Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F121 I0 R1	856	273.6	9081716	94.1%	8545894	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,097,822	G→T	89.2%	E115* (GAA→TAA)	pfkA →	6‑phosphofructokinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,097,822	0	G	T	89.2%	805.6 / 85.8	282	E115* (GAA→TAA)	pfkA	6‑phosphofructokinase I
Reads supporting (aligned to +/- strand): ref base G (10/21); new base T (122/129); total (132/150)
Fisher's exact test for biased strand distribution p-value = 9.02e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 284 total aligned reads displayed.

GCGGTACGTTCCTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCGAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCTCTTCTTCTCACCAGCG  >  CP009273/4097673‑4097967

gCGGTACGTTCCTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCg                                                                                                                                                   <  2:791875/150‑1 (MQ=255)

   gTACGTTCCTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTaaa                                                                                                                                                >  2:1700898/1‑150 (MQ=255)

   gTACGTTCCTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTaaa                                                                                                                                                >  2:2403565/1‑150 (MQ=255)

     aCGTTCCTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATg                                                                                                                                              <  2:880409/150‑1 (MQ=255)

     aCGTTCCTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATg                                                                                                                                              >  1:339699/1‑150 (MQ=255)

     aCGTTCCTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATg                                                                                                                                              >  1:2546166/1‑150 (MQ=255)

     aCGTTCCTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATg                                                                                                                                              <  2:3345538/150‑1 (MQ=255)

      cGTTCCTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTCTCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATgg                                                                                                                                             >  2:1100090/1‑150 (MQ=255)

       ggtCCTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATggg                                                                                                                                            <  1:3641842/148‑1 (MQ=255)

           cTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTc                                                                                                                                        >  2:1827358/1‑150 (MQ=255)

           cTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTc                                                                                                                                        >  1:4318073/1‑150 (MQ=255)

           cTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTc                                                                                                                                        <  1:546324/150‑1 (MQ=255)

             cGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTccc                                                                                                                                      <  1:849747/150‑1 (MQ=255)

              ggTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCg                                                                                                                                     >  1:2761431/1‑150 (MQ=255)

              ggTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCg                                                                                                                                     >  1:3626190/1‑150 (MQ=255)

                    gcgcGTGTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATc                                                                                                                               <  2:3242392/150‑1 (MQ=255)

                      gcgTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCgg                                                                                                                             >  2:2159820/1‑150 (MQ=255)

                       cgTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGt                                                                                                                            >  2:228661/1‑150 (MQ=255)

                        gTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTc                                                                                                                           >  1:66450/1‑150 (MQ=255)

                        gTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTc                                                                                                                           >  1:954387/1‑150 (MQ=255)

                         tttCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCt                                                                                                                          <  1:4149933/150‑1 (MQ=255)

                          ttCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTg                                                                                                                         <  2:1232429/150‑1 (MQ=255)

                          ttCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTg                                                                                                                         <  2:2908508/150‑1 (MQ=255)

                            cccGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGcc                                                                                                                       <  2:2180824/150‑1 (MQ=255)

                              cGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCGAAATGGGCTTCCCGTGCATCGGTCTGCCgg                                                                                                                     >  1:1841400/1‑150 (MQ=255)

                                  aTTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCAc                                                                                                                 <  2:4345698/150‑1 (MQ=255)

                                   ttCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCGAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACt                                                                                                                <  1:1090443/150‑1 (MQ=255)

                                   ttCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCGAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACt                                                                                                                <  1:1090020/150‑1 (MQ=255)

                                     ccGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTAt                                                                                                              <  2:723145/150‑1 (MQ=255)

                                      cgcgACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATc                                                                                                             <  2:305931/150‑1 (MQ=255)

                                         gacgaGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGAc                                                                                                          >  1:32364/1‑150 (MQ=255)

                                         gacgaGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGAc                                                                                                          >  1:22609/1‑150 (MQ=255)

                                            gagaACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAAc                                                                                                       <  1:3885071/150‑1 (MQ=255)

                                            gagaACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAAc                                                                                                       <  1:2832192/150‑1 (MQ=255)

                                                 cATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACAt                                                                                                  <  1:1701696/150‑1 (MQ=255)

                                                 cATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACAt                                                                                                  >  1:724620/1‑150 (MQ=255)

                                                  aTCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATc                                                                                                 >  1:422067/1‑150 (MQ=255)

                                                   tCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCa                                                                                                >  2:1968810/1‑150 (MQ=255)

                                                   tCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCa                                                                                                <  2:2232758/150‑1 (MQ=255)

                                                   tCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCGGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCa                                                                                                <  2:3194333/150‑1 (MQ=255)

                                                         ccGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCa                                                                                          >  2:2249541/1‑150 (MQ=255)

                                                           gTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACt                                                                                        >  1:3183722/1‑150 (MQ=255)

                                                             ggCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGa                                                                                      <  1:837816/150‑1 (MQ=255)

                                                                tATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTa                                                                                   <  1:2893836/150‑1 (MQ=255)

                                                                tATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTa                                                                                   <  1:2319890/150‑1 (MQ=255)

                                                                tATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTa                                                                                   <  1:3689072/150‑1 (MQ=255)

                                                                   cGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTacac                                                                                <  1:3243952/150‑1 (MQ=255)

                                                                   cGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCGAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTacac                                                                                >  2:2479285/1‑150 (MQ=255)

                                                                    gAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACt                                                                               <  1:2629728/150‑1 (MQ=255)

                                                                     aaaaCCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCGAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTa                                                                              >  1:3733942/1‑150 (MQ=255)

                                                                     aaaaCCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCGAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTa                                                                              >  2:1341992/1‑150 (MQ=255)

                                                                      aaaCCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTAt                                                                             >  2:2974019/1‑150 (MQ=255)

                                                                        aCCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCGAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCg                                                                           <  2:1658685/150‑1 (MQ=255)

                                                                         ccTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCgg                                                                          >  2:677704/1‑150 (MQ=255)

                                                                         ccTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCgg                                                                          >  2:1939259/1‑150 (MQ=255)

                                                                          cTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGt                                                                         >  2:1456628/1‑150 (MQ=255)

                                                                            gAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCGAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGttt                                                                       <  1:4178544/150‑1 (MQ=255)

                                                                               aaaaCGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTttctt                                                                    <  1:2230875/150‑1 (MQ=255)

                                                                               aaaaCGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTttctt                                                                    >  2:36957/1‑150 (MQ=255)

                                                                               aaaaCGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTttctt                                                                    <  2:2659434/150‑1 (MQ=255)

                                                                               aaaaCGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTttctt                                                                    <  1:4369914/150‑1 (MQ=255)

                                                                                 aaCGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCa                                                                  <  1:2981940/150‑1 (MQ=255)

                                                                                   cGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACt                                                                >  2:929646/1‑150 (MQ=255)

                                                                                   cGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCAACGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCaat                                                                >  2:4439352/1‑148 (MQ=255)

                                                                                    gTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTg                                                               <  2:4215582/150‑1 (MQ=255)

                                                                                      ggTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTgcg                                                             >  2:175793/1‑150 (MQ=255)

                                                                                        tATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCt                                                           >  1:1432937/1‑150 (MQ=255)

                                                                                        tATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCt                                                           >  2:3590189/1‑150 (MQ=255)

                                                                                        tATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTCCACTGCGCt                                                           <  2:2242797/150‑1 (MQ=255)

                                                                                        tATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCGAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCt                                                           <  2:1729763/150‑1 (MQ=255)

                                                                                         aTCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTg                                                          >  1:393760/1‑150 (MQ=255)

                                                                                          tCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGa                                                         >  2:1208883/1‑150 (MQ=255)

                                                                                           cGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAg                                                        <  2:2147834/150‑1 (MQ=255)

                                                                                           cGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAg                                                        >  2:527648/1‑150 (MQ=255)

                                                                                           cGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAg                                                        <  1:2375932/150‑1 (MQ=255)

                                                                                            gACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGc                                                       >  2:1457104/1‑150 (MQ=255)

                                                                                              cgcgCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCAc                                                     >  1:3738862/1‑150 (MQ=255)

                                                                                              cgcgCTGGAGGTTATCGGCGGTGACGGTTCCTACATGCGAGCAAAGCGTCTGACCTAAATGGGCTTCCCGTGCAACGGTCTGCCGGGCACTAACGACAACGACATCAAAGGCACTGACTACACTATCGGATTCTTCACTGCGCTGAGCAc                                                     >  2:3397259/1‑150 (MQ=255)

                                                                                                cgcgGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCg                                                   >  1:510662/1‑150 (MQ=255)

                                                                                                cgcTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCg                                                   >  1:3364375/1‑150 (MQ=255)

                                                                                                  cTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGtt                                                 <  2:552751/150‑1 (MQ=255)

                                                                                                   tggtggTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTg                                                >  2:3036031/1‑150 (MQ=255)

                                                                                                    ggtggtTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGt                                               <  2:2927709/150‑1 (MQ=255)

                                                                                                    ggtggtTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGt                                               >  1:3283033/1‑150 (MQ=255)

                                                                                                              ggcggTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGACGCGATc                                     >  2:2499079/1‑150 (MQ=255)

                                                                                                                 ggTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGAc                                  <  2:4128680/150‑1 (MQ=255)

                                                                                                                    gACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGt                               >  1:3983881/1‑150 (MQ=255)

                                                                                                                      cGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCt                             <  2:1706457/150‑1 (MQ=255)

                                                                                                                      cGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCt                             >  1:2103967/1‑150 (MQ=255)

                                                                                                                         ttCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGAAAACGACATAAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCg                          <  1:4399211/150‑1 (MQ=255)

                                                                                                                             tACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGac                      >  2:2855289/1‑150 (MQ=255)

                                                                                                                              aCATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGaca                     <  1:3487974/150‑1 (MQ=255)

                                                                                                                                    gTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCtctt               <  2:392309/150‑1 (MQ=255)

                                                                                                                                    gTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCtctt               <  2:392485/150‑1 (MQ=255)

                                                                                                                                      gCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCtcttct             <  2:256933/150‑1 (MQ=255)

                                                                                                                                      gCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCtcttct             <  1:3832613/150‑1 (MQ=255)

                                                                                                                                              tCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCTCTTCTTCTCACCa     <  1:1792325/150‑1 (MQ=255)

                                                                                                                                                tGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCTCTTCTTCTCACCAGc   <  2:1907957/150‑1 (MQ=255)

                                                                                                                                                 gACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCTCTTCTTCTCACCAGCg  <  2:1505346/150‑1 (MQ=255)

                                                                                                                                                 gACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCTCTTCTTCTCACCAGCg  <  2:251455/150‑1 (MQ=255)

GCGGTACGTTCCTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCGAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCTCTTCTTCTCACCAGCG  >  CP009273/4097673‑4097967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GTACGTTCCTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCGAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCTCTTCTTCTCACCAGCGTATT  >  CP009273/4097676‑4097971

GTACGTTCCTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAA                                                                                                                                                    >  SRR19973080.1764506/1‑150 (MQ=60)

 TACGTTCCTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAAT                                                                                                                                                   >  SRR19973080.2614027/1‑150 (MQ=60)

  ACGTTCCTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATG                                                                                                                                                  >  SRR19973080.2560135/1‑150 (MQ=60)

  ACGTTCCTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATG                                                                                                                                                  >  SRR19973080.2560111/1‑150 (MQ=60)

  ACGTTCCTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATG                                                                                                                                                  <  SRR19973080.3345473/150‑1 (MQ=60)

  ACGTTCCTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATG                                                                                                                                                  >  SRR19973080.341738/1‑150 (MQ=60)

  ACGTTCCTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATG                                                                                                                                                  <  SRR19973080.1875575/150‑1 (MQ=60)

   CGTTCCTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGG                                                                                                                                                 >  SRR19973080.3705881/1‑150 (MQ=60)

    GTTCCTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGG                                                                                                                                                <  SRR19973080.4559475/150‑1 (MQ=60)

    GGTCCTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGG                                                                                                                                                <  SRR19973080.3661949/150‑1 (MQ=60)

      TCCTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCT                                                                                                                                              >  SRR19973080.581041/1‑150 (MQ=60)

        CTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTC                                                                                                                                            <  SRR19973080.549794/150‑1 (MQ=60)

        CTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTC                                                                                                                                            <  SRR19973080.550678/150‑1 (MQ=60)

        CTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTC                                                                                                                                            >  SRR19973080.4341663/1‑150 (MQ=60)

        CTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCGAAATGGGCTTC                                                                                                                                            <  SRR19973080.280728/150‑1 (MQ=60)

         TCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCC                                                                                                                                           >  SRR19973080.1791921/1‑150 (MQ=60)

          CGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCC                                                                                                                                          <  SRR19973080.93348/150‑1 (MQ=60)

          CGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCC                                                                                                                                          <  SRR19973080.2883693/150‑1 (MQ=60)

          CGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCC                                                                                                                                          <  SRR19973080.854403/150‑1 (MQ=60)

          CGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCC                                                                                                                                          <  SRR19973080.854883/150‑1 (MQ=60)

           GGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCG                                                                                                                                         >  SRR19973080.2776664/1‑150 (MQ=60)

           GGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCG                                                                                                                                         >  SRR19973080.380861/1‑150 (MQ=60)

           GGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCG                                                                                                                                         >  SRR19973080.3646231/1‑150 (MQ=60)

                 GCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCGAAATGGGCTTCCCGTGCATC                                                                                                                                   <  SRR19973080.375451/150‑1 (MQ=60)

                     GTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTC                                                                                                                               >  SRR19973080.2528966/1‑150 (MQ=60)

                     GTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTC                                                                                                                               >  SRR19973080.67102/1‑150 (MQ=60)

                     GTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTC                                                                                                                               >  SRR19973080.960189/1‑150 (MQ=60)

                     GTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCGAAATGGGCTTCCCGTGCATCGGTC                                                                                                                               >  SRR19973080.684586/1‑150 (MQ=60)

                      TTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCT                                                                                                                              <  SRR19973080.525403/150‑1 (MQ=60)

                      TTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCT                                                                                                                              <  SRR19973080.3278217/150‑1 (MQ=60)

                      TTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCT                                                                                                                              <  SRR19973080.4172351/150‑1 (MQ=60)

                      TTTCCCGGAAGTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTTACCTAAATGGGCTTCCCGTGCATCGGTCT                                                                                                                              <  SRR19973080.3278176/150‑1 (MQ=60)

                           CGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGG                                                                                                                         >  SRR19973080.2958324/1‑150 (MQ=60)

                           CGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCGAAATGGGCTTCCCGTGCATCGGTCTGCCGG                                                                                                                         >  SRR19973080.1851766/1‑150 (MQ=60)

                              AATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCA                                                                                                                      >  SRR19973080.3247449/1‑150 (MQ=60)

                              AATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCA                                                                                                                      >  SRR19973080.3247656/1‑150 (MQ=60)

                              AATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCA                                                                                                                      <  SRR19973080.1807893/150‑1 (MQ=60)

                                TTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCGAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACT                                                                                                                    <  SRR19973080.1096733/150‑1 (MQ=60)

                                TTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCGAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACT                                                                                                                    <  SRR19973080.1096742/150‑1 (MQ=60)

                                TTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCGAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACT                                                                                                                    <  SRR19973080.1096318/150‑1 (MQ=60)

                                  CCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTAT                                                                                                                  >  SRR19973080.4354670/1‑150 (MQ=60)

                                      GACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGAC                                                                                                              >  SRR19973080.32712/1‑150 (MQ=60)

                                      GACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGAC                                                                                                              >  SRR19973080.3648554/1‑150 (MQ=60)

                                      GACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGAC                                                                                                              >  SRR19973080.22865/1‑150 (MQ=60)

                                      GACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGAC                                                                                                              >  SRR19973080.22877/1‑150 (MQ=60)

                                         GAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAAC                                                                                                           <  SRR19973080.2847762/150‑1 (MQ=60)

                                         GAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAAC                                                                                                           <  SRR19973080.3906481/150‑1 (MQ=60)

                                            AACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGAC                                                                                                        >  SRR19973080.3162907/1‑150 (MQ=60)

                                            AACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGAC                                                                                                        >  SRR19973080.3163129/1‑150 (MQ=60)

                                            AACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGAC                                                                                                        >  SRR19973080.2842411/1‑150 (MQ=60)

                                              CATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACAT                                                                                                      <  SRR19973080.1711588/150‑1 (MQ=60)

                                              CATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACAT                                                                                                      >  SRR19973080.728780/1‑150 (MQ=60)

                                               ATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATC                                                                                                     >  SRR19973080.424650/1‑150 (MQ=60)

                                                TCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCA                                                                                                    <  SRR19973080.2269426/150‑1 (MQ=60)

                                                  CGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAA                                                                                                  <  SRR19973080.2855078/150‑1 (MQ=60)

                                                     GCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGC                                                                                               >  SRR19973080.3856667/1‑150 (MQ=60)

                                                        GTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACT                                                                                            >  SRR19973080.1120513/1‑150 (MQ=60)

                                                        GTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACT                                                                                            <  SRR19973080.2020401/150‑1 (MQ=60)

                                                        GTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACT                                                                                            >  SRR19973080.3201296/1‑150 (MQ=60)

                                                          GGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGA                                                                                          <  SRR19973080.842858/150‑1 (MQ=60)

                                                            CTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACT                                                                                        <  SRR19973080.3902657/150‑1 (MQ=60)

                                                             TATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTA                                                                                       <  SRR19973080.2332763/150‑1 (MQ=60)

                                                             TATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTA                                                                                       <  SRR19973080.2909649/150‑1 (MQ=60)

                                                             TATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTA                                                                                       <  SRR19973080.3709356/150‑1 (MQ=60)

                                                             TATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTA                                                                                       >  SRR19973080.734260/1‑150 (MQ=60)

                                                               TCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACA                                                                                     >  SRR19973080.3648653/1‑150 (MQ=60)

                                                                CGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACAC                                                                                    <  SRR19973080.3261811/150‑1 (MQ=60)

                                                                 GAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACT                                                                                   <  SRR19973080.2643958/150‑1 (MQ=60)

                                                                 GAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACT                                                                                   >  SRR19973080.1547537/1‑150 (MQ=60)

                                                                  AAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCGAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTA                                                                                  >  SRR19973080.3754383/1‑150 (MQ=60)

                                                                  AAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCGAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTA                                                                                  >  SRR19973080.3754367/1‑150 (MQ=60)

                                                                       CTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGT                                                                             >  SRR19973080.524121/1‑150 (MQ=60)

                                                                        TGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTT                                                                            <  SRR19973080.671772/150‑1 (MQ=60)

                                                                        TGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCGAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTT                                                                            <  SRR19973080.3813657/150‑1 (MQ=60)

                                                                         GAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCGAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTT                                                                           <  SRR19973080.4201171/150‑1 (MQ=60)

                                                                         GAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCGAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTT                                                                           <  SRR19973080.4201838/150‑1 (MQ=60)

                                                                            AAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTT                                                                        <  SRR19973080.2243159/150‑1 (MQ=60)

                                                                            AAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTT                                                                        <  SRR19973080.3398446/150‑1 (MQ=60)

                                                                            AAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTT                                                                        <  SRR19973080.4393748/150‑1 (MQ=60)

                                                                              AACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCA                                                                      >  SRR19973080.931508/1‑150 (MQ=60)

                                                                              AACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCA                                                                      <  SRR19973080.2998037/150‑1 (MQ=60)

                                                                               ACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCAC                                                                     <  SRR19973080.2258844/150‑1 (MQ=60)

                                                                                CGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACT                                                                    <  SRR19973080.463778/150‑1 (MQ=60)

                                                                                     TATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCT                                                               >  SRR19973080.1441317/1‑150 (MQ=60)

                                                                                     TATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCT                                                               <  SRR19973080.3727076/150‑1 (MQ=60)

                                                                                      ATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTG                                                              >  SRR19973080.396076/1‑150 (MQ=60)

                                                                                       TCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTATATGGGCTTCCCGTGCATCGGTCTGCAGGGCACCATCGACATCGACATCAAAGGCACTGACTACACTATCGGTTTCTTCAGTGCGCTGA                                                             <  SRR19973080.1434465/150‑1 (MQ=60)

                                                                                        CGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAG                                                            <  SRR19973080.2389146/150‑1 (MQ=60)

                                                                                        CGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAG                                                            <  SRR19973080.2388977/150‑1 (MQ=60)

                                                                                           CGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCAC                                                         >  SRR19973080.3756666/1‑150 (MQ=60)

                                                                                           CGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCAC                                                         >  SRR19973080.3759298/1‑150 (MQ=60)

                                                                                           CGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCAC                                                         <  SRR19973080.4239072/150‑1 (MQ=60)

                                                                                             CGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCG                                                       >  SRR19973080.3382622/1‑150 (MQ=60)

                                                                                             CGCGGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCG                                                       >  SRR19973080.513913/1‑150 (MQ=60)

                                                                                                 GGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGT                                                   >  SRR19973080.3301060/1‑150 (MQ=60)

                                                                                                 GGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGT                                                   >  SRR19973080.3301766/1‑150 (MQ=60)

                                                                                                 GGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGT                                                   <  SRR19973080.1686924/150‑1 (MQ=60)

                                                                                                   TGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAG                                                 >  SRR19973080.3939622/1‑150 (MQ=60)

                                                                                                      TTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAG                                              <  SRR19973080.2721390/150‑1 (MQ=60)

                                                                                                          CGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGTGCACTATCGACTACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCTCTGCGCTGAGCACCGTTGTAGAAGCGAT                                          <  SRR19973080.3256572/150‑1 (MQ=60)

                                                                                                           GGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATC                                         <  SRR19973080.2515594/150‑1 (MQ=60)

                                                                                                            GCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCG                                        <  SRR19973080.4022803/150‑1 (MQ=60)

                                                                                                              GGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGAC                                      >  SRR19973080.2956800/1‑150 (MQ=60)

                                                                                                              GGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCGAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGAC                                      <  SRR19973080.4373788/150‑1 (MQ=60)

                                                                                                                 GACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGT                                   >  SRR19973080.3973069/1‑150 (MQ=60)

                                                                                                                 GACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGT                                   >  SRR19973080.4005793/1‑150 (MQ=60)

                                                                                                                   GGGTTCCTACTTGGGTGAAATGCGTCTGACCTAAATGGGCTGCCCGTGCATCGGTCTGCCGGGAACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCT                                 <  SRR19973080.2304994/150‑1 (MQ=60)

                                                                                                                   CGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCT                                 <  SRR19973080.4176595/150‑1 (MQ=60)

                                                                                                                   CGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCT                                 <  SRR19973080.4176587/150‑1 (MQ=60)

                                                                                                                   CGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCT                                 >  SRR19973080.2115849/1‑150 (MQ=60)

                                                                                                                   CGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCT                                 <  SRR19973080.3409094/150‑1 (MQ=60)

                                                                                                                    GGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTG                                >  SRR19973080.352844/1‑150 (MQ=60)

                                                                                                                    GGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTG                                >  SRR19973080.4147422/1‑150 (MQ=60)

                                                                                                                      TTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCG                              <  SRR19973080.4423003/150‑1 (MQ=60)

                                                                                                                      TTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGAAAACGACATAAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCG                              <  SRR19973080.4423161/150‑1 (MQ=60)

                                                                                                                      TTCCTACAGGGGTGCAATGCGTCTGACCGAAAGGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCG                              <  SRR19973080.810803/150‑1 (MQ=60)

                                                                                                                       TCCTACATGGGTGCAATGCGTCTGACCGAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGT                             >  SRR19973080.970903/1‑150 (MQ=60)

                                                                                                                          TACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGAC                          >  SRR19973080.2356520/1‑150 (MQ=60)

                                                                                                                           ACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACA                         <  SRR19973080.3507234/150‑1 (MQ=60)

                                                                                                                           ACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACA                         <  SRR19973080.3507190/150‑1 (MQ=60)

                                                                                                                           ACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACA                         <  SRR19973080.3506655/150‑1 (MQ=60)

                                                                                                                           ACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACA                         >  SRR19973080.1810082/1‑150 (MQ=60)

                                                                                                                                   GCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCTCTTCT                 >  SRR19973080.3942431/1‑150 (MQ=60)

                                                                                                                                   GCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCTCTTCT                 <  SRR19973080.3853656/150‑1 (MQ=60)

                                                                                                                                   GCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCTCTTCT                 <  SRR19973080.3419497/150‑1 (MQ=60)

                                                                                                                                           TCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCTCTTCTTCTCACCA         <  SRR19973080.4290670/150‑1 (MQ=60)

                                                                                                                                           TCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCTCTTCTTCTCACCA         <  SRR19973080.4289716/150‑1 (MQ=60)

                                                                                                                                           TCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCTCTTCTTCTCACCA         <  SRR19973080.1802543/150‑1 (MQ=60)

                                                                                                                                             TGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCTCTTCTTCTCACCAGC       >  SRR19973080.2120932/1‑150 (MQ=60)

                                                                                                                                                  TAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCTCTTCTTCTCACCAGCGTATT  >  SRR19973080.667686/1‑150 (MQ=60)

                                                                                                                                                  TAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCTCTTCTTCTCACCAGCGTATT  >  SRR19973080.2940560/1‑150 (MQ=60)

GTACGTTCCTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCGAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCTCTTCTTCTCACCAGCGTATT  >  CP009273/4097676‑4097971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`