Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F142 I0 R1 859 274.6 8723680 96.4% 8409627 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,139,910 G→T 100% R1055L (CGA→CTA)  yegE → putative diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,139,9100GT97.0% 811.9 / 11.5 232R1055L (CGA→CTA) yegEputative diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base G (2/5);  new base T (113/112);  total (115/117)
Fisher's exact test for biased strand distribution p-value = 4.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 232 total aligned reads displayed.

CCAGGTGGGCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCGACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTATTTCG  >  CP009273/2139764‑2140053
                                                                                                                                                  |                                                                                                                                               
ccAGGTGGGCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACt                                                                                                                                              <  1:100711/150‑1 (MQ=255)
ccAGGTGGGCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACt                                                                                                                                              <  1:1517643/150‑1 (MQ=255)
  aGGTGGGCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCGACTCg                                                                                                                                            <  1:1801911/150‑1 (MQ=255)
     tGGGCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTTCTACTTGATGGTGAGTTATGCGCCAACGTGCTGGGTATTTTGATGGATGTGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCgggt                                                                                                                                         <  2:1404276/150‑2 (MQ=255)
      gggCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGAt                                                                                                                                        <  1:3212918/150‑1 (MQ=255)
      gggCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGAt                                                                                                                                        <  2:4258953/150‑1 (MQ=255)
       ggCCGCCATCAGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCCGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATg                                                                                                                                       >  2:4320145/1‑150 (MQ=255)
           gcgATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTCATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGaaaa                                                                                                                                   >  2:3336230/1‑150 (MQ=255)
            cgATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAAc                                                                                                                                  >  1:1546435/1‑150 (MQ=255)
            cgATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAAc                                                                                                                                  <  2:1760791/150‑1 (MQ=255)
             gATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAAcc                                                                                                                                 >  2:3345646/1‑150 (MQ=255)
              aTCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCa                                                                                                                                >  2:2858110/1‑150 (MQ=255)
              aTCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCa                                                                                                                                <  2:2382804/150‑1 (MQ=255)
              aTCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCa                                                                                                                                >  1:4085174/1‑150 (MQ=255)
                    aaaTCTTCAACTCGCTGAAAGCGAATCTGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCg                                                                                                                          <  2:677807/150‑1 (MQ=255)
                     aaTCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCgg                                                                                                                         >  1:29243/1‑150 (MQ=255)
                         ttCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCa                                                                                                                     <  1:414097/150‑1 (MQ=255)
                            aaCTCGCTGAAAGCGAATATTGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGAAGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCTCCGGGCCAGTc                                                                                                                  >  2:1518628/1‑150 (MQ=255)
                             aCTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCg                                                                                                                 >  2:4041728/1‑150 (MQ=255)
                             aCTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCg                                                                                                                 <  1:1030266/150‑1 (MQ=255)
                              cTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGt                                                                                                                >  1:187641/1‑150 (MQ=255)
                                 gCTGAAATCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGtttt                                                                                                             <  2:1633779/150‑1 (MQ=255)
                                     aaaGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTAccc                                                                                                         >  1:3791330/1‑150 (MQ=255)
                                     aaaGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTGTTAccc                                                                                                         >  1:468921/1‑150 (MQ=255)
                                     aaaGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTGTTAccc                                                                                                         >  1:170266/1‑150 (MQ=255)
                                        gCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTa                                                                                                      <  2:2542495/150‑1 (MQ=255)
                                         cGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAg                                                                                                     <  2:3171522/150‑1 (MQ=255)
                                          gAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGt                                                                                                    <  2:3360083/150‑1 (MQ=255)
                                          gAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGt                                                                                                    >  2:1743737/1‑150 (MQ=255)
                                          gAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGt                                                                                                    <  2:4130244/150‑1 (MQ=255)
                                          gAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCGTAGt                                                                                                    >  1:1593194/1‑150 (MQ=255)
                                           aaTATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTg                                                                                                   <  1:3102041/150‑1 (MQ=255)
                                           aaTATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTg                                                                                                   >  2:1635758/1‑150 (MQ=255)
                                           aaTATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTg                                                                                                   <  1:3976072/150‑1 (MQ=255)
                                               tGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATgg                                                                                               <  2:806369/150‑1 (MQ=255)
                                                  cAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATa                                                                                            <  2:226695/150‑1 (MQ=255)
                                                   aGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTGAGTGATGGATAc                                                                                           >  2:2560768/1‑150 (MQ=255)
                                                      ttACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTGTTTACCTTAGTGATGGATACGcg                                                                                        >  1:759912/1‑149 (MQ=255)
                                                        aCCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCttt                                                                                      <  2:2847878/150‑1 (MQ=255)
                                                         ccTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTc                                                                                     <  2:1898917/150‑1 (MQ=255)
                                                          cTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGTTACGCtttgt                                                                                    >  2:1099938/1‑148 (MQ=255)
                                                          cTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCt                                                                                    >  2:1711591/1‑150 (MQ=255)
                                                          cTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCt                                                                                    <  2:4102292/150‑1 (MQ=255)
                                                           tGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTGTTACCCTTAGTGATGGATACGCTTTCTg                                                                                   >  1:1356975/1‑150 (MQ=255)
                                                             cTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGc                                                                                 <  1:2201408/150‑1 (MQ=255)
                                                               aCTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCAt                                                                               <  2:2486589/150‑1 (MQ=255)
                                                                cTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATc                                                                              <  1:4127591/150‑1 (MQ=255)
                                                                cTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATc                                                                              <  1:435593/150‑1 (MQ=255)
                                                                cTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTGTTACCCTTAGTGATGGATACGCTTTCTGGCATc                                                                              >  1:2460520/1‑150 (MQ=255)
                                                                  tGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCgg                                                                            >  1:603437/1‑150 (MQ=255)
                                                                   gATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGTTGGATACGCTTTCTGGCATCGGc                                                                           >  2:3501844/1‑150 (MQ=255)
                                                                    aTGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCg                                                                          <  2:3411827/150‑1 (MQ=255)
                                                                       gTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCATAGTGATTGATACGCTTTCTGGCATCGgcggcg                                                                       >  1:1489976/1‑150 (MQ=255)
                                                                        tGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGa                                                                      <  1:1880944/150‑1 (MQ=255)
                                                                          aGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATc                                                                    <  1:4041728/150‑1 (MQ=255)
                                                                            ttATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATATg                                                                  >  2:4347796/1‑150 (MQ=255)
                                                                             tATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGa                                                                 <  1:2121732/150‑1 (MQ=255)
                                                                               tGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGAtt                                                               <  1:3524113/150‑1 (MQ=255)
                                                                                gcgcCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGAttt                                                              <  2:1249544/150‑1 (MQ=255)
                                                                                     aaCGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATggt                                                         <  2:2545814/150‑1 (MQ=255)
                                                                                     aaCGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTAACCTTATTGATTGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATggt                                                         >  1:591896/1‑150 (MQ=255)
                                                                                      aCGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATggtg                                                        >  1:323057/1‑150 (MQ=255)
                                                                                        gTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTGTTACCCTTATTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGTTTTATggtgag                                                      >  1:1916032/1‑150 (MQ=255)
                                                                                           cAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATggtgaggtg                                                   <  1:2379472/150‑1 (MQ=255)
                                                                                             gggTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCCGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTCTGGTGAGGTGAt                                                 >  2:4074649/1‑150 (MQ=255)
                                                                                              ggTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCGACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCGTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGAtt                                                >  2:3850098/1‑150 (MQ=255)
                                                                                                tAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGc                                              <  2:100083/150‑1 (MQ=255)
                                                                                                 aaTTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGcc                                             <  2:2062626/150‑1 (MQ=255)
                                                                                                 aaTTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGcc                                             <  1:495526/150‑1 (MQ=255)
                                                                                                 aaTTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGcc                                             >  2:2777472/1‑150 (MQ=255)
                                                                                                     tGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCGTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATg                                         >  2:4206792/1‑150 (MQ=255)
                                                                                                       atggatgAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGTTACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGcc                                       >  2:2943055/1‑150 (MQ=255)
                                                                                                       atggatgAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCGTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGcc                                       >  2:565267/1‑150 (MQ=255)
                                                                                                         ggatgAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATTGTGAGGTGATTGCCGATGCCCa                                     >  2:245886/1‑150 (MQ=255)
                                                                                                         ggatgAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGGCGATCTGAGTTATGGTGAGGTTATTTCCGATGCCCa                                     >  2:3683980/1‑150 (MQ=255)
                                                                                                               gaTGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCt                               <  1:1926219/150‑1 (MQ=255)
                                                                                                                aTGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTg                              <  1:2835617/150‑1 (MQ=255)
                                                                                                                 tGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTAACCGTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATATGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTgg                             >  1:101549/1‑150 (MQ=255)
                                                                                                                 tGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTGTTACCCGTAGTGTTTGTTACGCTTTCTGGCATCGGCGGCGATGTGATTTATGGGGAGGGGATTGCCGATGCCCAAccccagg                             >  1:83207/1‑145 (MQ=255)
                                                                                                                    tGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGAtt                          <  1:880304/150‑1 (MQ=255)
                                                                                                                    tGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGAtt                          <  2:1289661/150‑1 (MQ=255)
                                                                                                                     gATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGAttt                         <  2:3097751/150‑1 (MQ=255)
                                                                                                                      aTTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTg                        <  1:1518628/150‑1 (MQ=255)
                                                                                                                      aTTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCGTAGTGATGGATAAGCTTTATGGCAACGGCGGCGATCTGTTTTATGGTGAGGTGAATGCCGATGCCCAACCGCTGGATTTg                        >  2:1820311/1‑150 (MQ=255)
                                                                                                                        tACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCt                      >  1:1050062/1‑150 (MQ=255)
                                                                                                                        tACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATGTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCt                      >  2:298256/1‑150 (MQ=255)
                                                                                                                         aCGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTg                     <  2:2204818/150‑1 (MQ=255)
                                                                                                                           gATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGt                   <  2:2256743/150‑1 (MQ=255)
                                                                                                                            attattCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATGGGATTTATTGTGAGGTGATTGCCGATTCCCAACCGCTGGATTTGCTGGTg                  >  1:688101/1‑150 (MQ=255)
                                                                                                                             ttattCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGa                 >  2:2039348/1‑150 (MQ=255)
                                                                                                                             ttattCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGa                 >  2:4226280/1‑150 (MQ=255)
                                                                                                                               attCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAAt               <  1:2233058/150‑1 (MQ=255)
                                                                                                                                  cAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTGTTAACCGGTGTGATGGGTACGCGTTCAGGCCTCGGCGTCGATCGGGTTTATGGTGGGGTGGTTGACGCTGCCCCACCGCAGGATTTTCTGGGGTAtagt            >  1:2607861/1‑150 (MQ=255)
                                                                                                                                       gCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTa       <  2:4021744/150‑1 (MQ=255)
                                                                                                                                        cACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTAACCGTAGTGATTGATACGCTTTCTGGCATCGGCGTCGATGTGTTTTATTGTGTGGTGATTTGCGATGCCCAAACCCTGGATTTTCTGGTGTATATTAGTTAt      >  1:4132965/1‑150 (MQ=255)
                                                                                                                                         aCGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGTTTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTAtt     >  2:654819/1‑150 (MQ=255)
                                                                                                                                           gCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTATTTc   <  2:1440451/150‑1 (MQ=255)
                                                                                                                                           gCTCAGCGACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTATTTc   <  1:2978320/150‑1 (MQ=255)
                                                                                                                                            cTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTATTTcg  <  1:3503813/150‑1 (MQ=255)
                                                                                                                                            cTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTATTTcg  <  1:3320310/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
CCAGGTGGGCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCGACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTATTTCG  >  CP009273/2139764‑2140053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGCCAGGTGGGCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCGACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTATTTCGCGAT  >  CP009273/2139761‑2140057
                                                                                                                                                     |                                                                                                                                                   
CAGCCAGGTGGGCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCT                                                                                                                                                     >  SRR19973079.654549/1‑150 (MQ=60)
   CCAGGTGGGCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTCATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACT                                                                                                                                                  >  SRR19973079.2973771/1‑150 (MQ=60)
   CCAGGTGGGCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACT                                                                                                                                                  <  SRR19973079.101608/150‑1 (MQ=60)
   CCAGGTGGGCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACT                                                                                                                                                  <  SRR19973079.1526006/150‑1 (MQ=60)
     AGGTGGGCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCGACTCG                                                                                                                                                <  SRR19973079.1811284/150‑1 (MQ=60)
         GGGCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGAT                                                                                                                                            <  SRR19973079.2619210/150‑1 (MQ=60)
         GGGCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGAT                                                                                                                                            <  SRR19973079.2627369/150‑1 (MQ=60)
         GGGCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGAT                                                                                                                                            <  SRR19973079.3229276/150‑1 (MQ=60)
         GGGCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGAT                                                                                                                                            <  SRR19973079.3229279/150‑1 (MQ=60)
               CGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAAC                                                                                                                                      >  SRR19973079.1554972/1‑150 (MQ=60)
                GATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACC                                                                                                                                     >  SRR19973079.2193898/1‑150 (MQ=60)
                GATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACC                                                                                                                                     >  SRR19973079.3652126/1‑150 (MQ=60)
                GATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCGACTCGGGATGAAAACC                                                                                                                                     >  SRR19973079.483392/1‑150 (MQ=60)
                 ATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCA                                                                                                                                    >  SRR19973079.4105881/1‑150 (MQ=60)
                    TGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGACAACCATCG                                                                                                                                 >  SRR19973079.1654261/1‑150 (MQ=60)
                        AATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGG                                                                                                                             >  SRR19973079.29574/1‑150 (MQ=60)
                        AATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGCGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGG                                                                                                                             >  SRR19973079.2216190/1‑150 (MQ=60)
                            TTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCA                                                                                                                         <  SRR19973079.416526/150‑1 (MQ=60)
                                ACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCG                                                                                                                     <  SRR19973079.1035998/150‑1 (MQ=60)
                                ACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCG                                                                                                                     <  SRR19973079.1036006/150‑1 (MQ=60)
                                 CTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGT                                                                                                                    >  SRR19973079.102430/1‑150 (MQ=60)
                                 CTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGT                                                                                                                    >  SRR19973079.189171/1‑150 (MQ=60)
                                 CTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGT                                                                                                                    >  SRR19973079.189017/1‑150 (MQ=60)
                                 CTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGT                                                                                                                    >  SRR19973079.189010/1‑150 (MQ=60)
                                  TCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTT                                                                                                                   >  SRR19973079.3411875/1‑150 (MQ=60)
                                     CTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTA                                                                                                                >  SRR19973079.3105239/1‑150 (MQ=60)
                                        AAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCC                                                                                                             >  SRR19973079.170477/1‑150 (MQ=60)
                                        AAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCC                                                                                                             >  SRR19973079.3810745/1‑150 (MQ=60)
                                        AAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTGTTACCC                                                                                                             >  SRR19973079.171556/1‑150 (MQ=60)
                                        AAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTGTTACCC                                                                                                             >  SRR19973079.471748/1‑150 (MQ=60)
                                          AGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTT                                                                                                           <  SRR19973079.1526861/150‑1 (MQ=60)
                                          AGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTT                                                                                                           <  SRR19973079.1526853/150‑1 (MQ=60)
                                             GAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGT                                                                                                        <  SRR19973079.565794/150‑1 (MQ=60)
                                             GAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCGTAGT                                                                                                        >  SRR19973079.1601959/1‑150 (MQ=60)
                                              AATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTG                                                                                                       <  SRR19973079.3118058/150‑1 (MQ=60)
                                              AATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTG                                                                                                       <  SRR19973079.923508/150‑1 (MQ=60)
                                              AATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTG                                                                                                       <  SRR19973079.3996066/150‑1 (MQ=60)
                                              AATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTG                                                                                                       <  SRR19973079.1797122/150‑1 (MQ=60)
                                                 ATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTGTTACCCTTAGTGATG                                                                                                    >  SRR19973079.973195/1‑150 (MQ=60)
                                                  TGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTGTTACCCGAAGTGATGG                                                                                                   >  SRR19973079.1552415/1‑150 (MQ=60)
                                                   GGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGA                                                                                                  <  SRR19973079.1514941/150‑1 (MQ=60)
                                                         TTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTGTTTACCTTAGTGATGGATACGCG                                                                                            >  SRR19973079.764095/1‑150 (MQ=60)
                                                            CCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTC                                                                                         <  SRR19973079.3932490/150‑1 (MQ=60)
                                                              TGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTG                                                                                       <  SRR19973079.726020/150‑1 (MQ=60)
                                                              TGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTG                                                                                       <  SRR19973079.2441451/150‑1 (MQ=60)
                                                              TGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTGTTACCCTTAGTGATGGATACGCTTTCTG                                                                                       >  SRR19973079.1364539/1‑150 (MQ=60)
                                                               GCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGG                                                                                      <  SRR19973079.3020469/150‑1 (MQ=60)
                                                                CTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGC                                                                                     <  SRR19973079.2212754/150‑1 (MQ=60)
                                                                CTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTGTTAACCTTAGTGATTGATAAGCTTTCTGGC                                                                                     >  SRR19973079.1490012/1‑150 (MQ=60)
                                                                   CTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATC                                                                                  <  SRR19973079.81502/150‑1 (MQ=60)
                                                                   CTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATC                                                                                  <  SRR19973079.438320/150‑1 (MQ=60)
                                                                   CTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATC                                                                                  <  SRR19973079.438190/150‑1 (MQ=60)
                                                                   CTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATC                                                                                  <  SRR19973079.4148533/150‑1 (MQ=60)
                                                                   CTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTGTTACCCTTAGTGATGGATACGCTTTCTGGCATC                                                                                  >  SRR19973079.2473121/1‑150 (MQ=60)
                                                                     TGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGG                                                                                >  SRR19973079.606948/1‑150 (MQ=60)
                                                                      GATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGC                                                                               <  SRR19973079.4081142/150‑1 (MQ=60)
                                                                        TGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGT                                                                             <  SRR19973079.2767984/150‑1 (MQ=60)
                                                                         GGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTC                                                                            <  SRR19973079.1900743/150‑1 (MQ=60)
                                                                         GGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCATAGTGtttgataagcgttgttgcataggcgtc                                                                            >  SRR19973079.712/1‑123 (MQ=60)
                                                                         GGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCGACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTC                                                                            <  SRR19973079.3995179/150‑1 (MQ=60)
                                                                          GTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCATAGTGATTGATACGCTTTCTGGCATCGGCGGCG                                                                           >  SRR19973079.1498163/1‑150 (MQ=60)
                                                                           TGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGA                                                                          <  SRR19973079.1890946/150‑1 (MQ=60)
                                                                            GAGTTATGCGCCAACGTGCAGGGTAATTTTATGGATGAGATGCTTATTACGATTATTCAGGGGCACGCTCAGCGACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGAT                                                                         <  SRR19973079.112012/150‑1 (MQ=60)
                                                                             AGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATC                                                                        <  SRR19973079.4062160/150‑1 (MQ=60)
                                                                             AGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATC                                                                        <  SRR19973079.3234819/150‑1 (MQ=60)
                                                                             AGTAATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTAACCTAAGTGATtgaaaacagttaatgcatcggcgtcgctc                                                                        >  SRR19973079.978257/1‑121 (MQ=60)
                                                                                TATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGA                                                                     <  SRR19973079.2132712/150‑1 (MQ=60)
                                                                                  TGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATT                                                                   <  SRR19973079.3538860/150‑1 (MQ=60)
                                                                                  TGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATT                                                                   <  SRR19973079.3542207/150‑1 (MQ=60)
                                                                                     GCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATAGGCGTCGATCTGATTTAT                                                                >  SRR19973079.207146/1‑150 (MQ=60)
                                                                                      CCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATG                                                               <  SRR19973079.1029212/150‑1 (MQ=60)
                                                                                        AACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCATAGTGATGGATAAGCTTTCTGGCATCGGCGTCGATCTGATTTATTGT                                                             >  SRR19973079.2361066/1‑150 (MQ=60)
                                                                                        AACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTAACCTTATTGATTGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGT                                                             >  SRR19973079.595359/1‑150 (MQ=60)
                                                                                        AACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTAACCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGTTTTATGGG                                                             >  SRR19973079.597462/1‑150 (MQ=60)
                                                                                         ACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTG                                                            >  SRR19973079.324893/1‑150 (MQ=60)
                                                                                          CGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGA                                                           <  SRR19973079.4122223/150‑1 (MQ=60)
                                                                                           GTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCggctttattgtgtg                                                          >  SRR19973079.1926288/1‑136 (MQ=60)
                                                                                           GTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTGTTACCCTTATTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGTTTTATGGTGAG                                                          >  SRR19973079.1926262/1‑150 (MQ=60)
                                                                                           GTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCGACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTtaccgtagtggttggtaagcgttctggcagcggcgtcgctcgtgttttttgtgtg                                                          >  SRR19973079.746255/1‑95 (MQ=60)
                                                                                           GTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCGACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTtaccgtagtgattggtacgctttattgcataggcgtcgcgagtatttttttggtg                                                          >  SRR19973079.746308/1‑95 (MQ=60)
                                                                                           GTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCGACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTAACCTGAGTGATagataaccatgcaggcaacggcgtcgatctgaataattgttag                                                          >  SRR19973079.746386/1‑107 (MQ=60)
                                                                                              CAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTG                                                       <  SRR19973079.2391787/150‑1 (MQ=60)
                                                                                                 GTTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCGACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATT                                                    <  SRR19973079.2040718/150‑1 (MQ=60)
                                                                                                 GGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATT                                                    <  SRR19973079.4180407/150‑1 (MQ=60)
                                                                                                   TAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTgttaaccatattgattgttaagcgttcttgcaaaggcgtcggtcggttttattttgtggtgatttg                                                  >  SRR19973079.12135/1‑84 (MQ=60)
                                                                                                    AATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCC                                                 <  SRR19973079.498543/150‑1 (MQ=60)
                                                                                                     ATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCATAGTGATGGATACGCTTTCTGGCATCGGCGTCGAGCTGATTTATTGTGTGGTGATTTCCG                                                >  SRR19973079.874965/1‑150 (MQ=60)
                                                                                                          ATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTGTTACCCTTAGTGATGGATAAGCTTTCTTGCATCGGCGTCGATCTGATTTATtgtgttgtgattgacgattac                                           >  SRR19973079.3331443/1‑129 (MQ=60)
                                                                                                          ATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTGTTAACCGTAGTGATGGATACGCATTCTGGCATCGGCGTCGATCgggtttatgtgggggtgttttaccattcc                                           >  SRR19973079.1125366/1‑121 (MQ=60)
                                                                                                               TGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACC                                      <  SRR19973079.1566290/150‑1 (MQ=60)
                                                                                                                GAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACACTAAGTGATGGATACGagttatgtccaccgcgtcgagaaggtttaattttttgtgatttgccaatacacaacc                                     >  SRR19973079.4038248/1‑93 (MQ=60)
                                                                                                                GAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTGTTTACCTTAGTGATGGTTAAGCGTTCTGGCATCGGCGTCGAgagggtttatggtgtggtgatttgccattcccaaccc                                     >  SRR19973079.1836592/1‑113 (MQ=60)
                                                                                                                  GATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCT                                   <  SRR19973079.1936510/150‑1 (MQ=60)
                                                                                                                   ATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTG                                  <  SRR19973079.2850080/150‑1 (MQ=60)
                                                                                                                   ATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTG                                  <  SRR19973079.666898/150‑1 (MQ=60)
                                                                                                                    TGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTAACCGTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATAGGATTTATGGTGAGGTGATTTCCGATGCCCAACCGCTGG                                 >  SRR19973079.102457/1‑150 (MQ=60)
                                                                                                                    TGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTGTTACCCGTAGTGTTTGTTACGCTTTCTGGCATCGGCGGCGATGTGATTTATGGGGAGGGGATTGCCGATGCCCAACCccagg                                 >  SRR19973079.83976/1‑145 (MQ=60)
                                                                                                                      CTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGAT                               <  SRR19973079.3353332/150‑1 (MQ=60)
                                                                                                                       TGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATT                              <  SRR19973079.885399/150‑1 (MQ=60)
                                                                                                                         ATTACGTTTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTG                            <  SRR19973079.1526996/150‑1 (MQ=60)
                                                                                                                         ATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCATAGTGATGGATACGCTTTATGGCATCGGCGTGGAGCTGATTTTTGGTGTGGTGATTTCCGATTCCCAACCcctggttttt                            >  SRR19973079.4029852/1‑140 (MQ=60)
                                                                                                                           TACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAAACGCTGGATTTGCT                          >  SRR19973079.1055850/1‑150 (MQ=60)
                                                                                                                              GATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTAACCTTAGTGATGGATACGCTTTCTGGCATAGGCGTCGATATGATTTATGGTGATGTGATTTCCGATGCCCAAACGCTGGATTTTCTGGT                       >  SRR19973079.2850534/1‑150 (MQ=60)
                                                                                                                               ATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATGGGATTTATTGTGAGGTGATTGCCGATTCCCAACCGCTGGATTTGCTGGTG                      >  SRR19973079.691863/1‑150 (MQ=60)
                                                                                                                               ATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTG                      <  SRR19973079.4369907/150‑1 (MQ=60)
                                                                                                                                TTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTTCCCGTAGTGTTGGATAAGCTTTCTGGCCACGGCGTCGCAATGGTTTATGGTGTGGTGATTGGCGATGCCCCACCCCGGGATTTTCTGGTGT                     >  SRR19973079.4119321/1‑150 (MQ=60)
                                                                                                                                 TATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAA                    <  SRR19973079.2377214/150‑1 (MQ=60)
                                                                                                                                  ATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAAT                   <  SRR19973079.2244617/150‑1 (MQ=60)
                                                                                                                                     CAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTGTTAACCGGTGTGATGGGTACGCGTTCAGGCCTCGGCGTCGATCGGGTTTATGGTGGGGTGGTTGACGCTGCCCCACCGCAGGATTTTCTGGGGTATAGT                >  SRR19973079.2621277/1‑150 (MQ=33)
                                                                                                                                          GCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTA           <  SRR19973079.508732/150‑1 (MQ=60)
                                                                                                                                           CACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTAACCGTAGTGATTGATACGCTTTCTGGCATCGGCGTCGATGTGTTTTATTGTGTGGTGATTTGCGATGCCCAAACCCTGGATTTTCTGGTGTATATTAGTTAT          >  SRR19973079.4153933/1‑150 (MQ=60)
                                                                                                                                           CACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTAACCGTAGTGATGGATAAGCTTTCTTGCATCGGCGTCGATCTGATTTTTGGTGAGGTGATTTCCGATTCCCAACCCCAGGATTTGGTGGGGAAAAGTAGTTAT          >  SRR19973079.4154266/1‑150 (MQ=60)
                                                                                                                                           CACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTGTTACCCGTAGTGATGGATAAGCGTTCTTGCAACCGCGGCGATCTGATTTATGGTGAGGTGATTTCCGATGCCCCACCGCGGGATTTTCTGGTGAATACTAGTTAT          >  SRR19973079.4153419/1‑150 (MQ=60)
                                                                                                                                              GCTCAGCGACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTATTTC       <  SRR19973079.2993645/150‑1 (MQ=60)
                                                                                                                                               CTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTATTTCG      <  SRR19973079.3337281/150‑1 (MQ=60)
                                                                                                                                               CTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTATTTCG      <  SRR19973079.3521829/150‑1 (MQ=60)
                                                                                                                                                  AGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTGTTTCCCGTAGTGTTGGATAAGCGTTATGGCATCGGgggcgatgtgtttttttggggggtgttttgcgctgccccaccccagggtttggtggtggagattagttttttggcgc   >  SRR19973079.379726/1‑73 (MQ=28)
                                                                                                                                                  AGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTGTTACCCGTAGTGATGGGTAAGCTTTATGGCCTCGGCGTCGATgtgttttattgtgtggtgttttgcgcttgcccaccccgggtttttgtgggggatagtttttttttagcga   >  SRR19973079.379977/1‑80 (MQ=53)
                                                                                                                                                   GCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTATTTCGCGAT  <  SRR19973079.3845132/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
CAGCCAGGTGGGCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCGACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTATTTCGCGAT  >  CP009273/2139761‑2140057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑