Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F361 I0 R1 1353 250.7 8230166 96.0% 7900959 148.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 625,174 G→T 100% intergenic (+5/‑176) entH → / → cstA enterobactin synthesis proofreading thioesterase/carbon starvation protein involved in peptide utilization; APC peptide transporter family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273625,1740GT98.0% 690.6 / 3.4 234intergenic (+5/‑176)entH/cstAenterobactin synthesis proofreading thioesterase/carbon starvation protein involved in peptide utilization; APC peptide transporter family protein
Reads supporting (aligned to +/- strand):  ref base G (5/1);  new base T (85/137);  total (96/138)
Fisher's exact test for biased strand distribution p-value = 3.66e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 234 total aligned reads displayed.

CCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAACA  >  CP009273/625029‑625319
                                                                                                                                                 |                                                                                                                                                 
ccGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACa                                                                                                                                                >  2:2196798/1‑149 (MQ=255)
 cGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAg                                                                                                                                               <  1:1214650/149‑1 (MQ=255)
 cGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAg                                                                                                                                               >  2:3206427/1‑149 (MQ=255)
         gtggtaaaGGTACGCGGCGTCTGCCTGCGGCTGAATCTTGGTCGGAAAAATATTTGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGAt                                                                                                                                       <  1:967591/144‑1 (MQ=255)
         gAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGAt                                                                                                                                       <  1:1969314/149‑1 (MQ=255)
         gAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGAt                                                                                                                                       <  1:3045120/149‑1 (MQ=255)
         gAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTCGGGATGAACCCTACAGAATTAGAt                                                                                                                                       <  1:1855332/149‑1 (MQ=255)
           gggAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATga                                                                                                                                     <  2:1727444/149‑1 (MQ=255)
           gggAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATga                                                                                                                                     <  1:4019017/149‑1 (MQ=255)
           gggAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATga                                                                                                                                     >  1:3427870/1‑149 (MQ=255)
            ggAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATgag                                                                                                                                    <  2:904025/149‑1 (MQ=255)
              aaaGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGAt                                                                                                                                  <  2:1278449/149‑1 (MQ=255)
              aaaGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGAt                                                                                                                                  <  1:3357618/149‑1 (MQ=255)
              aaaGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTAGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTTGGATGAAACCTACAGAAATAGATGAGAt                                                                                                                                  >  1:1569407/1‑149 (MQ=255)
               aaGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGAtt                                                                                                                                 <  2:3528020/149‑1 (MQ=255)
               aaGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGAtt                                                                                                                                 <  2:3846494/149‑1 (MQ=255)
                   tACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAg                                                                                                                             <  1:381357/149‑1 (MQ=255)
                      gcggcgTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGaa                                                                                                                          <  1:92104/149‑1 (MQ=255)
                        ggcgTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGaaaa                                                                                                                        <  1:1789990/149‑1 (MQ=255)
                         gcgTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAAc                                                                                                                       >  2:1503493/1‑149 (MQ=255)
                         gcgTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTAGATTAACAGGGGCGGCGTTGCTTCACTTGTGGGCTGGGGTCGGCAGTTTTTGGATTAAACCGACAGAATTATATGAGATTTGAGGaaaaa                                                                                                                       >  1:398633/1‑148 (MQ=255)
                            tCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTGTTAGATGAACAGGGGCGGCGGTGCTGCCCGTTTCGGCGGGGTACGGCCGTTTTTGGGTGAAACCAACAGAATTATATGAGATTGCAGGaaaaaaag                                                                                                                    >  1:1743417/1‑145 (MQ=255)
                               gCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGtt                                                                                                                 >  1:3761362/1‑149 (MQ=255)
                               gCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGtt                                                                                                                 >  1:2564407/1‑149 (MQ=255)
                                ccAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTa                                                                                                                <  1:1665021/149‑1 (MQ=255)
                                 cAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTaa                                                                                                               >  2:2899222/1‑149 (MQ=255)
                                   gCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACg                                                                                                             >  1:1290636/1‑149 (MQ=255)
                                   gCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACg                                                                                                             <  1:3412036/149‑1 (MQ=255)
                                   gCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTAAGGCAGTTTTGGGATTTACCCAACCGAATTAGATGAGATTGGAGGAAAACTCGGTTAACg                                                                                                             >  1:1502495/1‑149 (MQ=255)
                                     cGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGa                                                                                                           <  2:1307876/149‑1 (MQ=255)
                                      gCTGCATCTTGGTCGACAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAg                                                                                                          <  1:60484/149‑1 (MQ=255)
                                        tGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTg                                                                                                        >  2:50199/1‑149 (MQ=255)
                                        tGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTAGATGAACAGGGGCGGCGTTGCTGCACTTGTAGGCTGGGTACGGCAGTTTTTGGATGTACCCTACAGAATTAGATGAGATTGCAGGAAAAATCGGTTAAAGGAGTg                                                                                                        >  1:3943748/1‑149 (MQ=255)
                                           aTCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATc                                                                                                     >  2:2291995/1‑149 (MQ=255)
                                            tCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCg                                                                                                    <  2:3179414/149‑1 (MQ=255)
                                             cTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGa                                                                                                   <  2:2335091/149‑1 (MQ=255)
                                              ttGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAg                                                                                                  <  2:3027476/149‑1 (MQ=255)
                                              ttGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAg                                                                                                  >  1:2366926/1‑149 (MQ=255)
                                              ttGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAg                                                                                                  <  1:3311133/149‑1 (MQ=255)
                                                    ggCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACa                                                                                            >  2:2044567/1‑149 (MQ=255)
                                                     gCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACAt                                                                                           >  2:1394932/1‑149 (MQ=255)
                                                       aaaaTCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGGTGGGTACGGCAGTTTTTGGATGTACCCTACAGAATTAGATGAGATTGCAGGAAAAAACGGTTAACGGAGTGATAGAGTTAACAATg                                                                                         >  1:3826209/1‑149 (MQ=255)
                                                       aaaaTCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACACGGTTAAAGGAGTGAACGAGTTAAAAttt                                                                                         >  2:2069021/1‑148 (MQ=255)
                                                         aaTCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTgtt                                                                                       <  1:3284018/149‑1 (MQ=255)
                                                         aaTCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTgtt                                                                                       <  1:3282124/149‑1 (MQ=255)
                                                         aaTCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCGGGGTACGGCAGTTTTGGGATGAAACCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTgtt                                                                                       >  2:3684389/1‑149 (MQ=255)
                                                          aTCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGTTGGGTACGGCAGTTTTTGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAAAATTgtta                                                                                      >  1:2314421/1‑149 (MQ=255)
                                                          aTCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACGTGTCGGTTGGGTACGGCAGTTTTTGGATGAACCCAACACAATTATATGAGATTTCAGGTAAACTCGGTTAAAGGAGTGATAGAGTTAAAATTgtta                                                                                      >  1:430570/1‑149 (MQ=255)
                                                             agagCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTgttaagt                                                                                   <  2:1138959/149‑1 (MQ=255)
                                                             agagCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTgttaagt                                                                                   >  2:2393661/1‑149 (MQ=255)
                                                              gagCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTgttaagtt                                                                                  <  1:1487548/149‑1 (MQ=255)
                                                              gagCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTgttaagtt                                                                                  <  2:2985617/149‑1 (MQ=255)
                                                                  tGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAat                                                                              <  2:146730/149‑1 (MQ=255)
                                                                    ggAAATCGTCGTTTTCGATGAACTGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAatat                                                                            <  2:3390241/149‑1 (MQ=255)
                                                                          cgtcgtTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGttt                                                                      <  2:1224301/149‑1 (MQ=255)
                                                                            tcgtTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCa                                                                    <  2:1998724/149‑1 (MQ=255)
                                                                                tttCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTc                                                                <  1:1377916/149‑1 (MQ=255)
                                                                                  tCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCg                                                              <  1:2059952/149‑1 (MQ=255)
                                                                                  tCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCg                                                              >  1:281136/1‑149 (MQ=255)
                                                                                  tCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCg                                                              <  1:1391404/149‑1 (MQ=255)
                                                                                   cGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGa                                                             <  2:2662884/149‑1 (MQ=255)
                                                                                    gATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGAt                                                            <  1:1073171/149‑1 (MQ=255)
                                                                                      tGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGAttt                                                          <  1:520585/149‑1 (MQ=255)
                                                                                       gAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGACTTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTa                                                         >  2:2648083/1‑149 (MQ=255)
                                                                                       gAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTa                                                         <  1:2464421/149‑1 (MQ=255)
                                                                                       gAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTa                                                         <  1:723083/149‑1 (MQ=255)
                                                                                         aCAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACa                                                       <  1:2636772/149‑1 (MQ=255)
                                                                                         aCAGGGGCGGCGGTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTAGAGAATTAGTTGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACa                                                       <  2:3473742/149‑1 (MQ=255)
                                                                                           aGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTATATATTGGTTTCAACTCCGATTTACATg                                                     >  2:3990584/1‑149 (MQ=255)
                                                                                           aGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATg                                                     >  2:602034/1‑149 (MQ=255)
                                                                                             gggCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGTAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGGTTCAACTCCGATTTACATGGt                                                   <  1:2717747/149‑1 (MQ=255)
                                                                                                  gcgTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCtg                                              >  1:1632666/1‑149 (MQ=255)
                                                                                                     ttGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTgtgt                                           <  2:1049709/149‑1 (MQ=255)
                                                                                                     ttGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTgtgt                                           <  1:1405101/149‑1 (MQ=255)
                                                                                                        ctgcACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGtgttgt                                        <  1:3589452/149‑1 (MQ=255)
                                                                                                             cTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAAt                                   <  2:2194157/149‑1 (MQ=255)
                                                                                                             cTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAAt                                   <  1:3746649/149‑1 (MQ=255)
                                                                                                             cTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAAt                                   <  2:3968688/149‑1 (MQ=255)
                                                                                                              ttGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAAtt                                  <  1:2707007/149‑1 (MQ=255)
                                                                                                                       gggTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGa                         >  2:2512318/1‑149 (MQ=255)
                                                                                                                       gggTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGa                         >  1:287142/1‑149 (MQ=255)
                                                                                                                          tACTGCAGTTTTGGTATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATAGAGTTAACATTTTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGt                      <  2:2230602/149‑1 (MQ=255)
                                                                                                                          tACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGt                      >  1:460493/1‑149 (MQ=255)
                                                                                                                           aCGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGtt                     <  2:3428409/149‑1 (MQ=255)
                                                                                                                            cGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTta                    >  1:1107139/1‑149 (MQ=255)
                                                                                                                             ggCAGTTTTGGGATGAACCCTCCAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTCAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTtat                   <  2:1644931/149‑1 (MQ=255)
                                                                                                                             ggCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTtat                   <  2:2791957/149‑1 (MQ=255)
                                                                                                                             ggCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTtat                   >  2:1217066/1‑149 (MQ=255)
                                                                                                                              gCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTtata                  >  2:1892913/1‑149 (MQ=255)
                                                                                                                              gCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTtata                  >  2:1385516/1‑149 (MQ=255)
                                                                                                                                  ttttGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGaaa              <  2:4099367/149‑1 (MQ=255)
                                                                                                                                  ttttGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGaaa              >  2:661183/1‑149 (MQ=255)
                                                                                                                                    tttggATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACa            >  1:3855256/4‑149 (MQ=255)
                                                                                                                                     tGGGATGAACCCTACAGAATTAGATGAGATTGCAGTAAAACTAGTTTAACGGAGTGATCTAGTTAACATTGTTAAGTTAAATATTGTTTTCAACTCCGATTTACATGGTTGCTTTGTTGTTAATTTGTACAAAGATGTTATAGAAACaa           <  2:2471341/149‑1 (MQ=255)
                                                                                                                                        gATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAAt        <  1:114063/149‑1 (MQ=255)
                                                                                                                                           gAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGTTGTTATAGAAACAAAATGTa     >  1:2167225/1‑149 (MQ=255)
                                                                                                                                            aaCCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTaa    <  2:3761362/149‑1 (MQ=255)
                                                                                                                                            aaCCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTaa    >  2:626558/1‑149 (MQ=255)
                                                                                                                                             aCCCTACAGACTTAGATGAGAATGCAGGAAAACTCGGTTAACGGACTGATCGAGTAAACATTGTTAAGTTAAATATTGGTTTCAACTCAGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAAc   >  2:2763327/1‑149 (MQ=255)
                                                                                                                                             aCCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAAc   >  1:1278365/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
CCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAACA  >  CP009273/625029‑625319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAACATCTC  >  CP009273/625026‑625323
                                                                                                                                                    |                                                                                                                                                     
CGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTA                                                                                                                                                      <  SRR19973068.1219243/150‑1 (MQ=60)
CGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTA                                                                                                                                                      <  SRR19973068.1219237/150‑1 (MQ=60)
    CGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGA                                                                                                                                                  <  SRR19973068.1222737/150‑1 (MQ=60)
    CGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGGTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTTGGATGAACCCAACAGA                                                                                                                                                  >  SRR19973068.2104916/1‑150 (MQ=60)
       TGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATT                                                                                                                                               <  SRR19973068.3772296/150‑1 (MQ=60)
          CTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGA                                                                                                                                            >  SRR19973068.124940/1‑150 (MQ=60)
            gtggtAAAGGTACGCGGCGTCTGCCTGCGGCTGAATCTTGGTCGGAAAAATATTTGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATG                                                                                                                                          <  SRR19973068.974021/145‑1 (MQ=60)
            GAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATG                                                                                                                                          <  SRR19973068.1982367/150‑1 (MQ=60)
            GAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATG                                                                                                                                          <  SRR19973068.3063788/150‑1 (MQ=60)
            GAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATG                                                                                                                                          <  SRR19973068.973987/150‑1 (MQ=60)
            GAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTCGGGATGAACCCTACAGAATTAGATG                                                                                                                                          <  SRR19973068.1867589/150‑1 (MQ=60)
             AGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGA                                                                                                                                         >  SRR19973068.3448527/1‑150 (MQ=60)
              GGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAG                                                                                                                                        <  SRR19973068.4043272/150‑1 (MQ=60)
                GAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTAGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTTGGATGAAACCTACAGAAATAGATGAGAT                                                                                                                                      >  SRR19973068.1579731/1‑150 (MQ=60)
                 AAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATT                                                                                                                                     <  SRR19973068.3378047/150‑1 (MQ=60)
                   AGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGC                                                                                                                                   <  SRR19973068.708882/150‑1 (MQ=60)
                   AGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGC                                                                                                                                   <  SRR19973068.3492486/150‑1 (MQ=60)
                     GTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGCTGAACCCTACAGAATTAGATGAGATTTCAG                                                                                                                                 >  SRR19973068.1598317/1‑150 (MQ=60)
                      TACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGG                                                                                                                                <  SRR19973068.1412536/150‑1 (MQ=60)
                      TACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGG                                                                                                                                <  SRR19973068.384969/150‑1 (MQ=60)
                      TACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGG                                                                                                                                <  SRR19973068.383974/150‑1 (MQ=60)
                      TACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGG                                                                                                                                <  SRR19973068.1481173/150‑1 (MQ=60)
                         GCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAA                                                                                                                             <  SRR19973068.93050/150‑1 (MQ=60)
                         GCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAA                                                                                                                             <  SRR19973068.3385510/150‑1 (MQ=60)
                           GGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAAC                                                                                                                           <  SRR19973068.1801670/150‑1 (MQ=60)
                           GGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTAGATTAACAGGGGCGGCGTTGCTTCACTTGTGGGCTGGGGTCGGCAGTTTTTGGATTAAACCGACAGAATTATATGAGATTtgaggaaaaa                                                                                                                           >  SRR19973068.401419/1‑140 (MQ=60)
                              GTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTGTTAGATGAACAGGGGCGGCGGTGCTGCCCGTTTCGGCGGGGTACGGCCGTTTTTGGGTGAAACCAACAGAATTATATGAGATTGCAGGAAAAaaag                                                                                                                        >  SRR19973068.1754517/1‑146 (MQ=60)
                                 TGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGCATTAGATGATATTGCAGGAAAACTCGGTT                                                                                                                     >  SRR19973068.248913/1‑150 (MQ=60)
                                 TGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTTCAGGAAAACTCGGTT                                                                                                                     >  SRR19973068.2580943/1‑150 (MQ=60)
                                 TGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTT                                                                                                                     <  SRR19973068.584953/150‑1 (MQ=60)
                                 TGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTT                                                                                                                     >  SRR19973068.3783834/1‑150 (MQ=60)
                                 TGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTT                                                                                                                     >  SRR19973068.2580655/1‑150 (MQ=60)
                                  GCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTAGATGAACAGGGGCGGCGTTGGTGCACTTGTGGGCTGGGTCAGGCAGTTTTTGGCTGAAACCTACAGAAATAGATGATATTGGAGGAAAAaaaggtta                                                                                                                    >  SRR19973068.2455649/1‑142 (MQ=60)
                                   CCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAA                                                                                                                   <  SRR19973068.1675781/150‑1 (MQ=60)
                                    CAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAAC                                                                                                                  <  SRR19973068.909832/150‑1 (MQ=60)
                                     AGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGGTGCCCTTGTCGGCTGGGTACGGCAGTTTTTGGGTGAACCCAACACAATTAGATGAGATTTCCGGAAAACACGGGTAACG                                                                                                                 >  SRR19973068.3127389/1‑150 (MQ=60)
                                     AGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTTTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAAATCGGTTAAAG                                                                                                                 >  SRR19973068.3879141/1‑150 (MQ=60)
                                     AGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGATATTGCAGGAAAAATCGGTTAAAG                                                                                                                 >  SRR19973068.1299123/1‑150 (MQ=60)
                                     AGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTTCAGGAAAACTCGGTTAACG                                                                                                                 >  SRR19973068.1299113/1‑150 (MQ=60)
                                     AGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTAAGGCAGTTTTGGGATTTACCCAACCGAATTAGATGAGATTGGAGGAAAACTCGGTTAACG                                                                                                                 >  SRR19973068.1512373/1‑150 (MQ=60)
                                      GCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGG                                                                                                                <  SRR19973068.3432645/150‑1 (MQ=60)
                                      GCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGG                                                                                                                <  SRR19973068.3434084/150‑1 (MQ=60)
                                      GCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGG                                                                                                                <  SRR19973068.1845504/150‑1 (MQ=60)
                                         GCTGCATCTTGGTCGACAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGT                                                                                                             <  SRR19973068.61195/150‑1 (MQ=60)
                                          CTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTAGATGAACAGGGGCGGCGTTGCTGCACTTGTAGGCTGGGTACGGCAGTTTTTGGATGTACCCTACAGAATTAGATGAGATTGCAGGAAAAATCGGTTAAAGGAGTG                                                                                                            >  SRR19973068.3967625/1‑150 (MQ=60)
                                           TGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGA                                                                                                           <  SRR19973068.453759/150‑1 (MQ=60)
                                             CATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATC                                                                                                         <  SRR19973068.1112224/150‑1 (MQ=60)
                                             CATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATC                                                                                                         <  SRR19973068.2369936/150‑1 (MQ=60)
                                             CATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATC                                                                                                         <  SRR19973068.2369024/150‑1 (MQ=60)
                                              ATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGGTTCTGCACATGTCGGCTGGGTACGGCAGTTTTTGGATTAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGTTCG                                                                                                        >  SRR19973068.1188335/1‑150 (MQ=60)
                                                CTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAG                                                                                                      >  SRR19973068.2382064/1‑150 (MQ=60)
                                                 TTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGT                                                                                                     <  SRR19973068.3331371/150‑1 (MQ=60)
                                                       GGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAAATAGATGAGATTTCAGGAAAAATCGGTTAACGGAGTGGTCGAGTTAAaaa                                                                                               >  SRR19973068.841434/1‑147 (MQ=60)
                                                       GGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCGGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACAT                                                                                               >  SRR19973068.2914520/1‑150 (MQ=60)
                                                         CAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGGTGGGTACGGCAGTTTTTGGATGTACCCTACAGAATTAGATGAGATTGCAGGAAAAAACGGTTAACGGAGTGATAGAGTTAACAATG                                                                                             >  SRR19973068.3849259/1‑150 (MQ=60)
                                                            AATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGTTGGGTACGGCAGTTTTTGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAAAATTGTTA                                                                                          >  SRR19973068.2329352/1‑150 (MQ=60)
                                                            AATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTA                                                                                          <  SRR19973068.3302268/150‑1 (MQ=60)
                                                            AATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTA                                                                                          <  SRR19973068.3302250/150‑1 (MQ=60)
                                                            AATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTA                                                                                          <  SRR19973068.3304155/150‑1 (MQ=60)
                                                            AATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACGTGTCGGTTGGGTACGGCAGTTTTTGGATGAACCCAACACAATTATATGAGATTTCAGGTAAACTCGGTTAAAGGAGTGATAGAGTTAAAATTGTTA                                                                                          >  SRR19973068.433622/1‑150 (MQ=60)
                                                             ATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTNA                                                                                         <  SRR19973068.1755625/150‑1 (MQ=60)
                                                             ATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAA                                                                                         <  SRR19973068.1755615/150‑1 (MQ=60)
                                                              TCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAG                                                                                        <  SRR19973068.3162157/150‑1 (MQ=60)
                                                                AGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGGTGGGTACGGGCGTTTTTGGATGAACCCGACACAATTAGATGAGATTGCAGGAAAAATCCGGTAAAGGAGGGATCGAGTTAAaattttttaggt                                                                                      >  SRR19973068.2110449/1‑138 (MQ=60)
                                                                AGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACGTGTCGGCGGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTT                                                                                      >  SRR19973068.1709638/1‑150 (MQ=60)
                                                                 GAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTA                                                                                     <  SRR19973068.1497321/150‑1 (MQ=60)
                                                                 GAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTA                                                                                     <  SRR19973068.2790348/150‑1 (MQ=60)
                                                                        GAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGATATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAATTTAAATATTG                                                                              >  SRR19973068.1396792/1‑150 (MQ=60)
                                                                         AAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTTCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGG                                                                             >  SRR19973068.2714517/1‑150 (MQ=60)
                                                                         AAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGG                                                                             <  SRR19973068.3970953/150‑1 (MQ=60)
                                                                         AAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGG                                                                             <  SRR19973068.138866/150‑1 (MQ=60)
                                                                           ATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTT                                                                           <  SRR19973068.3807776/150‑1 (MQ=60)
                                                                                 GTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACT                                                                     <  SRR19973068.1504500/150‑1 (MQ=60)
                                                                                 GTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACT                                                                     <  SRR19973068.2887260/150‑1 (MQ=60)
                                                                                 GTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACT                                                                     >  SRR19973068.3339624/1‑150 (MQ=60)
                                                                                   TTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCC                                                                   <  SRR19973068.1386693/150‑1 (MQ=60)
                                                                                    TTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCG                                                                  >  SRR19973068.283074/1‑150 (MQ=60)
                                                                                     TCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGA                                                                 <  SRR19973068.4119446/150‑1 (MQ=60)
                                                                                     TCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGA                                                                 <  SRR19973068.2073344/150‑1 (MQ=60)
                                                                                     TCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGA                                                                 <  SRR19973068.1400265/150‑1 (MQ=60)
                                                                                       GATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATT                                                               <  SRR19973068.1080239/150‑1 (MQ=60)
                                                                                         TGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTA                                                             <  SRR19973068.524184/150‑1 (MQ=60)
                                                                                          GAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTAC                                                            >  SRR19973068.3318754/1‑150 (MQ=60)
                                                                                          GAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTAC                                                            <  SRR19973068.2480148/150‑1 (MQ=60)
                                                                                          GAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTAC                                                            <  SRR19973068.2480144/150‑1 (MQ=60)
                                                                                          GAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTAC                                                            <  SRR19973068.727821/150‑1 (MQ=60)
                                                                                            ACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACAT                                                          <  SRR19973068.2653333/150‑1 (MQ=60)
                                                                                                GGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGTAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGGTTCAACTCCGATTTACATGGTT                                                      <  SRR19973068.2734560/150‑1 (MQ=60)
                                                                                                GGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTT                                                      >  SRR19973068.1997455/1‑150 (MQ=60)
                                                                                                GGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTT                                                      <  SRR19973068.2734569/150‑1 (MQ=60)
                                                                                                    GGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTG                                                  >  SRR19973068.1643326/1‑150 (MQ=60)
                                                                                                    GGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTG                                                  >  SRR19973068.1643309/1‑150 (MQ=60)
                                                                                                    GGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTG                                                  <  SRR19973068.1186642/150‑1 (MQ=60)
                                                                                                     GCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGT                                                 <  SRR19973068.1585541/150‑1 (MQ=60)
                                                                                                     GCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGT                                                 <  SRR19973068.4028160/150‑1 (MQ=60)
                                                                                                      CGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTG                                                >  SRR19973068.1471913/1‑150 (MQ=60)
                                                                                                        TTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTT                                              <  SRR19973068.1414149/150‑1 (MQ=60)
                                                                                                        TTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTT                                              <  SRR19973068.1868287/150‑1 (MQ=60)
                                                                                                           CTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTT                                           <  SRR19973068.3611254/150‑1 (MQ=60)
                                                                                                             GCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAA                                         <  SRR19973068.1126240/150‑1 (MQ=60)
                                                                                                                CTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATT                                      <  SRR19973068.3769075/150‑1 (MQ=60)
                                                                                                                CTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATT                                      <  SRR19973068.3768847/150‑1 (MQ=60)
                                                                                                                 TTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTG                                     <  SRR19973068.2723815/150‑1 (MQ=60)
                                                                                                                 TTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTG                                     <  SRR19973068.3852553/150‑1 (MQ=60)
                                                                                                                 TTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTG                                     <  SRR19973068.2723793/150‑1 (MQ=60)
                                                                                                                      GGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAA                                >  SRR19973068.3833253/1‑150 (MQ=60)
                                                                                                                      GGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAA                                >  SRR19973068.2594835/1‑150 (MQ=60)
                                                                                                                         TGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGA                             >  SRR19973068.289101/1‑150 (MQ=60)
                                                                                                                           GGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATG                           <  SRR19973068.408176/150‑1 (MQ=60)
                                                                                                                            GTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGT                          >  SRR19973068.463740/1‑150 (MQ=60)
                                                                                                                            GTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGT                          <  SRR19973068.1576346/150‑1 (MQ=60)
                                                                                                                              ACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTA                        >  SRR19973068.1114518/1‑150 (MQ=60)
                                                                                                                              ACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTA                        <  SRR19973068.3363357/150‑1 (MQ=60)
                                                                                                                                      TTTTGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACA                >  SRR19973068.3878621/1‑150 (MQ=60)
                                                                                                                                      TTTTGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACA                >  SRR19973068.3878535/1‑150 (MQ=60)
                                                                                                                                         GGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAA             >  SRR19973068.2031155/1‑150 (MQ=60)
                                                                                                                                          GGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAAT            >  SRR19973068.1763380/1‑150 (MQ=60)
                                                                                                                                          GGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAAT            <  SRR19973068.2182031/150‑1 (MQ=60)
                                                                                                                                           GATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATG           <  SRR19973068.115159/150‑1 (MQ=60)
                                                                                                                                            ATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGT          >  SRR19973068.585643/1‑150 (MQ=60)
                                                                                                                                             TGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGTTGTTATAGAAACAAAATGTA         >  SRR19973068.2181416/1‑150 (MQ=60)
                                                                                                                                               AACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAAC       >  SRR19973068.1288226/1‑150 (MQ=60)
                                                                                                                                               AACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAAC       >  SRR19973068.1286783/1‑150 (MQ=60)
                                                                                                                                                 CCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAACAT     <  SRR19973068.50808/150‑1 (MQ=60)
                                                                                                                                                   CTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAACATCT   >  SRR19973068.2739050/1‑150 (MQ=60)
                                                                                                                                                   CTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAACATCT   <  SRR19973068.953722/150‑1 (MQ=60)
                                                                                                                                                    TACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAACATATC  >  SRR19973068.3406133/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
CGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAACATCTC  >  CP009273/625026‑625323

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑