                  >  1:3495061/1‑148 (MQ=255)
              cTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGt                                                                                                                                <  2:2696432/148‑1 (MQ=255)
                ggTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTcc                                                                                                                              <  2:823647/148‑1 (MQ=255)
                  tCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCa                                                                                                                            >  2:2424745/1‑148 (MQ=255)
                     aTTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTTCTAACGCAACAGTCCCAGCt                                                                                                                         >  1:2532795/1‑148 (MQ=255)
                          tACTGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTgtgt                                                                                                                    <  2:2106795/148‑1 (MQ=255)
                           aCCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGtt                                                                                                                   <  2:3199998/148‑1 (MQ=255)
                               ggCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTg                                                                                                                 >  1:2224595/1‑146 (MQ=255)
                               ggCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTg                                                                                                                 <  2:2224595/146‑1 (MQ=255)
                                   gTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGt                                                                                                           <  1:3326110/148‑1 (MQ=255)
                                   gTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGt                                                                                                           <  1:1941203/148‑1 (MQ=255)
                                    ttCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTg                                                                                                          >  1:1098512/1‑148 (MQ=255)
                                    ttCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTg                                                                                                          >  1:3181242/1‑148 (MQ=255)
                                     tCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTgg                                                                                                         >  1:1029695/1‑148 (MQ=255)
                                      ccAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTTCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTggg                                                                                                        >  1:3399340/1‑148 (MQ=255)
                                      ccAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATAAGTCCCAGCTTGTGTTTGACTAGTggg                                                                                                        >  1:2001231/1‑148 (MQ=255)
                                         gCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGgtgt                                                                                                     >  1:3455231/1‑148 (MQ=255)
                                         gCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGAAGAGTGGgtgt                                                                                                     >  2:2572433/1‑148 (MQ=255)
                                          ccTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGtt                                                                                                    >  1:609387/1‑148 (MQ=255)
                                           cTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGttt                                                                                                   >  2:3250754/1‑148 (MQ=255)
                                            ttCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTg                                                                                                  >  1:556655/1‑148 (MQ=255)
                                            ttCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTg                                                                                                  >  2:332673/1‑148 (MQ=255)
                                                aTAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACtt                                                                                              <  1:2488609/148‑1 (MQ=255)
                                                aTAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACtt                                                                                              >  1:2372392/1‑148 (MQ=255)
                                                 tAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTc                                                                                             >  2:2749371/1‑148 (MQ=255)
                                                 tAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTc                                                                                             >  1:1347922/1‑148 (MQ=255)
                                                  aGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTcc                                                                                            <  2:578771/148‑1 (MQ=255)
                                                   gTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCa                                                                                           <  2:2882215/148‑1 (MQ=255)
                                                   gTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCa                                                                                           <  1:316491/148‑1 (MQ=255)
                                                    ttACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCAc                                                                                          >  2:695545/1‑148 (MQ=255)
                                                    ttACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCAc                                                                                          >  2:1839738/1‑148 (MQ=255)
                                                      aCTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGa                                                                                        <  1:710236/148‑1 (MQ=255)
                                                       cTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGAt                                                                                       <  2:3092492/148‑1 (MQ=255)
                                                             ccGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCAt                                                                                 <  1:2995586/148‑1 (MQ=255)
                                                              cGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATa                                                                                <  1:405579/148‑1 (MQ=255)
                                                              cGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATa                                                                                <  1:895400/148‑1 (MQ=255)
                                                              cGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATa                                                                                <  2:68475/148‑1 (MQ=255)
                                                              cGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATa                                                                                <  1:3319524/148‑1 (MQ=255)
                                                                      ggTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGGTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTtct                                                                        >  1:1206851/1‑148 (MQ=255)
                                                                      ggTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTtct                                                                        >  2:2661395/1‑148 (MQ=255)
                                                                             ggTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGa                                                                 <  2:1870472/148‑1 (MQ=255)
                                                                               tCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAgg                                                               <  2:3296510/148‑1 (MQ=255)
                                                                                     cGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGc                                                         >  1:2415100/1‑148 (MQ=255)
                                                                                       tAAGTCAGCAGGCTACCCACTTCGAAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCca                                                       >  2:1961690/1‑148 (MQ=255)
                                                                                          gTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑aa                                                   >  1:2648432/1‑148 (MQ=255)
                                                                                          gTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑aa                                                   <  1:1998578/148‑1 (MQ=255)
                                                                                          gTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑aa                                                   >  1:2600892/1‑148 (MQ=255)
                                                                                               cagGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑aaaaaaa                                              >  1:2078053/1‑148 (MQ=255)
                                                                                               cagGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑aaaaaaa                                              >  1:3301045/1‑148 (MQ=255)
                                                                                               cagGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑aaaaaaa                                              >  1:2509815/1‑148 (MQ=255)
                                                                                               cagGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑aaaaaaa                                              <  1:2795393/148‑1 (MQ=255)
                                                                                                 ggCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAAt                                            >  2:2139126/1‑148 (MQ=255)
                                                                                                 ggCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAAt                                            <  1:78032/148‑1 (MQ=255)
                                                                                                  gCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATc                                           >  1:1697331/1‑148 (MQ=255)
                                                                                                     aCCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATCGAc                                        >  1:1141073/1‑148 (MQ=255)
                                                                                                      cccACTTCGGAAGCGCGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAAATCGAc                                        >  2:2938620/1‑148 (MQ=255)
                                                                                                      cccACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCAACAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATCGACg                                       <  2:3278098/148‑1 (MQ=255)
                                                                                                       ccACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATCGACgg                                      >  2:1048530/1‑148 (MQ=255)
                                                                                                       ccACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATCGACgg                                      >  2:1368913/1‑148 (MQ=255)
                                                                                                        cACTTCGGAAGCTTGTTCGCGAAAGCGCTTGGTGCTTATATATTGCTCACGCATCAGTCCCAGTTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATATTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATCGACGGa                                     <  1:2029029/148‑1 (MQ=255)
                                                                                                         aCTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATCGACGGAt                                    >  1:3074874/1‑148 (MQ=255)
                                                                                                           ttCGGAAGCGAGTTCGCGAACGCGGTTGGTGCTTATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTTTTTGACTTCCACTATCTTAATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATCGACGGATta                                  <  2:1206851/148‑1 (MQ=255)
                                                                                                           ttCGGAAGCGAGTTCGCGAACGCGCTTGGTGATTATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACTATCTTAATACTCTTTCGCCTTTGAGGGGCAGCCAA‑AAAAAAAATCGACGGATta                                  <  1:1394721/148‑1 (MQ=255)
                                                                                                              ggAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATGGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATCGACGGATTATAc                               <  2:143693/148‑1 (MQ=255)
                                                                                                              ggAAGCGAGTTCGCGAAAGCGCTGGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATCGACGGATTATAc                               <  2:1440836/148‑1 (MQ=255)
                                                                                                                 aGCGAGTTCGCGAAAGCGCTTGGTGCTTATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATCGACGGATTATAcctc                            <  2:2008892/148‑1 (MQ=255)
                                                                                                                   cGAGTTCGCGAACGCGCTGGGTGCTGATATAGTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATCGACGGATTATAcctcct                          <  2:1218300/148‑1 (MQ=255)
                                                                                                                   cGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGCTCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATCGACGGATTATAcctcct                          <  2:1073857/148‑1 (MQ=255)
                                                                                                                   cGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATCGACGGATTATAcctcct                          <  2:3498669/148‑1 (MQ=255)
                                                                                                                   cGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATCGACGGATTATAcctcct                          >  2:1335321/1‑148 (MQ=255)
                                                                                                                   cGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATCGACGGATTATAcctcct                          >  2:1880935/1‑148 (MQ=255)
                                                                                                                   cGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATCGACGGATTATAcctcct                          <  2:1651960/148‑1 (MQ=255)
                                                                                                                     aGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATCGACGGATTATACCTCCttt                        >  1:472681/1‑148 (MQ=255)
                                                                                                                      gTTCGCGAAAGCGCTTGGTGCTGATATTTTGCTCACGCATCAGTCCCAGCTTGTGTTTGAGGAGTGGGTGTTTGACTTCCACGATCTTAATACTCTTTCTCCTTTTAGGGGCAGCCAC‑AAAAAAAATCGACGGATTATACCTCCTttc                       <  2:453574/148‑1 (MQ=255)
                                                                                                                           cgAAAGGGTTTGGTGGTTATATATTGATCACGAATCTGTCCCAGCTTGTGTTTGACGTGTGGTTTTTTGACTTCCACTATATTCATACTCTTTCTCCTTTGAGGGGCAGCCAA‑AAAAAAAATCGACGGATTATACCTCCTTTCTTCaa                  <  1:1839738/148‑1 (MQ=255)
                                                                                                                              aaGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATCGACGGATTATACCTCCTTTCTTCAAggc               >  2:521607/1‑148 (MQ=255)
                                                                                                                                gcgcTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATCGACGGATTATACCTCCTTTCTTCAAggcgg             >  1:1318308/1‑148 (MQ=255)
                                                                                                                                  ggttGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATAGACTGATTATACCTCCTTTCTTCAAGGCGGCa           <  2:3142873/146‑1 (MQ=255)
                                                                                                                                  gcTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGATTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCa           <  2:1767501/148‑1 (MQ=255)
                                                                                                                                    ttgttgCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGTGTGGGTGTTTGAATTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAA‑AAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAat         <  2:674919/148‑1 (MQ=255)
                                                                                                                                      ggTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAatat       <  2:523101/148‑1 (MQ=255)
                                                                                                                                        tGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTc     >  1:1238539/1‑148 (MQ=255)
                                                                                                                                        ggCTGATATATTGCTCACGCATCAGTCCCTGCTTGTGTTTGACGTGTGGTTTTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGCGGGGCCGCCAC‑AAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTc     <  1:2424745/147‑1 (MQ=255)
                                                                                                                                          cttatatatTCCTCACGAATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATATTCATACTCTTTCTCCTTTGAGGGGCACCAAA‑AAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCtt   <  1:2179044/145‑1 (MQ=255)
                                                                                                                                          cTGATATCTTGCACCCGCATCAGTCCCAGCTTGTGTTTGCCGAGTGGGTGTTTGACGTCCACGATCTTCATCCCCTTTCGCCTTTGAGGGGCAGCCAC‑AAAACAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCtt   <  2:1507404/148‑1 (MQ=255)
                                                                                                                                          cTGATATATTGCTCACGCATCCGTCCCAGCTTGTGTTTGACGTGTGGGTGTTTGACTTCCACTATCTTCATACTCTTTCTCATTTGAGGGGCAGCCAC‑AAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCtt   <  1:2754977/148‑1 (MQ=255)
                                                                                                                                           ttttatTTTGCTCACGCATCAGTCCCAGGTTGTTTTTGACGTGTGGTTGTTTGACTTCCACGATCTTCATACTATTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCttt  <  1:332673/145‑1 (MQ=255)
                                                                                                                                           tGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCttt  <  2:2320140/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
TTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTT  >  CP009273/2614023‑2614309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGA  >  CP009273/2614020‑2614316
                                                                                                                                                    |                                                                                                                                                    
ATTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTGCAGCCGTCGATAGTTAATTTTTACGTGTATATGTCGGCGGTCGCTTCGTAAATAAGCAGGCTACCCACATAGGAATCGAGTTCGCGTAAGCGCTTGGTGGTGATATAT                                                                                                                                                     >  SRR19973065.1443817/1‑150 (MQ=60)
    TCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCT                                                                                                                                                 <  SRR19973065.2631828/150‑1 (MQ=60)
    TCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCT                                                                                                                                                 >  SRR19973065.2935163/1‑150 (MQ=60)
    TCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTAGCGAAAGCGCTTGGTGCTGATATATTTCT                                                                                                                                                 >  SRR19973065.1172238/1‑150 (MQ=60)
       TACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCGTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCAC                                                                                                                                              >  SRR19973065.2026799/1‑150 (MQ=60)
           TTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCAT                                                                                                                                          <  SRR19973065.2455987/150‑1 (MQ=60)
           TTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCAT                                                                                                                                          <  SRR19973065.683257/150‑1 (MQ=60)
           TTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCAT                                                                                                                                          <  SRR19973065.1233551/150‑1 (MQ=60)
            TTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTACGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACATCGGAAGCGAGTTAGCGAAAGCGCATTGTGCTGATATATTGCTCAaccaac                                                                                                                                         >  SRR19973065.1669209/1‑144 (MQ=60)
             TGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCA                                                                                                                                        >  SRR19973065.3516306/1‑150 (MQ=60)
                   GGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCA                                                                                                                                  <  SRR19973065.1392983/150‑1 (MQ=60)
                      CGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTTCTAACGCAACAGTCCCAGCT                                                                                                                               >  SRR19973065.2548690/1‑150 (MQ=60)
                      CGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCT                                                                                                                               >  SRR19973065.2869578/1‑150 (MQ=60)
                                CGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAATTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGAC                                                                                                                     >  SRR19973065.2238776/1‑150 (MQ=60)
                                     CGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTG                                                                                                                >  SRR19973065.1105739/1‑150 (MQ=60)
                                     CGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTG                                                                                                                >  SRR19973065.3200463/1‑150 (MQ=60)
                                      GTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGG                                                                                                               <  SRR19973065.1953460/150‑1 (MQ=60)
                                      GTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGG                                                                                                               >  SRR19973065.1036514/1‑150 (MQ=60)
                                      GTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGG                                                                                                               <  SRR19973065.1953900/150‑1 (MQ=60)
                                      GTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGG                                                                                                               <  SRR19973065.3346404/150‑1 (MQ=60)
                                      GTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGG                                                                                                               <  SRR19973065.3346411/150‑1 (MQ=60)
                                      GGTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGG                                                                                                               <  SRR19973065.2528360/150‑1 (MQ=60)
                                       TTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTTCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGG                                                                                                              >  SRR19973065.3420119/1‑150 (MQ=60)
                                       TTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGG                                                                                                              >  SRR19973065.2014122/1‑150 (MQ=60)
                                       TTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATAAGTCCCAGCTTGTGTTTGACTAGTGGG                                                                                                              >  SRR19973065.2014182/1‑150 (MQ=60)
                                          CAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTTACGAGTGGGTGT                                                                                                           >  SRR19973065.3476278/1‑150 (MQ=60)
                                          CAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGT                                                                                                           >  SRR19973065.3476290/1‑150 (MQ=60)
                                          CAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGT                                                                                                           <  SRR19973065.2176223/150‑1 (MQ=60)
                                          CAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGT                                                                                                           >  SRR19973065.1800693/1‑150 (MQ=60)
                                          CAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGT                                                                                                           >  SRR19973065.448921/1‑150 (MQ=60)
                                           AGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTT                                                                                                          >  SRR19973065.613262/1‑150 (MQ=60)
                                             CCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTG                                                                                                        >  SRR19973065.560209/1‑150 (MQ=60)
                                             CCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACATCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATAGCTCACGCATCAGTCCCAGCGTGTGTTTGACGAGTGGGTGTTTT                                                                                                        >  SRR19973065.559954/1‑150 (MQ=60)
                                               TTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGAC                                                                                                      <  SRR19973065.3154920/150‑1 (MQ=60)
                                                 CGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTT                                                                                                    >  SRR19973065.2387201/1‑150 (MQ=60)
                                                 CGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTT                                                                                                    >  SRR19973065.2386943/1‑150 (MQ=60)
                                                 CGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTT                                                                                                    >  SRR19973065.985547/1‑150 (MQ=60)
                                                  GATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTC                                                                                                   >  SRR19973065.1356838/1‑150 (MQ=60)
                                                   ATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCC                                                                                                  <  SRR19973065.2504269/150‑1 (MQ=60)
                                                   ATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCC                                                                                                  <  SRR19973065.2503277/150‑1 (MQ=60)
                                                      GTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACG                                                                                               <  SRR19973065.318649/150‑1 (MQ=60)
                                                         ACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATC                                                                                            <  SRR19973065.714983/150‑1 (MQ=60)
                                                         ACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATC                                                                                            <  SRR19973065.715036/150‑1 (MQ=60)
                                                                CCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATAC                                                                                     <  SRR19973065.3013930/150‑1 (MQ=60)
                                                                 CGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACT                                                                                    <  SRR19973065.408419/150‑1 (MQ=60)
                                                                 CGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACT                                                                                    <  SRR19973065.2667921/150‑1 (MQ=60)
                                                                 CGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACT                                                                                    <  SRR19973065.3339774/150‑1 (MQ=60)
                                                                 CGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACT                                                                                    <  SRR19973065.901108/150‑1 (MQ=60)
                                                                 CGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACT                                                                                    <  SRR19973065.3339725/150‑1 (MQ=60)
                                                                     TCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTT                                                                                <  SRR19973065.741007/150‑1 (MQ=60)
                                                                       GAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGGTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCT                                                                              >  SRR19973065.1214616/1‑150 (MQ=60)
                                                                           TCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTT                                                                          <  SRR19973065.2226463/150‑1 (MQ=60)
                                                                                   CGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGC                                                                  <  SRR19973065.2068864/150‑1 (MQ=60)
                                                                                      TTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGC                                                               >  SRR19973065.2430095/1‑150 (MQ=60)
                                                                                      TTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGC                                                               >  SRR19973065.2430794/1‑150 (MQ=60)
                                                                                      TTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGC                                                               >  SRR19973065.2430315/1‑150 (MQ=60)
                                                                                         GTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC                                                            >  SRR19973065.3451133/1‑150 (MQ=60)
                                                                                           AAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAA                                                          >  SRR19973065.2617044/1‑150 (MQ=60)
                                                                                           AAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAA                                                          >  SRR19973065.2664759/1‑150 (MQ=60)
                                                                                             GTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAA                                                        <  SRR19973065.2011519/150‑1 (MQ=60)
                                                                                                AGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAA                                                     >  SRR19973065.2525769/1‑150 (MQ=60)
                                                                                                AGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAA                                                     >  SRR19973065.2525588/1‑150 (MQ=60)
                                                                                                AGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAA                                                     >  SRR19973065.2091340/1‑150 (MQ=60)
                                                                                                AGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAA                                                     >  SRR19973065.3321149/1‑150 (MQ=60)
                                                                                                AGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAA                                                     >  SRR19973065.3321077/1‑150 (MQ=60)
                                                                                                  CAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAAT                                                   <  SRR19973065.2812713/150‑1 (MQ=60)
                                                                                                  CAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAAT                                                   >  SRR19973065.2508501/1‑150 (MQ=60)
                                                                                                   AGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATC                                                  >  SRR19973065.1708381/1‑150 (MQ=60)
                                                                                                   AGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATC                                                  >  SRR19973065.1098705/1‑150 (MQ=60)
                                                                                                   AGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATC                                                  >  SRR19973065.1708656/1‑150 (MQ=60)
                                                                                                    GGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCG                                                 <  SRR19973065.78819/150‑1 (MQ=60)
                                                                                                    GGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCG                                                 <  SRR19973065.700173/150‑1 (MQ=60)
                                                                                                      CTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGAC                                               >  SRR19973065.1148450/1‑150 (MQ=60)
                                                                                                       TACCCACTGCGGATGCGTTTTCGCGAATGCGGTTGGTGCTGATTTATTGCTCACGAATCTGTCCCAGGTTGTGTTTGAGGAGTGGTTTTTTGACTTCAACTATATTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACG                                              <  SRR19973065.1129173/150‑1 (MQ=60)
                                                                                                          CCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGAT                                           >  SRR19973065.3093660/1‑150 (MQ=60)
                                                                                                          CCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGAT                                           >  SRR19973065.3044368/1‑150 (MQ=60)
                                                                                                          CCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGAT                                           >  SRR19973065.145741/1‑150 (MQ=60)
                                                                                                           CACTTCGGAAGCTTGTTCGCGAAAGCGCTTGGTGCTTATATATTGCTCACGCATCAGTCCCAGTTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATATTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATT                                          <  SRR19973065.2042058/150‑1 (MQ=60)
                                                                                                           CACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATT                                          >  SRR19973065.1470270/1‑150 (MQ=60)
                                                                                                              TTCGGAAGCGAGTTCGCGAACGCGCTTGGTGATTATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACTATCTTAATACTCTTTCGCCTTTGAGGGGCAGCCAAAAAAAAAATCGACGGATTATA                                       <  SRR19973065.1403877/150‑1 (MQ=60)
                                                                                                                ggtAAGCGAGTTGGCGAAAGCGCTTGTTGCTGATATATTGCTCACGCATCTTTCCCAGATTGTGTTTGAGGAGTGGGTTTTTGAATTCCAGTATCTTAATACTCTTTCTCCTTTGTGGGGCAGCCACAAAAAAAATCGACGGATTATACC                                     <  SRR19973065.545993/147‑1 (MQ=60)
                                                                                                                      CGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTT                               >  SRR19973065.3082615/1‑150 (MQ=60)
                                                                                                                      CGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTT                               >  SRR19973065.1355416/1‑150 (MQ=60)
                                                                                                                      CGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTT                               >  SRR19973065.475914/1‑150 (MQ=60)
                                                                                                                        AGTTCGCTAACGCGCTTGGTGCTTATATATTGCTCACCAATCAGTCCCAGCTTGTGTTTGACGTGTGGGTTTTTTACTTCCACGTTCTTAATAATCTTTCTCCTTTGAGGGGCACCCAAAAAAAAAATCGACGGATTATACCTCCTTTCT                             <  SRR19973065.360283/150‑1 (MQ=60)
                                                                                                                         GTTCGCGAAAGCGCTTGGTGCTTATTTATTGCTCACGCATCAGTCCCAGGTTGTGTTTGCCGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTT                            <  SRR19973065.1956481/150‑1 (MQ=60)
                                                                                                                          TTCGCGAAAGCGGTTGGTGCTGATATTTGGCTCACGAATCAGTCCCCGCTTGTGTTTTACGTGTGGGTGTTTGACTTCCACTATTTTCATACTGTTTCTCCTTTGTGGGGCAGCCAAAAAAAAAATCGACGGATTATACCTCCTTTCTTC                           <  SRR19973065.2982032/150‑1 (MQ=60)
                                                                                                                           TCGCTAAAGCGCTTGGTGCTTATATTTTGCACACGCATCAGTCCCTGCTTGTTTTTGACGTGTGGTTTTTTTACTTCCACGTTCTTCATAATCTTTCTCCTTTGTGGGGCAGCCAAAAAAAAAATCGACGGATTATACCTCCTTTCTTCA                          <  SRR19973065.659960/150‑1 (MQ=60)
                                                                                                                              CGAACGCGCTTGGTGCTGATATTTTGCTCACGAATCTGTCCCAGTTTGTTTTTGACGTGTGGGTTTTTGACTCCCACTATATTAATACTCTTTCTCCTTTGTGGGGCAGCCAAAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGG                       <  SRR19973065.60615/150‑1 (MQ=60)
                                                                                                                              CGAAAGGGTTTGGTGGTTATATATTGATCACGAATCTGTCCCAGCTTGTGTTTGACGTGTGGTTTTTTGACTTCCACTATATTCATACTCTTTCTCCTTTGAGGGGCAGCCAAAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGG                       <  SRR19973065.1851872/150‑1 (MQ=60)
                                                                                                                              CGAAAGCGATTGGTGCTGATTTTTTGCTCACGAATCAGTCCCTGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCCGCCAAAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGG                       <  SRR19973065.803223/150‑1 (MQ=60)
                                                                                                                                 AAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGG                    >  SRR19973065.1327028/1‑150 (MQ=60)
                                                                                                                                  AGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGC                   >  SRR19973065.3404110/1‑150 (MQ=60)
                                                                                                                                     GCTTGGTGCTTATATATTGCTCACGCATCAGTCCCAGGTTGTGTTTGAGGAGTGGTTGTTTGACTTCCACTATCTTCATACTCTTTCTCCTTTGTGGGGCAGCAACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAAT                <  SRR19973065.2580937/150‑1 (MQ=60)
                                                                                                                                         GGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTTAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTC            >  SRR19973065.3503599/1‑150 (MQ=60)
                                                                                                                                         GGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTC            >  SRR19973065.1246668/1‑150 (MQ=60)
                                                                                                                                           TGCTTATTTCTTTCTCACGCATCAGTCCCAGTTTGTGTTTGACGTTTGGGTGTTTTACTTCCACTATTTTAATACTCTTTCTCCTTTGAGGGGCAGCCAAAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTT          <  SRR19973065.2441181/150‑1 (MQ=60)
                                                                                                                                           GGCTGATATATTGCTCACGCATCAGTCCCTGCTTGTGTTTGACGTGTGGTTTTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGCGGGGCCGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTT          <  SRR19973065.2440041/150‑1 (MQ=60)
                                                                                                                                             CTTATATATTCCTCACGAATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATATTCATACTCTTTCTCCTTTGAGGGGCACCAAAAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTT        <  SRR19973065.2193017/150‑1 (MQ=60)
                                                                                                                                             CTGATATATTGCTCACGCATCCGTCCCAGCTTGTGTTTGACGTGTGGGTGTTTGACTTCCACTATCTTCATACTCTTTCTCATTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTT        <  SRR19973065.2772115/150‑1 (MQ=60)
                                                                                                                                             CTGATATAGTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGTGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTT        <  SRR19973065.2772159/150‑1 (MQ=60)
                                                                                                                                              TTATATATTGCTCACGAATCAGTCCCTGCTTTTGTTTGACGTGTGGGTTTTTGACTTCCACTATCTTCATACTATTTCTCCTTGGTGGGGCAGCCAAAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTC       <  SRR19973065.1274612/150‑1 (MQ=60)
                                                                                                                                                 TATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTT    >  SRR19973065.944643/1‑150 (MQ=60)
                                                                                                                                                  ATATTGCTCACGCATCAGTCCCAGCTTGTTTTTGACGAGTGGTTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAAAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTG   <  SRR19973065.2306535/150‑1 (MQ=60)
                                                                                                                                                   TATTGCTCACGCATCAGTCCAAGCTTGTTTTTGACGCGTGGTTTTTTGACTTCCACGATCTACATACTCTTTCTCATTTGAGGGGCAGCAAAAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGA  <  SRR19973065.2307788/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                    
ATTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGA  >  CP009273/2614020‑2614316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑