Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F309 I0 R1	1697	197.2	6627756	95.7%	6342762	147.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,665,772	G→T	100%	E123* (GAA→TAA)	hcaD →	phenylpropionate dioxygenase, ferredoxin reductase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,665,772	0	G	T	95.3%	353.3 / 8.2	130	E123* (GAA→TAA)	hcaD	phenylpropionate dioxygenase, ferredoxin reductase subunit
Reads supporting (aligned to +/- strand): ref base G (3/4); new base T (55/67); total (59/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 130 total aligned reads displayed.

TCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGA  >  CP009273/2665627‑2665916

tCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATaa                                                                                                                                                 <  2:2462073/148‑1 (MQ=255)

 cTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAAc                                                                                                                                                >  2:2714552/1‑148 (MQ=255)

    cATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCCCGGCCGTTGCGTGTTGCACTGGGATAAccca                                                                                                                                             >  1:3279500/1‑145 (MQ=255)

     aTTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACgctg                                                                                                                                            <  2:3263431/148‑1 (MQ=255)

      ttCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGGAGCTGGCCCCCTGCCGTTGCGTGATGCACTGGGATAACgctgc                                                                                                                                           >  1:207482/1‑148 (MQ=255)

       tCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACgctgc‑t                                                                                                                                         <  2:2710073/148‑1 (MQ=255)

            tgtAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGC‑TTTAcc                                                                                                                                    >  2:654717/1‑148 (MQ=255)

             gtAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGC‑TTTAccc                                                                                                                                   >  1:2994441/1‑148 (MQ=255)

               aaCCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGC‑TTTACCCTa                                                                                                                                 <  2:2729034/148‑1 (MQ=255)

                 ccATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGC‑TTTACCCTACg                                                                                                                               <  1:1813848/148‑1 (MQ=255)

                   aTCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCGCGACCCCAGCCGGTGCTTGATGCACTGGGCTAACGCTGC‑TTTAACCTACGcc                                                                                                                             >  1:1843254/1‑148 (MQ=255)

                     cAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCGCGACCCCGGCCCGTGCGTGATGCACTGGGGGAACGCTGC‑TGTTACCGACGccca                                                                                                                           >  1:2296058/1‑146 (MQ=255)

                       aaaCGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGc                                                                                                                         <  1:509153/148‑1 (MQ=255)

                           gCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGt                                                                                                                     <  2:743317/148‑1 (MQ=255)

                               ggTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATg                                                                                                                 <  2:2544957/148‑1 (MQ=255)

                               ggTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCGTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGC‑TTGACCCTACGCCATGCCGGTGATg                                                                                                                 <  2:524406/148‑1 (MQ=255)

                               ggTCGCGACACACGAGAGTGAGTGTTAACCACCGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATg                                                                                                                 <  2:2187071/148‑1 (MQ=255)

                                   gcgACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATgccgc                                                                                                             <  2:304330/148‑1 (MQ=255)

                                   gcgACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGC‑TTTACCCTACGCCATGCCGGTGATgccgc                                                                                                             <  1:1138368/148‑1 (MQ=255)

                                     gACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCa                                                                                                           <  2:1879430/148‑1 (MQ=255)

                                       cacacGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGa                                                                                                         <  1:2667508/148‑1 (MQ=255)

                                       cacacGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGa                                                                                                         <  1:2663854/148‑1 (MQ=255)

                                        acacGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGAc                                                                                                        <  1:82330/148‑1 (MQ=255)

                                        acacGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGAc                                                                                                        <  1:1451997/148‑1 (MQ=255)

                                           cGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGGCGCGCGACCCCGGCCCGTGGTTGTTTGACAGGGGTTAAGCGGC‑GTTAACCAACGCCAAGCCGGTGATGCCGCCAgcaagc                                                                                                     >  1:317652/1‑143 (MQ=255)

                                            gagaGTTAGTGGTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCg                                                                                                    <  2:2809781/148‑1 (MQ=255)

                                             agagTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCga                                                                                                   <  2:2411037/148‑1 (MQ=255)

                                              gagTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCCGCGCGCCCGCTGCCGTTGGTTGATTCCCTGGGTTAACGCGGG‑TTTTACCTAAGCCCAGGCGGGGATGGCCCCCGACTgggcg                                                                                                  >  1:1308320/1‑144 (MQ=255)

                                                 ttAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAg                                                                                               <  1:2945308/148‑1 (MQ=255)

                                                 ttAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAg                                                                                               <  1:2945262/148‑1 (MQ=255)

                                                 ttAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAg                                                                                               <  1:2716629/148‑1 (MQ=255)

                                                  tAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGt                                                                                              <  1:561333/148‑1 (MQ=255)

                                                  tAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCCCTGCCGGTGCTTTATTCCCTGGGGTAACGCGGC‑GTTAACCAAAGCCAAACCCGTGATTCCGCCAGAAAGCGAGAAGt                                                                                              >  1:1395048/1‑148 (MQ=255)

                                                   aGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGtt                                                                                             <  1:2527110/148‑1 (MQ=255)

                                                      gtTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGGCGCGCGACCGCGGCCGGTGGGTGTTTCACGGGGATCAAGCTGC‑GTTTCCCCACGCCCAGCCCGGGGTGCCGCCCGACAGCGCGAACGTCTg                                                                                          >  1:2935673/1‑148 (MQ=255)

                                                          aCCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCCGCTCGACCGCTGCCGTTGCGTGATGCCCTGGGATAACGCTGC‑TTTACCCAACGCCAGGCCGGTGATGCCGCCAGACAGCGAGAAGTTCTGCAGc                                                                                      >  2:817311/1‑148 (MQ=255)

                                                          aCCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCCGCTCGACCGCTGCCGTTGCGTGATGCACTGGGATAACGCTGC‑TTTACCCGACCCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGc                                                                                      >  2:1865742/1‑148 (MQ=255)

                                                            cAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCGCGACCGCTGCCGTTGCTTGTTGCACTGGGATAACCCTGC‑TTTACCCTACGCCAAGCCGGGGATGCCGCCCGACTGCGAGAAGTTGTGCAGccc                                                                                    >  2:3222927/1‑148 (MQ=255)

                                                              ccGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGGTGCTTGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGa                                                                                  <  1:121367/147‑1 (MQ=255)

                                                              aCGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGa                                                                                  <  1:1794910/148‑1 (MQ=255)

                                                                    aaaGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGTCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTc                                                                            <  1:1646113/148‑1 (MQ=255)

                                                                    aaaGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCCTTGCATGAAGCACAGGGATAAACCTGC‑TGTTCCCAAAGCCAAGCCGGTGATTCCGCCAGACAGCGACAAGTTCTGCAGCCCGAAccctc                                                                            >  2:1937826/1‑144 (MQ=255)

                                                                     aaGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCa                                                                           <  2:2748377/148‑1 (MQ=255)

                                                                      aGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCCGTGCATGATGCACGGGGCTAACGCGGC‑TTTACCCAACGCCAAGCCGGTGATGCCCCCCGACAGCCAGAAGTTCAGCAGCCCCAACCGTccg                                                                          >  2:1544511/1‑146 (MQ=255)

                                                                       gCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGt                                                                         <  1:102886/148‑1 (MQ=255)

                                                                           gCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATTCACTGGGATAACGCTGC‑GTTTACCAACCCCAAGCCGGTGATTCCGCCAGACAGCCAGAAGTTATGCAGCCCCAAACGTCAGGCGTg                                                                     >  1:2178119/1‑148 (MQ=255)

                                                                           gCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGCTAACGCTGC‑GTTACCCTACGCCCAGCCGGTGATTCCGCCAGACAGCGAGAAGTTCGGCAGCCCCAACCGTCAGTCGTg                                                                     >  1:2178226/1‑148 (MQ=255)

                                                                            cACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGa                                                                    <  2:2433163/148‑1 (MQ=255)

                                                                            cACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGGTGCTTGATTCACTGGGATAACCCTGC‑TTTAACCTACGCCATGCCCGTGATGACGCCAGACAGCGAGAACTTCTTCAGCCCGAACGGTCAGTCGTGa                                                                    >  2:129797/1‑148 (MQ=255)

                                                                              cTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGAtt                                                                  <  2:1892694/148‑1 (MQ=255)

                                                                              cTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGAtt                                                                  <  2:3220011/148‑1 (MQ=255)

                                                                              cTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGAtt                                                                  <  2:1579246/148‑1 (MQ=255)

                                                                                gggATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTAt                                                                <  1:1931711/148‑1 (MQ=255)

                                                                                  gATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCg                                                              <  1:2666425/148‑1 (MQ=255)

                                                                                   aTCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCgg                                                             <  1:3205487/148‑1 (MQ=255)

                                                                                   aTCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCgg                                                             <  1:3204640/148‑1 (MQ=255)

                                                                                     cAGCTTTTTATAGCAACCGGCGCGGGCGCTCGCCCCCGGCCCGTGGTTGTTTGCCGGGGAGAAACCTGC‑GTTTACCTACGCCCAGCCCGGGGTGCCGCCCGACAGCGCGAAGTTATGCAACCCCAACCGTCAGGCGGGATTAtatgtg                                                           >  2:564440/1‑143 (MQ=255)

                                                                                       gCTTTTTATAGCAACCGGCGCGGAAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGcc                                                         >  1:2645496/1‑148 (MQ=255)

                                                                                        cTTTTTATAGCAACCGGCGCGGCCGCTCGACCGCGGCCGTTGCTTGATGCACTGGGATAACGCTGC‑GTTACCCTACGCCAAGCCGGTGATGCCGCCAGACTGCGCGAAGTTCTGCAGCCCGAACGGTCAGTCGGGAGTATAGGTGCCg                                                        >  2:1847069/1‑148 (MQ=255)

                                                                                          ttttATAGCAACCGGCGCGGCCGCGCGCCCCCGGCCGGTGGTTGATGGAAATGGATAACGCAGCTTTTACCAAACGCCA‑GCCCGGGATGGCGCCCCACTGCGAGAACTTCAGCAGCCCGAACCCTCACTCGTGTTTTTAGGTGCCCGa                                                      >  2:3051882/1‑148 (MQ=255)

                                                                                          ttttATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGa                                                      <  2:3276387/148‑1 (MQ=255)

                                                                                             tataGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACt                                                   <  1:654717/148‑1 (MQ=255)

                                                                                              ataGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTa                                                  <  2:1962421/148‑1 (MQ=255)

                                                                                              ataGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTa                                                  <  2:1123035/148‑1 (MQ=255)

                                                                                                aGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTAtt                                                <  1:1892823/148‑1 (MQ=255)

                                                                                                   aaCCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGt                                             <  1:1951476/148‑1 (MQ=255)

                                                                                                   aaCCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGt                                             <  1:1846844/148‑1 (MQ=255)

                                                                                                     ccGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCAGC‑TTTACCCTACGCCAAGCCGGTGATTCCGCCCGACTTCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGCTTATCGGTGCCGGAACTATTGGTCt                                           >  2:2363516/1‑148 (MQ=255)

                                                                                                        gcgcGGCAGCTCGACCGCTGCCGTTGCTTGATTCACTGGGATAACGCTGC‑TTTAACCAACGCCAAGCCGGTGATGCCGCCCGACAGCGAGAAGTTCTGCACCCCCAACGGTCAGTCGTGATTATAGGTGCCGGAACAATTGGTATGGa                                        >  2:3180548/1‑148 (MQ=255)

                                                                                                         cgcgGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGaa                                       <  2:2729287/148‑1 (MQ=255)

                                                                                                            ggCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTg                                    >  2:3169954/1‑148 (MQ=255)

                                                                                                             gCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGCACTATTGGTCTGGAACTgg                                   >  1:2223913/1‑148 (MQ=255)

                                                                                                                 cTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGc                               <  1:3091308/148‑1 (MQ=255)

                                                                                                                 cTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGc                               >  1:381933/1‑148 (MQ=255)

                                                                                                                 cTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGC‑TTTACCCAACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGc                               >  1:3073320/1‑148 (MQ=255)

                                                                                                                      ccGCTGCCGTTGCTTGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAgcg                          <  1:2579453/148‑1 (MQ=255)

                                                                                                                       cGCTGCCGTTGCTTGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAgcgc                         <  1:2714552/148‑1 (MQ=255)

                                                                                                                         cTGCCGTTGCTTGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCa                       <  2:556546/148‑1 (MQ=255)

                                                                                                                         cTGCCGTTGCTTGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCa                       <  2:556181/148‑1 (MQ=255)

                                                                                                                             cGTTGCTTGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCAcgca                   >  1:3123252/1‑148 (MQ=255)

                                                                                                                              gTTGCTTGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCAcgcag                  <  1:2186685/148‑1 (MQ=255)

                                                                                                                               ttGCTTGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGACCTGGCTGCCAGCGCCAcgcagc                 >  2:2025522/1‑148 (MQ=255)

                                                                                                                                tGCTTGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTTGTCTGGAACTGGCTGCCAGCGCCAcgcagcg                >  2:2742518/1‑148 (MQ=255)

                                                                                                                                tGCTTGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCAcgcagcg                >  2:2748260/1‑148 (MQ=255)

                                                                                                                                tGCTTGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCAcgcagcg                >  1:3071183/1‑148 (MQ=255)

                                                                                                                                  cTTGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCAcgcagcgca              >  1:2196484/1‑148 (MQ=255)

                                                                                                                                  cTTGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCAcgcagcgca              >  1:1490408/1‑148 (MQ=255)

                                                                                                                                   ttGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCAcgcagcgcag             <  1:1818040/148‑1 (MQ=255)

                                                                                                                                   ttGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCAcgcagcgcag             <  1:1816781/148‑1 (MQ=255)

                                                                                                                                   ttGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCAcgcagcgcag             <  1:1304776/148‑1 (MQ=255)

                                                                                                                                    tGATGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGa            <  1:2632659/148‑1 (MQ=255)

                                                                                                                                      aTGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATg          >  2:710396/1‑148 (MQ=255)

                                                                                                                                      aTGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATg          >  2:710423/1‑148 (MQ=255)

                                                                                                                                       tGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGt         <  2:3279573/148‑1 (MQ=255)

                                                                                                                                       tGCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGt         <  2:3279500/148‑1 (MQ=255)

                                                                                                                                        gCACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTa        >  1:2586222/1‑148 (MQ=255)

                                                                                                                                         cACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTaa       <  2:317652/148‑1 (MQ=255)

                                                                                                                                         cACTGGGATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTaa       <  2:317687/148‑1 (MQ=255)

                                                                                                                                             gggATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTg   >  2:771664/1‑148 (MQ=255)

                                                                                                                                              ggATAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTCTCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGa  <  1:1515707/148‑1 (MQ=255)

TCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGC‑TTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGA  >  CP009273/2665627‑2665916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGT  >  CP009273/2665631‑2665920

CATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGGAGCTGGCCCCCTGCCGTTGCGTGATGCACTGGGATAACGCTGC                                                                                                                                              >  SRR19973070.209029/1‑150 (MQ=60)

 ATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCCCTGCCGGTTCGTGATGGACTGGGATAACCCTGCT                                                                                                                                             >  SRR19973070.2128195/1‑150 (MQ=60)

  TTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTT                                                                                                                                            <  SRR19973070.3070570/150‑1 (MQ=60)

       GTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTACCC                                                                                                                                       >  SRR19973070.3013615/1‑150 (MQ=60)

             CCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCC                                                                                                                                 <  SRR19973070.1826348/150‑1 (MQ=60)

             CCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCGCGACCCCAGCCGGTGCTTGATGCACTGGGCTAACGCTGCTTTAACCTACGCC                                                                                                                                 >  SRR19973070.1855900/1‑150 (MQ=60)

               ATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCGCGACCCCGGCCCGTGCGTGATGCACTGGGGGAACGCTGCTgttaccgacgccca                                                                                                                               >  SRR19973070.2311328/1‑136 (MQ=60)

                   AAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCG                                                                                                                           <  SRR19973070.512665/150‑1 (MQ=60)

                      CGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTG                                                                                                                        <  SRR19973070.1907405/150‑1 (MQ=60)

                               GCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCA                                                                                                               <  SRR19973070.1146260/150‑1 (MQ=60)

                                   CACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACT                                                                                                           <  SRR19973070.2684833/150‑1 (MQ=60)

                                   CACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACT                                                                                                           <  SRR19973070.2681168/150‑1 (MQ=60)

                                    ACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTG                                                                                                          <  SRR19973070.1768785/150‑1 (MQ=60)

                                    ACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTG                                                                                                          <  SRR19973070.83184/150‑1 (MQ=60)

                                    ACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTG                                                                                                          <  SRR19973070.1462100/150‑1 (MQ=60)

                                             TTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTT                                                                                                 <  SRR19973070.2734170/150‑1 (MQ=60)

                                             TTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTT                                                                                                 <  SRR19973070.2964201/150‑1 (MQ=60)

                                             TTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTT                                                                                                 <  SRR19973070.2964230/150‑1 (MQ=60)

                                             TTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTT                                                                                                 <  SRR19973070.2964276/150‑1 (MQ=60)

                                              TAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTC                                                                                                <  SRR19973070.565044/150‑1 (MQ=60)

                                               AGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCT                                                                                               <  SRR19973070.2543607/150‑1 (MQ=60)

                                                          CCGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGGTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAAC                                                                                    <  SRR19973070.122450/150‑1 (MQ=60)

                                                          ACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAAC                                                                                    <  SRR19973070.1807306/150‑1 (MQ=60)

                                                              CGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTC                                                                                <  SRR19973070.1847001/150‑1 (MQ=60)

                                                                AAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGTCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAG                                                                              <  SRR19973070.1657483/150‑1 (MQ=60)

                                                                   GCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCG                                                                           <  SRR19973070.103863/150‑1 (MQ=60)

                                                                     TGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATTCACTGGGATAACGCTGCGTTTACCAACCCCAAGCCGGTGATTCCGCCAGACAGCCAGAAGTTATGCAGCCCcaaacgtcaggcgtg                                                                         >  SRR19973070.2192539/1‑135 (MQ=60)

                                                                     TGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGCTAACGCTGCGTTACCCTACGCCCAGCCGGTGATTCCGCCAGACAGCGAGAAGTTCGGCAGCCCCAACCGTCAGTCGTG                                                                         >  SRR19973070.2192646/1‑150 (MQ=60)

                                                                            GGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCG                                                                  <  SRR19973070.1944715/150‑1 (MQ=60)

                                                                              GATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGT                                                                <  SRR19973070.2683746/150‑1 (MQ=60)

                                                                               ATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTG                                                               <  SRR19973070.3226222/150‑1 (MQ=60)

                                                                               ATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTG                                                               <  SRR19973070.3225369/150‑1 (MQ=60)

                                                                                 CAGCTTTTTATAGCAACCGGCGCGGAAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCC                                                             >  SRR19973070.2662616/1‑150 (MQ=60)

                                                                                 CAGCTTTTTATAGCAACCGGCGCGGAAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCC                                                             >  SRR19973070.2662727/1‑150 (MQ=60)

                                                                                         TATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTAT                                                     <  SRR19973070.659385/150‑1 (MQ=60)

                                                                                            AGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGG                                                  <  SRR19973070.1905655/150‑1 (MQ=60)

                                                                                               AACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCT                                               <  SRR19973070.1859510/150‑1 (MQ=60)

                                                                                               AACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCT                                               <  SRR19973070.1964588/150‑1 (MQ=60)

                                                                                                     CGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACT                                         <  SRR19973070.1473787/150‑1 (MQ=60)

                                                                                                       CGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGCACTATTGGTCTGGAACTGG                                       >  SRR19973070.2238515/1‑150 (MQ=60)

                                                                                                       CGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGG                                       >  SRR19973070.2552107/1‑150 (MQ=60)

                                                                                                        GGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGC                                      >  SRR19973070.56633/1‑150 (MQ=60)

                                                                                                        GGCAGCTCGACCGCTGCCGTTGCTTGATGCACAGGGAAAACGCTTCTTTAACCTACGCCATTCCGTTGAAGCCCCCAGACTGCGAGAAGTTATTCAGCCCGAACGGTCAGTCGTGATTATCGTTGACGGAACAATTGGTCTGGAACTGGC                                      >  SRR19973070.3227143/1‑150 (MQ=60)

                                                                                                           AGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGC                                   >  SRR19973070.269563/1‑150 (MQ=60)

                                                                                                           AGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGC                                   >  SRR19973070.384737/1‑150 (MQ=60)

                                                                                                           AGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCAACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGC                                   >  SRR19973070.3093052/1‑150 (MQ=60)

                                                                                                             CTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCA                                 <  SRR19973070.3111205/150‑1 (MQ=60)

                                                                                                                  CCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCC                            <  SRR19973070.2596315/150‑1 (MQ=60)

                                                                                                                   CGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCA                           <  SRR19973070.2732088/150‑1 (MQ=60)

                                                                                                                       GCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCA                       >  SRR19973070.3143413/1‑150 (MQ=60)

                                                                                                                          GTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCG                    >  SRR19973070.3090891/1‑150 (MQ=60)

                                                                                                                          GTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCG                    <  SRR19973070.2201139/150‑1 (MQ=60)

                                                                                                                            TGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCA                  >  SRR19973070.3150003/1‑150 (MQ=60)

                                                                                                                            TGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCA                  >  SRR19973070.2211085/1‑150 (MQ=60)

                                                                                                                            TGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCA                  >  SRR19973070.2210979/1‑150 (MQ=60)

                                                                                                                            TGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCA                  >  SRR19973070.1500840/1‑150 (MQ=60)

                                                                                                                               TTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGAT               <  SRR19973070.1830569/150‑1 (MQ=60)

                                                                                                                               TTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGAT               <  SRR19973070.1829302/150‑1 (MQ=60)

                                                                                                                               TTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGAT               <  SRR19973070.1313839/150‑1 (MQ=60)

                                                                                                                                TGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATG              <  SRR19973070.2649825/150‑1 (MQ=60)

                                                                                                                                  ATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTA            >  SRR19973070.2603118/1‑150 (MQ=60)

                                                                                                                                          GGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTCTCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACA    <  SRR19973070.1526331/150‑1 (MQ=60)

                                                                                                                                          GGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACGATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACA    >  SRR19973070.69308/1‑150 (MQ=60)

                                                                                                                                            ATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGT  >  SRR19973070.899489/1‑150 (MQ=60)

CATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGT  >  CP009273/2665631‑2665920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`