Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F218 I0 R1	1506	278.7	9275112	94.5%	8764980	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	647,422	G→T	100%	intergenic (‑110/‑269)	citC ← / → citA	[citrate [pro‑3S]‑lyase] ligase/sensory histidine kinase in two‑component regulatory system with CitB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	647,422	0	G	T	98.3%	783.3 / 4.1	232	intergenic (‑110/‑269)	citC/citA	[citrate [pro‑3S]‑lyase] ligase/sensory histidine kinase in two‑component regulatory system with CitB
Reads supporting (aligned to +/- strand): ref base G (2/2); new base T (120/108); total (122/110)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 232 total aligned reads displayed.

GAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGAGGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTTTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAATGACTCGCAGGTT  >  CP009273/647275‑647568

gAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGt                                                                                                                                                  <  2:1582662/150‑1 (MQ=255)

   cGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGtata                                                                                                                                               >  2:1650169/1‑150 (MQ=255)

   cGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGtata                                                                                                                                               >  2:2815955/1‑150 (MQ=255)

     ttttACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATc                                                                                                                                             >  1:3015436/1‑150 (MQ=255)

     ttttACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATc                                                                                                                                             >  1:4005392/1‑150 (MQ=255)

       ttACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCtt                                                                                                                                           >  2:670212/1‑150 (MQ=255)

       ttACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCtt                                                                                                                                           >  2:2590414/1‑150 (MQ=255)

       ttACGCGGGTGAAAATATCATTGCCAAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCtt                                                                                                                                           <  1:3657223/150‑1 (MQ=255)

         aCGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCtttt                                                                                                                                         <  1:3333847/150‑1 (MQ=255)

         aCGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGAGGTATATCtttt                                                                                                                                         >  2:3882758/1‑150 (MQ=255)

            cgGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTtct                                                                                                                                      <  2:3718539/150‑1 (MQ=255)

            cgGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTtct                                                                                                                                      <  2:3023702/150‑1 (MQ=255)

             gggTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTtctc                                                                                                                                     <  1:132501/150‑1 (MQ=255)

              ggTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTcc                                                                                                                                    >  1:537932/1‑150 (MQ=255)

                  aaaaTATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTc                                                                                                                                >  2:4409687/1‑150 (MQ=255)

                       atCATTGCCGAACATAATAAATAGTATCCTCAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCtat                                                                                                                           >  1:4287229/1‑150 (MQ=255)

                        tCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCtata                                                                                                                          <  1:1013275/150‑1 (MQ=255)

                         cATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATAc                                                                                                                         <  1:4527540/150‑1 (MQ=255)

                         cATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATAc                                                                                                                         >  2:2235906/1‑150 (MQ=255)

                         cATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATAc                                                                                                                         >  2:4260047/1‑150 (MQ=255)

                             gCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATcc                                                                                                                     >  1:625032/1‑150 (MQ=255)

                             gCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATcc                                                                                                                     >  1:625116/1‑150 (MQ=255)

                             gCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATcc                                                                                                                     >  2:4162899/1‑150 (MQ=255)

                              ccGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCt                                                                                                                    >  1:2334790/1‑150 (MQ=255)

                                gAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTaa                                                                                                                  >  2:906118/1‑150 (MQ=255)

                                gAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTaa                                                                                                                  >  1:1890970/1‑150 (MQ=255)

                                  aCATAATAAATAGTATCCTGAAGGTGTATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAgg                                                                                                                <  2:4036704/150‑1 (MQ=255)

                                   cATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGa                                                                                                               <  2:3091790/150‑1 (MQ=255)

                                      aataaataGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATNGAGGTATATNTTTTTCTCCTTTCGCTATACATCCTAAGGAGTa                                                                                                            >  1:1903265/1‑150 (MQ=255)

                                         aaataGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTAttt                                                                                                         >  1:1672568/1‑150 (MQ=255)

                                             aGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGc                                                                                                     >  1:2525742/1‑150 (MQ=255)

                                                 tCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGa                                                                                                 <  1:264567/150‑1 (MQ=255)

                                                 tCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGa                                                                                                 >  1:584235/1‑150 (MQ=255)

                                                      aaGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAAttt                                                                                            >  2:3305075/1‑150 (MQ=255)

                                                       aGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAAtttt                                                                                           <  1:3758116/150‑1 (MQ=255)

                                                       aGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAAtttt                                                                                           >  2:11965/1‑150 (MQ=255)

                                                       aGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAAtttt                                                                                           <  1:1393936/150‑1 (MQ=255)

                                                          tGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGat                                                                                        >  2:2185920/1‑150 (MQ=255)

                                                          tGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGat                                                                                        >  1:2598704/1‑150 (MQ=255)

                                                             aTGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAATTTGTGattta                                                                                     >  2:4351474/1‑150 (MQ=255)

                                                             aTGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGattta                                                                                     >  2:4351537/1‑150 (MQ=255)

                                                                   ttATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTcac                                                                               <  1:1097267/150‑1 (MQ=255)

                                                                   ttATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTcac                                                                               <  2:4553952/150‑1 (MQ=255)

                                                                    tATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTcaca                                                                              <  1:1820748/150‑1 (MQ=255)

                                                                     aTCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACAt                                                                             <  2:3506587/150‑1 (MQ=255)

                                                                      tCGATGTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATa                                                                            <  2:3212410/150‑1 (MQ=255)

                                                                       cGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATag                                                                           >  1:3830290/1‑150 (MQ=255)

                                                                        gATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATaga                                                                          >  1:1677575/1‑150 (MQ=255)

                                                                        gATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATaga                                                                          >  1:794538/1‑150 (MQ=255)

                                                                           ttGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGtt                                                                       >  1:3253496/1‑150 (MQ=255)

                                                                             gCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAg                                                                     <  1:3873989/150‑1 (MQ=255)

                                                                                  gAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGtt                                                                <  1:4460307/150‑1 (MQ=255)

                                                                                  gAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGtt                                                                <  2:3713259/150‑1 (MQ=255)

                                                                                   aaTGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGttt                                                               <  2:4603322/150‑1 (MQ=255)

                                                                                    aTGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGtttt                                                              <  2:2591096/150‑1 (MQ=255)

                                                                                    aTGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGtttt                                                              >  1:3646765/1‑150 (MQ=255)

                                                                                     tgttgtTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGttttc                                                             <  1:342268/150‑2 (MQ=255)

                                                                                      gttgttCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGttttct                                                            >  1:980099/1‑148 (MQ=255)

                                                                                      gttgttCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGttttct                                                            >  1:4134306/1‑148 (MQ=255)

                                                                                         gttCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTtctatt                                                         >  2:4003139/1‑150 (MQ=255)

                                                                                           tCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTtctattta                                                       >  1:2401648/1‑150 (MQ=255)

                                                                                           tCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTtctattta                                                       <  2:3005304/150‑1 (MQ=255)

                                                                                            cAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTtctatttat                                                      <  2:2502907/150‑1 (MQ=255)

                                                                                             aaTGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTtctatttatt                                                     >  1:1254287/1‑150 (MQ=255)

                                                                                               tGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTtctatttattta                                                   <  2:1297796/150‑1 (MQ=255)

                                                                                               tGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTtctatttattta                                                   >  1:428625/1‑150 (MQ=255)

                                                                                               tGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTtctatttattta                                                   <  1:1005832/150‑1 (MQ=255)

                                                                                                  tGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATg                                                <  2:1860350/150‑1 (MQ=255)

                                                                                                  tGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATg                                                >  1:2726907/1‑150 (MQ=255)

                                                                                                   gCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGa                                               <  1:305463/150‑1 (MQ=255)

                                                                                                     aaaCTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGAtt                                             <  2:1373325/150‑1 (MQ=255)

                                                                                                     aaaCTGATAACCTTTTATTTTCACTGGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGAtt                                             <  2:2161560/150‑1 (MQ=255)

                                                                                                      aaCTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGAttt                                            >  2:2050862/1‑150 (MQ=255)

                                                                                                       aCTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGAtttt                                           >  2:2391573/1‑150 (MQ=255)

                                                                                                       aCTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGAtttt                                           <  2:79855/150‑1 (MQ=255)

                                                                                                        cTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTa                                          >  1:3967087/1‑150 (MQ=255)

                                                                                                         tGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTaa                                         >  2:4115872/1‑150 (MQ=255)

                                                                                                           aTAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGt                                       >  2:294870/1‑150 (MQ=255)

                                                                                                             aaCCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGttt                                     >  2:2656471/1‑150 (MQ=255)

                                                                                                                cTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTGTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGtttttt                                  <  2:3344422/150‑1 (MQ=255)

                                                                                                                  tttATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTATGTTTTttaa                                >  2:4531920/1‑150 (MQ=255)

                                                                                                                     aTTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTttaatta                             <  1:67381/150‑1 (MQ=255)

                                                                                                                     aTTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTttaatta                             <  1:3843371/150‑1 (MQ=255)

                                                                                                                      ttttCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTttaattaa                            <  1:928946/150‑1 (MQ=255)

                                                                                                                       tttCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTtaattaat                           >  1:1064866/1‑150 (MQ=255)

                                                                                                                        ttCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATg                          <  1:4322500/150‑1 (MQ=255)

                                                                                                                         tCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGt                         <  1:1387311/150‑1 (MQ=255)

                                                                                                                          cACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTa                        >  2:4011304/1‑150 (MQ=255)

                                                                                                                          cACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTa                        >  1:2701115/1‑150 (MQ=255)

                                                                                                                              tGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTa                    <  1:1652457/150‑1 (MQ=255)

                                                                                                                              tGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTa                    <  1:2390477/150‑1 (MQ=255)

                                                                                                                              tGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTa                    <  2:171325/150‑1 (MQ=255)

                                                                                                                                 gagaAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGa                 >  2:2450514/1‑150 (MQ=255)

                                                                                                                                  agaAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGaa                >  1:2217545/1‑150 (MQ=255)

                                                                                                                                   gaAAGGGGGTGATCGATGTATATCATTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGaaa               <  1:1697331/150‑1 (MQ=255)

                                                                                                                                    aaaGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAAt              <  1:3200721/150‑1 (MQ=255)

                                                                                                                                      aGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAATGa            >  2:1662972/1‑150 (MQ=255)

                                                                                                                                            tGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAATGACTCgaa      >  2:1309683/1‑148 (MQ=255)

                                                                                                                                              aTCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAATGACTCGCAgg    <  1:3505259/150‑1 (MQ=255)

                                                                                                                                                cGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAATGACTCGCAGGtt  >  1:3121636/1‑150 (MQ=255)

GAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGAGGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTTTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAATGACTCGCAGGTT  >  CP009273/647275‑647568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGAGGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTTTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAATGACTCGCAGGTTT  >  CP009273/647274‑647569

TGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATG                                                                                                                                                    <  SRR19973073.1375048/150‑1 (MQ=60)

TGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATG                                                                                                                                                    >  SRR19973073.1210009/1‑150 (MQ=60)

      TTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATC                                                                                                                                              >  SRR19973073.3021545/1‑150 (MQ=60)

      TTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATC                                                                                                                                              >  SRR19973073.4013387/1‑150 (MQ=60)

        TTACGCGGGTGAAAATATCATTGCCAAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTT                                                                                                                                            <  SRR19973073.3664559/150‑1 (MQ=60)

          ACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTT                                                                                                                                          <  SRR19973073.3340478/150‑1 (MQ=60)

          ACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTT                                                                                                                                          <  SRR19973073.3340590/150‑1 (MQ=60)

           CGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTT                                                                                                                                         >  SRR19973073.33774/1‑150 (MQ=60)

              GGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTC                                                                                                                                      <  SRR19973073.132823/150‑1 (MQ=60)

              GGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTC                                                                                                                                      >  SRR19973073.4198390/1‑150 (MQ=60)

               GGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCC                                                                                                                                     >  SRR19973073.539093/1‑150 (MQ=60)

                 TGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTT                                                                                                                                   <  SRR19973073.1465433/150‑1 (MQ=60)

                     AATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGC                                                                                                                               >  SRR19973073.3200596/1‑150 (MQ=60)

                        ATCATTGCCGAACATAATAAATAGTATCCTCAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTAT                                                                                                                            >  SRR19973073.4295759/1‑150 (MQ=60)

                         TCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATA                                                                                                                           <  SRR19973073.1015396/150‑1 (MQ=60)

                          CATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATAC                                                                                                                          <  SRR19973073.4536635/150‑1 (MQ=60)

                          CATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATAC                                                                                                                          <  SRR19973073.4536622/150‑1 (MQ=60)

                              GCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCC                                                                                                                      >  SRR19973073.626449/1‑150 (MQ=60)

                              GCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCC                                                                                                                      >  SRR19973073.626365/1‑150 (MQ=60)

                              GCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCC                                                                                                                      >  SRR19973073.626372/1‑150 (MQ=60)

                               CCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCT                                                                                                                     >  SRR19973073.2339551/1‑150 (MQ=60)

                                CGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTA                                                                                                                    >  SRR19973073.1317777/1‑150 (MQ=60)

                                 GAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAA                                                                                                                   >  SRR19973073.1894831/1‑150 (MQ=60)

                                  AACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAG                                                                                                                  >  SRR19973073.1085604/1‑150 (MQ=60)

                                   ACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGG                                                                                                                 <  SRR19973073.2565005/150‑1 (MQ=60)

                                      TAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGT                                                                                                              <  SRR19973073.1010440/150‑1 (MQ=60)

                                       AATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATNGAGGTATATNTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA                                                                                                             >  SRR19973073.1907140/1‑150 (MQ=60)

                                       AATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA                                                                                                             >  SRR19973073.2640056/1‑150 (MQ=60)

                                       AATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTA                                                                                                             >  SRR19973073.3649063/1‑150 (MQ=60)

                                         TAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATT                                                                                                           >  SRR19973073.4383589/1‑150 (MQ=60)

                                         TAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATT                                                                                                           >  SRR19973073.1848628/1‑150 (MQ=60)

                                          AAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTT                                                                                                          >  SRR19973073.1676039/1‑150 (MQ=60)

                                          AAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTT                                                                                                          >  SRR19973073.4004855/1‑150 (MQ=60)

                                              AGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGC                                                                                                      >  SRR19973073.2530959/1‑150 (MQ=60)

                                                  TCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGA                                                                                                  <  SRR19973073.265149/150‑1 (MQ=60)

                                                  TCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGA                                                                                                  >  SRR19973073.585486/1‑150 (MQ=60)

                                                   CCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAA                                                                                                 >  SRR19973073.1679550/1‑150 (MQ=60)

                                                      GAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATT                                                                                              <  SRR19973073.1224024/150‑1 (MQ=60)

                                                      GAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGAGGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATT                                                                                              <  SRR19973073.3306587/150‑1 (MQ=60)

                                                       AAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTT                                                                                             >  SRR19973073.4300237/1‑150 (MQ=60)

                                                        AGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTT                                                                                            <  SRR19973073.1396885/150‑1 (MQ=60)

                                                        AGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTT                                                                                            <  SRR19973073.1396882/150‑1 (MQ=60)

                                                        AGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTT                                                                                            <  SRR19973073.3765648/150‑1 (MQ=60)

                                                           TGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGAT                                                                                         >  SRR19973073.2604051/1‑150 (MQ=60)

                                                            GCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATT                                                                                        <  SRR19973073.382338/150‑1 (MQ=60)

                                                             CATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTT                                                                                       >  SRR19973073.3049251/1‑150 (MQ=60)

                                                                    TTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCAC                                                                                <  SRR19973073.2730693/150‑1 (MQ=60)

                                                                    TTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCAC                                                                                <  SRR19973073.1099521/150‑1 (MQ=60)

                                                                     TATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACA                                                                               <  SRR19973073.1824512/150‑1 (MQ=60)

                                                                     TATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACA                                                                               >  SRR19973073.3919035/1‑150 (MQ=60)

                                                                     TATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACA                                                                               >  SRR19973073.3918908/1‑150 (MQ=60)

                                                                        CGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAG                                                                            >  SRR19973073.3837907/1‑150 (MQ=60)

                                                                         GATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGA                                                                           >  SRR19973073.1681062/1‑150 (MQ=60)

                                                                         GATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGA                                                                           >  SRR19973073.796146/1‑150 (MQ=60)

                                                                         GATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGA                                                                           >  SRR19973073.795885/1‑150 (MQ=60)

                                                                         GATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGA                                                                           >  SRR19973073.1681054/1‑150 (MQ=60)

                                                                          ATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAG                                                                          <  SRR19973073.1699500/150‑1 (MQ=60)

                                                                          ATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAG                                                                          <  SRR19973073.1065930/150‑1 (MQ=60)

                                                                            TTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTT                                                                        >  SRR19973073.3260075/1‑150 (MQ=60)

                                                                              GCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAG                                                                      <  SRR19973073.3881667/150‑1 (MQ=60)

                                                                              GCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAG                                                                      <  SRR19973073.2525527/150‑1 (MQ=60)

                                                                                AACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTG                                                                    <  SRR19973073.1666664/150‑1 (MQ=60)

                                                                                 ACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGG                                                                   <  SRR19973073.3802572/150‑1 (MQ=60)

                                                                                   GAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTT                                                                 <  SRR19973073.275653/150‑1 (MQ=60)

                                                                                   GAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTT                                                                 <  SRR19973073.4469271/150‑1 (MQ=60)

                                                                                     ATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTT                                                               >  SRR19973073.3080690/1‑150 (MQ=60)

                                                                                     ATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTT                                                               >  SRR19973073.3654081/1‑150 (MQ=60)

                                                                                     ATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTT                                                               <  SRR19973073.3477386/150‑1 (MQ=60)

                                                                                      TGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTC                                                              >  SRR19973073.565584/1‑150 (MQ=60)

                                                                                      TGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTC                                                              <  SRR19973073.342960/150‑1 (MQ=60)

                                                                                       GTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCT                                                             >  SRR19973073.4142575/1‑150 (MQ=60)

                                                                                       GTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCT                                                             >  SRR19973073.982142/1‑150 (MQ=60)

                                                                                         TGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTAT                                                           <  SRR19973073.1101430/150‑1 (MQ=60)

                                                                                           TTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTT                                                         <  SRR19973073.1889127/150‑1 (MQ=60)

                                                                                            TCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTA                                                        >  SRR19973073.2406577/1‑150 (MQ=60)

                                                                                            TCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTA                                                        >  SRR19973073.2406587/1‑150 (MQ=60)

                                                                                             CAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTAT                                                       >  SRR19973073.2666821/1‑150 (MQ=60)

                                                                                              AATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATT                                                      <  SRR19973073.2463303/150‑1 (MQ=60)

                                                                                              AATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATT                                                      >  SRR19973073.1256909/1‑150 (MQ=60)

                                                                                                TGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTCTTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTA                                                    >  SRR19973073.429480/1‑150 (MQ=60)

                                                                                                TGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTA                                                    >  SRR19973073.429504/1‑150 (MQ=60)

                                                                                                TGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTA                                                    >  SRR19973073.429483/1‑150 (MQ=60)

                                                                                                TGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTA                                                    >  SRR19973073.4404095/1‑150 (MQ=60)

                                                                                                TGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTA                                                    <  SRR19973073.1007935/150‑1 (MQ=60)

                                                                                                TGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTA                                                    <  SRR19973073.3659460/150‑1 (MQ=60)

                                                                                                   TGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATG                                                 >  SRR19973073.2732473/1‑150 (MQ=60)

                                                                                                    GCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGA                                                <  SRR19973073.306103/150‑1 (MQ=60)

                                                                                                    GCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGA                                                >  SRR19973073.3859281/1‑150 (MQ=60)

                                                                                                      AAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATT                                              >  SRR19973073.3725239/1‑150 (MQ=60)

                                                                                                      AAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATT                                              >  SRR19973073.634205/1‑150 (MQ=60)

                                                                                                       AACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTT                                             <  SRR19973073.4530500/150‑1 (MQ=60)

                                                                                                         CTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTA                                           >  SRR19973073.3974989/1‑150 (MQ=60)

                                                                                                             TAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTT                                       >  SRR19973073.1902373/1‑150 (MQ=60)

                                                                                                             TAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTT                                       <  SRR19973073.3527022/150‑1 (MQ=60)

                                                                                                                 CTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTT                                   <  SRR19973073.2676205/150‑1 (MQ=60)

                                                                                                                 CTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTT                                   <  SRR19973073.2316502/150‑1 (MQ=60)

                                                                                                                   TTTATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAA                                 <  SRR19973073.2269211/150‑1 (MQ=60)

                                                                                                                      ATTTTCACTTTGGAGAAAGGGTGTGATCGATGTATATCATTTTCTCCTTTCGATATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCAAATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTA                              <  SRR19973073.3850981/150‑1 (MQ=60)

                                                                                                                      ATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTA                              >  SRR19973073.1976420/1‑150 (MQ=60)

                                                                                                                      ATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTA                              <  SRR19973073.67572/150‑1 (MQ=60)

                                                                                                                      ATTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTA                              <  SRR19973073.3850996/150‑1 (MQ=60)

                                                                                                                       TTTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAA                             <  SRR19973073.930882/150‑1 (MQ=60)

                                                                                                                        TTTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAAT                            >  SRR19973073.1067069/1‑150 (MQ=60)

                                                                                                                         TTCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATG                           <  SRR19973073.4331136/150‑1 (MQ=60)

                                                                                                                          TCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGT                          <  SRR19973073.1390246/150‑1 (MQ=60)

                                                                                                                          TCACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGT                          <  SRR19973073.1390633/150‑1 (MQ=60)

                                                                                                                           CACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTA                         >  SRR19973073.2706629/1‑150 (MQ=60)

                                                                                                                           CACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTA                         <  SRR19973073.808880/150‑1 (MQ=60)

                                                                                                                            ACTTGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAA                        >  SRR19973073.3242214/1‑150 (MQ=60)

                                                                                                                               TGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTA                     <  SRR19973073.1655880/150‑1 (MQ=60)

                                                                                                                               TGGGAGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTA                     <  SRR19973073.2395384/150‑1 (MQ=60)

                                                                                                                                   AGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAA                 >  SRR19973073.636705/1‑150 (MQ=60)

                                                                                                                                   AGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAA                 >  SRR19973073.2222141/1‑150 (MQ=60)

                                                                                                                                   AGAAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAA                 >  SRR19973073.4108106/1‑150 (MQ=60)

                                                                                                                                    GAAAGGGGGTGATCGATGTATATCATTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAA                <  SRR19973073.1700852/150‑1 (MQ=60)

                                                                                                                                     AAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAAT               <  SRR19973073.3207191/150‑1 (MQ=60)

                                                                                                                                     AAAGGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAAT               <  SRR19973073.949228/150‑1 (MQ=60)

                                                                                                                                        GGGGGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAATGAC            <  SRR19973073.1738938/150‑1 (MQ=60)

                                                                                                                                           GGTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAATGACTCG         >  SRR19973073.539916/1‑150 (MQ=60)

                                                                                                                                            GTGATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAATGACTCGC        >  SRR19973073.1357532/1‑150 (MQ=60)

                                                                                                                                               ATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAATGACTCGCAGG     >  SRR19973073.620953/1‑150 (MQ=60)

                                                                                                                                               ATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAATGACTCGCAGG     >  SRR19973073.234943/1‑150 (MQ=60)

                                                                                                                                               ATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAATGACTCGCAGG     <  SRR19973073.3041715/150‑1 (MQ=60)

                                                                                                                                               ATCGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAATGACTCGCAGG     <  SRR19973073.3512260/150‑1 (MQ=60)

                                                                                                                                                 CGATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAATGACTCGCAGGTT   >  SRR19973073.3127914/1‑150 (MQ=60)

                                                                                                                                                  GATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAATGACTCGCAGGTTT  <  SRR19973073.4204393/150‑1 (MQ=60)

                                                                                                                                                  GATGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTCTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAATGACTCGCAGGTTT  <  SRR19973073.4204384/150‑1 (MQ=60)

TGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAACTGATAACCTTTTATTTTCACTTGGGAGAAAGGGGGTGATCGAGGTATATCTTTTTCTCCTTTCGCTATACATCCTAAGGAGTATTTCGGCGTGAAATTTTGATTTATTTCACATAGAGTTAGTGGTTTTTTATTTATTTAATGATTTTAAGTTTTTTAATTAATGTAATTACGAAATGACTCGCAGGTTT  >  CP009273/647274‑647569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`