Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,711,397 C→T 100% R28Q (CGA→CAA)  tyrS ← tyrosyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,711,3970CT99.6% 880.2 / ‑4.5 244R28Q (CGA→CAA) tyrStyrosyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (120/123);  total (120/124)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 244 total aligned reads displayed.

CCGTCGCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAA  >  CP009273/1711251‑1711543
                                                                                                                                                  |                                                                                                                                                  
 cGTCGCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGctc                                                                                                                                                <  2:2942604/150‑1 (MQ=255)
  gTCGCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGctct                                                                                                                                               >  2:3546556/1‑150 (MQ=255)
    cgcgCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGc                                                                                                                                             >  2:1790164/1‑150 (MQ=255)
      cgccgcCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTa                                                                                                                                           <  1:2570335/150‑1 (MQ=255)
      cgccgcCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGATGCTCTGCTa                                                                                                                                           >  2:3286165/1‑150 (MQ=255)
        ccgccTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAAc                                                                                                                                         <  2:1966278/150‑1 (MQ=255)
         cgccTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACg                                                                                                                                        >  2:4015673/1‑150 (MQ=255)
         cgccTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACg                                                                                                                                        >  2:19609/1‑150 (MQ=255)
          gccTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGc                                                                                                                                       >  2:4141308/1‑150 (MQ=255)
               ccAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAAGAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCt                                                                                                                                  <  2:1403633/150‑1 (MQ=255)
                    gcAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTcgtcc                                                                                                                             <  2:1065913/150‑1 (MQ=255)
                     cAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTcgtccg                                                                                                                            <  2:587309/150‑1 (MQ=255)
                      aaCCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTCGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTcgtccgt                                                                                                                           >  2:3803812/1‑150 (MQ=255)
                        ccGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCa                                                                                                                         <  1:4013625/150‑1 (MQ=255)
                            cTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTg                                                                                                                     <  1:362553/150‑1 (MQ=255)
                               gtgGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGc                                                                                                                  <  1:3415639/150‑1 (MQ=255)
                                tgGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCt                                                                                                                 >  1:3950314/1‑150 (MQ=255)
                                tgGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCt                                                                                                                 >  1:330236/1‑150 (MQ=255)
                                tgGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCt                                                                                                                 <  1:757710/150‑1 (MQ=255)
                                 ggCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTa                                                                                                                >  2:2855007/1‑150 (MQ=255)
                                   cccGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCATCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTAcc                                                                                                              <  2:2551949/150‑1 (MQ=255)
                                   cccGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTAcc                                                                                                              >  1:4112647/1‑150 (MQ=255)
                                    ccGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCa                                                                                                             >  1:3559193/1‑150 (MQ=255)
                                            tgGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGc                                                                                                     >  2:2629395/1‑150 (MQ=255)
                                            tgGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGc                                                                                                     >  1:1407423/1‑150 (MQ=255)
                                             ggAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGAGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGct                                                                                                    >  1:3454333/1‑150 (MQ=255)
                                                aGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCtt                                                                                                 <  2:3661374/150‑1 (MQ=255)
                                                 gCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTg                                                                                                <  1:2174677/150‑1 (MQ=255)
                                                 gCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTg                                                                                                <  1:1511007/150‑1 (MQ=255)
                                                 gCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTg                                                                                                <  1:1742108/150‑1 (MQ=255)
                                                   gTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCa                                                                                              >  1:3999232/1‑150 (MQ=255)
                                                   gTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCa                                                                                              <  2:3267856/150‑1 (MQ=255)
                                                      tCAGGCATAACAATGGAACATGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCaagt                                                                                           <  1:1081392/150‑3 (MQ=255)
                                                      tCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAAtt                                                                                           >  2:4089136/1‑150 (MQ=255)
                                                       cAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTg                                                                                          <  1:891597/150‑1 (MQ=255)
                                                        aGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGt                                                                                         <  2:3051137/150‑1 (MQ=255)
                                                          gCATAAAAATGGAACAAGATGCCCCAAATGCAAGCTGTAAGCGTTAGGATCGAAGCCGCAATAGAGCGCGATCTGGCCTTGCGCCAGTTGCTCTGCTAACGCTTACTCGTCCGTCACCTGGGCTAAAAGCCCACGCTCTTGCAATTGttt                                                                                       <  2:1924276/150‑1 (MQ=255)
                                                             tAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAAt                                                                                    <  2:1327742/150‑1 (MQ=255)
                                                              aaCAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGAGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATc                                                                                   >  1:1051212/1‑150 (MQ=255)
                                                               aCAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCa                                                                                  >  1:1461986/1‑150 (MQ=255)
                                                                cAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCaa                                                                                 >  1:868988/1‑150 (MQ=255)
                                                                 aaTGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAg                                                                                <  2:555279/150‑1 (MQ=255)
                                                                   tGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGtt                                                                              >  1:2888951/1‑150 (MQ=255)
                                                                       aCAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTg                                                                          <  2:642200/150‑1 (MQ=255)
                                                                         aaGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCt                                                                        <  2:46774/150‑1 (MQ=255)
                                                                         aaGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCt                                                                        <  1:189845/150‑1 (MQ=255)
                                                                          aGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCtt                                                                       <  2:3761623/150‑1 (MQ=255)
                                                                           gATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTg                                                                      >  1:3713074/1‑150 (MQ=255)
                                                                           gATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTg                                                                      <  1:2685519/150‑1 (MQ=255)
                                                                             tGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGcc                                                                    >  1:2934199/1‑150 (MQ=255)
                                                                              gCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCa                                                                   <  2:3584367/150‑1 (MQ=255)
                                                                               ccccAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCACTTGTTTAATCAAGTTACTGCTTGCCAt                                                                  >  2:2821051/1‑150 (MQ=255)
                                                                                cccAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATc                                                                 >  2:276933/1‑150 (MQ=255)
                                                                                cccAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATc                                                                 <  2:2702099/150‑1 (MQ=255)
                                                                                cccAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATc                                                                 >  2:239899/1‑150 (MQ=255)
                                                                                cccAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATc                                                                 >  1:3742819/1‑150 (MQ=255)
                                                                                  cAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCaa                                                               <  1:124992/150‑1 (MQ=255)
                                                                                    aaTGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCaaaa                                                             <  1:1954029/150‑1 (MQ=255)
                                                                                    aaTGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCaaaa                                                             <  1:2722370/150‑1 (MQ=255)
                                                                                     aTGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAAt                                                            >  1:2943803/1‑150 (MQ=255)
                                                                                      tGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAAtc                                                           >  2:1844220/1‑150 (MQ=255)
                                                                                      tGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAAtc                                                           <  1:714243/150‑1 (MQ=255)
                                                                                      tGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAAtc                                                           >  1:3323400/1‑150 (MQ=255)
                                                                                         aaGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTcc                                                        >  2:1478892/1‑150 (MQ=255)
                                                                                           gCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCAt                                                      <  1:1687177/150‑1 (MQ=255)
                                                                                           gCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCAt                                                      <  2:2248116/150‑1 (MQ=255)
                                                                                            cTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATg                                                     >  1:2792272/1‑150 (MQ=255)
                                                                                              gTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGta                                                   >  2:1743413/1‑150 (MQ=255)
                                                                                               tCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGtat                                                  >  2:3696195/1‑150 (MQ=255)
                                                                                               tCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGtat                                                  <  2:3020827/150‑1 (MQ=255)
                                                                                                cAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGtata                                                 >  1:3156514/1‑150 (MQ=255)
                                                                                                cAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGtata                                                 <  1:2238399/150‑1 (MQ=255)
                                                                                                    ggTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAAc                                             <  1:3367657/150‑1 (MQ=255)
                                                                                                    ggTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAAc                                             >  1:986451/1‑150 (MQ=255)
                                                                                                     gTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACg                                            <  1:2671981/150‑1 (MQ=255)
                                                                                                     gTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACg                                            <  1:2407890/150‑1 (MQ=255)
                                                                                                       aGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGAc                                          >  2:3456198/1‑150 (MQ=255)
                                                                                                        ggATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACt                                         <  2:502884/150‑1 (MQ=255)
                                                                                                        ggATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACt                                         <  2:315545/150‑1 (MQ=255)
                                                                                                           tCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCa                                      <  2:689727/150‑1 (MQ=255)
                                                                                                           tCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCa                                      <  2:1363322/150‑1 (MQ=255)
                                                                                                            cGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCAc                                     >  1:82938/1‑150 (MQ=255)
                                                                                                              aaGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCt                                   >  2:244875/1‑150 (MQ=255)
                                                                                                              aaGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCt                                   <  1:3174368/150‑1 (MQ=255)
                                                                                                               aGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCtt                                  <  1:2991128/150‑1 (MQ=255)
                                                                                                                 ccGCATTAGAGCGGGATCGGGCCTTGCGCCATTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGAAATTGTTTAATCATGTTACTGCTTGCAATCAAAATATCAATGTATATAACGACTGCACCTTTg                                <  1:3291733/150‑1 (MQ=255)
                                                                                                                  cGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGc                               >  2:2183441/1‑150 (MQ=255)
                                                                                                                     aaTAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCgg                            >  1:1800788/1‑150 (MQ=255)
                                                                                                                           gcgcGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACg                      <  1:2080426/150‑1 (MQ=255)
                                                                                                                              cgATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACt                   >  1:2046228/1‑150 (MQ=255)
                                                                                                                               gATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACtt                  >  2:2164857/1‑150 (MQ=255)
                                                                                                                                aTCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACttt                 <  1:2286994/150‑1 (MQ=255)
                                                                                                                                aTCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACttt                 >  2:2612444/1‑150 (MQ=255)
                                                                                                                                 tCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACtttt                >  1:2449674/1‑150 (MQ=255)
                                                                                                                                  cGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTc               >  2:1789806/1‑150 (MQ=255)
                                                                                                                                      ccTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCa           >  1:2303779/1‑150 (MQ=255)
                                                                                                                                       cTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAg          >  2:2375968/1‑150 (MQ=255)
                                                                                                                                         tGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGAt        >  2:2136365/1‑150 (MQ=255)
                                                                                                                                              cAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGaa   <  1:1004207/150‑1 (MQ=255)
                                                                                                                                               aGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGaaa  <  1:4117160/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CCGTCGCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAA  >  CP009273/1711251‑1711543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCGTCGCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAA  >  CP009273/1711251‑1711543
                                                                                                                                                  |                                                                                                                                                  
CCGTCGCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCT                                                                                                                                                 <  SRR19973074.2712834/150‑1 (MQ=60)
  GTCGCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCT                                                                                                                                               <  SRR19973074.1581904/150‑1 (MQ=60)
  GTCGCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCT                                                                                                                                               <  SRR19973074.3741806/150‑1 (MQ=60)
   TCGCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTG                                                                                                                                              <  SRR19973074.3845413/150‑1 (MQ=60)
      CGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTA                                                                                                                                           <  SRR19973074.2575134/150‑1 (MQ=60)
      CGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTA                                                                                                                                           <  SRR19973074.2575046/150‑1 (MQ=60)
              ACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCC                                                                                                                                   >  SRR19973074.4153174/1‑150 (MQ=60)
                CAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTC                                                                                                                                 >  SRR19973074.671621/1‑150 (MQ=60)
                CAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTC                                                                                                                                 <  SRR19973074.3985961/150‑1 (MQ=60)
                 AGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCG                                                                                                                                >  SRR19973074.35581/1‑150 (MQ=60)
                 AGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCG                                                                                                                                >  SRR19973074.35622/1‑150 (MQ=60)
                    GCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCC                                                                                                                             >  SRR19973074.2422574/1‑150 (MQ=60)
                        CCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCA                                                                                                                         <  SRR19973074.4021049/150‑1 (MQ=60)
                        CCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCA                                                                                                                         <  SRR19973074.1936213/150‑1 (MQ=60)
                        CCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCA                                                                                                                         <  SRR19973074.1862428/150‑1 (MQ=60)
                         CGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCATCGTCCGTCAC                                                                                                                        >  SRR19973074.868906/1‑150 (MQ=60)
                          GGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACC                                                                                                                       <  SRR19973074.1833066/150‑1 (MQ=60)
                            CTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTG                                                                                                                     <  SRR19973074.363217/150‑1 (MQ=60)
                              TGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGG                                                                                                                   <  SRR19973074.4102200/150‑1 (MQ=60)
                               GTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGC                                                                                                                  <  SRR19973074.3421946/150‑1 (MQ=60)
                                TGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCT                                                                                                                 <  SRR19973074.759152/150‑1 (MQ=60)
                                TGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCT                                                                                                                 <  SRR19973074.4031672/150‑1 (MQ=60)
                                TGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCT                                                                                                                 >  SRR19973074.3957592/1‑150 (MQ=60)
                                TGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCT                                                                                                                 <  SRR19973074.2668236/150‑1 (MQ=60)
                                TGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCT                                                                                                                 >  SRR19973074.3957370/1‑150 (MQ=60)
                                TGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCT                                                                                                                 >  SRR19973074.330863/1‑150 (MQ=60)
                                TGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCT                                                                                                                 >  SRR19973074.330838/1‑150 (MQ=60)
                                TGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCT                                                                                                                 >  SRR19973074.1156513/1‑150 (MQ=60)
                                  GCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTAC                                                                                                               >  SRR19973074.3118548/1‑150 (MQ=60)
                                   CCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACC                                                                                                              >  SRR19973074.4120240/1‑150 (MQ=60)
                                    CCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCA                                                                                                             >  SRR19973074.3565753/1‑150 (MQ=60)
                                    CCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCA                                                                                                             >  SRR19973074.3565752/1‑150 (MQ=60)
                                    CCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCA                                                                                                             <  SRR19973074.2764176/150‑1 (MQ=60)
                                      GCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGC                                                                                                           >  SRR19973074.731587/1‑150 (MQ=60)
                                       CCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCC                                                                                                          <  SRR19973074.1973654/150‑1 (MQ=60)
                                          GCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCC                                                                                                       <  SRR19973074.366251/150‑1 (MQ=60)
                                            TGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGC                                                                                                     >  SRR19973074.1410016/1‑150 (MQ=60)
                                            TGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGC                                                                                                     >  SRR19973074.1453455/1‑150 (MQ=60)
                                             GGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCT                                                                                                    <  SRR19973074.1981758/150‑1 (MQ=60)
                                             GGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGAGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCT                                                                                                    >  SRR19973074.3460686/1‑150 (MQ=60)
                                                 GCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTG                                                                                                <  SRR19973074.1513856/150‑1 (MQ=60)
                                                 GCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTG                                                                                                <  SRR19973074.1513920/150‑1 (MQ=60)
                                                 GCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTG                                                                                                <  SRR19973074.2178762/150‑1 (MQ=60)
                                                 GCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTG                                                                                                <  SRR19973074.2178692/150‑1 (MQ=60)
                                                 GCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTG                                                                                                <  SRR19973074.1745339/150‑1 (MQ=60)
                                                  CGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGC                                                                                               >  SRR19973074.2218277/1‑150 (MQ=60)
                                                   GTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCA                                                                                              >  SRR19973074.4006620/1‑150 (MQ=60)
                                                   GTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCA                                                                                              >  SRR19973074.4006644/1‑150 (MQ=60)
                                                   GTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCA                                                                                              >  SRR19973074.763224/1‑150 (MQ=60)
                                                      TCAGGCATAACAATGGAACATGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAAGT                                                                                           <  SRR19973074.1083413/150‑1 (MQ=60)
                                                       CAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTG                                                                                          <  SRR19973074.3302615/150‑1 (MQ=60)
                                                       CAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTG                                                                                          <  SRR19973074.3302844/150‑1 (MQ=60)
                                                       CAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTG                                                                                          <  SRR19973074.893302/150‑1 (MQ=60)
                                                       CAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTG                                                                                          <  SRR19973074.764781/150‑1 (MQ=60)
                                                         GGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTT                                                                                        >  SRR19973074.2864490/1‑150 (MQ=60)
                                                              AACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGAGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATC                                                                                   >  SRR19973074.1053147/1‑150 (MQ=60)
                                                              AACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGAGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATC                                                                                   >  SRR19973074.1053805/1‑150 (MQ=60)
                                                               ACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCA                                                                                  >  SRR19973074.3336409/1‑150 (MQ=60)
                                                               ACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCA                                                                                  >  SRR19973074.1464723/1‑150 (MQ=60)
                                                               ACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCA                                                                                  >  SRR19973074.1775174/1‑150 (MQ=60)
                                                                CAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTCACGCTTCCTCGTCCGGCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAA                                                                                 >  SRR19973074.2963349/1‑150 (MQ=60)
                                                                CAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAA                                                                                 >  SRR19973074.870663/1‑150 (MQ=60)
                                                                   TGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTT                                                                              <  SRR19973074.2618904/150‑1 (MQ=60)
                                                                   TGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTT                                                                              >  SRR19973074.3397333/1‑150 (MQ=60)
                                                                   TGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTT                                                                              <  SRR19973074.1306041/150‑1 (MQ=60)
                                                                   TGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTT                                                                              >  SRR19973074.2894309/1‑150 (MQ=60)
                                                                    GGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTA                                                                             >  SRR19973074.3432614/1‑150 (MQ=60)
                                                                      AACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACT                                                                           >  SRR19973074.2417768/1‑150 (MQ=60)
                                                                         AAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCT                                                                        <  SRR19973074.190249/150‑1 (MQ=60)
                                                                         AAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCT                                                                        >  SRR19973074.151146/1‑150 (MQ=60)
                                                                           GATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTG                                                                      >  SRR19973074.1697606/1‑150 (MQ=60)
                                                                           GATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTG                                                                      >  SRR19973074.2088536/1‑150 (MQ=60)
                                                                           GATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTG                                                                      >  SRR19973074.3719933/1‑150 (MQ=60)
                                                                           GATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTG                                                                      <  SRR19973074.2339451/150‑1 (MQ=60)
                                                                           GATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTG                                                                      <  SRR19973074.2690509/150‑1 (MQ=60)
                                                                             TGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCC                                                                    >  SRR19973074.2939655/1‑150 (MQ=60)
                                                                             TGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCC                                                                    >  SRR19973074.2939603/1‑150 (MQ=60)
                                                                                CCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATC                                                                 >  SRR19973074.3749706/1‑150 (MQ=60)
                                                                                  CAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAA                                                               <  SRR19973074.3177547/150‑1 (MQ=60)
                                                                                  CAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAA                                                               <  SRR19973074.125281/150‑1 (MQ=60)
                                                                                   AAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAA                                                              >  SRR19973074.1018473/1‑150 (MQ=60)
                                                                                    AATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAA                                                             <  SRR19973074.2727352/150‑1 (MQ=60)
                                                                                    AATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAA                                                             <  SRR19973074.2727403/150‑1 (MQ=60)
                                                                                    AATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAA                                                             <  SRR19973074.1957761/150‑1 (MQ=60)
                                                                                     ATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAAT                                                            >  SRR19973074.2949239/1‑150 (MQ=60)
                                                                                     ATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAAT                                                            >  SRR19973074.2949274/1‑150 (MQ=60)
                                                                                      TGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATC                                                           >  SRR19973074.3329576/1‑150 (MQ=60)
                                                                                      TGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATC                                                           <  SRR19973074.715574/150‑1 (MQ=60)
                                                                                           GCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCAT                                                      <  SRR19973074.1690333/150‑1 (MQ=60)
                                                                                            CTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATG                                                     >  SRR19973074.2797411/1‑150 (MQ=60)
                                                                                              GTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTA                                                   <  SRR19973074.873998/150‑1 (MQ=60)
                                                                                              GTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTA                                                   <  SRR19973074.874007/150‑1 (MQ=60)
                                                                                                CAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATA                                                 >  SRR19973074.3162316/1‑150 (MQ=60)
                                                                                                CAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATA                                                 >  SRR19973074.3162359/1‑150 (MQ=60)
                                                                                                CAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATA                                                 >  SRR19973074.3407320/1‑150 (MQ=60)
                                                                                                CAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATA                                                 <  SRR19973074.2242607/150‑1 (MQ=60)
                                                                                                CAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATA                                                 <  SRR19973074.2242611/150‑1 (MQ=60)
                                                                                                  GCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATA                                               <  SRR19973074.3190227/150‑1 (MQ=60)
                                                                                                    GGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAAC                                             <  SRR19973074.3373900/150‑1 (MQ=60)
                                                                                                    GGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAAC                                             >  SRR19973074.988297/1‑150 (MQ=60)
                                                                                                     GTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACG                                            <  SRR19973074.2412350/150‑1 (MQ=60)
                                                                                                     GTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACG                                            <  SRR19973074.2676956/150‑1 (MQ=60)
                                                                                                     GTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACG                                            <  SRR19973074.2677073/150‑1 (MQ=60)
                                                                                                          ATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGC                                       >  SRR19973074.2246366/1‑150 (MQ=60)
                                                                                                           TCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCA                                      >  SRR19973074.2301215/1‑150 (MQ=60)
                                                                                                            CGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCAC                                     >  SRR19973074.83139/1‑150 (MQ=60)
                                                                                                              AAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCT                                   <  SRR19973074.3180210/150‑1 (MQ=60)
                                                                                                              AAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCT                                   <  SRR19973074.1219658/150‑1 (MQ=60)
                                                                                                               AGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTT                                  <  SRR19973074.2996688/150‑1 (MQ=60)
                                                                                                                 CCGCATTAGAGCGGGATCGGGCCTTGCGCCATTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGAAATTGTTTAATCATGTTACTGCTTGCAATCAAAATATCAATGTATATAACGACTGCACCTTTG                                <  SRR19973074.3297842/150‑1 (MQ=60)
                                                                                                                  CGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGC                               >  SRR19973074.1419434/1‑150 (MQ=60)
                                                                                                                     AATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGG                            >  SRR19973074.1804211/1‑150 (MQ=60)
                                                                                                                        AGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTAC                         >  SRR19973074.2968511/1‑150 (MQ=60)
                                                                                                                           GCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACG                      <  SRR19973074.2084318/150‑1 (MQ=60)
                                                                                                                              CGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACT                   >  SRR19973074.2050337/1‑150 (MQ=60)
                                                                                                                              CGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACT                   >  SRR19973074.2050079/1‑150 (MQ=60)
                                                                                                                                ATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTT                 <  SRR19973074.2291280/150‑1 (MQ=60)
                                                                                                                                 TCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTT                >  SRR19973074.2454208/1‑150 (MQ=60)
                                                                                                                                   GGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCG              <  SRR19973074.2892292/150‑1 (MQ=60)
                                                                                                                                      CCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCA           >  SRR19973074.2308085/1‑150 (MQ=60)
                                                                                                                                          GCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATG       <  SRR19973074.3711007/150‑1 (MQ=60)
                                                                                                                                            GCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCG     <  SRR19973074.929824/150‑1 (MQ=60)
                                                                                                                                              CAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAA   <  SRR19973074.1006071/150‑1 (MQ=60)
                                                                                                                                               AGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAA  <  SRR19973074.4124758/150‑1 (MQ=60)
                                                                                                                                               AGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAA  <  SRR19973074.4124645/150‑1 (MQ=60)
                                                                                                                                                  |                                                                                                                                                  
CCGTCGCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAA  >  CP009273/1711251‑1711543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑