Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F78 I0 R1 626 241.3 10389380 77.7% 8072548 149.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,139,910 G→T 100% R1055L (CGA→CTA)  yegE → putative diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,139,9100GT96.2% 971.0 / 19.3 275R1055L (CGA→CTA) yegEputative diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base G (2/9);  new base T (143/121);  total (145/130)
Fisher's exact test for biased strand distribution p-value = 2.79e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 275 total aligned reads displayed.

GCCAGGTGGGCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCGACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTATTTCGCGA  >  CP009273/2139763‑2140056
                                                                                                                                                   |                                                                                                                                                  
gCCAGGTGGGCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTAc                                                                                                                                                  <  1:246699/150‑1 (MQ=255)
 ccAGGTGGGCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACt                                                                                                                                                 >  2:3592765/1‑150 (MQ=255)
  cAGGTGGGCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTc                                                                                                                                                <  2:5144080/150‑1 (MQ=255)
     gTGGGCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCggg                                                                                                                                             >  2:238594/1‑150 (MQ=255)
      tGGGCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGa                                                                                                                                            >  2:2505458/1‑150 (MQ=255)
      tGGGCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCGACTCGGGa                                                                                                                                            <  2:2917282/150‑1 (MQ=255)
       gggCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGAt                                                                                                                                           <  2:833077/150‑1 (MQ=255)
             cgATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAAc                                                                                                                                     <  1:3291955/150‑1 (MQ=255)
              gATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAAcc                                                                                                                                    >  2:2273825/1‑150 (MQ=255)
              gATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAAcc                                                                                                                                    <  2:3285148/150‑1 (MQ=255)
                tCTGCAAATCTTCCACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCAt                                                                                                                                  <  2:2657051/150‑1 (MQ=255)
                 cTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATc                                                                                                                                 <  1:5130142/150‑1 (MQ=255)
                   gCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGc                                                                                                                               >  2:475737/1‑150 (MQ=255)
                    cAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGcc                                                                                                                              <  2:270469/150‑1 (MQ=255)
                        tCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGc                                                                                                                          <  1:2862656/150‑1 (MQ=255)
                          ttCAACTAGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCa                                                                                                                        >  1:1260056/1‑150 (MQ=255)
                             aaCTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTc                                                                                                                     <  1:4899010/150‑1 (MQ=255)
                             aaCTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTc                                                                                                                     <  2:3631048/150‑1 (MQ=255)
                              aCTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCg                                                                                                                    >  2:2603333/1‑150 (MQ=255)
                              aCTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCg                                                                                                                    >  2:2603747/1‑150 (MQ=255)
                               cTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGt                                                                                                                   >  2:398753/1‑150 (MQ=255)
                                tCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGTATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGtt                                                                                                                  >  1:2302339/1‑150 (MQ=255)
                                 cGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTTATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGttt                                                                                                                 >  2:3764187/1‑150 (MQ=255)
                                    tGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTAc                                                                                                              >  1:2538561/1‑150 (MQ=255)
                                        aGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCtt                                                                                                          <  2:1667616/150‑1 (MQ=255)
                                           gAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGt                                                                                                       >  2:4861678/1‑150 (MQ=255)
                                            aaTATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTg                                                                                                      >  1:3374401/1‑150 (MQ=255)
                                            aaTATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTg                                                                                                      <  1:4616548/150‑1 (MQ=255)
                                              tatGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCGTAGTGAt                                                                                                    >  1:4563225/1‑150 (MQ=255)
                                                    aGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATAc                                                                                              <  2:3923544/150‑1 (MQ=255)
                                                    aGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATAc                                                                                              <  2:1812568/150‑1 (MQ=255)
                                                     gATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCGACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACg                                                                                             <  1:4738055/150‑1 (MQ=255)
                                                      aTTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGc                                                                                            <  1:4801439/150‑1 (MQ=255)
                                                      aTTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGc                                                                                            <  2:2184705/150‑1 (MQ=255)
                                                       ttACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCt                                                                                           <  2:1986132/150‑1 (MQ=255)
                                                       ttACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCt                                                                                           <  2:3967471/150‑1 (MQ=255)
                                                           cTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCt                                                                                       <  2:1518614/150‑1 (MQ=255)
                                                               tACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCa                                                                                   >  2:1032440/1‑150 (MQ=255)
                                                               tACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCa                                                                                   <  2:684698/150‑1 (MQ=255)
                                                               tACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCa                                                                                   >  1:3273869/1‑150 (MQ=255)
                                                                  ttGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCg                                                                                <  1:2906203/150‑1 (MQ=255)
                                                                     aTGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCg                                                                             <  1:115266/150‑1 (MQ=255)
                                                                      tGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGt                                                                            >  1:4319414/1‑150 (MQ=255)
                                                                       ggTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTc                                                                           <  2:309039/150‑1 (MQ=255)
                                                                       ggTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGAGGCTGATTACGATTAGTCAGGGGCACGCTCAGCGACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTc                                                                           <  2:3272693/150‑1 (MQ=255)
                                                                         tGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGa                                                                         >  2:2030817/1‑150 (MQ=255)
                                                                           aGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATc                                                                       >  2:3517295/1‑150 (MQ=255)
                                                                              tATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGa                                                                    >  2:3160664/1‑150 (MQ=255)
                                                                              tATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGa                                                                    <  1:3989689/150‑1 (MQ=255)
                                                                                   gcCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGTTGGATACGCTTTCTGGCATAGGCGTCGATCTGATTTAt                                                               >  1:4454220/1‑150 (MQ=255)
                                                                                   gcCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTAt                                                               >  1:5151609/1‑150 (MQ=255)
                                                                                    ccAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATg                                                              <  2:3685961/150‑1 (MQ=255)
                                                                                     cAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTAACCTTAGTGATGGATAAGCTTTCTGGCATCGGCGTCGATCTGATTTAttg                                                             >  1:4935495/1‑148 (MQ=255)
                                                                                      aaCGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATggt                                                            >  2:4479525/1‑150 (MQ=255)
                                                                                      aaCGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATggt                                                            >  2:4479295/1‑150 (MQ=255)
                                                                                        cGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATggtga                                                          >  2:4204488/1‑150 (MQ=255)
                                                                                          tGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATggtgagg                                                        <  1:2394609/150‑1 (MQ=255)
                                                                                           gCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATggtgaggt                                                       <  1:2025158/150‑1 (MQ=255)
                                                                                            cAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATggtgaggtg                                                      <  1:1975434/150‑1 (MQ=255)
                                                                                             aGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATggtgaggtga                                                     <  2:723441/150‑1 (MQ=255)
                                                                                              gggTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGAt                                                    <  2:654578/150‑1 (MQ=255)
                                                                                              gggTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGAt                                                    <  1:591988/150‑1 (MQ=255)
                                                                                               ggTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGAtt                                                   <  1:1365815/150‑1 (MQ=255)
                                                                                               ggTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCGACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGAtt                                                   <  2:2419905/150‑1 (MQ=255)
                                                                                                gTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCGTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTg                                                  >  2:27868/1‑150 (MQ=255)
                                                                                                     ttGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGAt                                             <  1:415654/150‑1 (MQ=255)
                                                                                                      tGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTTCCGATg                                            >  2:5059749/1‑150 (MQ=255)
                                                                                                        atggatgAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGcc                                          <  2:4613992/150‑1 (MQ=255)
                                                                                                         tggatgAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGccc                                         <  2:744427/150‑1 (MQ=255)
                                                                                                         tggatgAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATATGATTTATGGTGAGGTGATTGCCGATTccc                                         >  1:1718229/1‑150 (MQ=255)
                                                                                                          ggatgAGATGCTGATTACGATTATTCAGGGGAACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTAACCGTAGTGATGGATACGCTTTCTGGCAAAGGCGTCGGTGTGATTTATTGTGAGGGGGATGGCGATGCCCa                                        >  1:1771088/1‑150 (MQ=255)
                                                                                                            atgAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCGTAGTGATGGATACGCTTTCTTGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTTCCGATGCCCAAc                                      >  1:1103842/1‑150 (MQ=255)
                                                                                                              gagaTGCTGATTACGATTATTCAGGGGCACGCTCAGCGACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGTTTGACGATGCCCAAACg                                    >  2:433400/1‑150 (MQ=255)
                                                                                                               agaTGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGc                                   >  1:2922938/1‑150 (MQ=255)
                                                                                                                     tGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGAtt                             >  1:711977/1‑150 (MQ=255)
                                                                                                                     tGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGAtt                             >  2:856358/1‑150 (MQ=255)
                                                                                                                     tGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGGCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGAtt                             >  1:3854153/1‑150 (MQ=255)
                                                                                                                      gATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGAttt                            <  2:1378249/150‑1 (MQ=255)
                                                                                                                      gATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGAttt                            <  2:3549631/150‑1 (MQ=255)
                                                                                                                      gATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGAttt                            >  2:3171180/1‑150 (MQ=255)
                                                                                                                         tACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTATGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTTCCGATGCCCAACCGCTGGATTTGCt                         >  1:4378206/1‑150 (MQ=255)
                                                                                                                          aCGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTg                        >  1:3400859/1‑150 (MQ=255)
                                                                                                                              ttattCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGa                    <  2:421068/150‑1 (MQ=255)
                                                                                                                              ttattCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTGTTACCCTTAGTGATGGATACGCGTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGGGa                    >  1:307564/1‑150 (MQ=255)
                                                                                                                                  tCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAAtag                <  1:3654343/150‑1 (MQ=255)
                                                                                                                                   cAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAAtagt               >  2:4139943/1‑150 (MQ=255)
                                                                                                                                    aGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAAtagta              <  1:3567170/150‑1 (MQ=255)
                                                                                                                                    aGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAAtagta              >  2:283331/1‑150 (MQ=255)
                                                                                                                                    aGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAAtagta              <  1:1996497/150‑1 (MQ=255)
                                                                                                                                         cACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTAt         <  1:2705834/150‑1 (MQ=255)
                                                                                                                                          tcGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTAtt        <  1:4302045/149‑1 (MQ=255)
                                                                                                                                          aCGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTAtt        <  1:2530463/150‑1 (MQ=255)
                                                                                                                                          aCGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCGTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTAtt        >  1:5103137/1‑150 (MQ=255)
                                                                                                                                          aCGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCGTAGTGATGGGTACGCTTTCTGGCAGCGGCGGCGATGTGATTTATTGTGTGGTGATTGCCGATTGCCAACCGCGGGCTTTGCTGGGGTATAGTAGTTAtt        >  1:2065963/1‑150 (MQ=255)
                                                                                                                                           cGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTAttt       <  1:1595202/150‑1 (MQ=255)
                                                                                                                                           cGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTAACCATAGTGTTGGATACGCTTTAAGGCAACGGCGGCGATGTGGTTTATTGTTGGGTGATTTACGATGCCCAACCCCTGGAATTGATGGTGTATAGTatttattt       >  1:2354985/1‑150 (MQ=255)
                                                                                                                                               cAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTATTTcgcg   >  2:368310/1‑150 (MQ=255)
                                                                                                                                                aGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTATTTCGCGa  <  2:1312165/150‑1 (MQ=255)
                                                                                                                                                aGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTATTTCGCGa  <  2:3867609/150‑1 (MQ=255)
                                                                                                                                                aGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTGTTAACCGTAGGGGTGGTTACGCTTTCAGGCCACGGCGTCGATGTGAGGTATGGGGTGGTGTTTGGCGATGCCCCACCCCGGGATTTGCTGGGGTATAGTGGTTATTTCGCGa  >  1:1900887/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCCAGGTGGGCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCGACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTATTTCGCGA  >  CP009273/2139763‑2140056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCCAGGTGGGCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCGACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTATTTCGCGATTA  >  CP009273/2139763‑2140059
                                                                                                                                                   |                                                                                                                                                     
GCCAGGTGGGCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTAC                                                                                                                                                     <  SRR19973083.248030/150‑1 (MQ=60)
GCCAGGTGGGCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTAC                                                                                                                                                     <  SRR19973083.247810/150‑1 (MQ=60)
 CCAGGTGGGCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACT                                                                                                                                                    <  SRR19973083.873459/150‑1 (MQ=60)
    GGTGGGCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGG                                                                                                                                                 >  SRR19973083.591924/1‑150 (MQ=60)
       GGGCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGAT                                                                                                                                              >  SRR19973083.4367063/1‑150 (MQ=60)
        GGCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATG                                                                                                                                             >  SRR19973083.3033478/1‑150 (MQ=60)
             CGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAAC                                                                                                                                        <  SRR19973083.3307443/150‑1 (MQ=60)
             CGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAAC                                                                                                                                        >  SRR19973083.2522112/1‑150 (MQ=60)
               ATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCA                                                                                                                                      >  SRR19973083.4701919/1‑150 (MQ=60)
               ATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCA                                                                                                                                      >  SRR19973083.4701939/1‑150 (MQ=60)
                 CTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATC                                                                                                                                    <  SRR19973083.5151977/150‑1 (MQ=60)
                   GCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGC                                                                                                                                  <  SRR19973083.1796480/150‑1 (MQ=60)
                       ATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGG                                                                                                                              >  SRR19973083.2823251/1‑150 (MQ=60)
                       ATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGG                                                                                                                              <  SRR19973083.5165197/150‑1 (MQ=60)
                        TCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGC                                                                                                                             <  SRR19973083.2876758/150‑1 (MQ=60)
                          TTCAACTAGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCA                                                                                                                           >  SRR19973083.1266192/1‑150 (MQ=60)
                             AACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTC                                                                                                                        <  SRR19973083.4920072/150‑1 (MQ=60)
                             AACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTC                                                                                                                        <  SRR19973083.3442995/150‑1 (MQ=60)
                             AACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTC                                                                                                                        >  SRR19973083.4632981/1‑150 (MQ=60)
                              ACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCG                                                                                                                       >  SRR19973083.2045432/1‑150 (MQ=60)
                                TCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGTATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTT                                                                                                                     >  SRR19973083.2314134/1‑150 (MQ=60)
                                    TGAAAGCTAATTTGGCAGTTTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCTGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCTGCTACTCGGGATGTAAACCATCGCCGGGCCTGTCGTTTTAC                                                                                                                 <  SRR19973083.5151808/150‑1 (MQ=60)
                                    TGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTAC                                                                                                                 >  SRR19973083.2551274/1‑150 (MQ=60)
                                    TGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTAC                                                                                                                 >  SRR19973083.2551437/1‑150 (MQ=60)
                                        AGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTT                                                                                                             <  SRR19973083.1178306/150‑1 (MQ=60)
                                         GCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTA                                                                                                            <  SRR19973083.4108407/150‑1 (MQ=60)
                                          CGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAG                                                                                                           <  SRR19973083.4172354/150‑1 (MQ=60)
                                            AATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTG                                                                                                         >  SRR19973083.3390870/1‑150 (MQ=60)
                                            AATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTG                                                                                                         >  SRR19973083.3390158/1‑150 (MQ=60)
                                            AATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTG                                                                                                         >  SRR19973083.3390069/1‑150 (MQ=60)
                                            AATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTG                                                                                                         <  SRR19973083.4636477/150‑1 (MQ=60)
                                            AATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTG                                                                                                         >  SRR19973083.3775769/1‑150 (MQ=60)
                                            AATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTG                                                                                                         >  SRR19973083.774956/1‑150 (MQ=60)
                                             ATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGA                                                                                                        <  SRR19973083.2018125/150‑1 (MQ=60)
                                              TATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGAT                                                                                                       >  SRR19973083.4582934/1‑150 (MQ=60)
                                              TATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGAT                                                                                                       >  SRR19973083.4212797/1‑150 (MQ=60)
                                              TATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCGTAGTGAT                                                                                                       >  SRR19973083.4582974/1‑150 (MQ=60)
                                              TATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTGTTACCCTTAGTGAT                                                                                                       >  SRR19973083.3657616/1‑150 (MQ=60)
                                                   CAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATA                                                                                                  <  SRR19973083.4437633/150‑1 (MQ=60)
                                                   CAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCGTAGTGATGGATA                                                                                                  >  SRR19973083.2040861/1‑150 (MQ=60)
                                                     GATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCATAGTGATGGATACG                                                                                                >  SRR19973083.2588288/1‑150 (MQ=60)
                                                     GATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCGACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACG                                                                                                <  SRR19973083.239555/150‑1 (MQ=60)
                                                     GATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCGACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACG                                                                                                <  SRR19973083.4758289/150‑1 (MQ=60)
                                                      ATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGC                                                                                               <  SRR19973083.2227990/150‑1 (MQ=60)
                                                      ATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGC                                                                                               >  SRR19973083.3369115/1‑150 (MQ=60)
                                                      ATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGC                                                                                               <  SRR19973083.4821977/150‑1 (MQ=60)
                                                               TACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCA                                                                                      >  SRR19973083.3289300/1‑150 (MQ=60)
                                                               TACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCA                                                                                      >  SRR19973083.3289301/1‑150 (MQ=60)
                                                                ACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCAT                                                                                     >  SRR19973083.1947669/1‑150 (MQ=60)
                                                                  TTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCG                                                                                   <  SRR19973083.2920388/150‑1 (MQ=60)
                                                                  TTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCG                                                                                   <  SRR19973083.2920434/150‑1 (MQ=60)
                                                                  TTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCG                                                                                   <  SRR19973083.2920380/150‑1 (MQ=60)
                                                                   TGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGG                                                                                  >  SRR19973083.4931784/1‑150 (MQ=60)
                                                                     ATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCG                                                                                <  SRR19973083.116009/150‑1 (MQ=60)
                                                                      TGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGT                                                                               >  SRR19973083.1734131/1‑150 (MQ=60)
                                                                      TGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGT                                                                               >  SRR19973083.4338170/1‑150 (MQ=60)
                                                                      TGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTGTTACCCGTAGTGAATGATACGCTTTCTGGCATCGGCGT                                                                               >  SRR19973083.160090/1‑150 (MQ=60)
                                                                       GGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATTGATAAGCTTTCTGGCAAAGGCGTC                                                                              >  SRR19973083.2490988/1‑150 (MQ=60)
                                                                       GGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTC                                                                              <  SRR19973083.70335/150‑1 (MQ=60)
                                                                           AGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATC                                                                          <  SRR19973083.2921869/150‑1 (MQ=60)
                                                                           AGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATC                                                                          >  SRR19973083.5161337/1‑150 (MQ=60)
                                                                           AGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCGTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATC                                                                          >  SRR19973083.1505538/1‑150 (MQ=60)
                                                                              TATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGA                                                                       <  SRR19973083.4007440/150‑1 (MQ=60)
                                                                              TATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGA                                                                       <  SRR19973083.4007436/150‑1 (MQ=60)
                                                                              TATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCGTAGTGATGGATACGCTTTCTGGCATCGGCGGCGATCTGA                                                                       >  SRR19973083.4408000/1‑150 (MQ=60)
                                                                               ATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATAAGCTTTCTGGCATCGGCGTCGATCTGAT                                                                      >  SRR19973083.4369951/1‑150 (MQ=60)
                                                                               ATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCGTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGAT                                                                      >  SRR19973083.2330916/1‑150 (MQ=60)
                                                                                   GCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGTTGGATACGCTTTCTGGCATAGGCGTCGATCTGATTTAT                                                                  >  SRR19973083.4473579/1‑150 (MQ=60)
                                                                                   GCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTAT                                                                  >  SRR19973083.5173486/1‑150 (MQ=60)
                                                                                     CAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTAACCTTAGTGATGGATAAGCTTTCTGGCATCGGCGTCGATCTGATTTATTG                                                                >  SRR19973083.4956719/1‑150 (MQ=60)
                                                                                      AACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGAAGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGT                                                               >  SRR19973083.1726741/1‑150 (MQ=60)
                                                                                          TGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGG                                                           <  SRR19973083.2406918/150‑1 (MQ=60)
                                                                                           GCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGT                                                          <  SRR19973083.2035505/150‑1 (MQ=60)
                                                                                            CAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTG                                                         <  SRR19973083.1985527/150‑1 (MQ=60)
                                                                                              GGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGAT                                                       <  SRR19973083.594963/150‑1 (MQ=60)
                                                                                               GGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATT                                                      <  SRR19973083.1372565/150‑1 (MQ=60)
                                                                                               GGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATT                                                      <  SRR19973083.4957358/150‑1 (MQ=60)
                                                                                               GGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATT                                                      <  SRR19973083.1953718/150‑1 (MQ=60)
                                                                                                     TTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGAT                                                <  SRR19973083.417520/150‑1 (MQ=60)
                                                                                                         TGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCC                                            >  SRR19973083.1726637/1‑150 (MQ=60)
                                                                                                         TGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCC                                            >  SRR19973083.4331171/1‑150 (MQ=60)
                                                                                                         TGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATATGATTTATGGTGAGGTGATTGCCGATTCCC                                            >  SRR19973083.1726662/1‑150 (MQ=60)
                                                                                                          GGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTAACCGTAGTGATGGCTACGCGTTCTGGCCTCGGCGTCGATCgggtgtattgtggggtggtttgcgaagcccc                                           >  SRR19973083.1779920/1‑119 (MQ=60)
                                                                                                          GGATGAGATGCTGATTACGATTATTCAGGGGAACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTAACCGTAGTGATGGATACGCTTTCTGGCAAAGGCGTCGGTGTGATTTATTGTGAGGGGGATGGCGATGCCCA                                           >  SRR19973083.1779870/1‑150 (MQ=60)
                                                                                                            ATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCGTAGTGATGGATACGCTTTCTTGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTTCCGATGCCCAAC                                         >  SRR19973083.1109392/1‑150 (MQ=60)
                                                                                                            ATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTAACCGTAGTGATTGATAAGCTTTCTGGCAACGGCGTCGATCTGATTTATGGTGTGGTGATTGACGATTCCCAAC                                         >  SRR19973083.2242059/1‑150 (MQ=60)
                                                                                                               AGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGC                                      >  SRR19973083.2937238/1‑150 (MQ=60)
                                                                                                                GATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCT                                     <  SRR19973083.2422842/150‑1 (MQ=60)
                                                                                                                GATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCT                                     <  SRR19973083.477959/150‑1 (MQ=60)
                                                                                                                GATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCT                                     <  SRR19973083.477991/150‑1 (MQ=60)
                                                                                                                GATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCA                                     >  SRR19973083.304622/1‑150 (MQ=60)
                                                                                                                     TGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATT                                <  SRR19973083.1342643/150‑1 (MQ=60)
                                                                                                                     TGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATT                                <  SRR19973083.1342671/150‑1 (MQ=60)
                                                                                                                     TGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATT                                >  SRR19973083.4873914/1‑150 (MQ=60)
                                                                                                                     TGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATT                                >  SRR19973083.715515/1‑150 (MQ=60)
                                                                                                                     TGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGGCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATT                                >  SRR19973083.3871422/1‑150 (MQ=60)
                                                                                                                     TGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCGTAGTGATGGATACGCTTTATTGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCCAACCCTGGATT                                >  SRR19973083.4873868/1‑150 (MQ=60)
                                                                                                                     TGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTAACCATAGTGATGGATACGCTTTAAGGCAACGGCGTCGATCAGAATTATGGGGAGGGGATTGACCATTCCCAACCCCTGGAAT                                >  SRR19973083.715285/1‑150 (MQ=60)
                                                                                                                         TACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCT                            <  SRR19973083.3299606/150‑1 (MQ=60)
                                                                                                                         TACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTATGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTTCCGATGCCCAACCGCTGGATTTGCT                            >  SRR19973083.4397232/1‑150 (MQ=60)
                                                                                                                          ACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTG                           >  SRR19973083.3416581/1‑150 (MQ=60)
                                                                                                                            GATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGT                         <  SRR19973083.4291590/150‑1 (MQ=60)
                                                                                                                            GATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCGTAGTGATGGATACGCTTTCTGGCATCGGCGGCGATCGGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGT                         >  SRR19973083.1140294/1‑150 (MQ=60)
                                                                                                                              TTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTGTTACCCTTAGTGATGGATACGCGTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGGGA                       >  SRR19973083.309136/1‑150 (MQ=60)
                                                                                                                               TATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAA                      <  SRR19973083.3132678/150‑1 (MQ=60)
                                                                                                                                ATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATAAGCTTTATGGCATCGGCGTCGATCGGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAAT                     >  SRR19973083.342133/1‑150 (MQ=60)
                                                                                                                                 TTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATA                    <  SRR19973083.1208004/150‑1 (MQ=60)
                                                                                                                                  TCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAG                   <  SRR19973083.3671049/150‑1 (MQ=60)
                                                                                                                                    AGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTA                 <  SRR19973083.2006699/150‑1 (MQ=60)
                                                                                                                                    AGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTA                 <  SRR19973083.3128032/150‑1 (MQ=60)
                                                                                                                                    AGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTA                 <  SRR19973083.3583533/150‑1 (MQ=60)
                                                                                                                                    AGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTA                 <  SRR19973083.3583997/150‑1 (MQ=60)
                                                                                                                                    AGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTA                 <  SRR19973083.2006724/150‑1 (MQ=60)
                                                                                                                                         CACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTAT            <  SRR19973083.2719398/150‑1 (MQ=60)
                                                                                                                                         CACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTAT            <  SRR19973083.2719374/150‑1 (MQ=60)
                                                                                                                                          TCGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTATT           <  SRR19973083.4320764/150‑1 (MQ=60)
                                                                                                                                          ACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTATT           <  SRR19973083.2543306/150‑1 (MQ=60)
                                                                                                                                          ACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTATT           >  SRR19973083.5125116/1‑150 (MQ=60)
                                                                                                                                          ACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTATT           >  SRR19973083.5124868/1‑150 (MQ=60)
                                                                                                                                          ACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCGTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTATT           >  SRR19973083.5124902/1‑150 (MQ=60)
                                                                                                                                          ACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCGTAGTGATGGGTACGCTTTCTGGCAGCGGCGGCGATGTGATTTATTGTGTGGTGATTGCCGATTGCCAACCGCGGGCTTTGCTGGGGTATAGTAGTTATT           >  SRR19973083.2076478/1‑150 (MQ=60)
                                                                                                                                           CGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTATTT          <  SRR19973083.1603191/150‑1 (MQ=60)
                                                                                                                                           CGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCGTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGTGGTGTTTGCCGATGCCCCACCCCGGGCTTTTCTGGTGAATAGTAGTTATTT          >  SRR19973083.2367269/1‑150 (MQ=60)
                                                                                                                                           CGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTAACCATAGTGTTGGATACGCTTTAAGGCAACGGCGGCGATGTGGTTTATTGTTGGGTGATTTACGATGCCCAACCCCTGGAATTGATGGTGTATAGTATTTATTT          >  SRR19973083.2367118/1‑150 (MQ=38)
                                                                                                                                              TCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTATTTCGC       <  SRR19973083.3909963/150‑1 (MQ=60)
                                                                                                                                               CAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTTCTGGTGAATAGTAGTTTTTTAGCG      >  SRR19973083.1996209/1‑150 (MQ=60)
                                                                                                                                               CAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGAACTGATTTATGGTGAGGTGATTTCCGATGCCCAACCCCTGGATTTGCTGGGGAATAGTAGTTATTTCGCG      >  SRR19973083.2643561/1‑150 (MQ=60)
                                                                                                                                                AGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCGTAGTGATGGATACGCTTTATGGCATCGGCGGCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTATTTCGCGA     >  SRR19973083.4509015/1‑150 (MQ=60)
                                                                                                                                                AGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTGTTAACCGTAGGGGTGGTTACGCTTTCAGGCCACGGCGTCGATGTGAGGTATGGGGTGGTGTTTGGCGATGCCCCACCCCGGGATTTGCTGGGGTATAGTGGTTATTTCGCGA     >  SRR19973083.1910537/1‑150 (MQ=19)
                                                                                                                                                 GCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTATTTCGCGAT    <  SRR19973083.3707182/150‑1 (MQ=60)
                                                                                                                                                 GCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTATTTCGCGAT    <  SRR19973083.3707466/150‑1 (MQ=60)
                                                                                                                                                  CTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTGTTAACCGTAGTGATGGATACGCTTTCTGGCAACGGCGGCGATCTGATTTATTGTGAGGGGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGGATAGTAGTTATTTAGCGATT   >  SRR19973083.1163372/1‑150 (MQ=60)
                                                                                                                                                   TACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTATTTCGCGATTA  <  SRR19973083.875284/150‑1 (MQ=60)
                                                                                                                                                   TACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTATTTCGCGATTA  <  SRR19973083.286094/150‑1 (MQ=60)
                                                                                                                                                   TACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTATTTCGCGATTA  <  SRR19973083.286120/150‑1 (MQ=60)
                                                                                                                                                   TACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTATTTCGCGATTA  <  SRR19973083.4418100/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
GCCAGGTGGGCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCGACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTATTTCGCGATTA  >  CP009273/2139763‑2140059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑