Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F121 I0 R1	856	273.6	9081716	94.1%	8545894	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,075,114	C→A	85.7%	intergenic (‑120/‑158)	tktA ← / → loiP	transketolase 1, thiamine triphosphate‑binding/Phe‑Phe periplasmic metalloprotease, OM lipoprotein; low salt‑inducible; heat shock protein that binds Era

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,075,114	0	C	A	85.7%	744.1 / 127.4	287	intergenic (‑120/‑158)	tktA/loiP	transketolase 1, thiamine triphosphate‑binding/Phe‑Phe periplasmic metalloprotease, OM lipoprotein; low salt‑inducible; heat shock protein that binds Era
Reads supporting (aligned to +/- strand): ref base C (22/19); new base A (133/112); total (156/131)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 288 total aligned reads displayed.

CATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTGCGGAA  >  CP009273/3074967‑3075263

 aTTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATc                                                                                                                                                    <  1:3163356/150‑1 (MQ=255)

   tGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCt                                                                                                                                                  >  2:3767164/1‑150 (MQ=255)

    ggCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCtt                                                                                                                                                 >  2:4203205/1‑150 (MQ=255)

    ggCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCtt                                                                                                                                                 <  1:4012768/150‑1 (MQ=255)

    ggCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCtt                                                                                                                                                 <  1:2822009/150‑1 (MQ=255)

      cAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCtttt                                                                                                                                               >  2:654591/1‑150 (MQ=255)

      cAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCtttt                                                                                                                                               <  2:2397641/150‑1 (MQ=255)

      cAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCtttt                                                                                                                                               >  1:1489218/1‑150 (MQ=255)

      cAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCtttt                                                                                                                                               <  2:138451/150‑1 (MQ=255)

      cAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCtgtt                                                                                                                                               >  2:2351725/1‑150 (MQ=255)

       aaGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTg                                                                                                                                              >  2:946641/1‑150 (MQ=255)

        aGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGc                                                                                                                                             >  2:1680009/1‑150 (MQ=255)

          ctctTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCg                                                                                                                                           <  1:1365318/150‑1 (MQ=255)

          ctctTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCg                                                                                                                                           <  1:1753339/150‑1 (MQ=255)

                aCGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAg                                                                                                                                     >  1:164681/1‑150 (MQ=255)

                  gTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGaa                                                                                                                                   <  2:1435343/150‑1 (MQ=255)

                  gTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGaa                                                                                                                                   <  2:1324473/150‑1 (MQ=255)

                  gTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGaa                                                                                                                                   >  1:3462472/1‑150 (MQ=255)

                       gACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAAtctc                                                                                                                              >  1:3338972/1‑150 (MQ=255)

                       gACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAAtctc                                                                                                                              >  1:1121665/1‑150 (MQ=255)

                       gACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAAtctc                                                                                                                              >  2:4068268/1‑150 (MQ=255)

                          aTTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTgg                                                                                                                           <  1:1468395/150‑1 (MQ=255)

                           ttttGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGa                                                                                                                          <  1:3650709/150‑1 (MQ=255)

                            tttGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGAt                                                                                                                         <  2:1869003/150‑1 (MQ=255)

                               gACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTGTTGCCGCTTCAGAAATCTCTGGATCAt                                                                                                                      >  1:3558154/1‑150 (MQ=255)

                                aCTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATg                                                                                                                     >  2:2798277/1‑150 (MQ=255)

                                 cTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGc                                                                                                                    >  2:2917467/1‑150 (MQ=255)

                                 cTCCAGATCGGATGATGAAGGGCACGCCCTAAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTAACCGCAAGCCGCGCGTCATGTACATGGAAAATCCATTAGCCGCTTCAGACAACTCTCGATCATGc                                                                                                                    >  1:4406132/1‑150 (MQ=255)

                                  tCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCt                                                                                                                   <  2:3286022/150‑1 (MQ=255)

                                   ccAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTc                                                                                                                  >  2:3183184/1‑150 (MQ=255)

                                   ccAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTc                                                                                                                  <  2:4490611/150‑1 (MQ=255)

                                   ccAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTc                                                                                                                  <  2:2111325/150‑1 (MQ=255)

                                     aGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGc                                                                                                                <  1:296573/150‑1 (MQ=255)

                                        tCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATg                                                                                                             <  2:3019295/150‑1 (MQ=255)

                                         cGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGt                                                                                                            >  2:2071525/1‑150 (MQ=255)

                                              gatgaAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTgcgc                                                                                                       >  1:746979/1‑150 (MQ=255)

                                                 gaAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAAt                                                                                                    >  2:823023/1‑150 (MQ=255)

                                                  aaGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATc                                                                                                   >  1:3095794/1‑150 (MQ=255)

                                                   aGGGAACGCACTTAACTACTTGACGACATCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCTCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCt                                                                                                  <  2:2719645/150‑1 (MQ=255)

                                                    gggCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTa                                                                                                 <  2:3998049/150‑1 (MQ=255)

                                                    gggCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTa                                                                                                 <  2:1267648/150‑1 (MQ=255)

                                                               tAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTcc                                                                                      <  2:1489372/150‑1 (MQ=255)

                                                                 aCGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGc                                                                                    >  1:2558698/1‑150 (MQ=255)

                                                                   gACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTg                                                                                  >  1:2245415/1‑150 (MQ=255)

                                                                        gacgacAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCtt                                                                             >  2:300421/1‑150 (MQ=255)

                                                                        gacgacAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCtt                                                                             <  2:358932/150‑1 (MQ=255)

                                                                         acgacAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCttt                                                                            >  1:4376786/1‑150 (MQ=255)

                                                                         acgacAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCttt                                                                            <  1:1262490/150‑1 (MQ=255)

                                                                           gacAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTc                                                                          <  2:314702/150‑1 (MQ=255)

                                                                           gacAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTc                                                                          >  2:2412141/1‑150 (MQ=255)

                                                                              aGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCtt                                                                       >  2:4455249/1‑150 (MQ=255)

                                                                               gcgcGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCtca                                                                      <  1:4054464/150‑3 (MQ=255)

                                                                                cgcgTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTtat                                                                     >  2:1614606/1‑150 (MQ=255)

                                                                                  cgTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAA‑ATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATact                                                                  >  2:4497841/1‑150 (MQ=255)

                                                                                   gTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATact                                                                  <  2:3570432/150‑1 (MQ=255)

                                                                                   gTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATact                                                                  >  1:1206839/1‑150 (MQ=255)

                                                                                    ttttGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATactg                                                                 <  1:2798514/150‑1 (MQ=255)

                                                                                       tGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATactgaga                                                              <  2:535942/150‑1 (MQ=255)

                                                                                       tGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATactgaga                                                              >  1:688207/1‑150 (MQ=255)

                                                                                        gggCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATactgagac                                                             <  1:2125926/150‑1 (MQ=255)

                                                                                         ggCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATactgagact                                                            <  1:827803/150‑1 (MQ=255)

                                                                                         ggCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGATGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATactgagact                                                            >  2:547410/1‑150 (MQ=255)

                                                                                          gCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATactgagactg                                                           <  2:1276883/150‑1 (MQ=255)

                                                                                           cTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATactgagactga                                                          >  1:405685/1‑150 (MQ=255)

                                                                                            tACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATactgagactgag                                                         >  2:2479915/1‑150 (MQ=255)

                                                                                             aCGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGc                                                        <  2:1555199/150‑1 (MQ=255)

                                                                                             aCGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGc                                                        <  1:1239628/150‑1 (MQ=255)

                                                                                               gCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGt                                                      >  2:4053830/1‑150 (MQ=255)

                                                                                                ccGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTc                                                     >  2:2157340/1‑150 (MQ=255)

                                                                                                 cGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCg                                                    >  1:288719/1‑150 (MQ=255)

                                                                                                  ggAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGa                                                   >  1:3528919/1‑150 (MQ=255)

                                                                                                      aaTTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCa                                               >  2:2561026/1‑150 (MQ=255)

                                                                                                      aaTTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCa                                               <  2:1733912/150‑1 (MQ=255)

                                                                                                         ttGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCt                                            <  1:963726/150‑1 (MQ=255)

                                                                                                          tGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTg                                           <  2:489405/150‑1 (MQ=255)

                                                                                                            ccAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCa                                         >  2:1076392/1‑150 (MQ=255)

                                                                                                             caacaaTTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCaa                                        <  1:4233174/150‑1 (MQ=255)

                                                                                                             caacaaTTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCaa                                        <  1:1600725/150‑1 (MQ=255)

                                                                                                              aacaaTTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCaaa                                       >  2:2936865/1‑150 (MQ=255)

                                                                                                              aacaaTTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCaaa                                       <  2:3515648/150‑1 (MQ=255)

                                                                                                               acaaTTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAAc                                      <  2:457003/150‑1 (MQ=255)

                                                                                                                 aaTTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACgg                                    >  2:2859828/1‑150 (MQ=255)

                                                                                                                  aTTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGgc                                   >  2:3827108/1‑150 (MQ=255)

                                                                                                                   tttACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGgcg                                  >  1:3404500/1‑150 (MQ=255)

                                                                                                                    ttACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGgcgc                                 >  1:1694161/1‑150 (MQ=255)

                                                                                                                     tACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCa                                <  1:578517/150‑1 (MQ=255)

                                                                                                                      aCCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCAt                               >  1:205867/1‑150 (MQ=255)

                                                                                                                        cGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCAttt                             >  1:3863251/1‑150 (MQ=255)

                                                                                                                         gCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCAtttt                            >  2:2209945/1‑150 (MQ=255)

                                                                                                                           aaGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTa                          >  2:4299124/1‑150 (MQ=255)

                                                                                                                            aGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAg                         <  1:4466637/150‑1 (MQ=255)

                                                                                                                              ccGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGaa                       >  1:471256/1‑150 (MQ=255)

                                                                                                                                 cgcgTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGaataa                    <  1:420353/150‑1 (MQ=255)

                                                                                                                                    gTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATcc                 >  1:3707548/1‑150 (MQ=255)

                                                                                                                                    gTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATcc                 <  2:2034451/150‑1 (MQ=255)

                                                                                                                                        tGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGAc             <  1:3549775/150‑1 (MQ=255)

                                                                                                                                         gTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGAcc            >  1:4079126/1‑150 (MQ=255)

                                                                                                                                          tACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCt           <  2:4159904/150‑1 (MQ=255)

                                                                                                                                              tGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTtgtg       >  2:44144/1‑150 (MQ=255)

                                                                                                                                                gAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTGCg     >  1:2667989/1‑150 (MQ=255)

CATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTGCGGAA  >  CP009273/3074967‑3075263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTGCGGAA  >  CP009273/3074967‑3075263

CATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAAT                                                                                                                                                     <  SRR19973080.3314627/150‑1 (MQ=60)

 ATTGGCAATCTCTTTACGTGAGGACATTTTTACTCCAGATAGGATGATTAAGGGCACGCCCTTAACTACTTGACGACAGCGCCTTTTGGGCTACGCCGGAAAATTTGACAACAATAAACCGCAAGCCGCGCGTCATGTAAATGGAAAATC                                                                                                                                                    >  SRR19973080.2984257/1‑150 (MQ=60)

 ATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATC                                                                                                                                                    <  SRR19973080.897699/150‑1 (MQ=60)

 ATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATC                                                                                                                                                    <  SRR19973080.3180797/150‑1 (MQ=60)

    GGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTT                                                                                                                                                 <  SRR19973080.4034786/150‑1 (MQ=60)

    GGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTT                                                                                                                                                 <  SRR19973080.2837534/150‑1 (MQ=60)

    GGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTT                                                                                                                                                 <  SRR19973080.3428872/150‑1 (MQ=60)

    GGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTT                                                                                                                                                 <  SRR19973080.3429320/150‑1 (MQ=60)

      CAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTT                                                                                                                                               >  SRR19973080.2754430/1‑150 (MQ=60)

      CAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTT                                                                                                                                               >  SRR19973080.1497758/1‑150 (MQ=60)

      CAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTT                                                                                                                                               >  SRR19973080.1497721/1‑150 (MQ=60)

       AAGCTCTTTACGTGAGGAAATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTG                                                                                                                                              <  SRR19973080.2675707/150‑1 (MQ=60)

        AGCTCTTTACGTGAGGACATTTTGACTCCAGTTCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACACTTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGC                                                                                                                                             <  SRR19973080.3601845/150‑1 (MQ=60)

        AGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGC                                                                                                                                             <  SRR19973080.4367993/150‑1 (MQ=60)

          CTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCG                                                                                                                                           <  SRR19973080.1373480/150‑1 (MQ=60)

          CTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCG                                                                                                                                           <  SRR19973080.1763451/150‑1 (MQ=60)

          CTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCG                                                                                                                                           >  SRR19973080.1634980/1‑150 (MQ=60)

          CTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCG                                                                                                                                           >  SRR19973080.1634778/1‑150 (MQ=60)

            CTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCT                                                                                                                                         >  SRR19973080.2858734/1‑150 (MQ=60)

             TTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTT                                                                                                                                        <  SRR19973080.2739332/150‑1 (MQ=60)

              TTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTC                                                                                                                                       <  SRR19973080.1503343/150‑1 (MQ=60)

               TACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCA                                                                                                                                      >  SRR19973080.371265/1‑150 (MQ=60)

                ACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAG                                                                                                                                     >  SRR19973080.165989/1‑150 (MQ=60)

                  GTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAA                                                                                                                                   >  SRR19973080.4241748/1‑150 (MQ=60)

                  GTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAA                                                                                                                                   >  SRR19973080.3481455/1‑150 (MQ=60)

                       GACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTC                                                                                                                              >  SRR19973080.1337909/1‑150 (MQ=60)

                       GACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTC                                                                                                                              >  SRR19973080.3357148/1‑150 (MQ=60)

                       GACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTC                                                                                                                              >  SRR19973080.1128102/1‑150 (MQ=60)

                          ATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGG                                                                                                                           <  SRR19973080.1476851/150‑1 (MQ=60)

                          ATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGG                                                                                                                           <  SRR19973080.1232957/150‑1 (MQ=60)

                          ATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGG                                                                                                                           >  SRR19973080.3118308/1‑150 (MQ=60)

                          ATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGG                                                                                                                           <  SRR19973080.1476910/150‑1 (MQ=60)

                           TTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGA                                                                                                                          <  SRR19973080.3670855/150‑1 (MQ=60)

                               GACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTGTTGCCGCTTCAGAAATCTCTGGATCAT                                                                                                                      >  SRR19973080.3577838/1‑150 (MQ=60)

                                 CTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGC                                                                                                                    >  SRR19973080.1770004/1‑150 (MQ=60)

                                 CTCCAGATCGGATGATGAAGGGCACGCCCTAAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTAACCGCAAGCCGCGCGTCATGTACATGGAAAATCCATTAGCCGCTTCAGACAACTCTCGATCATGC                                                                                                                    >  SRR19973080.4430108/1‑150 (MQ=60)

                                     AGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGC                                                                                                                <  SRR19973080.298461/150‑1 (MQ=60)

                                      GATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCA                                                                                                               >  SRR19973080.2139224/1‑150 (MQ=60)

                                            ATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGC                                                                                                         >  SRR19973080.1743864/1‑150 (MQ=60)

                                            ATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGC                                                                                                         >  SRR19973080.3363415/1‑150 (MQ=60)

                                            ATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGC                                                                                                         >  SRR19973080.1743941/1‑150 (MQ=60)

                                             TGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCG                                                                                                        >  SRR19973080.3905067/1‑150 (MQ=60)

                                              GATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGC                                                                                                       >  SRR19973080.751215/1‑150 (MQ=60)

                                                  AAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATC                                                                                                   >  SRR19973080.3112756/1‑150 (MQ=60)

                                                  AAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATC                                                                                                   >  SRR19973080.3112740/1‑150 (MQ=60)

                                                      GCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAACTCTCTGGATCATGCTCGCATGTTGCGCAATCTACT                                                                                               >  SRR19973080.4055584/1‑150 (MQ=60)

                                                      GCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACT                                                                                               >  SRR19973080.4055463/1‑150 (MQ=60)

                                                         CGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGC                                                                                            >  SRR19973080.1393593/1‑150 (MQ=60)

                                                         CGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGC                                                                                            >  SRR19973080.815187/1‑150 (MQ=60)

                                                          GCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCC                                                                                           >  SRR19973080.3607735/1‑150 (MQ=60)

                                                             CTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGT                                                                                        <  SRR19973080.769476/150‑1 (MQ=60)

                                                              TTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTC                                                                                       >  SRR19973080.352939/1‑150 (MQ=60)

                                                              TTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTC                                                                                       >  SRR19973080.4403741/1‑150 (MQ=60)

                                                               TAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCC                                                                                      <  SRR19973080.2094683/150‑1 (MQ=60)

                                                                 ACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGC                                                                                    >  SRR19973080.2572695/1‑150 (MQ=60)

                                                                   GACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTG                                                                                  <  SRR19973080.2841532/150‑1 (MQ=60)

                                                                   GACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTG                                                                                  >  SRR19973080.4544832/1‑150 (MQ=60)

                                                                   GACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTG                                                                                  >  SRR19973080.2257726/1‑150 (MQ=60)

                                                                     CTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCG                                                                                <  SRR19973080.2074407/150‑1 (MQ=60)

                                                                         ACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTT                                                                            <  SRR19973080.1270137/150‑1 (MQ=60)

                                                                         ACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTT                                                                            <  SRR19973080.543752/150‑1 (MQ=60)

                                                                         ACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTT                                                                            >  SRR19973080.4400995/1‑150 (MQ=60)

                                                                         ACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTT                                                                            >  SRR19973080.4400639/1‑150 (MQ=60)

                                                                           GACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTC                                                                          <  SRR19973080.44835/150‑1 (MQ=60)

                                                                               GCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTA                                                                      <  SRR19973080.4076882/150‑1 (MQ=60)

                                                                               GCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTA                                                                      <  SRR19973080.4076860/150‑1 (MQ=60)

                                                                               GCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTCA                                                                      <  SRR19973080.4076628/150‑1 (MQ=60)

                                                                                  CGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATAC                                                                   >  SRR19973080.3570742/1‑150 (MQ=60)

                                                                                  CGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATAC                                                                   >  SRR19973080.3570513/1‑150 (MQ=60)

                                                                                   GTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACT                                                                  >  SRR19973080.1214088/1‑150 (MQ=60)

                                                                                   GTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACT                                                                  >  SRR19973080.1214130/1‑150 (MQ=60)

                                                                                    TTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTG                                                                 <  SRR19973080.2813940/150‑1 (MQ=60)

                                                                                    TTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTG                                                                 <  SRR19973080.2815488/150‑1 (MQ=60)

                                                                                       TGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGA                                                              >  SRR19973080.692301/1‑150 (MQ=60)

                                                                                       TGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGA                                                              >  SRR19973080.694065/1‑150 (MQ=60)

                                                                                       TGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGA                                                              >  SRR19973080.692272/1‑150 (MQ=60)

                                                                                        GGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGAC                                                             <  SRR19973080.2137901/150‑1 (MQ=60)

                                                                                         GGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACT                                                            <  SRR19973080.2933356/150‑1 (MQ=60)

                                                                                         GGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACT                                                            <  SRR19973080.832741/150‑1 (MQ=60)

                                                                                           CTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGA                                                          >  SRR19973080.408125/1‑150 (MQ=60)

                                                                                           CTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGA                                                          >  SRR19973080.408161/1‑150 (MQ=60)

                                                                                            TACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAG                                                         >  SRR19973080.3874664/1‑150 (MQ=60)

                                                                                            TACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAG                                                         >  SRR19973080.337284/1‑150 (MQ=60)

                                                                                             ACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGC                                                        <  SRR19973080.1247129/150‑1 (MQ=60)

                                                                                                 CGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCG                                                    >  SRR19973080.290583/1‑150 (MQ=60)

                                                                                                  GGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGA                                                   >  SRR19973080.3548436/1‑150 (MQ=60)

                                                                                                    AAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATT                                                 >  SRR19973080.2148316/1‑150 (MQ=60)

                                                                                                    AAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATT                                                 >  SRR19973080.3672976/1‑150 (MQ=60)

                                                                                                         TTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCT                                            <  SRR19973080.2408429/150‑1 (MQ=60)

                                                                                                         TTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCT                                            <  SRR19973080.969566/150‑1 (MQ=60)

                                                                                                         TTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCT                                            <  SRR19973080.2408410/150‑1 (MQ=60)

                                                                                                         TTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCT                                            <  SRR19973080.4199604/150‑1 (MQ=60)

                                                                                                          TGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTG                                           >  SRR19973080.3884805/1‑150 (MQ=60)

                                                                                                            CCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCA                                         <  SRR19973080.881082/150‑1 (MQ=60)

                                                                                                             CAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAA                                        <  SRR19973080.1610088/150‑1 (MQ=60)

                                                                                                             CAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAA                                        <  SRR19973080.4258522/150‑1 (MQ=60)

                                                                                                             CAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAA                                        <  SRR19973080.4256234/150‑1 (MQ=60)

                                                                                                                CAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACG                                     <  SRR19973080.3565693/150‑1 (MQ=60)

                                                                                                                  ATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGC                                   >  SRR19973080.1225196/1‑150 (MQ=60)

                                                                                                                   TTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCG                                  >  SRR19973080.3422911/1‑150 (MQ=60)

                                                                                                                    TTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGC                                 >  SRR19973080.1704025/1‑150 (MQ=60)

                                                                                                                     TACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCA                                <  SRR19973080.582137/150‑1 (MQ=60)

                                                                                                                      ACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCAT                               >  SRR19973080.207396/1‑150 (MQ=60)

                                                                                                                        CGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTT                             >  SRR19973080.3884520/1‑150 (MQ=60)

                                                                                                                            AGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAG                         <  SRR19973080.4226042/150‑1 (MQ=60)

                                                                                                                            AGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAG                         <  SRR19973080.4490798/150‑1 (MQ=60)

                                                                                                                            AGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAG                         <  SRR19973080.4490832/150‑1 (MQ=60)

                                                                                                                              CCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAA                       >  SRR19973080.474246/1‑150 (MQ=60)

                                                                                                                                GCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATA                     <  SRR19973080.2580542/150‑1 (MQ=60)

                                                                                                                                GCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATA                     >  SRR19973080.351611/1‑150 (MQ=60)

                                                                                                                                 CGCTTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAA                    <  SRR19973080.423232/150‑1 (MQ=60)

                                                                                                                                 CGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAA                    <  SRR19973080.422921/150‑1 (MQ=60)

                                                                                                                                   CGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATGTTTAGAATAATC                  >  SRR19973080.551190/1‑150 (MQ=60)

                                                                                                                                    GTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCC                 >  SRR19973080.3727893/1‑150 (MQ=60)

                                                                                                                                    GTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCC                 >  SRR19973080.322952/1‑150 (MQ=60)

                                                                                                                                    GTCATGTACATGCAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCC                 <  SRR19973080.3815482/150‑1 (MQ=60)

                                                                                                                                      CATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTG               <  SRR19973080.1269749/150‑1 (MQ=60)

                                                                                                                                      CATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTG               <  SRR19973080.1269804/150‑1 (MQ=60)

                                                                                                                                        TGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGAC             <  SRR19973080.3569403/150‑1 (MQ=60)

                                                                                                                                        TGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGAC             <  SRR19973080.1274994/150‑1 (MQ=60)

                                                                                                                                        TGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGAC             <  SRR19973080.1489974/150‑1 (MQ=60)

                                                                                                                                        TGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGAC             <  SRR19973080.471516/150‑1 (MQ=60)

                                                                                                                                         GTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACC            >  SRR19973080.4101369/1‑150 (MQ=60)

                                                                                                                                         GTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACC            >  SRR19973080.3128568/1‑150 (MQ=60)

                                                                                                                                             ATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGT        <  SRR19973080.1461803/150‑1 (MQ=60)

                                                                                                                                               GGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGATTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTGC      <  SRR19973080.658533/150‑1 (MQ=60)

                                                                                                                                                GAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTGCG     >  SRR19973080.2682489/1‑150 (MQ=60)

                                                                                                                                                GAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTGCG     >  SRR19973080.2906936/1‑150 (MQ=60)

                                                                                                                                                GAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTGCG     <  SRR19973080.1689813/150‑1 (MQ=60)

                                                                                                                                                GAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTGCG     <  SRR19973080.1887020/150‑1 (MQ=60)

                                                                                                                                                   AATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTGCGGAA  <  SRR19973080.4091139/150‑1 (MQ=60)

                                                                                                                                                   AATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTGCGGAA  <  SRR19973080.4090475/150‑1 (MQ=60)

                                                                                                                                                   AATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTGCGGAA  <  SRR19973080.2160114/150‑1 (MQ=60)

CATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTGCGGAA  >  CP009273/3074967‑3075263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`