Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,711,397 C→T 100% R28Q (CGA→CAA)  tyrS ← tyrosyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,711,3970CT99.6% 800.6 / ‑4.7 240R28Q (CGA→CAA) tyrStyrosyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (136/103);  minor base A (1/0);  total (137/103)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 240 total aligned reads displayed.

CGTCGCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGA  >  CP009273/1711252‑1711541
                                                                                                                                                 |                                                                                                                                                
 gTCGCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGct                                                                                                                                               <  1:3481089/148‑1 (MQ=255)
  tCGCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGctc                                                                                                                                              <  1:2950982/148‑1 (MQ=255)
   cgcgCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGctct                                                                                                                                             >  1:2453921/1‑148 (MQ=255)
       ccgccTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTa                                                                                                                                         <  1:2217144/148‑1 (MQ=255)
           cgaCCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGc                                                                                                                                     <  1:3512209/146‑1 (MQ=255)
             aCCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTACCGCtt                                                                                                                                   >  2:1587777/1‑148 (MQ=255)
               cAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTcc                                                                                                                                 <  2:1422129/148‑1 (MQ=255)
                  cgcAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTcg                                                                                                                              >  2:1863338/1‑148 (MQ=255)
                    cAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTcgtc                                                                                                                            <  1:3040837/148‑1 (MQ=255)
                         ggCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCa                                                                                                                       >  2:806311/1‑148 (MQ=255)
                           cTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCAcc                                                                                                                     >  2:3540648/1‑148 (MQ=255)
                           cTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCAcc                                                                                                                     <  1:124007/148‑1 (MQ=255)
                           cTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCAcc                                                                                                                     >  1:2786500/1‑148 (MQ=255)
                             tgtgGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTg                                                                                                                   >  2:867424/1‑148 (MQ=255)
                                 gCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGAGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGACACCTGGGCt                                                                                                               >  1:1990554/1‑148 (MQ=255)
                                    cGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTAcc                                                                                                            >  2:3049607/1‑148 (MQ=255)
                                    cGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTAcc                                                                                                            >  1:2728763/1‑148 (MQ=255)
                                        tgctgGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTAAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGcc                                                                                                        >  2:2625803/1‑148 (MQ=255)
                                               aGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGctc                                                                                                 >  1:3404287/1‑148 (MQ=255)
                                               aGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGACAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGctc                                                                                                 >  1:1498803/1‑148 (MQ=255)
                                                gCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGctct                                                                                                >  2:3537803/1‑148 (MQ=255)
                                                    ttCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCa                                                                                            >  2:372702/1‑148 (MQ=255)
                                                    ttCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCa                                                                                            <  1:3760047/148‑1 (MQ=255)
                                                     tCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCaa                                                                                           <  2:3013262/148‑1 (MQ=255)
                                                      cAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAAt                                                                                          <  2:2862109/148‑1 (MQ=255)
                                                      cAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAAt                                                                                          <  2:3294173/148‑1 (MQ=255)
                                                       aGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAAtt                                                                                         >  1:1015313/1‑148 (MQ=255)
                                                        ggCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTg                                                                                        >  1:3260935/1‑148 (MQ=255)
                                                        ggCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTg                                                                                        <  2:2807879/148‑1 (MQ=255)
                                                        ggCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTg                                                                                        <  2:3775335/148‑1 (MQ=255)
                                                         gCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGt                                                                                       <  2:97239/148‑1 (MQ=255)
                                                         gCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGt                                                                                       <  1:1801233/148‑1 (MQ=255)
                                                              aCAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAAt                                                                                  <  2:1028169/148‑1 (MQ=255)
                                                              aCAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTGGCAATTGTTTAAt                                                                                  >  2:3654884/1‑148 (MQ=255)
                                                               cAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATc                                                                                 >  1:3086503/1‑148 (MQ=255)
                                                               cAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATc                                                                                 >  1:63040/1‑148 (MQ=255)
                                                                   ggAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGt                                                                             <  1:232338/148‑1 (MQ=255)
                                                                    gAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGtt                                                                            >  1:956039/1‑148 (MQ=255)
                                                                     aaCAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTa                                                                           <  1:2056166/148‑1 (MQ=255)
                                                                      aCAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTAc                                                                          >  1:2826399/1‑148 (MQ=255)
                                                                       cAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGNTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACt                                                                         >  1:1936781/1‑148 (MQ=255)
                                                                         aGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGc                                                                       <  1:3641476/148‑1 (MQ=255)
                                                                          gATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCt                                                                      >  2:2697235/1‑148 (MQ=255)
                                                                          gATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCt                                                                      >  2:1529806/1‑148 (MQ=255)
                                                                           aTGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCtt                                                                     >  2:2052900/1‑148 (MQ=255)
                                                                             gCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGc                                                                   <  1:42515/148‑1 (MQ=255)
                                                                              ccccAAATGCAAGCTGTCAGCGTTAGGATCGTAGCAGCTTTAGAGCGCGATCGGGCCTTGCGCCAGTTTCTCTGCTAACGCTTCCTAGTCCGTCACCTGGTCTACCAGCCCCCTCTCTTGCAATTGTTTAATAAAGTTACTGCTTGcc                                                                  <  2:3138340/148‑1 (MQ=255)
                                                                                  aaaTGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCa                                                              >  1:2638751/1‑148 (MQ=255)
                                                                                   aaTGCACGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAAGTGTTTAATCAAGTTACTGCTTGCCATCaa                                                             <  2:3590460/148‑1 (MQ=255)
                                                                                   aaTGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCaa                                                             >  2:1468655/1‑148 (MQ=255)
                                                                                   aaTGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCaa                                                             <  2:1696986/148‑1 (MQ=255)
                                                                                    aTGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCaaa                                                            <  2:2007580/148‑1 (MQ=255)
                                                                                      gCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAAt                                                          <  2:3500779/148‑1 (MQ=255)
                                                                                      gCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAAt                                                          <  1:372702/148‑1 (MQ=255)
                                                                                      gCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAAt                                                          <  2:1602935/148‑1 (MQ=255)
                                                                                         aGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAAtctc                                                       >  2:1158404/1‑148 (MQ=255)
                                                                                         aGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAAtctc                                                       >  1:164416/1‑148 (MQ=255)
                                                                                         aGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAAtctc                                                       >  1:3624351/1‑148 (MQ=255)
                                                                                         aGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAAtctc                                                       >  2:636379/1‑148 (MQ=255)
                                                                                          gCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTcc                                                      >  2:1846886/1‑148 (MQ=255)
                                                                                          gCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTcc                                                      >  2:2246578/1‑148 (MQ=255)
                                                                                          gCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTcc                                                      >  2:3391731/1‑148 (MQ=255)
                                                                                          gCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTcc                                                      >  1:2865953/1‑148 (MQ=255)
                                                                                           cTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCa                                                     <  2:3454755/148‑1 (MQ=255)
                                                                                           cTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCa                                                     <  2:1095250/148‑1 (MQ=255)
                                                                                           cTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCa                                                     <  2:428215/148‑1 (MQ=255)
                                                                                           cTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCa                                                     <  1:1108138/148‑1 (MQ=255)
                                                                                               cAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGta                                                 >  1:2244867/1‑148 (MQ=255)
                                                                                                aGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGtat                                                <  1:1001836/148‑1 (MQ=255)
                                                                                                aGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGtat                                                <  1:2481487/148‑1 (MQ=255)
                                                                                                 gCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGtata                                               >  2:2276697/1‑148 (MQ=255)
                                                                                                  cGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGtatat                                              <  2:133779/148‑1 (MQ=255)
                                                                                                  cGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGtatat                                              >  2:714262/1‑148 (MQ=255)
                                                                                                   ggTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGtatata                                             <  1:1545573/148‑1 (MQ=255)
                                                                                                     tAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAAc                                           >  1:1708077/1‑148 (MQ=255)
                                                                                                     tAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAAc                                           >  1:54156/1‑148 (MQ=255)
                                                                                                     tAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAAc                                           >  1:1554972/1‑148 (MQ=255)
                                                                                                     aaGGATCTAATCCGAAATAGAGCCCGAACGGGCATAGCGCCAGTTGCTCTGCTAACTCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTCCTAGCCATCAAAATCTCCATGTAAATAAc                                           >  2:1894564/2‑148 (MQ=255)
                                                                                                        gATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGAc                                        >  2:1253208/1‑148 (MQ=255)
                                                                                                          tCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTg                                      >  2:2946083/1‑148 (MQ=255)
                                                                                                          tCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTg                                      >  2:2942695/1‑148 (MQ=255)
                                                                                                            gAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCa                                    >  1:2029768/1‑148 (MQ=255)
                                                                                                              aGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCAcc                                  >  2:1820505/1‑148 (MQ=255)
                                                                                                              aGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTAATGCTTGCCATCAAAATCTCCATGTATATAACGACTGCAcc                                  >  1:3192181/1‑148 (MQ=255)
                                                                                                               gCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCt                                 >  1:2401354/1‑148 (MQ=255)
                                                                                                               gCCGCAATAGAGCGCGATAGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCt                                 >  2:2078664/1‑148 (MQ=255)
                                                                                                                      tAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCgg                          >  1:1768309/1‑148 (MQ=255)
                                                                                                                        gagCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTa                        >  1:468599/1‑148 (MQ=255)
                                                                                                                         agCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTac                       >  1:3288500/1‑148 (MQ=255)
                                                                                                                          gcgcGTTCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCGGTCACCTGGGCTACCAGCCCCCGCTATTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCAATGTATAGAACGACTGCACCTTTGCCGGTaca                      <  2:3157845/148‑1 (MQ=255)
                                                                                                                                tCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCAATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACtt                <  1:3072526/148‑1 (MQ=255)
                                                                                                                                gcGGGCCTGGCGCCTGTTGCTCTGATACCGCTTCCGCGTCCGTCACCTGGGCTACCCGCCCCCGCTCTTGAATTTTTTTAATCATTTTACTGCTTGCCATAAAAATCTCCTTGTATATAACGACTGCACCTTTGCCGGTACACGACtt                <  1:2623028/147‑1 (MQ=255)
                                                                                                                                 cGGGCGTTGCGCCTGTTGCTCTGATAACGGTTCCTCGTCCGTCACGTGGGCTACCAGCCCCCGCTCTTGCATTTTTTTAATCAATTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACttt               <  1:806311/148‑1 (MQ=255)
                                                                                                                                  gggCCTTGCGCCTGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGAAATTGTTTAATCAATTTACTGCTTGCCATAAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACtttt              <  1:829344/148‑1 (MQ=255)
                                                                                                                                   ggCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTc             >  2:914736/1‑148 (MQ=255)
                                                                                                                                        tGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAg        <  1:1158404/148‑1 (MQ=255)
                                                                                                                                             cAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCg   >  2:455474/1‑148 (MQ=255)
                                                                                                                                              aGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGa  <  2:3404287/148‑1 (MQ=255)
                                                                                                                                              aGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGa  >  2:953218/1‑148 (MQ=255)
                                                                                                                                              aGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGa  <  2:1498803/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CGTCGCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGA  >  CP009273/1711252‑1711541

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCCGTCGCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAG  >  CP009273/1711250‑1711546
                                                                                                                                                   |                                                                                                                                                     
CCCGTCGCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGC                                                                                                                                                     >  SRR19973069.968605/1‑150 (MQ=60)
CCCGTCGCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGC                                                                                                                                                     >  SRR19973069.2893449/1‑150 (MQ=60)
 CCGTCGCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCT                                                                                                                                                    <  SRR19973069.3077280/150‑1 (MQ=60)
 CCGTCGCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCT                                                                                                                                                    >  SRR19973069.3026058/1‑150 (MQ=60)
   GTCGCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCT                                                                                                                                                  <  SRR19973069.3654524/150‑1 (MQ=60)
   GTCGCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCT                                                                                                                                                  <  SRR19973069.3503573/150‑1 (MQ=60)
   GTCGCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCT                                                                                                                                                  >  SRR19973069.2470422/1‑150 (MQ=60)
    TCGCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTG                                                                                                                                                 <  SRR19973069.1768184/150‑1 (MQ=60)
    TCGCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTG                                                                                                                                                 <  SRR19973069.1539777/150‑1 (MQ=60)
    TCGCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTG                                                                                                                                                 <  SRR19973069.2970564/150‑1 (MQ=60)
      GCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACACTGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCT                                                                                                                                               <  SRR19973069.1356983/150‑1 (MQ=60)
      GCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCT                                                                                                                                               <  SRR19973069.2468759/150‑1 (MQ=60)
         CCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAAC                                                                                                                                            <  SRR19973069.2232151/150‑1 (MQ=60)
             CGACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTT                                                                                                                                        <  SRR19973069.3534847/150‑1 (MQ=60)
                 CAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTC                                                                                                                                    >  SRR19973069.2570039/1‑150 (MQ=60)
                      CAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCG                                                                                                                               <  SRR19973069.3060987/150‑1 (MQ=60)
                           GGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACC                                                                                                                          >  SRR19973069.2805040/1‑150 (MQ=60)
                             CTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTG                                                                                                                        <  SRR19973069.125180/150‑1 (MQ=60)
                                 TGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGAGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGACACCTGGGCT                                                                                                                    >  SRR19973069.2004429/1‑150 (MQ=60)
                                    CNCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACC                                                                                                                 >  SRR19973069.581000/1‑150 (MQ=60)
                                    CCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACC                                                                                                                 >  SRR19973069.2747037/1‑150 (MQ=60)
                                     CCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCA                                                                                                                >  SRR19973069.1145709/1‑150 (MQ=60)
                                             TGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGC                                                                                                        >  SRR19973069.3455799/1‑150 (MQ=60)
                                               GAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTC                                                                                                      >  SRR19973069.3422941/1‑150 (MQ=60)
                                               GAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTC                                                                                                      >  SRR19973069.3426492/1‑150 (MQ=60)
                                               GAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGACAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTC                                                                                                      >  SRR19973069.1509193/1‑150 (MQ=60)
                                                 AGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTT                                                                                                    <  SRR19973069.2783036/150‑1 (MQ=60)
                                                 AGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTT                                                                                                    <  SRR19973069.2782941/150‑1 (MQ=60)
                                                   CGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGC                                                                                                  <  SRR19973069.2060571/150‑1 (MQ=60)
                                                      TTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAAT                                                                                               <  SRR19973069.3784496/150‑1 (MQ=60)
                                                       TCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATT                                                                                              <  SRR19973069.2459451/150‑1 (MQ=60)
                                                       TCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATT                                                                                              <  SRR19973069.247581/150‑1 (MQ=60)
                                                       TCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATT                                                                                              >  SRR19973069.1022318/1‑150 (MQ=60)
                                                        CAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTG                                                                                             <  SRR19973069.1093581/150‑1 (MQ=60)
                                                        CAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTG                                                                                             >  SRR19973069.3282298/1‑150 (MQ=60)
                                                        CAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTG                                                                                             >  SRR19973069.3282337/1‑150 (MQ=60)
                                                           GCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTT                                                                                          <  SRR19973069.1813913/150‑1 (MQ=60)
                                                           GCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTT                                                                                          <  SRR19973069.1813908/150‑1 (MQ=60)
                                                           GCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTT                                                                                          <  SRR19973069.3514309/150‑1 (MQ=60)
                                                            CATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTA                                                                                         >  SRR19973069.510419/1‑150 (MQ=60)
                                                             ATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAA                                                                                        >  SRR19973069.1634288/1‑150 (MQ=60)
                                                               AACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATC                                                                                      <  SRR19973069.3626353/150‑1 (MQ=60)
                                                               AACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATC                                                                                      >  SRR19973069.3106856/1‑150 (MQ=60)
                                                               AACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATC                                                                                      >  SRR19973069.63778/1‑150 (MQ=60)
                                                                 CAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAA                                                                                    >  SRR19973069.2031810/1‑150 (MQ=60)
                                                                 CAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCCAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAA                                                                                    >  SRR19973069.2390273/1‑150 (MQ=60)
                                                                   ATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGT                                                                                  >  SRR19973069.1631486/1‑150 (MQ=60)
                                                                    TGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTT                                                                                 >  SRR19973069.962448/1‑150 (MQ=60)
                                                                     GGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTA                                                                                <  SRR19973069.234106/150‑1 (MQ=60)
                                                                      GAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTAC                                                                               >  SRR19973069.2845087/1‑150 (MQ=60)
                                                                       AACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACT                                                                              >  SRR19973069.3227181/1‑150 (MQ=60)
                                                                       AACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACT                                                                              <  SRR19973069.2070427/150‑1 (MQ=60)
                                                                       AACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACT                                                                              >  SRR19973069.3227214/1‑150 (MQ=60)
                                                                       AACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGNTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACT                                                                              >  SRR19973069.1947191/1‑150 (MQ=60)
                                                                       AACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGNTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACT                                                                              >  SRR19973069.1950094/1‑150 (MQ=60)
                                                                        ACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTG                                                                             <  SRR19973069.567881/150‑1 (MQ=60)
                                                                        ACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTG                                                                             <  SRR19973069.1738767/150‑1 (MQ=60)
                                                                          AAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCT                                                                           >  SRR19973069.2271114/1‑150 (MQ=60)
                                                                           AGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCTCTCTTGCAATTGTTTAATCAAGTTACTGCTT                                                                          <  SRR19973069.3665336/150‑1 (MQ=60)
                                                                           AGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTT                                                                          <  SRR19973069.3665441/150‑1 (MQ=60)
                                                                           AGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTT                                                                          <  SRR19973069.3665331/150‑1 (MQ=60)
                                                                               GCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCA                                                                      <  SRR19973069.1985587/150‑1 (MQ=60)
                                                                               GCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCA                                                                      <  SRR19973069.43066/150‑1 (MQ=60)
                                                                                  CCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCA                                                                   <  SRR19973069.873471/150‑1 (MQ=60)
                                                                                  CCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCA                                                                   >  SRR19973069.2656421/1‑150 (MQ=60)
                                                                                  CCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCA                                                                   >  SRR19973069.2656518/1‑150 (MQ=60)
                                                                                   CAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAA                                                                  >  SRR19973069.719848/1‑150 (MQ=60)
                                                                                       TGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATC                                                              <  SRR19973069.3383853/150‑1 (MQ=60)
                                                                                        GCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCT                                                             <  SRR19973069.375420/150‑1 (MQ=60)
                                                                                         CAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTC                                                            >  SRR19973069.165892/1‑150 (MQ=60)
                                                                                         CAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTC                                                            >  SRR19973069.3648038/1‑150 (MQ=60)
                                                                                         CAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTC                                                            >  SRR19973069.165836/1‑150 (MQ=60)
                                                                                          AAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCC                                                           >  SRR19973069.2884786/1‑150 (MQ=60)
                                                                                          AAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCC                                                           <  SRR19973069.3176106/150‑1 (MQ=60)
                                                                                          AAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCC                                                           >  SRR19973069.3782420/1‑150 (MQ=60)
                                                                                          AAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCC                                                           >  SRR19973069.960480/1‑150 (MQ=60)
                                                                                          AAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTAGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCC                                                           >  SRR19973069.3085411/1‑150 (MQ=60)
                                                                                            GCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCAT                                                         <  SRR19973069.3069796/150‑1 (MQ=60)
                                                                                            GCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCAT                                                         <  SRR19973069.3632167/150‑1 (MQ=60)
                                                                                             CTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATG                                                        <  SRR19973069.1115868/150‑1 (MQ=60)
                                                                                             CTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATG                                                        <  SRR19973069.1116022/150‑1 (MQ=60)
                                                                                               GTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTA                                                      >  SRR19973069.2260017/1‑150 (MQ=60)
                                                                                               GTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTA                                                      >  SRR19973069.2259362/1‑150 (MQ=60)
                                                                                               GTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTA                                                      >  SRR19973069.2259984/1‑150 (MQ=60)
                                                                                               GTAAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTA                                                      <  SRR19973069.2646756/150‑1 (MQ=60)
                                                                                               GTAAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTA                                                      <  SRR19973069.2643371/150‑1 (MQ=60)
                                                                                                  AGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATAT                                                   <  SRR19973069.1009999/150‑1 (MQ=60)
                                                                                                  AGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATAT                                                   <  SRR19973069.2954333/150‑1 (MQ=60)
                                                                                                  AGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATAT                                                   <  SRR19973069.2498103/150‑1 (MQ=60)
                                                                                                  AGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATAT                                                   <  SRR19973069.1008704/150‑1 (MQ=60)
                                                                                                   GCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATA                                                  <  SRR19973069.3786090/150‑1 (MQ=60)
                                                                                                     GGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAAC                                                >  SRR19973069.1720036/1‑150 (MQ=60)
                                                                                                     GGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAAC                                                >  SRR19973069.1565391/1‑150 (MQ=60)
                                                                                                     GGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAAC                                                >  SRR19973069.1565598/1‑150 (MQ=60)
                                                                                                     GGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAAC                                                >  SRR19973069.1999478/1‑150 (MQ=60)
                                                                                                     GGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAAC                                                <  SRR19973069.1556152/150‑1 (MQ=60)
                                                                                                     GGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAAC                                                >  SRR19973069.1299305/1‑150 (MQ=60)
                                                                                                     GGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAAC                                                >  SRR19973069.54829/1‑150 (MQ=60)
                                                                                                        AGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGAC                                             >  SRR19973069.4511/1‑150 (MQ=60)
                                                                                                        AGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGAC                                             >  SRR19973069.1187222/1‑150 (MQ=60)
                                                                                                        AGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGAC                                             >  SRR19973069.3105760/1‑150 (MQ=60)
                                                                                                            TCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCA                                         >  SRR19973069.2044065/1‑150 (MQ=60)
                                                                                                            TCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCA                                         >  SRR19973069.2043869/1‑150 (MQ=60)
                                                                                                            TCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGAATTTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCA                                         <  SRR19973069.2866408/150‑1 (MQ=60)
                                                                                                              GAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTAATGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACC                                       >  SRR19973069.3212905/1‑150 (MQ=60)
                                                                                                               AAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCT                                      >  SRR19973069.2417607/1‑150 (MQ=60)
                                                                                                               AAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCT                                      >  SRR19973069.28558/1‑150 (MQ=60)
                                                                                                                AGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTT                                     >  SRR19973069.2110950/1‑150 (MQ=60)
                                                                                                                      AATAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGG                               >  SRR19973069.1780772/1‑150 (MQ=60)
                                                                                                                        TAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTA                             >  SRR19973069.471995/1‑150 (MQ=60)
                                                                                                                        TAGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTA                             >  SRR19973069.472015/1‑150 (MQ=60)
                                                                                                                         AGAGCGCGATCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTAC                            >  SRR19973069.3310036/1‑150 (MQ=60)
                                                                                                                                  TCGGGCCTTGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCAATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTT                   <  SRR19973069.3092816/150‑1 (MQ=60)
                                                                                                                                  GCGGGCCTGGCGCCTGTTGCTCTGATACCGCTTCCGCGTCCGTCACCTGGGCTACCCGCCCCCGCTCTTGAATTTTTTTAATCATTTTACTGCTTGCCATAAAAATCTCCTTGTATATAACGACTGCACCTTTGCCGGTACACGACTTTT                   <  SRR19973069.2640572/150‑1 (MQ=60)
                                                                                                                                   CGGGCGTTGCGCCTGTTGCTCTGATAACGGTTCCTCGTCCGTCACGTGGGCTACCAGCCCCCGCTCTTGCATTTTTTTAATCAATTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTC                  <  SRR19973069.812025/150‑1 (MQ=60)
                                                                                                                                   CGGGCCTTGCGCCTGTTGCTGTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGAATTTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTC                  <  SRR19973069.812026/150‑1 (MQ=60)
                                                                                                                                    GGGCCTTGCGCCTGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGAAATTGTTTAATCAATTTACTGCTTGCCATAAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCG                 <  SRR19973069.835178/150‑1 (MQ=60)
                                                                                                                                          TGCGCCAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCATTTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGAT           <  SRR19973069.1166471/150‑1 (MQ=60)
                                                                                                                                               CAGTTGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAA      >  SRR19973069.648821/1‑150 (MQ=60)
                                                                                                                                                   TGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTCTTCGCCAGATGCGAAAGAG  >  SRR19973069.3005812/1‑150 (MQ=60)
                                                                                                                                                   TGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTATTGCAATTGTTTAATAATGTTACTGCTTGCCATCAAAATCTCCATTTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAG  <  SRR19973069.1713993/150‑1 (MQ=60)
                                                                                                                                                   GGCTCTGCTAACGCTCCCTCGGCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAAACAAGTAACTGCTTGCCAACAAAATCTCCAGGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAG  <  SRR19973069.448268/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
CCCGTCGCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAG  >  CP009273/1711250‑1711546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑