Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 625,174 G→T 100% intergenic (+5/‑176) entH → / → cstA enterobactin synthesis proofreading thioesterase/carbon starvation protein involved in peptide utilization; APC peptide transporter family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273625,1740GT97.0% 619.3 / 7.6 214intergenic (+5/‑176)entH/cstAenterobactin synthesis proofreading thioesterase/carbon starvation protein involved in peptide utilization; APC peptide transporter family protein
Reads supporting (aligned to +/- strand):  ref base G (4/3);  new base T (78/123);  total (88/126)
Fisher's exact test for biased strand distribution p-value = 4.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 214 total aligned reads displayed.

CCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAAC  >  CP009273/625029‑625318
                                                                                                                                                 |                                                                                                                                                
  ggTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAg                                                                                                                                              <  1:1500407/148‑1 (MQ=255)
   gtgtCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGa                                                                                                                                             <  2:2882750/148‑1 (MQ=255)
    tgtCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGaa                                                                                                                                            <  1:832390/148‑1 (MQ=255)
     gtCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAAt                                                                                                                                           >  2:3132130/1‑148 (MQ=255)
        tGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAg                                                                                                                                        <  1:1155429/148‑1 (MQ=255)
        tGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAg                                                                                                                                        <  1:3172710/148‑1 (MQ=255)
         gAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGa                                                                                                                                       <  1:2336012/148‑1 (MQ=255)
         gAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGa                                                                                                                                       <  1:2203875/148‑1 (MQ=255)
             gAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATgag                                                                                                                                   <  1:3155086/148‑1 (MQ=255)
               aaGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGAt                                                                                                                                 <  2:2558362/148‑1 (MQ=255)
                  gTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGc                                                                                                                              <  2:2866728/148‑1 (MQ=255)
                             cTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCg                                                                                                                   <  1:1502853/148‑1 (MQ=255)
                              tGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCgg                                                                                                                  >  2:2618473/1‑148 (MQ=255)
                               gCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGt                                                                                                                 <  1:449057/148‑1 (MQ=255)
                               gCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGt                                                                                                                 <  1:553348/148‑1 (MQ=255)
                                ccAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGtt                                                                                                                >  2:3265963/1‑148 (MQ=255)
                                 cAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTa                                                                                                               <  2:2231747/148‑1 (MQ=255)
                                   gCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAAc                                                                                                             >  2:3196594/1‑148 (MQ=255)
                                    ccGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACg                                                                                                            <  1:3267677/148‑1 (MQ=255)
                                     cGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACgg                                                                                                           >  1:2931196/1‑148 (MQ=255)
                                      gCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGa                                                                                                          <  1:2506862/148‑1 (MQ=255)
                                       cTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTGGATTAAAAGGGGCGGCGTTGCTGCACTTGTGGGCTGGGTACGGCAGTTTTGGGTTGTACCCAACAGAATTAGATGAGATTTGAGGAAAAATCGGTTAACCGAg                                                                                                         >  1:617664/1‑148 (MQ=255)
                                       cTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAg                                                                                                         <  2:2301211/148‑1 (MQ=255)
                                        tGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGt                                                                                                        <  2:194998/148‑1 (MQ=255)
                                         gCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTg                                                                                                       <  1:2399547/148‑1 (MQ=255)
                                         gCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTg                                                                                                       <  1:1457145/148‑1 (MQ=255)
                                          cATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGa                                                                                                      <  1:1027045/148‑1 (MQ=255)
                                           aTCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACACGGTTAACGGAGTGAt                                                                                                     >  2:1321289/1‑148 (MQ=255)
                                                 gTCGGCAAACTCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGtt                                                                                               <  2:882008/148‑1 (MQ=255)
                                                     gCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACa                                                                                           <  1:1984567/148‑1 (MQ=255)
                                                      cAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACAt                                                                                          <  1:167182/148‑1 (MQ=255)
                                                      cAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACAt                                                                                          >  2:3419511/1‑148 (MQ=255)
                                                        aaaTCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTg                                                                                        >  2:1467707/1‑148 (MQ=255)
                                                        aaaTCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTg                                                                                        >  2:1469888/1‑148 (MQ=255)
                                                          aTCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTTGGATGAACCCTACAGTATTAGATGAGATTGCAGGAAAACTAGGTTAAAGGAGTGCTAGAGTTAACATTgtt                                                                                      >  1:380483/1‑148 (MQ=255)
                                                            cAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGCTGAAACCAACAGAAATAGATGAGATTGCAGGAAAACACGGTTAAAGGAGTGATCGAGTTAACATTgttaa                                                                                    >  1:258020/1‑148 (MQ=255)
                                                             agagCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTgttaag                                                                                   >  2:1033660/1‑148 (MQ=255)
                                                             agagCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTgttaag                                                                                   <  2:2660022/148‑1 (MQ=255)
                                                              gagCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTgttaagt                                                                                  <  2:1137733/148‑1 (MQ=255)
                                                              gagCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACGTTTCGGCTGGGTACGGCAGTTTTTGGATGAACCCAACAGAATTAGCTGAGATTGCAGGAAAACACGGTTAAACGAGTGATCGAGTTAACATTgtttatt                                                                                  >  1:1601531/1‑144 (MQ=255)
                                                                 cggggAAATCGTCGTGGTCGACGACCAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTgttaagttaa                                                                               <  2:2368301/146‑1 (MQ=255)
                                                                 cTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTgttaagttaa                                                                               >  2:1710126/1‑148 (MQ=255)
                                                                      aaaTCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATAtt                                                                          >  2:358695/1‑148 (MQ=255)
                                                                      aaaTCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATAtt                                                                          >  2:2595506/1‑148 (MQ=255)
                                                                       aaTCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTTGGATGAACCCTACACAAATATATGAGATTGCAGGAAAACTCGGTTAACCGAGTGATCGAGTTAAAATTGTTAAGTTAAATAttt                                                                         >  1:765915/1‑147 (MQ=255)
                                                                        aTCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGATTGATCGAGTTAACATTGTTAAGTTAAATATTgg                                                                        >  1:889358/1‑148 (MQ=255)
                                                                        aTCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTgg                                                                        <  1:2851864/148‑1 (MQ=255)
                                                                         tcgtcgTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGTGATGAACCCTACATAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGt                                                                       <  2:3014207/148‑1 (MQ=255)
                                                                         tcgtcgTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGt                                                                       <  2:2121656/148‑1 (MQ=255)
                                                                             cgtTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCa                                                                   <  1:729269/148‑1 (MQ=255)
                                                                             cgtTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCa                                                                   >  1:1821197/1‑148 (MQ=255)
                                                                                tttCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACt                                                                >  2:1168642/1‑148 (MQ=255)
                                                                                    gATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGa                                                            <  2:488445/148‑1 (MQ=255)
                                                                                    gATGAACAGGGGCGGCGTTGCTGCACTTGTCGACTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGa                                                            <  2:2021763/148‑1 (MQ=255)
                                                                                     aTGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGAt                                                           <  1:3500334/148‑1 (MQ=255)
                                                                                     aTGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGAt                                                           <  1:955081/148‑1 (MQ=255)
                                                                                      tGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGAtt                                                          >  1:3523421/1‑148 (MQ=255)
                                                                                       gAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGAttt                                                         >  1:717506/1‑148 (MQ=255)
                                                                                       gAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGAttt                                                         <  2:1678531/148‑1 (MQ=255)
                                                                                       gAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGAttt                                                         >  1:2159089/1‑148 (MQ=255)
                                                                                        aaCAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTACCATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTa                                                        >  2:3100869/1‑148 (MQ=255)
                                                                                        aaCAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTa                                                        >  2:1284064/1‑148 (MQ=255)
                                                                                          cAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACa                                                      <  2:1927312/148‑1 (MQ=255)
                                                                                           aGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACAt                                                     <  1:620009/148‑1 (MQ=255)
                                                                                            ggggCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATg                                                    >  2:2023191/1‑148 (MQ=255)
                                                                                             gggCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTATATGAGATTGCAGGAAAACTCGGTTAAAGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATgg                                                   >  1:694473/1‑148 (MQ=255)
                                                                                             gggCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATgg                                                   >  2:1760498/1‑148 (MQ=255)
                                                                                                cggcgTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGAGTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTg                                                <  1:389562/148‑1 (MQ=255)
                                                                                                   cgTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCtg                                             <  2:805930/148‑1 (MQ=255)
                                                                                                   cgTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCtg                                             >  2:694543/1‑148 (MQ=255)
                                                                                                     ttGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCtgtg                                           >  2:1205710/1‑148 (MQ=255)
                                                                                                     ttGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCtgtg                                           <  1:2282557/148‑1 (MQ=255)
                                                                                                     ttGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCtgtg                                           <  1:423369/148‑1 (MQ=255)
                                                                                                      tgctgcACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTgtgt                                          >  1:2037377/1‑148 (MQ=255)
                                                                                                         tgcACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGtgttgt                                       <  1:1741362/148‑1 (MQ=255)
                                                                                                         tgcACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGtgttgt                                       <  1:2677355/148‑1 (MQ=255)
                                                                                                          gcACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTgttgtt                                      >  1:2304151/1‑148 (MQ=255)
                                                                                                             cTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTaaa                                   >  2:3493933/1‑148 (MQ=255)
                                                                                                             cTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTaaa                                   <  2:135367/148‑1 (MQ=255)
                                                                                                             cTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTaaa                                   <  1:1760498/148‑1 (MQ=255)
                                                                                                              ttGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAAt                                  <  2:1269229/148‑1 (MQ=255)
                                                                                                                    gCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACa                            >  2:3085220/1‑148 (MQ=255)
                                                                                                                       gggTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAg                         >  1:1920075/1‑148 (MQ=255)
                                                                                                                       gggTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAg                         <  2:1241973/148‑1 (MQ=255)
                                                                                                                        ggTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGa                        <  2:263456/148‑1 (MQ=255)
                                                                                                                        ggTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGa                        <  2:2059541/148‑1 (MQ=255)
                                                                                                                        ggTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGa                        <  1:3132130/148‑1 (MQ=255)
                                                                                                                          tACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATg                      >  1:1222815/1‑148 (MQ=255)
                                                                                                                              gCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTtat                  <  1:2883266/148‑1 (MQ=255)
                                                                                                                              gCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTtat                  <  1:3280379/148‑1 (MQ=255)
                                                                                                                                      gggATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACaa          <  1:469888/148‑1 (MQ=255)
                                                                                                                                       ggATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACaaa         <  1:3286242/148‑1 (MQ=255)
                                                                                                                                       ggATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACaaa         >  1:3274837/1‑148 (MQ=255)
                                                                                                                                       ggATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACaaa         >  1:1072255/1‑148 (MQ=255)
                                                                                                                                         aTGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTTGTTTCAACTCCGATTTACATGTTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAAt       >  2:1082182/1‑148 (MQ=255)
                                                                                                                                         aTGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAAt       >  1:869592/1‑148 (MQ=255)
                                                                                                                                         aTGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAAt       >  1:2650881/1‑148 (MQ=255)
                                                                                                                                          tGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATg      <  2:1079473/148‑1 (MQ=255)
                                                                                                                                           gAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGt     >  1:1247862/1‑148 (MQ=255)
                                                                                                                                              cccTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAAc  >  2:2997362/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAAC  >  CP009273/625029‑625318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAACATCT  >  CP009273/625031‑625322
                                                                                                                                               |                                                                                                                                                    
GGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAA                                                                                                                                                <  SRR19973067.1510290/150‑1 (MQ=60)
GGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAA                                                                                                                                                <  SRR19973067.1510283/150‑1 (MQ=60)
  TGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATT                                                                                                                                              <  SRR19973067.838056/150‑1 (MQ=60)
      TGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGAT                                                                                                                                          <  SRR19973067.1054387/150‑1 (MQ=60)
      TGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGAT                                                                                                                                          <  SRR19973067.46435/150‑1 (MQ=60)
      TGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGAT                                                                                                                                          <  SRR19973067.1163141/150‑1 (MQ=60)
      TGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGAT                                                                                                                                          <  SRR19973067.3192795/150‑1 (MQ=60)
      TGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGAT                                                                                                                                          <  SRR19973067.3190871/150‑1 (MQ=60)
       GAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATG                                                                                                                                         <  SRR19973067.2351316/150‑1 (MQ=60)
       GAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATG                                                                                                                                         <  SRR19973067.2218575/150‑1 (MQ=60)
           GAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGAT                                                                                                                                     <  SRR19973067.3175080/150‑1 (MQ=60)
           GAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGAT                                                                                                                                     <  SRR19973067.3174414/150‑1 (MQ=60)
           GAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGAT                                                                                                                                     <  SRR19973067.1726600/150‑1 (MQ=60)
             AAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTG                                                                                                                                   <  SRR19973067.1800498/150‑1 (MQ=60)
                        CGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACGTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCCACAGAATTAGTGGAGATTGCAGGAAAACTC                                                                                                                        >  SRR19973067.264801/1‑150 (MQ=60)
                           CTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGT                                                                                                                     <  SRR19973067.1512742/150‑1 (MQ=60)
                           CTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGT                                                                                                                     <  SRR19973067.1512746/150‑1 (MQ=60)
                             GCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTA                                                                                                                   <  SRR19973067.556987/150‑1 (MQ=60)
                             GCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTA                                                                                                                   <  SRR19973067.556959/150‑1 (MQ=60)
                             GCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTA                                                                                                                   <  SRR19973067.452176/150‑1 (MQ=60)
                                AGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACG                                                                                                                <  SRR19973067.776714/150‑1 (MQ=60)
                                 GCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGG                                                                                                               >  SRR19973067.2949812/1‑150 (MQ=60)
                                  CCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGA                                                                                                              <  SRR19973067.3288067/150‑1 (MQ=60)
                                  CCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGA                                                                                                              <  SRR19973067.3288066/150‑1 (MQ=60)
                                   CGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTGGATTAAAAGGGGCGGCGTTGCTGCACTTGTGGGCTGGGTACGGCAGTTTTGGGTTGTACCCAACAGAATTAGATGAGATTTGAGGAAAAATCGGTTAACCGAG                                                                                                             >  SRR19973067.621713/1‑150 (MQ=60)
                                   CGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTGTTCGATGAACACGGGCGGCGTTGCTGCACTTGTCGGCTGGGGACGGCAGTTTTGGGATGAACCCTACAGAATTATATGAGATTTGAGGAAAACTAGGTTAAacgag                                                                                                             >  SRR19973067.621457/1‑145 (MQ=60)
                                    GCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGT                                                                                                            <  SRR19973067.2523307/150‑1 (MQ=60)
                                    GCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGT                                                                                                            <  SRR19973067.1005746/150‑1 (MQ=60)
                                      TGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTTGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGA                                                                                                          >  SRR19973067.3094883/1‑150 (MQ=60)
                                       GCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGAT                                                                                                         <  SRR19973067.2415189/150‑1 (MQ=60)
                                       GCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGAT                                                                                                         <  SRR19973067.1466899/150‑1 (MQ=60)
                                        CATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATC                                                                                                        <  SRR19973067.1034107/150‑1 (MQ=60)
                                         ATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACATGTCGGCTGGGTACGGCAGTTTTTGGATGAACCCTACAGAATTATATGAGATTTCAGGAAAAAACGGTTAAAGGAGTGATAG                                                                                                       >  SRR19973067.413956/1‑150 (MQ=60)
                                           CTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAG                                                                                                     <  SRR19973067.2936570/150‑1 (MQ=60)
                                           CTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAG                                                                                                     <  SRR19973067.1152449/150‑1 (MQ=60)
                                             TGGTCGGCTAAATCAGAGCTTGGAAATCGTCGTTTTCGATGAACAGTGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGTATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTT                                                                                                   <  SRR19973067.2038898/150‑1 (MQ=60)
                                             TGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTT                                                                                                   <  SRR19973067.2038880/150‑1 (MQ=60)
                                                   GCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATT                                                                                             <  SRR19973067.1997993/150‑1 (MQ=60)
                                                   GCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATT                                                                                             <  SRR19973067.1998000/150‑1 (MQ=60)
                                                    CAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTG                                                                                            <  SRR19973067.168542/150‑1 (MQ=60)
                                                    CAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTG                                                                                            <  SRR19973067.167503/150‑1 (MQ=60)
                                                    CAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTG                                                                                            >  SRR19973067.2353059/1‑150 (MQ=60)
                                                     AAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGT                                                                                           <  SRR19973067.2323664/150‑1 (MQ=60)
                                                      AAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTTGGATGAACCCTACAGTATTAGATGAGATTGCAGGAAAACTAGGTTAAAGGAGTGCTAGAGTTAACATTGTT                                                                                          >  SRR19973067.383135/1‑150 (MQ=60)
                                                        ATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGCTGAAACCAACAGAAATAGATGAGATTGCAGGAAAACACGGTTAAAGGAGTGATCGAGTTAACATTGTTAA                                                                                        >  SRR19973067.259783/1‑150 (MQ=60)
                                                          CAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGGCCGTGTGGGCTGGGTACGGCAGTTTTTGGGTGTACCCGACAGAATTAGATGAGATTTCAGGAAAAAACGGTTAAAGGAGTGATCGAGTTAACATTGTTAAAT                                                                                      >  SRR19973067.1612523/1‑150 (MQ=60)
                                                          CAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACGTTTCGGCTGGGTACGGCAGTTTTTGGATGAACCCAACAGAATTAGCTGAGATTGCAGGAAAACACGGTTAAACGAGTGATCGAGTTAACATTGTTtatt                                                                                      >  SRR19973067.1612526/1‑146 (MQ=60)
                                                           AGAGCTGGGAAATCGTCGTTTTCGATTAACAGGGGCGGCGTTTCTTCACATGTGGGCTGGGTACGGCAGTTTTGGGATGAAACCTACAGAAATAGATTAGATTTAAGGAAAAAAAGGTTAACGGAGTGTTCGAGTTAAAAATGTTAAGGT                                                                                     >  SRR19973067.3369544/1‑150 (MQ=58)
                                                           AGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTT                                                                                     <  SRR19973067.312619/150‑1 (MQ=60)
                                                            GAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTA                                                                                    <  SRR19973067.846259/150‑1 (MQ=60)
                                                                TGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCATCACATTTCGGCTGGGTACGGCAGTTTTGGGATTAACCCAACAGAAATAGATGAGATTGCAGGAAAACACGGTTAAacgaatgctacagttaaaaattttaagttaaaaa                                                                                >  SRR19973067.1182629/1‑116 (MQ=60)
                                                                  GGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATT                                                                              <  SRR19973067.3506846/150‑1 (MQ=60)
                                                                   GAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTTGGATGAACCCTACACAAATATATGAGATTGCAGGAAAACTCGGTTAACCGAGTGATCGAGTTAAAATTGTTAAGTTAAATATTT                                                                             >  SRR19973067.771235/1‑150 (MQ=60)
                                                                   GAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTT                                                                             >  SRR19973067.772005/1‑150 (MQ=60)
                                                                    AAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTTGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGG                                                                            >  SRR19973067.1463613/1‑150 (MQ=60)
                                                                    AAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGATTGATCGAGTTAACATTGTTAAGTTAAATATTGG                                                                            >  SRR19973067.895252/1‑150 (MQ=60)
                                                                    AAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGG                                                                            >  SRR19973067.895247/1‑150 (MQ=60)
                                                                      ATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTT                                                                          <  SRR19973067.2870204/150‑1 (MQ=60)
                                                                      ATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTT                                                                          <  SRR19973067.2870156/150‑1 (MQ=60)
                                                                       TCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTT                                                                         >  SRR19973067.2244166/1‑150 (MQ=60)
                                                                         GTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCA                                                                       >  SRR19973067.1833539/1‑150 (MQ=60)
                                                                         GTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCA                                                                       >  SRR19973067.3284309/1‑150 (MQ=60)
                                                                           CGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAAC                                                                     <  SRR19973067.734342/150‑1 (MQ=60)
                                                                           CGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAAC                                                                     <  SRR19973067.734337/150‑1 (MQ=60)
                                                                                  GATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATT                                                              <  SRR19973067.1594689/150‑1 (MQ=60)
                                                                                  GATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATT                                                              >  SRR19973067.3545705/1‑150 (MQ=60)
                                                                                   ATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTT                                                             <  SRR19973067.961620/150‑1 (MQ=60)
                                                                                   ATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTT                                                             <  SRR19973067.1527359/150‑1 (MQ=60)
                                                                                   ATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTT                                                             >  SRR19973067.722479/1‑150 (MQ=60)
                                                                                   ATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTT                                                             >  SRR19973067.2173525/1‑150 (MQ=60)
                                                                                   ATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTT                                                             <  SRR19973067.3522598/150‑1 (MQ=60)
                                                                                   ATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTT                                                             <  SRR19973067.3522530/150‑1 (MQ=60)
                                                                                   ATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTT                                                             <  SRR19973067.3302107/150‑1 (MQ=60)
                                                                                   ATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGAGGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTT                                                             >  SRR19973067.2173592/1‑150 (MQ=60)
                                                                                       ACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACAT                                                         <  SRR19973067.28104/150‑1 (MQ=60)
                                                                                        CAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATG                                                        <  SRR19973067.686867/150‑1 (MQ=60)
                                                                                        CAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATG                                                        <  SRR19973067.2481758/150‑1 (MQ=60)
                                                                                         AGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTATATGAGATTGCAGGAAAACTCGGTTAAAGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGG                                                       >  SRR19973067.699267/1‑150 (MQ=60)
                                                                                         AGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGG                                                       <  SRR19973067.624087/150‑1 (MQ=60)
                                                                                              CGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCT                                                  <  SRR19973067.392243/150‑1 (MQ=60)
                                                                                              CGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGAGTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCT                                                  <  SRR19973067.392279/150‑1 (MQ=60)
                                                                                                  GTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGT                                              >  SRR19973067.2051015/1‑150 (MQ=60)
                                                                                                   TTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTT                                             <  SRR19973067.2297650/150‑1 (MQ=60)
                                                                                                   TTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTT                                             <  SRR19973067.2297671/150‑1 (MQ=60)
                                                                                                   TTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTT                                             <  SRR19973067.426313/150‑1 (MQ=60)
                                                                                                    TGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTG                                            >  SRR19973067.71993/1‑150 (MQ=60)
                                                                                                     GCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGT                                           >  SRR19973067.3575538/1‑150 (MQ=60)
                                                                                                      CTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTT                                          >  SRR19973067.2319333/1‑150 (MQ=60)
                                                                                                       TGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTA                                         <  SRR19973067.2694511/150‑1 (MQ=60)
                                                                                                       TGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTA                                         <  SRR19973067.1753258/150‑1 (MQ=60)
                                                                                                       TGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTA                                         <  SRR19973067.437705/150‑1 (MQ=60)
                                                                                                       TGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTA                                         <  SRR19973067.2694521/150‑1 (MQ=60)
                                                                                                         CACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAA                                       >  SRR19973067.249327/1‑150 (MQ=60)
                                                                                                          ACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAAT                                      >  SRR19973067.2146483/1‑150 (MQ=60)
                                                                                                           CTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATT                                     <  SRR19973067.1772482/150‑1 (MQ=60)
                                                                                                              GTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTA                                  <  SRR19973067.1876573/150‑1 (MQ=60)
                                                                                                                   CTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAG                             >  SRR19973067.1933175/1‑150 (MQ=60)
                                                                                                                     GGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGAT                           <  SRR19973067.759972/150‑1 (MQ=60)
                                                                                                                      GGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATG                          >  SRR19973067.535604/1‑150 (MQ=60)
                                                                                                                      GGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATG                          <  SRR19973067.3152032/150‑1 (MQ=60)
                                                                                                                      GGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATG                          >  SRR19973067.1230961/1‑150 (MQ=60)
                                                                                                                        TACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTT                        <  SRR19973067.898708/150‑1 (MQ=60)
                                                                                                                            GCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAG                    <  SRR19973067.2901705/150‑1 (MQ=60)
                                                                                                                            GCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAG                    <  SRR19973067.3300941/150‑1 (MQ=60)
                                                                                                                                  TTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAA              >  SRR19973067.1760463/1‑150 (MQ=60)
                                                                                                                                   TGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAA             >  SRR19973067.3295327/1‑150 (MQ=60)
                                                                                                                                   TGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAA             >  SRR19973067.1079609/1‑150 (MQ=60)
                                                                                                                                   TGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAA             >  SRR19973067.3295419/1‑150 (MQ=60)
                                                                                                                                    GGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAA            <  SRR19973067.473140/150‑1 (MQ=60)
                                                                                                                                     GGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAAT           >  SRR19973067.2667973/1‑150 (MQ=60)
                                                                                                                                     GGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAAT           <  SRR19973067.2659112/150‑1 (MQ=60)
                                                                                                                                     GGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAAT           <  SRR19973067.3306874/150‑1 (MQ=60)
                                                                                                                                     GGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAAT           >  SRR19973067.875404/1‑150 (MQ=60)
                                                                                                                                      GATGAACCCTACAGAATTAGATGAGATTGCAGGAGAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATG          >  SRR19973067.2550912/1‑150 (MQ=60)
                                                                                                                                       ATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGT         >  SRR19973067.1256281/1‑150 (MQ=60)
                                                                                                                                         GAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAA       <  SRR19973067.12425/150‑1 (MQ=60)
                                                                                                                                              CTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAACATCT  >  SRR19973067.963868/1‑150 (MQ=60)
                                                                                                                                              CTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAACATCT  >  SRR19973067.2752016/1‑150 (MQ=60)
                                                                                                                                               |                                                                                                                                                    
GGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAACATCT  >  CP009273/625031‑625322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑