Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F218 I0 R1	1506	278.7	9275112	94.5%	8764980	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,075,114	C→A	100%	intergenic (‑120/‑158)	tktA ← / → loiP	transketolase 1, thiamine triphosphate‑binding/Phe‑Phe periplasmic metalloprotease, OM lipoprotein; low salt‑inducible; heat shock protein that binds Era

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,075,114	0	C	A	96.8%	916.8 / 16.3	278	intergenic (‑120/‑158)	tktA/loiP	transketolase 1, thiamine triphosphate‑binding/Phe‑Phe periplasmic metalloprotease, OM lipoprotein; low salt‑inducible; heat shock protein that binds Era
Reads supporting (aligned to +/- strand): ref base C (5/5); new base A (132/136); total (137/141)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 278 total aligned reads displayed.

GCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTGCG  >  CP009273/3074966‑3075260

 cATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAAt                                                                                                                                                  <  1:1646929/150‑1 (MQ=255)

  aTTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATc                                                                                                                                                 >  2:4041721/1‑150 (MQ=255)

   ttGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATcc                                                                                                                                                <  2:1965161/150‑1 (MQ=255)

    tGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCt                                                                                                                                               >  2:739749/1‑150 (MQ=255)

       cAAGCTCTTTACGTGAGGACATTTGGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCtttt                                                                                                                                            <  2:2176584/150‑1 (MQ=255)

         aGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGc                                                                                                                                          >  1:2633173/1‑150 (MQ=255)

             ctTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCt                                                                                                                                      >  1:3395456/1‑150 (MQ=255)

              tttACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCtt                                                                                                                                     <  2:4174326/150‑1 (MQ=255)

              tttACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCtt                                                                                                                                     <  1:3128693/150‑1 (MQ=255)

                tACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCa                                                                                                                                   <  2:870541/150‑1 (MQ=255)

                   gTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGaa                                                                                                                                >  1:471247/1‑150 (MQ=255)

                    tGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGaaa                                                                                                                               >  2:1922724/1‑150 (MQ=255)

                     gAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAAt                                                                                                                              <  1:433268/150‑1 (MQ=255)

                         aCATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATctct                                                                                                                          <  1:1181520/150‑1 (MQ=255)

                           aTTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCATTTTGCCGCTTCAGAAATCTCTgt                                                                                                                        >  2:3918286/1‑149 (MQ=255)

                             tttGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGAt                                                                                                                      <  2:1929910/150‑1 (MQ=255)

                              ttGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATc                                                                                                                     <  1:1302197/150‑1 (MQ=255)

                               tGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCa                                                                                                                    >  2:2048165/1‑150 (MQ=255)

                                gACTCCAGATCGGTTGATGAAGGGCTCGCCCTTAACGACTTGACGACAGCGCGTATTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCTCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCAt                                                                                                                   <  2:225547/150‑1 (MQ=255)

                                gACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCAt                                                                                                                   <  2:2744302/150‑1 (MQ=255)

                                gACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCAt                                                                                                                   <  1:2457809/150‑1 (MQ=255)

                                gACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTAAGAAATCTCTGGATCAt                                                                                                                   >  2:269/1‑150 (MQ=255)

                                 aCTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATg                                                                                                                  >  1:988091/1‑150 (MQ=255)

                                 aCTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATg                                                                                                                  >  1:310300/1‑150 (MQ=255)

                                 aCTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATg                                                                                                                  <  2:3580735/150‑1 (MQ=255)

                                     cAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCg                                                                                                              >  1:1859846/1‑150 (MQ=255)

                                      aGATCGGATGATGAAGGGCACGCCCTTAACTACTTGACGACAGCGCGATTTGGTCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCga                                                                                                             >  1:1977375/1‑149 (MQ=255)

                                       gATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCa                                                                                                            >  1:409010/1‑150 (MQ=255)

                                        aTCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCAt                                                                                                           >  2:3596281/1‑150 (MQ=255)

                                         tCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATg                                                                                                          >  2:3670143/1‑150 (MQ=255)

                                         tCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATg                                                                                                          >  2:2982151/1‑150 (MQ=255)

                                           ggATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGtt                                                                                                        <  2:3334718/150‑1 (MQ=255)

                                              tgatgaAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTgcg                                                                                                     >  2:1079855/1‑150 (MQ=255)

                                              tgatgaAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTgcg                                                                                                     <  1:4269227/150‑1 (MQ=255)

                                                atgaAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCa                                                                                                   >  1:1754237/1‑150 (MQ=255)

                                                 tgaAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCaa                                                                                                  >  1:3772521/1‑150 (MQ=255)

                                                      ggCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTAc                                                                                             <  2:1380095/150‑1 (MQ=255)

                                                       gCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACt                                                                                            >  2:2565285/1‑150 (MQ=255)

                                                         aCGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCg                                                                                          <  1:1260781/150‑1 (MQ=255)

                                                            cccTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGccc                                                                                       >  2:2473070/1‑150 (MQ=255)

                                                             ccTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCg                                                                                      <  2:1765891/150‑1 (MQ=255)

                                                                tAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTcc                                                                                   >  2:668234/1‑150 (MQ=255)

                                                                tAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTcc                                                                                   <  2:2845221/150‑1 (MQ=255)

                                                                   cGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCt                                                                                <  1:651718/150‑1 (MQ=255)

                                                                      cTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTgcg                                                                             <  1:1885177/150‑1 (MQ=255)

                                                                         gacgacAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCtt                                                                          <  2:2343879/150‑1 (MQ=255)

                                                                              cAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCt                                                                     >  2:1302044/1‑150 (MQ=255)

                                                                              cAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCt                                                                     <  1:1307984/150‑1 (MQ=255)

                                                                                    gTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATact                                                               <  2:502276/150‑1 (MQ=255)

                                                                                       ttGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATactgag                                                            <  1:2417909/150‑1 (MQ=255)

                                                                                        tGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATactgaga                                                           >  1:183364/1‑150 (MQ=255)

                                                                                          ggCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATactgagact                                                         <  2:607589/150‑1 (MQ=255)

                                                                                             tACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATactgagactgag                                                      <  1:1056857/150‑1 (MQ=255)

                                                                                               cGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCg                                                    >  1:1561561/1‑150 (MQ=255)

                                                                                               cGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCg                                                    <  1:3426876/150‑1 (MQ=255)

                                                                                                gCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGt                                                   <  1:3961788/150‑1 (MQ=255)

                                                                                                 ccGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTc                                                  >  1:1464799/1‑150 (MQ=255)

                                                                                                   ggAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGa                                                >  2:4574931/1‑150 (MQ=255)

                                                                                                    gAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGAt                                               >  2:4220932/1‑150 (MQ=255)

                                                                                                      aaaTTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCACTCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTc                                             >  2:4515942/1‑150 (MQ=255)

                                                                                                      aaaTTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTc                                             >  2:3090216/1‑150 (MQ=255)

                                                                                                       aaTTTGCCAACAATTTACCTCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCa                                            <  2:1705219/150‑1 (MQ=255)

                                                                                                        aTTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCAc                                           >  2:202252/1‑150 (MQ=255)

                                                                                                            gCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGc                                       >  1:1950276/1‑150 (MQ=255)

                                                                                                              caacaaTTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCaa                                     >  1:887694/1‑150 (MQ=255)

                                                                                                              caacaaTTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCaa                                     >  1:3952187/1‑150 (MQ=255)

                                                                                                               aacaaTTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCaaa                                    >  2:3783788/1‑150 (MQ=255)

                                                                                                                acaaTTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAAc                                   <  2:1459766/150‑1 (MQ=255)

                                                                                                                acaaTTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTAGACTGAGACTGAGCGTCGATTCACATGCAAAc                                   <  2:196612/150‑1 (MQ=255)

                                                                                                                 caaTTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACg                                  <  1:2102096/150‑1 (MQ=255)

                                                                                                                 caaTTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACg                                  <  2:3037478/150‑1 (MQ=255)

                                                                                                                  aaTTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACgg                                 >  2:1165356/1‑150 (MQ=255)

                                                                                                                    tttACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGgcg                               <  1:444941/150‑1 (MQ=255)

                                                                                                                    tttACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGgcg                               <  1:4517996/150‑1 (MQ=255)

                                                                                                                     ttACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGgcgc                              >  2:392004/1‑150 (MQ=255)

                                                                                                                        ccGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCAtt                           >  2:3814258/1‑150 (MQ=255)

                                                                                                                         cGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCAttt                          <  2:3796324/150‑1 (MQ=255)

                                                                                                                         cGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCAttt                          <  2:1602827/150‑1 (MQ=255)

                                                                                                                          gCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCAtttt                         >  1:3057236/1‑150 (MQ=255)

                                                                                                                           cAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCAttttt                        >  2:3760653/1‑150 (MQ=255)

                                                                                                                            aaGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTa                       >  2:934130/1‑150 (MQ=255)

                                                                                                                            aaGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTa                       >  2:3950386/1‑150 (MQ=255)

                                                                                                                            aaGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTa                       <  1:3229476/150‑1 (MQ=255)

                                                                                                                            aaGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTa                       >  2:3247933/1‑150 (MQ=255)

                                                                                                                                 gcgcgTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGaata                  <  2:2376958/150‑1 (MQ=255)

                                                                                                                                   gcgTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGaataat                <  2:2499383/150‑1 (MQ=255)

                                                                                                                                    cgTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATc               <  1:3678855/150‑1 (MQ=255)

                                                                                                                                     gTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATcc              >  1:3352489/1‑150 (MQ=255)

                                                                                                                                      tCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCt             <  2:179194/150‑1 (MQ=255)

                                                                                                                                        aTGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGa           >  2:2787332/1‑150 (MQ=255)

                                                                                                                                        aTGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGa           <  1:2016445/150‑1 (MQ=255)

                                                                                                                                          gTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGAcc         >  1:4363592/1‑150 (MQ=255)

                                                                                                                                           tACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCt        <  1:3269814/150‑1 (MQ=255)

                                                                                                                                            aCATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCtt       >  1:2057805/1‑150 (MQ=255)

                                                                                                                                             cATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTtg      >  1:4005785/1‑150 (MQ=255)

                                                                                                                                             cATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTtg      >  1:797151/1‑150 (MQ=255)

                                                                                                                                              aTGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTtgt     <  2:41771/150‑1 (MQ=255)

                                                                                                                                               tGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTtgtg    <  1:643422/150‑1 (MQ=255)

                                                                                                                                                 gAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTGCg  >  2:672149/1‑150 (MQ=255)

                                                                                                                                                 gAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTGCg  >  1:1074244/1‑150 (MQ=255)

GCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTGCG  >  CP009273/3074966‑3075260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTGCGGAA  >  CP009273/3074965‑3075263

AGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAA                                                                                                                                                       <  SRR19973073.570472/150‑1 (MQ=60)

 GCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACA                                                                                                                                                      <  SRR19973073.1162125/150‑1 (MQ=60)

  CATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAAT                                                                                                                                                     <  SRR19973073.1650194/150‑1 (MQ=60)

  CATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAAT                                                                                                                                                     <  SRR19973073.1650345/150‑1 (MQ=60)

  CATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAAT                                                                                                                                                     >  SRR19973073.3255257/1‑150 (MQ=60)

      GGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTT                                                                                                                                                 >  SRR19973073.4055670/1‑150 (MQ=60)

      GGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTT                                                                                                                                                 >  SRR19973073.4055675/1‑150 (MQ=60)

       GCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTT                                                                                                                                                <  SRR19973073.2937642/150‑1 (MQ=60)

       GCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTT                                                                                                                                                <  SRR19973073.1211834/150‑1 (MQ=60)

          AGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGC                                                                                                                                             >  SRR19973073.2638577/1‑150 (MQ=60)

          AGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGC                                                                                                                                             >  SRR19973073.4238012/1‑150 (MQ=60)

             TCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCGCGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGC                                                                                                                                          >  SRR19973073.860195/1‑150 (MQ=60)

             TCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGC                                                                                                                                          >  SRR19973073.1325096/1‑150 (MQ=60)

              CTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCT                                                                                                                                         >  SRR19973073.3402266/1‑150 (MQ=60)

              CTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCT                                                                                                                                         >  SRR19973073.3402268/1‑150 (MQ=60)

               TTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTT                                                                                                                                        <  SRR19973073.3134988/150‑1 (MQ=60)

               TTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTT                                                                                                                                        <  SRR19973073.4397966/150‑1 (MQ=60)

                   CGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGA                                                                                                                                    >  SRR19973073.1213157/1‑150 (MQ=60)

                    GTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAA                                                                                                                                   >  SRR19973073.472242/1‑150 (MQ=60)

                     TGAGGACATTTTGACTCCAGATCGGAGGATGAAGGGCTCGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAA                                                                                                                                  <  SRR19973073.1337830/150‑1 (MQ=60)

                      GAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAAT                                                                                                                                 >  SRR19973073.2096272/1‑150 (MQ=60)

                      GAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAAT                                                                                                                                 <  SRR19973073.434143/150‑1 (MQ=60)

                          ACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCT                                                                                                                             <  SRR19973073.1183953/150‑1 (MQ=60)

                              TTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGAT                                                                                                                         <  SRR19973073.3769390/150‑1 (MQ=60)

                               TTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATC                                                                                                                        <  SRR19973073.1305011/150‑1 (MQ=60)

                               TTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATC                                                                                                                        <  SRR19973073.1304952/150‑1 (MQ=60)

                               TTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATC                                                                                                                        <  SRR19973073.1304942/150‑1 (MQ=60)

                                 GACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCAT                                                                                                                      <  SRR19973073.3780466/150‑1 (MQ=60)

                                 GACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCAT                                                                                                                      <  SRR19973073.2462900/150‑1 (MQ=60)

                                  ACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATG                                                                                                                     >  SRR19973073.310946/1‑150 (MQ=60)

                                  ACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATG                                                                                                                     >  SRR19973073.310945/1‑150 (MQ=60)

                                  ACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATG                                                                                                                     >  SRR19973073.310943/1‑150 (MQ=60)

                                  ACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATG                                                                                                                     >  SRR19973073.989942/1‑150 (MQ=60)

                                  ACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATG                                                                                                                     >  SRR19973073.990151/1‑150 (MQ=60)

                                   CTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGATATCTCTGGATCATGC                                                                                                                    >  SRR19973073.4216818/1‑150 (MQ=60)

                                   CTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGC                                                                                                                    >  SRR19973073.1711864/1‑150 (MQ=60)

                                   CTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGC                                                                                                                    >  SRR19973073.2300895/1‑150 (MQ=60)

                                    TCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCT                                                                                                                   <  SRR19973073.3303383/150‑1 (MQ=60)

                                      CAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCG                                                                                                                 >  SRR19973073.1863656/1‑150 (MQ=60)

                                      CAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCG                                                                                                                 >  SRR19973073.1863657/1‑150 (MQ=60)

                                      CAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCG                                                                                                                 >  SRR19973073.1863662/1‑150 (MQ=60)

                                       AGATCGGATGATGAAGGGCACGCCCTTAACTACTTGACGACAGCGCGATTTGGTCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGA                                                                                                                >  SRR19973073.1981471/1‑150 (MQ=60)

                                       AGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGC                                                                                                                >  SRR19973073.3421967/1‑150 (MQ=60)

                                       AGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGC                                                                                                                >  SRR19973073.1981482/1‑150 (MQ=60)

                                        GATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCA                                                                                                               >  SRR19973073.409824/1‑150 (MQ=60)

                                           CGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGACAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGT                                                                                                            >  SRR19973073.1509868/1‑150 (MQ=60)

                                             GATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTG                                                                                                          <  SRR19973073.1929003/150‑1 (MQ=60)

                                               TGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCG                                                                                                        <  SRR19973073.4277684/150‑1 (MQ=60)

                                                 ATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCA                                                                                                      >  SRR19973073.1757876/1‑150 (MQ=60)

                                                  TGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAA                                                                                                     >  SRR19973073.3780079/1‑150 (MQ=60)

                                                    AAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATC                                                                                                   >  SRR19973073.910553/1‑150 (MQ=60)

                                                       GGCGCGCCCTTAACGACTGGACGACAGCGCGGTTTGGGCTACGCCGGAAAATTTGCCAACAATTGACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTGGCCGCTTCGGACATCTCTGGATCATGCTCGGAGGTTGCGCAATCTAC                                                                                                >  SRR19973073.1979824/1‑150 (MQ=60)

                                                          ACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCG                                                                                             <  SRR19973073.1263419/150‑1 (MQ=60)

                                                           CGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGC                                                                                            >  SRR19973073.1861991/1‑150 (MQ=60)

                                                             CCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCC                                                                                          <  SRR19973073.1990883/150‑1 (MQ=60)

                                                             CCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCC                                                                                          >  SRR19973073.634441/1‑150 (MQ=60)

                                                                 TAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCC                                                                                      >  SRR19973073.3713387/1‑150 (MQ=60)

                                                                 TAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCC                                                                                      <  SRR19973073.3342711/150‑1 (MQ=60)

                                                                 TAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCC                                                                                      <  SRR19973073.1596983/150‑1 (MQ=60)

                                                                 TAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCC                                                                                      >  SRR19973073.1169033/1‑150 (MQ=60)

                                                                    CGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCT                                                                                   <  SRR19973073.653093/150‑1 (MQ=60)

                                                                    CGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCT                                                                                   <  SRR19973073.652981/150‑1 (MQ=60)

                                                                    CGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCT                                                                                   <  SRR19973073.652982/150‑1 (MQ=60)

                                                                    CGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCT                                                                                   <  SRR19973073.2463452/150‑1 (MQ=60)

                                                                     GACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTG                                                                                  <  SRR19973073.3189227/150‑1 (MQ=60)

                                                                       CTTGACGTCAGAGCGTTTTGGGCTACGCCGGAAAATTTGCCTACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCATTAATCTCTGGTTCATGATCGCATGTTGAGCAATCTTCTCGCCCGTCCGCTGCG                                                                                <  SRR19973073.1888305/150‑1 (MQ=60)

                                                                       CTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCG                                                                                <  SRR19973073.1889027/150‑1 (MQ=60)

                                                                          GACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTT                                                                             >  SRR19973073.4307119/1‑150 (MQ=60)

                                                                               CAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCT                                                                        <  SRR19973073.1310752/150‑1 (MQ=60)

                                                                                  CGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTAT                                                                     <  SRR19973073.309046/150‑1 (MQ=60)

                                                                                   GCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATA                                                                    <  SRR19973073.4565413/150‑1 (MQ=60)

                                                                                   GCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATA                                                                    >  SRR19973073.3260815/1‑150 (MQ=60)

                                                                                     GTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACT                                                                  <  SRR19973073.3780888/150‑1 (MQ=60)

                                                                                        TTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAG                                                               <  SRR19973073.2422888/150‑1 (MQ=60)

                                                                                         TGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGA                                                              >  SRR19973073.183798/1‑150 (MQ=60)

                                                                                           GGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACT                                                            <  SRR19973073.2270157/150‑1 (MQ=60)

                                                                                             CTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGA                                                          <  SRR19973073.725265/150‑1 (MQ=60)

                                                                                             CTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGA                                                          <  SRR19973073.2046610/150‑1 (MQ=60)

                                                                                              TACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAG                                                         <  SRR19973073.1059242/150‑1 (MQ=60)

                                                                                              TACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAG                                                         <  SRR19973073.1059052/150‑1 (MQ=60)

                                                                                                CGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCG                                                       <  SRR19973073.2977996/150‑1 (MQ=60)

                                                                                                CGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCG                                                       <  SRR19973073.3433720/150‑1 (MQ=60)

                                                                                                CGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCG                                                       >  SRR19973073.1564755/1‑150 (MQ=60)

                                                                                                 GCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGT                                                      <  SRR19973073.3969678/150‑1 (MQ=60)

                                                                                                 GCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGT                                                      <  SRR19973073.3969658/150‑1 (MQ=60)

                                                                                                  CCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTC                                                     >  SRR19973073.1467867/1‑150 (MQ=60)

                                                                                                  CCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTC                                                     <  SRR19973073.1501208/150‑1 (MQ=60)

                                                                                                             GCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGC                                          >  SRR19973073.1954277/1‑150 (MQ=60)

                                                                                                               CAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAA                                        >  SRR19973073.3960055/1‑150 (MQ=60)

                                                                                                               CAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAA                                        >  SRR19973073.889525/1‑150 (MQ=60)

                                                                                                               CAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAA                                        >  SRR19973073.681475/1‑150 (MQ=60)

                                                                                                                AACAATTTACCGCAAGCCGCGCGTCATGTACATTGAAAATCCTTTTGCCGCTTCTTAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAA                                       <  SRR19973073.4576543/150‑1 (MQ=60)

                                                                                                                  CAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACG                                     <  SRR19973073.2106493/150‑1 (MQ=60)

                                                                                                                     TTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCG                                  >  SRR19973073.1688923/1‑150 (MQ=60)

                                                                                                                     TTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCG                                  <  SRR19973073.4527042/150‑1 (MQ=60)

                                                                                                                     TTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCG                                  <  SRR19973073.445852/150‑1 (MQ=60)

                                                                                                                     TTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCG                                  <  SRR19973073.4527065/150‑1 (MQ=60)

                                                                                                                      TTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGC                                 >  SRR19973073.191262/1‑150 (MQ=60)

                                                                                                                      TTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGC                                 >  SRR19973073.2508123/1‑150 (MQ=60)

                                                                                                                           GCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTT                            >  SRR19973073.3063397/1‑150 (MQ=60)

                                                                                                                             AAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTA                          <  SRR19973073.3235992/150‑1 (MQ=60)

                                                                                                                              AGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAG                         <  SRR19973073.1281848/150‑1 (MQ=60)

                                                                                                                                CCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAA                       >  SRR19973073.2456304/1‑150 (MQ=60)

                                                                                                                                    GCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAAT                   <  SRR19973073.4405054/150‑1 (MQ=60)

                                                                                                                                    GCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAAT                   <  SRR19973073.4326252/150‑1 (MQ=60)

                                                                                                                                     CGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATC                  <  SRR19973073.4346942/150‑1 (MQ=60)

                                                                                                                                     CGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATC                  <  SRR19973073.3686236/150‑1 (MQ=60)

                                                                                                                                     CGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATC                  <  SRR19973073.2043525/150‑1 (MQ=60)

                                                                                                                                     CGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTACGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATC                  <  SRR19973073.3986124/150‑1 (MQ=60)

                                                                                                                                      GTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCC                 >  SRR19973073.178292/1‑150 (MQ=60)

                                                                                                                                      GTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCC                 >  SRR19973073.3359256/1‑150 (MQ=60)

                                                                                                                                        CATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTG               >  SRR19973073.3197383/1‑150 (MQ=60)

                                                                                                                                         ATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGA              <  SRR19973073.2020641/150‑1 (MQ=60)

                                                                                                                                         ATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATATTTAGAATAATCCTGA              >  SRR19973073.3392984/1‑150 (MQ=60)

                                                                                                                                           GTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACC            >  SRR19973073.4372336/1‑150 (MQ=60)

                                                                                                                                            TACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCT           <  SRR19973073.3276437/150‑1 (MQ=60)

                                                                                                                                             ACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTT          >  SRR19973073.2062113/1‑150 (MQ=60)

                                                                                                                                             ACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTT          >  SRR19973073.2062088/1‑150 (MQ=60)

                                                                                                                                              CATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTG         >  SRR19973073.4013562/1‑150 (MQ=60)

                                                                                                                                              CATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTG         >  SRR19973073.4013781/1‑150 (MQ=60)

                                                                                                                                              CATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTG         >  SRR19973073.798764/1‑150 (MQ=60)

                                                                                                                                              CATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTG         >  SRR19973073.798922/1‑150 (MQ=60)

                                                                                                                                              CATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTG         <  SRR19973073.3477879/150‑1 (MQ=60)

                                                                                                                                                TGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTG       <  SRR19973073.644786/150‑1 (MQ=60)

                                                                                                                                                TGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTG       <  SRR19973073.4490520/150‑1 (MQ=60)

                                                                                                                                                TGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTG       <  SRR19973073.3657923/150‑1 (MQ=60)

                                                                                                                                                  GAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTGCG     >  SRR19973073.1076463/1‑150 (MQ=60)

                                                                                                                                                     AATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTGCGGAA  <  SRR19973073.2781668/150‑1 (MQ=60)

AGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTGCGGAA  >  CP009273/3074965‑3075263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`