Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,139,910 G→T 100% R1055L (CGA→CTA)  yegE → putative diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,139,9100GT98.6% 707.3 / 0.4 219R1055L (CGA→CTA) yegEputative diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base T (85/131);  total (86/133)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 220 total aligned reads displayed.

CCAGGTGGGCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCGACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTATTTCGCGAT  >  CP009273/2139764‑2140057
                                                                                                                                                  |                                                                                                                                                   
  aGGTGGGCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACt                                                                                                                                                  <  2:947329/148‑1 (MQ=255)
  aGGTGGGCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACt                                                                                                                                                  <  2:2233420/148‑1 (MQ=255)
   ggTGGGCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTc                                                                                                                                                 >  1:3310920/1‑148 (MQ=255)
   ggTGGGCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTc                                                                                                                                                 <  1:1544348/148‑1 (MQ=255)
          cgcgATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGa                                                                                                                                          <  1:2527829/148‑1 (MQ=255)
          cgcgATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGa                                                                                                                                          <  1:765744/148‑1 (MQ=255)
          cgcgATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGa                                                                                                                                          <  2:1335864/148‑1 (MQ=255)
              aTCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAAc                                                                                                                                      <  2:1034995/148‑1 (MQ=255)
                cTGCAAATCTTCAACTCGCTGAAAGCGAATAGGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCa                                                                                                                                    <  2:108288/148‑1 (MQ=255)
                 tGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCAt                                                                                                                                   >  1:2239522/1‑148 (MQ=255)
                    aaaTCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGc                                                                                                                                >  1:2679905/1‑148 (MQ=255)
                          tCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGcc                                                                                                                          >  1:1581390/1‑148 (MQ=255)
                            aaCTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAg                                                                                                                        <  2:565152/148‑1 (MQ=255)
                             aCTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGt                                                                                                                       >  2:1849477/1‑148 (MQ=255)
                             aCTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGt                                                                                                                       <  2:2285102/148‑1 (MQ=255)
                                cGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATTGTGATGTTTGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTTCTCGGGATGAAAACCATCGCCGGGCCAGTCGt                                                                                                                    <  2:80317/148‑1 (MQ=255)
                                cGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGt                                                                                                                    <  2:1356895/148‑1 (MQ=255)
                                cGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGt                                                                                                                    <  2:80402/148‑1 (MQ=255)
                                  cTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGttt                                                                                                                  >  1:942659/1‑148 (MQ=255)
                                  cTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGttt                                                                                                                  >  1:491469/1‑148 (MQ=255)
                                    gAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTa                                                                                                                <  2:2756950/148‑1 (MQ=255)
                                    gAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTa                                                                                                                >  1:2789130/1‑148 (MQ=255)
                                           aaTATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAg                                                                                                         <  2:2232853/148‑1 (MQ=255)
                                            atatGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGt                                                                                                        >  1:1095540/1‑148 (MQ=255)
                                             tatGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTg                                                                                                       >  2:117185/1‑148 (MQ=255)
                                             tatGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTg                                                                                                       <  1:1958801/148‑1 (MQ=255)
                                             tatGGCAGATTACCTGCTACTTGATGGTGAGTTATGCACCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTg                                                                                                       <  2:2409093/148‑1 (MQ=255)
                                               tGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGAt                                                                                                     <  2:2047268/148‑1 (MQ=255)
                                                ggCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATg                                                                                                    >  2:707260/1‑148 (MQ=255)
                                                ggCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATg                                                                                                    <  2:775946/148‑1 (MQ=255)
                                                  cAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGa                                                                                                  <  1:542169/148‑1 (MQ=255)
                                                    gATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATa                                                                                                <  1:1308803/148‑1 (MQ=255)
                                                    gATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATa                                                                                                <  1:1413470/148‑1 (MQ=255)
                                                    gATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATa                                                                                                <  1:3122014/148‑1 (MQ=255)
                                                    gATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATa                                                                                                >  2:1744670/1‑148 (MQ=255)
                                                     aTTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATAc                                                                                               >  2:1632921/1‑148 (MQ=255)
                                                       tACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGTTGGATACGc                                                                                             >  1:3027527/1‑148 (MQ=255)
                                                       tACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGc                                                                                             <  2:2395181/148‑1 (MQ=255)
                                                        aCCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCt                                                                                            <  1:1155057/148‑1 (MQ=255)
                                                         ccTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCtt                                                                                           >  2:1928988/1‑148 (MQ=255)
                                                         ccTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCtt                                                                                           >  2:504041/1‑148 (MQ=255)
                                                           tGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTc                                                                                         <  2:2790193/148‑1 (MQ=255)
                                                            gCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCt                                                                                        >  2:1915349/1‑148 (MQ=255)
                                                              tACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTgg                                                                                      >  1:3469430/1‑148 (MQ=255)
                                                              tACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTAACCGTAGTGATGGATACGCTTTCTgg                                                                                      >  1:2751820/1‑148 (MQ=255)
                                                               aCTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGc                                                                                     <  1:2567759/148‑1 (MQ=255)
                                                                  tGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATc                                                                                  <  2:3215087/148‑1 (MQ=255)
                                                                     tGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGc                                                                               <  1:1671493/148‑1 (MQ=255)
                                                                      ggTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCg                                                                              <  2:2918890/148‑1 (MQ=255)
                                                                      ggTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCg                                                                              <  1:2426451/148‑1 (MQ=255)
                                                                       gTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGt                                                                             <  1:2284488/148‑1 (MQ=255)
                                                                          aGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGa                                                                          <  2:1636469/148‑1 (MQ=255)
                                                                           gTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGAt                                                                         <  2:1392424/148‑1 (MQ=255)
                                                                             tATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCt                                                                       <  2:1097665/148‑1 (MQ=255)
                                                                             tATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCt                                                                       <  2:1096545/148‑1 (MQ=255)
                                                                                gcgcCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGAt                                                                    <  1:3408533/148‑1 (MQ=255)
                                                                                   ccAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTa                                                                 <  1:1967450/148‑1 (MQ=255)
                                                                                      aCGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATgg                                                              <  1:3252973/148‑1 (MQ=255)
                                                                                        gTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATggtg                                                            <  2:2359779/148‑1 (MQ=255)
                                                                                            aGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATggtgaggt                                                        <  1:854796/148‑1 (MQ=255)
                                                                                             gggTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATggtgaggtg                                                       >  2:3479581/1‑148 (MQ=255)
                                                                                              ggTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATAAGCTTTCTGGCATCGGCGTCGATGTGATTTATggtgaggtga                                                      >  1:2466612/1‑148 (MQ=255)
                                                                                              ggTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTAACCATAGTGATGGATAAGCTTTCTGGCCTCGGCGTCGAAGTGGTTTATggttttgtgt                                                      >  1:1607579/1‑141 (MQ=255)
                                                                                               gTAATTTGATGGTTGAGATGCTGATTAGGATTATTCTGGGGCACGCTCAGCTACTCGGGATGAAATCCATCGCCGGGCCAGTCGTTTTTCCCTTTTTGATTTATACGCTTTCTGGCATCGGAGTCGATCTGATTTATGGTGAGGTGAt                                                     <  1:1425391/148‑1 (MQ=255)
                                                                                               gTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGAt                                                     >  2:3281860/1‑148 (MQ=255)
                                                                                               gTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGAt                                                     >  2:1606367/1‑148 (MQ=255)
                                                                                               gTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGAt                                                     >  2:1536562/1‑148 (MQ=255)
                                                                                                tAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGAtt                                                    >  2:478141/1‑148 (MQ=255)
                                                                                                   tttGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTAACCGTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCGGATTTATGGTGAGGTGATTGcc                                                 >  2:2405036/1‑148 (MQ=255)
                                                                                                       atggatgAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATg                                             <  2:2189075/148‑1 (MQ=255)
                                                                                                       atggatgAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATg                                             <  2:2186534/148‑1 (MQ=255)
                                                                                                          gatgAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGccc                                          >  2:635735/1‑148 (MQ=255)
                                                                                                           atgAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCa                                         <  2:782194/148‑1 (MQ=255)
                                                                                                            tgAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCaa                                        <  2:168896/148‑1 (MQ=255)
                                                                                                             gagaTGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAAc                                       <  2:1984397/148‑1 (MQ=255)
                                                                                                               gaTGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCg                                     <  1:2986938/148‑1 (MQ=255)
                                                                                                                aTGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGc                                    <  2:3317110/148‑1 (MQ=255)
                                                                                                                 tGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCt                                   <  1:706901/148‑1 (MQ=255)
                                                                                                                  gCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTGTTACCCATAGTGATGGATACGCTTTCTTGCATCGGCGTCGCTCTGCTTTATTGTGGTGTGATTTCCGAATCCCAACCGCTg                                  >  1:2714897/1‑148 (MQ=255)
                                                                                                                     gATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGAt                               <  2:838625/148‑1 (MQ=255)
                                                                                                                     gATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGAt                               >  1:1767126/1‑148 (MQ=255)
                                                                                                                     gATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTAACCGTAGTGTTGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTTCCGAATCCCAACCGCTGGAt                               >  1:1066231/1‑148 (MQ=255)
                                                                                                                        tACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTg                            >  2:266276/1‑148 (MQ=255)
                                                                                                                          cGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCt                          <  2:3125214/148‑1 (MQ=255)
                                                                                                                          cGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCt                          <  1:291840/148‑1 (MQ=255)
                                                                                                                            attattCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCGTAGTGATTGATAAGCTTTCTGGCATCGGCGGCCATCTGAGTTTTGGTGAGGTGATTGCCGAATCCCAACCCCTGGAGTTGCTgg                        >  1:2844689/1‑148 (MQ=255)
                                                                                                                            attattCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCGTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTgg                        >  2:447107/1‑148 (MQ=255)
                                                                                                                             ttattCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGt                       >  1:2647593/1‑148 (MQ=255)
                                                                                                                             ttattCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGt                       <  2:1310307/148‑1 (MQ=255)
                                                                                                                             ttattCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCGTAGTGATGGATACGCTTTATGGCATCGGCGTCGATGGGATTTATGGTGAGGTGATTGGCGAATCCCAACCGCTGGATTTGCTGGt                       >  1:2172753/1‑148 (MQ=255)
                                                                                                                             ttattCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTGTTACCCGTGGTGTTGGATTCGCTGTATGGGATCGGCGTCGTGCGGCTGTATTGGGTGGTGTTTGGCGATGCCCAACCGCTGGGGTTGCTGGt                       >  1:2947218/1‑148 (MQ=255)
                                                                                                                               attCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGa                     <  1:117185/148‑1 (MQ=255)
                                                                                                                                 tCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAAt                   <  1:2183209/148‑1 (MQ=255)
                                                                                                                                  cAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAAta                  <  1:409033/148‑1 (MQ=255)
                                                                                                                                  cAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAAta                  <  1:1634256/148‑1 (MQ=255)
                                                                                                                                      ggCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAAtagtag              <  2:470037/148‑1 (MQ=255)
                                                                                                                                       gCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATagtagt             <  2:3133372/148‑1 (MQ=255)
                                                                                                                                          cGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTAt          <  2:2679905/148‑1 (MQ=255)
                                                                                                                                           gCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTAtt         <  1:2139020/148‑1 (MQ=255)
                                                                                                                                                  gACTCGGGATGACAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGAGTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTATTTCGCGAt  <  2:385255/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CCAGGTGGGCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCGACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTATTTCGCGAT  >  CP009273/2139764‑2140057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCAGGTGGGCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCGACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTATTTCGCGATTA  >  CP009273/2139764‑2140059
                                                                                                                                                  |                                                                                                                                                     
CCAGGTGGGCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACT                                                                                                                                                    <  SRR19973065.2068332/150‑1 (MQ=60)
CCAGGTGGGCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACT                                                                                                                                                    <  SRR19973065.2069482/150‑1 (MQ=60)
 CAGGTGGGCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTC                                                                                                                                                   >  SRR19973065.3331095/1‑150 (MQ=60)
   GGTGGGCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGG                                                                                                                                                 <  SRR19973065.1554560/150‑1 (MQ=60)
     TGGGCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCGACTCGGGA                                                                                                                                               >  SRR19973065.2575722/1‑150 (MQ=60)
          CGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAA                                                                                                                                          <  SRR19973065.770834/150‑1 (MQ=60)
          CGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAA                                                                                                                                          <  SRR19973065.2543696/150‑1 (MQ=60)
             GATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACC                                                                                                                                       >  SRR19973065.1610984/1‑150 (MQ=60)
               TCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCAT                                                                                                                                     >  SRR19973065.797623/1‑150 (MQ=60)
               TCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCAT                                                                                                                                     >  SRR19973065.2253760/1‑150 (MQ=60)
                  GCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGC                                                                                                                                  >  SRR19973065.2696522/1‑150 (MQ=60)
                        CTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCC                                                                                                                            >  SRR19973065.1591937/1‑150 (MQ=60)
                            AACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTC                                                                                                                        >  SRR19973065.30733/1‑150 (MQ=60)
                            AACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTC                                                                                                                        >  SRR19973065.1564925/1‑150 (MQ=60)
                                CGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTT                                                                                                                    >  SRR19973065.494781/1‑150 (MQ=60)
                                CGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTT                                                                                                                    >  SRR19973065.948886/1‑150 (MQ=60)
                                CGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTGT                                                                                                                    >  SRR19973065.949477/1‑150 (MQ=60)
                                  CTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTA                                                                                                                  >  SRR19973065.2806418/1‑150 (MQ=60)
                                  CTGAAAGCGAATATAGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTA                                                                                                                  >  SRR19973065.2807487/1‑150 (MQ=60)
                                    GAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACC                                                                                                                <  SRR19973065.3491787/150‑1 (MQ=60)
                                      AAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCT                                                                                                              >  SRR19973065.1123420/1‑150 (MQ=60)
                                      AAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTGTTACCCT                                                                                                              >  SRR19973065.2885059/1‑150 (MQ=60)
                                          GAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGT                                                                                                          <  SRR19973065.3477834/150‑1 (MQ=60)
                                          GAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGT                                                                                                          >  SRR19973065.1102749/1‑150 (MQ=60)
                                           AATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTG                                                                                                         <  SRR19973065.597419/150‑1 (MQ=60)
                                             TATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGAT                                                                                                       <  SRR19973065.1971943/150‑1 (MQ=60)
                                             TATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGAT                                                                                                       <  SRR19973065.1971597/150‑1 (MQ=60)
                                               TGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGG                                                                                                     <  SRR19973065.2613582/150‑1 (MQ=60)
                                                GGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGA                                                                                                    >  SRR19973065.2888913/1‑150 (MQ=60)
                                                GGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTAACCGTAGTGATGGA                                                                                                    >  SRR19973065.155503/1‑150 (MQ=60)
                                                 GCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGAT                                                                                                   <  SRR19973065.861251/150‑1 (MQ=60)
                                                  CAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATA                                                                                                  <  SRR19973065.545685/150‑1 (MQ=60)
                                                    GATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACG                                                                                                <  SRR19973065.1082787/150‑1 (MQ=60)
                                                    GATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACG                                                                                                <  SRR19973065.1422706/150‑1 (MQ=60)
                                                    GATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACG                                                                                                <  SRR19973065.3141025/150‑1 (MQ=60)
                                                    GATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACG                                                                                                <  SRR19973065.1317441/150‑1 (MQ=60)
                                                    GATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTGTTACCCTTAGTGATGGATACG                                                                                                >  SRR19973065.2369844/1‑150 (MQ=60)
                                                     ATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGTTGGATACGC                                                                                               >  SRR19973065.3046078/1‑150 (MQ=60)
                                                     ATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGGTGGATACGC                                                                                               >  SRR19973065.3045203/1‑150 (MQ=60)
                                                        ACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTT                                                                                            <  SRR19973065.1162537/150‑1 (MQ=60)
                                                        ACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTT                                                                                            <  SRR19973065.1162479/150‑1 (MQ=60)
                                                         CCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTC                                                                                           <  SRR19973065.2995459/150‑1 (MQ=60)
                                                           TGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTG                                                                                         <  SRR19973065.2184066/150‑1 (MQ=60)
                                                            GCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGG                                                                                        >  SRR19973065.3490551/1‑150 (MQ=60)
                                                            GCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTAACCGTAGTGATGGATACGCTTTCTGG                                                                                        >  SRR19973065.2768941/1‑150 (MQ=60)
                                                             CTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTGTTAACCTTATTGATTGAAACGCTTTGTGGC                                                                                       >  SRR19973065.2752968/1‑150 (MQ=60)
                                                               ACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTTCCCTTAGTGATGGATACGCTTTCTGGCAT                                                                                     >  SRR19973065.1336970/1‑150 (MQ=60)
                                                               ACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCAT                                                                                     <  SRR19973065.2583792/150‑1 (MQ=60)
                                                                     TGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGT                                                                               <  SRR19973065.1682674/150‑1 (MQ=60)
                                                                      GGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTC                                                                              <  SRR19973065.417258/150‑1 (MQ=60)
                                                                      GGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTC                                                                              <  SRR19973065.2441762/150‑1 (MQ=60)
                                                                      GGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTC                                                                              <  SRR19973065.417318/150‑1 (MQ=60)
                                                                       GTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCG                                                                             <  SRR19973065.2298889/150‑1 (MQ=60)
                                                                       GTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCG                                                                             <  SRR19973065.2298738/150‑1 (MQ=60)
                                                                        TGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGA                                                                            <  SRR19973065.2348959/150‑1 (MQ=60)
                                                                        TGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGA                                                                            <  SRR19973065.1374403/150‑1 (MQ=60)
                                                                          AGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATC                                                                          <  SRR19973065.2772116/150‑1 (MQ=60)
                                                                                GCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTT                                                                    <  SRR19973065.3429358/150‑1 (MQ=60)
                                                                                 CGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTA                                                                   <  SRR19973065.2500262/150‑1 (MQ=60)
                                                                                   CCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATG                                                                 <  SRR19973065.1980287/150‑1 (MQ=60)
                                                                                    CAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGG                                                                <  SRR19973065.1670019/150‑1 (MQ=60)
                                                                                      ACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTG                                                              <  SRR19973065.3272638/150‑1 (MQ=60)
                                                                                            AGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGA                                                        <  SRR19973065.860324/150‑1 (MQ=60)
                                                                                            AGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATAAGCTTTCTGGCATCGGCGTCGATGTGATTTATGGTGAGGTGA                                                        >  SRR19973065.2482156/1‑150 (MQ=60)
                                                                                            AGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTAACCATAGTGATGGATAAGCTTTCTGGCCTCGGCGTCGAagtggtttatggttttgtgt                                                        >  SRR19973065.1618344/1‑130 (MQ=60)
                                                                                              GGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATT                                                      <  SRR19973065.2951943/150‑1 (MQ=60)
                                                                                               GTAATTTGATGGTTGAGATGCTGATTAGGATTATTCTGGGGCACGCTCAGCTACTCGGGATGAAATCCATCGCCGGGCCAGTCGTTTTTCCCTTTTTGATTTATACGCTTTCTGGCATCGGAGTCGATCTGATTTATGGTGAGGTGATTG                                                     <  SRR19973065.1434680/150‑1 (MQ=60)
                                                                                                    TTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGAT                                                <  SRR19973065.2214177/150‑1 (MQ=60)
                                                                                                    TTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGAT                                                <  SRR19973065.513839/150‑1 (MQ=60)
                                                                                                    TTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCGGATTTATGGTGAGGTGATTGCCGAT                                                >  SRR19973065.1429266/1‑150 (MQ=60)
                                                                                                        TGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCC                                            <  SRR19973065.1725681/150‑1 (MQ=60)
                                                                                                            TGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACC                                        <  SRR19973065.3400392/150‑1 (MQ=60)
                                                                                                             gagttgcttatttaGATTATTCAGGGGCACGATCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCG                                       <  SRR19973065.596415/136‑1 (MQ=60)
                                                                                                               GATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCT                                     <  SRR19973065.3005218/150‑1 (MQ=60)
                                                                                                                ATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTGTTACCCATAGTGATGGATACGCTTTCTTGCATCGGCGTCGCTCTGCTTTATTGTGGTGTGATTTCCGAATCCCAACCGCTG                                    >  SRR19973065.2731786/1‑150 (MQ=60)
                                                                                                                 TGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGG                                   <  SRR19973065.711614/150‑1 (MQ=60)
                                                                                                                 TGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGG                                   <  SRR19973065.711675/150‑1 (MQ=60)
                                                                                                                  GCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGA                                  <  SRR19973065.3321806/150‑1 (MQ=60)
                                                                                                                   CTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCGGGAT                                 >  SRR19973065.1778712/1‑150 (MQ=60)
                                                                                                                   CTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCATAGTGATTGATACGCTTTCTTGCATCGGCGTCGATCTGTTTTATGGTGAGGTGATTTCCGATGCCCAAacccgggaa                                 >  SRR19973065.1778747/1‑141 (MQ=60)
                                                                                                                   CTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTAACCGTAGTGTTGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTTCCGAATCCCAACCGCTGGAT                                 >  SRR19973065.1073293/1‑150 (MQ=60)
                                                                                                                         ACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTGTTAACCTTAGTGATTGTTAAGCTTTGTGGCCTCGGCGTCGATAAGATTTATGGTGAGGggatttgcgattgccaaaaccaggattttgtt                           >  SRR19973065.642372/1‑118 (MQ=60)
                                                                                                                          CGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTTACCTTTTTGATGGATACGCTTTCTGGCATCGGCGTCGCTCAGATTTATTGTGAGGTGATTtgcccatcccaaccgcaggatttgaggg                          >  SRR19973065.215200/1‑122 (MQ=60)
                                                                                                                          CGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGG                          <  SRR19973065.2076291/150‑1 (MQ=60)
                                                                                                                          CGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGG                          <  SRR19973065.293732/150‑1 (MQ=60)
                                                                                                                          CGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCGTAGTGATTGATAAGCTTTCTGGCATCGGCGGCCATCTGAGTTTTGGTGAGGTGATTGCCGAATCCCAACCCCTGGAGTTGCTGG                          >  SRR19973065.2862196/1‑150 (MQ=60)
                                                                                                                           GATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTgtttcacgtagtttttgttacgctttcttgcatcgggggggatgggtgttatggtgtggtgttttgcgattgccaaccgcggggtttggttgt                         >  SRR19973065.1211358/1‑57 (MQ=60)
                                                                                                                           GATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGT                         >  SRR19973065.2663914/1‑150 (MQ=60)
                                                                                                                           GATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCGTAGTGATTGATACGCTTTCTTGCATCGGCGTCGATATGATTTTTTGTGTGGTGGTTGGCGATGGCCAACCGCTGGTTTTGGTGGT                         >  SRR19973065.2187265/1‑150 (MQ=60)
                                                                                                                           GATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCGTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGTTTGCCGATGCCCAACCGCTGGATTTGCTGGT                         >  SRR19973065.896261/1‑150 (MQ=60)
                                                                                                                           GATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCGTAGTGATGGATACGCTTTATGGCATCGGCGTCGATGGGATTTATGGTGAGGTGATTGGCGAATCCCAACCGCTGGATTTGCTGGT                         >  SRR19973065.2186695/1‑150 (MQ=60)
                                                                                                                           GATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTGTTACCCgtggtgttggattcgctgtatgggatcggcgtcgtgcggctgtattgggtggtgtttggcgatgcccaaccgctggggttgctggt                         >  SRR19973065.2965146/1‑64 (MQ=60)
                                                                                                                               ATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAAT                     <  SRR19973065.118218/150‑1 (MQ=60)
                                                                                                                                 TCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAG                   <  SRR19973065.2197217/150‑1 (MQ=60)
                                                                                                                                  CAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGT                  <  SRR19973065.2444545/150‑1 (MQ=60)
                                                                                                                                  CAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGT                  <  SRR19973065.1645228/150‑1 (MQ=60)
                                                                                                                                  CAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGT                  <  SRR19973065.411898/150‑1 (MQ=60)
                                                                                                                                  CAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGT                  <  SRR19973065.1645226/150‑1 (MQ=60)
                                                                                                                                   AGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTA                 <  SRR19973065.1643878/150‑1 (MQ=60)
                                                                                                                                     GGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGT               <  SRR19973065.3254878/150‑1 (MQ=60)
                                                                                                                                     GGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGT               <  SRR19973065.3254877/150‑1 (MQ=60)
                                                                                                                                     GGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGT               <  SRR19973065.1894055/150‑1 (MQ=60)
                                                                                                                                      GGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTgtttaccggagtggtggatacgctgtctggcctcggcggcggtcgggtttttggtgggggggttgggggttgcccaccccgggattttgtgggggatatttgtt              >  SRR19973065.311691/1‑46 (MQ=60)
                                                                                                                                      GGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTTACCTTAGTGTTGGATACGCGTTCTTGCATCGGGGTCGATGTGTTTTATGGTtttgtgttttccctttcccaaccccggggttttctgggggatagtagtt              >  SRR19973065.2695754/1‑101 (MQ=60)
                                                                                                                                      GGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTGTTACCCGTAGTTGTGGTTACGCATTGTGGCATCGGCGggggtggggtttttggtgtggggtttggcgatggcccaccgcgggctttgctggtggatatgagtt              >  SRR19973065.729501/1‑84 (MQ=46)
                                                                                                                                      GGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTGTTACCCGTAGTGATGGATAagggttctggcctcggcggggatcggttttttggtgtggtgtttggcgatggccccccgctggctttgctggggtatagtagtt              >  SRR19973065.2695744/1‑66 (MQ=60)
                                                                                                                                        CACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTGTTTCCCTTAGTGATGGTTACGCTTTCTGGCATCGGCGTCGGTCTGGTTTATTGTGTGGTGTTTGGCGATGCCCAACCGCggggtttgggggggatcgggagagca            >  SRR19973065.1344694/1‑124 (MQ=60)
                                                                                                                                           GCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTATTTC         <  SRR19973065.2152780/150‑1 (MQ=60)
                                                                                                                                            CTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTATTTCG        <  SRR19973065.2728738/150‑1 (MQ=60)
                                                                                                                                                GCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTATTTCGCGAT    <  SRR19973065.517987/150‑1 (MQ=60)
                                                                                                                                                  TACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCGTAGTGATGGATAAGCTTTCTGGCAACGGCGTCGAGCTGATTTATGGGGTGGGGATTGCCGATGCCCAACCCCTGGATTTTCTGTTGAATAGTAGTTATTTCGCGATTA  >  SRR19973065.2622065/1‑150 (MQ=60)
                                                                                                                                                  |                                                                                                                                                     
CCAGGTGGGCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCGACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTATTTCGCGATTA  >  CP009273/2139764‑2140059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑