Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F78 I0 R1 626 241.3 10389380 77.7% 8072548 149.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,711,397 C→T 95.0% R28Q (CGA→CAA)  tyrS ← tyrosyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,711,3970CT95.0% 851.8 / 21.4 257R28Q (CGA→CAA) tyrStyrosyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (5/8);  new base T (104/140);  total (109/148)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.33e-01

Only 100 of 257 total aligned reads displayed.

CCGTCGCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAA  >  CP009273/1711251‑1711543
                                                                                                                                                  |                                                                                                                                                  
ccGTCGCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGct                                                                                                                                                 >  1:3854137/1‑150 (MQ=255)
 cGTCGCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGctc                                                                                                                                                <  1:2491860/150‑1 (MQ=255)
 cGTCGCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGctc                                                                                                                                                <  1:5071144/150‑1 (MQ=255)
     gcgcCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCt                                                                                                                                            >  1:1679868/1‑150 (MQ=255)
       gccgccTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGGTGCTCTGCTaa                                                                                                                                          >  1:355821/1‑150 (MQ=255)
        ccgccTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAAc                                                                                                                                         <  2:1307025/150‑1 (MQ=255)
        ccgccTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAAc                                                                                                                                         <  1:1568586/150‑1 (MQ=255)
         cgccTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACg                                                                                                                                        <  1:86209/150‑1 (MQ=255)
          gccTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGc                                                                                                                                       <  2:3386422/150‑1 (MQ=255)
            cTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCtt                                                                                                                                     >  2:335395/1‑150 (MQ=255)
            cTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCtt                                                                                                                                     >  2:1371601/1‑150 (MQ=255)
                    gcAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTcgtcc                                                                                                                             <  2:1049028/150‑1 (MQ=255)
                     cAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTcgtccg                                                                                                                            >  2:2079285/1‑150 (MQ=255)
                       aCCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTcgtccgtc                                                                                                                          <  2:5060106/150‑1 (MQ=255)
                       aCCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTcgtccgtc                                                                                                                          <  1:2898704/150‑1 (MQ=255)
                          ggCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCAcc                                                                                                                       <  2:2972471/150‑1 (MQ=255)
                           gCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCt                                                                                                                      <  1:3681496/150‑1 (MQ=255)
                              tgtgGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTggg                                                                                                                   <  1:910921/150‑1 (MQ=255)
                                tgGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCt                                                                                                                 <  1:2835626/150‑1 (MQ=255)
                                 ggCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTa                                                                                                                >  2:4547136/1‑150 (MQ=255)
                                   cccGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTAcc                                                                                                              >  1:4390523/1‑150 (MQ=255)
                                    ccGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCa                                                                                                             <  1:1064678/150‑1 (MQ=255)
                                     cGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAg                                                                                                            <  2:1906434/150‑1 (MQ=255)
                                        ctgctgGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGccc                                                                                                         >  2:3838750/1‑150 (MQ=255)
                                         tgctgGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGTCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGcccc                                                                                                        >  2:544650/1‑150 (MQ=255)
                                            tgGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGc                                                                                                     >  2:286827/1‑150 (MQ=255)
                                             ggAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGct                                                                                                    >  1:4841710/1‑150 (MQ=255)
                                               aaGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGctct                                                                                                  <  1:2711336/150‑1 (MQ=255)
                                                aGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCtt                                                                                                 >  2:4966908/1‑150 (MQ=255)
                                                aGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCtt                                                                                                 <  2:3974558/150‑1 (MQ=255)
                                                   gTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCa                                                                                              >  1:4579471/1‑150 (MQ=255)
                                                     ttCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAAt                                                                                            <  2:2379254/150‑1 (MQ=255)
                                                     ttCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAAt                                                                                            >  1:3808022/1‑150 (MQ=255)
                                                     ttCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAAt                                                                                            <  1:2614388/150‑1 (MQ=255)
                                                       cAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGGTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTg                                                                                          <  1:191246/150‑1 (MQ=255)
                                                         ggCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGtt                                                                                        <  1:3151183/150‑1 (MQ=255)
                                                            aTAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTaa                                                                                     >  1:2731229/1‑150 (MQ=255)
                                                              aaCAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATc                                                                                   >  1:2291470/1‑150 (MQ=255)
                                                              aaCAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATc                                                                                   <  2:425462/150‑1 (MQ=255)
                                                               aCAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCa                                                                                  >  1:1091839/1‑150 (MQ=255)
                                                               aCAATGGAACAAGATGCCCCAAAGGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCa                                                                                  <  1:3127587/150‑1 (MQ=255)
                                                                 aaTGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAg                                                                                >  1:3306654/1‑150 (MQ=255)
                                                                  aTGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGt                                                                               >  2:2281336/1‑150 (MQ=255)
                                                                    ggAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTa                                                                             <  2:1364382/150‑1 (MQ=255)
                                                                      aaCAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACt                                                                           >  2:1621519/1‑150 (MQ=255)
                                                                      aaCAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACt                                                                           >  1:578823/1‑150 (MQ=255)
                                                                      aaCAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACt                                                                           >  2:3320897/1‑150 (MQ=255)
                                                                          aGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCtt                                                                       <  2:1161365/150‑1 (MQ=255)
                                                                           gATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTg                                                                      >  1:1398238/1‑150 (MQ=255)
                                                                              gCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCa                                                                   >  2:1725183/1‑150 (MQ=255)
                                                                               ccccAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCAt                                                                  <  1:3076682/150‑1 (MQ=255)
                                                                                 ccAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCa                                                                >  2:134613/1‑150 (MQ=255)
                                                                                    aaTGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCaaaa                                                             >  2:3680002/1‑150 (MQ=255)
                                                                                    aaTGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCaaaa                                                             <  2:2963454/150‑1 (MQ=255)
                                                                                     aTGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAAt                                                            <  2:3262205/150‑1 (MQ=255)
                                                                                      tGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAAtc                                                           <  1:3599959/150‑1 (MQ=255)
                                                                                       gCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAAtct                                                          <  2:4682643/150‑1 (MQ=255)
                                                                                       gCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAAtct                                                          <  2:4682654/150‑1 (MQ=255)
                                                                                       gCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAAtct                                                          >  2:4797541/1‑150 (MQ=255)
                                                                                            cTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATg                                                     <  2:2517904/150‑1 (MQ=255)
                                                                                              gTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGta                                                   >  1:3930408/1‑150 (MQ=255)
                                                                                              gTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGta                                                   >  2:761871/1‑150 (MQ=255)
                                                                                               tCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGtat                                                  <  1:2046679/150‑1 (MQ=255)
                                                                                               tCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGtat                                                  <  2:4958797/150‑1 (MQ=255)
                                                                                                  gCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGtatata                                               <  1:1775727/150‑1 (MQ=255)
                                                                                                    ggTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAAc                                             >  2:4929082/1‑150 (MQ=255)
                                                                                                     gTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACg                                            >  2:402574/1‑150 (MQ=255)
                                                                                                       aGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGAc                                          >  2:2023403/1‑150 (MQ=255)
                                                                                                        ggATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACt                                         <  1:488815/150‑1 (MQ=255)
                                                                                                        ggATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACt                                         <  2:1399621/150‑1 (MQ=255)
                                                                                                         gATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTg                                        <  2:4475497/150‑1 (MQ=255)
                                                                                                          aTCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGc                                       <  2:5146981/150‑1 (MQ=255)
                                                                                                          aTCGAAGCCGAAATAGCGCGCGATCGGGCCTTGCGCCTGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGATACCAGCCCCCGATCTTGAATTTGTTTAATAAATTTAGTGATTGCCATAAAAATCTCCTTTTATATAACGACTGc                                       <  1:1369149/150‑1 (MQ=255)
                                                                                                           tCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCa                                      >  1:2423134/1‑150 (MQ=255)
                                                                                                            cGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCAc                                     >  2:301936/1‑150 (MQ=255)
                                                                                                               aGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCtt                                  <  2:4383553/150‑1 (MQ=255)
                                                                                                               aGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGAAATTGTTTAATCATGTTACTGCTTGCCATCAAAATCTCCTTGTATATAACGACTGCACCtt                                  <  1:335395/150‑1 (MQ=255)
                                                                                                                gCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCttt                                 >  1:620656/1‑150 (MQ=255)
                                                                                                                gCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCttt                                 <  2:355821/150‑1 (MQ=255)
                                                                                                                 ccGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTATTGAAATTGTTTAATCAATTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTg                                <  1:1240541/150‑1 (MQ=255)
                                                                                                                  cGCAATAGTGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGc                               <  2:1754628/150‑1 (MQ=255)
                                                                                                                        agagCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTac                         <  2:3605401/150‑1 (MQ=255)
                                                                                                                         gagCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTcca                        >  1:605424/1‑150 (MQ=255)
                                                                                                                          agCGCGATCGGGCCTTGCGCCTGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAATTTACGGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTacac                       <  1:3536737/150‑1 (MQ=255)
                                                                                                                               gATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACtt                  >  1:4625910/1‑150 (MQ=255)
                                                                                                                                  cGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTc               >  1:4132812/1‑150 (MQ=255)
                                                                                                                                  cGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTc               <  1:4706168/150‑1 (MQ=255)
                                                                                                                                     gCCTTGCGCCAGGTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAAGTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGcc            <  2:164208/150‑1 (MQ=255)
                                                                                                                                       cTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAg          >  1:3181814/1‑150 (MQ=255)
                                                                                                                                       cTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAg          >  1:4977226/1‑150 (MQ=255)
                                                                                                                                        ttGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGa         >  1:3282347/1‑150 (MQ=255)
                                                                                                                                         tGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGAt        <  2:1231923/150‑1 (MQ=255)
                                                                                                                                         tGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGAt        >  1:3840253/1‑150 (MQ=255)
                                                                                                                                         tGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGAt        <  2:3654150/150‑1 (MQ=255)
                                                                                                                                          gcgcCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATg       >  2:1868875/1‑150 (MQ=255)
                                                                                                                                            gcCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTCTATAACGACTGCACCGTTGCCGGTACACGACTTTTCGCCAGATGCg     >  1:561698/1‑150 (MQ=255)
                                                                                                                                            gcCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCg     >  1:4243690/1‑150 (MQ=255)
                                                                                                                                            gcCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCg     >  2:2608537/1‑150 (MQ=255)
                                                                                                                                             ccAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTTTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGa    <  1:3937338/150‑1 (MQ=255)
                                                                                                                                             ccAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGa    <  2:2670359/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CCGTCGCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAA  >  CP009273/1711251‑1711543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCGTCGCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGA  >  CP009273/1711251‑1711545
                                                                                                                                                  |                                                                                                                                                    
CCGTCGCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCT                                                                                                                                                   >  SRR19973083.3871576/1‑150 (MQ=60)
CCGTCGCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCT                                                                                                                                                   >  SRR19973083.3871406/1‑150 (MQ=60)
 CGTCGCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTC                                                                                                                                                  >  SRR19973083.1112135/1‑150 (MQ=60)
 CGTCGCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTC                                                                                                                                                  <  SRR19973083.5092840/150‑1 (MQ=60)
 CGTCGCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTC                                                                                                                                                  <  SRR19973083.2504574/150‑1 (MQ=60)
     GCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCT                                                                                                                                              >  SRR19973083.1688175/1‑150 (MQ=60)
       GCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAA                                                                                                                                            >  SRR19973083.357508/1‑150 (MQ=60)
       GCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGGTGCTCTGCTAA                                                                                                                                            >  SRR19973083.357507/1‑150 (MQ=60)
        CCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAAC                                                                                                                                           <  SRR19973083.1576460/150‑1 (MQ=60)
         CGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACG                                                                                                                                          <  SRR19973083.86812/150‑1 (MQ=60)
           CCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCT                                                                                                                                        <  SRR19973083.3306575/150‑1 (MQ=60)
           CCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCT                                                                                                                                        <  SRR19973083.2571374/150‑1 (MQ=60)
            CTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTT                                                                                                                                       >  SRR19973083.2676915/1‑150 (MQ=60)
            CTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTT                                                                                                                                       <  SRR19973083.2497405/150‑1 (MQ=60)
             TACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGATTC                                                                                                                                      <  SRR19973083.224772/150‑1 (MQ=60)
                     CAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCG                                                                                                                              >  SRR19973083.2935798/1‑150 (MQ=60)
                       ACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTC                                                                                                                            <  SRR19973083.3864554/150‑1 (MQ=60)
                       ACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTC                                                                                                                            <  SRR19973083.2912911/150‑1 (MQ=60)
                          GGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACC                                                                                                                         <  SRR19973083.3321111/150‑1 (MQ=60)
                           GCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCT                                                                                                                        <  SRR19973083.3322325/150‑1 (MQ=60)
                           GCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCT                                                                                                                        <  SRR19973083.3698289/150‑1 (MQ=60)
                           GCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCT                                                                                                                        >  SRR19973083.1380777/1‑150 (MQ=60)
                           GCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCT                                                                                                                        >  SRR19973083.1380768/1‑150 (MQ=60)
                            CTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTG                                                                                                                       <  SRR19973083.2805121/150‑1 (MQ=60)
                            CTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTG                                                                                                                       <  SRR19973083.2804424/150‑1 (MQ=60)
                              TGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGG                                                                                                                     <  SRR19973083.915290/150‑1 (MQ=60)
                               GTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGC                                                                                                                    <  SRR19973083.3651510/150‑1 (MQ=60)
                                TGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCT                                                                                                                   <  SRR19973083.2849649/150‑1 (MQ=60)
                                   CCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACC                                                                                                                <  SRR19973083.1315230/150‑1 (MQ=60)
                                   CCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACC                                                                                                                >  SRR19973083.4409616/1‑150 (MQ=60)
                                    CCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCA                                                                                                               <  SRR19973083.1070039/150‑1 (MQ=60)
                                     CGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAG                                                                                                              <  SRR19973083.787676/150‑1 (MQ=60)
                                        CTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCC                                                                                                           >  SRR19973083.1613554/1‑150 (MQ=60)
                                          GCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCC                                                                                                         <  SRR19973083.1523540/150‑1 (MQ=60)
                                             GGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCT                                                                                                      >  SRR19973083.4862496/1‑150 (MQ=60)
                                               AAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCT                                                                                                    <  SRR19973083.2724895/150‑1 (MQ=60)
                                               AAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCT                                                                                                    <  SRR19973083.2724929/150‑1 (MQ=60)
                                               AAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCT                                                                                                    <  SRR19973083.2724956/150‑1 (MQ=60)
                                               AAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCT                                                                                                    >  SRR19973083.4478245/1‑150 (MQ=60)
                                                AGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTT                                                                                                   <  SRR19973083.1644237/150‑1 (MQ=60)
                                                   GTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCA                                                                                                >  SRR19973083.2026250/1‑150 (MQ=60)
                                                   GTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCA                                                                                                >  SRR19973083.4599271/1‑150 (MQ=60)
                                                   GTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCA                                                                                                >  SRR19973083.4623641/1‑150 (MQ=60)
                                                     TTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAAT                                                                                              <  SRR19973083.2627527/150‑1 (MQ=60)
                                                     TTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAAT                                                                                              >  SRR19973083.3825147/1‑150 (MQ=60)
                                                      TCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATT                                                                                             >  SRR19973083.3597927/1‑150 (MQ=60)
                                                       CAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGGTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTG                                                                                            <  SRR19973083.192323/150‑1 (MQ=60)
                                                         GGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTT                                                                                          <  SRR19973083.3166200/150‑1 (MQ=60)
                                                         GGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTT                                                                                          <  SRR19973083.3166202/150‑1 (MQ=60)
                                                         GGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTT                                                                                          <  SRR19973083.3166230/150‑1 (MQ=60)
                                                            ATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAA                                                                                       >  SRR19973083.1883026/1‑150 (MQ=60)
                                                            ATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAA                                                                                       >  SRR19973083.5063485/1‑150 (MQ=60)
                                                            ATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAA                                                                                       >  SRR19973083.2744868/1‑150 (MQ=60)
                                                             TAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAAT                                                                                      <  SRR19973083.2568068/150‑1 (MQ=60)
                                                             TAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAAT                                                                                      <  SRR19973083.1156241/150‑1 (MQ=60)
                                                              AACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATC                                                                                     >  SRR19973083.2303209/1‑150 (MQ=60)
                                                               ACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCA                                                                                    <  SRR19973083.3826191/150‑1 (MQ=60)
                                                               ACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCA                                                                                    >  SRR19973083.3106119/1‑150 (MQ=60)
                                                               ACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCA                                                                                    >  SRR19973083.1097335/1‑150 (MQ=60)
                                                               ACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTAGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGAAATTGTTTAATCA                                                                                    <  SRR19973083.3826185/150‑1 (MQ=60)
                                                               ACAATGGAACAAGATGCCCCAAAGGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCA                                                                                    <  SRR19973083.3142546/150‑1 (MQ=60)
                                                                 AATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAG                                                                                  >  SRR19973083.3322182/1‑150 (MQ=60)
                                                                     GAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTAC                                                                              <  SRR19973083.1771612/150‑1 (MQ=60)
                                                                     GAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTAC                                                                              <  SRR19973083.693924/150‑1 (MQ=60)
                                                                      AACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACT                                                                             <  SRR19973083.1086489/150‑1 (MQ=60)
                                                                      AACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACT                                                                             >  SRR19973083.581718/1‑150 (MQ=60)
                                                                       ACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTG                                                                            <  SRR19973083.2345573/150‑1 (MQ=60)
                                                                           GATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTG                                                                        >  SRR19973083.1405212/1‑150 (MQ=60)
                                                                           GATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTG                                                                        <  SRR19973083.2872541/150‑1 (MQ=60)
                                                                               CCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCAT                                                                    <  SRR19973083.469473/150‑1 (MQ=60)
                                                                               CCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCAT                                                                    <  SRR19973083.3091430/150‑1 (MQ=60)
                                                                               CCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCAT                                                                    <  SRR19973083.3091423/150‑1 (MQ=60)
                                                                                  CAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCTTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCCTGCCATCAA                                                                 >  SRR19973083.3209362/1‑150 (MQ=60)
                                                                                     ATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAAT                                                              >  SRR19973083.3824867/1‑150 (MQ=60)
                                                                                     ATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAAT                                                              >  SRR19973083.3824914/1‑150 (MQ=60)
                                                                                      TGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATC                                                             <  SRR19973083.3616446/150‑1 (MQ=60)
                                                                                       GCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCT                                                            <  SRR19973083.4255870/150‑1 (MQ=60)
                                                                                       GCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCT                                                            <  SRR19973083.5152604/150‑1 (MQ=60)
                                                                                            CTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATG                                                       <  SRR19973083.1939765/150‑1 (MQ=60)
                                                                                              GTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTA                                                     >  SRR19973083.3947985/1‑150 (MQ=60)
                                                                                              GTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTA                                                     >  SRR19973083.3947905/1‑150 (MQ=60)
                                                                                               TCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTAT                                                    <  SRR19973083.2057124/150‑1 (MQ=60)
                                                                                                  GCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATA                                                 <  SRR19973083.1784560/150‑1 (MQ=60)
                                                                                                       AGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGAC                                            >  SRR19973083.4653352/1‑150 (MQ=60)
                                                                                                        GGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACT                                           <  SRR19973083.1880334/150‑1 (MQ=60)
                                                                                                        GGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACT                                           <  SRR19973083.491174/150‑1 (MQ=60)
                                                                                                        GGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTATTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACT                                           <  SRR19973083.490596/150‑1 (MQ=60)
                                                                                                          ATCGAAGCCGAAATAGCGCGCGATCGGGCCTTGCGCCTGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGATACCAGCCCCCGATCTTGAATTTGTTTAATAAATTTAGTGATTGCCATAAAAATCTCCTTTTATATAACGACTGC                                         <  SRR19973083.1375923/150‑1 (MQ=60)
                                                                                                           TCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCA                                        >  SRR19973083.2435573/1‑150 (MQ=60)
                                                                                                            CGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCAC                                       >  SRR19973083.599199/1‑150 (MQ=60)
                                                                                                             GAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACC                                      <  SRR19973083.3411869/150‑1 (MQ=60)
                                                                                                               AGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGAAATTGTTTAATCATGTTACTGCTTGCCATCAAAATCTCCTTGTATATAACGACTGCACCTT                                    <  SRR19973083.337036/150‑1 (MQ=60)
                                                                                                                GCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTT                                   >  SRR19973083.623064/1‑150 (MQ=60)
                                                                                                                GCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTT                                   >  SRR19973083.623767/1‑150 (MQ=60)
                                                                                                                 CCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTATTGAAATTGTTTAATCAATTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTG                                  <  SRR19973083.1246609/150‑1 (MQ=60)
                                                                                                                         GAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCTTGTATATAACGACTGCACCTTTGCCGGTACA                          <  SRR19973083.596718/150‑1 (MQ=60)
                                                                                                                         GAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTCCA                          >  SRR19973083.608459/1‑150 (MQ=60)
                                                                                                                         GAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACA                          >  SRR19973083.986736/1‑150 (MQ=60)
                                                                                                                          AGCGCGATCGGGCCTTGCGCCTGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAATTTACGGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACAC                         <  SRR19973083.3552976/150‑1 (MQ=60)
                                                                                                                          AGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCTTCACCTGGGCTACCAGCCCCCGCTCTTGAAATTTTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACAC                         <  SRR19973083.3552978/150‑1 (MQ=60)
                                                                                                                             GCTATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCAACCAGCCCCCGCTCTTTCAATTGTTTAAACAAGTTACTGCTTGCCAACAAAATCTCCATTTATATAACGACTGCACCTTTGCCGGTACACGAC                      <  SRR19973083.2402775/150‑1 (MQ=60)
                                                                                                                               GATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTT                    >  SRR19973083.4645866/1‑150 (MQ=60)
                                                                                                                               GATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTT                    >  SRR19973083.4834654/1‑150 (MQ=60)
                                                                                                                                  CGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTC                 >  SRR19973083.4151083/1‑150 (MQ=60)
                                                                                                                                  CGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTC                 >  SRR19973083.4150967/1‑150 (MQ=60)
                                                                                                                                  CGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTC                 <  SRR19973083.4726326/150‑1 (MQ=60)
                                                                                                                                  CGGGCCTTGCGCCAGTTGCTCTGCTAACGCTGCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCATGTTACTGCTTGCCATCAAAATCTCCTTTTATATAACGACTGCACCTTTGCCGGTACACGACTTTTC                 <  SRR19973083.3523553/150‑1 (MQ=60)
                                                                                                                                      CCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCA             <  SRR19973083.3271836/150‑1 (MQ=60)
                                                                                                                                       CTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAG            >  SRR19973083.3196733/1‑150 (MQ=60)
                                                                                                                                       CTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAG            >  SRR19973083.3196968/1‑150 (MQ=60)
                                                                                                                                       CTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAG            >  SRR19973083.4929798/1‑150 (MQ=60)
                                                                                                                                       CTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAG            >  SRR19973083.4998602/1‑150 (MQ=60)
                                                                                                                                       CTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAG            >  SRR19973083.4999040/1‑150 (MQ=60)
                                                                                                                                        TTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGA           >  SRR19973083.3297804/1‑150 (MQ=60)
                                                                                                                                        TTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGA           >  SRR19973083.4432235/1‑150 (MQ=60)
                                                                                                                                         TGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGAT          >  SRR19973083.3857481/1‑150 (MQ=60)
                                                                                                                                           CGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGC        >  SRR19973083.911012/1‑150 (MQ=60)
                                                                                                                                            GCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTCTATAACGACTGCACCGTTGCCGGTACACGACTTTTCGCCAGATGCG       >  SRR19973083.564498/1‑150 (MQ=60)
                                                                                                                                            GCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCG       >  SRR19973083.564576/1‑150 (MQ=60)
                                                                                                                                            GCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCG       >  SRR19973083.4262255/1‑150 (MQ=60)
                                                                                                                                            GCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCG       >  SRR19973083.4262290/1‑150 (MQ=60)
                                                                                                                                            GCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGAAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTATTCGACAGATGCG       >  SRR19973083.4262321/1‑150 (MQ=60)
                                                                                                                                             CCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTTTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGA      <  SRR19973083.3954866/150‑1 (MQ=60)
                                                                                                                                             CCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCGGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGA      <  SRR19973083.3955268/150‑1 (MQ=60)
                                                                                                                                              CAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCAATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAA     <  SRR19973083.5134928/150‑1 (MQ=60)
                                                                                                                                              CAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGAAATTGTTTAATAAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAA     <  SRR19973083.4868798/150‑1 (MQ=60)
                                                                                                                                               AGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAA    <  SRR19973083.3887981/150‑1 (MQ=60)
                                                                                                                                                 TTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGA  <  SRR19973083.301213/150‑1 (MQ=60)
                                                                                                                                                  |                                                                                                                                                    
CCGTCGCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGA  >  CP009273/1711251‑1711545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑