Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F159 I0 R1 879 305.9 9588012 96.6% 9262019 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 647,422 G→T 100% intergenic (‑110/‑269) citC ← / → citA [citrate [pro‑3S]‑lyase] ligase/sensory histidine kinase in two‑component regulatory system with CitB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273647,4220GT96.2% 515.4 / 10.7 156intergenic (‑110/‑269)citC/citA[citrate [pro‑3S]‑lyase] ligase/sensory histidine kinase in two‑component regulatory system with CitB
Reads supporting (aligned to +/- strand):  ref base G (4/2);  new base T (63/87);  total (67/89)
Fisher's exact test for biased strand distribution p-value = 4.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 156 total aligned reads displayed.

GAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGAGGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTTTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAATGACTCGCA  >  CP009273/647275‑647564
                                                                                                                                                   |                                                                                                                                              
gAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGAGGt                                                                                                                                              <  1:1279353/150‑1 (MQ=255)
gAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGAGGt                                                                                                                                              >  2:935572/1‑150 (MQ=255)
 aaCGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGta                                                                                                                                             >  2:2269030/1‑150 (MQ=255)
 aaCGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGta                                                                                                                                             <  1:2457995/150‑1 (MQ=255)
    gTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTatat                                                                                                                                          >  2:723117/1‑150 (MQ=255)
    gTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTatat                                                                                                                                          <  1:1782055/150‑1 (MQ=255)
    gTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTatat                                                                                                                                          >  2:3052049/1‑150 (MQ=255)
       ttACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCtt                                                                                                                                       <  2:2463255/150‑1 (MQ=255)
        tACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCttt                                                                                                                                      <  1:3578155/150‑1 (MQ=255)
          cgcgGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATtttttt                                                                                                                                    <  1:272975/150‑1 (MQ=255)
           gcgGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTtc                                                                                                                                   <  1:4185040/150‑1 (MQ=255)
             gggTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTtctc                                                                                                                                 >  2:4776892/1‑150 (MQ=255)
             gggTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTtctc                                                                                                                                 >  2:4776877/1‑150 (MQ=255)
             gggTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTtctc                                                                                                                                 >  2:4020188/1‑150 (MQ=255)
              ggTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTcc                                                                                                                                <  1:656199/150‑1 (MQ=255)
                tGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCtt                                                                                                                              <  2:2060823/150‑1 (MQ=255)
                  aaaaTATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGATAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTc                                                                                                                            >  2:1921943/1‑150 (MQ=255)
                  aaaaTATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTCTATCTTTTTCTCCTTTc                                                                                                                            >  1:4186077/1‑150 (MQ=255)
                      tatCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCta                                                                                                                        <  1:820406/150‑1 (MQ=255)
                      tatCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCta                                                                                                                        <  2:1675497/150‑1 (MQ=255)
                      tatCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCta                                                                                                                        <  1:867032/150‑1 (MQ=255)
                      tatCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCta                                                                                                                        <  1:3370220/150‑1 (MQ=255)
                       atCATGGCCGAACATACTAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCtat                                                                                                                       <  2:3396074/150‑1 (MQ=255)
                        tCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATAGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCtata                                                                                                                      <  1:4434358/150‑1 (MQ=255)
                                gAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTaa                                                                                                              >  2:3915310/1‑150 (MQ=255)
                                 aaCATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATTTTTTTCTCCTTTCGCTATACATCCTAAg                                                                                                             >  1:2693194/1‑150 (MQ=255)
                                 aaCATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAg                                                                                                             >  1:3318309/1‑150 (MQ=255)
                                 aaCATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAg                                                                                                             >  2:707204/1‑150 (MQ=255)
                                 aaCATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAg                                                                                                             >  1:889961/1‑150 (MQ=255)
                                    ataataAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAg                                                                                                          <  1:2403056/150‑1 (MQ=255)
                                     taataaATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGt                                                                                                         >  2:4202212/1‑150 (MQ=255)
                                     taataaATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGt                                                                                                         >  2:1976192/1‑150 (MQ=255)
                                       ataaataGTATCCTGCAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTGTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTAt                                                                                                       <  2:4625418/150‑1 (MQ=255)
                                       ataaataGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTAt                                                                                                       <  2:2143153/150‑1 (MQ=255)
                                       ataaataGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTAt                                                                                                       <  2:2143146/150‑1 (MQ=255)
                                       ataaataGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTAt                                                                                                       <  1:4696378/150‑1 (MQ=255)
                                       ataaataGTATCCTGAAGGTGCATGATGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTAt                                                                                                       >  2:1059190/1‑150 (MQ=255)
                                         aaataGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTAttt                                                                                                     <  2:4030604/150‑1 (MQ=255)
                                           ataGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCg                                                                                                   >  2:3510494/1‑150 (MQ=255)
                                           ataGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCg                                                                                                   <  1:57112/150‑1 (MQ=255)
                                           ataGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCg                                                                                                   <  1:286957/150‑1 (MQ=255)
                                                 tCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGa                                                                                             <  1:3509370/150‑1 (MQ=255)
                                                 tCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGa                                                                                             <  1:3449120/150‑1 (MQ=255)
                                                  ccTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGaa                                                                                            >  2:1794367/1‑150 (MQ=255)
                                                   cTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGaaa                                                                                           <  2:3464683/150‑1 (MQ=255)
                                                    tGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAAt                                                                                          <  1:570135/150‑1 (MQ=255)
                                                       aGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAAtttt                                                                                       <  2:3914079/150‑1 (MQ=255)
                                                               gttgttATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGatttatt                                                                               >  2:205158/1‑150 (MQ=255)
                                                                ttgttATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGatttattt                                                                              >  2:1493432/1‑150 (MQ=255)
                                                                       cGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTCTTTCGGCGTGAAAGTTTGATTTATTTCACATag                                                                       >  1:3029928/1‑150 (MQ=255)
                                                                         atgtGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATagag                                                                     <  1:3242545/147‑1 (MQ=255)
                                                                         aTTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATTTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATagag                                                                     >  1:1342363/1‑150 (MQ=255)
                                                                         aTTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATTTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATagag                                                                     >  1:1341571/1‑150 (MQ=255)
                                                                            tGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTa                                                                  <  1:2108561/150‑1 (MQ=255)
                                                                            tGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTa                                                                  <  1:2575149/150‑1 (MQ=255)
                                                                            tGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTa                                                                  <  1:180833/150‑1 (MQ=255)
                                                                               aaCGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTg                                                               <  2:2245104/150‑1 (MQ=255)
                                                                                  gAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGtt                                                            <  2:2206613/150‑1 (MQ=255)
                                                                                  gAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGtt                                                            <  1:1407393/150‑1 (MQ=255)
                                                                                         gttCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTtctatt                                                     <  1:1945743/150‑1 (MQ=255)
                                                                                         gttCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTtctatt                                                     <  2:1661334/150‑1 (MQ=255)
                                                                                           tCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTtctattta                                                   >  2:4097410/1‑150 (MQ=255)
                                                                                           tCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTtctattta                                                   >  2:278799/1‑150 (MQ=255)
                                                                                             aaTGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTtctatttatt                                                 >  1:4472394/1‑150 (MQ=255)
                                                                                             aaTGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTtctatttatt                                                 <  1:4471480/150‑1 (MQ=255)
                                                                                              aTGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTtctatttattt                                                <  1:2355208/150‑1 (MQ=255)
                                                                                               tGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGAGGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTtttatttattta                                               <  2:4422989/150‑1 (MQ=255)
                                                                                                gTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTaa                                              >  2:2301879/1‑150 (MQ=255)
                                                                                                  tGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTGTATTTATTTTATg                                            >  2:3812648/1‑150 (MQ=255)
                                                                                                       aCTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGAtttt                                       <  1:2991303/150‑1 (MQ=255)
                                                                                                         tGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATGTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTATTGGTTTTCTATTTATTTAATGATTTTaa                                     >  1:962623/1‑150 (MQ=255)
                                                                                                          gATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAg                                    >  1:2509405/1‑150 (MQ=255)
                                                                                                          gATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAg                                    >  2:346091/1‑150 (MQ=255)
                                                                                                          gATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGAGTTTAAg                                    >  2:1665841/1‑150 (MQ=255)
                                                                                                           aTAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGt                                   >  1:3511996/1‑150 (MQ=255)
                                                                                                           aTAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGt                                   >  1:314090/1‑150 (MQ=255)
                                                                                                            tAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATTTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGtt                                  >  1:431487/1‑150 (MQ=255)
                                                                                                            tAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATTTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGtt                                  >  1:431503/1‑150 (MQ=255)
                                                                                                            tAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATTTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGtt                                  >  1:2825277/1‑150 (MQ=255)
                                                                                                               ccTTTTATTGTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGttttt                               <  1:4552860/150‑1 (MQ=255)
                                                                                                                  tttATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTttaa                            <  2:463449/150‑1 (MQ=255)
                                                                                                                  tttATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTttaa                            <  1:2459703/150‑1 (MQ=255)
                                                                                                                   ttATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTttaat                           >  1:2424857/1‑150 (MQ=255)
                                                                                                                   ttATTTTCACTTGGGAGAAAGGGGGTGATCGAGGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTTTATTTATTTAATGATTTTAAGTTTTttaat                           >  1:938745/1‑150 (MQ=255)
                                                                                                                       tttCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTtaattaat                       <  1:294246/150‑1 (MQ=255)
                                                                                                                        ttCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATg                      <  1:1976192/150‑1 (MQ=255)
                                                                                                                        ttCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATg                      <  1:4365317/150‑1 (MQ=255)
                                                                                                                        ttCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATg                      <  1:4202212/150‑1 (MQ=255)
                                                                                                                         tCACTTGGGAGAAAGGGGGTGATCGATGTATATTTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTtaattaattt                     >  1:4131210/1‑148 (MQ=255)
                                                                                                                         tCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGt                     >  2:410663/1‑150 (MQ=255)
                                                                                                                         tCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGt                     <  1:4444273/150‑1 (MQ=255)
                                                                                                                         tCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGt                     <  2:1836245/150‑1 (MQ=255)
                                                                                                                          cACTTGGGATAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTa                    <  2:3809740/150‑1 (MQ=255)
                                                                                                                            cTTGGGAGAAAGGGGGTGATCGATGTATATTTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAAt                  <  2:2825277/150‑1 (MQ=255)
                                                                                                                            cTTGGGAGAAAGGGGGTGATCGATGTATATTTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAAt                  <  2:431487/150‑1 (MQ=255)
                                                                                                                            cTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAAt                  >  1:1406580/1‑150 (MQ=255)
                                                                                                                                ggAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACg              <  1:3915310/150‑1 (MQ=255)
                                                                                                                                        ggggTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAATGACt      <  1:1114500/150‑1 (MQ=255)
                                                                                                                                            tGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGt                     >  1:3826634/1‑131 (MQ=255)
                                                                                                                                            tGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAATGACTCGCa  <  1:3408508/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
GAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGAGGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTTTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAATGACTCGCA  >  CP009273/647275‑647564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGAGGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTTTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAATGACTCGCAGGTTTAA  >  CP009273/647274‑647571
                                                                                                                                                    |                                                                                                                                                     
TGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATG                                                                                                                                                      >  SRR19973078.24414/1‑150 (MQ=60)
 GAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGAGGT                                                                                                                                                     <  SRR19973078.1287332/150‑1 (MQ=60)
  AACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTA                                                                                                                                                    <  SRR19973078.2472085/150‑1 (MQ=60)
  AACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTA                                                                                                                                                    <  SRR19973078.2472069/150‑1 (MQ=60)
     GTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATAT                                                                                                                                                 <  SRR19973078.573986/150‑1 (MQ=60)
     GTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATAT                                                                                                                                                 <  SRR19973078.1792862/150‑1 (MQ=60)
         TACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTT                                                                                                                                             <  SRR19973078.3598268/150‑1 (MQ=60)
           CGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATTTTTTT                                                                                                                                           <  SRR19973078.274933/150‑1 (MQ=60)
            GCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTC                                                                                                                                          <  SRR19973078.4209313/150‑1 (MQ=60)
            GCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTC                                                                                                                                          <  SRR19973078.4208925/150‑1 (MQ=60)
               GGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCC                                                                                                                                       <  SRR19973078.660371/150‑1 (MQ=60)
                  GAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTT                                                                                                                                    <  SRR19973078.4249566/150‑1 (MQ=60)
                   AAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTCTATCTTTTTCTCCTTTC                                                                                                                                   >  SRR19973078.4209966/1‑150 (MQ=60)
                       TATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTA                                                                                                                               <  SRR19973078.872414/150‑1 (MQ=60)
                       TATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTA                                                                                                                               <  SRR19973078.872341/150‑1 (MQ=60)
                       TATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTA                                                                                                                               <  SRR19973078.825225/150‑1 (MQ=60)
                       TATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTA                                                                                                                               <  SRR19973078.3389515/150‑1 (MQ=60)
                         TCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATAGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATA                                                                                                                             <  SRR19973078.4459369/150‑1 (MQ=60)
                                CGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTA                                                                                                                      >  SRR19973078.3577536/1‑150 (MQ=60)
                                  AACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATTTTTTTCTCCTTTCGCTATACATCCTAAG                                                                                                                    >  SRR19973078.2708459/1‑150 (MQ=60)
                                  AACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATTTTTTTCTCCTTTCGCTATACATCCTAAG                                                                                                                    >  SRR19973078.2708458/1‑150 (MQ=60)
                                  AACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAG                                                                                                                    >  SRR19973078.895464/1‑150 (MQ=60)
                                  AACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAG                                                                                                                    >  SRR19973078.3337261/1‑150 (MQ=60)
                                  AACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAG                                                                                                                    >  SRR19973078.3336714/1‑150 (MQ=60)
                                     ATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAG                                                                                                                 <  SRR19973078.2416619/150‑1 (MQ=60)
                                        ATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTAT                                                                                                              <  SRR19973078.4722878/150‑1 (MQ=60)
                                            ATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCG                                                                                                          <  SRR19973078.57724/150‑1 (MQ=60)
                                            ATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCG                                                                                                          <  SRR19973078.288973/150‑1 (MQ=60)
                                                  TCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATTTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGA                                                                                                    <  SRR19973078.179081/150‑1 (MQ=60)
                                                  TCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGA                                                                                                    <  SRR19973078.3529289/150‑1 (MQ=60)
                                                  TCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGA                                                                                                    <  SRR19973078.3468817/150‑1 (MQ=60)
                                                  TCCTGAAGGTGCATGTTGTTATCGATGTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGA                                                                                                    <  SRR19973078.1289678/150‑1 (MQ=60)
                                                     TGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAAT                                                                                                 <  SRR19973078.573897/150‑1 (MQ=60)
                                                     TGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGAGGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAAT                                                                                                 >  SRR19973078.4447898/1‑150 (MQ=60)
                                                        AGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATTTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTT                                                                                              <  SRR19973078.198874/150‑1 (MQ=60)
                                                          GTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGA                                                                                            <  SRR19973078.1832782/150‑1 (MQ=60)
                                                                GTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATT                                                                                      <  SRR19973078.2835551/150‑1 (MQ=60)
                                                                        CGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTCTTTCGGCGTGAAAGTTTGATTTATTTCACATAG                                                                              >  SRR19973078.3047149/1‑150 (MQ=60)
                                                                          ATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATTTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAG                                                                            >  SRR19973078.1349971/1‑150 (MQ=60)
                                                                          ATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATTTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAG                                                                            >  SRR19973078.1350768/1‑150 (MQ=60)
                                                                          ATGTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAG                                                                            <  SRR19973078.3260822/150‑1 (MQ=60)
                                                                             TGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTA                                                                         <  SRR19973078.2589900/150‑1 (MQ=60)
                                                                             TGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTA                                                                         <  SRR19973078.2120954/150‑1 (MQ=60)
                                                                             TGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTA                                                                         <  SRR19973078.182345/150‑1 (MQ=60)
                                                                                   GAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTT                                                                   <  SRR19973078.1416109/150‑1 (MQ=60)
                                                                                    AACGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTATTGGTTT                                                                  >  SRR19973078.3521707/1‑150 (MQ=60)
                                                                                          GTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATT                                                            <  SRR19973078.4514131/150‑1 (MQ=60)
                                                                                          GTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATT                                                            <  SRR19973078.1957121/150‑1 (MQ=60)
                                                                                              AATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATT                                                        >  SRR19973078.1401490/1‑150 (MQ=60)
                                                                                              AATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATT                                                        <  SRR19973078.4496780/150‑1 (MQ=60)
                                                                                              AATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATT                                                        >  SRR19973078.4497700/1‑150 (MQ=60)
                                                                                               ATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTT                                                       <  SRR19973078.2368560/150‑1 (MQ=60)
                                                                                                        ACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTT                                              <  SRR19973078.3008366/150‑1 (MQ=60)
                                                                                                        ACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTT                                              <  SRR19973078.3008699/150‑1 (MQ=60)
                                                                                                          TGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATGTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTATTGGTTTTCTATTTATTTAATGATTTTAA                                            >  SRR19973078.968652/1‑150 (MQ=60)
                                                                                                           GATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAG                                           >  SRR19973078.2523841/1‑150 (MQ=60)
                                                                                                           GATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAG                                           >  SRR19973078.2523805/1‑150 (MQ=60)
                                                                                                            ATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGT                                          >  SRR19973078.3531924/1‑150 (MQ=60)
                                                                                                            ATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGT                                          >  SRR19973078.316201/1‑150 (MQ=60)
                                                                                                             TAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATTTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATATAGTTAGTGGTTTTCTATTTATTTAATGTTTTTATGTT                                         >  SRR19973078.2841172/1‑150 (MQ=60)
                                                                                                             TAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATTTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTT                                         >  SRR19973078.434166/1‑150 (MQ=60)
                                                                                                             TAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATTTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTT                                         >  SRR19973078.434182/1‑150 (MQ=60)
                                                                                                               ACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTT                                       <  SRR19973078.4036606/150‑1 (MQ=60)
                                                                                                                CCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTT                                      <  SRR19973078.2165228/150‑1 (MQ=60)
                                                                                                                CCTTTTATTGTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTT                                      <  SRR19973078.4578693/150‑1 (MQ=60)
                                                                                                                   TTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAA                                   <  SRR19973078.2473791/150‑1 (MQ=60)
                                                                                                                    TTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAAT                                  >  SRR19973078.2438653/1‑150 (MQ=60)
                                                                                                                    TTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAAT                                  >  SRR19973078.2437328/1‑150 (MQ=60)
                                                                                                                    TTATTTTCACTTGGGAGAAAGGGGGTGATCGAGGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTTTATTTATTTAATGATTTTAAGTTTTTTAAT                                  >  SRR19973078.945951/1‑150 (MQ=60)
                                                                                                                    TTATTTTCACTTGGGAGAAAGGGGGTGATCGAGGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTTTATTTATTTAATGATTTTAAGTTTTTTAAT                                  >  SRR19973078.944634/1‑150 (MQ=60)
                                                                                                                        TTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAAT                              <  SRR19973078.296289/150‑1 (MQ=60)
                                                                                                                         TTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATG                             <  SRR19973078.1987642/150‑1 (MQ=60)
                                                                                                                         TTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATG                             <  SRR19973078.4389836/150‑1 (MQ=60)
                                                                                                                         TTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATG                             <  SRR19973078.4388998/150‑1 (MQ=60)
                                                                                                                         TTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATG                             <  SRR19973078.4226170/150‑1 (MQ=60)
                                                                                                                          TCACTTGGGAGAAAGGGGGTGATCGATGTATATTTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATTT                            >  SRR19973078.4154822/1‑150 (MQ=60)
                                                                                                                          TCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGT                            <  SRR19973078.4469404/150‑1 (MQ=60)
                                                                                                                          TCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGT                            <  SRR19973078.4469366/150‑1 (MQ=60)
                                                                                                                           CACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTA                           >  SRR19973078.2903227/1‑150 (MQ=60)
                                                                                                                           CACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTA                           <  SRR19973078.3069342/150‑1 (MQ=60)
                                                                                                                           CACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTA                           <  SRR19973078.727521/150‑1 (MQ=60)
                                                                                                                             CTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAAT                         >  SRR19973078.4797616/1‑150 (MQ=60)
                                                                                                                             CTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAAT                         >  SRR19973078.1415288/1‑150 (MQ=60)
                                                                                                                             CTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAAT                         >  SRR19973078.3796847/1‑150 (MQ=60)
                                                                                                                                 GGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACG                     <  SRR19973078.3937577/150‑1 (MQ=60)
                                                                                                                                         GGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAATGACT             <  SRR19973078.1121252/150‑1 (MQ=60)
                                                                                                                                             TGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAgatcggaagagcacacgt         >  SRR19973078.3848559/1‑132 (MQ=60)
                                                                                                                                             TGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAATGACTCGCA         <  SRR19973078.3426360/150‑1 (MQ=60)
                                                                                                                                             TGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAATGACTCGCA         <  SRR19973078.3428013/150‑1 (MQ=60)
                                                                                                                                                    TGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAATGACTCGCAGGTTTAA  <  SRR19973078.1675783/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
TGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGAGGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTTTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAATGACTCGCAGGTTTAA  >  CP009273/647274‑647571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑