Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,097,822 G→T 100% E115* (GAA→TAA)  pfkA → 6‑phosphofructokinase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,097,8220GT98.8% 883.3 / 1.3 251E115* (GAA→TAA) pfkA6‑phosphofructokinase I
Reads supporting (aligned to +/- strand):  ref base G (3/0);  new base T (134/113);  total (138/113)
Fisher's exact test for biased strand distribution p-value = 2.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 251 total aligned reads displayed.

GGTACGTTCCTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCGAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCTCTTCTTCTCACCAGCGT  >  CP009273/4097675‑4097968
                                                                                                                                                   |                                                                                                                                                  
ggTACGTTCCTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTaa                                                                                                                                                  >  1:4105061/1‑150 (MQ=255)
ggTACGTTCCTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTaa                                                                                                                                                  >  2:427322/1‑150 (MQ=255)
     gTTCCTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATggg                                                                                                                                             >  1:1860609/1‑150 (MQ=255)
     gTTCCTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATggg                                                                                                                                             <  2:3533190/150‑1 (MQ=255)
     gTTCCTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGAGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATggg                                                                                                                                             >  2:2629246/1‑150 (MQ=255)
        ccTCGGTTCTGCTCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCtt                                                                                                                                          >  1:2470790/1‑150 (MQ=255)
        ccTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCtt                                                                                                                                          <  2:1367184/150‑1 (MQ=255)
            gtttCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCg                                                                                                                                      >  2:2675971/3‑150 (MQ=255)
             gTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGt                                                                                                                                     <  1:1993491/150‑1 (MQ=255)
             gTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGt                                                                                                                                     <  1:97698/150‑1 (MQ=255)
             gTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGt                                                                                                                                     <  2:228308/150‑1 (MQ=255)
              ttCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTg                                                                                                                                    >  2:3763520/1‑150 (MQ=255)
                   cgcgTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCg                                                                                                                               <  1:2180438/150‑1 (MQ=255)
                     cgTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGt                                                                                                                             <  1:596774/150‑1 (MQ=255)
                         tCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGc                                                                                                                         >  2:205149/1‑150 (MQ=255)
                          cccGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGcc                                                                                                                        >  1:90679/1‑150 (MQ=255)
                           ccGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCg                                                                                                                       <  2:3821741/150‑1 (MQ=255)
                             ggAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCggg                                                                                                                     >  1:8429/1‑150 (MQ=255)
                             ggAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCggg                                                                                                                     <  1:3113163/150‑1 (MQ=255)
                               aaTTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCa                                                                                                                   <  1:4115393/150‑1 (MQ=255)
                                  tCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTa                                                                                                                <  1:2858330/150‑1 (MQ=255)
                                  tCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTa                                                                                                                <  1:705247/150‑1 (MQ=255)
                                    cgcgACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATc                                                                                                              <  1:4001660/150‑1 (MQ=255)
                                     gcgACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCg                                                                                                             <  1:1087577/150‑1 (MQ=255)
                                       gacgaGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGAc                                                                                                           >  1:2364612/1‑150 (MQ=255)
                                         cgaGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACaa                                                                                                         >  1:2037145/1‑150 (MQ=255)
                                          gagaACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGTTATCGACGCGCAGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCCTCGGTCTGCCGGGCACTATCGACAAc                                                                                                        >  2:2490585/1‑150 (MQ=255)
                                            gaACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGa                                                                                                      >  2:1328700/1‑150 (MQ=255)
                                             aaCATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGAc                                                                                                     >  1:2662515/1‑150 (MQ=255)
                                             aaCATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGAc                                                                                                     >  1:63713/1‑150 (MQ=255)
                                             aaCATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGAc                                                                                                     >  1:1863231/1‑150 (MQ=255)
                                                aTCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATc                                                                                                  >  1:2554110/1‑150 (MQ=255)
                                                aTCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATc                                                                                                  <  1:3305920/150‑1 (MQ=255)
                                                   cgcgCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCaaa                                                                                               >  2:2393650/1‑150 (MQ=255)
                                                   cgcgCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCaaa                                                                                               <  1:3650963/150‑1 (MQ=255)
                                                        cGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCAc                                                                                          <  2:837062/150‑1 (MQ=255)
                                                         gTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACt                                                                                         >  2:1189738/1‑150 (MQ=255)
                                                            gCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGAc                                                                                      >  2:2173970/1‑150 (MQ=255)
                                                             cTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACt                                                                                     <  1:2038332/150‑1 (MQ=255)
                                                             cTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACt                                                                                     >  1:2575873/1‑150 (MQ=255)
                                                             cTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACt                                                                                     <  2:1705655/150‑1 (MQ=255)
                                                             cTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACt                                                                                     <  1:104070/150‑1 (MQ=255)
                                                              tATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAACTGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTa                                                                                    <  2:3212482/150‑1 (MQ=255)
                                                               aTCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTac                                                                                   >  1:1334886/1‑150 (MQ=255)
                                                               aTCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTac                                                                                   >  2:1032763/1‑150 (MQ=255)
                                                                 cGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCGAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTacac                                                                                 >  1:3865436/1‑150 (MQ=255)
                                                                  gAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACt                                                                                >  2:3568789/1‑150 (MQ=255)
                                                                    aaaCCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTAt                                                                              >  2:1477130/1‑150 (MQ=255)
                                                                     aaCCTTAAAAAAAGTTGTATAGACGCGCTGGTGGTTATCGGCGTTGACGGTTCCTACTTGGGTGCAATGCGTCTGAACTAAATTGGCTTCACGTTCATCGGTCTGCCTGTCAATATCTACAACGACATCAAAGGCACTGACTACACTATc                                                                             <  2:3670078/150‑1 (MQ=255)
                                                                     aaCCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATc                                                                             <  1:1613141/150‑1 (MQ=255)
                                                                       ccTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCgg                                                                           <  2:2220773/150‑1 (MQ=255)
                                                                        cTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGt                                                                          >  1:3932505/1‑150 (MQ=255)
                                                                        cTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGt                                                                          >  1:3932485/1‑150 (MQ=255)
                                                                        cTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGt                                                                          >  1:725282/1‑150 (MQ=255)
                                                                          gAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGttt                                                                        >  1:3465000/1‑150 (MQ=255)
                                                                          gAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGttt                                                                        >  1:2265341/1‑150 (MQ=255)
                                                                           aaaacaCGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTttc                                                                       <  2:3970410/150‑1 (MQ=255)
                                                                           aaaaaaCGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCGAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTttc                                                                       >  1:2504416/1‑150 (MQ=255)
                                                                           aaaaaaCGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCGAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTttc                                                                       >  1:272604/1‑150 (MQ=255)
                                                                             aaaaCGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTttctt                                                                     <  1:2829260/150‑1 (MQ=255)
                                                                              aaaCGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTttcttc                                                                    >  1:2337021/1‑150 (MQ=255)
                                                                                   tGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTgc                                                               >  2:2438020/1‑150 (MQ=255)
                                                                                    ggTATCGACGCGCTGGTGGTTATCGGAGGTGAAGGTTCCTACATTGGTGCAATGCGACTGACCAAAAAGGGCTAACCGATCATCTGTCTGCCGGGCACTATCGACAAAGACATCAAAGGCAATGAATACACTATCGGTTTCTTCACTgcg                                                              >  2:2804124/1‑150 (MQ=255)
                                                                                     gTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTgcgc                                                             >  1:238568/1‑150 (MQ=255)
                                                                                       aTCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTg                                                           >  2:2343427/1‑150 (MQ=255)
                                                                                       aTCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTg                                                           <  1:1972638/150‑1 (MQ=255)
                                                                                        tCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGa                                                          >  2:1496541/1‑150 (MQ=255)
                                                                                          gACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGc                                                        >  2:791143/1‑150 (MQ=255)
                                                                                          gACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGc                                                        >  2:2429382/1‑150 (MQ=255)
                                                                                          gACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGc                                                        >  2:3230511/1‑150 (MQ=255)
                                                                                          gACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGc                                                        >  1:3320964/1‑150 (MQ=255)
                                                                                              cgcTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCg                                                    >  2:4036230/1‑150 (MQ=255)
                                                                                                cTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGtt                                                  <  2:2778228/150‑1 (MQ=255)
                                                                                                         aTCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCg                                         >  1:1427750/1‑150 (MQ=255)
                                                                                                           cggcggTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGAt                                       <  2:3535441/150‑1 (MQ=255)
                                                                                                               ggTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGAc                                   >  2:3439252/1‑150 (MQ=255)
                                                                                                                gTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGAcc                                  >  1:3620204/1‑150 (MQ=255)
                                                                                                                gTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGAcc                                  <  1:3802226/150‑1 (MQ=255)
                                                                                                                   aCGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTc                               >  1:1149019/1‑150 (MQ=255)
                                                                                                                    cGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCt                              >  2:544342/1‑150 (MQ=255)
                                                                                                                     ggTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTg                             <  2:2163768/150‑1 (MQ=255)
                                                                                                                     ggTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTg                             >  1:2203518/1‑150 (MQ=255)
                                                                                                                     ggTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTg                             >  2:1869698/1‑150 (MQ=255)
                                                                                                                      gTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGc                            <  2:181521/150‑1 (MQ=255)
                                                                                                                      gTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGc                            <  2:611459/150‑1 (MQ=255)
                                                                                                                       ttCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCg                           >  1:808153/1‑150 (MQ=255)
                                                                                                                             cATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGacac                     >  2:2551595/1‑150 (MQ=255)
                                                                                                                              aTGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACAcc                    >  2:295112/1‑150 (MQ=255)
                                                                                                                              aTGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACAcc                    >  1:3151211/1‑150 (MQ=255)
                                                                                                                                  gTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCtctt                <  2:6767/150‑1 (MQ=255)
                                                                                                                                   tGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCtcttc               <  1:2908983/150‑1 (MQ=255)
                                                                                                                                     cAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCtcttctt             >  2:340938/1‑150 (MQ=255)
                                                                                                                                     cAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCtcttctt             <  2:2896841/150‑1 (MQ=255)
                                                                                                                                      aaTGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCtcttcttc            <  1:1236305/150‑1 (MQ=255)
                                                                                                                                       aTGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCtcttcttct           <  1:2935176/150‑1 (MQ=255)
                                                                                                                                            tCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCTCTTCTTCTCACCa      <  1:3380153/150‑1 (MQ=255)
                                                                                                                                            tCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCTCTTCTTCTCACCa      <  1:1631799/150‑1 (MQ=255)
                                                                                                                                              tGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCTCTTCTTCTCACCAGc    >  2:1210095/1‑150 (MQ=255)
                                                                                                                                              tGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCTCTTCTTCTCACCAGc    <  1:3985347/150‑1 (MQ=255)
                                                                                                                                              tGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCTCTTCTTCTCACCAGc    <  2:735735/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGTACGTTCCTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCGAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCTCTTCTTCTCACCAGCGT  >  CP009273/4097675‑4097968

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGTACGTTCCTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCGAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCTCTTCTTCTCACCAGCGT  >  CP009273/4097675‑4097968
                                                                                                                                                   |                                                                                                                                                  
GGTACGTTCCTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAA                                                                                                                                                  >  SRR19973074.4112634/1‑150 (MQ=60)
    CGTTCCTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGG                                                                                                                                              <  SRR19973074.4136322/150‑1 (MQ=60)
     GTTCCTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGG                                                                                                                                             >  SRR19973074.2588075/1‑150 (MQ=60)
     GTTCCTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGG                                                                                                                                             >  SRR19973074.1864169/1‑150 (MQ=60)
        CCTCGGTTCTGCTCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTT                                                                                                                                          >  SRR19973074.2475372/1‑150 (MQ=60)
          TCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCC                                                                                                                                        <  SRR19973074.1075252/150‑1 (MQ=60)
            GGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCG                                                                                                                                      >  SRR19973074.3789678/1‑150 (MQ=60)
            GGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCG                                                                                                                                      >  SRR19973074.3153848/1‑150 (MQ=60)
             GTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGT                                                                                                                                     <  SRR19973074.97939/150‑1 (MQ=60)
             GTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGT                                                                                                                                     <  SRR19973074.1997364/150‑1 (MQ=60)
             GTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGT                                                                                                                                     <  SRR19973074.1997275/150‑1 (MQ=60)
                CTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCA                                                                                                                                  <  SRR19973074.3638126/150‑1 (MQ=60)
                   CGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCG                                                                                                                               >  SRR19973074.2475111/1‑150 (MQ=60)
                   CGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCG                                                                                                                               <  SRR19973074.2184535/150‑1 (MQ=60)
                   CGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCG                                                                                                                               <  SRR19973074.194818/150‑1 (MQ=60)
                   CGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCG                                                                                                                               <  SRR19973074.194813/150‑1 (MQ=60)
                     CGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGT                                                                                                                             <  SRR19973074.597919/150‑1 (MQ=60)
                     CGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGT                                                                                                                             <  SRR19973074.2986455/150‑1 (MQ=60)
                       TTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCT                                                                                                                           <  SRR19973074.2886103/150‑1 (MQ=60)
                       TTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCT                                                                                                                           <  SRR19973074.1432924/150‑1 (MQ=60)
                          CCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCC                                                                                                                        >  SRR19973074.90902/1‑150 (MQ=60)
                           CCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCG                                                                                                                       <  SRR19973074.236204/150‑1 (MQ=60)
                            CGGAATTCCGCGACGAGAACATCAGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGG                                                                                                                      >  SRR19973074.2476008/1‑150 (MQ=60)
                             GGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGG                                                                                                                     >  SRR19973074.8452/1‑150 (MQ=60)
                             GGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGG                                                                                                                     <  SRR19973074.3118874/150‑1 (MQ=60)
                               AATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCA                                                                                                                   <  SRR19973074.4122850/150‑1 (MQ=60)
                               AATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCA                                                                                                                   >  SRR19973074.1286266/1‑150 (MQ=60)
                               AATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCA                                                                                                                   <  SRR19973074.4122987/150‑1 (MQ=60)
                                  TCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTA                                                                                                                <  SRR19973074.2863634/150‑1 (MQ=60)
                                  TCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTA                                                                                                                <  SRR19973074.706550/150‑1 (MQ=60)
                                   CCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTAT                                                                                                               <  SRR19973074.344941/150‑1 (MQ=60)
                                    CGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATC                                                                                                              >  SRR19973074.3991646/1‑150 (MQ=60)
                                    CGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATC                                                                                                              <  SRR19973074.4009050/150‑1 (MQ=60)
                                     GCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCG                                                                                                             <  SRR19973074.1089616/150‑1 (MQ=60)
                                       GACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGAC                                                                                                           >  SRR19973074.2368953/1‑150 (MQ=60)
                                       GACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGAC                                                                                                           >  SRR19973074.2369014/1‑150 (MQ=60)
                                         CGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAA                                                                                                         >  SRR19973074.2040985/1‑150 (MQ=60)
                                          GAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAAC                                                                                                        >  SRR19973074.4292/1‑150 (MQ=60)
                                          GAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAAC                                                                                                        <  SRR19973074.3022892/150‑1 (MQ=60)
                                           AGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACG                                                                                                       >  SRR19973074.2263555/1‑150 (MQ=60)
                                             AACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGAC                                                                                                     >  SRR19973074.2667476/1‑150 (MQ=60)
                                             AACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGAC                                                                                                     >  SRR19973074.63879/1‑150 (MQ=60)
                                             AACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGAC                                                                                                     >  SRR19973074.1866791/1‑150 (MQ=60)
                                             AACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGAC                                                                                                     >  SRR19973074.1866785/1‑150 (MQ=60)
                                               CATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACAT                                                                                                   >  SRR19973074.773304/1‑150 (MQ=60)
                                                ATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATC                                                                                                  >  SRR19973074.2558877/1‑150 (MQ=60)
                                                ATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATC                                                                                                  <  SRR19973074.3312053/150‑1 (MQ=60)
                                                   CGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAA                                                                                               >  SRR19973074.1387549/1‑150 (MQ=60)
                                                   CGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAA                                                                                               <  SRR19973074.3657720/150‑1 (MQ=60)
                                                   CGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAA                                                                                               <  SRR19973074.3657795/150‑1 (MQ=60)
                                                     CGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGG                                                                                             >  SRR19973074.285928/1‑150 (MQ=60)
                                                       CCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCA                                                                                           >  SRR19973074.2520549/1‑150 (MQ=60)
                                                        CGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCAC                                                                                          >  SRR19973074.2719401/1‑150 (MQ=60)
                                                         GTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACT                                                                                         >  SRR19973074.2909791/1‑150 (MQ=60)
                                                             CTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACT                                                                                     <  SRR19973074.2042173/150‑1 (MQ=60)
                                                             CTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACT                                                                                     <  SRR19973074.2042144/150‑1 (MQ=60)
                                                             CTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACT                                                                                     >  SRR19973074.1457334/1‑150 (MQ=60)
                                                             CTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACT                                                                                     >  SRR19973074.2580682/1‑150 (MQ=60)
                                                             CTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACT                                                                                     <  SRR19973074.104324/150‑1 (MQ=60)
                                                               ATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTAC                                                                                   >  SRR19973074.1337381/1‑150 (MQ=60)
                                                                 CGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCGAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACAC                                                                                 >  SRR19973074.3872501/1‑150 (MQ=60)
                                                                    AAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTAT                                                                              >  SRR19973074.3951772/1‑150 (MQ=60)
                                                                    AAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTAT                                                                              >  SRR19973074.2193465/1‑150 (MQ=60)
                                                                     AACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATC                                                                             <  SRR19973074.1616207/150‑1 (MQ=60)
                                                                     AACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATC                                                                             <  SRR19973074.2215835/150‑1 (MQ=60)
                                                                        CTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGT                                                                          <  SRR19973074.940123/150‑1 (MQ=60)
                                                                        CTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGT                                                                          >  SRR19973074.3939745/1‑150 (MQ=60)
                                                                        CTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGT                                                                          >  SRR19973074.3939744/1‑150 (MQ=60)
                                                                        CTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGT                                                                          >  SRR19973074.3939725/1‑150 (MQ=60)
                                                                        CTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGT                                                                          >  SRR19973074.215913/1‑150 (MQ=60)
                                                                        CTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGT                                                                          >  SRR19973074.726644/1‑150 (MQ=60)
                                                                        CTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGT                                                                          <  SRR19973074.2662003/150‑1 (MQ=60)
                                                                         TGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTT                                                                         <  SRR19973074.2002095/150‑1 (MQ=60)
                                                                          GAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTT                                                                        >  SRR19973074.3471367/1‑150 (MQ=60)
                                                                          GAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTT                                                                        >  SRR19973074.2269594/1‑150 (MQ=60)
                                                                           AAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCGAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTC                                                                       >  SRR19973074.2509079/1‑150 (MQ=60)
                                                                           AAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCGAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTC                                                                       >  SRR19973074.273128/1‑150 (MQ=60)
                                                                            AAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCT                                                                      <  SRR19973074.128148/150‑1 (MQ=60)
                                                                             AAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTT                                                                     <  SRR19973074.3317458/150‑1 (MQ=60)
                                                                             AAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTT                                                                     <  SRR19973074.2834494/150‑1 (MQ=60)
                                                                              AAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTC                                                                    >  SRR19973074.2341380/1‑150 (MQ=60)
                                                                              AAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTC                                                                    <  SRR19973074.3568176/150‑1 (MQ=60)
                                                                                  GTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTG                                                                >  SRR19973074.720188/1‑150 (MQ=60)
                                                                                  GTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTG                                                                <  SRR19973074.2371561/150‑1 (MQ=60)
                                                                                     GTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGC                                                             >  SRR19973074.239052/1‑150 (MQ=60)
                                                                                       ATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTG                                                           <  SRR19973074.1976396/150‑1 (MQ=60)
                                                                                         CGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAG                                                         >  SRR19973074.683810/1‑150 (MQ=60)
                                                                                          GACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGC                                                        >  SRR19973074.4086004/1‑150 (MQ=60)
                                                                                          GACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGC                                                        >  SRR19973074.3327141/1‑150 (MQ=60)
                                                                                          GACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGC                                                        >  SRR19973074.3327129/1‑150 (MQ=60)
                                                                                              CGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCG                                                    >  SRR19973074.602867/1‑150 (MQ=60)
                                                                                                 TGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTG                                                 >  SRR19973074.2742212/1‑150 (MQ=60)
                                                                                                 TGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTG                                                 >  SRR19973074.1110685/1‑150 (MQ=60)
                                                                                                 TGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTG                                                 >  SRR19973074.2742276/1‑150 (MQ=60)
                                                                                                  GGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGT                                                >  SRR19973074.2050042/1‑150 (MQ=60)
                                                                                                  GGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGT                                                <  SRR19973074.3886735/150‑1 (MQ=60)
                                                                                                      GTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAA                                            <  SRR19973074.84526/150‑1 (MQ=60)
                                                                                                      GTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAA                                            <  SRR19973074.807408/150‑1 (MQ=60)
                                                                                                         ATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCG                                         >  SRR19973074.1430394/1‑150 (MQ=60)
                                                                                                         ATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCG                                         <  SRR19973074.1621128/150‑1 (MQ=60)
                                                                                                          TCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGA                                        <  SRR19973074.948468/150‑1 (MQ=60)
                                                                                                          TCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGA                                        >  SRR19973074.1685974/1‑150 (MQ=60)
                                                                                                            GGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATC                                      <  SRR19973074.2470246/150‑1 (MQ=60)
                                                                                                            GGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATC                                      <  SRR19973074.1179020/150‑1 (MQ=60)
                                                                                                             GCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCG                                     <  SRR19973074.1138147/150‑1 (MQ=60)
                                                                                                               GGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGAC                                   >  SRR19973074.384875/1‑150 (MQ=60)
                                                                                                                GTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACC                                  <  SRR19973074.3809185/150‑1 (MQ=60)
                                                                                                                GTGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACC                                  >  SRR19973074.3626890/1‑150 (MQ=60)
                                                                                                                 TGACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCG                                 >  SRR19973074.3548823/1‑150 (MQ=60)
                                                                                                                  GACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGT                                <  SRR19973074.3234963/150‑1 (MQ=60)
                                                                                                                   ACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTC                               >  SRR19973074.317757/1‑150 (MQ=60)
                                                                                                                   ACGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTC                               >  SRR19973074.1151211/1‑150 (MQ=60)
                                                                                                                    CGGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCT                              <  SRR19973074.2559200/150‑1 (MQ=60)
                                                                                                                     GGTTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTG                             >  SRR19973074.2207663/1‑150 (MQ=60)
                                                                                                                       TTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCG                           >  SRR19973074.1988524/1‑150 (MQ=60)
                                                                                                                       TTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCG                           >  SRR19973074.809731/1‑150 (MQ=60)
                                                                                                                       TTCCTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCG                           >  SRR19973074.809709/1‑150 (MQ=60)
                                                                                                                          CTACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACAGCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGA                        >  SRR19973074.844112/1‑150 (MQ=60)
                                                                                                                            ACATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACA                      >  SRR19973074.3375190/1‑150 (MQ=60)
                                                                                                                             CATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACAC                     <  SRR19973074.2339006/150‑1 (MQ=60)
                                                                                                                              ATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACC                    >  SRR19973074.4002787/1‑150 (MQ=60)
                                                                                                                              ATGGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACC                    >  SRR19973074.3156997/1‑150 (MQ=60)
                                                                                                                                GGGTGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCTC                  >  SRR19973074.653680/1‑150 (MQ=60)
                                                                                                                                   TGCAATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCTCTTC               <  SRR19973074.2914384/150‑1 (MQ=60)
                                                                                                                                      AATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCTCTTCTTC            <  SRR19973074.1238672/150‑1 (MQ=60)
                                                                                                                                       ATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCTCTTCTTCT           <  SRR19973074.2940634/150‑1 (MQ=60)
                                                                                                                                       ATGCGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCTCTTCTTCT           <  SRR19973074.2940635/150‑1 (MQ=60)
                                                                                                                                          CGTCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCTCTTCTTCTCAC        >  SRR19973074.3892023/1‑150 (MQ=60)
                                                                                                                                            TCTGACCTAAATGGGCTTCCCGTGCTTCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTAACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCTCTTCTTCTCACCA      <  SRR19973074.2553675/150‑1 (MQ=60)
                                                                                                                                            TCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCTCTTCTTCTCACCA      <  SRR19973074.1634894/150‑1 (MQ=60)
                                                                                                                                            TCTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCTCTTCTTCTCACCA      <  SRR19973074.3386412/150‑1 (MQ=60)
                                                                                                                                             CTGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCTCTTCTTCTCACCAG     >  SRR19973074.4012011/1‑150 (MQ=60)
                                                                                                                                              TGACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCTCTTCTTCTCACCAGC    <  SRR19973074.3992700/150‑1 (MQ=60)
                                                                                                                                                ACCTAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCTCTTCTTCTCACCAGCGT  >  SRR19973074.1772027/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                  
GGTACGTTCCTCGGTTCTGCGCGTTTCCCGGAATTCCGCGACGAGAACATCCGCGCCGTGGCTATCGAAAACCTGAAAAAACGTGGTATCGACGCGCTGGTGGTTATCGGCGGTGACGGTTCCTACATGGGTGCAATGCGTCTGACCGAAATGGGCTTCCCGTGCATCGGTCTGCCGGGCACTATCGACAACGACATCAAAGGCACTGACTACACTATCGGTTTCTTCACTGCGCTGAGCACCGTTGTAGAAGCGATCGACCGTCTGCGTGACACCTCTTCTTCTCACCAGCGT  >  CP009273/4097675‑4097968

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑