Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F434 I0 R1	1130	215.9	7031662	97.1%	6827743	147.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,075,114	C→A	100%	intergenic (‑120/‑158)	tktA ← / → loiP	transketolase 1, thiamine triphosphate‑binding/Phe‑Phe periplasmic metalloprotease, OM lipoprotein; low salt‑inducible; heat shock protein that binds Era

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,075,114	0	C	A	99.5%	693.9 / ‑4.3	211	intergenic (‑120/‑158)	tktA/loiP	transketolase 1, thiamine triphosphate‑binding/Phe‑Phe periplasmic metalloprotease, OM lipoprotein; low salt‑inducible; heat shock protein that binds Era
Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (94/116); total (94/117)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 211 total aligned reads displayed.

TTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTG  >  CP009273/3074969‑3075258

ttGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAAt                                                                                                                                                >  2:1044027/1‑148 (MQ=255)

ttGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAAt                                                                                                                                                >  2:1574240/1‑148 (MQ=255)

ttGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAAt                                                                                                                                                >  2:3359184/1‑148 (MQ=255)

ttGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAAt                                                                                                                                                >  2:2796695/1‑148 (MQ=255)

 tGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATc                                                                                                                                               >  1:2125517/1‑148 (MQ=255)

 tGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATc                                                                                                                                               <  1:2113054/148‑1 (MQ=255)

    cAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCtt                                                                                                                                            <  2:510077/148‑1 (MQ=255)

    cAAGCTCTTTACGTGAGGACATTTTGACTCCAGAGCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCtt                                                                                                                                            <  2:2319941/148‑1 (MQ=255)

      aGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCtttt                                                                                                                                          >  1:474727/1‑148 (MQ=255)

       gCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTg                                                                                                                                         >  1:2289049/1‑148 (MQ=255)

        ctctTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGc                                                                                                                                        >  1:378877/1‑148 (MQ=255)

        ctctTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGc                                                                                                                                        >  1:170091/1‑148 (MQ=255)

          ctTTACGTTTGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCg                                                                                                                                      <  1:2810335/148‑1 (MQ=255)

          ctTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCtt                                                                                                                                            <  2:1515887/142‑1 (MQ=255)

          ctTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCtt                                                                                                                                            >  1:1515887/1‑142 (MQ=255)

          ctTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCg                                                                                                                                      <  2:2791807/148‑1 (MQ=255)

           tttACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGc                                                                                                                                     >  1:3152335/1‑148 (MQ=255)

             tACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCtt                                                                                                                                   <  1:1481814/148‑1 (MQ=255)

             tACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCtt                                                                                                                                   <  1:1057785/148‑1 (MQ=255)

             tACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCtt                                                                                                                                   <  1:1088479/148‑1 (MQ=255)

                  gAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGaa                                                                                                                              <  1:3430427/148‑1 (MQ=255)

                     gACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAAtc                                                                                                                           >  1:1625310/1‑148 (MQ=255)

                       cATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAAtctc                                                                                                                         <  1:2777650/148‑1 (MQ=255)

                       cATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAAtctc                                                                                                                         <  1:1017342/148‑1 (MQ=255)

                       cATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAAtctc                                                                                                                         >  1:3374463/1‑148 (MQ=255)

                       cATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAAtctc                                                                                                                         <  1:1796760/148‑1 (MQ=255)

                         ttttGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAAtctcgg                                                                                                                       >  2:2805483/1‑146 (MQ=255)

                         ttttGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTg                                                                                                                       <  1:2957820/148‑1 (MQ=255)

                         ttttGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTg                                                                                                                       >  2:1287296/1‑148 (MQ=255)

                          tttGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTgg                                                                                                                      <  1:178782/148‑1 (MQ=255)

                              aCTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCa                                                                                                                  >  1:270968/1‑148 (MQ=255)

                                tCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGAGCGTTTTGTGCTACGCCGGAAAATTTGACAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATg                                                                                                                >  2:3232123/1‑148 (MQ=255)

                                tCCAGATCGGAGGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGGGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATg                                                                                                                <  2:1827512/148‑1 (MQ=255)

                                 ccAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGACAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGc                                                                                                               >  1:2055690/1‑148 (MQ=255)

                                  cAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCt                                                                                                              <  1:584782/148‑1 (MQ=255)

                                   aGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTc                                                                                                             >  2:2221170/1‑148 (MQ=255)

                                   aGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTc                                                                                                             >  2:2986960/1‑148 (MQ=255)

                                     aTCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGc                                                                                                           >  2:2889661/1‑148 (MQ=255)

                                          atgatgAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGtt                                                                                                      >  1:1441688/1‑148 (MQ=255)

                                               gaAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCa                                                                                                 >  2:1126138/1‑148 (MQ=255)

                                                aaGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCaa                                                                                                <  2:678142/148‑1 (MQ=255)

                                                       cGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCa                                                                                                          <  2:1899938/131‑1 (MQ=255)

                                                       cGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCa                                                                                                          >  1:1899938/1‑131 (MQ=255)

                                                       cGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTc                                                                                         >  1:1015224/1‑148 (MQ=255)

                                                       cGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTc                                                                                         >  2:2965917/1‑148 (MQ=255)

                                                          ccTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGcc                                                                                      >  2:2162218/1‑148 (MQ=255)

                                                          ccTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGcc                                                                                      <  2:2897714/148‑1 (MQ=255)

                                                           cTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGccc                                                                                     >  2:777111/1‑148 (MQ=255)

                                                            ttAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCGAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCg                                                                                    >  2:770616/1‑148 (MQ=255)

                                                            ttAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCg                                                                                    >  1:1408562/1‑148 (MQ=255)

                                                            ttAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCg                                                                                    >  1:2096928/1‑148 (MQ=255)

                                                               aCGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTcc                                                                                 >  1:2662791/1‑148 (MQ=255)

                                                                 gACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGc                                                                               <  1:2763042/148‑1 (MQ=255)

                                                                     tGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACACTTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTgcg                                                                           <  1:3359458/148‑1 (MQ=255)

                                                                     tGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTgcg                                                                           >  2:560560/1‑148 (MQ=255)

                                                                     tGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTgcg                                                                           >  2:3174991/1‑148 (MQ=255)

                                                                     tGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTgcg                                                                           >  2:3255610/1‑148 (MQ=255)

                                                                       acgacAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCt                                                                         <  1:1226198/148‑1 (MQ=255)

                                                                        cgacAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCtt                                                                        >  1:1088984/1‑148 (MQ=255)

                                                                           cAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGTGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTc                                                                     <  2:442860/148‑1 (MQ=255)

                                                                           cAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTc                                                                     <  2:3108403/148‑1 (MQ=255)

                                                                           cAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTc                                                                     <  2:1408562/148‑1 (MQ=255)

                                                                           cAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTc                                                                     <  2:2097367/148‑1 (MQ=255)

                                                                            aGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTAAATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTcc                                                                    <  1:2271704/148‑1 (MQ=255)

                                                                             gcgcGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCt                                                                   >  1:1923072/1‑148 (MQ=255)

                                                                                cgTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTtat                                                                <  2:1352588/148‑1 (MQ=255)

                                                                                 gTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTtata                                                               <  2:1604135/148‑1 (MQ=255)

                                                                                  ttttGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATac                                                              >  2:2000158/1‑148 (MQ=255)

                                                                                  ttttGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATac                                                              <  1:718660/148‑1 (MQ=255)

                                                                                     tGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATactga                                                           <  2:3311131/148‑1 (MQ=255)

                                                                                       ggCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATactgaga                                                         <  2:3374463/148‑1 (MQ=255)

                                                                                        gCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATactgagac                                                        <  1:815447/148‑1 (MQ=255)

                                                                                         cTACGCCGGAAAATTTGACAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATactgagact                                                       <  2:402063/148‑1 (MQ=255)

                                                                                          tACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATactgagactg                                                      <  1:1367498/148‑1 (MQ=255)

                                                                                            cGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATactgagactgag                                                    <  2:70664/148‑1 (MQ=255)

                                                                                             gCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGc                                                   <  1:1001998/148‑1 (MQ=255)

                                                                                              ccGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCg                                                  <  1:3019838/148‑1 (MQ=255)

                                                                                                  aaaaTTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGa                                              >  1:615261/1‑148 (MQ=255)

                                                                                                  aaaaTTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGa                                              <  1:2652948/148‑1 (MQ=255)

                                                                                                     aTTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTc                                           >  2:601149/1‑148 (MQ=255)

                                                                                                            aacaaTTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCa                                    >  2:2063942/1‑148 (MQ=255)

                                                                                                                aTTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACg                                <  1:1309382/148‑1 (MQ=255)

                                                                                                                aTTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACg                                >  1:3026750/1‑148 (MQ=255)

                                                                                                                   tACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGgcg                             <  1:2162218/148‑1 (MQ=255)

                                                                                                                     ccGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCa                           <  1:1563491/148‑1 (MQ=255)

                                                                                                                     ccGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCa                           <  1:876678/148‑1 (MQ=255)

                                                                                                                       gCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCAtt                         <  1:2260321/148‑1 (MQ=255)

                                                                                                                        cAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCAttt                        <  2:3341957/148‑1 (MQ=255)

                                                                                                                           gCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTa                     >  1:861326/1‑148 (MQ=255)

                                                                                                                            ccGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAg                    >  2:1988956/1‑148 (MQ=255)

                                                                                                                              gcgcgTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGaa                  <  1:2965917/148‑1 (MQ=255)

                                                                                                                                gcgTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGaata                <  2:2563776/148‑1 (MQ=255)

                                                                                                                                 cgTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGaataa               >  1:845091/1‑148 (MQ=255)

                                                                                                                                 cgTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCGGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGaataa               <  1:2805080/148‑1 (MQ=255)

                                                                                                                                   tCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATc             >  1:2082830/1‑148 (MQ=255)

                                                                                                                                     aTGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCt           >  1:1585562/1‑148 (MQ=255)

                                                                                                                                        tACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGAc        <  1:3241677/148‑1 (MQ=255)

                                                                                                                                          cATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCt      <  2:2927241/148‑1 (MQ=255)

                                                                                                                                            tGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTtg    >  2:3436951/1‑148 (MQ=255)

                                                                                                                                              gAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTtgtg  <  2:2154706/148‑1 (MQ=255)

TTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTG  >  CP009273/3074969‑3075258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTG  >  CP009273/3074967‑3075258

CATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAAT                                                                                                                                                <  SRR19973065.597579/150‑1 (MQ=60)

CATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAAT                                                                                                                                                >  SRR19973065.1196248/1‑150 (MQ=60)

 ATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATC                                                                                                                                               >  SRR19973065.2139198/1‑150 (MQ=60)

   TGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCT                                                                                                                                             <  SRR19973065.2126824/150‑1 (MQ=60)

   TGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCT                                                                                                                                             <  SRR19973065.2126654/150‑1 (MQ=60)

   TGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCT                                                                                                                                             <  SRR19973065.741588/150‑1 (MQ=60)

      CAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTT                                                                                                                                          <  SRR19973065.1721746/150‑1 (MQ=60)

      CAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTT                                                                                                                                          >  SRR19973065.477969/1‑150 (MQ=60)

       AAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGTATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTG                                                                                                                                         <  SRR19973065.607791/150‑1 (MQ=60)

       AAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTG                                                                                                                                         >  SRR19973065.2303469/1‑150 (MQ=60)

        AGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGC                                                                                                                                        >  SRR19973065.171461/1‑150 (MQ=60)

        AGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGC                                                                                                                                        >  SRR19973065.381523/1‑150 (MQ=60)

          CTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTagat                                                                                                                                      >  SRR19973065.1525793/1‑146 (MQ=60)

           TCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGC                                                                                                                                     >  SRR19973065.1865987/1‑150 (MQ=60)

           TCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGC                                                                                                                                     >  SRR19973065.3171460/1‑150 (MQ=60)

            CTTTACGTTTGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCT                                                                                                                                    <  SRR19973065.2827716/150‑1 (MQ=60)

               TACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCA                                                                                                                                 <  SRR19973065.310152/150‑1 (MQ=60)

               TACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCA                                                                                                                                 <  SRR19973065.1095652/150‑1 (MQ=60)

               TACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCA                                                                                                                                 <  SRR19973065.1064794/150‑1 (MQ=60)

               TACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCA                                                                                                                                 <  SRR19973065.1491136/150‑1 (MQ=60)

               TACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCA                                                                                                                                 <  SRR19973065.1491277/150‑1 (MQ=60)

                    GAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAAT                                                                                                                            <  SRR19973065.3451357/150‑1 (MQ=60)

                    GAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAAT                                                                                                                            <  SRR19973065.1644124/150‑1 (MQ=60)

                     AGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATC                                                                                                                           >  SRR19973065.1636204/1‑150 (MQ=60)

                     AGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATC                                                                                                                           >  SRR19973065.1636185/1‑150 (MQ=60)

                      GGACATTTTGCCTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCT                                                                                                                          <  SRR19973065.1012891/150‑1 (MQ=60)

                      GGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCT                                                                                                                          <  SRR19973065.2776079/150‑1 (MQ=60)

                       GACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTC                                                                                                                         >  SRR19973065.3394884/1‑150 (MQ=60)

                       GACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTC                                                                                                                         >  SRR19973065.1426139/1‑150 (MQ=60)

                       GACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTC                                                                                                                         >  SRR19973065.3395100/1‑150 (MQ=60)

                         CATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTG                                                                                                                       <  SRR19973065.1023492/150‑1 (MQ=60)

                         CATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTG                                                                                                                       <  SRR19973065.1024084/150‑1 (MQ=60)

                         CATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTG                                                                                                                       <  SRR19973065.2794889/150‑1 (MQ=60)

                         CATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTG                                                                                                                       <  SRR19973065.1808563/150‑1 (MQ=60)

                         CATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTG                                                                                                                       <  SRR19973065.1808499/150‑1 (MQ=60)

                         CATTTTGACCCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTG                                                                                                                       <  SRR19973065.2209925/150‑1 (MQ=60)

                           TTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGA                                                                                                                     <  SRR19973065.2975878/150‑1 (MQ=60)

                            TTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGAT                                                                                                                    <  SRR19973065.180194/150‑1 (MQ=60)

                             TTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGACATCTCTGGATC                                                                                                                   >  SRR19973065.259514/1‑150 (MQ=60)

                             TTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATC                                                                                                                   >  SRR19973065.916942/1‑150 (MQ=60)

                              TGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCA                                                                                                                  >  SRR19973065.272800/1‑150 (MQ=60)

                                 CTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGACAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGC                                                                                                               >  SRR19973065.2068817/1‑150 (MQ=60)

                                 CTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGC                                                                                                               >  SRR19973065.2976749/1‑150 (MQ=60)

                                 CTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGC                                                                                                               <  SRR19973065.3448161/150‑1 (MQ=60)

                                   CCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTC                                                                                                             <  SRR19973065.1372098/150‑1 (MQ=60)

                                    CAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCG                                                                                                            <  SRR19973065.588423/150‑1 (MQ=60)

                                      GATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCA                                                                                                          <  SRR19973065.1895990/150‑1 (MQ=60)

                                          GGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTT                                                                                                      >  SRR19973065.1451026/1‑150 (MQ=60)

                                                TGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAA                                                                                                >  SRR19973065.2907013/1‑150 (MQ=60)

                                                       CACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGagatcggaagagcac                                                                                         >  SRR19973065.1912422/1‑135 (MQ=60)

                                                       CACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTC                                                                                         >  SRR19973065.1021951/1‑150 (MQ=60)

                                                            CCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCG                                                                                    >  SRR19973065.1417778/1‑150 (MQ=60)

                                                            CCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCG                                                                                    >  SRR19973065.2110413/1‑150 (MQ=60)

                                                            CCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCG                                                                                    >  SRR19973065.2110858/1‑150 (MQ=60)

                                                             CTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGT                                                                                   >  SRR19973065.1572806/1‑150 (MQ=60)

                                                               TAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCC                                                                                 >  SRR19973065.2679269/1‑150 (MQ=60)

                                                               TAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCC                                                                                 >  SRR19973065.2679265/1‑150 (MQ=60)

                                                                   GACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTG                                                                             <  SRR19973065.2780209/150‑1 (MQ=60)

                                                                       TGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACACTTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCT                                                                         <  SRR19973065.3379999/150‑1 (MQ=60)

                                                                        GACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTT                                                                        >  SRR19973065.2280313/1‑150 (MQ=60)

                                                                        GACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTT                                                                        >  SRR19973065.503306/1‑150 (MQ=60)

                                                                        GACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTT                                                                        >  SRR19973065.1096161/1‑150 (MQ=60)

                                                                        GACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTT                                                                        >  SRR19973065.1095791/1‑150 (MQ=60)

                                                                         ACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTT                                                                       <  SRR19973065.426193/150‑1 (MQ=60)

                                                                         ACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTT                                                                       <  SRR19973065.1234197/150‑1 (MQ=60)

                                                                             CAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCT                                                                   >  SRR19973065.1935680/1‑150 (MQ=60)

                                                                             CAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCAT                                                                   >  SRR19973065.1935679/1‑150 (MQ=60)

                                                                              AGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTT                                                                  <  SRR19973065.1050927/150‑1 (MQ=60)

                                                                              AGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTT                                                                  <  SRR19973065.2814021/150‑1 (MQ=60)

                                                                              AGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTT                                                                  <  SRR19973065.1584719/150‑1 (MQ=60)

                                                                              AGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTAAATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTT                                                                  <  SRR19973065.2286049/150‑1 (MQ=60)

                                                                                CGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTAT                                                                <  SRR19973065.3519015/150‑1 (MQ=60)

                                                                                CGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTAT                                                                <  SRR19973065.3519544/150‑1 (MQ=60)

                                                                                    TTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTG                                                            <  SRR19973065.653816/150‑1 (MQ=60)

                                                                                    TTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTG                                                            <  SRR19973065.723469/150‑1 (MQ=60)

                                                                                          GCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTG                                                      <  SRR19973065.820817/150‑1 (MQ=60)

                                                                                            TACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAG                                                    <  SRR19973065.1376534/150‑1 (MQ=60)

                                                                                            TACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAG                                                    <  SRR19973065.1376542/150‑1 (MQ=60)

                                                                                               GCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGT                                                 <  SRR19973065.1008653/150‑1 (MQ=60)

                                                                                                CCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTC                                                <  SRR19973065.3038339/150‑1 (MQ=60)

                                                                                                CCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTC                                                <  SRR19973065.3038675/150‑1 (MQ=60)

                                                                                                  GGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGA                                              >  SRR19973065.619205/1‑150 (MQ=60)

                                                                                                    AAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATT                                            <  SRR19973065.2669331/150‑1 (MQ=60)

                                                                                                     AAATTTGCCAACTATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTC                                           <  SRR19973065.2969455/150‑1 (MQ=60)

                                                                                                               ACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAAC                                 >  SRR19973065.2301400/1‑150 (MQ=60)

                                                                                                                CAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACG                                >  SRR19973065.3045299/1‑150 (MQ=60)

                                                                                                                  ATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGC                              <  SRR19973065.1318027/150‑1 (MQ=60)

                                                                                                                  ATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGC                              >  SRR19973065.3299781/1‑150 (MQ=60)

                                                                                                                     TACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCA                           <  SRR19973065.2175658/150‑1 (MQ=60)

                                                                                                                     TACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCGGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCA                           <  SRR19973065.2176115/150‑1 (MQ=60)

                                                                                                                       CCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATT                         <  SRR19973065.882275/150‑1 (MQ=60)

                                                                                                                       CCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATT                         <  SRR19973065.1573881/150‑1 (MQ=60)

                                                                                                                       CCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATT                         <  SRR19973065.881818/150‑1 (MQ=60)

                                                                                                                         GCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCATAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCCCCTGCAAACGGCGCATTTT                       <  SRR19973065.2714322/150‑1 (MQ=60)

                                                                                                                         GCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTT                       <  SRR19973065.2039951/150‑1 (MQ=60)

                                                                                                                         GCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTT                       <  SRR19973065.2274615/150‑1 (MQ=60)

                                                                                                                         GCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTT                       <  SRR19973065.1287200/150‑1 (MQ=60)

                                                                                                                         GCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTT                       <  SRR19973065.2274704/150‑1 (MQ=60)

                                                                                                                           AAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTA                     >  SRR19973065.866861/1‑150 (MQ=60)

                                                                                                                           AAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTA                     >  SRR19973065.144369/1‑150 (MQ=60)

                                                                                                                           AAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTA                     >  SRR19973065.866873/1‑150 (MQ=60)

                                                                                                                                GCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATA                <  SRR19973065.2984054/150‑1 (MQ=60)

                                                                                                                                GCGCGTAATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATA                <  SRR19973065.2984051/150‑1 (MQ=60)

                                                                                                                                 CGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAA               >  SRR19973065.850434/1‑150 (MQ=60)

                                                                                                                                 CGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAA               >  SRR19973065.850577/1‑150 (MQ=60)

                                                                                                                                   CGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATC             >  SRR19973065.2096209/1‑150 (MQ=60)

                                                                                                                                   CGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATC             >  SRR19973065.2096179/1‑150 (MQ=60)

                                                                                                                                   CGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATC             >  SRR19973065.2095739/1‑150 (MQ=60)

                                                                                                                                   CGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCGGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATC             <  SRR19973065.2822443/150‑1 (MQ=60)

                                                                                                                                   CGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCGGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATC             <  SRR19973065.2822849/150‑1 (MQ=60)

                                                                                                                                    GTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCC            <  SRR19973065.3209182/150‑1 (MQ=60)

                                                                                                                                     TCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCT           >  SRR19973065.1596136/1‑150 (MQ=60)

                                                                                                                                      CATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTG          <  SRR19973065.2790468/150‑1 (MQ=60)

                                                                                                                                          TACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCT      <  SRR19973065.1509837/150‑1 (MQ=60)

                                                                                                                                          TACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCT      <  SRR19973065.3261216/150‑1 (MQ=60)

                                                                                                                                          TACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCT      >  SRR19973065.969447/1‑150 (MQ=60)

                                                                                                                                              TGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTG  <  SRR19973065.1086721/150‑1 (MQ=60)

CATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTG  >  CP009273/3074967‑3075258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`