Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 647,422 G→T 100% intergenic (‑110/‑269) citC ← / → citA [citrate [pro‑3S]‑lyase] ligase/sensory histidine kinase in two‑component regulatory system with CitB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273647,4220GT100.0% 672.4 / NA 207intergenic (‑110/‑269)citC/citA[citrate [pro‑3S]‑lyase] ligase/sensory histidine kinase in two‑component regulatory system with CitB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (98/109);  total (98/109)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 207 total aligned reads displayed.

ACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGAGGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTTTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAATGACTCGCAGG  >  CP009273/647277‑647566
                                                                                                                                                 |                                                                                                                                                
aCGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGt                                                                                                                                                >  2:1579491/1‑148 (MQ=255)
 cGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGta                                                                                                                                               >  2:1661475/1‑148 (MQ=255)
   ttttACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGtata                                                                                                                                             <  1:1170299/148‑1 (MQ=255)
    tttACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAGTGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTatat                                                                                                                                            >  1:2644607/1‑148 (MQ=255)
    tttACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTatat                                                                                                                                            >  1:3292789/1‑148 (MQ=255)
      tACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCt                                                                                                                                          <  1:3191353/148‑1 (MQ=255)
       aCGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCtt                                                                                                                                         <  1:660516/148‑1 (MQ=255)
          ctggTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCttttt                                                                                                                                      <  2:1869787/146‑1 (MQ=255)
          cgGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCttttt                                                                                                                                      <  2:1255173/148‑1 (MQ=255)
          cgGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCttttt                                                                                                                                      <  2:152034/148‑1 (MQ=255)
           gggTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTtc                                                                                                                                     <  1:2299633/148‑1 (MQ=255)
             gTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTtctc                                                                                                                                   >  2:3198397/1‑148 (MQ=255)
              tGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTcc                                                                                                                                  <  1:924313/148‑1 (MQ=255)
               gAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCt                                                                                                                                 <  2:735306/148‑1 (MQ=255)
               gAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCt                                                                                                                                 <  1:421649/148‑1 (MQ=255)
                aaaaTATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCtt                                                                                                                                >  1:1397307/1‑148 (MQ=255)
                   atatCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCg                                                                                                                             >  2:1371929/1‑148 (MQ=255)
                    tatCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGc                                                                                                                            <  1:1533733/148‑1 (MQ=255)
                     atCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCt                                                                                                                           >  2:3009867/1‑148 (MQ=255)
                     atCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCt                                                                                                                           >  2:938166/1‑148 (MQ=255)
                      tCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCta                                                                                                                          >  1:1674784/1‑148 (MQ=255)
                        aTTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCtata                                                                                                                        >  2:2969815/1‑148 (MQ=255)
                         ttGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATAc                                                                                                                       >  1:1425001/1‑148 (MQ=255)
                         ttGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATAc                                                                                                                       >  1:3256833/1‑148 (MQ=255)
                              gAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCt                                                                                                                  <  1:2480038/148‑1 (MQ=255)
                              gAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCt                                                                                                                  >  1:2019587/1‑148 (MQ=255)
                               aaCATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTa                                                                                                                 >  2:886157/1‑148 (MQ=255)
                               aaCATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTa                                                                                                                 <  2:2129443/148‑1 (MQ=255)
                                aCATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTaa                                                                                                                >  1:1032931/1‑148 (MQ=255)
                                aCATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTaa                                                                                                                >  1:1343288/1‑148 (MQ=255)
                                 cATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAg                                                                                                               <  1:694093/148‑1 (MQ=255)
                                  ataataAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAgg                                                                                                              <  2:1217826/148‑1 (MQ=255)
                                    aataaataGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAg                                                                                                            >  2:1793799/1‑148 (MQ=255)
                                    aataaataGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAg                                                                                                            >  1:179027/1‑148 (MQ=255)
                                      taaataGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTa                                                                                                          <  1:559161/148‑1 (MQ=255)
                                       aaataGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGATAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTAt                                                                                                         <  1:262183/148‑1 (MQ=255)
                                            gTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCgg                                                                                                    <  2:467513/148‑1 (MQ=255)
                                             tATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATTTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGc                                                                                                   <  2:244055/148‑1 (MQ=255)
                                              aTCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCg                                                                                                  <  1:3287392/148‑1 (MQ=255)
                                                ccTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTg                                                                                                >  2:3119639/1‑148 (MQ=255)
                                                  tGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGaa                                                                                              <  2:3299084/148‑1 (MQ=255)
                                                  tGAAGGTGCATGTTGTTATCGATGTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGaa                                                                                              <  2:3298960/148‑1 (MQ=255)
                                                   gAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGaaa                                                                                             >  2:882179/1‑148 (MQ=255)
                                                     aGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAAtt                                                                                           <  2:3275357/148‑1 (MQ=255)
                                                      ggTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAAttt                                                                                          >  1:1391912/1‑148 (MQ=255)
                                                       gTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAAtttt                                                                                         >  1:1755693/1‑148 (MQ=255)
                                                       gTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAAtttt                                                                                         >  1:3021806/1‑148 (MQ=255)
                                                       gTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAAtttt                                                                                         <  1:2192824/148‑1 (MQ=255)
                                                       gTGCATGTTGTTATCGATTTGCAACGAATGTTGGTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGTAAtttt                                                                                         >  1:3021687/1‑148 (MQ=255)
                                                          cATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGat                                                                                      <  1:256911/148‑1 (MQ=255)
                                                           aTGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGatt                                                                                     <  2:3253691/148‑1 (MQ=255)
                                                           aTGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGatt                                                                                     >  2:1013529/1‑148 (MQ=255)
                                                            tgttgtTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGattt                                                                                    >  1:1675268/1‑148 (MQ=255)
                                                            tgttgtTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGattt                                                                                    <  1:2404390/148‑1 (MQ=255)
                                                               tgttATCGATGTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGatttatt                                                                                 <  1:3274494/148‑1 (MQ=255)
                                                                   aTCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTcac                                                                             <  2:1674784/148‑1 (MQ=255)
                                                                        tttGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATaga                                                                        <  2:2341415/148‑1 (MQ=255)
                                                                        tttGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATaga                                                                        >  2:548265/1‑148 (MQ=255)
                                                                         ttGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATagag                                                                       <  2:1032931/148‑1 (MQ=255)
                                                                          tGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGt                                                                      <  1:2914301/148‑1 (MQ=255)
                                                                          tGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGt                                                                      <  2:223946/148‑1 (MQ=255)
                                                                              aCGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGt                                                                  <  2:51339/148‑1 (MQ=255)
                                                                               cGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTg                                                                 >  2:476763/1‑148 (MQ=255)
                                                                                 aaTGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGt                                                               <  2:2726873/148‑1 (MQ=255)
                                                                                  aTGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGtt                                                              >  1:2867879/1‑148 (MQ=255)
                                                                                    gttgttCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGtttt                                                            >  1:1344657/1‑148 (MQ=255)
                                                                                    gttgttCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGtttt                                                            >  1:3030822/1‑148 (MQ=255)
                                                                                      tgttCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGttttct                                                          >  2:149517/1‑146 (MQ=255)
                                                                                        ttCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTtctat                                                        <  1:2685302/148‑1 (MQ=255)
                                                                                        ttCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTtctat                                                        <  2:2975797/148‑1 (MQ=255)
                                                                                          cAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTtctattt                                                      >  2:2195125/1‑148 (MQ=255)
                                                                                             tGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTtctatttatt                                                   <  2:1109625/148‑1 (MQ=255)
                                                                                              gTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTtctatttattt                                                  >  2:377921/1‑148 (MQ=255)
                                                                                                 gCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAAt                                               <  1:3113582/148‑1 (MQ=255)
                                                                                                      cTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGAttt                                          >  1:2748201/1‑148 (MQ=255)
                                                                                                       tGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGAtttt                                         >  2:1506964/1‑148 (MQ=255)
                                                                                                            aCCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGtt                                    <  1:2190050/148‑1 (MQ=255)
                                                                                                             ccTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGttt                                   <  2:2949995/148‑1 (MQ=255)
                                                                                                              cTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGtttt                                  <  1:843859/148‑1 (MQ=255)
                                                                                                              cTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGtttt                                  >  2:648801/1‑148 (MQ=255)
                                                                                                                tttATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGtttttt                                >  1:1254098/1‑148 (MQ=255)
                                                                                                                 ttATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTtta                               <  1:452782/148‑1 (MQ=255)
                                                                                                                 ttATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTtta                               <  2:576434/148‑1 (MQ=255)
                                                                                                                  tATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTttaa                              <  2:893313/148‑1 (MQ=255)
                                                                                                                   aTTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTttaat                             <  2:1615855/148‑1 (MQ=255)
                                                                                                                    ttttCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTttaatt                            <  2:704632/148‑1 (MQ=255)
                                                                                                                       tCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTtaattaat                         >  2:2910487/1‑148 (MQ=255)
                                                                                                                        cACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATg                        >  1:442777/1‑148 (MQ=255)
                                                                                                                         aCTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGt                       <  1:413895/148‑1 (MQ=255)
                                                                                                                          cTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTtaattaat                         >  2:178480/1‑145 (MQ=255)
                                                                                                                          cTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTa                      >  1:1062099/1‑148 (MQ=255)
                                                                                                                           ttGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTaa                     >  1:815590/1‑148 (MQ=255)
                                                                                                                            tGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAAt                    >  2:1991957/1‑148 (MQ=255)
                                                                                                                              ggAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTa                  >  1:2234719/1‑148 (MQ=255)
                                                                                                                                  aaaGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGaa              >  2:860154/1‑148 (MQ=255)
                                                                                                                                    aGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAAt            >  1:2271882/1‑148 (MQ=255)
                                                                                                                                     gggggTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAATg           <  2:1343567/148‑1 (MQ=255)
                                                                                                                                      ggggTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAATGa          <  2:2644607/148‑1 (MQ=255)
                                                                                                                                            aTCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAATGACTCGCa    >  1:2814993/1‑148 (MQ=255)
                                                                                                                                              cGATGTAGATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAATGACTCGCAgg  >  1:790392/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
ACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGAGGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTTTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAATGACTCGCAGG  >  CP009273/647277‑647566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGAGGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTTTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAATGACTCGCAGGTT  >  CP009273/647273‑647568
                                                                                                                                                     |                                                                                                                                                  
CTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGAT                                                                                                                                                    <  SRR19973070.2392121/150‑1 (MQ=60)
  GAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGT                                                                                                                                                  >  SRR19973070.2356950/1‑150 (MQ=60)
    ACGTTTTACGCGGGTGAAAATATCATTGCAGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTAT                                                                                                                                                <  SRR19973070.1408887/150‑1 (MQ=60)
      GTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAGTGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATAT                                                                                                                                              >  SRR19973070.2661832/1‑150 (MQ=60)
      GTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATAT                                                                                                                                              >  SRR19973070.3313907/1‑150 (MQ=60)
       TTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATC                                                                                                                                             <  SRR19973070.1178493/150‑1 (MQ=60)
       TTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATC                                                                                                                                             <  SRR19973070.3315666/150‑1 (MQ=60)
       TTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATC                                                                                                                                             >  SRR19973070.2274735/1‑150 (MQ=60)
        TTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCT                                                                                                                                            <  SRR19973070.1234357/150‑1 (MQ=60)
        TTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCT                                                                                                                                            <  SRR19973070.2954020/150‑1 (MQ=60)
          TACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTT                                                                                                                                          <  SRR19973070.3212001/150‑1 (MQ=60)
           ACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTT                                                                                                                                         <  SRR19973070.665219/150‑1 (MQ=60)
           ACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTT                                                                                                                                         <  SRR19973070.666129/150‑1 (MQ=60)
             GCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTC                                                                                                                                       <  SRR19973070.1403527/150‑1 (MQ=60)
             GCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTC                                                                                                                                       <  SRR19973070.80628/150‑1 (MQ=60)
              CGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCT                                                                                                                                      >  SRR19973070.2774524/1‑150 (MQ=60)
               GGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTC                                                                                                                                     <  SRR19973070.2314910/150‑1 (MQ=60)
               GGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTC                                                                                                                                     <  SRR19973070.2314931/150‑1 (MQ=60)
                GGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCTATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCC                                                                                                                                    >  SRR19973070.2447454/1‑150 (MQ=60)
                  TGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTT                                                                                                                                  <  SRR19973070.930899/150‑1 (MQ=60)
                  TGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTT                                                                                                                                  >  SRR19973070.1406716/1‑150 (MQ=60)
                   GAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTT                                                                                                                                 <  SRR19973070.424728/150‑1 (MQ=60)
                   GAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTT                                                                                                                                 <  SRR19973070.424723/150‑1 (MQ=60)
                   GAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTT                                                                                                                                 <  SRR19973070.424667/150‑1 (MQ=60)
                     AAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCG                                                                                                                               <  SRR19973070.1518490/150‑1 (MQ=60)
                       ATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCT                                                                                                                             >  SRR19973070.2910016/1‑150 (MQ=60)
                        tgtgctcttccgatcTAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTA                                                                                                                            <  SRR19973070.2218114/135‑1 (MQ=60)
                        TATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTA                                                                                                                            >  SRR19973070.1686255/1‑150 (MQ=60)
                        TATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTA                                                                                                                            <  SRR19973070.1544501/150‑1 (MQ=60)
                           CATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATAC                                                                                                                         >  SRR19973070.1434809/1‑150 (MQ=60)
                           CATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATAC                                                                                                                         >  SRR19973070.3277807/1‑150 (MQ=60)
                            ATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACA                                                                                                                        >  SRR19973070.1379905/1‑150 (MQ=60)
                                CCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCT                                                                                                                    >  SRR19973070.2033245/1‑150 (MQ=60)
                                 CGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTA                                                                                                                   >  SRR19973070.899628/1‑150 (MQ=60)
                                  GAACATAATATATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAA                                                                                                                  <  SRR19973070.2496621/150‑1 (MQ=60)
                                  GAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAA                                                                                                                  <  SRR19973070.2496179/150‑1 (MQ=60)
                                  GAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAA                                                                                                                  >  SRR19973070.1352789/1‑150 (MQ=60)
                                  GAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAA                                                                                                                  >  SRR19973070.1040237/1‑150 (MQ=60)
                                  GAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAA                                                                                                                  >  SRR19973070.1352509/1‑150 (MQ=60)
                                   AACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAG                                                                                                                 >  SRR19973070.1627099/1‑150 (MQ=60)
                                     CATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGA                                                                                                               <  SRR19973070.699044/150‑1 (MQ=60)
                                      ATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAG                                                                                                              >  SRR19973070.180444/1‑150 (MQ=60)
                                       TAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGT                                                                                                             <  SRR19973070.309442/150‑1 (MQ=60)
                                          TAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATT                                                                                                          <  SRR19973070.562836/150‑1 (MQ=60)
                                           AAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGATAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTT                                                                                                         <  SRR19973070.263930/150‑1 (MQ=60)
                                                  ATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTG                                                                                                  <  SRR19973070.3308930/150‑1 (MQ=60)
                                                  ATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTG                                                                                                  <  SRR19973070.3308494/150‑1 (MQ=60)
                                                     CTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAA                                                                                               >  SRR19973070.843929/1‑150 (MQ=60)
                                                        AAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTT                                                                                            >  SRR19973070.1401304/1‑150 (MQ=60)
                                                         AGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTT                                                                                           >  SRR19973070.3041040/1‑150 (MQ=60)
                                                         AGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTT                                                                                           >  SRR19973070.3041060/1‑150 (MQ=60)
                                                         AGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTT                                                                                           >  SRR19973070.1767854/1‑150 (MQ=60)
                                                         AGGTGCATGTTGTTATCGATTTGCAACGAATGTTGGTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGTAATTTT                                                                                           >  SRR19973070.3040941/1‑150 (MQ=60)
                                                           GTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGA                                                                                         >  SRR19973070.527653/1‑150 (MQ=60)
                                                           GTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGA                                                                                         >  SRR19973070.709652/1‑150 (MQ=60)
                                                           GTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGA                                                                                         <  SRR19973070.2207303/150‑1 (MQ=60)
                                                           GTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGA                                                                                         <  SRR19973070.2207190/150‑1 (MQ=60)
                                                            TGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGAT                                                                                        >  SRR19973070.3195242/1‑150 (MQ=60)
                                                            TGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGAT                                                                                        >  SRR19973070.168859/1‑150 (MQ=60)
                                                              CATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTT                                                                                      <  SRR19973070.1279353/150‑1 (MQ=60)
                                                              CATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTT                                                                                      <  SRR19973070.258642/150‑1 (MQ=60)
                                                              CATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTT                                                                                      >  SRR19973070.1686739/1‑150 (MQ=60)
                                                                TGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTAT                                                                                    <  SRR19973070.2420249/150‑1 (MQ=60)
                                                                   TGTTATCGATTTTCAACTAATGTTGTTCAATGTTTCAAACTGATAAACTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTC                                                                                 <  SRR19973070.2705892/150‑1 (MQ=60)
                                                                   TGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTC                                                                                 <  SRR19973070.3295134/150‑1 (MQ=60)
                                                                   TGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTC                                                                                 <  SRR19973070.3295564/150‑1 (MQ=60)
                                                                   TGTTATCGATGTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTC                                                                                 <  SRR19973070.3295543/150‑1 (MQ=60)
                                                                      TATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACA                                                                              >  SRR19973070.624654/1‑150 (MQ=60)
                                                                            TTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGT                                                                        <  SRR19973070.1806191/150‑1 (MQ=60)
                                                                              TGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTA                                                                      <  SRR19973070.2933130/150‑1 (MQ=60)
                                                                              TGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTA                                                                      <  SRR19973070.2933112/150‑1 (MQ=60)
                                                                                   CGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGT                                                                 <  SRR19973070.212135/150‑1 (MQ=60)
                                                                                    GAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTT                                                                >  SRR19973070.2886458/1‑150 (MQ=60)
                                                                                    GAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTT                                                                >  SRR19973070.2886449/1‑150 (MQ=60)
                                                                                     AATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTT                                                               <  SRR19973070.935793/150‑1 (MQ=60)
                                                                                      ATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTT                                                              >  SRR19973070.3050109/1‑150 (MQ=60)
                                                                                      ATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTT                                                              >  SRR19973070.1353885/1‑150 (MQ=60)
                                                                                       TGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGAGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTC                                                             <  SRR19973070.3046832/150‑1 (MQ=60)
                                                                                         TTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTA                                                           <  SRR19973070.2593310/150‑1 (MQ=60)
                                                                                            TTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTT                                                        >  SRR19973070.451728/1‑150 (MQ=60)
                                                                                            TTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTT                                                        <  SRR19973070.2702714/150‑1 (MQ=60)
                                                                                            TTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTT                                                        <  SRR19973070.2702003/150‑1 (MQ=60)
                                                                                                    TGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATG                                                <  SRR19973070.2654069/150‑1 (MQ=60)
                                                                                                     GCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGA                                               <  SRR19973070.3133675/150‑1 (MQ=60)
                                                                                                        AACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTT                                            >  SRR19973070.2765861/1‑150 (MQ=60)
                                                                                                              TAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTT                                      >  SRR19973070.2089775/1‑150 (MQ=60)
                                                                                                                ACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTT                                    <  SRR19973070.2204513/150‑1 (MQ=60)
                                                                                                                  CTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTT                                  <  SRR19973070.849593/150‑1 (MQ=60)
                                                                                                                  CTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTT                                  >  SRR19973070.1262898/1‑150 (MQ=60)
                                                                                                                     TTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAAT                               <  SRR19973070.456040/150‑1 (MQ=60)
                                                                                                                      TATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATT                              >  SRR19973070.175274/1‑150 (MQ=60)
                                                                                                                          TTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATG                          >  SRR19973070.445979/1‑150 (MQ=60)
                                                                                                                           TCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGT                         <  SRR19973070.179896/150‑1 (MQ=60)
                                                                                                                            CACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTA                        >  SRR19973070.1069546/1‑150 (MQ=60)
                                                                                                                            CACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTA                        >  SRR19973070.1069594/1‑150 (MQ=60)
                                                                                                                            CACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATATTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTA                        >  SRR19973070.1069479/1‑150 (MQ=60)
                                                                                                                             ACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAA                       >  SRR19973070.821170/1‑150 (MQ=60)
                                                                                                                             ACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAA                       <  SRR19973070.416917/150‑1 (MQ=60)
                                                                                                                                TGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTA                    >  SRR19973070.2249453/1‑150 (MQ=60)
                                                                                                                                      AAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAAT              >  SRR19973070.2286968/1‑150 (MQ=60)
                                                                                                                                       AAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAATG             >  SRR19973070.3212829/1‑150 (MQ=60)
                                                                                                                                             GTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTAAGAAATtactaga       >  SRR19973070.1751203/1‑143 (MQ=60)
                                                                                                                                              TGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAATGACTCGCA      >  SRR19973070.2833194/1‑150 (MQ=60)
                                                                                                                                                ATCGATGTAGATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAATGACTCGCAGG    >  SRR19973070.795871/1‑150 (MQ=60)
                                                                                                                                                 TCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAATGACTCGCAGGT   <  SRR19973070.557673/150‑1 (MQ=60)
                                                                                                                                                  CGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAATGACTCGCAGGTT  >  SRR19973070.78250/1‑150 (MQ=60)
                                                                                                                                                  CGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAATGACTCGCAGGTT  >  SRR19973070.484261/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                  
CTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGAGGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTTTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAATGACTCGCAGGTT  >  CP009273/647273‑647568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑