Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 625,174 G→T 100% intergenic (+5/‑176) entH → / → cstA enterobactin synthesis proofreading thioesterase/carbon starvation protein involved in peptide utilization; APC peptide transporter family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273625,1740GT96.2% 581.2 / 12.6 211intergenic (+5/‑176)entH/cstAenterobactin synthesis proofreading thioesterase/carbon starvation protein involved in peptide utilization; APC peptide transporter family protein
Reads supporting (aligned to +/- strand):  ref base G (4/5);  new base T (106/90);  total (116/95)
Fisher's exact test for biased strand distribution p-value = 7.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 211 total aligned reads displayed.

CCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAAC  >  CP009273/625029‑625318
                                                                                                                                                 |                                                                                                                                                
   gtgtCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGa                                                                                                                                             <  1:1980971/148‑1 (MQ=255)
     gtCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAAt                                                                                                                                           <  1:2317293/148‑1 (MQ=255)
      tCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAAtt                                                                                                                                          <  1:557402/148‑1 (MQ=255)
       cTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTa                                                                                                                                         <  1:2901198/148‑1 (MQ=255)
       cTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTa                                                                                                                                         >  1:1428653/1‑148 (MQ=255)
           gggAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGTGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATg                                                                                                                                     <  2:372311/148‑1 (MQ=255)
            ggAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATga                                                                                                                                    <  1:690723/148‑1 (MQ=255)
            ggAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATga                                                                                                                                    <  1:2478466/148‑1 (MQ=255)
             gAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATgag                                                                                                                                   <  2:1651012/148‑1 (MQ=255)
              aaaGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATgagc                                                                                                                                  >  2:790409/1‑147 (MQ=255)
               aaGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTTTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGAt                                                                                                                                 >  1:2661528/1‑148 (MQ=255)
               aaGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGAt                                                                                                                                 >  2:2252061/1‑148 (MQ=255)
                aGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGAtt                                                                                                                                <  2:520530/148‑1 (MQ=255)
                  gTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGc                                                                                                                              >  2:3199165/1‑148 (MQ=255)
                       cggcgTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACGTGGCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGACTTAGATGAGATTGCAGGaa                                                                                                                         >  2:1481886/1‑148 (MQ=255)
                           gTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACt                                                                                                                     <  1:2473594/148‑1 (MQ=255)
                            tCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTc                                                                                                                    >  2:2440158/1‑148 (MQ=255)
                             cTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCg                                                                                                                   >  1:1474074/1‑148 (MQ=255)
                              tGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCgg                                                                                                                  <  2:215327/148‑1 (MQ=255)
                                    ccGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACg                                                                                                            >  1:832402/1‑148 (MQ=255)
                                       cTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATTAGATTTCAGGAAAAATCGGTTAAAGGAg                                                                                                         >  1:1596221/1‑148 (MQ=255)
                                       cTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAAATCGGTTAACGGAg                                                                                                         >  1:1367689/1‑148 (MQ=255)
                                       cTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAAACCTACAGAATTAGATGAGATTTCAGGAAAAAGCGGGTAAAGGAg                                                                                                         >  1:3366936/1‑148 (MQ=255)
                                         gCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTg                                                                                                       <  1:2162324/148‑1 (MQ=255)
                                         gCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTg                                                                                                       >  2:340012/1‑148 (MQ=255)
                                         gCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCAACAGAATTAGATGAGATTGCAGGAAAAATCGGTTAACGGAGTg                                                                                                       >  2:3464709/1‑148 (MQ=255)
                                          cATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCCGCGGTGCTGCACTTGTCGGCGGGGTACGGCAGTTTTGGGATGAACCCTACAGACTTAGATGAGATTGCCGGAAAACTCGGTTAACGGAGTGa                                                                                                      >  2:1846192/1‑148 (MQ=255)
                                             cTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGGACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCg                                                                                                   >  1:275432/1‑148 (MQ=255)
                                             cTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCg                                                                                                   >  2:185085/1‑148 (MQ=255)
                                             cTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCg                                                                                                   >  1:2977869/1‑148 (MQ=255)
                                                ggTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGt                                                                                                >  2:3159851/1‑148 (MQ=255)
                                                   cGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTaa                                                                                             >  2:1091977/1‑148 (MQ=255)
                                                      cAAAATCAGAGCTGGGAAATCGTCGTGTTCGATGAAAAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAAACCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACAt                                                                                          >  1:2808892/1‑148 (MQ=255)
                                                        aaaTCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAAACCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTg                                                                                        >  2:717329/1‑148 (MQ=255)
                                                            cAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAAATAGATGAGATTGCAGGAAAAATCGGTTAAACGAGTGATAGAGTTAAAAATgttaa                                                                                    >  1:440015/1‑148 (MQ=255)
                                                               agCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTgttaagtt                                                                                 >  2:2716607/1‑148 (MQ=255)
                                                                gCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTgttaagtta                                                                                <  1:1144748/148‑1 (MQ=255)
                                                                 cTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGATATTGCATGAAAACTAGGTTAACGGAGTGATCGAGTTAAAATTgttaagttaa                                                                               >  2:2550958/1‑148 (MQ=255)
                                                                 cTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTgttaatttaa                                                                               >  2:2393928/1‑148 (MQ=255)
                                                                 cTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTgttaagttaa                                                                               >  2:2061041/1‑148 (MQ=255)
                                                                  tGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTaaa                                                                              <  2:864592/148‑1 (MQ=255)
                                                                   gggAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAat                                                                             >  2:2930277/1‑148 (MQ=255)
                                                                   gggAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAat                                                                             >  2:2761951/1‑148 (MQ=255)
                                                                    ggAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAata                                                                            >  2:1746170/1‑148 (MQ=255)
                                                                       aaTCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTg                                                                         <  2:296312/148‑1 (MQ=255)
                                                                       aaTCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTg                                                                         >  2:1621462/1‑148 (MQ=255)
                                                                        aTCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTgg                                                                        <  1:445758/148‑1 (MQ=255)
                                                                        aTCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTgg                                                                        >  2:2179321/1‑148 (MQ=255)
                                                                         tcgtcgTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGt                                                                       >  2:2915729/1‑148 (MQ=255)
                                                                            tcggTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTc                                                                    >  1:3227696/1‑148 (MQ=255)
                                                                             cgtTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCa                                                                   >  2:3091065/1‑148 (MQ=255)
                                                                               ttttCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAAc                                                                 >  1:3014634/1‑148 (MQ=255)
                                                                                 ttCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGGTTCAACTc                                                               >  2:15382/1‑148 (MQ=255)
                                                                                   cGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCg                                                             <  1:3458580/148‑1 (MQ=255)
                                                                                    gATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGa                                                            <  2:762363/148‑1 (MQ=255)
                                                                                     aTGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGAt                                                           >  2:1684739/1‑148 (MQ=255)
                                                                                       gAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGAttt                                                         <  1:237942/148‑1 (MQ=255)
                                                                                         aCAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTAc                                                       <  2:415737/148‑1 (MQ=255)
                                                                                           aGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACAt                                                     >  1:2790267/1‑148 (MQ=255)
                                                                                             gggCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATgg                                                   >  2:1215797/1‑148 (MQ=255)
                                                                                             gggCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATgg                                                   >  2:61257/1‑148 (MQ=255)
                                                                                             gggCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCAAAAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTTTTAAGTTAAATATTGGTTTCAACTCCGATTTACAttg                                                   >  1:2772669/1‑146 (MQ=255)
                                                                                                   cgTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCtg                                             <  1:1884084/148‑1 (MQ=255)
                                                                                                    gTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCtgt                                            <  2:1997079/148‑1 (MQ=255)
                                                                                                     ttGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCtgtg                                           <  2:3495023/148‑1 (MQ=255)
                                                                                                     ttGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCtgtg                                           >  2:3024547/1‑148 (MQ=255)
                                                                                                      tgctgcACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTgtgt                                          <  1:353651/148‑1 (MQ=255)
                                                                                                      tgctgcACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTgtgt                                          >  1:1776807/1‑148 (MQ=255)
                                                                                                       gctgcACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGtgtt                                         <  2:442985/148‑1 (MQ=255)
                                                                                                         tgcACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGtgttgt                                       >  1:1082953/1‑148 (MQ=255)
                                                                                                           cACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTa                                     <  1:405132/148‑1 (MQ=255)
                                                                                                              ttGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAAt                                  <  2:3257261/148‑1 (MQ=255)
                                                                                                               tGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAAtt                                 <  1:1015708/148‑1 (MQ=255)
                                                                                                               tGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAAtt                                 <  2:3323979/148‑1 (MQ=255)
                                                                                                               tGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAAtt                                 <  2:2530296/148‑1 (MQ=255)
                                                                                                                 tCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGt                               >  2:1290929/1‑148 (MQ=255)
                                                                                                                 tCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGt                               >  2:3236166/1‑148 (MQ=255)
                                                                                                                     cTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACaa                           >  2:3214860/1‑148 (MQ=255)
                                                                                                                       gggTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAg                         <  2:2374995/148‑1 (MQ=255)
                                                                                                                       gggTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAg                         <  1:1219883/148‑1 (MQ=255)
                                                                                                                        ggTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGa                        >  1:2725266/1‑148 (MQ=255)
                                                                                                                         gTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGAt                       <  1:3338278/148‑1 (MQ=255)
                                                                                                                             ggCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTta                   <  2:1473893/148‑1 (MQ=255)
                                                                                                                             ggCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTta                   <  1:609789/148‑1 (MQ=255)
                                                                                                                              gCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTtat                  <  1:3084298/148‑1 (MQ=255)
                                                                                                                              gCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTTTTGTTAAATTGTACAAAGATGTtat                  <  1:815558/148‑1 (MQ=255)
                                                                                                                                  ttttGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGaa              <  1:3199165/148‑1 (MQ=255)
                                                                                                                                  ttttGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGaa              <  1:2227251/148‑1 (MQ=255)
                                                                                                                                  ttttGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGaa              >  2:3516488/1‑148 (MQ=255)
                                                                                                                                   tttGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGaaa             <  1:1704015/148‑1 (MQ=255)
                                                                                                                                    ttGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAAc            >  1:3543518/1‑148 (MQ=255)
                                                                                                                                       ggATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACaaa         >  2:1786129/1‑148 (MQ=255)
                                                                                                                                       ggATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACaaa         <  1:2269008/148‑1 (MQ=255)
                                                                                                                                            aaCCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTa    <  1:2313588/148‑1 (MQ=255)
                                                                                                                                             aCCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTaa   <  1:2896909/148‑1 (MQ=255)
                                                                                                                                              cccTACAGACTTAGATGAGATTGCAGGAAAACTCGGGTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAAc  >  2:2320286/1‑148 (MQ=255)
                                                                                                                                              cccTACAGAATTAGATGATATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAATTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAAc  <  2:2661528/148‑1 (MQ=255)
                                                                                                                                              cccTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAAc  >  1:3125137/1‑148 (MQ=255)
                                                                                                                                              cccTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAAc  <  1:1274893/148‑1 (MQ=255)
                                                                                                                                              cccTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAAc  <  2:1299705/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAAC  >  CP009273/625029‑625318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAACAT  >  CP009273/625025‑625320
                                                                                                                                                     |                                                                                                                                                  
TCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCT                                                                                                                                                    <  SRR19973066.796992/150‑1 (MQ=60)
TCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCT                                                                                                                                                    <  SRR19973066.796544/150‑1 (MQ=60)
      GGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAA                                                                                                                                              >  SRR19973066.3552304/1‑150 (MQ=60)
      GGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGACAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAA                                                                                                                                              >  SRR19973066.2490758/1‑150 (MQ=60)
       GTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAAT                                                                                                                                             <  SRR19973066.1994955/150‑1 (MQ=60)
        TGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATT                                                                                                                                            <  SRR19973066.2863113/150‑1 (MQ=60)
         GTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTA                                                                                                                                           >  SRR19973066.1438858/1‑150 (MQ=60)
         GTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTA                                                                                                                                           <  SRR19973066.2333140/150‑1 (MQ=60)
         GTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTA                                                                                                                                           <  SRR19973066.2333139/150‑1 (MQ=60)
          TCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAG                                                                                                                                          <  SRR19973066.561395/150‑1 (MQ=60)
           CTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGA                                                                                                                                         <  SRR19973066.2920575/150‑1 (MQ=60)
           CTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGA                                                                                                                                         <  SRR19973066.2920582/150‑1 (MQ=60)
                GGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGA                                                                                                                                    <  SRR19973066.695810/150‑1 (MQ=60)
                GGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGA                                                                                                                                    <  SRR19973066.2495379/150‑1 (MQ=60)
                 GAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTTTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGAT                                                                                                                                   >  SRR19973066.2679362/1‑150 (MQ=60)
                 GAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGAT                                                                                                                                   <  SRR19973066.2988437/150‑1 (MQ=60)
                 GAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTGTTCGGTGAACAGGGGCGGGGTTGGTGCACTTGTCGGCTGGGTACGGCACTTTTTGGATGTAACCAAAAGAAATAGATGAGAT                                                                                                                                   >  SRR19973066.418984/1‑150 (MQ=60)
                           CGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAA                                                                                                                         >  SRR19973066.1492093/1‑150 (MQ=60)
                            GGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAAC                                                                                                                        <  SRR19973066.2578114/150‑1 (MQ=60)
                               GTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTTGGATGAAACCAACAGACTTAGATGAGATTTCAGGAAAACTCG                                                                                                                     >  SRR19973066.406633/1‑150 (MQ=60)
                               GTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCG                                                                                                                     >  SRR19973066.1484276/1‑150 (MQ=60)
                               GTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCG                                                                                                                     >  SRR19973066.1484457/1‑150 (MQ=60)
                               GTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCG                                                                                                                     <  SRR19973066.2490461/150‑1 (MQ=60)
                                    CCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAA                                                                                                                <  SRR19973066.1779292/150‑1 (MQ=60)
                                      AGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACG                                                                                                              >  SRR19973066.838471/1‑150 (MQ=60)
                                       GCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGG                                                                                                             <  SRR19973066.3207815/150‑1 (MQ=60)
                                         CGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTTGGATGAACCCTACAGAAATATATGAGATTGCAGGAAAAATCGGTTAAAGGAG                                                                                                           >  SRR19973066.3389364/1‑150 (MQ=60)
                                         CGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATTAGATTTCAGGAAAAATCGGTTAAAGGAG                                                                                                           >  SRR19973066.1607783/1‑150 (MQ=60)
                                         CGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAAATCGGTTAACGGAG                                                                                                           >  SRR19973066.1377582/1‑150 (MQ=60)
                                         CGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAAACCTACAGAATTAGATGAGATTTCAGGAAAAagcgggtaaaggag                                                                                                           >  SRR19973066.3389387/1‑136 (MQ=60)
                                         CGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACGTGTCGGCTGGGTACGGCAGTTTTGGGATGTACCCTACACAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAG                                                                                                           >  SRR19973066.2145064/1‑150 (MQ=60)
                                           CTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTG                                                                                                         <  SRR19973066.714641/150‑1 (MQ=60)
                                            TGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGA                                                                                                        <  SRR19973066.1418395/150‑1 (MQ=60)
                                             GCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGAT                                                                                                       <  SRR19973066.2177350/150‑1 (MQ=60)
                                               ATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGGACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCG                                                                                                     >  SRR19973066.277452/1‑150 (MQ=60)
                                               ATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCG                                                                                                     >  SRR19973066.2997546/1‑150 (MQ=60)
                                               ATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGGTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTTGGATGAAACCAACCGAAATAGATGAGATTTCAGGAAAAaacgggtaaccgcgtgatcg                                                                                                     >  SRR19973066.16580/1‑130 (MQ=60)
                                                    GGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTA                                                                                                <  SRR19973066.3409550/150‑1 (MQ=60)
                                                        GGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACAT                                                                                            <  SRR19973066.868220/150‑1 (MQ=60)
                                                        GGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACAT                                                                                            <  SRR19973066.53984/150‑1 (MQ=60)
                                                        GGCAAAATCAGAGCTGGGAAATCGTCGTGTTCGATGAAAAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAAACCTACAGAAATAGATTAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACAT                                                                                            >  SRR19973066.2827791/1‑150 (MQ=60)
                                                           AAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAAAAGGGGCGGCGGTGCTGCCCTTGTCGGCGGGGTACGGCCGTTTTGGGGTGAAACCGAAAGAATTAGATGAGATTGCAGGAAAACACGGTTAACCGAGTGATCGAGTTAACATTGT                                                                                         >  SRR19973066.2426794/1‑150 (MQ=60)
                                                              ATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAAATAGATGAGATTGCAGGAAAAATCGGTTAAACGAGTGATAGAGTTAAAAATGTTAA                                                                                      >  SRR19973066.443417/1‑150 (MQ=60)
                                                                    GCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAA                                                                                <  SRR19973066.1152924/150‑1 (MQ=60)
                                                                       GGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATAT                                                                             <  SRR19973066.2305708/150‑1 (MQ=60)
                                                                        GGAAATCGTCGTTTTCGATGAAAAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGTTGAACCCTACACAAATAGATTAGATTTCAGGAAAAATCGGTTAACGGAGTGATCGAGTTAAAAATGTTAATTTAAAAATT                                                                            >  SRR19973066.2746939/1‑150 (MQ=60)
                                                                           AATCGTCGTTTTAGATTAAAAGGGGCGGCGTTTCTGCACATGTAGGCTGGGTACGGCACTTTTTGGATGAACCCAACAGAAAAAGATTAGATTTCAGGAAAACTCGGTTAAAGGATTGATCGAGTTAAAAATGTTAAGTTAAATATTGGT                                                                         >  SRR19973066.2738703/1‑150 (MQ=60)
                                                                            ATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTT                                                                        <  SRR19973066.449189/150‑1 (MQ=60)
                                                                             TCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTT                                                                       <  SRR19973066.1059082/150‑1 (MQ=60)
                                                                             TCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTT                                                                       <  SRR19973066.2242313/150‑1 (MQ=60)
                                                                             TCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTT                                                                       <  SRR19973066.1237326/150‑1 (MQ=60)
                                                                              CGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTC                                                                      >  SRR19973066.3249087/1‑150 (MQ=60)
                                                                              CGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACAATGTTAAGTTAAATATTGGTTTC                                                                      >  SRR19973066.3249023/1‑150 (MQ=60)
                                                                              CGTCGGTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTC                                                                      >  SRR19973066.3249032/1‑150 (MQ=60)
                                                                               GTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCA                                                                     >  SRR19973066.570665/1‑150 (MQ=60)
                                                                                 CGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAAC                                                                   >  SRR19973066.3034723/1‑150 (MQ=60)
                                                                                   TTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTC                                                                 >  SRR19973066.446410/1‑150 (MQ=60)
                                                                                       CGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGAT                                                             >  SRR19973066.2379922/1‑150 (MQ=60)
                                                                                       CGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGAT                                                             <  SRR19973066.3481606/150‑1 (MQ=60)
                                                                                       CGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGAT                                                             <  SRR19973066.3481708/150‑1 (MQ=60)
                                                                                          TGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTA                                                          <  SRR19973066.2267591/150‑1 (MQ=60)
                                                                                           GAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTAC                                                         <  SRR19973066.239809/150‑1 (MQ=60)
                                                                                             ACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACAT                                                       >  SRR19973066.2809043/1‑150 (MQ=60)
                                                                                             ACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACAT                                                       >  SRR19973066.2807341/1‑150 (MQ=60)
                                                                                               AGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGG                                                     >  SRR19973066.2791325/1‑150 (MQ=60)
                                                                                               AGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCAAAAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTTTTAAGTTAAATATTGGTTTCAACTCCGATTTACATTG                                                     >  SRR19973066.2791337/1‑150 (MQ=60)
                                                                                                       CGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTG                                             <  SRR19973066.1897524/150‑1 (MQ=60)
                                                                                                        GTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGT                                            >  SRR19973066.1789512/1‑150 (MQ=60)
                                                                                                          TGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTG                                          <  SRR19973066.356404/150‑1 (MQ=60)
                                                                                                           GCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGT                                         >  SRR19973066.1090861/1‑150 (MQ=60)
                                                                                                               CACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAA                                     <  SRR19973066.408320/150‑1 (MQ=60)
                                                                                                                ACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAAT                                    <  SRR19973066.3053302/150‑1 (MQ=60)
                                                                                                                   TGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGT                                 <  SRR19973066.1023186/150‑1 (MQ=60)
                                                                                                                         CTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAG                           >  SRR19973066.2998143/1‑150 (MQ=60)
                                                                                                                         CTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAG                           >  SRR19973066.1210456/1‑150 (MQ=60)
                                                                                                                          TGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGA                          >  SRR19973066.2743597/1‑150 (MQ=60)
                                                                                                                           GGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGAT                         <  SRR19973066.1228498/150‑1 (MQ=60)
                                                                                                                            GGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATG                        <  SRR19973066.2474801/150‑1 (MQ=60)
                                                                                                                            GGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATG                        <  SRR19973066.2740918/150‑1 (MQ=60)
                                                                                                                             GTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGT                       <  SRR19973066.3360498/150‑1 (MQ=60)
                                                                                                                               ACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTA                     <  SRR19973066.112423/150‑1 (MQ=60)
                                                                                                                                 GGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATA                   <  SRR19973066.614035/150‑1 (MQ=60)
                                                                                                                                  GCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAG                  <  SRR19973066.3104905/150‑1 (MQ=60)
                                                                                                                                  GCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTTTTGTTAAATTGTACAAAGATGTTATAG                  <  SRR19973066.821946/150‑1 (MQ=60)
                                                                                                                                  GCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTTTTGTTAAATTGTACAAAGATGTTATAG                  <  SRR19973066.821535/150‑1 (MQ=60)
                                                                                                                                   CAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGA                 <  SRR19973066.1933021/150‑1 (MQ=60)
                                                                                                                                    AGTTTTGGGATGAACCCTACAGAATTATATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAA                >  SRR19973066.1750980/1‑150 (MQ=60)
                                                                                                                                    AGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAA                >  SRR19973066.186285/1‑150 (MQ=60)
                                                                                                                                      TTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAAC              >  SRR19973066.3567037/1‑150 (MQ=60)
                                                                                                                                      TTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAAC              <  SRR19973066.2242640/150‑1 (MQ=60)
                                                                                                                                      TTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAAC              >  SRR19973066.3567049/1‑150 (MQ=60)
                                                                                                                                      TTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAAC              <  SRR19973066.3220408/150‑1 (MQ=60)
                                                                                                                                       TTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACA             <  SRR19973066.1716387/150‑1 (MQ=60)
                                                                                                                                          GGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAA          >  SRR19973066.1326852/1‑150 (MQ=60)
                                                                                                                                          GGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAA          >  SRR19973066.674972/1‑150 (MQ=60)
                                                                                                                                           GGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAAT         <  SRR19973066.2284613/150‑1 (MQ=60)
                                                                                                                                            GATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTTTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATG        >  SRR19973066.2816713/1‑150 (MQ=60)
                                                                                                                                                AACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACCAAATGTAAC    >  SRR19973066.3145846/1‑150 (MQ=60)
                                                                                                                                                AACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAAC    <  SRR19973066.725629/150‑1 (MQ=60)
                                                                                                                                                AACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAAC    <  SRR19973066.2329420/150‑1 (MQ=60)
                                                                                                                                                AACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAAC    >  SRR19973066.3146006/1‑150 (MQ=60)
                                                                                                                                                 ACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAACA   >  SRR19973066.2688672/1‑150 (MQ=60)
                                                                                                                                                 ACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAACA   <  SRR19973066.2916272/150‑1 (MQ=60)
                                                                                                                                                 ACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAACA   <  SRR19973066.2916322/150‑1 (MQ=60)
                                                                                                                                                  CCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAACAT  <  SRR19973066.1284101/150‑1 (MQ=60)
                                                                                                                                                  CCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAACAT  >  SRR19973066.2700769/1‑150 (MQ=60)
                                                                                                                                                  CCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAACAT  >  SRR19973066.2700800/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                  
TCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAACAT  >  CP009273/625025‑625320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑