Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F98 I0 R1 799 294.1 9752082 92.2% 8991419 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,075,114 C→A 67.2% intergenic (‑120/‑158) tktA ← / → loiP transketolase 1, thiamine triphosphate‑binding/Phe‑Phe periplasmic metalloprotease, OM lipoprotein; low salt‑inducible; heat shock protein that binds Era

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,075,1140CA67.2% 301.3 / 304.3 262intergenic (‑120/‑158)tktA/loiPtransketolase 1, thiamine triphosphate‑binding/Phe‑Phe periplasmic metalloprotease, OM lipoprotein; low salt‑inducible; heat shock protein that binds Era
Reads supporting (aligned to +/- strand):  ref base C (38/48);  new base A (82/94);  total (120/142)
Fisher's exact test for biased strand distribution p-value = 7.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 269 total aligned reads displayed.

AGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTGCGGAA  >  CP009273/3074965‑3075263
                                                                                                                                                     |                                                                                                                                                     
aGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAc                                                                                                                                                       <  2:2548157/150‑1 (MQ=255)
  ctttGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAAt                                                                                                                                                     <  1:2869787/148‑1 (MQ=255)
  cATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAAt                                                                                                                                                     <  1:437937/150‑1 (MQ=255)
    ttGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATcc                                                                                                                                                   <  2:3474460/150‑1 (MQ=255)
    ttGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATcc                                                                                                                                                   <  2:4561964/150‑1 (MQ=255)
     tGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCt                                                                                                                                                  <  2:3672520/150‑1 (MQ=255)
     tGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCt                                                                                                                                                  <  2:100903/150‑1 (MQ=255)
     tGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAGTCCt                                                                                                                                                  >  2:628907/1‑150 (MQ=255)
      ggCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCtt                                                                                                                                                 >  2:2226378/1‑150 (MQ=255)
      ggCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCtt                                                                                                                                                 <  2:3651529/150‑1 (MQ=255)
        cAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCtttt                                                                                                                                               >  1:2649983/1‑150 (MQ=255)
          aGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGc                                                                                                                                             <  1:3887750/150‑1 (MQ=255)
             tctTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGc                                                                                                                                          >  2:73834/1‑150 (MQ=255)
              ctTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCt                                                                                                                                         <  1:1845526/150‑1 (MQ=255)
              ctTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCt                                                                                                                                         >  1:1245029/1‑150 (MQ=255)
               tttACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCtt                                                                                                                                        <  2:4253841/150‑1 (MQ=255)
               tttACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCtt                                                                                                                                        <  2:1697230/150‑1 (MQ=255)
               tttACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCtt                                                                                                                                        >  2:4570587/1‑150 (MQ=255)
                ttACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTc                                                                                                                                       >  1:1844754/1‑150 (MQ=255)
                 tACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCa                                                                                                                                      >  1:2896656/1‑150 (MQ=255)
                  aCGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCATTAACTACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAg                                                                                                                                     <  1:4573912/150‑1 (MQ=255)
                    gTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGaa                                                                                                                                   >  2:3187486/1‑150 (MQ=255)
                      gAGGACATTGTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAAt                                                                                                                                 <  1:2989556/150‑1 (MQ=255)
                       aGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAAtc                                                                                                                                >  1:4548328/1‑150 (MQ=255)
                          aCATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATctct                                                                                                                             <  1:4792068/150‑1 (MQ=255)
                          aCATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATctct                                                                                                                             <  1:1981272/150‑1 (MQ=255)
                           cATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTg                                                                                                                            >  2:4313984/1‑150 (MQ=255)
                            aTTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTgg                                                                                                                           <  1:2159491/150‑1 (MQ=255)
                             ttttGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGa                                                                                                                          <  2:1334950/150‑1 (MQ=255)
                                    tCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCt                                                                                                                   >  2:3394552/1‑150 (MQ=255)
                                    tCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCt                                                                                                                   <  1:4162449/150‑1 (MQ=255)
                                     ccAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTc                                                                                                                  <  1:4342703/150‑1 (MQ=255)
                                      cAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCg                                                                                                                 >  2:4389721/1‑150 (MQ=255)
                                      cAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCg                                                                                                                 <  2:1679109/150‑1 (MQ=255)
                                         aTCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCAt                                                                                                              <  2:3435128/150‑1 (MQ=255)
                                            ggATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGtt                                                                                                           >  2:4175604/1‑150 (MQ=255)
                                            ggATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTCGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGtt                                                                                                           <  1:1270053/150‑1 (MQ=255)
                                              atgatgAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTgc                                                                                                         >  1:2040802/1‑150 (MQ=255)
                                                   gaAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAAt                                                                                                    >  2:2553053/1‑150 (MQ=255)
                                                   gaAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAAt                                                                                                    <  2:2519332/150‑1 (MQ=255)
                                                    aaGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATc                                                                                                   <  2:2149326/150‑1 (MQ=255)
                                                     aGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCt                                                                                                  >  2:1540794/1‑150 (MQ=255)
                                                      gggCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTa                                                                                                 <  2:2650035/150‑1 (MQ=255)
                                                         cACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTc                                                                                              <  2:1844809/150‑1 (MQ=255)
                                                           cGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGc                                                                                            >  2:2677165/1‑150 (MQ=255)
                                                              ccTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCg                                                                                         <  2:186042/150‑1 (MQ=255)
                                                               cTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGt                                                                                        <  2:411098/150‑1 (MQ=255)
                                                               cTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGt                                                                                        >  1:502719/1‑150 (MQ=255)
                                                                ttAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTc                                                                                       >  2:2862408/1‑150 (MQ=255)
                                                                  aaCGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCg                                                                                     <  2:1502803/150‑1 (MQ=255)
                                                                     gACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTg                                                                                  >  1:3180570/1‑150 (MQ=255)
                                                                       cTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTgcg                                                                                >  1:722446/1‑150 (MQ=255)
                                                                           acgacAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCttt                                                                            <  2:1917770/150‑1 (MQ=255)
                                                                             gacAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTc                                                                          <  2:2093933/150‑1 (MQ=255)
                                                                             gacAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTc                                                                          <  1:741057/150‑1 (MQ=255)
                                                                                aGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCtt                                                                       <  1:3965172/150‑1 (MQ=255)
                                                                                  cgcgTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTtat                                                                     >  2:4792706/1‑150 (MQ=255)
                                                                                  cgcgTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTtat                                                                     >  2:4792701/1‑150 (MQ=255)
                                                                                     gTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATact                                                                  >  2:3809709/1‑150 (MQ=255)
                                                                                     gTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATact                                                                  <  1:1037712/150‑1 (MQ=255)
                                                                                     gTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATact                                                                  >  2:4752127/1‑150 (MQ=255)
                                                                                       tttGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATactga                                                                <  1:3447893/150‑1 (MQ=255)
                                                                                         tGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATactgaga                                                              <  2:700627/150‑1 (MQ=255)
                                                                                          gggCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATactgagac                                                             <  2:2703406/150‑1 (MQ=255)
                                                                                           ggCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCGTTTCCTTATactgagact                                                            <  1:2351080/150‑1 (MQ=255)
                                                                                             cTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATactgagactga                                                          >  2:3274446/1‑150 (MQ=255)
                                                                                                cGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCg                                                       <  2:3826762/150‑1 (MQ=255)
                                                                                                  ccGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTc                                                     >  2:2659040/1‑150 (MQ=255)
                                                                                                   cGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCg                                                    >  2:525690/1‑150 (MQ=255)
                                                                                                      aaaaTTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGAtt                                                 <  2:4054131/150‑1 (MQ=255)
                                                                                                        aaTTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGTAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCa                                               <  1:4078858/150‑1 (MQ=255)
                                                                                                        aaTTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCa                                               <  1:1255338/150‑1 (MQ=255)
                                                                                                           ttGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCt                                            <  2:4548328/150‑1 (MQ=255)
                                                                                                            tGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTg                                           <  1:1493254/150‑1 (MQ=255)
                                                                                                            tGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTg                                           <  2:4728905/150‑1 (MQ=255)
                                                                                                             gCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGc                                          >  2:119774/1‑150 (MQ=255)
                                                                                                             gCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGc                                          >  2:1826277/1‑150 (MQ=255)
                                                                                                                  caaTTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACg                                     >  2:3904461/1‑150 (MQ=255)
                                                                                                                    aTTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGgc                                   <  1:356534/150‑1 (MQ=255)
                                                                                                                    aTTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGgc                                   >  2:2342892/1‑150 (MQ=255)
                                                                                                                    aTTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGgc                                   >  2:4421727/1‑150 (MQ=255)
                                                                                                                       tACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCa                                <  2:2956452/150‑1 (MQ=255)
                                                                                                                         ccGCAAGCCGCGCGTCATGTAAATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGAAATCTACTCGCCCGTCCGCTGCGCTTTTCATTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCAtt                              <  1:1600046/150‑1 (MQ=255)
                                                                                                                           gCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCAtttt                            <  1:2442198/150‑1 (MQ=255)
                                                                                                                            cAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCAttttt                           <  1:845514/150‑1 (MQ=255)
                                                                                                                            cAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCAttttt                           <  1:1102366/150‑1 (MQ=255)
                                                                                                                             aaGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTa                          >  2:1153799/1‑150 (MQ=255)
                                                                                                                              aGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAg                         <  1:3546164/150‑1 (MQ=255)
                                                                                                                                 cgcgcgTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGaat                      >  2:1736090/1‑150 (MQ=255)
                                                                                                                                   cgcgTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGaataa                    >  2:4760706/1‑150 (MQ=255)
                                                                                                                                      gTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATcc                 >  1:2735824/1‑150 (MQ=255)
                                                                                                                                      gTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATcc                 <  1:4570577/150‑1 (MQ=255)
                                                                                                                                          tGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGAc             >  2:3560721/1‑150 (MQ=255)
                                                                                                                                             aCATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCtt          <  2:79022/150‑1 (MQ=255)
                                                                                                                                               aTGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTtgt        >  1:680116/1‑150 (MQ=255)
                                                                                                                                               aTGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTtgt        <  1:4527132/150‑1 (MQ=255)
                                                                                                                                                tGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTtgtg       >  2:4701193/1‑150 (MQ=255)
                                                                                                                                                  gAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTGCg     <  1:603512/150‑1 (MQ=255)
                                                                                                                                                   aaCATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTGCgg    <  2:3274267/150‑1 (MQ=255)
                                                                                                                                                     cATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTGCGGaa  <  1:3438705/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTGCGGAA  >  CP009273/3074965‑3075263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTGCGGAA  >  CP009273/3074965‑3075263
                                                                                                                                                     |                                                                                                                                                     
AGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAC                                                                                                                                                       <  SRR19973081.4704180/150‑1 (MQ=60)
  CTTTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAAT                                                                                                                                                     <  SRR19973081.2887330/150‑1 (MQ=60)
  CATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAAT                                                                                                                                                     <  SRR19973081.440896/150‑1 (MQ=60)
     TGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCT                                                                                                                                                  >  SRR19973081.2720138/1‑150 (MQ=60)
      GGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTT                                                                                                                                                 >  SRR19973081.928066/1‑150 (MQ=60)
        CAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTT                                                                                                                                               >  SRR19973081.2666467/1‑150 (MQ=60)
        CAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTT                                                                                                                                               >  SRR19973081.2666519/1‑150 (MQ=60)
         AAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTG                                                                                                                                              <  SRR19973081.4567515/150‑1 (MQ=60)
          AGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGC                                                                                                                                             <  SRR19973081.3911640/150‑1 (MQ=60)
              CTTTAGGTGTGGACATTTTGACTCCAGATCGGATGTTGATGGGCACGCCCTTAACGACTTGACGTCAGCGCGTTTTGGGCTTCGCCGGAAAATTTGCCAACATTTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCT                                                                                                                                         <  SRR19973081.1857394/150‑1 (MQ=60)
              CTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCT                                                                                                                                         >  SRR19973081.1253227/1‑150 (MQ=60)
              CTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCT                                                                                                                                         <  SRR19973081.1857492/150‑1 (MQ=60)
               tttactttagtaatTTTTGACTCAATATCGGATGATGAAGGTCACGCCCTTAACGAATTGACGACAGCGAGTTTTGGGCTACTCCGGAAAATTTTCCAACAATTTACCGCAAGCCGCGCGTAATGTACATGGAACATCCTTTTGCCGCTT                                                                                                                                        <  SRR19973081.2942770/136‑1 (MQ=60)
                TTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTC                                                                                                                                       >  SRR19973081.1856772/1‑150 (MQ=60)
                TTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTC                                                                                                                                       >  SRR19973081.1856716/1‑150 (MQ=60)
                TTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTC                                                                                                                                       >  SRR19973081.292048/1‑150 (MQ=60)
                 TACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCA                                                                                                                                      >  SRR19973081.2914470/1‑150 (MQ=60)
                  ACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCATTAACTACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAG                                                                                                                                     <  SRR19973081.4601657/150‑1 (MQ=60)
                   CGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGA                                                                                                                                    >  SRR19973081.3878725/1‑150 (MQ=60)
                   CGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGA                                                                                                                                    >  SRR19973081.1092742/1‑150 (MQ=60)
                   CGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGA                                                                                                                                    >  SRR19973081.1212929/1‑150 (MQ=60)
                     TGAGTAAATTTTGACTCCATATCGGATGATGATGGGCACGCCATTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAA                                                                                                                                  <  SRR19973081.3723801/150‑1 (MQ=60)
                      GAGGACATTGTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAAT                                                                                                                                 <  SRR19973081.3007983/150‑1 (MQ=60)
                       AGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATC                                                                                                                                >  SRR19973081.4575908/1‑150 (MQ=60)
                       AGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATC                                                                                                                                >  SRR19973081.4575898/1‑150 (MQ=60)
                       AGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATC                                                                                                                                >  SRR19973081.4575913/1‑150 (MQ=60)
                        GGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCT                                                                                                                               >  SRR19973081.3885743/1‑150 (MQ=60)
                        GGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCT                                                                                                                               <  SRR19973081.3949728/150‑1 (MQ=60)
                        GGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCT                                                                                                                               <  SRR19973081.3950187/150‑1 (MQ=60)
                          ACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCT                                                                                                                             <  SRR19973081.4820962/150‑1 (MQ=60)
                          ACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCT                                                                                                                             <  SRR19973081.1993698/150‑1 (MQ=60)
                          ACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCT                                                                                                                             <  SRR19973081.1993696/150‑1 (MQ=60)
                            ATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGG                                                                                                                           <  SRR19973081.2171815/150‑1 (MQ=60)
                            ATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGG                                                                                                                           <  SRR19973081.2173251/150‑1 (MQ=60)
                            ATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGG                                                                                                                           >  SRR19973081.2184994/1‑150 (MQ=60)
                              TTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGAT                                                                                                                         <  SRR19973081.445936/150‑1 (MQ=60)
                                 GACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCAT                                                                                                                      >  SRR19973081.721860/1‑150 (MQ=60)
                                 GACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCAT                                                                                                                      >  SRR19973081.721862/1‑150 (MQ=60)
                                    TCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCT                                                                                                                   <  SRR19973081.4187884/150‑1 (MQ=60)
                                    TCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCT                                                                                                                   <  SRR19973081.1603516/150‑1 (MQ=60)
                                     CCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTC                                                                                                                  <  SRR19973081.4369048/150‑1 (MQ=60)
                                     CCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTC                                                                                                                  >  SRR19973081.1675401/1‑150 (MQ=60)
                                       AGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGC                                                                                                                >  SRR19973081.2150109/1‑150 (MQ=60)
                                       AGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGC                                                                                                                >  SRR19973081.1253539/1‑150 (MQ=60)
                                       AGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGC                                                                                                                >  SRR19973081.1253455/1‑150 (MQ=60)
                                            GGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTCGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTT                                                                                                           <  SRR19973081.1278457/150‑1 (MQ=60)
                                             GATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTG                                                                                                          <  SRR19973081.1353270/150‑1 (MQ=60)
                                              ATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGC                                                                                                         >  SRR19973081.2053624/1‑150 (MQ=60)
                                                 ATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCA                                                                                                      <  SRR19973081.1370752/150‑1 (MQ=60)
                                                 ATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCA                                                                                                      >  SRR19973081.4561464/1‑150 (MQ=60)
                                                 ATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCA                                                                                                      >  SRR19973081.3119475/1‑150 (MQ=60)
                                                         CACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTC                                                                                              <  SRR19973081.4899999/150‑1 (MQ=60)
                                                               CTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGT                                                                                        >  SRR19973081.506309/1‑150 (MQ=60)
                                                                 TAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCC                                                                                      >  SRR19973081.2904318/1‑150 (MQ=60)
                                                                 TAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCC                                                                                      >  SRR19973081.4872950/1‑150 (MQ=60)
                                                                    CGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCT                                                                                   <  SRR19973081.91556/150‑1 (MQ=60)
                                                                     GACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTG                                                                                  >  SRR19973081.3199893/1‑150 (MQ=60)
                                                                     GACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTG                                                                                  >  SRR19973081.2520506/1‑150 (MQ=60)
                                                                     GACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTG                                                                                  >  SRR19973081.772376/1‑150 (MQ=60)
                                                                       CTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCG                                                                                >  SRR19973081.727224/1‑150 (MQ=60)
                                                                             GACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTC                                                                          <  SRR19973081.4340223/150‑1 (MQ=60)
                                                                             GACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTC                                                                          <  SRR19973081.745901/150‑1 (MQ=60)
                                                                                AGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTT                                                                       <  SRR19973081.3989398/150‑1 (MQ=60)
                                                                                     GTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACT                                                                  <  SRR19973081.1044691/150‑1 (MQ=60)
                                                                                      TTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTG                                                                 <  SRR19973081.4361023/150‑1 (MQ=60)
                                                                                       TTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGA                                                                <  SRR19973081.3469109/150‑1 (MQ=60)
                                                                                         TGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGA                                                              <  SRR19973081.2556253/150‑1 (MQ=60)
                                                                                           GGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCGTTTCCTTATACTGAGACT                                                            <  SRR19973081.2365719/150‑1 (MQ=60)
                                                                                               ACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGC                                                        <  SRR19973081.2293487/150‑1 (MQ=60)
                                                                                                 GCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGT                                                      >  SRR19973081.1607304/1‑150 (MQ=60)
                                                                                                 GCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGT                                                      >  SRR19973081.346960/1‑150 (MQ=60)
                                                                                                   CGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCG                                                    <  SRR19973081.3117702/150‑1 (MQ=60)
                                                                                                     GAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCATAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGAT                                                  <  SRR19973081.4689271/150‑1 (MQ=60)
                                                                                                     GAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGAT                                                  <  SRR19973081.4000409/150‑1 (MQ=60)
                                                                                                     GAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGAT                                                  >  SRR19973081.1113275/1‑150 (MQ=60)
                                                                                                      AAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATT                                                 >  SRR19973081.79531/1‑150 (MQ=60)
                                                                                                        AATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGTAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCA                                               <  SRR19973081.4103580/150‑1 (MQ=60)
                                                                                                        AATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCA                                               <  SRR19973081.1263621/150‑1 (MQ=60)
                                                                                                           TTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCT                                            <  SRR19973081.1275912/150‑1 (MQ=60)
                                                                                                           TTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGATTTTCCTTATACTGAGACTGAGCGTCGATTCACCT                                            <  SRR19973081.519923/150‑1 (MQ=60)
                                                                                                            TGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTG                                           <  SRR19973081.1503124/150‑1 (MQ=60)
                                                                                                              CCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCA                                         >  SRR19973081.204402/1‑150 (MQ=60)
                                                                                                              CCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCA                                         >  SRR19973081.350374/1‑150 (MQ=60)
                                                                                                                    ATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGC                                   <  SRR19973081.358984/150‑1 (MQ=60)
                                                                                                                    ATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGC                                   <  SRR19973081.357783/150‑1 (MQ=60)
                                                                                                                         CCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATT                              <  SRR19973081.610406/150‑1 (MQ=60)
                                                                                                                         CCGCAAGCCGCGCGTCATGTAAATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGAAATCTACTCGCCCGTCCGCTGCGCTTTTCATTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATT                              <  SRR19973081.1610296/150‑1 (MQ=60)
                                                                                                                          CGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTT                             <  SRR19973081.2846366/150‑1 (MQ=60)
                                                                                                                          CGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTT                             >  SRR19973081.2424362/1‑150 (MQ=60)
                                                                                                                          CGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTT                             <  SRR19973081.611324/150‑1 (MQ=60)
                                                                                                                           GCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTT                            <  SRR19973081.1899702/150‑1 (MQ=60)
                                                                                                                           GCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTT                            <  SRR19973081.2457234/150‑1 (MQ=60)
                                                                                                                            CAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTT                           <  SRR19973081.850972/150‑1 (MQ=60)
                                                                                                                            CAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTT                           <  SRR19973081.1109637/150‑1 (MQ=60)
                                                                                                                             AAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTA                          <  SRR19973081.2934897/150‑1 (MQ=60)
                                                                                                                              AGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAG                         <  SRR19973081.3567822/150‑1 (MQ=60)
                                                                                                                              AGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAG                         <  SRR19973081.3567632/150‑1 (MQ=60)
                                                                                                                                 CGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAAT                      >  SRR19973081.4384832/1‑150 (MQ=60)
                                                                                                                                 CGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTGTTAGAAT                      >  SRR19973081.4384794/1‑150 (MQ=60)
                                                                                                                                     CGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATC                  <  SRR19973081.74661/150‑1 (MQ=60)
                                                                                                                                      GTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCC                 >  SRR19973081.2752686/1‑150 (MQ=60)
                                                                                                                                      GTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCC                 >  SRR19973081.2752691/1‑150 (MQ=60)
                                                                                                                                      GTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCC                 <  SRR19973081.4598301/150‑1 (MQ=60)
                                                                                                                                      GTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCC                 <  SRR19973081.4598291/150‑1 (MQ=60)
                                                                                                                                        CTTGTACTTGGAACATCGTTTTGCCGCTTCAGATATCTGTGGTTCTTGGTGGCTTGTTGCGCAATGTACTCGCCCGTCGGGTGCGGTTTTCCTTTTACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTG               <  SRR19973081.417009/150‑1 (MQ=60)
                                                                                                                                        AATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTG               <  SRR19973081.416571/150‑1 (MQ=60)
                                                                                                                                               ATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGT        >  SRR19973081.684706/1‑150 (MQ=60)
                                                                                                                                               ATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGT        <  SRR19973081.4554537/150‑1 (MQ=60)
                                                                                                                                                  GAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTGCG     <  SRR19973081.607755/150‑1 (MQ=60)
                                                                                                                                                  GAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTGCG     <  SRR19973081.3452408/150‑1 (MQ=60)
                                                                                                                                                   AAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTGCGG    >  SRR19973081.889969/1‑150 (MQ=60)
                                                                                                                                                     CATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTGCGGAA  <  SRR19973081.3459855/150‑1 (MQ=60)
                                                                                                                                                     CATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTGCGGAA  <  SRR19973081.3462354/150‑1 (MQ=60)
                                                                                                                                                     AATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTGCGGAA  >  SRR19973081.225652/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
AGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTGCGGAA  >  CP009273/3074965‑3075263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑