Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F488 I0 R1	1275	300.1	10328444	91.0%	9398884	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,665,772	G→T	100%	E123* (GAA→TAA)	hcaD →	phenylpropionate dioxygenase, ferredoxin reductase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,665,772	0	G	T	98.4%	774.9 / 1.7	250	E123* (GAA→TAA)	hcaD	phenylpropionate dioxygenase, ferredoxin reductase subunit
Reads supporting (aligned to +/- strand): ref base G (2/2); new base T (110/135); total (113/137)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.52e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 251 total aligned reads displayed.

CATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTG  >  CP009273/2665625‑2665921

 aTCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAAc                                                                                                                                                    >  1:1661065/1‑150 (MQ=255)

  tCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACg                                                                                                                                                   <  1:2353357/150‑1 (MQ=255)

    tGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACgct                                                                                                                                                 <  2:2001905/150‑1 (MQ=255)

      gaTTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCCCTGGGATAACgctgc                                                                                                                                               >  2:699628/2‑150 (MQ=255)

      cATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCCGCTCGACCGCTGCCGGTGCTTGATGCACAGGGATAACgctgc                                                                                                                                               >  1:4888751/1‑150 (MQ=255)

       aTTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCGCGACCGCTGCCGTTGCTTGTTTCACTGGGGTAACgctgcg                                                                                                                                              >  1:627918/1‑149 (MQ=255)

        ttCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCtt                                                                                                                                             <  1:3451387/150‑1 (MQ=255)

        ttCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCtt                                                                                                                                             >  1:5142941/1‑150 (MQ=255)

        ttCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCtt                                                                                                                                             >  1:4303734/1‑150 (MQ=255)

         tCCGGTGTAACCATCAAACCGCTGGGTCGAGACACACGAGAGTTAGTGTTACCCCACGGCGAAAGCTGGCACTGTGATCAGCTTTTTATCGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCttt                                                                                                                                            <  2:2974680/150‑1 (MQ=255)

            ggTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCCCTGCCGGTGCTTGATGCACAGGGATAAAGCGGCGTTAcc                                                                                                                                         >  1:3043769/1‑150 (MQ=255)

              tgtAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCt                                                                                                                                       >  1:667993/1‑150 (MQ=255)

               gtAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTa                                                                                                                                      <  2:1798152/150‑1 (MQ=255)

                 aaCCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTTCTTGATGCACTGGGATAAAGCTGCTTTACCCAACg                                                                                                                                    >  1:1635007/1‑150 (MQ=255)

                 aaCCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCGGCTTTACCCTACg                                                                                                                                    >  1:775646/1‑150 (MQ=255)

                      tCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATg                                                                                                                               <  2:2305436/150‑1 (MQ=255)

                      tCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCGTTACCCTACGCCATg                                                                                                                               >  2:4349951/1‑150 (MQ=255)

                       cAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGc                                                                                                                              <  1:280408/150‑1 (MQ=255)

                        aaaaCGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGcc                                                                                                                             <  1:3375749/150‑1 (MQ=255)

                        aaaaCGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTAACCGACCCCAAGcc                                                                                                                             >  1:2269929/1‑150 (MQ=255)

                         aaaCGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCg                                                                                                                            <  1:4796620/150‑1 (MQ=255)

                          aaCGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCgg                                                                                                                           >  2:838478/1‑150 (MQ=255)

                            cGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCGCGACCGCGGCCGTTGCTTGATGCACTTGGATAACGCTGCTTTACCCTACGCCATGCCGGTg                                                                                                                         >  1:141199/1‑150 (MQ=255)

                             gCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGa                                                                                                                        <  1:3652980/150‑1 (MQ=255)

                               tGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATg                                                                                                                      <  2:344686/150‑1 (MQ=255)

                               tGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATg                                                                                                                      <  2:5114047/150‑1 (MQ=255)

                                gggTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCGTTACCCTACGCCATGCCGGTGATgc                                                                                                                     >  1:1276714/1‑150 (MQ=255)

                                gggTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGGTGCTTGATGCACTGGGCTAACGCTGCTTTACCCTACGCCATGCCGGTGATgc                                                                                                                     >  1:274342/1‑150 (MQ=255)

                                 ggTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATgcc                                                                                                                    <  1:4218858/150‑1 (MQ=255)

                                 ggTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATgcc                                                                                                                    <  2:1592148/150‑1 (MQ=255)

                                    cgcgACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGGTTTATGCACGGGGATAAAGCTGCTTTACCCAACGCCCTGCCCGGGATtccgcc                                                                                                                 >  1:3982773/1‑150 (MQ=255)

                                    cgcgACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATgccgcc                                                                                                                 <  1:1348317/150‑1 (MQ=255)

                                     gcgACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCa                                                                                                                <  2:4491993/150‑1 (MQ=255)

                                         cacacGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACt                                                                                                            <  1:442424/150‑1 (MQ=255)

                                         cacacGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACt                                                                                                            <  1:3623217/150‑1 (MQ=255)

                                          acacGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTg                                                                                                           >  1:3307819/1‑150 (MQ=255)

                                            acGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCCGCTCGAACGCAGCCGTTGCTTGATGCACTGGGATAACGCTGCATTACCCTACCCCAAGCCGGTGATGCCGCCAGACAGcc                                                                                                         >  2:1362230/1‑149 (MQ=255)

                                               agagTGAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCgaga                                                                                                      <  2:3960653/150‑1 (MQ=255)

                                                 agTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTTCTTTATGCACTGGGATAAAGCTGCTTTACCCTACGCCAAGCCGGTGATGCCGCCAGACAGCGAGAAg                                                                                                    >  1:3794879/1‑150 (MQ=255)

                                                  gTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGt                                                                                                   <  1:2148712/150‑1 (MQ=255)

                                                   ttAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGtt                                                                                                  <  2:4445524/150‑1 (MQ=255)

                                                    tAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCGGCCGTTGCGTGATGCACTGGGATAACGCTGCTTTACCCTACGCCAAGCCGGTGATGCCGCCAGACTGCGAGAAGTTc                                                                                                 >  2:2270182/1‑150 (MQ=255)

                                                     aGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCt                                                                                                <  2:2424731/150‑1 (MQ=255)

                                                     aGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCt                                                                                                <  2:2452643/150‑1 (MQ=255)

                                                     aGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCt                                                                                                <  1:189015/150‑1 (MQ=255)

                                                     aGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCt                                                                                                <  1:1352766/150‑1 (MQ=255)

                                                      gtgtTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGGTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTg                                                                                               <  2:1084137/150‑1 (MQ=255)

                                                      gtgtTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCCCGGCCGGTGCTTGTTGCACTGGGATAACGCTGCTTTACCCAACCCCAAGCCGGGGCTGCCGCCCGACTGCGAGAAGTTCTg                                                                                               >  1:1828434/1‑150 (MQ=255)

                                                       tgtTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGc                                                                                              <  2:3608622/150‑1 (MQ=255)

                                                          tAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGc                                                                                           <  1:2618505/150‑1 (MQ=255)

                                                             ccAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCg                                                                                        <  1:1024041/150‑1 (MQ=255)

                                                              cAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGGTTGATTCACTGGGATAACGCAGCTTTACCCTACGCCAAGCCGGTGATGCCGCCAGACAGCGAGAAGTTCTGCAGCCCgc                                                                                       >  1:2736785/1‑149 (MQ=255)

                                                              cAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGa                                                                                       >  2:305259/1‑150 (MQ=255)

                                                                 cGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACg                                                                                    <  2:5936/150‑1 (MQ=255)

                                                                   gCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGt                                                                                  <  1:2862421/150‑1 (MQ=255)

                                                                    cGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTc                                                                                 >  2:4768678/1‑150 (MQ=255)

                                                                    cGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTc                                                                                 <  2:4747125/150‑1 (MQ=255)

                                                                      aaaGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAg                                                                               >  2:726803/1‑150 (MQ=255)

                                                                      aaaGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCACCTCGACCGCTGCCGTTGCTTGATGCCAGGGGAAAACGCTGCGTTACCCTACGCCATGCCCGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAg                                                                               >  2:3168039/1‑150 (MQ=255)

                                                                       aaGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTTATTCACTGGGATAACGCGGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGt                                                                              >  1:295322/1‑150 (MQ=255)

                                                                       aaGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCAAGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGt                                                                              >  2:3135717/1‑150 (MQ=255)

                                                                           tGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTTCCGTTGCTTGATGCACTGGGGTAACGCTGCTTTACCCTACGCCATGCCGGTGTTGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTg                                                                          >  1:851666/1‑150 (MQ=255)

                                                                              cACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGAtt                                                                       <  2:2700935/150‑1 (MQ=255)

                                                                               aCTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTa                                                                      <  1:411301/150‑1 (MQ=255)

                                                                               aCTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCAGCCGTTTCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCCGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTa                                                                      >  2:1680569/1‑150 (MQ=255)

                                                                                cTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTAt                                                                     <  1:708595/150‑1 (MQ=255)

                                                                                  gggATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGGTGCTTGATGCACAGGGATAACGCTGCTTTAACCAACGCCAAGCCGGTGATGCCGCCAGACAGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCg                                                                   >  1:3405873/1‑150 (MQ=255)

                                                                                   ggATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCCGTCGTGATTATCgg                                                                  >  2:5078299/1‑150 (MQ=255)

                                                                                   ggATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCgg                                                                  <  1:4607872/150‑1 (MQ=255)

                                                                                       cAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTAGTGTTTATCGGTGcc                                                              >  2:4143901/1‑150 (MQ=255)

                                                                                              gtctaGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTa                                                       <  2:4672844/147‑1 (MQ=255)

                                                                                               tataGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCTTGGCGGTGATGCCGCCAGTATGCGAGATTTTCTGCAGCCCTAACGTTCAGTCGTGTTTATCGGTGCCGGAACTAt                                                      <  2:1519905/150‑1 (MQ=255)

                                                                                                ataGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCAAGCCGGTGATTCCGCCAGACAGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATAGGTGCCGGAACTAtt                                                     >  1:2564067/1‑150 (MQ=255)

                                                                                                 taGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTg                                                    <  2:577121/150‑1 (MQ=255)

                                                                                                  aGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTgg                                                   <  1:3260868/150‑1 (MQ=255)

                                                                                                   gCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACGGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGTACTATTGGt                                                  >  2:2761228/1‑150 (MQ=255)

                                                                                                     aaCCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCt                                                >  2:3004598/1‑150 (MQ=255)

                                                                                                        cGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGa                                             <  1:1963678/150‑1 (MQ=255)

                                                                                                             cgGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTgg                                        >  2:1074392/1‑150 (MQ=255)

                                                                                                               gCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCt                                      >  1:1230832/1‑150 (MQ=255)

                                                                                                               gCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCt                                      <  2:2513912/150‑1 (MQ=255)

                                                                                                                cAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTg                                     <  2:2718340/150‑1 (MQ=255)

                                                                                                                 aGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGc                                    >  2:918100/1‑150 (MQ=255)

                                                                                                                  gCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGcc                                   <  1:3612590/150‑1 (MQ=255)

                                                                                                                   cTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCa                                  <  1:3073214/150‑1 (MQ=255)

                                                                                                                   cTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCa                                  >  2:1898708/1‑150 (MQ=255)

                                                                                                                   cTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCa                                  <  2:4912629/150‑1 (MQ=255)

                                                                                                                     cGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAgc                                >  2:2549438/1‑150 (MQ=255)

                                                                                                                         cGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTTCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCa                            <  1:1115572/150‑1 (MQ=255)

                                                                                                                          gCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCAc                           >  1:1546505/1‑150 (MQ=255)

                                                                                                                             gCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACAGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCAcgca                        <  2:1661065/150‑1 (MQ=255)

                                                                                                                              ccGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCAcgcag                       <  2:2597375/150‑1 (MQ=255)

                                                                                                                                       gATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGt              >  2:2609002/1‑150 (MQ=255)

                                                                                                                                       gATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGt              >  2:642426/1‑150 (MQ=255)

                                                                                                                                       gATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGt              >  2:3228386/1‑150 (MQ=255)

                                                                                                                                         tGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTaa            <  2:2842783/150‑1 (MQ=255)

                                                                                                                                            aCTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGt         >  2:4179059/1‑150 (MQ=255)

                                                                                                                                              tGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGa       >  2:1316876/1‑150 (MQ=255)

                                                                                                                                              tGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGa       >  1:2718844/1‑150 (MQ=255)

                                                                                                                                               gggATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGAc      >  2:246806/1‑150 (MQ=255)

CATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTG  >  CP009273/2665625‑2665921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAG  >  CP009273/2665623‑2665919

TTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAT                                                                                                                                                     >  SRR19973063.2605632/1‑150 (MQ=60)

TTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAT                                                                                                                                                     <  SRR19973063.2914511/150‑1 (MQ=60)

  CATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCGTGTTTCACTGGGATAA                                                                                                                                                   >  SRR19973063.882292/1‑150 (MQ=60)

  CATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTAGACCGCTGCCGTTGCTTGATGCACTGGGATAA                                                                                                                                                   >  SRR19973063.590400/1‑150 (MQ=60)

   ATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAAC                                                                                                                                                  >  SRR19973063.1666127/1‑150 (MQ=60)

    TCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACG                                                                                                                                                 <  SRR19973063.2360889/150‑1 (MQ=60)

    TCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACG                                                                                                                                                 <  SRR19973063.2360901/150‑1 (MQ=60)

    TCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCAGCCGTTGCTTTATGCACAGGGATAACG                                                                                                                                                 >  SRR19973063.2465401/1‑150 (MQ=60)

       GCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTG                                                                                                                                              <  SRR19973063.4786214/150‑1 (MQ=60)

        CATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCCGCTCGACCGCTGCCGGTGCTTGATGCACAGGGATAACGCTGC                                                                                                                                             >  SRR19973063.4904523/1‑150 (MQ=60)

         ATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGGTGCGTGATGCACAGGGATAACGCTGCG                                                                                                                                            >  SRR19973063.4897877/1‑150 (MQ=60)

         ATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCGCGACCGCTGCCGTTGCTTGTTTCACTGGGGTAACGCTGCG                                                                                                                                            >  SRR19973063.629766/1‑150 (MQ=60)

          TTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTT                                                                                                                                           <  SRR19973063.3462305/150‑1 (MQ=60)

          TTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTT                                                                                                                                           <  SRR19973063.3462360/150‑1 (MQ=60)

          TTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTT                                                                                                                                           >  SRR19973063.5159492/1‑150 (MQ=60)

          TTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTT                                                                                                                                           >  SRR19973063.4317408/1‑150 (MQ=60)

          TTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCCGTGCTTGTTGCACTGGGATAACGCTGCTT                                                                                                                                           >  SRR19973063.693009/1‑150 (MQ=60)

           TCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTT                                                                                                                                          <  SRR19973063.3939764/150‑1 (MQ=60)

                TGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCT                                                                                                                                     >  SRR19973063.669946/1‑150 (MQ=60)

                TGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCGTGATGCACTGGGATAAAGCTGCTTTACCCA                                                                                                                                     >  SRR19973063.3705939/1‑150 (MQ=60)

                  TAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTAC                                                                                                                                   <  SRR19973063.3370241/150‑1 (MQ=60)

                   AACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTTCTTGATGCACTGGGATAAAGCTGCTTTACCCAACG                                                                                                                                  >  SRR19973063.1640005/1‑150 (MQ=60)

                   AACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACG                                                                                                                                  <  SRR19973063.1679401/150‑1 (MQ=60)

                   AACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCGGCTTTACCCTACG                                                                                                                                  >  SRR19973063.777900/1‑150 (MQ=60)

                   AACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCGGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACG                                                                                                                                  >  SRR19973063.777932/1‑150 (MQ=60)

                       ATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCAT                                                                                                                              <  SRR19973063.1913780/150‑1 (MQ=60)

                       ATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCAT                                                                                                                              <  SRR19973063.1913789/150‑1 (MQ=60)

                       ATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCAT                                                                                                                              <  SRR19973063.1322519/150‑1 (MQ=60)

                       ATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCAT                                                                                                                              <  SRR19973063.1320195/150‑1 (MQ=60)

                         CAACACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCGTTTTATAGCCACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGC                                                                                                                            <  SRR19973063.4052456/150‑1 (MQ=60)

                         CAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGC                                                                                                                            <  SRR19973063.281240/150‑1 (MQ=60)

                         CAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGC                                                                                                                            <  SRR19973063.281668/150‑1 (MQ=60)

                          AAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTAaccgaccccaagcc                                                                                                                           >  SRR19973063.2277173/1‑136 (MQ=60)

                          AAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCC                                                                                                                           <  SRR19973063.3386503/150‑1 (MQ=60)

                           AAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCG                                                                                                                          <  SRR19973063.4811948/150‑1 (MQ=60)

                              CGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCGCGACCGCGGCCGTTGCTTGATGCACTTGGATAACGCTGCTTTACCCTACGCCATGCCGGTG                                                                                                                       >  SRR19973063.141639/1‑150 (MQ=60)

                               GCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGA                                                                                                                      <  SRR19973063.3664692/150‑1 (MQ=60)

                               GCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCAACGCCATGCCGGTGA                                                                                                                      >  SRR19973063.1223035/1‑150 (MQ=60)

                                CTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGGTGCTTGATGCACTGGGATAACGCTGCTTTACCCGACGCCATGCCGGTGAT                                                                                                                     >  SRR19973063.1665923/1‑150 (MQ=60)

                                  GGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCGTTACCCTACGCCATGCCGGTGATGC                                                                                                                   >  SRR19973063.1280518/1‑150 (MQ=60)

                                  GGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGGTGCTTGATGCACTGGGCTAACGCTGCTTTACCCTACGCCATGCCGGTGATGC                                                                                                                   >  SRR19973063.275158/1‑150 (MQ=60)

                                   GGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCC                                                                                                                  <  SRR19973063.4232308/150‑1 (MQ=60)

                                    GTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCCGCTCGACCCCAGCCCGTGCTTGTTGCACTGGGATAAAGCTGCTTTAaccaaagcccagccggtgattccg                                                                                                                 >  SRR19973063.2348846/1‑126 (MQ=60)

                                      CGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGGTTTATGCACGGGGATAAAGCTGCTTTACCCAACGCCCTGCCCGGGATTCCGCC                                                                                                               >  SRR19973063.3995536/1‑150 (MQ=60)

                                      CGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCC                                                                                                               <  SRR19973063.1052955/150‑1 (MQ=60)

                                      CGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCC                                                                                                               <  SRR19973063.1352365/150‑1 (MQ=60)

                                        CGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGGTGCACTGGGATAACGCAGCTTTACCCTACGCCAAGCCGGTGATGCCGCCAG                                                                                                             >  SRR19973063.1218499/1‑150 (MQ=60)

                                          ACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCGGCCGTTGCTTGATGCACTGGGCTAACGCTGCTTTTCCCAACGCCATGCCGGTGATGCCGCCAGAC                                                                                                           >  SRR19973063.2177086/1‑150 (MQ=60)

                                           CACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACT                                                                                                          <  SRR19973063.3018717/150‑1 (MQ=60)

                                           CACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACT                                                                                                          <  SRR19973063.3634844/150‑1 (MQ=60)

                                           CACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACT                                                                                                          <  SRR19973063.3634886/150‑1 (MQ=60)

                                           CACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACT                                                                                                          <  SRR19973063.1404738/150‑1 (MQ=60)

                                           CACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACT                                                                                                          <  SRR19973063.443664/150‑1 (MQ=60)

                                           CACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACT                                                                                                          <  SRR19973063.3634779/150‑1 (MQ=60)

                                            ACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCAACGCCAAGCCGGTGATGCCGCCAGACTG                                                                                                         >  SRR19973063.3318400/1‑150 (MQ=60)

                                               CGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATTCACTGGGATAACGCTGCTTTAACCAACGCCATGCCGGTGATGCCGCCAGACTGCGA                                                                                                      >  SRR19973063.318227/1‑150 (MQ=60)

                                                  GAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAA                                                                                                   <  SRR19973063.4173203/150‑1 (MQ=60)

                                                   AGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTTCTTTATGCACTGGGATAAAGCTGCTTTACCCTACGCCAAGCCGGTGATGCCGCCAGACAGCGAGAAG                                                                                                  >  SRR19973063.3806977/1‑150 (MQ=60)

                                                   AGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCGGCCGTTGCTTGATGCACGGGGATAACGCGGCTTTAACCAACGCCCAGCCGGGGATGCCGCCAGACAGCGAGAAC                                                                                                  >  SRR19973063.3081586/1‑150 (MQ=60)

                                                    GTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGT                                                                                                 <  SRR19973063.2155443/150‑1 (MQ=60)

                                                       AGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCT                                                                                              <  SRR19973063.189596/150‑1 (MQ=60)

                                                       AGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCT                                                                                              <  SRR19973063.1356828/150‑1 (MQ=60)

                                                       AGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCT                                                                                              <  SRR19973063.1356710/150‑1 (MQ=60)

                                                        GTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATTCACTGGGCTAACCCTGCTTTTACCTACGCCAAGCCGGTGATTCCGCCCCACAGCGAGAAGTTATG                                                                                             >  SRR19973063.1895395/1‑150 (MQ=60)

                                                        GTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTG                                                                                             <  SRR19973063.2823853/150‑1 (MQ=60)

                                                        GTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCCCGGCCGGTGCTTGTTGCACTGGGATAACGCTGCTTTACCCAACCCCAAGCCGGGGCTGCCGCCCGACTGCGAGAAGTTCTG                                                                                             >  SRR19973063.1834124/1‑150 (MQ=60)

                                                            TAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGC                                                                                         <  SRR19973063.2626841/150‑1 (MQ=60)

                                                               CCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCG                                                                                      <  SRR19973063.1027130/150‑1 (MQ=60)

                                                               CCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCG                                                                                      <  SRR19973063.3616301/150‑1 (MQ=60)

                                                               CCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCG                                                                                      <  SRR19973063.1598148/150‑1 (MQ=60)

                                                               CCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCG                                                                                      <  SRR19973063.4413726/150‑1 (MQ=60)

                                                                CAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGGTTGATTCACTGGGATAACGCAGCTTTACCCTACGCCAAGCCGGTGATGCCGCCAGACAGCGAGAAGTTCTGCAGCCCGC                                                                                     >  SRR19973063.2745620/1‑150 (MQ=60)

                                                                CAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCGGCCGTTGCTTGATGCACTGGGGTAACGCTGCTTTAACCTACGCCAAGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGA                                                                                     >  SRR19973063.2745865/1‑150 (MQ=60)

                                                                CAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCCACCCCTGCCGGTGCTTGATGCACAGGGATAACCCAGCTTTAACCTACGCCAAGCCGGGGATGCCGCCAGACAGCGAGAAGTTCTGCAGCCCGA                                                                                     >  SRR19973063.2745857/1‑150 (MQ=60)

                                                                  ACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAAC                                                                                   <  SRR19973063.840902/150‑1 (MQ=60)

                                                                   CGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACG                                                                                  <  SRR19973063.4244282/150‑1 (MQ=60)

                                                                     GCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGT                                                                                <  SRR19973063.2871592/150‑1 (MQ=60)

                                                                      CGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCGCGAACCCTTCCGTTGGTTGATGCACTGGGATAAAGCAGCTTTAACCAACGCCATTCCGGGGATGCCGCCAGACAGCGAGAAATTATGCCGCCCGCACGGTC                                                                               >  SRR19973063.4650696/1‑150 (MQ=60)

                                                                       GAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCA                                                                              <  SRR19973063.2811322/150‑1 (MQ=60)

                                                                       GAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCA                                                                              <  SRR19973063.4515114/150‑1 (MQ=60)

                                                                       GAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCA                                                                              <  SRR19973063.56375/150‑1 (MQ=60)

                                                                       GAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCCCTGCCCTTGCTTGATGGACGTGGATAAAGCTGCTTTTACCTACGCCATTCCGGTGATGCCGCCAGACTGCGATAAATTCTGCAGCCCGAACGGTCA                                                                              >  SRR19973063.2100332/1‑150 (MQ=60)

                                                                         AAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTTATTCACTGGGATAACGCGGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGT                                                                            >  SRR19973063.296194/1‑150 (MQ=60)

                                                                          AGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTC                                                                           <  SRR19973063.2693013/150‑1 (MQ=60)

                                                                            CTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCGGCCGGTGCTTGATGCACAGGGATAACGCTGCTTTACCCAACGCCAAGCCGGTGATTCCGCCAGACATCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGT                                                                         >  SRR19973063.340623/1‑150 (MQ=60)

                                                                             TGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGGAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGGTTTAACCTACGCCATGCCGGGGATGCCGCCAGACTGCGAGAAGTTATGCAGCCCCAACGGTCAGTCGGG                                                                        >  SRR19973063.530008/1‑150 (MQ=60)

                                                                             TGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTTCCGTTGCTTGATGCACTGGGGTAACGCTGCTTTACCCTACGCCATGCCGGTGTTGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTG                                                                        >  SRR19973063.854123/1‑150 (MQ=60)

                                                                             TGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCCTTGGTTTATGCACGGGGATAACCCTGCTTTAaccaacccccagcccgtgttgccgcccgacagcgagaagttcatccgccccaaacggcagtgggg                                                                        >  SRR19973063.530156/1‑85 (MQ=60)

                                                                               GCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGAT                                                                      <  SRR19973063.1253726/150‑1 (MQ=60)

                                                                                 ACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTA                                                                    <  SRR19973063.412457/150‑1 (MQ=60)

                                                                                  CTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTAT                                                                   <  SRR19973063.710634/150‑1 (MQ=60)

                                                                                  CTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTAT                                                                   <  SRR19973063.710678/150‑1 (MQ=60)

                                                                                   TGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATC                                                                  <  SRR19973063.3499218/150‑1 (MQ=60)

                                                                                   TGGGATCAGCTTTTTATAGCAACCGGCGCGGCACCACGACCGCTGCCCGTTCGTGTATCACTGGGATAACCCTGCTTTACCCAACTCCATGCCGGTGATGCCGCCccaaagcgagaaggtatgcagaccgaacggtcacgcgtgcttaga                                                                  >  SRR19973063.2078848/1‑105 (MQ=31)

                                                                                    GGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGGTGCTTGATGCACAGGGATAACGCTGCTTTAACCAACGCCAAGCCGGTGATGCCGCCAGACAGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCG                                                                 >  SRR19973063.3416706/1‑150 (MQ=60)

                                                                                     GGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGG                                                                <  SRR19973063.4622609/150‑1 (MQ=60)

                                                                                          AGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTTCCCTACGCCATGCCGGGGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCG                                                           >  SRR19973063.3439129/1‑150 (MQ=60)

                                                                                                 TATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTAT                                                    <  SRR19973063.1470703/150‑1 (MQ=60)

                                                                                                  ATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCAAGCCGGTGATTCCGCCAGACAGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATAGGTGCCGGAACTATT                                                   >  SRR19973063.2572236/1‑150 (MQ=60)

                                                                                                    AGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGG                                                 <  SRR19973063.3271337/150‑1 (MQ=60)

                                                                                                       AACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCT                                              <  SRR19973063.287543/150‑1 (MQ=60)

                                                                                                        ACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTG                                             <  SRR19973063.740218/150‑1 (MQ=60)

                                                                                                         CCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACAGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGG                                            >  SRR19973063.3760305/1‑150 (MQ=60)

                                                                                                         CCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTAACCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGG                                            >  SRR19973063.42704/1‑150 (MQ=60)

                                                                                                          CGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGA                                           <  SRR19973063.1969824/150‑1 (MQ=60)

                                                                                                             CGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACT                                        >  SRR19973063.4568115/1‑150 (MQ=60)

                                                                                                              GCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTG                                       <  SRR19973063.1366331/150‑1 (MQ=60)

                                                                                                                GGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGC                                     >  SRR19973063.2730500/1‑150 (MQ=60)

                                                                                                                GGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCCGGAACTGGC                                     >  SRR19973063.1769663/1‑150 (MQ=60)

                                                                                                                 GCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCT                                    >  SRR19973063.1234509/1‑150 (MQ=60)

                                                                                                                   AGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGACCTGGCTGC                                  >  SRR19973063.4073296/1‑150 (MQ=60)

                                                                                                                    GCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCC                                 <  SRR19973063.3624188/150‑1 (MQ=60)

                                                                                                                    GCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCC                                 <  SRR19973063.3624272/150‑1 (MQ=60)

                                                                                                                     CTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCA                                <  SRR19973063.3083086/150‑1 (MQ=60)

                                                                                                                          CCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCC                           >  SRR19973063.2583153/1‑150 (MQ=60)

                                                                                                                          CCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCC                           <  SRR19973063.209813/150‑1 (MQ=60)

                                                                                                                           CGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTTCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCA                          <  SRR19973063.1118966/150‑1 (MQ=60)

                                                                                                                           CGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCA                          <  SRR19973063.3379068/150‑1 (MQ=60)

                                                                                                                            GCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCAC                         >  SRR19973063.1551245/1‑150 (MQ=60)

                                                                                                                             CTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACA                        <  SRR19973063.310152/150‑1 (MQ=60)

                                                                                                                               GCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCA                      <  SRR19973063.5118396/150‑1 (MQ=60)

                                                                                                                                 CGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGC                    >  SRR19973063.1618799/1‑150 (MQ=60)

                                                                                                                                    TGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCA                 >  SRR19973063.4813530/1‑150 (MQ=60)

                                                                                                                                      CTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGA               >  SRR19973063.2987784/1‑150 (MQ=60)

                                                                                                                                       TTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGAT              >  SRR19973063.222408/1‑150 (MQ=60)

                                                                                                                                          ATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTA           >  SRR19973063.3285306/1‑150 (MQ=60)

                                                                                                                                               CTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTG      <  SRR19973063.651837/150‑1 (MQ=60)

                                                                                                                                                TGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGA     >  SRR19973063.2727381/1‑150 (MQ=60)

                                                                                                                                                TGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGA     >  SRR19973063.2727624/1‑150 (MQ=60)

                                                                                                                                                   GATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAG  <  SRR19973063.2322823/150‑1 (MQ=60)

TTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAG  >  CP009273/2665623‑2665919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`