Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,665,772 G→T 100% E123* (GAA→TAA)  hcaD → phenylpropionate dioxygenase, ferredoxin reductase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,665,7720GT99.8% 731.4 / ‑6.5 238E123* (GAA→TAA) hcaDphenylpropionate dioxygenase, ferredoxin reductase subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (113/123);  minor base A (2/0);  total (115/123)
Fisher's exact test for biased strand distribution p-value = 2.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 239 total aligned reads displayed.

TTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACA  >  CP009273/2665623‑2665918
                                                                                                                                                     |                                                                                                                                                   
  cATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCCGCGCGACCCCTGCCGTTGCTTGTTTCACAGGGATaa                                                                                                                                                   >  1:4726669/1‑150 (MQ=255)
  cATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATaa                                                                                                                                                   <  1:4384526/150‑1 (MQ=255)
   aTCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAAc                                                                                                                                                  <  1:1786147/150‑1 (MQ=255)
      tGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACgct                                                                                                                                               <  1:4412484/150‑1 (MQ=255)
      tGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACgct                                                                                                                                               <  2:4016486/150‑1 (MQ=255)
       gCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACgctg                                                                                                                                              <  2:3034301/150‑1 (MQ=255)
       gCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACGGGGGTAACgctg                                                                                                                                              >  1:2925147/1‑150 (MQ=255)
        cagtCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGGACTGGGATCAGCTTTTTAGAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACgctgc                                                                                                                                             <  2:4327678/147‑1 (MQ=255)
        cATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACgctgc                                                                                                                                             >  2:3454590/1‑150 (MQ=255)
           tCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCttt                                                                                                                                          <  2:567896/150‑1 (MQ=255)
           tCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCttt                                                                                                                                          <  2:5018222/150‑1 (MQ=255)
           tCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCttt                                                                                                                                          <  1:5015291/150‑1 (MQ=255)
            ccGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTa                                                                                                                                         <  1:4666888/150‑1 (MQ=255)
              ggTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCACGACCGCGGCCGTTGCTTGATGCACTGGGGTAAAGCGGCTTTTcc                                                                                                                                       >  1:1703416/1‑150 (MQ=255)
                tgtAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑t                                                                                                                                    <  2:1026906/150‑1 (MQ=255)
                tgtAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑t                                                                                                                                    <  2:3303150/150‑1 (MQ=255)
                 gtAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑Ta                                                                                                                                   <  2:3616484/150‑1 (MQ=255)
                   aaCCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCCGCGCGACCCCAGCCGGTGCGTGATGCACAGGGATAACGCTGCATTACCC‑AAcc                                                                                                                                 >  2:5131388/1‑149 (MQ=255)
                      cATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑AACGCCa                                                                                                                              >  2:2538760/1‑150 (MQ=255)
                      cATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCGGCTTTACCC‑TACGCCa                                                                                                                              >  2:282541/1‑150 (MQ=255)
                       aTCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCCCAGCCGTTGCGTGATGCACTGGGATAACGCTGCGTTACCC‑AACGccca                                                                                                                             >  2:1404696/1‑148 (MQ=255)
                         cAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGc                                                                                                                           >  2:3952810/1‑150 (MQ=255)
                              cGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATACCGGTg                                                                                                                      <  1:2988876/150‑1 (MQ=255)
                               gCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCCTTGCTTGATGCACAGGGATAACGCTGCTTTACCC‑AACGCCATGCCCGTGa                                                                                                                     >  2:5227470/1‑150 (MQ=255)
                               gCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCGCGACCGCTGCCGGTTCTTGATGCACAGGGATTAAGCAGCGTTAACC‑AACGCCATTCCGGTGa                                                                                                                     >  2:2410646/1‑150 (MQ=255)
                                cTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGAt                                                                                                                    <  1:2898831/150‑1 (MQ=255)
                                 tGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCACGACCCCAGCCGGTGCTTGTTGCACAGGGATAAAGCTGCTTTTACC‑AACGCCAGGCCGGGGATg                                                                                                                   >  1:1280852/1‑150 (MQ=255)
                                   ggTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCGGCCGTTGCGTGATGCACGGGGATAACGCTGCGGTAGCC‑GAAGCCCTGCCGGGGCTgcc                                                                                                                 >  2:3657319/1‑150 (MQ=255)
                                    gTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATgccg                                                                                                                <  1:4496430/150‑1 (MQ=255)
                                        cgACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAg                                                                                                            <  2:3786093/150‑1 (MQ=255)
                                        cgACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCGGCCGGTGCTTGATTCACTGGGATAACGCTGGTTTAACC‑AACGCCATGCCGGGGAGgccgcccg                                                                                                            >  1:3723801/1‑148 (MQ=255)
                                           cacacGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACt                                                                                                         <  1:4899426/150‑1 (MQ=255)
                                           cacacGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACt                                                                                                         <  1:2623023/150‑1 (MQ=255)
                                           cacacGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACt                                                                                                         <  1:1621692/150‑1 (MQ=255)
                                              acGAGAGTTAGTGTTAACCAACGGCGACAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCg                                                                                                      <  1:963145/150‑1 (MQ=255)
                                              acGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCGGCCGTTGCATGATGCAATGGGATAACGCAGCGTTACCC‑AACGCCAAGCCGGGGATGCCCCCAAACTGCg                                                                                                      >  1:3537155/1‑150 (MQ=255)
                                                  gagTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCAt                                                                                                                             >  2:3113888/1‑123 (MQ=255)
                                                  gagTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCAt                                                                                                                             <  1:3113888/123‑1 (MQ=255)
                                                    gTTAGTGTCAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGt                                                                                                <  2:4259068/150‑1 (MQ=255)
                                                      tAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTc                                                                                              <  2:3873521/150‑1 (MQ=255)
                                                       aGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATCGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCt                                                                                             <  2:1209085/150‑1 (MQ=255)
                                                        gtgtTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCGCGACCGCTGCCGGTGCGTGATGCACAGGGATAACGCGGCTTTTCCC‑AACGCCATTCCGGGGTTGCCGCCACACTGCGAGAACTTCTg                                                                                            >  2:2696180/1‑150 (MQ=255)
                                                         tgtTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGc                                                                                           <  1:123995/150‑1 (MQ=255)
                                                          gtTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCa                                                                                          <  1:3709558/150‑1 (MQ=255)
                                                          gtTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCACGACCCCTGCCGTTGGGTGATGCACTGGGGTAAAGCAGCGTTACAC‑AACCCCCAGCCGGGGAAGCCGCCCCAAAGCGAGAAGTTCTGCa                                                                                          >  1:613134/1‑150 (MQ=255)
                                                             aaCCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCCCTGCCGGTGCGTGATTCACTGGGATAACGCGGCGGTACCC‑GACGCCATGCCGGGGATGCCGCCCGACAGCGAGAAGTTATGCAGcc                                                                                       >  1:3025500/1‑150 (MQ=255)
                                                              aCCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCCGCTCGACCGCTGCCGTTGCTTGTTTCACTGGGTTAAAGCTGCTTTTCCC‑AACGCCATGCCGGTGGTGCCGCCAGACAGCGAGAAGTTATGCAGccc                                                                                      >  2:1137657/1‑150 (MQ=255)
                                                                cAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGTTAACGCGGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGa                                                                                    >  2:2139534/1‑150 (MQ=255)
                                                                 aaCGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGGCGCGCGACCCCAGCCGTTGCTTGATTGACAGGGTTTAAGCGGCGTTAACC‑AACGCCCAGCCCGTGATGCCGCCCGACAGCCAGAAGTTCAGCAGCCCCaa                                                                                   >  2:5093220/1‑150 (MQ=255)
                                                                 aaCGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCGCGACCGCTGCCGTTTCGTGTTTCACAGGGATAAAGCTGCGTTTACC‑TACCCCCTGCCGGTGATGCCGCCAGACAGCGAGAACTTCAGCAGCCCCaa                                                                                   >  2:1068164/1‑150 (MQ=255)
                                                                   cGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACg                                                                                 <  1:1725665/150‑1 (MQ=255)
                                                                      cGAAAGCAGGCACTGGGATCAGCTTTTTAGAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTc                                                                              <  2:4487817/150‑1 (MQ=255)
                                                                        aaaGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAg                                                                            <  2:1618486/150‑1 (MQ=255)
                                                                        aaaGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAg                                                                            <  2:222151/150‑1 (MQ=255)
                                                                        aaaGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACAGCGAGAAGTTCTGCAGCCCGAACGGTCAg                                                                            >  2:3261290/1‑150 (MQ=255)
                                                                         aaGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGt                                                                           <  1:2527783/150‑1 (MQ=255)
                                                                         aaGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCAGCCGTTGCTTGATGCACTGGGATAAAGCTGCTTTAAAC‑AACGCCAAGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGt                                                                           >  1:4382745/1‑150 (MQ=255)
                                                                         aaGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCCCAGCCGTTGCGTGATTCACGGGGATAACGCAGCTTTACCC‑TACCCCATGCCGGTGGTGCCGCCAGACAGCGAGAAGTTCATCAGCCCGAAAGGTCAGt                                                                           >  2:2098736/1‑150 (MQ=255)
                                                                           gCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCCGCGCGACCGCGGCCGGTGCGTTATTCACTGGGTTAAAGCGGCGTTTCCC‑AACGCCAAGCCGGTGATTCCGCCAGACAGCGAGAAGTTATGCAGCCCGAACGGTCAGGCg                                                                         >  1:1717872/1‑150 (MQ=255)
                                                                           gCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCg                                                                         <  1:860289/150‑1 (MQ=255)
                                                                              ggCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTAGAACGCTGCCGTTGCTTGATTCACTGGGATAACGCTGCTTTACCC‑TACGCCATTCCGGTGATGCCGCCAGACTGCTAGAAGTTCTGCAGCCCGAACGGTCAGTAGTGa                                                                      >  2:871613/1‑150 (MQ=255)
                                                                                  cTGGGATCATCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTAt                                                                  <  1:676210/150‑1 (MQ=255)
                                                                                  cTGGGATCAGCTTTTTATAGCAACCGGCGCGGCCCCGCGACCCCAGCCCGTTCGTGATTCACAGGGATAACCCAGCATTACCCAAACCCCAGCCCGGGGA‑GCCCCCACACAGCCCGAACATCAGCAGCCCCAACCGTCAGTCGTGAatat                                                                  >  2:1926559/1‑150 (MQ=255)
                                                                                  cTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTAt                                                                  <  1:2290542/150‑1 (MQ=255)
                                                                                  cTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTAt                                                                  <  2:2545936/150‑1 (MQ=255)
                                                                                   tGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATc                                                                 <  1:4044227/150‑1 (MQ=255)
                                                                                       aTCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCCCTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTg                                                             <  1:1060400/150‑1 (MQ=255)
                                                                                       aTCAGCTTTTTATAGCAACCGGCGCGGCAGCGCGACCCCAGCCGTTGCGTGATGCCCAGGGATAACGCGGCTTTTACC‑GACGCCCAGCCCGTGATTCCGCCACAAAGCGAGAACTTCTGCAACCCCAACGGGCAGTCGGGATTATCgggg                                                             >  2:4578361/1‑148 (MQ=255)
                                                                                         cAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGcc                                                           <  2:4101968/150‑1 (MQ=255)
                                                                                         cAGCTTTTTATAGCAACCGGCGCGGCAGCACGAACCCAGCCCTTGAATTATGCACTGGGGTAAACCTGCTTTAAAC‑AAACCCAAGACGGGTATTACGCCCCACAACGAGAACTTCTGCACCCCGCACGGTCAATACTGATTATAGGTggc                                                           >  2:5024555/1‑148 (MQ=255)
                                                                                          aGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTTCCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCAACAACTTCTGCACCCCCAACCGTCAGTCGTGAATATCGGTGCCg                                                          >  2:3917843/1‑150 (MQ=255)
                                                                                               tttATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACt                                                     <  1:1510368/150‑1 (MQ=255)
                                                                                                 tataGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTAt                                                   <  1:1617768/150‑1 (MQ=255)
                                                                                                 tataGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTAt                                                   <  1:2488887/150‑1 (MQ=255)
                                                                                                     gCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGt                                               <  1:5208675/150‑1 (MQ=255)
                                                                                                       aaCCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCt                                             <  2:2999106/150‑1 (MQ=255)
                                                                                                        aCCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTg                                            <  1:1732229/150‑1 (MQ=255)
                                                                                                         ccGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTgg                                           <  2:2423128/150‑1 (MQ=255)
                                                                                                               cgGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTgg                                     <  1:2668483/150‑1 (MQ=255)
                                                                                                                ggCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGc                                    <  2:1224565/150‑1 (MQ=255)
                                                                                                                 gCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCt                                   >  1:5270813/1‑150 (MQ=255)
                                                                                                                          ccGCTGCCGGTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGcc                          <  2:4291797/150‑1 (MQ=255)
                                                                                                                            gCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TCCGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCAc                        <  2:3379915/150‑1 (MQ=255)
                                                                                                                            gCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCAc                        <  1:3493819/150‑1 (MQ=255)
                                                                                                                            gCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCAc                        >  1:4612396/1‑150 (MQ=255)
                                                                                                                             cTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCAcg                       <  1:3789047/150‑1 (MQ=255)
                                                                                                                               gCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCAcgca                     >  2:1694029/1‑150 (MQ=255)
                                                                                                                               gCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCAcgca                     <  1:2242831/150‑1 (MQ=255)
                                                                                                                               gCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGAATATCGGTGCCGGAACTATAGGTCTGGAACTGGCTGCAAGCGCCAcgca                     >  1:4788201/1‑150 (MQ=255)
                                                                                                                                ccGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCAcgcag                    >  2:4733439/1‑150 (MQ=255)
                                                                                                                                ccGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCAcgcag                    >  2:1502230/1‑150 (MQ=255)
                                                                                                                                 cGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCAcgcagc                   <  2:1514724/150‑1 (MQ=255)
                                                                                                                                      cTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGACCTGGCTGCCAGCGCCACGCAGCGCAGa              >  1:3182225/1‑150 (MQ=255)
                                                                                                                                      cTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGa              >  2:2901493/1‑150 (MQ=255)
                                                                                                                                          aTGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTa          >  1:2507184/1‑150 (MQ=255)
                                                                                                                                             cACTGGGAAAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTCTTTGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAgg       >  2:1498605/1‑150 (MQ=255)
                                                                                                                                               cTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTg     <  1:5131388/150‑1 (MQ=255)
                                                                                                                                                tGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGa    >  2:70130/1‑150 (MQ=255)
                                                                                                                                                tGGGATAACGCTGATTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACAGCGAGAAGTTCTGCAGCCCGAACTGTCAGTCGAGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGgaga    >  2:828014/1‑150 (MQ=255)
                                                                                                                                                  ggATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACa  >  1:4065141/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACA  >  CP009273/2665623‑2665918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACA  >  CP009273/2665625‑2665918
                                                                                                                                                   |                                                                                                                                                  
CATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAA                                                                                                                                                  <  SRR19973062.4406999/150‑1 (MQ=60)
CATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAA                                                                                                                                                  <  SRR19973062.4406997/150‑1 (MQ=60)
CATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCAGCCGGTGGTTGATTCACTGGGATAA                                                                                                                                                  >  SRR19973062.4751542/1‑150 (MQ=60)
 ATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAAC                                                                                                                                                 <  SRR19973062.1794801/150‑1 (MQ=60)
    TGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCT                                                                                                                                              <  SRR19973062.4435042/150‑1 (MQ=60)
    TGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCT                                                                                                                                              <  SRR19973062.3593225/150‑1 (MQ=60)
     GCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTG                                                                                                                                             <  SRR19973062.2372413/150‑1 (MQ=60)
     GCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACGGGGGTAACGCTG                                                                                                                                             >  SRR19973062.2940555/1‑150 (MQ=60)
     GCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCCCAGCCGTTGCGTGATGCACTGGGATAACGCTG                                                                                                                                             >  SRR19973062.253345/1‑150 (MQ=60)
        TTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTT                                                                                                                                          <  SRR19973062.522938/150‑1 (MQ=60)
         TCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTT                                                                                                                                         <  SRR19973062.5041843/150‑1 (MQ=60)
          CCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTA                                                                                                                                        <  SRR19973062.4691299/150‑1 (MQ=60)
                TAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTAC                                                                                                                                  <  SRR19973062.2125628/150‑1 (MQ=60)
                  ACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCCGCACGACCGCTGCCGTTGCGTGATGCACAGGGCTAACGCAGCTTTACCCAACGC                                                                                                                                >  SRR19973062.315103/1‑150 (MQ=60)
                   CCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCC                                                                                                                               <  SRR19973062.2520149/150‑1 (MQ=60)
                     gagttcagacgtgtgctcttccgatctGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCAT                                                                                                                             <  SRR19973062.2450871/123‑1 (MQ=60)
                     gagttcagacgtgtgctcttccgatctGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCAT                                                                                                                             <  SRR19973062.3129918/123‑1 (MQ=60)
                      TCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATG                                                                                                                            <  SRR19973062.2175727/150‑1 (MQ=60)
                        AAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCC                                                                                                                          <  SRR19973062.537667/150‑1 (MQ=60)
                           ACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGT                                                                                                                       <  SRR19973062.5005510/150‑1 (MQ=60)
                            CGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATACCGGTG                                                                                                                      <  SRR19973062.3004542/150‑1 (MQ=60)
                              CTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGAT                                                                                                                    <  SRR19973062.2914131/150‑1 (MQ=60)
                              CTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGAT                                                                                                                    <  SRR19973062.1768318/150‑1 (MQ=60)
                               TGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCGGCCGTTGCTTGATTCACTGGGGTAACGCTGCATTACCCAACGCCAAGCCGGTGATG                                                                                                                   >  SRR19973062.1694141/1‑150 (MQ=60)
                               TGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCACGACCCCAGCCGGTGCTTGTTGCACAGGGATAAAGCTGCTTTTACCAACGCCAGGCCGGGGATG                                                                                                                   >  SRR19973062.1286744/1‑150 (MQ=60)
                                  GTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCG                                                                                                                <  SRR19973062.4519658/150‑1 (MQ=60)
                                      CGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCGGCCGGTGCTTGATTCACTGGGATAACGCTGGTTTAACCAACGCCATGCCGGGGAGGCCGCCCG                                                                                                            >  SRR19973062.3742911/1‑150 (MQ=60)
                                       GACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCACCAGA                                                                                                           <  SRR19973062.714829/150‑1 (MQ=60)
                                         CACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACT                                                                                                         <  SRR19973062.2636859/150‑1 (MQ=60)
                                         CACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACT                                                                                                         <  SRR19973062.4924648/150‑1 (MQ=60)
                                         CACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACT                                                                                                         <  SRR19973062.1629680/150‑1 (MQ=60)
                                         CACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACT                                                                                                         <  SRR19973062.1629716/150‑1 (MQ=60)
                                            ACGAGAGTTAGTGTTAACCAACGGCGACAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCG                                                                                                      <  SRR19973062.967567/150‑1 (MQ=60)
                                            ACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCGGCCGTTGCATGATGCAATGGGATAACGCAGCGTTACCCAACGCCAAGCCGGGGATGCCCCCAAACTGCG                                                                                                      >  SRR19973062.3555031/1‑150 (MQ=60)
                                             CGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGTTTCACGGGGATAACGCTGCTTTTCCCAACGCCAAGCCGGTGATGCCGCCAGACTGCGA                                                                                                     >  SRR19973062.4849666/1‑150 (MQ=60)
                                                  GTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGGAGCTCGACCGCGGCCGGTGGTTGTTGCACTGGGGTAACGCTGCTTTTCCCAACGCCAagcccgggatggcgccccacggcggggagt                                                                                                >  SRR19973062.4168709/1‑120 (MQ=60)
                                                    TAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTC                                                                                              <  SRR19973062.1557503/150‑1 (MQ=60)
                                                       TGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGC                                                                                           <  SRR19973062.124750/150‑1 (MQ=60)
                                                        GTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCA                                                                                          <  SRR19973062.3728613/150‑1 (MQ=60)
                                                        GTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCA                                                                                          <  SRR19973062.3728604/150‑1 (MQ=60)
                                                        GTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCGCGACCGCTGCCGTTGCGTGTTGCACGGGGGTAACGCTGCATTACCCAACGCCATGCCGGTGAGTCCGCCAGACAGCGAGAAGTTATGCA                                                                                          >  SRR19973062.5346288/1‑150 (MQ=60)
                                                          TAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCGCGACCCCGGCCGGTGCTTGATGCACTGGGGTAAAGCGGCGTTAACCAACGCCAAGCCGGTGATGCCGCCCCACTGCGGGAAGTTCTGGAGC                                                                                        >  SRR19973062.1729908/1‑150 (MQ=60)
                                                           AACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCCCTGCCGGTGCGTGATTCACTGGGATAACGCGGCGGTACCCGACGCCATGCCGGGGATGCCGCCCGACAGCGAGAAGTTATGCAGCC                                                                                       >  SRR19973062.3041278/1‑150 (MQ=60)
                                                              CAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCGGCCGTTGCTTGGTGCACGGGGATAACGCGGCTTTACCCAACGCCATTCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGA                                                                                    >  SRR19973062.3523670/1‑150 (MQ=60)
                                                               AACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCCCTGCCCTTTCGTGAAGCACAGGGCTAACCCAGCTTTACCCAAAGCCCAGCCCGTGATGCCGCCAGACAGCGAGAAGTTATTCAGCCCGAA                                                                                   >  SRR19973062.3099645/1‑150 (MQ=53)
                                                                 CGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACG                                                                                 <  SRR19973062.1734076/150‑1 (MQ=60)
                                                                 CGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACG                                                                                 <  SRR19973062.1734080/150‑1 (MQ=60)
                                                                  GGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGG                                                                                <  SRR19973062.4447161/150‑1 (MQ=60)
                                                                     GAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTTATTCACTGGGATAAAGCTGCTTTAaccaacgccaagacggtgattacgccagaaagcgagtagttcataataccgaaacttaa                                                                             >  SRR19973062.3988910/1‑91 (MQ=60)
                                                                     GAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCA                                                                             <  SRR19973062.911772/150‑1 (MQ=60)
                                                                     GAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCGGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTTCCCAACGCCCGGCCGGTGATGCCGCCCGACAGCGAGAAGTTCTGCAGCCCCAACGGTCA                                                                             >  SRR19973062.898865/1‑150 (MQ=60)
                                                                     GAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCGGCCGGTGGGTGATGGACGGGGGTAAAGCGGCGTTAACCAACGCCCTTCCGGTGTTGCCGCCAGACGTCGAGAAGTTCTGCAGCCCGAACGagat                                                                             >  SRR19973062.2238258/1‑146 (MQ=48)
                                                                       AAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGT                                                                           <  SRR19973062.2541279/150‑1 (MQ=60)
                                                                       AAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCAGCCGTTGGGTGTTGCACTGGGATAACGCTGCTTTACCCAAAGCCAAGCCGGTGTTGCCGCCACACAGCGAGAAGTTCTGCAGCCCGAACCGTCAGT                                                                           >  SRR19973062.431460/1‑150 (MQ=60)
                                                                       AAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCAGCCGTTGCTTGATGCACTGGGATAAAGCTGCTTTAAACAACGCCAAGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACCGTCAGT                                                                           >  SRR19973062.4405210/1‑150 (MQ=60)
                                                                         GCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCCGCGCGACCGCGGCCGGTGCGTTATTCACTGGGTTAAAGCGGCGTTTCCCAACGCCAAGCCGGTGATTCCGCCAGACAGCGAGAAGTTATGCAGCCCGAACGGTCAGGCG                                                                         >  SRR19973062.1726258/1‑150 (MQ=48)
                                                                         GCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCG                                                                         <  SRR19973062.3039267/150‑1 (MQ=60)
                                                                         GCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCG                                                                         <  SRR19973062.863998/150‑1 (MQ=60)
                                                                         GCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCCCTGCCGTTTCTTGATTCACTGGGTTAAAGCTGCTTTTCCCTAAGCCAATCCGGTGGTGCCGCCACACAGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCG                                                                         >  SRR19973062.1726268/1‑150 (MQ=60)
                                                                                CTGGGATCATCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTAT                                                                  <  SRR19973062.679349/150‑1 (MQ=60)
                                                                                CTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTAT                                                                  <  SRR19973062.2302411/150‑1 (MQ=60)
                                                                                CTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCCACCGCGGCCGTTGCTTGATGCACATGGATAACGCTGCATTAACCAAAGCCAAGCCGGTGATGCCGCCACACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTAT                                                                  >  SRR19973062.1352006/1‑150 (MQ=60)
                                                                                 TGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATC                                                                 <  SRR19973062.4064869/150‑1 (MQ=60)
                                                                                  GGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCG                                                                <  SRR19973062.4493631/150‑1 (MQ=60)
                                                                                     ATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCCCTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTG                                                             <  SRR19973062.1065387/150‑1 (MQ=60)
                                                                                     ATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTG                                                             <  SRR19973062.1715778/150‑1 (MQ=60)
                                                                                     ATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTG                                                             <  SRR19973062.3016048/150‑1 (MQ=60)
                                                                                             TTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACT                                                     <  SRR19973062.1517812/150‑1 (MQ=60)
                                                                                             TTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACT                                                     <  SRR19973062.5217030/150‑1 (MQ=60)
                                                                                               TATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTAT                                                   <  SRR19973062.1625773/150‑1 (MQ=60)
                                                                                               TATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTAT                                                   <  SRR19973062.2502212/150‑1 (MQ=60)
                                                                                                  AGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGG                                                <  SRR19973062.2627602/150‑1 (MQ=60)
                                                                                                   GCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGT                                               <  SRR19973062.5236152/150‑1 (MQ=60)
                                                                                                   GCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGT                                               <  SRR19973062.2936147/150‑1 (MQ=60)
                                                                                                      ACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTG                                            <  SRR19973062.4240156/150‑1 (MQ=60)
                                                                                                      ACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTG                                            <  SRR19973062.1740662/150‑1 (MQ=60)
                                                                                                       CCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGG                                           <  SRR19973062.4412257/150‑1 (MQ=60)
                                                                                                           CGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACT                                       <  SRR19973062.67983/150‑1 (MQ=60)
                                                                                                             CGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGG                                     <  SRR19973062.2682465/150‑1 (MQ=60)
                                                                                                             CGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGG                                     <  SRR19973062.2682481/150‑1 (MQ=60)
                                                                                                               GCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCT                                   >  SRR19973062.5298525/1‑150 (MQ=60)
                                                                                                               GCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCT                                   >  SRR19973062.5298543/1‑150 (MQ=60)
                                                                                                               GCAGCTCGACCGCTGCCGTTGCGTGATGCAATGGGATAACGCTGCTTTACCCTACGCCATTCCGGTTAATCCCCCACACTGCCAGAAGTTCTGCAGACCGAACGGTCAGTCGTGATTATCGGTGACGGAACGATTGGTCTGGAACTGTCT                                   >  SRR19973062.2292359/1‑150 (MQ=60)
                                                                                                                  GCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCC                                <  SRR19973062.3666493/150‑1 (MQ=60)
                                                                                                                   CTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCA                               >  SRR19973062.1699047/1‑150 (MQ=60)
                                                                                                                    TCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAG                              >  SRR19973062.2795526/1‑150 (MQ=60)
                                                                                                                          GCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCAC                        <  SRR19973062.3511558/150‑1 (MQ=60)
                                                                                                                          GCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCAC                        >  SRR19973062.4636527/1‑150 (MQ=60)
                                                                                                                           CTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACG                       <  SRR19973062.3808456/150‑1 (MQ=60)
                                                                                                                            TGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGC                      >  SRR19973062.1065773/1‑150 (MQ=60)
                                                                                                                            TGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGC                      >  SRR19973062.3353096/1‑150 (MQ=60)
                                                                                                                            TGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGC                      >  SRR19973062.3777842/1‑150 (MQ=60)
                                                                                                                             GCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCA                     <  SRR19973062.2254026/150‑1 (MQ=60)
                                                                                                                             GCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGAATATCGGTGCCGGAACTATAGGTCTGGAACTGGCTGCAAGCGCCACGCA                     >  SRR19973062.4813058/1‑150 (MQ=60)
                                                                                                                              CCGTTGCTTGATGCACTGGGATAACGCTGCTTGACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAG                    <  SRR19973062.5064476/150‑1 (MQ=60)
                                                                                                                                GTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCG                  >  SRR19973062.3887202/1‑150 (MQ=60)
                                                                                                                                GTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCG                  >  SRR19973062.4679533/1‑150 (MQ=60)
                                                                                                                                 TTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGC                 >  SRR19973062.4819197/1‑150 (MQ=60)
                                                                                                                                  TGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCA                >  SRR19973062.3972152/1‑150 (MQ=60)
                                                                                                                                    CTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGACCTGGCTGCCAGCGCCACGCAGCGCAGA              >  SRR19973062.3198725/1‑150 (MQ=60)
                                                                                                                                     TTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGAT             <  SRR19973062.298365/150‑1 (MQ=60)
                                                                                                                                      TGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATG            >  SRR19973062.4573654/1‑150 (MQ=60)
                                                                                                                                        ATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTA          >  SRR19973062.2520587/1‑150 (MQ=60)
                                                                                                                                        ATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTA          >  SRR19973062.1286395/1‑150 (MQ=60)
                                                                                                                                          GCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAG        >  SRR19973062.4682660/1‑150 (MQ=60)
                                                                                                                                            ACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGT      >  SRR19973062.2589066/1‑150 (MQ=60)
                                                                                                                                             CTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTG     <  SRR19973062.5158532/150‑1 (MQ=60)
                                                                                                                                             CTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTG     <  SRR19973062.3472207/150‑1 (MQ=60)
                                                                                                                                               GGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGAC   <  SRR19973062.1335094/150‑1 (MQ=60)
                                                                                                                                               GGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGAC   <  SRR19973062.4730964/150‑1 (MQ=60)
                                                                                                                                                GGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACA  >  SRR19973062.4085987/1‑150 (MQ=60)
                                                                                                                                                GGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACA  >  SRR19973062.4085989/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                  
CATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACA  >  CP009273/2665625‑2665918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑