Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,075,114 C→A 100% intergenic (‑120/‑158) tktA ← / → loiP transketolase 1, thiamine triphosphate‑binding/Phe‑Phe periplasmic metalloprotease, OM lipoprotein; low salt‑inducible; heat shock protein that binds Era

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,075,1140CA97.8% 865.2 / 6.9 253intergenic (‑120/‑158)tktA/loiPtransketolase 1, thiamine triphosphate‑binding/Phe‑Phe periplasmic metalloprotease, OM lipoprotein; low salt‑inducible; heat shock protein that binds Era
Reads supporting (aligned to +/- strand):  ref base C (4/2);  new base A (115/131);  total (120/133)
Fisher's exact test for biased strand distribution p-value = 4.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 253 total aligned reads displayed.

ATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTGCG  >  CP009273/3074968‑3075260
                                                                                                                                                  |                                                                                                                                                  
 ttGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATcc                                                                                                                                                >  2:2862019/1‑150 (MQ=255)
  tGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCt                                                                                                                                               <  2:780890/150‑1 (MQ=255)
    gCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCttt                                                                                                                                             <  2:3599764/150‑1 (MQ=255)
     cAAGCTCTTTACGTGAGGACTTTTTGTCTCCAGATAGGATGATGAATGGCACTCCCTTAACTACTTGAAGACATCGCGTTTTGGTCTACGCCGGAAAATTTGCCAACAATTTACCGCATGCAGAGCGTAATGTACTTGGAAAATCCtttt                                                                                                                                            <  2:4023189/150‑1 (MQ=255)
     cAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCtttt                                                                                                                                            >  1:2830657/1‑150 (MQ=255)
     cAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCtttt                                                                                                                                            <  1:1801388/150‑1 (MQ=255)
     cAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCtttt                                                                                                                                            <  1:2620559/150‑1 (MQ=255)
       aGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGc                                                                                                                                          >  1:3943590/1‑150 (MQ=255)
       aGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGc                                                                                                                                          >  1:2804580/1‑150 (MQ=255)
          tctTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGc                                                                                                                                       >  2:143835/1‑150 (MQ=255)
            tttACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGNGCGTCATGTACATGGAAAATCCTTTTGCCGCtt                                                                                                                                     >  1:429276/1‑150 (MQ=255)
            tttACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCtt                                                                                                                                     >  1:3566071/1‑150 (MQ=255)
              tACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCa                                                                                                                                   <  1:2733433/150‑1 (MQ=255)
                  tGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGaaa                                                                                                                               >  2:312282/1‑150 (MQ=255)
                    aGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAAtc                                                                                                                             >  2:3200734/1‑150 (MQ=255)
                    aGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAAtc                                                                                                                             <  1:1240735/150‑1 (MQ=255)
                    aGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAAtc                                                                                                                             <  2:1269248/150‑1 (MQ=255)
                    aGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAAtc                                                                                                                             <  1:1687042/150‑1 (MQ=255)
                        cATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTg                                                                                                                         <  1:1032185/150‑1 (MQ=255)
                        cATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTg                                                                                                                         >  2:1963384/1‑150 (MQ=255)
                         aTTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTgg                                                                                                                        <  2:3378839/150‑1 (MQ=255)
                             tGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACGTGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCa                                                                                                                    <  2:3294068/150‑1 (MQ=255)
                              gACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCAt                                                                                                                   <  2:2741755/150‑1 (MQ=255)
                               aCTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATg                                                                                                                  >  2:300481/1‑150 (MQ=255)
                               aCTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATg                                                                                                                  <  2:2033008/150‑1 (MQ=255)
                               aCTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATg                                                                                                                  >  2:2182688/1‑150 (MQ=255)
                               aCTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATg                                                                                                                  >  2:2182985/1‑150 (MQ=255)
                                    aGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGc                                                                                                             >  1:2030057/1‑150 (MQ=255)
                                     gATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCa                                                                                                            <  2:1077544/150‑1 (MQ=255)
                                      aTCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCAt                                                                                                           >  1:1419521/1‑150 (MQ=255)
                                         ggATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGtt                                                                                                        <  2:1889968/150‑1 (MQ=255)
                                         ggATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGtt                                                                                                        >  1:2765925/1‑150 (MQ=255)
                                          gatgatGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTg                                                                                                       <  2:2915930/150‑1 (MQ=255)
                                          gatgatGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTg                                                                                                       <  2:3904535/150‑1 (MQ=255)
                                           atgatgAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTgc                                                                                                      >  1:2960196/1‑150 (MQ=255)
                                           atgatgAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTgc                                                                                                      >  1:609192/1‑150 (MQ=255)
                                             gatgaAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTgcgc                                                                                                    >  1:509738/1‑150 (MQ=255)
                                              atgaAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCa                                                                                                   >  1:2524223/1‑150 (MQ=255)
                                                gaAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAAt                                                                                                 <  2:1490469/150‑1 (MQ=255)
                                                gaAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAAt                                                                                                 >  2:88808/1‑150 (MQ=255)
                                                   gggCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTa                                                                                              <  1:3829898/150‑1 (MQ=255)
                                                      cACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTc                                                                                           <  2:2838929/150‑1 (MQ=255)
                                                       aCGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCg                                                                                          >  1:3940843/1‑150 (MQ=255)
                                                         gCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGcc                                                                                        >  1:2362686/1‑150 (MQ=255)
                                                            cTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGt                                                                                     >  2:536335/1‑150 (MQ=255)
                                                              tAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCACAATCTACTCGCCCGTcc                                                                                   <  2:3065794/150‑1 (MQ=255)
                                                                 cGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCt                                                                                <  1:2839955/150‑1 (MQ=255)
                                                                  gACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTg                                                                               >  1:2667085/1‑150 (MQ=255)
                                                                    cTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTgcg                                                                             >  2:3430990/1‑150 (MQ=255)
                                                                        acgacAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCttt                                                                         <  1:672729/150‑1 (MQ=255)
                                                                             aGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCtt                                                                    <  2:2477189/150‑1 (MQ=255)
                                                                             aGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCtt                                                                    <  2:373518/150‑1 (MQ=255)
                                                                                  gTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATact                                                               >  1:1524541/1‑150 (MQ=255)
                                                                                  gTTTTGGGCTACGCCGGAAAATTTGCCAACAAATAACCGCAAGCCGCGCGTCATGTACAAGGAAAATCCTTTTGCAGCGTCAGAAAACTCAGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATact                                                               >  2:2654452/1‑150 (MQ=255)
                                                                                   ttttGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATactg                                                              <  1:4157075/150‑1 (MQ=255)
                                                                                   ttttGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATactg                                                              <  1:1300951/150‑1 (MQ=255)
                                                                                     ttGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATactgag                                                            <  1:216814/150‑1 (MQ=255)
                                                                                      tGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATactgaga                                                           >  2:3681934/1‑150 (MQ=255)
                                                                                        ggCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATactgagact                                                         >  1:4041927/1‑150 (MQ=255)
                                                                                          cTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATactgagactga                                                       <  1:3531102/150‑1 (MQ=255)
                                                                                           tACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCAGGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATactgagactgag                                                      >  2:1759956/1‑150 (MQ=255)
                                                                                              gCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGt                                                   >  2:2307809/1‑150 (MQ=255)
                                                                                               ccGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTc                                                  <  2:767470/150‑1 (MQ=255)
                                                                                                cGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCg                                                 >  2:2984946/1‑150 (MQ=255)
                                                                                                 ggAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGa                                                >  2:2549430/1‑150 (MQ=255)
                                                                                                 ggAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGa                                                >  2:376504/1‑150 (MQ=255)
                                                                                                 ggAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGa                                                <  1:150264/150‑1 (MQ=255)
                                                                                                    aaaTTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTc                                             <  2:2377009/150‑1 (MQ=255)
                                                                                                    aaaTTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTc                                             >  2:3269573/1‑150 (MQ=255)
                                                                                                     aaTTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTcc                                            <  1:3643122/150‑2 (MQ=255)
                                                                                                     aaTTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCa                                            <  2:682595/150‑1 (MQ=255)
                                                                                                        ttGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCt                                         <  1:2764854/150‑1 (MQ=255)
                                                                                                        ttGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCt                                         <  1:1466740/150‑1 (MQ=255)
                                                                                                         tGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTg                                        >  2:4152033/1‑150 (MQ=255)
                                                                                                          gCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGc                                       <  1:3748881/150‑1 (MQ=255)
                                                                                                            caacaaTTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCaa                                     <  2:2801108/150‑1 (MQ=255)
                                                                                                            caacaaTTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCaa                                     >  2:664841/1‑150 (MQ=255)
                                                                                                             aacaaTTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCaaa                                    >  1:514522/1‑150 (MQ=255)
                                                                                                                   ttACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGgcgc                              <  1:4062351/150‑1 (MQ=255)
                                                                                                                    tACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCa                             <  2:3569963/150‑1 (MQ=255)
                                                                                                                    tACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCa                             >  1:3317711/1‑150 (MQ=255)
                                                                                                                    tACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCa                             >  1:2649705/1‑150 (MQ=255)
                                                                                                                     aCCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCAt                            <  2:1501879/150‑1 (MQ=255)
                                                                                                                     aCCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCAt                            <  2:4005117/150‑1 (MQ=255)
                                                                                                                        gCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCAtttt                         >  1:1099626/1‑150 (MQ=255)
                                                                                                                         cAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCAttttt                        <  2:1559797/150‑1 (MQ=255)
                                                                                                                          aaGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTa                       >  1:2347121/1‑150 (MQ=255)
                                                                                                                          aaGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTa                       >  1:811100/1‑150 (MQ=255)
                                                                                                                            gCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGa                     <  2:511882/150‑1 (MQ=255)
                                                                                                                             ccGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGaa                    >  1:1940719/1‑150 (MQ=255)
                                                                                                                               gcgcgTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGaata                  <  1:311891/150‑1 (MQ=255)
                                                                                                                               gcgcgTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGaata                  <  2:206249/150‑1 (MQ=255)
                                                                                                                                 gcgTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGaataat                <  1:2318108/150‑1 (MQ=255)
                                                                                                                                   gTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATcc              >  2:4111484/1‑150 (MQ=255)
                                                                                                                                   gTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATcc              >  1:743991/1‑150 (MQ=255)
                                                                                                                                       tGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGAc          >  2:1018551/1‑150 (MQ=255)
                                                                                                                                         tACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCt        >  1:3427871/1‑150 (MQ=255)
                                                                                                                                               gAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTTTACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTGCg  <  2:17730/150‑1 (MQ=255)
                                                                                                                                               gAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTGCg  <  1:3208100/150‑1 (MQ=255)
                                                                                                                                               gAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTGCg  <  2:3573471/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
ATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTGCG  >  CP009273/3074968‑3075260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTGCGGA  >  CP009273/3074965‑3075262
                                                                                                                                                     |                                                                                                                                                    
AGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAA                                                                                                                                                      >  SRR19973074.332059/1‑150 (MQ=60)
 GCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAA                                                                                                                                                     <  SRR19973074.1917240/150‑1 (MQ=60)
    TTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCC                                                                                                                                                  <  SRR19973074.2610377/150‑1 (MQ=60)
        CAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTT                                                                                                                                              <  SRR19973074.1804812/150‑1 (MQ=60)
        CAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTT                                                                                                                                              >  SRR19973074.2835894/1‑150 (MQ=60)
        CAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTT                                                                                                                                              >  SRR19973074.3072186/1‑150 (MQ=60)
        CAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTT                                                                                                                                              <  SRR19973074.2625459/150‑1 (MQ=60)
          AGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGC                                                                                                                                            >  SRR19973074.3950767/1‑150 (MQ=60)
          AGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGC                                                                                                                                            >  SRR19973074.2809757/1‑150 (MQ=60)
          AGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGC                                                                                                                                            >  SRR19973074.2809744/1‑150 (MQ=60)
          AGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGC                                                                                                                                            >  SRR19973074.3950857/1‑150 (MQ=60)
              CTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCT                                                                                                                                        <  SRR19973074.397002/150‑1 (MQ=60)
              CTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCT                                                                                                                                        <  SRR19973074.397010/150‑1 (MQ=60)
              CTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCT                                                                                                                                        <  SRR19973074.2836117/150‑1 (MQ=60)
               TTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGNGCGTCATGTACATGGAAAATCCTTTTGCCGCTT                                                                                                                                       >  SRR19973074.430101/1‑150 (MQ=60)
               TTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTT                                                                                                                                       <  SRR19973074.4119318/150‑1 (MQ=60)
               TTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTT                                                                                                                                       >  SRR19973074.3572649/1‑150 (MQ=60)
                 TACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCA                                                                                                                                     <  SRR19973074.2738479/150‑1 (MQ=60)
                   CGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGA                                                                                                                                   >  SRR19973074.3737222/1‑150 (MQ=60)
                      GAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAAT                                                                                                                                >  SRR19973074.3528681/1‑150 (MQ=60)
                      GAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAAT                                                                                                                                >  SRR19973074.3528720/1‑150 (MQ=60)
                       AGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATC                                                                                                                               <  SRR19973074.1690198/150‑1 (MQ=60)
                       AGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATC                                                                                                                               <  SRR19973074.1243113/150‑1 (MQ=60)
                           CATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTG                                                                                                                           >  SRR19973074.545614/1‑150 (MQ=60)
                           CATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTG                                                                                                                           <  SRR19973074.1034200/150‑1 (MQ=60)
                           CATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTG                                                                                                                           <  SRR19973074.1034088/150‑1 (MQ=60)
                            ATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGG                                                                                                                          >  SRR19973074.1489470/1‑150 (MQ=60)
                              TTTTACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGAT                                                                                                                        <  SRR19973074.1116078/150‑1 (MQ=60)
                              TTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGAT                                                                                                                        <  SRR19973074.2695498/150‑1 (MQ=60)
                                    TCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCT                                                                                                                  <  SRR19973074.1348838/150‑1 (MQ=60)
                                    TCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCT                                                                                                                  <  SRR19973074.1348784/150‑1 (MQ=60)
                                     CCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTC                                                                                                                 <  SRR19973074.393276/150‑1 (MQ=60)
                                     CCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTC                                                                                                                 <  SRR19973074.2935762/150‑1 (MQ=60)
                                       AGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAGATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGC                                                                                                               >  SRR19973074.1554528/1‑150 (MQ=60)
                                       AGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGC                                                                                                               >  SRR19973074.2033896/1‑150 (MQ=60)
                                       AGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGC                                                                                                               >  SRR19973074.1910731/1‑150 (MQ=60)
                                         ATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCAT                                                                                                             >  SRR19973074.2121278/1‑150 (MQ=60)
                                         ATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCAT                                                                                                             >  SRR19973074.2121463/1‑150 (MQ=60)
                                         ATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCAT                                                                                                             >  SRR19973074.1422148/1‑150 (MQ=60)
                                            GGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTT                                                                                                          >  SRR19973074.2770962/1‑150 (MQ=60)
                                            GGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTT                                                                                                          >  SRR19973074.4179416/1‑150 (MQ=60)
                                            GGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTT                                                                                                          >  SRR19973074.2771011/1‑150 (MQ=60)
                                              ATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGC                                                                                                        >  SRR19973074.610348/1‑150 (MQ=60)
                                              ATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGC                                                                                                        >  SRR19973074.2965698/1‑150 (MQ=60)
                                                GATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGC                                                                                                      >  SRR19973074.512905/1‑150 (MQ=60)
                                                GATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGC                                                                                                      >  SRR19973074.510756/1‑150 (MQ=60)
                                                 ATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCA                                                                                                     >  SRR19973074.2528930/1‑150 (MQ=60)
                                                 ATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCA                                                                                                     >  SRR19973074.2529156/1‑150 (MQ=60)
                                                 ATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCA                                                                                                     >  SRR19973074.4080536/1‑150 (MQ=60)
                                                     AGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCT                                                                                                 <  SRR19973074.2287647/150‑1 (MQ=60)
                                                     AGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATACTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCT                                                                                                 <  SRR19973074.880040/150‑1 (MQ=60)
                                                      GGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTA                                                                                                <  SRR19973074.3836880/150‑1 (MQ=60)
                                                          ACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCG                                                                                            >  SRR19973074.3948102/1‑150 (MQ=60)
                                                            GCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCC                                                                                          >  SRR19973074.2367084/1‑150 (MQ=60)
                                                            GCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCC                                                                                          >  SRR19973074.2367059/1‑150 (MQ=60)
                                                              CCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCG                                                                                        <  SRR19973074.1520185/150‑1 (MQ=60)
                                                              CCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCG                                                                                        <  SRR19973074.504178/150‑1 (MQ=60)
                                                                TTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTC                                                                                      <  SRR19973074.2667784/150‑1 (MQ=60)
                                                                TTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTC                                                                                      <  SRR19973074.2668024/150‑1 (MQ=60)
                                                                    CGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCT                                                                                  <  SRR19973074.2845214/150‑1 (MQ=60)
                                                                    CGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCT                                                                                  <  SRR19973074.2845167/150‑1 (MQ=60)
                                                                     GACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTG                                                                                 >  SRR19973074.2672054/1‑150 (MQ=60)
                                                                      ACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGC                                                                                >  SRR19973074.599160/1‑150 (MQ=60)
                                                                         TGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCT                                                                             >  SRR19973074.2489286/1‑150 (MQ=60)
                                                                           ACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTT                                                                           <  SRR19973074.3622908/150‑1 (MQ=60)
                                                                           ACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTT                                                                           <  SRR19973074.673968/150‑1 (MQ=60)
                                                                           ACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTT                                                                           <  SRR19973074.2503851/150‑1 (MQ=60)
                                                                             GACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTC                                                                         <  SRR19973074.3233090/150‑1 (MQ=60)
                                                                                     GTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACT                                                                 >  SRR19973074.1527420/1‑150 (MQ=60)
                                                                                      TTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTG                                                                <  SRR19973074.1303388/150‑1 (MQ=60)
                                                                                      TTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTG                                                                <  SRR19973074.1303410/150‑1 (MQ=60)
                                                                                      TTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTG                                                                <  SRR19973074.4164725/150‑1 (MQ=60)
                                                                                        TTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAG                                                              <  SRR19973074.217261/150‑1 (MQ=60)
                                                                                        TTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAG                                                              <  SRR19973074.701194/150‑1 (MQ=60)
                                                                                        TTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAG                                                              <  SRR19973074.377850/150‑1 (MQ=60)
                                                                                           GGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACT                                                           >  SRR19973074.2641866/1‑150 (MQ=60)
                                                                                           GGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACT                                                           >  SRR19973074.4049398/1‑150 (MQ=60)
                                                                                             CTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGA                                                         <  SRR19973074.3537606/150‑1 (MQ=60)
                                                                                             CTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGATCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGA                                                         >  SRR19973074.2794296/1‑150 (MQ=60)
                                                                                              TACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAG                                                        <  SRR19973074.1939756/150‑1 (MQ=60)
                                                                                                    GGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGA                                                  <  SRR19973074.150605/150‑1 (MQ=60)
                                                                                                     GAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGAT                                                 <  SRR19973074.680254/150‑1 (MQ=60)
                                                                                                       AAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTC                                               >  SRR19973074.3539331/1‑150 (MQ=60)
                                                                                                       AAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTC                                               >  SRR19973074.3678728/1‑150 (MQ=60)
                                                                                                        AATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCC                                              <  SRR19973074.3649850/150‑1 (MQ=60)
                                                                                                        AATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCC                                              <  SRR19973074.3649855/150‑1 (MQ=60)
                                                                                                          TTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACC                                            <  SRR19973074.1625802/150‑1 (MQ=60)
                                                                                                          TTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACC                                            >  SRR19973074.473753/1‑150 (MQ=60)
                                                                                                           TTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCT                                           <  SRR19973074.2769881/150‑1 (MQ=60)
                                                                                                           TTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCT                                           <  SRR19973074.1469493/150‑1 (MQ=60)
                                                                                                           TTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCT                                           <  SRR19973074.2769938/150‑1 (MQ=60)
                                                                                                             GCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGC                                         <  SRR19973074.3755775/150‑1 (MQ=60)
                                                                                                                AACAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAA                                      >  SRR19973074.515549/1‑150 (MQ=60)
                                                                                                                  CAATTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACG                                    <  SRR19973074.1429431/150‑1 (MQ=60)
                                                                                                                     TTTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCG                                 >  SRR19973074.333844/1‑150 (MQ=60)
                                                                                                                      TTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGC                                <  SRR19973074.4069863/150‑1 (MQ=60)
                                                                                                                      TTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGC                                <  SRR19973074.1966988/150‑1 (MQ=60)
                                                                                                                      TTACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGC                                <  SRR19973074.4069862/150‑1 (MQ=60)
                                                                                                                       TACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCA                               >  SRR19973074.3323868/1‑150 (MQ=60)
                                                                                                                       TACCGCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCA                               >  SRR19973074.2654647/1‑150 (MQ=60)
                                                                                                                           GCAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTT                           >  SRR19973074.1101687/1‑150 (MQ=60)
                                                                                                                            CAAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTT                          <  SRR19973074.891287/150‑1 (MQ=60)
                                                                                                                             AAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTA                         <  SRR19973074.3312987/150‑1 (MQ=60)
                                                                                                                             AAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTA                         >  SRR19973074.812662/1‑150 (MQ=60)
                                                                                                                             AAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTA                         >  SRR19973074.2351504/1‑150 (MQ=60)
                                                                                                                             AAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTA                         >  SRR19973074.2351490/1‑150 (MQ=60)
                                                                                                                             AAGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTA                         >  SRR19973074.812637/1‑150 (MQ=60)
                                                                                                                              AGCCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAG                        >  SRR19973074.2809940/1‑150 (MQ=60)
                                                                                                                                CCGCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAA                      >  SRR19973074.1944429/1‑150 (MQ=60)
                                                                                                                                  GCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATA                    <  SRR19973074.1226902/150‑1 (MQ=60)
                                                                                                                                  GCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATA                    <  SRR19973074.312464/150‑1 (MQ=60)
                                                                                                                                  GCGCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATA                    <  SRR19973074.312453/150‑1 (MQ=60)
                                                                                                                                    GCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAAT                  <  SRR19973074.2322718/150‑1 (MQ=60)
                                                                                                                                    GCGTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAAT                  <  SRR19973074.2322441/150‑1 (MQ=60)
                                                                                                                                      GTCATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCC                >  SRR19973074.745394/1‑150 (MQ=60)
                                                                                                                                        CATGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTG              >  SRR19973074.2759532/1‑150 (MQ=60)
                                                                                                                                          TGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGAC            <  SRR19973074.3849284/150‑1 (MQ=60)
                                                                                                                                          TGTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGAC            <  SRR19973074.1747122/150‑1 (MQ=60)
                                                                                                                                           GTACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACC           >  SRR19973074.1471726/1‑150 (MQ=60)
                                                                                                                                            TACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCT          >  SRR19973074.3434192/1‑150 (MQ=60)
                                                                                                                                            TACATGGAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCT          >  SRR19973074.627775/1‑150 (MQ=60)
                                                                                                                                                  GAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTGCG    <  SRR19973074.3213982/150‑1 (MQ=60)
                                                                                                                                                  GAAAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTGCG    <  SRR19973074.3214013/150‑1 (MQ=60)
                                                                                                                                                    AAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTGCGGA  >  SRR19973074.2073478/1‑150 (MQ=60)
                                                                                                                                                    AAATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTGCGGA  >  SRR19973074.1181073/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
AGCATTGGCAAGCTCTTTACGTGAGGACATTTTGACTCCAGATCGGATGATGAAGGGCACGCCCTTAACGACTTGACGACAGCGCGTTTTGGGCTACGCCGGAAAATTTGCCAACAATTTACCGCAAGCCGCGCGTCATGTACATGGAACATCCTTTTGCCGCTTCAGAAATCTCTGGATCATGCTCGCATGTTGCGCAATCTACTCGCCCGTCCGCTGCGCTTTTCCTTATACTGAGACTGAGCGTCGATTCACCTGCAAACGGCGCATTTTTAGAATAATCCTGACCTTGTGCGGA  >  CP009273/3074965‑3075262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑